Controllable particle size gun head for extracting silk fibroin nanoparticles
By designing a controllable particle size nozzle and combining it with crushing and filtering structures, the problems of long preparation time and non-uniform particle size in traditional methods have been solved. This has enabled the rapid and precise preparation of silk fibroin nanoparticles of a specified size, improving preparation efficiency and particle size uniformity.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- SUZHOU UNIV
- Filing Date
- 2025-06-16
- Publication Date
- 2026-06-30
AI Technical Summary
Existing technologies for preparing silk fibroin nanoparticles are time-consuming, difficult to precisely control particle size, and have inconsistent particle sizes. Traditional pipettes have simple structures and cannot meet the requirements of demanding application scenarios.
Design a controllable particle size nozzle that combines a crushing structure and a filtration structure. It directly crushes and sieves silk fibroin particles during liquid discharge using a pipette. Particle size control is achieved through a particle cutting mesh and a microporous filter membrane. The nozzle body adopts an umbrella-shaped structure to expand the filtration area, and the connecting tube uses a rubber hose for easy disassembly and connection.
It enables rapid and precise preparation of silk fibroin nanoparticles of specified sizes, improving preparation efficiency, ensuring particle dispersion and uniformity, and facilitating cleaning and various experimental needs.
Smart Images
Figure CN224422922U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of nanoparticle preparation technology, specifically to a controllable particle size extraction nozzle for extracting silk fibroin nanoparticles. Background Technology
[0002] The preparation of silk fibroin nanoparticles usually employs ultrasonic treatment. The core principle of ultrasonic treatment for preparing silk fibroin nanoparticles is to utilize the cavitation effect of ultrasound. When ultrasound propagates in a liquid medium, it generates high-frequency mechanical vibrations, causing the formation of tiny bubbles (cavitation bubbles) between liquid molecules. When the cavitation bubbles rapidly collapse, they generate instantaneous high temperatures (approximately 5000K), high pressures (approximately 100MPa), and strong shock waves in a localized manner, prompting silk fibroin molecules to self-assemble or break into nanoscale particles.
[0003] A pipette is a precision tool commonly used in laboratories for transferring liquids. It uses the axial movement of a piston within a chamber to change the internal volume, thereby creating a pressure difference to achieve the aspiration or dispensing of liquids.
[0004] However, traditional sound processing methods suffer from problems such as long preparation time, difficulty in accurately controlling the particle size of nanoparticles, and uneven size of the prepared nanoparticles, resulting in unstable product quality and failing to meet the requirements of some application scenarios with high requirements for particle size uniformity.
[0005] Moreover, existing pipettes have relatively simple structural designs. Therefore, there is an urgent need for a new device to work with pipettes to solve the above problems and achieve rapid and precise preparation of silk fibroin nanoparticles. Utility Model Content
[0006] The purpose of this invention is to overcome the shortcomings of the existing technology and provide a controllable particle size pipette tip for extracting silk fibroin nanoparticles. It does not require long-term ultrasonic treatment, directly breaks up silk fibroin particles during pipetting, and can accurately control the particle size of silk fibroin nanoparticles to obtain silk fibroin nanoparticles of a specified size.
[0007] To achieve the above objectives, the technical solution adopted by this utility model is as follows: a controllable particle size pipette head for extracting silk fibroin nanoparticles, comprising a pipette head body, a connecting tube at one end of the pipette head body, a filter structure for sieving silk fibroin molecules at the other end, a crushing structure for breaking silk fibroin molecules installed inside the pipette head body, and the pipette head body being connected to the output end of a pipette body through the connecting tube.
[0008] Optionally, the crushing structure adopts a particle cutting mesh, and the particle cutting mesh is fixedly installed in the middle of the inner side of the gun head body.
[0009] Optionally, the filtration structure employs a microporous membrane, and the pore size of the microporous membrane is 150 nm.
[0010] Optionally, the gun head body adopts an umbrella-shaped structure, and the inner diameter of the gun head body increases axially from the inlet end to the outlet end.
[0011] Optionally, a pipette tip is installed at the output end of the pipette body, and the pipette tip body is fixedly installed on the pipette tip through the connecting tube.
[0012] Optionally, the connecting tube is fixedly sleeved on the pipette tip, and the pipette tip is in communication with the tip body.
[0013] Optionally, the filter structure is located at the outlet end of the nozzle body, and the connecting pipe is located at the inlet end of the nozzle body.
[0014] Optionally, the connecting tube is a rubber hose, and the inner diameter of the rubber hose is smaller than the outer diameter of the pipette tip.
[0015] Compared with the prior art, the beneficial effects of this utility model are as follows:
[0016] (1) In this utility model, when the pipette body is discharging liquid, the silk fibroin molecules enter the pipette head of the controllable particle size pipette head, are first mechanically crushed by the crushing structure, and then the silk fibroin particles that meet the requirements are screened out by the filter structure. Compared with the traditional ultrasonic treatment method, silk fibroin nanoparticles of a specified size can be directly obtained through this controllable particle size pipette head. It is convenient to use and realizes the preparation of silk fibroin nanoparticles in small quantities and quickly, effectively improving the efficiency of preparing silk fibroin nanoparticles.
[0017] (2) In this utility model, the solution outlet port is set as an umbrella-shaped structure, which can expand the filtration area, reduce the fluid pressure, and make the filtered solution flow out in a uniformly dispersed state, further ensuring the dispersion and uniformity of particle size.
[0018] (3) In this utility model, the controllable particle size pipette tip is detachably connected to the pipette tip through the elastic deformation of the connecting tube, allowing repeated insertion and removal, facilitating quick disassembly and cleaning after the experiment, and having good sealing performance. Attached Figure Description
[0019] Figure 1 This is a schematic diagram of the controllable particle size gun head in an embodiment of this utility model;
[0020] Figure 2 This is a schematic diagram of the internal structure of the controllable particle size gun head in an embodiment of this utility model;
[0021] Figure 3This is an exploded structural diagram of the pipette body, pipette tip, and controllable particle size pipette tip in an embodiment of this utility model;
[0022] The components are: 1. Pipette body; 2. Pipette tip; 3. Controllable particle size pipette tip; 31. Pipette tip body; 32. Filter structure; 33. Crushing structure; 34. Connecting tube. Detailed Implementation
[0023] The present invention will now be described in further detail with reference to the accompanying drawings and embodiments. These drawings are simplified schematic diagrams, which are only used to illustrate the basic structure of the present invention in a schematic manner, and therefore only show the components related to the present invention.
[0024] like Figures 1-2 As shown, a controllable particle size pipette tip for extracting silk fibroin nanoparticles includes a pipette tip body 31, a filter structure 32, a crushing structure 33, and a connecting tube 34. The filter structure 32 is fixedly installed at the outlet end of the pipette tip body 31, the inlet end of the pipette tip body 31 is fixedly connected to the connecting tube 34, the crushing structure 33 is fixedly installed inside the pipette tip body 31, and the pipette tip body 31 is connected to the output end of the pipette body 1 through the connecting tube 34.
[0025] The connecting tube 34 is used to connect the pipette head body 31 and the pipette body 1. The crushing structure 33 can mechanically crush silk fibroin molecules into silk fibroin particles of a specified size, while the filtering structure 32 physically sieves the crushed silk fibroin particles, allowing only silk fibroin particles that meet the required size to be discharged from the outlet of the pipette head body 31.
[0026] When the pipette body 1 discharges liquid, silk fibroin molecules enter the pipette head 31 of the controllable particle size pipette head 3. They are first mechanically broken by the crushing structure 33, and then screened by the filtration structure 32 to discharge silk fibroin particles that meet the requirements. It is convenient to use and can realize the preparation of silk fibroin nanoparticles in small quantities and quickly, effectively improving the efficiency of silk fibroin nanoparticle preparation. Compared with the traditional ultrasonic treatment method, silk fibroin nanoparticles of a specified size can be directly obtained through the controllable particle size pipette head 3.
[0027] As described above, the nozzle body 31 adopts an umbrella-shaped structure, and the inner diameter of the nozzle body 31 increases axially from the inlet end to the outlet end. Setting the solution outlet port as an umbrella-shaped structure can expand the filtration area, reduce the fluid pressure, and allow the filtered solution to flow out in a uniformly dispersed state, further ensuring the dispersion and particle size uniformity of the particles.
[0028] The filter structure 32 uses a microporous filter membrane with a pore size of 150 nm. The particle size is further precisely controlled through the sieving action of the microporous filter membrane. At this time, particles larger than 150 nm are trapped in the nozzle body 31, while silk fibroin nanoparticles smaller than or equal to 150 nm pass through the filter membrane with the solution.
[0029] The fragmentation structure 33 consists of an extremely sharp particle cutting mesh, which is fixedly installed in the middle of the inner side of the gun head body 31. The sharp mesh edges mechanically break down silk fibroin molecules, forming preliminary nanoscale particles. The broken silk fibroin nanomolecules are then sieved through a microporous membrane to obtain silk fibroin nanoparticles of a specified size.
[0030] like Figure 3 As shown, pipette tip 2 is a standard pipette tip, connected to the output end of pipette body 1. Controllable particle size pipette tip 3 is detachably connected to pipette tip 2 via connecting tube 34, allowing for repeated insertion and removal, facilitating quick disassembly and cleaning after experiments. When extracting silk fibroin nanoparticles from a sample volume, a pipette tip of the appropriate model can be used, and the controllable particle size pipette tip 3 can be installed as needed to meet various experimental requirements.
[0031] As described above, the pipette tip body 31 is fixedly installed on the pipette tip 2 via a connecting tube 34, that is, the connecting tube 34 is fixedly sleeved on the pipette tip 2, and the pipette tip 2 is connected to the pipette tip body 31. Under the action of the pipette body 1, the solution discharged from the pipette tip 2 enters the interior of the pipette tip body 31 through the connecting tube 34, is first mechanically broken by a particle cutting mesh, and then filtered through a microporous membrane before being discharged, thereby obtaining silk fibroin nanoparticles of a specified size.
[0032] The connecting tube 34 is made of rubber tubing, the inner diameter of which is smaller than the outer diameter of the pipette tip 2. The connecting tube 34 is sleeved on the pipette tip 2, and the two are detachably connected by the elastic deformation of the rubber tubing, and have good sealing performance.
[0033] To better understand the technical solution of this utility model, the method of using this utility model is illustrated here by taking the extraction of silk fibroin nanoparticles in one step as an example:
[0034] (1) The staff first selects a pipette tip 2 (ordinary pipette tip, such as 100μL or 1mL specification) that is suitable for the experimental requirements, and firmly installs it on the output connection port of the pipette body 1 to ensure a seal without leakage;
[0035] (2) Aspirate the sample solution to be processed into the pipette tip 2, then align the connecting tube 34 of the controllable particle size pipette tip 3 with the outlet end of the pipette tip 2, rotate the connecting tube 34 slightly, and put one end of the tubing onto the pipette tip 2 (the insertion depth is about 5-8 mm). Ensure that the connecting tube 34 is aligned with the axis of the pipette tip 2 to avoid leakage during solution flow.
[0036] (3) Press the pipette to slowly press the solution in the pipette tip 2 into the controllable particle size pipette tip 3. The sample solution to be processed passes through the crushing structure 33 and the filtering structure 32 to obtain silk fibroin nanoparticles of about 150 nm. Finally, the processed solution is introduced into the sample bottle to obtain silk fibroin nanoparticles that meet the requirements.
[0037] In summary, this invention utilizes a controllable particle size pipette tip 3, which can be connected to the pipette body 1, to directly break down silk fibroin particles during the dispensing of liquid from the pipette body 1. Furthermore, it allows for precise control of the particle size of the silk fibroin nanoparticles, accurately obtaining silk fibroin nanomolecules of approximately 150 nm. This structure is convenient to use and enables the rapid preparation of small quantities of silk fibroin nanoparticles, effectively improving the efficiency of silk fibroin nanoparticle preparation.
[0038] In the description of this utility model, it should be understood that the terms "center," "longitudinal," "lateral," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," and "outer," etc., indicating orientation or positional relationships, are based on the orientation or positional relationships shown in the accompanying drawings and are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this utility model. Furthermore, the terms "first," "second," etc., are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of indicated technical features. Thus, features defined with "first," "second," etc., may explicitly or implicitly include one or more of that feature. In the description of this utility model, unless otherwise stated, "a plurality of" means two or more.
[0039] In the description of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "joining" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.
[0040] Based on the preferred embodiments of this utility model described above, those skilled in the art can make various changes and modifications without departing from the technical concept of this utility model. The technical scope of this utility model is not limited to the contents of the specification, but must be determined according to the scope of the claims.
Claims
1. A controllable particle size extraction nozzle for silk fibroin nanoparticles, characterized in that: The device includes a pipette head (31), one end of which is provided with a connecting tube (34), and the other end is equipped with a filter structure (32) for screening silk fibroin molecules. The inside of the pipette head (31) is equipped with a breaking structure (33) for breaking silk fibroin molecules, and the pipette head (31) is connected to the output end of the pipette body (1) through the connecting tube (34).
2. The controlled particle size gun head for extracting silk fibroin nanoparticles according to claim 1, characterized in that: The crushing structure (33) adopts a particle cutting mesh, and the particle cutting mesh is fixedly installed in the middle of the inner side of the gun head body (31).
3. The controlled particle size gun head for extracting silk fibroin nanoparticles according to claim 2, characterized in that: The filter structure (32) uses a microporous filter membrane, and the pore size of the microporous filter membrane is 150 nm.
4. The controlled particle size gun head for extracting silk fibroin nanoparticles according to claim 1, characterized in that: The gun head body (31) adopts an umbrella-shaped structure, and the inner diameter of the gun head body (31) increases axially from the inlet end to the outlet end.
5. The controlled particle size gun head for extracting silk fibroin nanoparticles according to claim 1, characterized in that: The output end of the pipette body (1) is equipped with a pipette tip (2), and the tip body (31) is fixedly installed on the pipette tip (2) through the connecting tube (34).
6. The controlled particle size gun head for extracting silk fibroin nanoparticles according to claim 5, characterized in that: The connecting tube (34) is fixedly sleeved on the pipette tip (2), and the pipette tip (2) is connected to the tip body (31).
7. The controlled particle size gun head for extracting silk fibroin nanoparticles according to claim 6, characterized in that: The filter structure (32) is located at the outlet end of the gun head body (31), and the connecting pipe (34) is located at the inlet end of the gun head body (31).
8. The controlled particle size gun head for extracting silk fibroin nanoparticles according to claim 7, characterized in that: The connecting tube (34) is made of rubber tubing, and the inner diameter of the rubber tubing is smaller than the outer diameter of the pipette tip (2).