Sample pre-treatment device

Abstract

The utility model discloses a kind of sample pretreatment devices, including cabinet, operating platform installed in cabinet, sample processing module being located on operating platform and mechanical arm mechanism being installed on operating platform and / or in cabinet, mechanical arm mechanism is installed with pipette assembly and the clamping cover component being located in one side of pipette assembly, mechanical arm mechanism is used to drive pipette assembly and clamping cover component to move to the different position of sample processing module.Pipette assembly includes the pipette of being used to hold pipette gun, clamping cover component includes the first holding member for holding test tube or tube cover, and the gland piece for pressing tube cover to prevent tube cover from being skew is located in first holding member and is used to press tube cover.The mechanical arm mechanism of the sample pretreatment device integrates pipette assembly and clamping cover component, degree of automation is high, and the levelness positioning can be carried out to tube cover, prevent tube cover from being skew when screwing, improve the efficiency and security of sample pretreatment.

Description

Technical Field

[0001] This utility model relates to the field of detection and analysis technology, and in particular, to a sample pretreatment device. Background Technology

[0002] In biochemical detection, sample pretreatment is usually required before analysis to bring the sample to a state suitable for the final analysis or detection. This process is generally called sample pretreatment. Sample pretreatment typically includes various steps such as dilution, centrifugation, shaking, enrichment, and filtration. Experimenters need to follow the experimental procedures in the corresponding standard methods to carry out sample pretreatment.

[0003] Currently, these pretreatment steps typically require laboratory personnel to transport the samples to processing equipment located at different points in the laboratory, manually set the methods and parameters of the equipment, place the samples into the equipment, and perform the pretreatment operations. Some pretreatment processes require complete manual operation. For example, for the detection of viruses in water, it is usually necessary to first enrich and concentrate the sewage virus samples to extract the virus samples. However, existing pretreatment devices for sewage virus samples can only complete part of the sewage virus enrichment operations (such as centrifugation and shaking), and some operations still need to be performed manually. The low level of automation leads to high workload for testing personnel, low processing efficiency, and difficulty in meeting the rapid detection needs of large numbers of samples in sudden outbreaks. Moreover, after completing the sample pretreatment, PCR technology is generally used to perform batch testing of samples (96 samples or even more), which places high demands on the efficiency and accuracy of sewage virus sample pretreatment operations.

[0004] Chinese Patent Publication No. CN221644900U discloses a device for enriching and concentrating viruses in wastewater. The device includes an operating table with a placement area, a centrifugation area, a temporary storage area, a pipetting area, a weighing area, a mixing area, and a recovery area. The placement and temporary storage areas are arranged side-by-side on the front right side of the operating table; the weighing, pipetting, and centrifugation areas are arranged side-by-side on the rear right side of the operating table; the recovery area is located between the weighing and temporary storage areas; and the mixing area is located on the rear left side of the operating table. The pipetting area is equipped with a pipette, which can remove the supernatant from the centrifuge tube in the pipetting area, thus concentrating the sample suspension containing the virus to be tested. This patent enables high-throughput, fully automated enrichment and concentration of wastewater virus samples, effectively improving the pretreatment efficiency of wastewater virus samples. However, the air inside the equipment carries viruses, resulting in unclean air being released outside. Furthermore, the test tubes come into direct contact with the water bath reagent in the mixing area, causing contamination and cross-contamination between tubes. Water residue on the outer wall of the test tubes upon removal also affects their usability. In existing robotic arms, the grippers struggle to horizontally position the tube caps during opening and closing operations, leading to cap tilting during the process. Additionally, liquid leakage during pipetting contaminates the liquid handling area on the worktable. Moreover, the existing cap-opening / closing assembly places different caps on the same platform, causing cross-contamination and affecting the accuracy and safety of sample pretreatment. Utility Model Content

[0005] This invention provides a sample pretreatment device to solve the technical problems of low accuracy and low safety in existing sample pretreatment devices.

[0006] According to one aspect of the present invention, a sample pretreatment device is provided, including a chassis, an operating table installed inside the chassis, a sample processing module disposed on the operating table, and a robotic arm mechanism movably disposed above the sample processing module and installed on the operating table and / or inside the chassis.

[0007] The robotic arm mechanism is equipped with a pipetting assembly and a clamping assembly. The pipetting assembly is located on one side of the clamping assembly. The robotic arm mechanism is used to drive the pipetting assembly and the clamping assembly to different positions on the sample processing module.

[0008] The pipetting assembly includes a pipetting element for holding a pipette, and the capping assembly includes a first clamping element for holding a test tube and / or a capping element disposed within the first clamping element for pressing the cap to prevent the cap from tilting.

[0009] Furthermore, the pipetting assembly also includes a receiving component located on one side of the pipetting element. The receiving component includes a mounting bracket fixed to the robotic arm mechanism, a receiving box slidably mounted on the mounting bracket, and a drive component fixed to the bottom of the mounting bracket.

[0010] The output end of the drive unit is connected to the receiving box and is used to drive the receiving box to slide out below the pipette to catch the liquid dripping from the pipette.

[0011] Furthermore, the pressure cap includes a positioning plate mounted on the first clamping member, a guide cylinder disposed in the first clamping member and fixed on the positioning plate, a guide rod slidably mounted in the guide cylinder along the axial direction, an elastic member disposed in the guide cylinder and abutting against the guide rod, and a pressure cap plate mounted on the end of the guide rod away from the elastic member.

[0012] The pressure plate is horizontally positioned, and its outer diameter is less than or equal to that of the pipe cap. The pressure plate is used to press against the pipe cap under the elastic force of the elastic element.

[0013] Furthermore, the sample processing module includes a mixing module for bathing and shaking the test tube in a constant temperature water bath. The mixing module includes a water bath with an inner cavity, a temperature control component connected to the water bath for constant temperature control of the water bath, and a shaking component installed below the water bath for shaking the liquid in the test tube.

[0014] The water bath is equipped with several sleeves with their openings facing upwards for placing test tubes. The inner cavity of the water bath is used to inject a heat-conducting agent. The sleeves are inserted into the inner cavity and sealed to the end face of the water bath. The sleeves are used to insert test tubes.

[0015] Furthermore, the temperature control assembly includes a cooling pipe disposed in the inner cavity of the water bath and used to contact the heat transfer agent, and a cooling supply component for introducing coolant into the cooling pipe to adjust the temperature of the heat transfer agent, the cooling pipe being arranged around the outer periphery of the sleeve;

[0016] The water bath has a coolant inlet connected to the first end of the cooling pipe and a coolant outlet connected to the second end of the cooling pipe, with the cooling component connected to the coolant inlet.

[0017] Furthermore, the sample processing module also includes a switch cap module for cooperating with the robotic arm mechanism to screw and place the tube cap. The switch cap module includes a second clamping member for clamping the test tube and a tube cap placement member for elastically clamping the tube cap and suspending the tube cap in the air. The tube cap placement member is located on one side of the second clamping member.

[0018] Furthermore, the pipe cap placement component includes a pipe cap placement platform and a clamping spring installed on the outer peripheral wall of the pipe cap placement platform. Multiple clamping springs are spaced apart along the circumference of the pipe cap placement platform. The multiple clamping springs are used to enclose and form a clamping space that elastically clamps the pipe cap and suspends the pipe cap above the pipe cap placement platform.

[0019] Furthermore, the tube cap placement component also includes a bracket installed at the bottom of the tube cap placement platform, the bracket being used to support the tube cap placement platform at a height adapted to the test tube on the second clamping component.

[0020] Furthermore, the sample processing module also includes a pipette tip placement module for placing pipette tips or pipette tips. The pipette tip placement module includes a pipette tip box for storing pipette tips or pipette tips, a guide rail fixed to the operating table, a placement plate slidably mounted on the guide rail for placing the pipette tip box, and a pull-out plate connected to the placement plate.

[0021] The chassis has a pull-out opening for the slide-out panel to be inserted and removed.

[0022] Furthermore, a negative pressure mechanism is provided above the sample processing module for purifying and discharging the air inside the chassis. The negative pressure mechanism includes a negative pressure box installed on the chassis, a negative pressure fan installed inside the negative pressure box, a filter screen installed at one end of the negative pressure box near the inside of the chassis, and an air outlet screen installed at one end of the negative pressure box near the outside of the chassis.

[0023] Both the filter and the air outlet are connected to the negative pressure fan.

[0024] This utility model has the following beneficial effects:

[0025] The sample pretreatment device of this utility model integrates a pipetting assembly and a capping assembly into a robotic arm mechanism. The pipetting assembly, in cooperation with the robotic arm mechanism, can clamp and transfer a pipette carrying liquid. The capping assembly, in cooperation with the robotic arm mechanism, can clamp and screw the cap, and can also position the cap horizontally to prevent the cap from tilting and avoid damage to the test tube during the screwing process, effectively improving the efficiency and safety of sample pretreatment.

[0026] In addition to the objectives, features, and advantages described above, this utility model has other objectives, features, and advantages. The present utility model will now be described in further detail with reference to the figures. Attached Figure Description

[0027] The accompanying drawings, which form part of this application, are used to provide a further understanding of the present invention. The illustrative embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute an undue limitation of the present invention. In the drawings:

[0028] Figure 1 This is a schematic diagram of the mechanical arm mechanism of the sample pretreatment device according to a preferred embodiment of the present invention;

[0029] Figure 2 This is a schematic diagram of the liquid receiving component of the sample pretreatment device according to a preferred embodiment of the present invention;

[0030] Figure 3 This is a schematic diagram of the clamping cap assembly of the sample pretreatment device according to a preferred embodiment of the present invention;

[0031] Figure 4 This is a cross-sectional view of the mixing module of the sample pretreatment device according to a preferred embodiment of the present invention;

[0032] Figure 5 This is a schematic diagram of the switch cover module of the sample pretreatment device according to a preferred embodiment of the present invention;

[0033] Figure 6 This is a schematic diagram of the nozzle placement module of the sample pretreatment device according to a preferred embodiment of the present invention;

[0034] Figure 7 This is a schematic diagram of the negative pressure mechanism of the sample pretreatment device according to a preferred embodiment of the present invention.

[0035] Legend:

[0036] 100. Negative pressure mechanism; 101. Negative pressure box; 102. Negative pressure fan; 200. Mixing module; 201. Water bath; 2011. Sleeve; 2012. Thermal conductive agent; 202. Temperature control component; 2021. Cooling pipe; 2022. Temperature sensor; 203. Oscillation component; 300. Cover switch module; 301. Second clamping component; 3011. Second gripper; 302. Pipe cap placement component; 3021. Pipe cap placement platform; 3022. Cover spring; 400. Robotic arm mechanism; 500 501. Pipette assembly; 502. Receiving component; 5021. Fixing bracket; 5022. Receiving box; 5023. Driving component; 600. Clamping cap assembly; 601. First clamping component; 6011. Fixing base; 6012. First gripper; 602. Capping component; 6021. Positioning plate; 6022. Guide cylinder; 6023. Guide rod; 6024. Capping plate; 700. Pipe tip placement module; 701. Pipe tip box; 702. Guide rail; 703. Placement plate; 704. Pull-out plate. Detailed Implementation

[0037] The embodiments of the present invention will be described in detail below with reference to the accompanying drawings. However, the present invention can be implemented in many different ways as defined and covered below.

[0038] like Figures 1 to 7As shown, the sample pretreatment device of this embodiment is used for concentrating and enriching sewage viruses. The device includes a chassis, an operating table installed inside the chassis, a sample processing module mounted on the operating table, and a robotic arm mechanism 400 movably mounted above the sample processing module and installed on the operating table and / or inside the chassis. Specifically, the sample processing module includes a mixing module 200, a centrifugation module, a weighing module, a recovery module, a cap-opening module 300, and a pipette tip placement module 700. By integrating mixing, centrifugation, weighing, cap-opening, and pipetting modules, the entire process of sewage virus enrichment is automated, significantly improving processing efficiency.

[0039] like Figure 1 As shown, a pipetting assembly 500 and a capping assembly 600 are mounted on the robotic arm mechanism 400. The pipetting assembly 500 is located on one side of the capping assembly 600. The robotic arm mechanism 400 is used to drive the pipetting assembly 500 and the capping assembly 600 to different positions on the sample processing module. The pipetting assembly 500 includes a pipette 501, which is mounted on the robotic arm mechanism 500 and used to hold the pipette. Thus, the pipette containing liquid can move along with the robotic arm mechanism 400 on the pipette 501 to transfer the liquid to a designated location. The capping assembly 600 includes a first clamping member 601 for clamping the test tube and / or the cap, and a capping member 602 disposed within the first clamping member 601 for pressing the cap to prevent it from tilting. The robotic arm mechanism 400 integrates the pipetting assembly 500 and the capping assembly 600, so that the pipetting assembly 500 can clamp and transfer the pipette carrying liquid by cooperating with the robotic arm mechanism 400, and the capping assembly 600 can clamp and screw the cap by cooperating with the robotic arm mechanism 400, and can position the cap horizontally to prevent it from tilting, avoid damage to the test tube during the screwing process, and improve processing efficiency.

[0040] like Figure 1 and Figure 2As shown, the pipetting assembly 500 also includes a receiving component 502 located below the pipetting component 501. The receiving component 502 includes a fixing frame 5021, a receiving box 5022, and a driving component 5023. Specifically, the fixing frame 5021 includes a horizontal plate and a vertical plate and a guide plate connected to the upper and lower ends of the horizontal plate. The horizontal plate and the vertical plate are fixed on the robotic arm mechanism 400. The guide plate and the driving component 5023 are located on the same side of the horizontal plate. The guide plate has a through hole for the output end of the driving component 5023 to pass through, and the guide plate has grooves on both sides. The receiving box 5022 is placed on the guide plate and slides through the grooves. The output end of the driving component 5023 is connected to the receiving box 5022. Therefore, after the pipette 501 completes the liquid dispensing process, the drive unit 5023 can control the receiving box 5022 to extend below the pipette 501 to catch the liquid dripping from the pipette, preventing leakage during the pipetting process and avoiding cross-contamination. Preferably, the drive unit 5023 is a drive motor. Preferably, the receiving box 5022 has a groove to receive the liquid dripping from the pipette and prevent the liquid from falling off the receiving box 5022.

[0041] like Figure 1 and Figure 3As shown, optionally, the first clamping member 601 includes a fixed base 6011 mounted on the robotic arm mechanism 400 and a first gripper 6012 disposed below the fixed base 6011. The first gripper 6012 is radially movably mounted on the fixed base 6011 to clamp the test tube and / or the tube cap. A capping member 602 is disposed within the first gripper 6012 to press the tube cap to keep the upper end of the tube cap horizontal and prevent the tube cap from tilting during the capping process. The capping member 602 includes a positioning plate 6021, a guide cylinder 6022, a guide rod 6023, an elastic element, and a capping plate 6024. Optionally, the elastic element is a spring. Specifically, the positioning plate 6021 is installed on the fixed base 6011, the guide cylinder 6022 is located inside the first gripper 6012 and fixed on the positioning plate 6021, one end of the guide rod 6023 is axially slidably installed inside the guide cylinder 6022, and the other end extends out of the guide cylinder 6022 and is fixed to the cover plate 6024, the elastic element is located inside the guide cylinder 6022 and abuts against the end of the guide rod 6023, a limiting ring is provided at the end of the guide cylinder 6022 near the cover plate 6024, and a limiting block is provided at the end of the guide rod 6023 away from the cover plate 6024. Through the cooperation of the limiting ring and the limiting block, the guide rod 6023 can be prevented from coming out of the guide cylinder 6022. The pressure plate 6024 is horizontally positioned within the first clamping member 601. The outer diameter of the pressure plate 6024 is less than or equal to the outer diameter of the tube cap or test tube, ensuring that the first gripper 6012 can firmly clamp the tube cap or test tube. The pressure plate 6024 is used to press against the tube cap under the elastic force of the elastic element. Thus, when the first clamping member 601 moves with the robotic arm mechanism 400 to above the tube cap to be retrieved, the first gripper 6012 is controlled to open and move towards the tube cap. At this time, the pressure plate 6024 presses against the tube cap under the force of the elastic element, keeping the upper end of the tube cap horizontal. Then, the first gripper 6012 is tightened to clamp the tube cap for twisting or transferring, effectively preventing the tube cap from tilting.

[0042] Preferably, the first clamping member 601 is provided with a pressure detection element, which is used to contact the pipe cap to detect the pressure value of the pipe cap at a preset position, thereby determining whether the pipe cap is properly closed.

[0043] like Figure 4As shown, the mixing module 200 is used to bathe and vibrate the test tubes in a constant-temperature water bath. The mixing module 200 includes a water bath 201, a temperature control component 202 connected to the water bath 201 for maintaining a constant temperature in the water bath 201, and a vibration component 203 installed below the water bath 201 for vibrating the liquid inside the test tubes. The water bath 201 includes a water bath 201 with an inner cavity, and several sleeves 2011 with upward-facing openings for placing the test tubes. The inner cavity is used to inject a heat-conducting agent 2012. The sleeves 2011 pass through the inner cavity and are sealed to the end face of the water bath 201. The sleeves 2011 are used to insert the test tubes. Thus, through the cooperation of the water bath 201 and the temperature control component 202, the mixing module 200 can precisely adjust and stabilize the temperature inside the water bath 201, keeping the test tubes placed in the sleeves 2011 at a constant temperature to meet the precise temperature requirements of different experiments. By placing the test tube inside the sleeve 2011 and ensuring the sleeve 2011 is in contact with the heat transfer agent 2012, not only can the temperature of the heat transfer agent 2012 be rapidly conducted to the test tube through the sleeve 2011 for precise temperature control, but the test tube and the heat transfer agent 2012 are also isolated, preventing leakage of the heat transfer agent 2012 to other areas of the operating table during oscillation and avoiding contamination. This also prevents contamination of the heat transfer agent 2012 by the test tube and prevents cross-contamination between test tubes. Furthermore, it avoids water residue on the outer wall after removal, effectively improving the reliability and safety of the mixing module 200. In addition, designing the water bath 201 as a lidless structure eliminates the need for a flip-top, allowing direct compatibility with the robotic arm mechanism 400 for test tube loading and unloading, significantly improving processing efficiency and automation.

[0044] like Figure 4As shown, the temperature control assembly 202 includes a cooling pipe 2021 disposed within the inner cavity of the water bath 201 and used for contacting the heat transfer fluid 2012, and a cooling supply component for introducing coolant into the cooling pipe 2021 to regulate the temperature of the heat transfer fluid 2012. The cooling pipe 2021 is arranged around the outer periphery of the sleeve 2011. The water bath 201 has a coolant inlet communicating with a first end of the cooling pipe 2021 and a coolant outlet communicating with a second end of the cooling pipe 2021. The cooling supply component is connected to the coolant inlet. The temperature control assembly 202 includes a cooling pipe 2021 and a cooling supply component. Specifically, a heat-conducting agent 2012 fills the inner cavity of the water bath 201 and contacts the outer wall of the sleeve 2011 to uniformly transfer heat to the sleeve 2011. The cooling pipe 2021 is disposed in the inner cavity of the water bath 201 and contacts the heat-conducting agent 2012 to regulate the temperature of the heat-conducting agent 2012. Preferably, the cooling pipe 2021 is arranged around the outer periphery of the sleeve 2011 to ensure that the heat-conducting agent 2012 uniformly transfers temperature to the sleeve 2011 along the circumference. Preferably, the cooling pipe 2021 is made of copper or stainless steel to improve heat exchange efficiency. The water bath 201 has a coolant inlet and a coolant outlet. The coolant inlet is connected to the first end of the cooling pipe 2021, and the coolant outlet is connected to the second end of the cooling pipe 2021. A cooling supply component is connected to the coolant inlet to introduce coolant into the cooling pipe 2021 and provide a certain amount of circulation power. Preferably, the coolant temperature is controlled at around 1-3℃. Preferably, the cooling supply component is a circulating water pump. In use, the circulating water pump is turned on, and the coolant enters from the coolant inlet, exits through the cooling pipe 2021 from the coolant outlet, and returns to the circulating water pump for cooling. The cooling pipe 2021 is coiled inside the water bath 201 to increase the contact area with the heat transfer agent 2012, thereby removing heat from the heat transfer agent 2012 and lowering its temperature. This achieves temperature regulation of the water bath 201's interior, thus maintaining a constant temperature for the test tubes. Preferably, in this embodiment, the coolant inlet and coolant outlet are located on the same side of the water bath 201, and there are at least two coolant inlets and outlets to improve cooling circulation efficiency. The temperature of the heat transfer fluid 2012 is controlled via the cooling pipe 2021, thereby indirectly controlling the temperature of the test tube, resulting in better temperature stability and constant temperature control. Simultaneously, the airtightness requirements of the water bath 201 are lower, simplifying the structural design.

[0045] like Figure 4As shown, the temperature control component 202 also includes a temperature sensor 2022, which is installed on the water bath 201 and communicates with the inner cavity of the water bath 201 to monitor the temperature of the heat transfer agent 2012. Preferably, the temperature sensor 2022 and the cooling component are electrically connected to the control system. The control system can receive the real-time measurement value of the temperature sensor 2022 and adjust the flow rate of the cooling component accordingly to ensure temperature stability. Preferably, the temperature sensor 2022 controls the temperature of the heat transfer agent 2012 at 4±1℃. The internal temperature of the sleeve 2011 immersed in the heat transfer agent 2012 changes with the temperature of the heat transfer agent 2012. After the test tube is placed in the sleeve 2011 and stabilized for a period of time, the temperature of the liquid in the test tube can be stabilized at 4±1℃. Preferably, the temperature control component 202 also includes a heat insulation layer disposed in the inner cavity of the water bath 201. The heat insulation layer is installed on the inner wall of the water bath 201 to prevent the heat transfer agent 2012 from escaping, reduce heat loss, and improve energy efficiency.

[0046] like Figure 4 As shown, the oscillation mechanism includes a rotary platform, an eccentric shaft assembly, and a base arranged sequentially from top to bottom, as well as a power component installed within the base. The rotary platform is located below and fixed to the water bath 201. The eccentric shaft assembly includes several eccentric shafts eccentrically positioned at the lower end of the rotary platform. The power component is connected to the eccentric shafts via a transmission wheel set. The power component drives the rotary platform to oscillate the water bath 201 and stops the oscillation at a set position. Through the synergistic effect of the water bath 201 and the oscillation mechanism, the test tubes can be uniformly oscillated under constant temperature conditions, simplifying the operation process, reducing manual intervention, and improving the degree of automation.

[0047] like Figure 5 As shown, the cap-screwing module 300 is used in conjunction with the robotic arm mechanism 400 to screw and place the caps of test tubes. The cap-screwing module 300 includes a second clamping member 301 for holding the test tubes, and a cap-placement member 302 for elastically clamping the caps and suspending them in mid-air. The cap-placement member 302 is located on one side of the second clamping member 301. Thus, the caps are suspended and positioned by the elastic clamping of the caps by the cap-placement member 302, effectively preventing cross-contamination between caps and facilitating the gripping operation of the robotic arm mechanism 400.

[0048] Specifically, the second clamping member 301 includes a second gripper 3011 for clamping test tubes. In use, the robotic arm mechanism 400 can grasp the test tube with the cap tightly closed via the cap clamping assembly 600, place the test tube within the space enclosed by the second gripper 3011 in its open state, and then control the second gripper 3011 to tighten, thereby achieving a stable clamping of the test tube. Then, the cap clamping assembly 600 on the robotic arm mechanism 400 positions and clamps the cap of the test tube within the second gripper 3011. The robotic arm mechanism 400 can then rotate the cap to perform opening or closing operations. After the opened cap is transferred to the cap placement member 302 by the robotic arm mechanism 400, the cap clamping assembly 600 is released to temporarily place the cap. Preferably, there are two or more second clamping members 301 to improve processing efficiency.

[0049] like Figure 5 As shown, a cap placement member 302 is disposed on one side of the second clamping member 301. The cap placement member 302 includes a cap placement platform 3021 and cap clamping springs 3022 installed on the outer peripheral wall of the cap placement platform 3021. Multiple cap clamping springs 3022 are spaced apart along the circumference of the cap placement platform 3021. These multiple cap clamping springs 3022 are used to enclose and form a clamping space that elastically clamps the cap and suspends the cap above the cap placement platform 3021. Preferably, the shape of the cap placement platform 3021 is adapted to the shape of the cap. Preferably, the upper end of the cap clamping spring 3022 extends radially outward away from the clamping space, so that the upper port diameter of the clamping space is larger than the outer diameter of the cap, thereby guiding the cap and preventing it from being affected when it enters the clamping space. Preferably, there are two or more cap placement members 302. In use, the tube cap is held by the cap clamping assembly 600 and transferred to the tube cap placement platform 3021 by the robotic arm mechanism 400. The tube cap is then pressed down into the clamping space. The cap clamping spring 3022 clamps the tube cap at a certain height in the clamping space through elastic force, thereby suspending the tube cap above the tube cap placement platform 3021, avoiding contact between the tube cap and the tube cap placement platform 3021, and preventing cross-contamination between tube caps.

[0050] like Figure 5 As shown, the cap placement component 302 also includes a bracket mounted on the bottom of the cap placement platform 3021. The bracket supports the cap placement platform 3021 to a height adapted to the test tube on the second clamping member 301, so that the cap clamping assembly 600 on the robotic arm mechanism 400 can grasp the cap. Preferably, the cap opening and closing assembly also includes a support plate connected to the operating table, the support plate being mounted on the bottom of the second clamping member 301 and the cap placement component 302.

[0051] like Figure 6As shown, the pipette tip placement module 700 includes a pipette tip box 701, a guide rail 702, a placement plate 703, and a pull-out plate 704. The pipette tip box 701 is used to store pipette tips and / or pipette tips. The placement plate 703 is used to place the pipette tip box 701. The guide rail 702 is fixed to the operating table and located on both sides of the placement plate 703. The placement plate 703 is slidably mounted on the guide rail 702. The pull-out plate 704 is located at the end of the placement plate 703 away from the housing and is connected to the placement plate 703. The housing has a pull-out opening for the pull-out plate 704 to enter and exit. Preferably, the pipette tip box 701 is detachably fixed to the placement plate 703. By connecting the pull-out plate 704, the placement plate 703, and the guide rail 702 to form a drawer-type structure, operators can easily pull out the pull-out plate 704 from outside the chassis to replace the pipette tip box 701 and / or pipettes, ensuring sufficient pipette tips or nozzles are available. Preferably, the end of the guide rail 702 away from the pull-out plate 704 is equipped with a locking element. This serves two purposes: firstly, it locks the position of the placement plate 703, preventing it from shifting during the docking of the pipette with the tip or nozzle, ensuring accurate assembly of the tip or nozzle onto the pipette; secondly, it accurately positions the placement plate 703, ensuring it reaches the designated position after each replacement of the pipette tip box 701. Preferably, the locking element is an electromagnetic lock.

[0052] like Figure 7 As shown, a negative pressure mechanism 100 is installed above the sample processing module. The negative pressure mechanism 100 is mounted on the chassis and connected to the outside. The negative pressure mechanism 100 purifies the air inside the chassis before discharging it, thus purifying the air inside the chassis, preventing the leakage of viral aerosols, ensuring a clean external environment, and protecting the safety of operators. Preferably, an ultraviolet lamp is also installed inside the chassis. Before liquid processing, the ultraviolet lamp can be turned on to disinfect the chassis and sample processing module, ensuring the accuracy of wastewater virus enrichment.

[0053] Specifically, the negative pressure mechanism 100 includes a negative pressure chamber 101, a negative pressure fan 102 installed inside the negative pressure chamber 101, a filter screen installed at one end of the negative pressure chamber 101 near the inside of the chamber, and an air outlet screen installed at one end of the negative pressure chamber 101 near the outside of the chamber. Both the filter screen and the air outlet screen are connected to the negative pressure fan 102. In use, the negative pressure fan 102 is turned on to create a negative pressure environment inside the negative pressure chamber 101, allowing air from inside the chamber to enter the chamber after being purified by the filter screen, and then discharged to the outside of the chamber through the air outlet screen. This prevents viruses from being released from inside the chamber, thus preventing contamination of the inside and outside of the chamber and ensuring the accuracy of sample processing and the safety of external personnel. Preferably, a dust filter is also installed on the air outlet screen to prevent external dust from entering the chamber, ensuring the cleanliness of the inside of the chamber and avoiding affecting the accuracy of liquid processing.

[0054] The above description is merely a preferred embodiment of this utility model and is not intended to limit the utility model. Various modifications and variations can be made to this utility model by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this utility model should be included within the protection scope of this utility model.

Claims

1. A sample pretreatment device, comprising a chassis, an operating table installed within the chassis, a sample processing module disposed on the operating table, and a robotic arm mechanism (400) movably disposed above the sample processing module and installed on the operating table and / or within the chassis, characterized in that, The robotic arm mechanism (400) is equipped with a pipetting assembly (500) and a capping assembly (600). The pipetting assembly (500) is located on one side of the capping assembly (600). The robotic arm mechanism (400) is used to drive the pipetting assembly (500) and the capping assembly (600) to move to different positions on the sample processing module. The pipetting assembly (500) includes a pipetting element (501) for holding a pipette, and the capping assembly (600) includes a first clamping element (601) for holding a test tube and / or a cap, and a capping element (602) disposed in the first clamping element (601) for pressing the cap to prevent the cap from tilting.

2. The sample pretreatment apparatus according to claim 1, characterized in that, The pipetting assembly (500) further includes a receiving component (502) disposed on one side of the pipetting element (501). The receiving component (502) includes a fixing frame (5021) fixed on the robotic arm mechanism (400), a receiving box (5022) slidably mounted on the fixing frame (5021), and a driving component (5023) fixed to the bottom of the fixing frame (5021). The output end of the drive unit (5023) is connected to the receiving box (5022) and is used to drive the receiving box (5022) to extend below the pipette (501) to catch the liquid dripping from the pipette.

3. The sample pretreatment apparatus according to claim 1, characterized in that, The pressure cap (602) includes a positioning plate (6021) mounted on the first clamping member (601), a guide cylinder (6022) disposed in the first clamping member (601) and fixed on the positioning plate (6021), a guide rod (6023) slidably mounted in the guide cylinder (6022) along the axial direction, an elastic member disposed in the guide cylinder (6022) and abutting against the guide rod (6023), and a pressure cap (6024) mounted on the end of the guide rod (6023) away from the elastic member; The pressure plate (6024) is horizontally arranged, and the outer diameter of the pressure plate (6024) is less than or equal to the outer diameter of the pipe cap. The pressure plate (6024) is used to press against the pipe cap under the elastic force of the elastic member.

4. The sample pretreatment apparatus according to claim 1, characterized in that, The sample processing module includes a mixing module (200) for constant temperature water bath and agitation of the test tube. The mixing module (200) includes a water bath (201) with an inner cavity, a temperature control component (202) connected to the water bath (201) for constant temperature control of the water bath (201), and an agitation component (203) installed below the water bath (201) for agitation of the liquid in the test tube. The water bath (201) is equipped with several sleeves (2011) with their openings facing upwards for placing the test tubes. The inner cavity of the water bath (201) is used to inject a heat-conducting agent (2012). The sleeves (2011) are inserted into the inner cavity and are sealed to the end face of the water bath (201). The sleeves (2011) are used to insert the test tubes.

5. The sample pretreatment apparatus according to claim 4, characterized in that, The temperature control assembly (202) includes a cooling pipe (2021) disposed in the inner cavity of the water bath (201) and used to contact the heat transfer agent (2012), and a cooling supply component for introducing coolant into the cooling pipe (2021) to adjust the temperature of the heat transfer agent (2012). The cooling pipe (2021) is arranged around the outer periphery of the sleeve (2011). The water bath (201) has a coolant inlet connected to the first end of the cooling pipe (2021) and a coolant outlet connected to the second end of the cooling pipe (2021), and the cooling supply component is connected to the coolant inlet.

6. The sample pretreatment apparatus according to claim 1, characterized in that, The sample processing module further includes a switch cap module (300) for cooperating with the robotic arm mechanism (400) to screw and place the tube cap. The switch cap module (300) includes a second clamping member (301) for clamping the test tube, and a tube cap placement member (302) for elastically clamping the tube cap and suspending the tube cap. The tube cap placement member (302) is located on one side of the second clamping member (301).

7. The sample pretreatment apparatus according to claim 6, characterized in that, The pipe cap placement component (302) includes a pipe cap placement platform (3021) and a clamping spring (3022) installed on the outer peripheral wall of the pipe cap placement platform (3021). Multiple clamping springs (3022) are provided at intervals along the circumference of the pipe cap placement platform (3021). The multiple clamping springs (3022) are used to enclose and form a clamping space that elastically clamps the pipe cap and suspends the pipe cap above the pipe cap placement platform (3021).

8. The sample pretreatment apparatus according to claim 7, characterized in that, The tube cap placement component (302) further includes a bracket installed at the bottom of the tube cap placement platform (3021), the bracket being used to support the tube cap placement platform (3021) at a height adapted to the test tube on the second clamping component (301).

9. The sample pretreatment apparatus according to claim 1, characterized in that, The sample processing module further includes a pipette tip placement module (700) for placing the pipette tip or pipette tip. The pipette tip placement module (700) includes a pipette tip box (701) for storing the pipette tip or pipette tip, a guide rail (702) fixed to the operating table, a placement plate (703) slidably mounted on the guide rail (702) for placing the pipette tip box (701), and a pull-out plate (704) connected to the placement plate (703). The chassis has a pull-out opening for the pull-out plate (704) to enter and exit.

10. The sample pretreatment apparatus according to claim 1, characterized in that, The sample processing module is provided with a negative pressure mechanism (100) for purifying and discharging the air inside the chassis. The negative pressure mechanism (100) includes a negative pressure box (101) installed on the chassis, a negative pressure fan (102) installed inside the negative pressure box (101), a filter screen installed at one end of the negative pressure box (101) near the inside of the chassis, and an air outlet screen installed at one end of the negative pressure box (101) near the outside of the chassis. Both the filter and the air outlet are connected to the negative pressure fan (102).

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