A gene marker-assisted screening machine for the low-temperature domestication of white mushrooms from grasslands

Abstract

This utility model belongs to the field of edible fungi cultivation, specifically disclosing a low-temperature domestication gene marker-assisted screening machine for *Pleurotus ostreatus*, including a low-temperature incubator, a sealing cover, and a support frame. The support frame is movably installed on the top of the support frame. A movable plate is movably installed inside the low-temperature incubator at the bottom of the metal plate. Pull blocks are fixedly connected to both sides of the movable plate, and a magnetic block is fixedly connected to the top of the movable plate. A sealing cover is movably installed at the bottom of the movable plate, and a side plate is fixedly connected to the bottom of the sealing cover. An ultraviolet disinfection lamp is fixedly connected inside the low-temperature incubator at one end of the support frame. Through the cooperation of the movable plate, sealing cover, side plate, and limiting groove structure, this utility model can quickly seal the support frame after low-temperature treatment, preventing the *Pleurotus ostreatus* from being affected by sudden temperature changes and facilitating the transfer of the mushrooms by staff. The sealing cover also prevents the *Pleurotus ostreatus* from contacting external bacteria when removed, ensuring its cleanliness and quality.

Description

Technical Field

[0001] This utility model belongs to the field of edible fungi cultivation, specifically relating to a low-temperature domestication gene marker-assisted screening machine for grassland white mushrooms. Background Technology

[0002] As a fungus with high economic and nutritional value, the low-temperature domestication of *Pleurotus ostreatus* is of great significance for improving its environmental adaptability and expanding its cultivation range. Gene marker-assisted selection technology can precisely screen *Pleurotus ostreatus* strains with excellent low-temperature adaptability genes. The *Pleurotus ostreatus* low-temperature domestication gene marker-assisted screening machine is the key equipment for this process. It provides a stable low-temperature environment for *Pleurotus ostreatus*, and in conjunction with gene marker detection, efficiently completes the low-temperature domestication screening work, helping to select higher-quality *Pleurotus ostreatus* varieties.

[0003] Currently, the existing low-temperature domestication gene marker-assisted screening machines for *Pleurotus ostreatus* lack an independent sealing structure. After the low-temperature treatment, it is difficult to ensure that the *Pleurotus ostreatus* is in a sealed state when it is taken out, resulting in rapid temperature changes in the *Pleurotus ostreatus* and affecting the accuracy of low-temperature domestication screening. At the same time, external bacteria can easily come into contact with the *Pleurotus ostreatus*, increasing the risk of contamination and thus affecting the quality of the *Pleurotus ostreatus* and the reliability of the screening results. Utility Model Content

[0004] The purpose of this invention is to provide a low-temperature domestication gene marker-assisted screening machine for grassland white mushrooms to solve the problems mentioned in the background art.

[0005] To achieve the above objectives, this utility model provides the following technical solution:

[0006] A low-temperature domestication gene marker-assisted screening machine for *Pleurotus ostreatus* includes a low-temperature incubator, a sealing cover, and a support frame. A control panel is located on the top of the low-temperature incubator. A support frame is fixedly connected to the inside of the low-temperature incubator. A support frame is movably mounted on the top of the support frame. A metal plate is fixedly connected to the inside of the low-temperature incubator at the top of the support frame. A movable plate is movably mounted inside the low-temperature incubator at the bottom of the metal plate. Pull blocks are fixedly connected to both sides of the movable plate. A magnetic block is fixedly connected to the top of the movable plate. A sealing cover is movably mounted at the bottom of the movable plate. A side plate is fixedly connected to the bottom of the sealing cover. An ultraviolet disinfection lamp is fixedly connected to the inside of the low-temperature incubator at one end of the support frame.

[0007] Preferably, a sealing plate is fixedly connected to the periphery of the pull block, and the sealing plate is fitted into the low-temperature incubator.

[0008] Preferably, the support frame has limit grooves at both ends, and the side plate has a sealing block at the bottom, which fits into the limit groove.

[0009] Preferably, limit blocks are fixedly connected to both sides of the bottom of the movable plate, and the limit blocks are fitted into the sealing cover.

[0010] Preferably, a protective door is movably installed on the surface of the low-temperature incubator, and an observation window is embedded inside the protective door.

[0011] Preferably, the low-temperature incubator has movable grooves on both sides inside, and the pull block is fitted into the movable groove.

[0012] Compared with the prior art, the beneficial effects of this utility model are:

[0013] This invention places *Pleurotus ostreatus* in a support frame and places it in a low-temperature incubator. Utilizing the low-temperature environment and gene markers, precise low-temperature acclimatization and screening are achieved. Through the combination of a movable plate, sealing cap, side plates, and limiting groove structure, the support frame can be quickly sealed after low-temperature treatment, preventing the *Pleurotus ostreatus* from being affected by sudden temperature changes during removal. This allows staff more time to transfer the selected *Pleurotus ostreatus*. The sealing cap prevents the *Pleurotus ostreatus* from coming into contact with external bacteria during removal, ensuring its cleanliness and quality. Attached Figure Description

[0014] To more clearly illustrate the technical solutions of the embodiments of this utility model, the drawings used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort. Wherein:

[0015] Figure 1 This is a partial structural diagram of the sealing cap and support frame of this utility model;

[0016] Figure 2 This is a schematic diagram of the overall structure of this utility model;

[0017] Figure 3 This is a partial structural diagram of the movable plate and magnetic block of this utility model;

[0018] Figure 4 This is a partial structural diagram of the sealing cap and side plate of this utility model.

[0019] In the diagram: 1. Low-temperature incubator; 101. Control panel; 102. Movable slot; 103. Observation window; 104. Protective door; 2. Movable plate; 201. Pull block; 202. Limiting block; 203. Sealing plate; 204. Magnetic block; 3. Sealing cover; 4. Side plate; 401. Sealing block; 5. Support frame; 501. Limiting slot; 6. Support frame; 7. Metal plate; 8. Ultraviolet disinfection lamp. Detailed Implementation

[0020] To make the above-mentioned objectives, features and advantages of this utility model more apparent and understandable, the specific embodiments of this utility model will be described in detail below with reference to the accompanying drawings.

[0021] Many specific details are set forth in the following description in order to provide a full understanding of the present invention. However, the present invention may also be implemented in other ways different from those described herein. Those skilled in the art can make similar extensions without departing from the spirit of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0022] Secondly, the term "an embodiment" or "embodiment" as used herein refers to a specific feature, structure, or characteristic that may be included in at least one implementation of the present invention. The phrase "in one embodiment" appearing in different places in this specification does not necessarily refer to the same embodiment, nor is it a single or selective embodiment that excludes other embodiments.

[0023] As attached Figure 1 To be continued Figure 4 As shown:

[0024] Example 1: This example provides a low-temperature domestication gene marker-assisted screening machine for *Pleurotus ostreatus*, including a low-temperature incubator 1, a sealing cover 3, and a support frame 5. A control panel 101 is installed on the top of the surface of the low-temperature incubator 1. A support frame 6 is fixedly connected to the inside of the low-temperature incubator 1. The support frame 5 is movably installed on the top of the support frame 6. A metal plate 7 is fixedly connected to the inside of the low-temperature incubator 1 at the top of the support frame 5. A movable plate 2 is movably installed inside the low-temperature incubator 1 at the bottom of the metal plate 7. Pull blocks 2 are fixedly connected to both sides of the movable plate 2. 01. A magnetic block 204 is fixedly connected to the top of the movable plate 2. A sealing cover 3 is movably installed at the bottom of the movable plate 2. A side plate 4 is fixedly connected to the bottom of the sealing cover 3. An ultraviolet disinfection lamp 8 is fixedly connected inside the low-temperature incubator 1 at one end of the support frame 5. Limiting grooves 501 are provided at both ends inside the support frame 5. A sealing block 401 is provided at the bottom of the side plate 4, and the sealing block 401 is fitted with the limiting groove 501. Limiting blocks 202 are fixedly connected to both sides of the bottom of the movable plate 2, and the limiting blocks 202 are fitted with the sealing cover 3.

[0025] First, the staff placed the grassland white mushrooms to be screened on top of the support frame 5, then put the support frame 5 into the low temperature incubator 1 and placed it stably on the support frame 6. By operating the control panel 101, the low temperature incubator 1 started the cooling function to create an environment suitable for the low temperature acclimatization of grassland white mushrooms. Under the action of the low temperature environment, combined with relevant gene marker detection methods, the grassland white mushrooms were screened for low temperature acclimatization in order to determine their adaptability and gene characteristics under low temperature conditions.

[0026] After the low-temperature treatment is completed, the staff can pull down the pull block 201. The pull block 201 drives the movable plate 2 to descend synchronously. Since the bottom of the movable plate 2 is movably connected to the sealing cover 3, and the limiting blocks 202 on both sides of the movable plate 2 are engaged with the sealing cover 3, the movable plate 2 will drive the sealing cover 3 to descend together. During the descent, the sealing block 401 at the bottom of the side plate 4 gradually embeds into the limiting groove 501 of the support frame 5. At the same time, the sealing cover 3 fits against the support frame 5, achieving a seal on the support frame 5. When the staff opens the low-temperature incubator 1 and pulls out the support frame 5, the sealing cover 3 has formed an effective seal on the support frame 5, which can prevent the grassland white mushroom from changing temperature too quickly due to the high external temperature during the removal process, and also prevent contact with external bacteria. In addition, the ultraviolet disinfection lamp 8 inside the low-temperature incubator 1 can disinfect the internal environment before and after the equipment is used, ensuring the cleanliness of the screening environment; the metal plate 7 and the magnetic block 204 on the top of the movable plate 2 can use magnetic force to assist the stability or positioning of the movable plate 2 during the operation of the equipment, further improving the reliability of the equipment operation.

[0027] Example 2: This example is basically the same as the previous example, except that a sealing plate 203 is fixedly connected to the periphery of the pull block 201, and the sealing plate 203 is fitted into the low temperature incubator 1.

[0028] The sealing plate 203 can move with the pull block 201, and can seal the low temperature incubator 1 without affecting the movement of the pull block 201.

[0029] Specifically, a protective door 104 is movably installed on the surface of the low-temperature incubator 1, and an observation window 103 is embedded inside the protective door 104.

[0030] The protective door 104 can seal the low-temperature incubator 1, and the observation window 103 is made of tempered glass, allowing staff to observe the condition of the white prairie mushrooms inside the low-temperature incubator 1 through the observation window 103.

[0031] Specifically, both sides of the interior of the low-temperature incubator 1 are provided with movable grooves 102, and the pull block 201 is fitted into the movable grooves 102.

[0032] The pull block 201 can move inside the movable slot 102.

[0033] It is important to note that the constructions and arrangements of this application shown in several different exemplary embodiments are merely illustrative. Although only a few embodiments are described in detail in this disclosure, those who consult this disclosure will readily understand that many modifications are possible (e.g., changes in the size, dimensions, structure, shape and proportion of various elements, as well as parameter values ​​(e.g., temperature, pressure, etc.), mounting arrangements, use of materials, color, orientation, etc.) without substantially departing from the novel teachings and advantages of the subject matter described in this application). For example, an element shown as integrally formed may be composed of multiple parts or elements, the position of elements may be inverted or otherwise altered, and the nature or number or position of discrete elements may be changed or altered. Therefore, all such modifications are intended to be included within the scope of this utility model. The order or sequence of any process or method steps may be changed or reordered according to alternative embodiments. In the claims, any "device plus function" clause is intended to cover the structure described herein that performs the function, and not only structural equivalents but also equivalent structures. Without departing from the scope of this invention, other substitutions, modifications, alterations, and omissions may be made in the design, operation, and arrangement of the exemplary embodiments. Therefore, this invention is not limited to the specific embodiments, but extends to various modifications that still fall within the scope of the appended claims.

[0034] Furthermore, in order to provide a concise description of exemplary embodiments, not all features of actual embodiments (i.e., those features that are not relevant to the best mode of carrying out the present invention as currently considered, or those features that are not relevant to implementing the present invention) may be omitted.

[0035] It should be understood that numerous specific implementation decisions can be made during the development of any practical implementation, such as in any engineering or design project. Such development efforts may be complex and time-consuming, but for those skilled in the art who benefit from this disclosure, the development effort will be a routine work of design, manufacturing, and production without requiring much experimentation.

[0036] It should be noted that the above embodiments are only used to illustrate the technical solution of this utility model and are not intended to limit it. Although this utility model has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solution of this utility model without departing from the spirit and scope of the technical solution of this utility model, and all such modifications or substitutions should be covered within the scope of the claims of this utility model.

Claims

1. A low-temperature domestication genetic marker-assisted screening machine for grassland white mushroom, characterized by: It includes a low-temperature incubator (1), a sealing cover (3), and a support frame (5); ​ A control panel (101) is provided on the top of the surface of the low temperature incubator (1). A support frame (6) is fixedly connected to the inside of the low temperature incubator (1). A support frame (5) is movably installed on the top of the support frame (6). A metal plate (7) is fixedly connected to the inside of the low temperature incubator (1) on the top of the support frame (5). A movable plate (2) is movably installed inside the low temperature incubator (1) at the bottom of the metal plate (7). Pull blocks (201) are fixedly connected to both sides of the movable plate (2). A magnetic block (204) is fixedly connected to the top of the movable plate (2). A sealing cover (3) is movably installed at the bottom of the movable plate (2). A side plate (4) is fixedly connected to the bottom of the sealing cover (3). An ultraviolet disinfection lamp (8) is fixedly connected inside the low temperature incubator (1) at one end of the support frame (5).

2. The low temperature domestication genetic marker assisted screening machine for grassland white mushroom according to claim 1, characterized in that: The outer periphery of the pull block (201) is fixedly connected to a sealing plate (203), and the sealing plate (203) is fitted into the low temperature incubator (1).

3. The low-temperature domestication genetic marker-assisted screening machine for grassland white mushroom according to claim 1, characterized in that: The support frame (5) has a limiting groove (501) at both ends, and the side plate (4) has a sealing block (401) at the bottom, and the sealing block (401) is fitted with the limiting groove (501).

4. The low-temperature domestication genetic marker-assisted screening machine for leucopaxillus giganteus according to claim 1, characterized in that: Both sides of the bottom of the movable plate (2) are fixedly connected to limit blocks (202), and the limit blocks (202) are fitted with the sealing cover (3).

5. The low-temperature domestication genetic marker-assisted screening machine for Leucopaxillus giganteus according to claim 1, characterized in that: The surface of the low-temperature incubator (1) is movably fitted with a protective door (104), and an observation window (103) is embedded inside the protective door (104).

6. The low-temperature domestication gene marker-assisted screening machine for *Pleurotus ostreatus* according to claim 1, characterized in that: The low-temperature incubator (1) has movable slots (102) on both sides inside, and the pull block (201) is fitted into the movable slots (102).

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