A batch disinfection device for a primary fibroblast culture medium

Abstract

The utility model discloses a batch disinfection device of primary fibroblast culture medium, including pipe frame and being located pipe frame intercommunication's water storage shell, be provided with along the vertical movement path movement, and the side wall of water storage shell set up the spout of shower nozzle, still fixed mounting of water storage shell is perpendicular to the side wall of multiple shell plates of setting, and the end of shell plate fixed mounting is located the elastic strip of shower nozzle outside, the batch disinfection device of primary fibroblast culture medium provided by the utility model, through multiple elastic strip to culture bottle outer wall resistance to touch clamping, the bottom inner wall of culture bottle is fixed to the upper end of shower nozzle, realize the placement fixed of culture bottle, then through the pressurized disinfection water in water storage shell and flow into the shower nozzle from the top port, again from multiple spouts to the inner wall of culture bottle, realize the disinfection water spray of culture bottle inner wall, and the water mist produced by spraying can also compensate the rest position of culture bottle inner wall, guarantee the cleaning disinfection effect of culture bottle inner wall.

Description

Technical Field

[0001] This utility model relates to the field of cell culture medium sterilization technology, specifically to a batch sterilization device for primary fibroblast cell culture medium. Background Technology

[0002] Some primary fibroblast culture media are cultured in bottles, and these bottles need to be sterilized during repeated use to ensure that the culture medium is free from contamination.

[0003] According to the publication (announcement) number: CN220426260U, the publication (announcement) date: 2024-02-02, a biological cell culture bottle sterilization and cleaning machine is disclosed, which includes a rotating column equipped with a brush, and the culture bottle is inverted on the outside of the rotating column to use the brush to clean the inner wall of the bottle.

[0004] In the prior art, including the aforementioned patent, dirt remains on the bristles during the brush cleaning process, and the dirt remaining on the bristles also returns to the inner wall of the bottle during the brush washing process, resulting in reduced cleaning efficiency. The bristles also need to be cleaned afterward to prevent cross-contamination of the next batch of culture bottles. Furthermore, rinsing with disinfectant is required after cleaning, which greatly consumes time for disinfection and cleaning operations. Utility Model Content

[0005] The purpose of this invention is to provide a batch sterilization device for primary fibroblast culture medium, which aims to solve the problems mentioned above.

[0006] To achieve the above objectives, this utility model provides the following technical solution:

[0007] A batch sterilization device for primary fibroblast culture medium includes a cleaning tank with a door panel, a tube rack, and a water storage shell connected to the tube rack. The water storage shell is provided with a nozzle that moves along a vertical moving path and has nozzles on its side wall.

[0008] The water storage shell is also fixedly installed with multiple shell plates that are perpendicular to its side wall, and the ends of the shell plates are fixedly installed with elastic strips located outside the nozzle.

[0009] Preferably, the water storage shell is connected to a vertical pipe that slides inside the nozzle.

[0010] Preferably, the vertical pipe has multiple holes that communicate with the nozzle.

[0011] Preferably, a slider that slides within the pipe hole is fixedly installed at the lower port of the nozzle.

[0012] Preferably, a spring located inside the vertical pipe is fixedly installed at the upper end of the nozzle and the water storage tank.

[0013] Preferably, it also includes a circular plate that slides within the water storage tank and is used to open and close the upper port of the water storage tank.

[0014] Preferably, the circular plate has multiple openings, and a guide post that is inserted into and mates with the openings is fixedly installed in the water storage shell.

[0015] Preferably, a nozzle-matching pin is fixedly installed on the circular plate.

[0016] Preferably, the elastic strip is a flexible metal sheet.

[0017] Preferably, the circular plate is a stainless steel metal plate.

[0018] In the above technical solution, the batch sterilization device for primary fibroblast culture medium provided by this utility model has the following beneficial effects: the outer wall of the culture bottle is clamped by multiple elastic strips, and the upper end of the nozzle is clamped against the inner wall of the bottom of the culture bottle to achieve the placement and fixation of the culture bottle. Then, pressurized sterilization water in the water tank flows into the nozzle from the upper port and is sprayed onto the inner wall of the culture bottle from multiple nozzles to achieve the spraying and rinsing of the inner wall of the culture bottle with sterilization water. At the same time, the water mist generated by the spray can also compensate for other positions on the inner wall of the culture bottle, ensuring the cleaning and sterilization effect of the inner wall of the culture bottle, and will not cause pollution to the inner wall of the culture bottle. Attached Figure Description

[0019] To more clearly illustrate the technical solutions in the embodiments of this application or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments recorded in this utility model. For those skilled in the art, other drawings can be obtained based on these drawings.

[0020] Figure 1 A schematic diagram of the cleaning tank with its lid removed and its internal pipe rack assembled with a water storage shell, provided in an embodiment of this utility model;

[0021] Figure 2 This is a schematic diagram of the assembly of a portion of the pipe rack and water storage tank provided in an embodiment of the present utility model;

[0022] Figure 3 This is a partial cross-sectional view of the assembly of the pipe rack and water storage tank provided in an embodiment of the present utility model;

[0023] Figure 4 This is a schematic diagram of the water storage tank and nozzle exploded from above, provided for an embodiment of this utility model.

[0024] Explanation of reference numerals in the attached figures:

[0025] 1. Cleaning tank; 11. Door panel; 12. Pipe rack; 2. Water tank; 21. Shell plate; 22. Elastic strip; 23. Vertical pipe; 24. Pipe hole; 25. Guide post; 3. Nozzle; 31. Nozzle outlet; 32. Slider; 4. Ejector pin; 41. Circular plate; 42. Opening. Detailed Implementation

[0026] To enable those skilled in the art to better understand the technical solution of this utility model, the present utility model will be further described in detail below with reference to the accompanying drawings.

[0027] like Figure 1-4 As shown, a batch sterilization device for primary fibroblast culture medium includes a cleaning box 1 with a door panel 11, a tube rack 12 and a water storage shell 2 connected to the tube rack 12. The water storage shell 2 is provided with a nozzle 3 that moves along a vertical moving path and has a nozzle 31 on its side wall.

[0028] Multiple shell plates 21 are also fixedly installed on the water storage shell 2, which are perpendicular to its side wall. An elastic strip 22 located outside the nozzle 3 is fixedly installed at the end of the shell plate 21.

[0029] Specifically, the door panel 11 is hinged to the cleaning tank 1, which is existing technology. The cleaning tank 1 is equipped with a pressurized pipeline connected to the pipe rack 12 (both the connection method and the pressurization method are existing technologies and will not be described in detail here), so that the disinfectant water enters the channel in the pipe rack 12 and is then temporarily stored in multiple water storage tanks 2.

[0030] Furthermore, the tube rack 12 is arranged equidistantly in the cleaning box 1, and the vertical arrangement allows the culture bottle to be placed upside down on the water storage shell 2. The nozzle 31 atomizes the disinfectant water, and the atomization method is also existing technology, which will not be described in detail here.

[0031] Furthermore, the shell plate 21 is integrally formed and arranged circumferentially at the lower port of the water storage shell 2. At the same time, the shell plate 21 is detachably connected to the pipe rack 12 by bolts and nuts.

[0032] By pressing the port of the culture flask against the shell plate 21, and then using multiple elastic strips 22 with bends at the upper end to press and clamp the outer wall of the culture flask, along with the upper end of the nozzle 3 pressing against the inner wall of the bottom of the culture flask, the culture flask is placed and fixed. Then, pressurized disinfectant water in the water storage shell 2 flows into the nozzle 3 from the upper port, and is sprayed onto the inner wall of the culture flask from multiple nozzles 31, realizing the disinfectant spraying and rinsing of the inner wall of the culture flask. At the same time, the water mist generated by the spray can also compensate for the other parts of the inner wall of the culture flask, ensuring the cleaning and disinfection effect of the inner wall of the culture flask, and will not cause pollution to the inner wall of the culture flask. This achieves efficient and large-scale culture flask disinfection. Finally, the disinfected water falls downwards to the bottom of the cleaning tank 1 for recycling.

[0033] As a further embodiment of this utility model, a vertical pipe 23 that slides inside the nozzle 3 is connected to the water storage shell 2.

[0034] The disinfectant water discharged from the upper port of the water storage tank 2 is transported through the vertical pipe 23, and the nozzle 3 can be adjusted on the vertical pipe 23 to match the length of the culture bottle, so that the cleaning box 1 can be loaded with culture bottles of different specifications for disinfection.

[0035] As another embodiment further provided in this utility model, the vertical pipe 23 is provided with a plurality of pipe holes 24 communicating with the nozzle 31.

[0036] The circularly arranged pipe holes 24 can communicate with the vertically arranged nozzles 31, so that when the nozzle 3 and the water storage shell 2 maintain the maximum distance, the combination of the pipe holes 24 and the nozzles 31 has the maximum water outlet area. Therefore, it can achieve efficient spraying of disinfectant water when adapted to culture bottles of different specifications.

[0037] As another embodiment further provided by this utility model, a slider 32 that slides in the pipe hole 24 is fixedly installed at the lower port of the nozzle 3.

[0038] The slider 32 slides vertically in the pipe hole 24 to allow the nozzle 3 to move stably up and down. After moving down, the pipe hole 24 can also be connected to the nozzle 31, so it will not interfere with the spraying of disinfectant.

[0039] As another embodiment of this utility model, a spring located inside the vertical pipe 23 is fixedly installed on the upper end of the nozzle 3 and the water storage shell 2.

[0040] Specifically, the two ends of the spring are welded to the inner wall of the nozzle 3 and the upper end of the water storage shell 2, respectively.

[0041] The spring stores energy in the downward-moving nozzle 3 to support the weight of the culture bottle and also ensures the nozzle 3 can return to its original position.

[0042] As another embodiment of this utility model, it also includes a circular plate 41 that slides in the water storage tank 2 and is used to open and close the upper port of the water storage tank 2.

[0043] By positioning the circular plate 41 at the top of the water storage tank 2 in its default state and sealing the top opening, the residual disinfectant water in the water storage tank 2 is less likely to be contaminated by external air introduced by the opening and closing of the cleaning tank 1. This also reduces the possibility of disinfectant water remaining on the outside of the water storage tank 2, thus ensuring the safety of the disinfectant water.

[0044] As another embodiment of this utility model, a plurality of openings 42 are provided on the circular plate 41, and a guide post 25 that is inserted into and cooperates with the openings 42 is fixedly installed in the water storage shell 2.

[0045] Specifically, a spring is welded to the lower end face of the circular plate 41. The lower end of the spring abuts against the upper surface of the tube rack 12, and the spring, in its default state, causes the opening 42 to be blocked by the guide post 25.

[0046] Water is supplied to the upper and lower ports of the water storage tank 2 through the opening 42, and the opening and closing are performed in conjunction with the guide post 25.

[0047] As another embodiment provided in this utility model, a pin 4 that cooperates with the nozzle 3 is fixedly installed on the circular plate 41.

[0048] Specifically, in the default state, the spring causes the upper end of the ejector pin 4 to extend beyond the upper end of the nozzle 3.

[0049] During the placement of the culture bottle, pressure is applied to the upper end of the ejector pin 4, and the upper end of the ejector pin 4 and the upper end of the nozzle 3 are aligned and then abut against the inner wall of the culture bottle. At this time, the spring below the circular plate 41 is compressed, and the opening 42 is opened by the guide post 25 to realize the water supply. After the water supply to the tube rack 12 is cut off (the internal disinfectant water can flow with pressure), the culture bottle is used to push the ejector pin 4 and the nozzle 3 up and down repeatedly. Since the nozzle 31 and the tube hole 24 are connected to the inside of the bottle, the circular plate 41 slides on the guide post 25, and a piston movement is formed inside the water storage shell 2, which is used to spray air from the upper port of the water storage shell 2 to act on the inner wall of the culture bottle, so as to realize the function of blowing out a certain amount of air to evaporate water vapor.

[0050] As another embodiment further provided in this utility model, the elastic strip 22 is specifically an elastic metal sheet.

[0051] The elastic metal sheet prevents the elastic strip 22 from causing excessive pressure damage to the outer wall of the culture bottle, while also ensuring the stability of the clamping of the culture bottle.

[0052] As another embodiment further provided in this utility model, the circular plate 41 is specifically a stainless steel metal plate.

[0053] Using stainless steel metal plates reduces corrosion of the circular plate 41 after prolonged contact with disinfectant, thus extending its service life.

[0054] Working principle: The culture bottle is fixed by pressing its port against the shell plate 21, and then using multiple elastic strips 22 with bends at the upper end to press and clamp the outer wall of the culture bottle. At the same time, the upper end of the nozzle 3 presses against the inner wall of the bottom of the culture bottle, thus fixing the culture bottle in place. Then, pressurized disinfectant water in the water storage shell 2 flows into the nozzle 3 from the upper port and is sprayed onto the inner wall of the culture bottle from multiple nozzles 31, thus spraying and rinsing the inner wall of the culture bottle with disinfectant water. After the water in the tube rack 12 is cut off, the culture bottle pushes the pin 4 and nozzle 3 up and down repeatedly. Since the nozzle 31 and the tube hole 24 are connected to the inside of the bottle, the circular plate 41 slides on the guide post 25, and a piston movement is formed inside the water storage shell 2, which sprays air from the upper port of the water storage shell 2 to act on the inner wall of the culture bottle, thus achieving the function of blowing out a certain amount of air to evaporate water vapor.

[0055] The above description only illustrates certain exemplary embodiments of the present invention. Undoubtedly, those skilled in the art can modify the described embodiments in various ways without departing from the spirit and scope of the present invention. Therefore, the above drawings and descriptions are illustrative in nature and should not be construed as limiting the scope of protection of the claims of the present invention.

Claims

1. A batch sterilization device for primary fibroblast culture medium, comprising a cleaning tank (1) equipped with a door panel (11), characterized in that, It also includes a pipe rack (12) and a water storage tank (2) connected on the pipe rack (12), wherein the water storage tank (2) is provided with a nozzle (3) that moves along a vertical moving path and has a nozzle (31) on its side wall. The water storage shell (2) is also fixedly installed with a plurality of shell plates (21) arranged perpendicular to its side wall, and the end of the shell plate (21) is fixedly installed with an elastic strip (22) located outside the nozzle (3).

2. The batch sterilization device for primary fibroblast culture medium according to claim 1, characterized in that, The water storage shell (2) is connected to a vertical pipe (23) that slides inside the nozzle (3).

3. The batch sterilization device for primary fibroblast culture medium according to claim 2, characterized in that, The vertical pipe (23) has multiple pipe holes (24) that are connected to the nozzle (31).

4. The batch sterilization device for primary fibroblast culture medium according to claim 3, characterized in that, The nozzle (3) has a slider (32) fixedly installed at its lower port, which slides in the pipe hole (24).

5. The batch sterilization device for primary fibroblast culture medium according to claim 3, characterized in that, The nozzle (3) and the water storage shell (2) are fixedly installed with springs located inside the vertical pipe (23).

6. The batch sterilization device for primary fibroblast culture medium according to claim 3, characterized in that, It also includes a circular plate (41) that slides in the water storage tank (2) and is used to open and close the upper port of the water storage tank (2).

7. The batch sterilization device for primary fibroblast culture medium according to claim 6, characterized in that, The circular plate (41) has multiple openings (42), and the water storage shell (2) has a guide post (25) that is inserted into the openings (42).

8. The batch sterilization device for primary fibroblast culture medium according to claim 7, characterized in that, A pin (4) that cooperates with the nozzle (3) is fixedly installed on the circular plate (41).

9. The batch sterilization device for primary fibroblast culture medium according to claim 7, characterized in that, The elastic strip (22) is specifically an elastic metal sheet.

10. The batch sterilization device for primary fibroblast culture medium according to claim 7, characterized in that, The circular plate (41) is specifically a stainless steel metal plate.

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