processing device

Abstract

The application relates to a processing device. The processing device comprises a host, a switching valve and a collecting pipe. The host comprises a shell assembly and a piston. The shell assembly is provided with a buffer cavity and a plurality of accommodation cavities penetrating through the shell assembly along the axial direction of the host. The shell assembly is also provided with a collecting cavity. The plurality of accommodation cavities and the collecting cavity are arranged at intervals around the buffer cavity. The piston is slidingly arranged in the buffer cavity. The switching valve comprises a valve assembly rotatably connected with the shell assembly. The valve assembly is provided with a switching channel. The switching channel is provided with a first switching port and a second switching port arranged at intervals. When the valve assembly rotates, the first switching port is always in communication with the buffer cavity. The second switching port can be in communication with the collecting cavity and different accommodation cavities. The collecting cavity can be in communication with the pipe cavity of the collecting pipe. Thus, the applicability of the processing device to various working conditions can be improved.

Description

Technical Field

[0001] This application relates to the field of medical device technology, and in particular to a processing device. Background Technology

[0002] Fully automated nucleic acid extraction is one of the core supporting technologies in modern biomedicine. Through highly integrated and intelligent equipment, it achieves the efficient and precise separation and purification of nucleic acids (DNA / RNA) from biological samples. Fully automated nucleic acid extraction utilizes processing devices that integrate steps such as sample lysis, nucleic acid adsorption, washing, and elution using chemical and physical methods to achieve nucleic acid extraction. However, traditional processing devices often suffer from the limitation of single-function operation, thus affecting their applicability to various operating conditions. Utility Model Content

[0003] One technical problem addressed by this application is how to improve the applicability of the processing device to various operating conditions.

[0004] A processing apparatus, comprising:

[0005] The host includes a shell assembly and a piston. The shell assembly has a buffer cavity and a plurality of receiving cavities that extend through the shell assembly along the axial direction of the host. The shell assembly also has a collection cavity. The plurality of receiving cavities and the collection cavity are arranged at intervals around the buffer cavity. The piston is slidably disposed within the buffer cavity.

[0006] An exchange valve includes a valve assembly rotatably connected to the housing assembly. The valve assembly has an exchange channel with a first exchange port and a second exchange port spaced apart. When the valve assembly rotates, the first exchange port is always in communication with the buffer cavity, and the second exchange port is capable of communicating with the collection cavity and different accommodating cavities.

[0007] A collection tube is connected to the shell assembly, and the collection cavity is in communication with the lumen of the collection tube.

[0008] In one embodiment, the shell assembly is further provided with a ventilation hole, which connects the cavity of the collection tube to the outside.

[0009] In one embodiment, the shell assembly includes a shell and a cover. The shell includes a base plate, an inner cylinder, an outer cylinder, and a partition. The inner cylinder and the outer cylinder both protrude from the base plate. The outer cylinder surrounds the inner cylinder. The partition is connected to the inner cylinder and / or the inner cylinder. The space enclosed by the inner cylinder is configured as part of the buffer cavity. The space between the partitions is configured as part of the receiving cavity and the collecting cavity.

[0010] In one embodiment, the cover includes a first cover, a second cover, and a shielding membrane. The first cover is fixedly connected to the housing, and the second cover is movably connected to the first cover to open or close the first cover. The first cover and the second cover are provided with a buffer hole and a receiving hole. The buffer hole is configured as part of the buffer cavity, and the receiving hole is configured as part of the receiving cavity. A communicating groove is recessed on the surface of the first cover facing the second cover. The communicating groove communicates the collecting cavity and the lumen of the collecting tube. The shielding membrane is used to seal the opening of the communicating groove.

[0011] In one embodiment, the collection tube is detachably connected to the shell assembly.

[0012] In one embodiment, a reaction mechanism is also included. The shell assembly is further provided with a first flow channel. The reaction mechanism is detachably connected to the shell assembly and is provided with a reaction chamber and a first reaction channel. The reaction chamber can connect the first reaction channel to the outside. The first reaction channel is connected to the first flow channel. When the valve assembly rotates, the second exchange port can connect to the first flow channel.

[0013] In one embodiment, the exchange channel further has a pressurization port, which is spaced apart from the first exchange port and the second exchange port. When the valve assembly rotates, the pressurization port can communicate with the first flow channel.

[0014] In one embodiment, the shell assembly further has a second flow channel and a vent chamber spaced apart, the reaction mechanism further has a second reaction channel, the second reaction channel is connected to the reaction chamber and the second flow channel; the exchange valve further has a transfer channel independently arranged relative to the exchange channel, the transfer channel has a first transfer port and a second transfer port spaced apart, when the second exchange port is connected to the first flow channel, the first transfer port is connected to the vent chamber, and the second transfer port is connected to the second flow channel.

[0015] In one embodiment, the reaction mechanism includes a reaction plate and a cover film. The reaction plate is connected to the shell assembly. The reaction cavity extends through the reaction plate along its thickness direction. The number of cover films includes two. The two cover films are located on opposite sides of the reaction plate in the thickness direction and cover the reaction cavity. The first reaction channel is located inside the reaction plate.

[0016] In one embodiment, the host further includes a liquid-sealing and venting membrane disposed on the housing assembly and sealing the openings of the buffer chamber, the collection chamber, and the receiving chamber at the end away from the exchange valve.

[0017] One technical advantage of one embodiment of this application is that when the valve assembly rotates, the first exchange port is always connected to the buffer chamber, and the second exchange port can communicate with different accommodating chambers. This allows liquid to transfer between different accommodating chambers through the buffer chamber, thereby processing the liquid to extract nucleic acid products. The nucleic acid products can be stored in the collection tube, thus realizing the storage of nucleic acid products in the collection tube. This achieves the automatic storage and collection function of the processing device for nucleic acid products, effectively avoiding situations where the processing device cannot automatically store and collect nucleic acid products, thereby improving the versatility of the processing device's functions and ultimately enhancing its applicability to various operating conditions. Attached Figure Description

[0018] Figure 1 This is a three-dimensional structural schematic diagram of a processing device provided in one embodiment.

[0019] Figure 2 for Figure 1 A schematic diagram of the exploded structure of the processing device shown.

[0020] Figure 3 for Figure 1 A partial exploded view of the processing device shown.

[0021] Figure 4 for Figure 1 The diagram shows a three-dimensional structural schematic of the processing device when the second cover is in the open state.

[0022] Figure 5 for Figure 4 A schematic diagram of its decomposed structure.

[0023] Figure 6 for Figure 1 The schematic diagram shows the three-dimensional cross-sectional structure of the processing device in its disassembled state.

[0024] Figure 7 for Figure 1 The diagram shows a partial planar structure of the processing device after the exchange valve has been removed.

[0025] Figure 8 for Figure 1 An exploded view of the valve assembly in the processing device shown.

[0026] Figure 9 for Figure 8 A schematic diagram of the planar structure of the actuator in the valve assembly shown.

[0027] Figure 10 for Figure 8 A schematic diagram of the planar structure of the valve body in the valve assembly shown.

[0028] Figure 11 for Figure 1The diagram shows a partial three-dimensional cross-sectional view of the processing device after the second cover has been removed.

[0029] Reference numerals: Processing device 10, Main unit 100, Shell assembly 110, Buffer cavity 111, First buffer port 1111, Second buffer port 1112, Receiving cavity 112, First receiving port 1121, Second receiving port 1122, Vent cavity 113, Collection cavity 114, First guide channel 115, Second guide channel 116, Shell 117, Base plate 1171, Inner cylinder 1172, Outer cylinder 1173, Partition 1174, Plane 1175, Cover 118, First cover 1181, Connecting groove 1181a, Protrusion 1181b, Second cover 1182, Rotating part 1182a, Connecting part 1182b, Buffer hole 1183, Receiving hole 1184, Shielding membrane 1185, Ventilation hole 11 86. Seal 119, Piston 120, Liquid-barrier and breathable membrane 130, Exchange valve 200, Valve assembly 210, Exchange channel 211, First exchange port 2111, Second exchange port 2112, Pressurization port 2113, Exchange groove 2114, First exchange hole 2115, Second exchange hole 2116, Transfer channel 212, First transfer port 2121, Second transfer port 2122, Transfer groove 2123, First transfer hole 2124, Second transfer hole 2125, Drive 213, Valve body 214, Bearing 220, Reaction mechanism 300, Reaction chamber 330, First reaction channel 310, Second reaction channel 320, Reaction plate 340, Covering membrane 350, Connector 360, Collection pipe 400. Detailed Implementation

[0030] To make the above-mentioned objectives, features, and advantages of this application more apparent and understandable, the specific embodiments of this application are described in detail below with reference to the accompanying drawings. Many specific details are set forth in the following description to provide a thorough understanding of this application. However, this application can be implemented in many other ways different from those described herein, and those skilled in the art can make similar modifications without departing from the spirit of this application. Therefore, this application is not limited to the specific embodiments disclosed below.

[0031] In the description of this application, it should be understood that if terms such as "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential" appear, these terms indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this application.

[0032] Furthermore, where the terms "first" and "second" appear, these terms are for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Thus, a feature defined with "first" or "second" may explicitly or implicitly include at least one of that feature. In the description of this application, where the term "multiple" appears, "multiple" means at least two, such as two, three, etc., unless otherwise explicitly specified.

[0033] In this application, unless otherwise expressly specified and limited, the terms "installation," "connection," "joining," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components, unless otherwise expressly limited. Those skilled in the art can understand the specific meaning of the above terms in this application based on the specific circumstances.

[0034] In this application, unless otherwise expressly specified and limited, the use of descriptions such as "above" or "below" the second feature indicates that the first and second features are in direct contact or indirect contact via an intermediate medium. Furthermore, "above," "on top of," and "over" the second feature can mean that the first feature is directly above or diagonally above the second feature, or simply that the first feature is at a higher horizontal level than the second feature. Similarly, "below," "below," and "under" the second feature can mean that the first feature is directly below or diagonally below the second feature, or simply that the first feature is at a lower horizontal level than the second feature.

[0035] It should be noted that if an element is referred to as being "fixed to" or "set on" another element, it can be directly on the other element or there may be an intervening element. If an element is considered to be "connected to" another element, it can be directly connected to the other element or there may be an intervening element. If so, the terms "vertical," "horizontal," "upper," "lower," "left," "right," and similar expressions used in this application are for illustrative purposes only and do not represent the only possible implementation.

[0036] See Figure 1 , Figure 2 , Figure 3 and Figure 6This application provides a processing device 10 for processing nucleic acids. The processing device 10 includes a main unit 100 and an exchange valve 200. The main unit 100 includes a shell assembly 110, a piston 120, and a liquid-barrier and gas-permeable membrane 130. The shell assembly 110 has a buffer cavity 111 and a receiving cavity 112. There is one buffer cavity 111 and multiple receiving cavities 112. The buffer cavity 111 can be located at the center of the shell assembly 110. The multiple receiving cavities 112 are arranged at intervals around the buffer cavity 111. The buffer cavity 111 and the receiving cavity 112 penetrate the entire shell assembly 110 along the axial direction, so that both the buffer cavity 111 and the receiving cavity 112 have openings at the ends of the shell assembly 110 along the axial direction. For example, the buffer cavity 111 has a first buffer port 1111 and a second buffer port 1112 formed at its two ends, respectively. The second buffer port 1112 is further away from the exchange valve 200 relative to the first buffer port 1111, so that the second buffer port 1112 can be located above the first buffer port 1111. The receiving cavity 112 has a first receiving port 1121 and a second receiving port 1122 formed at its two ends, respectively. The second receiving port 1122 is further away from the exchange valve 200 relative to the first receiving port 1121, so that the second receiving port 1122 can be located above the first receiving port 1121. A liquid-proof and ventilated membrane 130 is disposed on the housing assembly 110. The liquid-proof and ventilated membrane 130 covers the second buffer port 1112 and the second receiving port 1122. The liquid-proof and ventilated membrane 130 prevents liquid from passing through but allows gas to pass through, thus preventing liquid in the buffer chamber 111 from leaking through the liquid-proof and ventilated membrane 130 from leaking through the second buffer port 1112, and also preventing liquid in the receiving chamber 112 from leaking through the liquid-proof and ventilated membrane 130 from leaking through the second receiving port 1122. A piston 120 is slidably disposed within the buffer chamber 111.

[0037] See Figure 2 , Figure 3 , Figure 7 and Figure 8 The exchange valve 200 includes a valve assembly 210, which is rotatably connected to the housing assembly 110. For example, the valve assembly 210 can rotate about the central axis of the housing assembly 110. The valve assembly 210 has an exchange channel 211, which has a first exchange port 2111 and a second exchange port 2112. The first exchange port 2111 and the second exchange port 2112 are spaced apart. When the valve assembly 210 rotates, the first exchange port 2111 is always connected to the first buffer port 1111, and the second exchange port 2112 is alternately connected to different first receiving ports 1121.

[0038] When piston 120 moves near exchange valve 200 in buffer chamber 111, i.e., when piston 120 moves downward, the pressure in buffer chamber 111 increases. This allows liquid in buffer chamber 111 to enter exchange channel 211 through first buffer port 1111 and first exchange port 2111, and then enters receiving chamber 112 through second exchange port 2112 and first receiving port 1121. Since the liquid-barrier vent membrane 130 allows gas to pass through, gas in receiving chamber 112 can be discharged through the liquid-barrier vent membrane 130 from second receiving port 1122, making the gas pressure in receiving chamber 112 equal to the external atmospheric pressure, ensuring that liquid in buffer chamber 111 smoothly enters receiving chamber 112.

[0039] When piston 120 moves away from exchange valve 200 in buffer chamber 111, i.e., when piston 120 moves upward, the pressure in buffer chamber 111 decreases. This allows liquid in receiving chamber 112 to enter exchange channel 211 through first receiving port 1121 and second exchange port 2112, and then enter buffer chamber 111 from first exchange port 2111 and first buffer port 1111. Since the liquid-barrier vent membrane 130 allows gas to pass through, external gas can enter receiving chamber 112 through the liquid-barrier vent membrane 130, making the gas pressure inside receiving chamber 112 equal to the external atmospheric pressure, ensuring that liquid in receiving chamber 112 smoothly enters buffer chamber 111.

[0040] See Figure 2 , Figure 3 , Figure 7 and Figure 8 Therefore, during the rotation of the valve assembly 210, when the second exchange port 2112 is connected to the first receiving port 1121 of different receiving chambers 112, the upward or downward movement of the piston 120 relative to the buffer chamber 111 can effectively realize the transfer of liquid between the receiving chamber 112 and the buffer chamber 111. It can be understood that when the second exchange port 2112 is connected to the first receiving port 1121 of one of the receiving chambers 112, the valve assembly 210 can block the first receiving ports 1121 of other receiving chambers 112, preventing liquid in other receiving chambers 112 from flowing out of the first receiving port 1121.

[0041] See Figure 2 , Figure 3 , Figure 7 and Figure 8In some embodiments, both the first buffer port 1111 and the first accommodating port 1121 are located above the exchange channel 211, which allows both the first buffer port 1111 and the first accommodating port 1121 to be located above the first exchange port 2111 and the second exchange port 2112. During the rotation of the valve assembly 210, the second exchange port 2112 can rotate around the center of the first exchange port 2111. The orthographic projection of the first accommodating port 1121 of different accommodating cavities along the axial direction of the housing assembly 110 can be located on the circumference formed by the rotation of the second exchange port 2112. It can also be understood that the first accommodating ports 1121 are spaced apart along the same circumference on the housing assembly 110. Therefore, during the rotation of the valve assembly 210, when the second exchange port 2112 is located below and aligned with the first accommodating port 1121 of one of the accommodating chambers 112, the exchange channel 211 can communicate with the accommodating chamber 112 through the second exchange port 2112, that is, the buffer chamber 111 can communicate with the accommodating chamber 112 through the exchange channel 211. Conversely, when the second exchange port 2112 is misaligned with the first accommodating port 1121 of the accommodating chamber 112, the exchange channel 211 cannot communicate with the accommodating chamber 112 through the second exchange port 2112, and thus the buffer chamber 111 can communicate with the accommodating chamber 112 through the exchange channel 211. Therefore, when the second exchange port 2112 is alternately located below different first accommodating ports 1121 and aligned with each other, the buffer cavity 111 can be alternately connected to different accommodating cavities 112 through the exchange channel 211, thereby realizing the transfer of liquid between different accommodating cavities 112 through the buffer cavity 111. It can be understood that when the second exchange port 2112 is aligned with one of the first accommodating ports 1121, the other first accommodating ports 1121 are misaligned with the second exchange port 2112 rather than aligned.

[0042] The accommodating chamber 112 can be used to hold samples or reagents, and multiple accommodating chambers 112 may include a sample chamber, a lysis buffer chamber, a first washing buffer chamber, a second washing buffer chamber, an elution buffer chamber, and an extraction chamber. The sample chamber is used to hold samples; however, the sample chamber can also hold waste liquid generated during processing, allowing it to be used as a waste liquid chamber—that is, the sample chamber and waste liquid chamber are shared. The lysis buffer chamber holds the lysis buffer, the first washing buffer chamber holds the first washing buffer, the second washing buffer chamber holds the second washing buffer, and the elution buffer chamber holds the elution buffer, which can be enzyme-free water, etc. The extraction chamber can contain lyophilized magnetic beads.

[0043] See Figure 2 , Figure 3 , Figure 7 and Figure 8 During the extraction of nucleic acids from the sample by the processing device 10, the following steps can be performed:

[0044] The first step is to add various reagents into different cavities 112 and add the sample to be processed into the sample cavity.

[0045] In the second step, by rotating the valve assembly 210, the second exchange port 2112 is positioned below the sample chamber, that is, below and aligned with the first receiving port 1121 of the sample chamber. At this time, the piston 120 moves upward, drawing the sample from the sample chamber into the buffer chamber 111 through the exchange channel 211. Then, the valve assembly 210 is moved so that the second exchange port 2112 is positioned below the lysis buffer chamber, and the piston 120 moves upward, drawing the lysis buffer from the lysis buffer chamber into the buffer chamber 111 containing the sample. At this time, the lysis buffer chamber and the sample form a mixture in the buffer chamber 111. Next, the valve assembly 210 is moved so that the second exchange port 2112 is positioned below the extraction chamber, and the piston 120 moves downward, injecting the mixture formed by the lysis buffer chamber and the sample in the buffer chamber 111 into the extraction chamber through the exchange channel 211. Of course, the extraction chamber is already filled with lyophilized magnetic beads.

[0046] The third step involves using an external ultrasonic device to contact the shell assembly 110 at the position corresponding to the extraction cavity. Under the action of ultrasound, the cells in the sample undergo material rupture through the lysis buffer, thereby releasing the nucleic acid in the cells and achieving nucleic acid lysis.

[0047] Fourth, by rotating the valve assembly 210, the second exchange port 2112 is positioned below the binding fluid chamber. The piston 120 moves upward, drawing the binding fluid from the binding fluid chamber into the buffer chamber 111 through the exchange channel 211. Then, by rotating the valve assembly 210, the second exchange port 2112 is positioned below the extraction chamber. The piston 120 moves downward, injecting the binding fluid from the buffer chamber 111 into the extraction chamber through the exchange channel 211.

[0048] The fifth step involves using an external ultrasonic device to contact the shell assembly 110 at the position corresponding to the extraction chamber. Under the action of ultrasound, the nucleic acid in the liquid is thoroughly mixed and incubated with the lyophilized magnetic beads, ensuring that all the nucleic acid in the liquid is adsorbed onto the lyophilized magnetic beads, thus making the lyophilized magnetic beads a carrier for the nucleic acid. Obviously, when all the nucleic acid is adsorbed onto the lyophilized magnetic beads, the liquid in the extraction chamber will become waste liquid because it no longer contains nucleic acid.

[0049] The sixth step involves using an external magnet to attach the lyophilized magnetic beads containing nucleic acids to the inner wall of the extraction chamber via the magnetic attraction force generated by the external magnet, thus preventing the lyophilized magnetic beads containing nucleic acids from falling off the inner wall.

[0050] Step 7: Continue to attach the external magnet to the shell assembly 110, ensuring that the lyophilized magnetic beads with adsorbed nucleic acids are adsorbed onto the inner wall of the extraction chamber. Since the second exchange port 2112 is still located below the extraction chamber, the piston 120 can move upwards, allowing the waste liquid in the extraction chamber to be drawn into the buffer chamber 111 through the exchange channel 211. It can be understood that during the process of drawing the waste liquid into the buffer chamber 111, the lyophilized magnetic beads are adsorbed onto the inner wall of the extraction chamber, preventing the lyophilized magnetic beads with adsorbed nucleic acids from being drawn into the buffer chamber 111, effectively avoiding loss of the lyophilized magnetic beads. Then, by rotating the valve assembly 210, the second exchange port 2112 is positioned below the sample chamber, i.e., below the waste liquid chamber. The piston 120 can then move downwards, injecting the waste liquid in the buffer chamber 111 into the sample chamber through the exchange channel 211. At this point, there is no liquid in the extraction chamber, only the lyophilized magnetic beads with adsorbed nucleic acids, thus achieving nucleic acid binding.

[0051] In the eighth step, by rotating the valve assembly 210, the second exchange port 2112 is positioned below the first cleaning fluid chamber, causing the piston 120 to move upward and draw the first cleaning fluid from the first cleaning fluid chamber into the buffer chamber 111. Then, rotating the valve assembly 210, the second exchange port 2112 is positioned below the extraction chamber, causing the piston 120 to move downward and inject the first cleaning fluid from the buffer chamber 111 into the extraction chamber. The external magnet is removed, so that the lyophilized magnetic beads adsorbed with nucleic acids will no longer adhere to the inner wall of the extraction chamber and will enter the first cleaning fluid. Then, by contacting the shell assembly 110 with the position corresponding to the extraction chamber using an external ultrasonic device, the lyophilized magnetic beads adsorbed with nucleic acids and the first cleaning fluid are mixed under the action of ultrasound, allowing the first cleaning fluid to clean the lyophilized magnetic beads and further remove impurities from the lyophilized magnetic beads. After cleaning, the lyophilized magnetic beads adsorbed with nucleic acid are attracted to the shell assembly 110 by an external magnet, ensuring that they are re-adsorbed onto the inner wall of the extraction chamber. This causes the piston 120 to move upward, drawing the waste liquid generated from the first cleaning solution into the buffer chamber 111. It can be understood that the lyophilized magnetic beads adsorbed with nucleic acid are retained in the extraction chamber because they are adsorbed onto the inner wall, preventing loss of the lyophilized magnetic beads. Then, the valve assembly 210 is rotated so that the second exchange port 2112 is located below the sample chamber, causing the piston 120 to move downward, injecting the waste liquid from the buffer chamber 111 into the sample chamber. At this point, the extraction chamber contains no liquid, only the lyophilized magnetic beads adsorbed with nucleic acid.

[0052] Step nine can be repeated multiple times from step eight, allowing the first cleaning solution to clean the lyophilized magnetic beads multiple times, thereby improving the cleaning effect on impurities. Of course, if step eight only needs to be performed once, step nine can be omitted, and the following step ten can be performed directly.

[0053] Step 10: By rotating valve assembly 210, the second exchange port 2112 is positioned below the second cleaning fluid chamber, causing piston 120 to move upward and draw the second cleaning fluid from the second cleaning fluid chamber into buffer chamber 111. Then, rotating valve assembly 210, the second exchange port 2112 is positioned below the extraction chamber, causing piston 120 to move downward and inject the second cleaning fluid from buffer chamber 111 into the extraction chamber. The external magnet is removed, so the lyophilized magnetic beads adsorbed with nucleic acid will no longer adhere to the inner wall of the extraction chamber and will enter the second cleaning fluid. Then, the external ultrasonic device contacts the shell assembly 110 at the position corresponding to the extraction chamber, causing the lyophilized magnetic beads adsorbed with nucleic acid to mix with the second cleaning fluid under ultrasonic action, allowing the second cleaning fluid to clean the lyophilized magnetic beads and further remove impurities from them. After cleaning, the lyophilized magnetic beads adsorbed with nucleic acid are attracted to the shell assembly 110 by an external magnet, ensuring that they are re-adsorbed onto the inner wall of the extraction chamber. This causes the piston 120 to move upward, drawing the waste liquid generated from the second cleaning solution into the buffer chamber 111. It can be understood that the lyophilized magnetic beads adsorbed with nucleic acid are retained in the extraction chamber because they are adsorbed onto the inner wall, preventing loss of the lyophilized magnetic beads. Then, the valve assembly 210 is rotated so that the second exchange port 2112 is located below the sample chamber, causing the piston 120 to move downward, injecting the waste liquid from the buffer chamber 111 into the sample chamber. At this point, the extraction chamber contains no liquid, only the lyophilized magnetic beads adsorbed with nucleic acid.

[0054] Step 11 can be repeated multiple times, allowing the second washing solution to repeatedly wash the lyophilized magnetic beads, thereby improving the cleaning effect on impurities and achieving nucleic acid cleaning. Alternatively, if step 10 only needs to be performed once, it can be omitted, and the process can proceed directly to step 12.

[0055] In the twelfth step, by rotating the valve assembly 210, the second exchange port 2112 is positioned below the eluent chamber, causing the piston 120 to move upward and draw the eluent from the eluent chamber into the buffer chamber 111. Then, by rotating the valve assembly 210, the second exchange port 2112 is positioned below the extraction chamber, causing the piston 120 to move downward and inject the eluent from the buffer chamber 111 into the extraction chamber.

[0056] In the thirteenth step, an external ultrasonic device and a heating device are used to alternately contact the shell assembly 110 with the corresponding position of the extraction chamber. Under the action of ultrasound and heat, the lyophilized magnetic beads adsorbed with nucleic acid will be fully mixed and incubated with the elution solution, so that the nucleic acid is eluted from the lyophilized magnetic beads, that is, the nucleic acid is separated from the lyophilized magnetic beads and incorporated into the elution solution. The elution solution containing nucleic acid will form nucleic acid products, thus realizing the elution of nucleic acid.

[0057] In the fourteenth step, an external magnet is used to adhere the lyophilized magnetic beads, which no longer have nucleic acid adsorbed, to the inner wall of the extraction chamber. Then, the piston 120 moves upward, drawing the nucleic acid product from the extraction chamber into the buffer chamber 111. This completes the extraction of nucleic acid from the sample.

[0058] Therefore, on the one hand, the rotation of the valve assembly 210 allows the buffer chamber 111 to alternately connect with different receiving chambers 112 through the same exchange channel 211, enabling liquid exchange between the buffer chamber 111 and different receiving chambers 112. Ultimately, the liquid in different receiving chambers 112 is exchanged through the buffer chamber 111, avoiding the use of multiple pipes for liquid exchange, thus simplifying the structure of the processing device 10, reducing manufacturing costs, and improving reliability. On the other hand, the liquid-sealing and breathable membrane 130 prevents aerosols and liquids in the receiving chambers 112 from leaking outside the processing device 10, and also prevents external impurities from entering the buffer chambers 111 or receiving chambers 112, thus avoiding cross-contamination. Furthermore, liquid transfer within the same processing device 10 avoids transfer between different devices, reducing transfer distance and time, thereby improving the working efficiency of the processing device 10. Simultaneously, liquid transfer can be automated by controlling the movement of the exchange valve 200 and piston 120, avoiding manual intervention and further improving the working efficiency of the processing device 10. It is understandable, given that human intervention can be avoided during the liquid transfer process, and cross-contamination between the liquid and the human body can also be avoided.

[0059] See Figure 4 , Figure 5 and Figure 6 In some embodiments, the processing device 10 may further include a collection tube 400 connected to the housing assembly 110. The housing assembly 110 also has a collection chamber 114, with multiple accommodating chambers 112 and the collection chamber 114 spaced around the buffer chamber 111. The collection chamber 114 can communicate with the lumen of the collection tube 400, and the liquid-barrier breathable membrane 130 can also seal the opening of the collection chamber 114 away from the exchange valve 200. When the valve assembly 210 rotates, the second exchange port 2112 can be located below the collection chamber 114 and aligned with it, so that the second exchange port 2112 and the collection chamber 114 communicate with each other, thereby allowing liquid in the buffer chamber 111 to enter the collection chamber 114.

[0060] Therefore, when the processing device 10 also includes a collection tube 400, after the fourteenth step described above is completed, the valve assembly 210 can be rotated so that the second exchange port 2112 is located below the collection chamber 114. Then, the piston 120 moves downward, thereby injecting the nucleic acid product in the buffer chamber 111 into the lumen of the collection tube 400 through the exchange channel 211 and the collection chamber 114. This achieves the storage of nucleic acid products by the collection tube 400. This enables the processing device 10 to automatically store and collect nucleic acid products, effectively avoiding the inability of the processing device 10 to automatically store and collect nucleic acid products, thereby improving the versatility of the processing device 10's functions and ultimately enhancing its applicability to various operating conditions.

[0061] See Figure 1 , Figure 4 , Figure 5 and Figure 6 In some embodiments, the housing assembly 110 includes a housing 117 and a cover 118, with the cover 118 connected to the end of the housing 117 away from the exchange valve 200. The housing 117 includes a base plate 1171, an inner cylinder 1172, an outer cylinder 1173, and a partition 1174, with both the inner cylinder 1172 and the outer cylinder 1173 protruding along the thickness direction of the base plate 1171. An outer cylinder 1173 surrounds an inner cylinder 1172. A partition 1174 is located in the space between the inner cylinder 1172 and the outer cylinder 1173. The partition 1174 is connected to and / or to the inner cylinder 1172. Alternatively, the lower end of the partition 1174 can be connected to the bottom plate 1171. This allows the space between the partitions 1174 to be configured as part of the receiving cavity 112 or the collecting cavity 114, and the space enclosed by the inner cylinder 1172 to be configured as part of the buffer cavity 111. A second buffer port 1112 and a second receiving port 1122 are located on the cover 118. This allows the buffer cavity 111 and the receiving cavity 112 to be integrated on the same housing assembly 110, thereby simplifying the structure of the processing device 10.

[0062] See Figure 1 , Figure 4 , Figure 5 and Figure 6In some embodiments, the cover 118 includes a first cover 1181 and a second cover 1182. The first cover 1181 is fixedly connected to the housing 117, and the second cover 1182 is movably connected to the first cover 1181 to open or close the first cover 1181. The first cover 1181 and the second cover 1182 are provided with a buffer hole 1183 and a receiving hole 1184. The buffer hole 1183 is configured as part of the buffer cavity 111, and its opening on the second cover 1182 is a second buffer opening 1112. The receiving hole 1184 is configured as part of the receiving cavity 112, and its opening on the second cover 1182 is a second receiving opening 1122. A liquid-blocking and breathable membrane 130 is located between the first cover 1181 and the second cover 1182, enabling it to seal the buffer hole 1183 and the receiving hole 1184, thereby sealing the second buffer port 1112 and the second receiving port 1122. The liquid-blocking and breathable membrane 130 can be fixedly connected to the second cover 1182. When the second cover 1182 opens the first cover 1181, interference from the liquid-blocking and breathable membrane 130 is avoided, facilitating the addition of liquid to the receiving cavity 112 through the receiving hole 1184 on the first cover 1181. When the second cover 1182 closes the first cover 1181, the liquid-blocking and breathable membrane 130 seals the buffer hole 1183 and the receiving hole 1184. This improves the ease of operation of the processing device 10.

[0063] See Figure 1 , Figure 4 , Figure 5 and Figure 6 In some embodiments, the second cover 1182 includes a rotating portion 1182a and a connecting portion 1182b. The rotating portion 1182a is rotatably connected to the first cover 1181, and the connecting portion 1182b is detachably connected to the first cover 1181, for example, by a snap-fit ​​connection. When it is necessary to open the first cover 1181, the snap-fit ​​connection between the connecting portion 1182b and the first cover 1181 can be released, allowing the connecting portion 1182b to rotate away from the first cover 1181. When it is necessary to close the first cover 1181, the connecting portion 1182b can rotate closer to the first cover 1181 until it contacts the first cover 1181 and forms a snap-fit ​​connection. In other embodiments, the second cover 1182 can be slidably connected to the first cover 1181, which also allows the second cover 1182 to open or close the first cover 1181.

[0064] See Figure 2 , Figure 6 and Figure 7In some embodiments, the housing assembly 110 further includes a seal 119, which is connected to the housing 117 and abuts against the valve assembly 210 and the base plate 1171. The first buffer port 1111 and the first receiving port 1121 are located within the seal 119. By providing the seal 119, which has a certain degree of flexibility, the seal 119 can effectively eliminate the gap between it and the valve assembly 210 when it abuts against the valve assembly 210. When the second exchange port 2112 communicates with the first receiving port 1121 of one of the receiving cavities 112, the valve assembly 210 can effectively seal the first receiving ports 1121 of the other receiving cavities 112, thereby preventing leakage of liquid from the other receiving cavities 112 through the first receiving port 1121. This improves the reliability of the processing device 10.

[0065] See Figure 1 In some embodiments, the outer circumferential surface of the outer cylinder 1173 of the shell assembly 110 includes a plurality of planes 1175. These planes 1175 are spaced apart circumferentially along the outer cylinder 1173, and different planes 1175 correspond to the receiving cavity 112. By providing the planes 1175, it is easier for the external ultrasonic device to contact the shell assembly 110, preventing the external ultrasonic device from slipping relative to the shell assembly 110. It also facilitates the installation of the external magnet, preventing the external magnet from slipping relative to the shell assembly 110.

[0066] See Figure 3 , Figure 8 , Figure 9 and Figure 10In some embodiments, the valve assembly 210 includes a drive member 213 and a valve body 214. The valve body 214 can be stacked on the drive member 213, such that the valve body 214 can abut between the drive member 213 and the seal 119. An exchange groove 2114 is recessed on the surface of the drive member 213 facing the seal 119. The exchange groove 2114 can be curved. The valve body 214 connects to the drive member 213 and seals the opening of the exchange groove 2114. The valve body 214 is provided with a first exchange hole 2115 and a second exchange hole 2116, which penetrate the valve body 214 along its thickness direction. The first exchange hole 2115 and the second exchange hole 2116 are spaced apart and both communicate with the exchange groove 2114. The first exchange hole 2115 has an opening on the surface of the valve body 214 facing the seal 119, and this opening is the first exchange port 2111; the second exchange hole 2116 has an opening on the surface of the valve body 214 facing the seal 119, and this opening is the second exchange port 2112. The exchange groove 2114, the first exchange hole 2115, and the second exchange hole 2116 are configured as an exchange channel 211, such that the exchange channel 211 includes the exchange groove 2114, the first exchange hole 2115, and the second exchange hole 2116.

[0067] See Figure 3 , Figure 8 , Figure 9 and Figure 10 In some embodiments, the exchange valve 200 further includes a carrier 220, which is detachably connected to the housing assembly 110. For example, the carrier 220 can be snap-fitted to the outer cylinder 1173. The drive member 213 abuts between the carrier 220 and the valve body 214, and the valve body 214 abuts against the seal 119. This allows the entire valve assembly 210 to be abutted between the carrier 220 and the seal 119, thereby enabling the installation of the valve assembly 210 and preventing it from sliding along the axial direction of the housing assembly 110. This ensures that the valve assembly 210 can only rotate about an axis extending axially along the housing assembly 110.

[0068] See Figure 6 In some embodiments, the buffer cavity 111 has a constriction section located near the first buffer port 1111, i.e., near the seal 119. The diameter of the constriction section decreases from the second buffer port 1112 towards the first buffer port 1111; that is, the diameter gradually decreases from top to bottom, thus the constriction section is approximately conical. This allows the constriction section to guide and converge the liquid in the buffer cavity 111, ensuring that all liquid is discharged from the buffer cavity 111 during the downward movement of the piston 120, preventing liquid residue from remaining in the buffer cavity 111.

[0069] See Figure 4 and Figure 5 In some embodiments, the cover 118 further includes a shielding membrane 1185. A connecting groove 1181a is provided on the first cover 1181. The connecting groove 1181a is formed by recessing the surface of the first cover 1181 toward the second cover 1182 to a certain depth. The connecting groove 1181a can connect the collection chamber 114 and the lumen of the collection tube 400. The shielding membrane 1185 is used to seal the opening of the connecting groove 1181a, thus making the connecting groove 1181a a closed channel. When it is necessary to automatically collect the nucleic acid products extracted from the buffer chamber 111, the valve assembly 210 can be rotated so that the second exchange port 2112 is located below the collection chamber 114, and then the piston 120 moves downward, thereby injecting the nucleic acid products in the buffer chamber 111 into the lumen of the collection tube 400 through the exchange channel 211, the collection chamber 114 and the connecting groove 1181a in sequence, thus realizing the collection of nucleic acid products by the collection tube 400.

[0070] See Figure 4 and Figure 5 In some embodiments, the first cover 1181 may further include a protrusion 1181b, which may be generally columnar in shape. When the shielding membrane 1185 is connected to the first cover 1181, the protrusion 1181b can be inserted into the shielding membrane 1185, thereby enabling the shielding membrane 1185 to be positioned and improving the installation accuracy and connection strength of the shielding membrane 1185. Of course, the protrusion 1181b can also be inserted into the liquid-proof and breathable membrane 130, which can also provide good positioning for the liquid-proof and breathable membrane 130. The protrusions 1181b inserted into the shielding membrane 1185 and the liquid-proof and breathable membrane 130 may be different or the same.

[0071] See Figure 4 and Figure 5 In some embodiments, the cover 118 of the shell assembly 110 is further provided with a vent 1186, which connects the lumen of the collection tube 400 to the outside. By providing the vent 1186, when liquids such as nucleic acid products enter the lumen of the collection tube 400 from the collection chamber 114 and the connecting groove 1181a, the gas in the lumen of the collection tube 400 can be discharged to the outside through the vent 1186, so that the gas pressure inside the collection tube 400 is equivalent to the outside atmospheric pressure, ensuring that liquids such as nucleic acid products can smoothly enter the lumen of the collection tube 400.

[0072] See Figure 2 , Figure 3 and Figure 11In some embodiments, the processing device 10 further includes a reaction mechanism 300, which is detachably connected to the outer cylinder 1173 of the shell assembly 110, for example, by snap-fit ​​connection. The reaction mechanism 300 has a reaction chamber 330 and a first reaction channel 310, which communicate with the outside. The shell assembly 110 has a first flow channel 115, and the first reaction channel 310 and the first flow channel 115 are always in communication. When the valve assembly 210 rotates, the second exchange port 2112 can communicate with the first flow channel 115 and different accommodating chambers 112. Therefore, when the second exchange port 2112 is located below the first flow channel 115, the second exchange port 2112 is aligned with the end opening of the first flow channel 115, thereby allowing the second exchange port 2112 to communicate with the reaction chamber 330 through the first flow channel 115 and the first reaction channel 310. The multiple cavities 112 may also include a PCR reagent cavity, which is used to hold PCR reagents.

[0073] See Figure 2 , Figure 3 and Figure 11 After the nucleic acid product in the extraction chamber is drawn into the buffer chamber 111 through the fourteenth step described above, if it is not necessary to input the nucleic acid product into the collection tube 400 for storage through the collection chamber 114, the nucleic acid can be input into the reaction chamber 330 for amplification reaction in order to perform subsequent fluorescence detection. The operation steps are as follows:

[0074] First, rotate valve assembly 210 to align the second exchange port 2112 with the PCR reagent chamber, thus establishing communication between the exchange channel 211 and the PCR reagent chamber. Drive piston 120 downwards, introducing nucleic acid products from buffer chamber 111 into the PCR reagent chamber through exchange channel 211, allowing the nucleic acid products to mix with the PCR reagents in the PCR reagent chamber. To ensure uniform mixing, piston 120 can be moved up and down repeatedly to create turbulence and thorough mixing of the nucleic acid products and PCR reagents. After mixing is complete, piston 120 can be driven upwards to draw the mixture of nucleic acid products and PCR reagents from the PCR reagent chamber into buffer chamber 111.

[0075] Then, the valve assembly 210 is rotated so that the second exchange port 2112 is aligned with the first guide channel 115, thus connecting the exchange channel 211 and the first guide channel 115. The drive piston 120 moves downward, feeding the mixture in the buffer chamber 111 into the reaction chamber 330 through the exchange channel 211, the first guide channel 115 and the first reaction channel 310 in sequence.

[0076] Finally, insert the reaction mechanism 300 into the external PCR device, start the amplification program, and perform fluorescence detection after the amplification is complete.

[0077] Therefore, by setting up the reaction mechanism 300, after the nucleic acid product extraction is completed, the nucleic acid product can be directly input into the reaction mechanism 300 for amplification reaction to achieve fluorescence detection. This allows nucleic acid extraction and detection to be completed on the same processing device 10, meaning that the processing device 10 simultaneously has nucleic acid extraction and detection functions. This also improves the versatility of the processing device 10's functions and ultimately enhances its applicability to various operating conditions. It can be understood that the extracted nucleic acid product can directly enter the reaction mechanism 300 for reaction and fluorescence detection without needing to remove the nucleic acid product from the processing device 10 to transfer it to other detection devices for fluorescence detection. The transfer of the nucleic acid product only needs to be completed within the processing device 10. This reduces the transfer distance and time of the nucleic acid product, thereby improving the working efficiency of the processing device 10. Of course, the transfer of the nucleic acid product is completed within the closed processing device 10, which effectively avoids contamination of the nucleic acid product between the processing device 10 and external detection devices, thus providing reliability to the processing device 10.

[0078] See Figure 2 , Figure 3 and Figure 11 In some embodiments, the shell assembly 110 also has a second flow channel 116 and a venting cavity 113, which are spaced apart. The venting cavity 113 penetrates the cover 118, forming an opening on the cover 118 that connects to the outside. The liquid-proof and breathable membrane 130 can seal this opening. The reaction mechanism 300 also has a second reaction channel 320, which is always in communication with the reaction chamber 330 and the second flow channel 116. When the second exchange port 2112 is connected to the first flow channel 115, the venting cavity 113 can be connected to the second flow channel 116. For example, the valve assembly 210 also has a transfer channel 212, which is independent of the exchange channel 211 and is not connected to it. The transfer channel 212 has a first transfer port 2121 and a second transfer port 2122, which are spaced apart. When the second exchange port 2112 is connected to the first guide channel 115, the first transfer port 2121 is connected to the ventilation chamber 113, and the second transfer port 2122 is connected to the second guide channel 116. This allows the ventilation chamber 113 to be interconnected with the second guide channel 116 via the transfer channel 212.

[0079] Therefore, when the second exchange port 2112 is connected to the first flow channel 115, on the one hand, the buffer chamber 111 is connected to the reaction chamber 330 in sequence through the exchange channel 211, the first flow channel 115 and the first reaction channel 310; on the other hand, the ventilation chamber 113 is connected to the reaction chamber 330 in sequence through the transfer channel 212, the second flow channel 116 and the second reaction channel 320. In this way, the reaction chamber 330 can be connected to the outside through the second reaction channel 320, the second flow channel 116, the transfer channel 212 and the ventilation chamber 113. That is, outside gas can enter the reaction chamber 330 through the ventilation chamber 113, so that the gas pressure in the reaction chamber 330 near the second reaction channel 320 is equivalent to atmospheric pressure. Therefore, when the liquid in the buffer chamber 111 enters the reaction chamber 330, the gas in the reaction chamber 330 can be discharged to the outside through the second reaction channel 320, the second guide channel 116, the transfer channel 212, and the venting chamber 113 in sequence, avoiding an increase in the gas pressure in the reaction chamber 330 and ensuring that the liquid in the buffer chamber 111 smoothly enters the reaction chamber 330. Of course, when the second exchange port 2112 is misaligned with the first guide channel 115 and is not connected to each other, the first transfer port 2121 is misaligned with the venting chamber 113 and is not connected to each other, and the second transfer port 2122 is misaligned with the second guide channel 116 and is not connected to each other.

[0080] It is understood that during the amplification reaction of the liquid in the reaction mechanism 300, the second exchange port 2112 can be misaligned with the first guide channel 115 and thus not connected to each other, the first transfer port 2121 can be misaligned with the venting chamber 113 and thus not connected to each other, and the second transfer port 2122 can be misaligned with the second guide channel 116 and thus not connected to each other. This allows the sealing element 119 to block the end openings of the first guide channel 115 and the second guide channel 116, thereby preventing the reaction chamber 330 from connecting to the outside world and the buffer chamber 111, preventing liquid leakage in the reaction chamber 330, and improving the reliability of the amplification reaction.

[0081] In other embodiments, in order to connect the reaction chamber 330 to the outside so that the buffer chamber 111 can smoothly inject liquid into the reaction chamber 330, an air passage connecting the reaction chamber 330 can be directly opened on the reaction mechanism 300, so that the air passage connects to the outside. In this way, the gas in the reaction chamber 330 can also be discharged through the air passage, ensuring that the buffer chamber 111 can smoothly inject liquid into the reaction chamber 330.

[0082] See Figure 8 , Figure 9 , Figure 10 and Figure 11In some embodiments, the drive member 213 has a transfer groove 2123. The valve body 214 is connected to the drive member 213 and seals the opening of the transfer groove 2123. The valve body 214 has a first transfer hole 2124 and a second transfer hole 2125, which are spaced apart and connected to the transfer groove 2123. The transfer groove 2123, the first transfer hole 2124, and the second transfer hole 2125 are configured as a transfer channel 212, that is, the transfer channel 212 includes the transfer groove 2123, the first transfer hole 2124, and the second transfer hole 2125. The first transfer port 2121 is located in the first transfer hole 2124, and the second transfer port 2122 is located in the second transfer hole 2125. In other embodiments, the first transfer hole 2124 and the second transfer hole 2125 can overlap to form the same hole, so that the venting cavity 113 and the second flow channel 116 are connected to the transfer groove 2123 through the same hole, that is, the venting cavity 113 and the second flow channel 116 are connected to the transfer channel 212 through the same position on the transfer channel 212.

[0083] See Figure 8 , Figure 9 , Figure 10 and Figure 11 In some embodiments, the exchange channel 211 also has a pressurization port 2113, which is spaced apart from the first exchange port 2111 and the second exchange port 2112. For example, the pressurization port 2113 is spaced apart from the second exchange port 2112 on the circumference formed by the rotation of the second exchange port 2112. When the valve assembly 210 rotates, the pressurization port 2113 can communicate with the first guide channel 115. At this time, the second exchange port 2112 is misaligned with the first guide channel 115, and the sealing member 119 will block the venting chamber 113, the second guide channel 116, the second exchange port 2112, the first transfer port 2121 and the second transfer port 2122. The gas in the reaction chamber 330 will not be able to be discharged to the outside through the venting chamber 113. When the piston 120 is slid downward, the gas in the buffer chamber 111 can enter the reaction chamber 330 through the pressurization port 2113 of the exchange channel 211, the first guide channel 115 and the first reaction channel 310 in sequence. Since the gas in the reaction chamber 330 cannot be discharged to the outside through the venting chamber 113, the gas pressure in the reaction chamber 330 increases.

[0084] See Figure 3 and Figure 11In some embodiments, the reaction mechanism 300 includes a reaction plate 340 and a covering membrane 350. The reaction plate 340 is connected to the shell assembly 110. Both the first reaction channel 310 and the second reaction channel 320 can be disposed inside the reaction plate 340. The reaction cavity 330 extends through the reaction plate 340 along its thickness direction. Two covering membranes 350 are located on opposite sides of the reaction plate 340 in the thickness direction, such that the reaction cavity 330 is located between the two covering membranes 350. This allows the two covering membranes 350 to seal the two openings of the reaction cavity 330 in the thickness direction of the reaction plate 340, thereby sealing the reaction cavity 330.

[0085] When the liquid undergoes an amplification reaction in the reaction chamber 330, an external heating element can be attached to the cover film 350 and the reaction plate 340 to allow the liquid to undergo the amplification reaction at a certain temperature. Given the relatively small thickness of the reaction plate 340 and the cover film 350, the heat conduction speed of the reaction plate 340 and the cover film 350 can be increased. This allows heat from the heating element to be quickly conducted through the reaction plate 340 and the cover film 350 to the liquid inside the reaction chamber 330, thereby increasing the heat conduction speed and reducing heat damage. This increases the speed of the amplification reaction, thus improving the working efficiency of the processing device 10.

[0086] During the heating process via the heating element, the pressurization port 2113 can be connected to the first flow channel 115, causing the piston 120 to move downwards. The gas in the buffer chamber 111 enters the reaction chamber 330, increasing the gas pressure in the reaction chamber 330. This causes the covering films 350 on both sides of the reaction plate 340 to expand outwards, ensuring that the covering films 350 are tightly attached to the heating element. This ensures that the heat from the heating element is quickly transferred through the covering films 350 to the liquid in the reaction chamber 330, thereby further improving the speed of the amplification reaction and the working efficiency of the processing device 10.

[0087] In some embodiments, the reaction mechanism 300 further includes a connector 360, which is detachably connected to the shell assembly 110, for example, by snap-fitting the connector 360 to the shell assembly 110. The reaction plate 340 passes through the connector 360 and is fixedly connected to the connector 360. Alternatively, the reaction mechanism 300 may also include a sealing ring, which abuts against the reaction plate 340 and the shell assembly 110, thereby sealing the connection between the first reaction channel 310 and the first flow channel 115, and also sealing the connection between the second reaction channel 320 and the second flow channel 116.

[0088] See Figure 8 , Figure 9 , Figure 10 and Figure 11In some embodiments, the number of reaction mechanisms 300 can be two, and the two reaction mechanisms 300 can be symmetrically arranged relative to the shell assembly 110. In addition to performing amplification reactions for fluorescence detection, the reaction mechanisms 300 can also perform library construction through diffusion reactions. Therefore, the processing device 10 can integrate multiple functions such as nucleic acid extraction, nucleic acid detection, and library construction, thereby further improving the functional diversity of the processing device 10 and its applicability to various operating conditions. When the processing device 10 performs library construction, it can execute NGS, tNGS, and third-generation sequencing library construction processes; that is, the processing device 10 is compatible with second-generation sequencing library construction processes represented by NGS and tNGS, and also compatible with third-generation sequencing library construction processes.

[0089] The multiple cavities 112 may also include a first PCR reagent cavity, a second PCR reagent cavity, a first purification cavity, a second purification cavity, and an ethanol cavity, etc. The following is a brief explanation using the tNGS library construction process:

[0090] After the nucleic acid product is transferred to the buffer chamber 111 in the fourteenth step of the nucleic acid extraction process, the following operations can be performed sequentially by rotating the valve assembly 210 and moving the piston 120 up and down: transferring the nucleic acid product from the buffer chamber 111 to the first PCR reagent chamber for mixing; transferring the nucleic acid product mixed with the first PCR reagent to the buffer chamber 111; transferring the nucleic acid product mixed with the first PCR reagent from the buffer chamber 111 to the reaction chamber 330 of one of the reaction mechanisms 300 for amplification reaction; and transferring the amplification product in the reaction chamber 330 to the buffer chamber 111. The amplification product is transferred to the first purification chamber, the waste liquid is transferred from the first purification chamber to the buffer chamber 111, the waste liquid is transferred from the buffer chamber 111 to the sample chamber, the ethanol is transferred from the ethanol chamber to the buffer chamber 111, the ethanol is transferred from the buffer chamber 111 to the first purification chamber, the waste liquid is transferred from the first purification chamber to the buffer chamber 111, the waste liquid is transferred from the buffer chamber 111 to the sample chamber, the ethanol is transferred from the ethanol chamber to the buffer chamber 111, the ethanol is transferred from the buffer chamber 111 to the first purification chamber, the waste liquid is transferred from the first purification chamber to the buffer chamber 111, and the waste liquid is transferred from the buffer chamber 111 to the sample chamber. The elution buffer is transferred from the elution chamber to the buffer chamber 111, the elution buffer is transferred from the buffer chamber 111 to the first purification chamber, the elution product is transferred from the first purification chamber to the buffer chamber 111, the elution product is transferred from the buffer chamber 111 to the second PCR reagent chamber, the elution product mixed with the second PCR reagent is transferred from the second PCR reagent chamber to the buffer chamber 111, the elution product mixed with the second PCR reagent is transferred from the buffer chamber 111 to the reaction chamber 330 of another reaction mechanism 300 for amplification reaction, the amplification product is transferred from the reaction chamber 330 to the buffer chamber 111, the amplification product is transferred from the buffer chamber 111 to the second purification chamber, the waste liquid is transferred from the second purification chamber to the buffer chamber 111, the waste liquid is transferred from the buffer chamber 111 to the sample chamber, the ethanol is transferred from the ethanol chamber to the buffer chamber 111, the ethanol is transferred from the buffer chamber 111 to the second purification chamber, the waste liquid is transferred from the second purification chamber to the buffer chamber 111, and the waste liquid is transferred from the buffer chamber 111 to the sample chamber. Ethanol is transferred from the ethanol chamber to the buffer chamber 111, then from the buffer chamber 111 to the second purification chamber. Waste liquid is transferred from the second purification chamber to the buffer chamber 111, and then from the buffer chamber 111 to the sample chamber. Enzyme-free water and other elution buffers are transferred from the elution chamber to the buffer chamber 111, then from the buffer chamber 111 to the second purification chamber. The elution product is then transferred from the second purification chamber to the buffer chamber 111. At this point, the elution product in the buffer chamber 111 is actually the library product. The library product is then transferred from the buffer chamber 111 to the collection tube 400 through the collection chamber 114.

[0091] Therefore, by equipping the processing device 10 with a collection tube 400 and a reaction mechanism 300, when the nucleic acid product is not amplified by the reaction mechanism 300 for fluorescence detection, the nucleic acid product can be input into the collection tube 400 for storage. The reaction mechanism 300 can amplify the nucleic acid product for fluorescence detection, and it can also amplify the nucleic acid product for library construction, allowing the library product to be collected through the collection tube 400. Thus, the collection tube 400 can collect both nucleic acid products and library products.

[0092] The technical features of the above embodiments can be combined in any way. For the sake of brevity, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0093] The embodiments described above are merely illustrative of several implementation methods of this application, and while the descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the patent application. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of this application, and these all fall within the protection scope of this application. Therefore, the protection scope of this patent application should be determined by the appended claims.

Claims

1. A processing device, characterized by, include: The host includes a shell assembly and a piston. The shell assembly has a buffer cavity and a plurality of receiving cavities that extend through the shell assembly along the axial direction of the host. The shell assembly also has a collection cavity. The plurality of receiving cavities and the collection cavity are arranged at intervals around the buffer cavity. The piston is slidably disposed within the buffer cavity. An exchange valve includes a valve assembly rotatably connected to the housing assembly. The valve assembly has an exchange channel with a first exchange port and a second exchange port spaced apart. When the valve assembly rotates, the first exchange port is always in communication with the buffer cavity, and the second exchange port is capable of communicating with the collection cavity and different accommodating cavities. A collection tube is connected to the shell assembly, and the collection cavity is in communication with the lumen of the collection tube.

2. The processing device of claim 1, wherein, The shell assembly is also provided with a ventilation hole, which connects the cavity of the collection tube to the outside.

3. The processing device of claim 1, wherein, The shell assembly includes a shell and a cover. The shell includes a bottom plate, an inner cylinder, an outer cylinder, and a partition. The inner cylinder and the outer cylinder both protrude from the bottom plate. The outer cylinder surrounds the inner cylinder. The partition is connected to the inner cylinder and / or the inner cylinder. The space enclosed by the inner cylinder is configured as part of the buffer cavity. The space between the partitions is configured as part of the receiving cavity and the collecting cavity.

4. The processing device of claim 3, wherein, The cover includes a first cover, a second cover, and a shielding membrane. The first cover is fixedly connected to the housing, and the second cover is movably connected to the first cover to open or close the first cover. The first cover and the second cover are provided with a buffer hole and a receiving hole. The buffer hole is configured as part of the buffer cavity, and the receiving hole is configured as part of the receiving cavity. A connecting groove is recessed on the surface of the first cover facing the second cover. The connecting groove connects the collecting cavity and the lumen of the collecting tube. The shielding membrane is used to seal the opening of the connecting groove.

5. The processing device of claim 1, wherein, The collection tube is detachably connected to the shell assembly.

6. The processing device of claim 1, wherein, It also includes a reaction mechanism, and the shell assembly is provided with a first flow channel. The reaction mechanism is detachably connected to the shell assembly and is provided with a reaction chamber and a first reaction channel. The reaction chamber can connect the first reaction channel to the outside. The first reaction channel is connected to the first flow channel. When the valve assembly rotates, the second exchange port can connect to the first flow channel.

7. The processing device of claim 6, wherein, The exchange channel also has a pressurization port, which is spaced apart from the first exchange port and the second exchange port. When the valve assembly rotates, the pressurization port can communicate with the first flow channel.

8. The processing device of claim 6, wherein, The shell assembly also has a second flow channel and a vent chamber spaced apart. The reaction mechanism also has a second reaction channel, which is connected to the reaction chamber and the second flow channel. The exchange valve also has a transfer channel that is independently set relative to the exchange channel. The transfer channel has a first transfer port and a second transfer port spaced apart. When the second exchange port is connected to the first flow channel, the first transfer port is connected to the vent chamber, and the second transfer port is connected to the second flow channel.

9. The processing device of claim 6, wherein, The reaction mechanism includes a reaction plate and a cover film. The reaction plate is connected to the shell assembly. The reaction cavity extends through the reaction plate along its thickness direction. There are two cover films, which are located on opposite sides of the reaction plate along its thickness direction and cover the reaction cavity. The first reaction channel is located inside the reaction plate.

10. The processing device of claim 1, wherein, The host also includes a liquid-proof and breathable membrane, which is disposed on the shell assembly and seals the openings of the buffer chamber, the collection chamber, and the accommodating chamber away from the exchange valve.

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