Centrifugal Detection Device Based on Centrifugal Microfluidic Chip

Abstract

This utility model discloses a centrifugal detection device based on a centrifugal microfluidic chip, relating to the field of laboratory equipment technology. The device includes: a frame; a rotating mechanism mounted on the frame; a rotating body fixing seat connected to the rotating mechanism; a chip loading mechanism for lifting and separating or lowering and engaging the centrifugal microfluidic chip relative to the rotating body fixing seat; the rotating body fixing seat is driven by the rotating mechanism to rotate the centrifugal microfluidic chip attached thereto; a heating mechanism disposed within the chip loading mechanism for heating the centrifugal microfluidic chip mounted on the chip loading mechanism; and a result detection component mounted on the frame for detecting the reaction results of the centrifugal microfluidic chip. The centrifugal detection device provided by this utility model is compact and easy to automate.

Description

Technical Field

[0001] This utility model relates to a laboratory device, specifically a centrifugal detection device based on a centrifugal microfluidic chip. Background Technology

[0002] Centrifugal microfluidic chip systems refer to microfluidic systems that utilize microelectromechanical processing (MEMS) technology to miniaturize and integrate components such as valves, flow channels, mixing reactors, heaters, separation devices, and detectors involved in chemical analysis processes including sampling, pretreatment, derivatization, mixing, and detection onto a CD-shaped chip. Using centrifugal force as the driving force for fluid flow, they achieve microfluidic system detection and analysis of fluid flow. When this microfluidic system is combined with temperature control, detection, and the emerging information storage and processing technologies, it forms a system capable of performing various comprehensive analytical functions. As the application of centrifugal microfluidic chips becomes more widespread, the demand for supporting centrifuge equipment is also constantly increasing. However, currently available centrifuge equipment has relatively limited functionality, is bulky, and is not conducive to automated integration.

[0003] The information disclosed in this background section is intended only to enhance the understanding of the overall background of this utility model and should not be construed as an admission or in any way implying that the information constitutes prior art known to those skilled in the art. Utility Model Content

[0004] Purpose of the utility model: The technical problem to be solved by this utility model is to provide a centrifugal detection device based on a centrifugal microfluidic chip, which can support functions such as high-speed centrifugation, positioning detection, heating and incubation, and automatic disk loading, and is small in size and easy to be automated.

[0005] To address the aforementioned technical problems, this utility model discloses a centrifugal detection device based on a centrifugal microfluidic chip. The device includes:

[0006] frame;

[0007] A rotating mechanism, which is mounted on the frame;

[0008] A rotating body fixing seat, wherein the rotating body fixing seat is connected to the rotating mechanism in a transmission manner;

[0009] A chip loading mechanism is used to lift and separate the centrifugal microfluidic chip relative to the rotating body fixture or lower and engage it; the rotating body fixture can be driven by the rotating mechanism to rotate the centrifugal microfluidic chip engaged thereon.

[0010] A heating mechanism is disposed within the chip loading mechanism and is used to heat the centrifugal microfluidic chip carried on the chip loading mechanism;

[0011] And a result detection component, which is mounted on the rack, for detecting the reaction results of the centrifugal microfluidic chip.

[0012] Specifically, the rotating mechanism includes a high-speed rotating motor, a motor driver for controlling the rotational speed of the high-speed rotating motor shaft, and a positioning code disk fixed to the bottom of the high-speed rotating motor. The rotating body fixing seat is connected to the rotational shaft of the high-speed rotating motor, and the high-speed rotating motor and the positioning code disk are respectively electrically connected to the motor driver.

[0013] More specifically, the high-speed rotary motor is a DC brushless rotary motor.

[0014] Specifically, the chip loading mechanism includes a lifting disk tray and a Z-axis linear motion mechanism. The Z-axis linear motion mechanism is mounted on the frame and connected to the lifting disk tray. The lifting disk tray, driven by the Z-axis linear motion mechanism, can lift and separate or lower and engage the centrifugal microfluidic chip carried on the lifting disk tray relative to the rotating body fixed seat.

[0015] More specifically, the Z-axis linear motion mechanism includes a lead screw rotatably mounted on the frame, a slider seat threadedly connected to the lead screw, a connecting rod, and a reset photoelectric sensor. The upper end of the connecting rod is fixedly connected to the lifting disc tray, and the lower end of the connecting rod is fixedly connected to the slider seat. The reset photoelectric sensor is mounted on the frame and is used to sense whether the lifting disc tray has risen to the starting position.

[0016] Furthermore, the device also includes a disc cap connected to the upper side of the lifting disc tray; the lifting disc tray moves between an upper unloading position and a lower loading position to drive the centrifugal microfluidic chip carried on the lifting disc tray to rise and separate or fall and engage relative to the rotating body fixed seat; when the lifting disc tray is in the lower loading position, the disc cap is located above the engaged centrifugal microfluidic chip and is in clearance fit with the centrifugal microfluidic chip.

[0017] Specifically, the gap ranges from 1 to 2 millimeters.

[0018] Furthermore, the device also includes a guide sleeve, which is mounted on the frame, and the connecting rod is slidably sleeved within the guide sleeve.

[0019] Specifically, the heating mechanism is installed inside the lifting disc tray.

[0020] Optionally, the result detection component is selected from one of the imaging detection component, the absorbance detection component, and the fluorescence detection component.

[0021] Beneficial effects:

[0022] 1. The centrifugal detection device based on centrifugal microfluidic chip provided by this utility model can support functions such as high-speed centrifugation, positioning detection, heating incubation and automatic disk loading, and is small in size and easy to be automated.

[0023] 2. This invention utilizes a lifting disc tray to raise and separate the centrifugal microfluidic chip relative to the rotating fixed base, or lower and engage it. Simultaneously, by placing the heating mechanism inside the lifting disc tray, heat conduction is achieved or interrupted by controlling the tray's ascent to contact or descent to disengage from the microfluidic chip. Compared to existing air-conduction heating methods, this invention offers higher heating efficiency and temperature accuracy; it also overcomes the drawback of existing metal-conduction heating methods that cannot automatically interrupt heat conduction. Attached Figure Description

[0024] The present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments, and the advantages of the present invention in the above and / or other aspects will become clearer.

[0025] Figure 1 The schematic diagram of the centrifugal detection device based on the centrifugal microfluidic chip provided in this embodiment does not include the centrifugal microfluidic chip.

[0026] Figure 2 This is a schematic diagram of the centrifugal detection device based on a centrifugal microfluidic chip provided in this embodiment, which carries a centrifugal microfluidic chip.

[0027] Figure 3 This is a schematic diagram of the assembly structure of the rotating mechanism, the rotating body fixing seat and the frame provided in this embodiment.

[0028] Figure 4 This is a schematic diagram of the chip loading mechanism provided in this embodiment.

[0029] Figure 5 This is a top view of the connection structure between the rotating body fixture and the centrifugal microfluidic chip provided in this embodiment.

[0030] Figure 6 for Figure 5 A magnified view of the K region.

[0031] The accompanying labeling is as follows:

[0032] 10. Rotating mechanism; 20. Chip loading mechanism; 30. Result detection component; 40. Centrifugal microfluidic chip; 101. High-speed rotary motor; 102. Positioning encoder; 103. Motor driver; 104. Rotating body fixing seat; 105. Frame; 201. Lead screw; 202. Lifting disc tray; 203. Disc cover; 204. Reset photoelectric sensor; 205. Connecting rod; 206. Slider seat; Guide sleeve 207. Detailed Implementation

[0033] like Figures 1 to 6 The centrifugal detection device based on a centrifugal microfluidic chip provided in this embodiment includes: a frame 105; a rotating mechanism 10 mounted on the frame 105; a rotating body fixing seat 104 connected to the rotating mechanism 10; a chip loading mechanism 20 used to lift and separate the centrifugal microfluidic chip 40 relative to the rotating body fixing seat 104 or lower and engage it; the rotating body fixing seat 104 can be driven by the rotating mechanism 10 to rotate the centrifugal microfluidic chip 40 engaged thereon; a heating mechanism disposed in the chip loading mechanism 20 for heating the centrifugal microfluidic chip 40 carried on the chip loading mechanism 20; and a result detection component 30 mounted on the frame 105 for detecting the reaction result of the centrifugal microfluidic chip 40.

[0034] Specifically, such as Figure 3 As shown, the rotating mechanism 10 includes a high-speed rotating motor 101, a motor driver 103 for controlling the rotational speed of the shaft of the high-speed rotating motor 101, and a positioning code disk 102 fixed to the bottom of the high-speed rotating motor 101. The rotating body fixing seat 104 is connected to the shaft of the high-speed rotating motor 101 through a transmission connection. The high-speed rotating motor 101 and the positioning code disk 102 are electrically connected to the motor driver 103 respectively.

[0035] In this embodiment, the high-speed rotating motor 101 can achieve high-speed rotation under the control of the motor driver 103, and the positioning encoder 102 can cooperate with the motor driver 103 to complete angular displacement control, thereby controlling the high-speed rotating motor 101 to perform high-precision positioning motion.

[0036] Specifically, the high-speed rotary motor 101 is a DC brushless rotary motor.

[0037] Specifically, such as Figure 4As shown, the chip loading mechanism 20 includes a lifting disk tray 202 and a Z-axis linear motion mechanism. The Z-axis linear motion mechanism is mounted on the frame 105 and is connected to the lifting disk tray 202. The lifting disk tray 202 can drive the centrifugal microfluidic chip 40 carried on the lifting disk tray 202 to be lifted and separated or lowered and engaged relative to the rotating body fixed seat 104 under the drive of the Z-axis linear motion mechanism.

[0038] More specifically, such as Figure 4 As shown, the Z-axis linear motion mechanism includes a lead screw 201 rotatably mounted on the frame 105, a slider seat 206 threadedly connected to the lead screw 201, a connecting rod 205, a reset photoelectric sensor 204, and a drive motor for driving the lead screw 201 to rotate. The upper end of the connecting rod 205 is fixedly connected to the lifting disc tray 202, and the lower end of the connecting rod 205 is fixedly connected to the slider seat 206. The reset photoelectric sensor 204 is mounted on the frame 105 and is used to sense whether the lifting disc tray 202 has moved upward to the starting position. Whenever the lifting disc tray 202 moves to the starting position, the reset photoelectric sensor 204 senses whether the lifting disc tray 202 has reached the starting position. If the starting position is reached, the reset photoelectric sensor 204 will send a positioning signal, causing the Z-axis linear motion mechanism to control the lifting disc tray 202 to stop moving upward.

[0039] Furthermore, such as Figure 4 As shown, it also includes a disk cover 203, which is connected to the upper part of one side of the lifting disk tray 202. The disk cover 203 and the lifting disk tray 202 form a receiving cavity for receiving and accommodating the centrifugal microfluidic chip 40. The lifting disk tray 202 moves between the upper unloading position and the lower loading position to drive the centrifugal microfluidic chip 40 carried on the lifting disk tray 202 to be lifted and separated or lowered and engaged relative to the rotating body fixed seat 104. When the lifting disk tray 202 is in the lower loading position, the disk cover 203 is located above the engaged centrifugal microfluidic chip 40 and is clearance-fitted with the centrifugal microfluidic chip 40.

[0040] Preferably, the gap ranges from 1 to 2 millimeters.

[0041] Furthermore, such as Figure 4 As shown, it also includes a guide sleeve 207, which is installed on the frame 105, and the connecting rod 205 is slidably sleeved in the guide sleeve 207.

[0042] like Figure 5 and Figure 6As shown, the outer ring of the rotating body holder 104 is polygonal. The corresponding centrifugal microfluidic chip 40 has a slightly larger inner diameter polygonal inner hole, allowing it to easily fall onto the rotating body holder 104. When the inner hole of the centrifugal microfluidic chip 40 and the rotating body holder 104 are displaced relative to each other by a certain distance, they become tangent, at which point they achieve self-locking. The connection structure between the rotating body holder 104 and the centrifugal microfluidic chip 40 can adopt the connecting device for a rotating body used in medical testing provided in Chinese Utility Model Patent Publication No. CN222510960U.

[0043] Specifically, the heating mechanism is installed inside the lifting disc tray 202.

[0044] In this embodiment, the heating mechanism heats the centrifugal microfluidic chip 40 through the lifting disc tray 202, providing a warm environment for the centrifugal microfluidic chip 40.

[0045] Specifically, the result detection component 30 uses an industrial camera, which integrates an imaging light source. The centrifugal microfluidic chip 40 can rotate to any position under the drive of the rotating mechanism 10. The result detection component 30 takes pictures of each reaction site of the centrifugal microfluidic chip 40 and uploads the pictures to an external computer for user analysis. The result detection component 30 is not limited to imaging detection; it can be replaced to support absorbance detection, fluorescence detection, etc. The result detection component 30 can use existing result detection components, and its specific configuration is not a major improvement of this application, so it will not be described in detail here.

[0046] The working process of the centrifugal testing device provided by this utility model is described in detail below.

[0047] like Figure 2 As shown, the centrifugal microfluidic chip 40 is placed on the lifting disc tray 202. Driven by the slider seat 206 and guided by the guiding mechanism formed by the guide sleeve 207 and the connecting rod 205, the lifting disc tray 202 moves linearly downwards along the Z-axis. The centrifugal microfluidic chip 40 automatically mounts onto the rotating body fixing seat 104. At this point, the centrifugal microfluidic chip 40 is still in contact with the lifting disc tray 202. Subsequently, the lifting disc tray 202 continues to descend a certain height along the Z-axis until it completely detaches from the centrifugal microfluidic chip 40, thus completing the automatic loading of the centrifugal microfluidic chip 40.

[0048] After receiving the running command from the external software, the motor driver 103 controls the high-speed rotary motor 101 to start rotating. The rotating body fixed seat 104 is driven by the high-speed rotary motor 101 to rotate slowly in a clockwise direction for tens of seconds. At this time, due to inertia, in the initial stage of the rotation of the rotating body fixed seat 104, since the inner diameter of the central hole of the centrifugal microfluidic chip 40 is slightly larger than the outer diameter of the rotating body fixed seat 104, there will be a relative displacement in the circumferential direction between the centrifugal microfluidic chip 40 and the rotating body fixed seat 104. Until it moves to a certain distance, the central hole of the centrifugal microfluidic chip 40 is tangent to the rotating body fixed seat 104. At this time, the rotating body fixed seat 104 will self-lock with the central hole of the centrifugal microfluidic chip 40 to ensure that there will be no more relative movement between the two.

[0049] The centrifugal microfluidic chip 40 is driven by a high-speed rotating motor 101 to begin high-speed centrifugal motion in a clockwise direction. After a certain period of motion, the motor driver 103 controls the high-speed rotating motor 101 to stop rotating. The lifting disc tray 202, driven by the slider seat 206 and guided by the guiding mechanism formed by the guide sleeve 207 and the connecting rod 205, moves linearly upward along the Z-axis until it contacts the centrifugal microfluidic chip 40. The heating mechanism is then activated to heat the lifting disc tray 202, which provides a warm environment for the centrifugal microfluidic chip 40, accelerating the reaction rate of the reagents within the chip. After the reaction is complete, the lifting disc tray 202 descends to a certain height and detaches from the centrifugal microfluidic chip 40.

[0050] After the reaction is completed, the centrifugal microfluidic chip 40 is rotated to a designated position by the rotating mechanism 10. The result detection component 30 is used to detect the reaction result of each reaction hole on the centrifugal microfluidic chip 40. The result detection component 30 can be an imaging detection or a detection of various optical signals. The result detection component 30 can be replaced as needed.

[0051] In this application, the Z-axis direction is the vertical direction.

[0052] This invention provides a concept and method for a centrifugal detection device based on a centrifugal microfluidic chip. Many methods and approaches exist for implementing this technical solution; the above description is merely a preferred embodiment. It should be noted that those skilled in the art can make various improvements and modifications without departing from the principles of this invention, and these improvements and modifications should also be considered within the scope of protection of this invention. All components not explicitly stated in this embodiment can be implemented using existing technology.

Claims

1. A centrifugal detection device based on a centrifugal microfluidic chip, characterized in that, include: Rack (105); A rotating mechanism (10) is mounted on the frame (105); Rotating body fixing seat (104), the rotating body fixing seat (104) is connected to the rotating mechanism (10) in a transmission manner; The chip loading mechanism (20) is used to drive the centrifugal microfluidic chip (40) to be lifted and separated or lowered and engaged relative to the rotating body fixing seat (104); the rotating body fixing seat (104) can be driven by the rotating mechanism (10) to drive the centrifugal microfluidic chip (40) engaged thereon to rotate. A heating mechanism is provided inside the chip loading mechanism (20) for heating the centrifugal microfluidic chip (40) carried on the chip loading mechanism (20); And a result detection component (30), which is mounted on the rack (105) and is used to detect the reaction results of the centrifugal microfluidic chip (40).

2. The centrifugal detection device based on the centrifugal microfluidic chip according to claim 1, characterized in that, The rotating mechanism (10) includes a high-speed rotating motor (101), a motor driver (103) for controlling the rotational speed of the shaft of the high-speed rotating motor (101), and a positioning code disk (102) fixed to the bottom of the high-speed rotating motor (101). The rotating body fixing seat (104) is connected to the shaft of the high-speed rotating motor (101) through a transmission connection. The high-speed rotating motor (101) and the positioning code disk (102) are respectively electrically connected to the motor driver (103).

3. The centrifugal detection device based on the centrifugal microfluidic chip according to claim 2, characterized in that, The high-speed rotary motor (101) is a DC brushless rotary motor.

4. The centrifugal detection device based on the centrifugal microfluidic chip according to claim 1, characterized in that, The chip loading mechanism (20) includes a lifting disk tray (202) and a Z-axis linear motion mechanism. The Z-axis linear motion mechanism is mounted on the frame (105) and is connected to the lifting disk tray (202). The lifting disk tray (202) can drive the centrifugal microfluidic chip (40) carried on the lifting disk tray (202) to lift and separate or lower and engage relative to the rotating body fixing seat (104) under the drive of the Z-axis linear motion mechanism.

5. The centrifugal detection device based on the centrifugal microfluidic chip according to claim 4, characterized in that, The Z-axis linear motion mechanism includes a lead screw (201) rotatably mounted on the frame (105), a slider seat (206) threadedly connected to the lead screw (201), a connecting rod (205), and a reset photoelectric sensor (204). The upper end of the connecting rod (205) is fixedly connected to the lifting disc tray (202), and the lower end of the connecting rod (205) is fixedly connected to the slider seat (206). The reset photoelectric sensor (204) is mounted on the frame (105) and is used to sense whether the lifting disc tray (202) has risen to the starting position.

6. The centrifugal detection device based on the centrifugal microfluidic chip according to claim 4, characterized in that, It also includes a disc cap (203), which is connected to the upper part of one side of the lifting disc tray (202); the lifting disc tray (202) moves between the upper unloading position and the lower loading position to drive the centrifugal microfluidic chip (40) carried on the lifting disc tray (202) to be lifted and separated or lowered and engaged relative to the rotating body fixing seat (104); when the lifting disc tray (202) is in the lower loading position, the disc cap (203) is located above the engaged centrifugal microfluidic chip (40) and is clearance-fitted with the centrifugal microfluidic chip (40).

7. The centrifugal detection device based on the centrifugal microfluidic chip according to claim 6, characterized in that, The gap ranges from 1 to 2 millimeters.

8. The centrifugal detection device based on the centrifugal microfluidic chip according to claim 5, characterized in that, It also includes a guide sleeve (207), which is installed on the frame (105), and the connecting rod (205) is slidably sleeved in the guide sleeve (207).

9. The centrifugal detection device based on the centrifugal microfluidic chip according to claim 5, characterized in that, The heating mechanism is installed inside the lifting disc tray (202).

10. The centrifugal detection device based on the centrifugal microfluidic chip according to claim 1, characterized in that, The result detection component (30) is selected from one of the imaging detection component, absorbance detection component and fluorescence detection component.

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