A cell culture fluid separation device

Abstract

The utility model relates to cell culture solution separation equipment technical field and disclose a cell culture solution separation device, including bearing frame, bearing frame upper portion is equipped with the bearing disc, bearing disc upper portion is equipped with a plurality of test tube seat, bearing disc lower part fixed mounting has first drive motor, and first drive motor is used for driving a plurality of test tube seat synchronous rotation, and bearing frame opposite side fixed mounting has the fixed frame located bearing frame top, and fixed frame is equipped with the moving plate inside, and moving plate lower part rotatable connection has a plurality of with test tube seat adaptation's rotating pressboard, and fixed frame upper part fixed mounting has the second drive motor for driving moving plate up and down movement, in the utility model, through first drive motor can drive a plurality of test tube seat to rotate simultaneously, can carry out centrifugal separation to single or a plurality of cell culture solution test tube, improve the applicability of this cell culture solution separation equipment.

Description

Technical Field

[0001] This utility model relates to the technical field of cell culture medium separation equipment, specifically a cell culture medium separation device. Background Technology

[0002] In fields such as biopharmaceuticals, cell biology research, and clinical diagnostics, the separation and purification of cell culture media is a key step in obtaining high-purity target products (such as recombinant proteins, monoclonal antibodies, cytokines, etc.).

[0003] A search revealed a patent, CN217663943U, which discloses a cell separation device. The device includes a housing with a partition on one side of the interior. An insulation layer is installed on the top of the partition, and its outer wall is fitted against the inner wall of the housing. A groove is formed on one side of the top of the insulation layer, and a turntable is rotatably connected inside the groove. The turntable has several placement holes on its top, each oriented at an angle. A motor is installed at the bottom of the housing, with its output end penetrating the insulation layer and connected to one side of the bottom of the turntable. A heat-conducting pipe is installed on the inner side of the insulation layer near the turntable. A heater is installed on one side of the housing, with its output end penetrating the insulation layer and connected to one side of the heat-conducting pipe. This invention has a reasonable structure, is easy to operate, and has the function of controlling temperature changes.

[0004] The aforementioned patents have significant beneficial effects, but in practical application, they still have the following shortcomings:

[0005] The aforementioned comparative documents describe inserting test tubes into placement holes and using a motor-driven turntable to achieve centrifugal separation of cell culture medium. However, in reality, sometimes only one test tube needs to be centrifuged. To ensure stability during centrifugation, it is necessary to maintain balance during rotation. When centrifuging a single test tube, a test tube of appropriate weight needs to be placed at a relatively symmetrical position to improve stability during centrifugation. This makes cell culture medium separation more complicated and reduces the applicability of the cell culture medium separation equipment. Therefore, there is an urgent need in the art to improve the cell culture medium separation device to overcome the shortcomings of the prior art. Utility Model Content

[0006] To address the shortcomings of existing technologies, this invention provides a cell culture medium separation device, thereby improving the applicability of the cell culture medium separation equipment.

[0007] To achieve the above objectives, the present invention provides the following technical solution: a cell culture medium separation device, comprising a support frame, a support plate on the upper part of the support frame, a plurality of test tube holders on the upper part of the support plate, a first drive motor fixedly installed on the lower part of the support plate, the first drive motor being used to drive the plurality of test tube holders to rotate synchronously, a fixed frame fixedly installed on the opposite side of the support frame above the support frame, a movable plate being provided inside the fixed frame, a plurality of rotating pressure plates adapted to the test tube holders being rotatably connected to the lower part of the movable plate, and a second drive motor fixedly installed on the upper part of the fixed frame for driving the movable plate to move up and down.

[0008] Preferably, a circular toothed ring is rotatably connected inside the bearing disk, and several gears that mesh with the circular toothed ring are rotatably connected inside the bearing disk, with the test tube seat located above the circular toothed ring.

[0009] Preferably, a mounting base is fixedly installed on the upper part of the gear, and a plug-in base is fixedly installed on the lower part of the test tube base. The upper part of the mounting base is provided with a plug-in groove that is adapted to the plug-in base, and a mounting bolt with one end threadedly connected to the side of the plug-in base passes through the side of the mounting base.

[0010] Preferably, the test tube holder is provided with several elastic abutment plates, and several movable rods penetrating the test tube holder are provided on one side of the abutment plates, with springs sleeved on the side of the movable rods.

[0011] Preferably, two symmetrical sliding blocks are fixedly installed on the side of the movable plate. Sliding grooves adapted to the sliding blocks are opened on the opposite side walls of the fixed frame. A drive screw with one end fixedly installed to the output end of the second drive motor is rotatably connected in the sliding groove. The drive screw passes through the sliding block and is threadedly connected to it. There are two second drive motors, which are synchronous motors.

[0012] Preferably, a fixing plate is fixedly installed between the ends of several movable rods, and a limiting seat is fixedly installed on the side of the abutment plate near the fixing plate. A limiting groove adapted to the limiting seat is opened on the side of the fixing plate near the abutment plate.

[0013] Preferably, the inner wall of the test tube holder has several clearance openings that are adapted to the fixing plate.

[0014] Preferably, the lower part of the abutment plate is provided with an arc-shaped elastic seat, which is arc-shaped and elastic, and the upper side of the abutment plate is provided with a guide bevel.

[0015] To address the shortcomings of existing technologies, this invention provides a cell culture medium separation device, overcoming these deficiencies. The beneficial effects of this invention are as follows:

[0016] In this invention, a first drive motor can drive several test tube holders to rotate simultaneously, enabling centrifugal separation of single or multiple cell culture medium test tubes, thereby improving the applicability of the cell culture medium separation equipment.

[0017] In this invention, the test tube holder is inserted into the insertion slot via a connector and fixed by mounting bolts. This allows the test tube holder to be replaced for test tubes of suitable size, further improving the applicability of the cell culture medium separation equipment.

[0018] In this invention, several abutment plates and springs are used, and the abutment plates are inserted into the limiting groove through the limiting seat, which can clamp and fix test tubes of different sizes, further improving the applicability of the cell culture medium separation device.

[0019] Other features and advantages of this invention will be set forth in the description which follows, and will be apparent in part from the description, or may be learned by practicing the invention. The objects and other advantages of this invention can be realized and obtained by means of the structures pointed out in the description, claims, and drawings. Attached Figure Description

[0020] The accompanying drawings are provided to further understand the present invention and form part of the specification. They are used together with the embodiments of the present invention to explain the present invention and do not constitute a limitation thereof.

[0021] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0022] Figure 2 This is a schematic cross-sectional view of the present invention.

[0023] Figure 3 This is a schematic diagram of the structure of several test tube holders in this utility model;

[0024] Figure 4 This is a schematic diagram of the internal structure of the bearing plate in this utility model;

[0025] Figure 5 This is a cross-sectional structural diagram of the test tube holder in this utility model;

[0026] Figure 6 for Figure 2 Enlarged structural diagram at point A in the middle;

[0027] Figure 7 for Figure 5 Enlarged structural diagram at point B;

[0028] Figure 8 for Figure 5 Enlarged structural diagram at point C;

[0029] Figure 9 for Figure 5 Enlarged structural diagram at point D.

[0030] In the diagram: 11. Support frame; 2. Support plate; 3. Test tube holder; 4. First drive motor; 5. Fixed frame; 6. Moving plate; 7. Rotating pressure plate; 8. Second drive motor; 9. Circular gear ring; 10. Gear; 11. Mounting seat; 12. Insertion seat; 13. Insertion groove; 14. Mounting bolt; 15. Abutment plate; 16. Moving rod; 17. Spring; 18. Sliding block; 19. Sliding groove; 20. Drive screw; 21. Fixed plate; 22. Limiting seat; 23. Limiting groove; 24. Clearance opening; 25. Arc-shaped elastic seat; 26. Guide angle. Detailed Implementation

[0031] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0032] Example 1

[0033] Please see Figures 1-9 A cell culture medium separation device includes a support frame 1, a support plate 2 on the upper part of the support frame 1, a plurality of test tube holders 3 on the upper part of the support plate 2, a first drive motor 4 fixedly installed on the lower part of the support plate 2, the first drive motor 4 being used to drive the plurality of test tube holders 3 to rotate synchronously, a fixed frame 5 fixedly installed on the opposite side of the support frame 1 above the support frame 1, a movable plate 6 provided inside the fixed frame 5, a plurality of rotating pressure plates 7 adapted to the test tube holders 3 being rotatably connected to the lower part of the movable plate 6, and a second drive motor 8 fixedly installed on the upper part of the fixed frame 5 for driving the movable plate 6 to move up and down. A circular toothed ring 9 is rotatably connected inside the bearing plate 2. Several gears 10 that mesh with the circular toothed ring 9 are rotatably connected inside the bearing plate 2. The test tube seat 3 is located above the circular toothed ring 9. Two symmetrical sliding blocks 18 are fixedly installed on the side of the moving plate 6. Sliding grooves 19 that are adapted to the sliding blocks 18 are opened on the opposite side walls inside the fixed frame 5. A drive screw 20 with one end fixedly installed to the output end of the second drive motor 8 is rotatably connected inside the sliding groove 19. The drive screw 20 passes through the sliding block 18 and is threadedly connected to it. There are two second drive motors 8, and they are synchronous motors.

[0034] The specific implementation method in this embodiment is as follows: When it is necessary to centrifuge the cell culture medium, the cell culture medium is quantitatively poured into test tubes, and the lower ends of several test tubes are inserted into the test tube holder 3. Then, two synchronous second drive motors 8 are turned on. The output end of the second drive motor 8 drives the drive screw 20 to rotate. When the drive screw 20 rotates, it drives the sliding block 18 to slide in the sliding groove 19. The two sliding blocks 18 drive the moving plate 6 to move down. The lower part of the rotating pressure plate 7 is designed to have an elastic effect. The moving plate 6 drives several rotating pressure plates 7 to move down. The rotating pressure plates 7 are tightly pressed against the upper end of the test tubes to seal the upper end of the test tubes. Then, the first drive motor 4 is turned on. The output end of the first drive motor 4 drives one of the gears 10 to rotate. When the gear 10 rotates, it drives the circular gear ring 9 to rotate. When the circular gear ring 9 rotates, it drives several other gears 10 to rotate. When the gears 10 rotate, they drive the test tube holder 3 to rotate, thereby achieving the effect of centrifuging the test tube holder 3. With the above structural settings, single or multiple cell culture medium test tubes can be centrifuged, improving the applicability of the cell culture medium separation equipment.

[0035] Example 2

[0036] Please see Figure 4 and Figure 9 This embodiment includes the above embodiment, and further includes: a mounting base 11 is fixedly installed on the upper part of the gear 10, a plug-in base 12 is fixedly installed on the lower part of the test tube base 3, the mounting base 11 has a plug-in groove 13 adapted to the plug-in base 12 on the upper part, and a mounting bolt 14 is threaded through the side of the mounting base 11, one end of which is threaded to the side of the plug-in base 12.

[0037] In this embodiment, the specific implementation method is as follows: When installing the test tube holder 3, the insertion seat 12 at the bottom of the test tube holder 3 is inserted into the insertion groove 13, and one end of the mounting bolt 14 passes through the mounting seat 11. The other end of the mounting bolt 14 is threadedly connected to one side of the insertion seat 12 to install and fix the test tube holder 3. This facilitates the installation or later replacement of the test tube holder 3 and allows for the replacement of the test tube holder 3 with test tubes of suitable size, further improving the applicability of the cell culture medium separation equipment.

[0038] Example 3

[0039] Please see Figure 5 , Figure 7 and Figure 8This embodiment includes all the above embodiments, and further includes: a plurality of elastic abutment plates 15 are provided inside the test tube holder 3; a plurality of moving rods 16 penetrating the test tube holder 3 are provided on one side of the abutment plate 15; springs 17 are sleeved on the side of the moving rods 16; a fixing plate 21 is fixedly installed between the ends of the plurality of moving rods 16; a limiting seat 22 is fixedly installed on the side of the abutment plate 15 near the fixing plate 21; a limiting groove 23 adapted to the limiting seat 22 is opened on the side of the fixing plate 21 near the abutment plate 15; a plurality of clearance openings 24 are opened on the inner side wall of the test tube holder 3; the clearance openings 24 are adapted to the fixing plate 21; an arc-shaped elastic seat 25 is provided at the lower part of the abutment plate 15; the arc-shaped elastic seat 25 is arc-shaped and elastic; and a guide angle 26 is opened on the upper side of the abutment plate 15.

[0040] In this embodiment, the test tube is installed by aligning its lower part with several abutment plates 15 and inserting it downwards through the guide angle 26. The abutment plates 15 push the moving rod 16 to move, at which point the spring 17 is compressed. The tension of the several springs 17 pushes the abutment plates 15 to clamp and fix the test tube. The arc-shaped elastic seat 25 protects the lower part of the test tube. The abutment plates 15 are inserted into the limiting groove 23 on one side of the fixing plate 21 through the limiting seat 22. The abutment plates 15 can be replaced, and different types of test tubes can be clamped and fixed, thus improving the applicability of the cell culture medium separation device. The opening 24 is used to accommodate the springs 17, increasing the range of test tubes that can be clamped.

[0041] All of the electrical products mentioned above can be purchased from the market. They are mature technologies and have been fully disclosed, so they will not be repeated in the instruction manual. All of the electrical products mentioned above are equipped with power cords, and they are electrically connected to the external main controller and 220V phase voltage (or 380V line voltage) through the power cords. The main controller can be a conventional known device such as a computer that plays a control role.

[0042] Finally, it should be noted that in the description of this utility model, the terms "vertical," "upper," "lower," "horizontal," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model.

[0043] In the description of this utility model, it should also be noted that, unless otherwise explicitly specified and limited, the terms "set," "install," "connect," and "link" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances.

[0044] The above description is merely a preferred embodiment of the present utility model and is not intended to limit the present utility model. Although the present utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. A cell culture fluid separation device comprising a carrier frame (1), characterized in that, The upper part of the support frame (1) is provided with a support plate (2), and the upper part of the support plate (2) is provided with several test tube seats (3). The lower part of the support plate (2) is fixedly installed with a first drive motor (4). The first drive motor (4) is used to drive several test tube seats (3) to rotate synchronously. The support frame (1) is fixedly installed on the opposite side with a fixed frame (5) located above the support frame (1). The fixed frame (5) is provided with a movable plate (6). The lower part of the movable plate (6) is rotatably connected with several rotating pressure plates (7) adapted to the test tube seats (3). The upper part of the fixed frame (5) is fixedly installed with a second drive motor (8) for driving the movable plate (6) to move up and down.

2. The cell culture fluid separation device of claim 1, wherein, A circular toothed ring (9) is rotatably connected inside the bearing plate (2), and several gears (10) that mesh with the circular toothed ring (9) are rotatably connected inside the bearing plate (2). The test tube seat (3) is located above the circular toothed ring (9).

3. The cell culture fluid separation device of claim 2, wherein, A mounting base (11) is fixedly installed on the upper part of the gear (10), and a plug-in base (12) is fixedly installed on the lower part of the test tube base (3). A plug-in groove (13) adapted to the plug-in base (12) is opened on the upper part of the mounting base (11), and a mounting bolt (14) with one end threaded to the side of the mounting base (11) is passed through it.

4. The cell culture fluid separation device of claim 1, wherein, The test tube holder (3) is provided with several elastic abutment plates (15), and several moving rods (16) that penetrate the test tube holder (3) are provided on one side of the abutment plates (15), and springs (17) are sleeved on the side of the moving rods (16).

5. The cell culture fluid separation device of claim 4, wherein, Two symmetrical sliding blocks (18) are fixedly installed on the side of the movable plate (6). The inner sidewalls of the fixed frame (5) are provided with sliding grooves (19) that are adapted to the sliding blocks (18). A drive screw (20) with one end fixedly installed to the output end of the second drive motor (8) is rotatably connected in the sliding groove (19). The drive screw (20) passes through the sliding block (18) and is threadedly connected to it. There are two second drive motors (8), and they are synchronous motors.

6. The cell culture fluid separation device of claim 4, wherein, A fixed plate (21) is fixedly installed between the ends of several movable rods (16). A limit seat (22) is fixedly installed on the side of the abutment plate (15) near the fixed plate (21). A limit groove (23) adapted to the limit seat (22) is opened on the side of the fixed plate (21) near the abutment plate (15).

7. The cell culture fluid separation device of claim 6, wherein, The inner wall of the test tube holder (3) has several clearance openings (24), which are adapted to the fixing plate (21).

8. The cell culture fluid separation device of claim 4, wherein, The lower part of the abutment plate (15) is provided with an arc-shaped elastic seat (25), which is arc-shaped and elastic. The upper side of the abutment plate (15) is provided with a guide angle (26).

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