Enzyme solution filter sterilization device

Abstract

The utility model relates to experimental equipment technical field, concretely is a kind of enzyme solution filtration sterilization device, including temperature control box, placing shell, needle type filter, syringe, test tube, support frame and limiting component;Placing shell is slidably installed in the middle part of baffle inside temperature control box, the inside of placing shell is equipped with the needle type filter for carrying out filtration sterilization to enzyme solution in use state, syringe is installed at the top of needle type filter;Support frame is installed at the bottom end of placing shell.The utility model places placing shell in the inside of temperature control box by support frame, and test tube is vertically placed in support frame by supporting component, simultaneously, needle type filter is in placing shell, the outlet of needle type filter is inserted into test tube, and enzyme solution is directly conveyed by syringe, and then needle type filter filters and is conveyed to test tube, reduce the loss condition of larger that appears in enzyme solution filtration.

Description

Technical Field

[0001] This utility model relates to the field of experimental equipment technology, specifically to an enzyme solution filtration and sterilization device. Background Technology

[0002] Recombinant enzyme solution refers to an enzyme protein solution obtained through expression and purification using genetic engineering technology. In the process of preparing enzyme protein solution, in order to inhibit the thermal motion of enzyme molecules and reduce the risk of aggregation and denaturation, sterilization is carried out by filtration in a low-temperature environment, which is a method of sterilizing enzyme protein solution.

[0003] Chinese patent document CN208780536U discloses a sterile low-temperature filtration device, including a box with a top-opening door and a transparent front-opening door. The bottom wall of the box is a cooling base with a refrigeration system. A touch screen temperature adjustment display, a power switch, and an external power cord are provided on the side wall of the cooling base. Handles are provided on the outer side walls of the top-opening door and the transparent front-opening door near the opening side. The inner cavity of the box is divided into an upper chamber and a lower chamber by a horizontally arranged partition. A funnel hole for holding a filter funnel is opened on the partition. A first ultraviolet sterilization lamp is provided on the inner side wall of the upper chamber, and a second ultraviolet sterilization lamp is provided on the inner side wall of the lower chamber opposite to the first ultraviolet sterilization lamp.

[0004] In the above scheme, the collection container is placed in the lower chamber and the filter funnel is placed in the upper chamber with its lower end extending into the collection container. Suitable filter paper is placed in the filter funnel for filtration. When filtering small-volume enzyme protein solutions (such as solutions with a volume of less than 10 ml), the enzyme protein solution will adhere to the inner wall of the filter funnel, resulting in a large loss of sterilization in a single filtration of small-volume enzyme protein solutions, which affects the filtration effect. Utility Model Content

[0005] The purpose of this invention is to address the problems existing in the background technology by proposing an enzyme solution filtration and sterilization device.

[0006] The technical solution of this utility model is: an enzyme solution filtration and sterilization device, comprising a temperature control box, a placement shell, a needle filter, a syringe, a test tube, a support frame, and a limiting component;

[0007] The placement shell is slidably installed in the middle of the partition inside the temperature control box. The inside of the placement shell is equipped with a needle-type filter for filtering and sterilizing the enzyme solution during use. The syringe is installed at the top of the needle-type filter.

[0008] The support frame is installed at the bottom of the housing, and the inner side of the support frame is provided with a support component for vertically placing the test tube and connecting the test tube to the filter assembly in the use state;

[0009] The limiting component is located at the top of the housing and connected to the filter component.

[0010] Preferably, the support frame includes a base plate, a sliding column, and an internally threaded tube;

[0011] The internally threaded tube is located at the bottom of the housing;

[0012] The number of sliding pins is at least two, and the two sliding pins are symmetrically installed at the bottom end of the internally threaded pipe;

[0013] The base plate is installed at the bottom of the two housings and connected to the inner bottom of the temperature control box.

[0014] Preferably, the bottom end of the housing is provided with an external threaded tube, and when the housing and the internal threaded tube are installed together, the external threaded tube is accommodated in the internal threaded tube.

[0015] Preferably, the support assembly includes a tray and a first spring;

[0016] The tray is positioned above the base plate and fitted over the outside of the sliding column;

[0017] The first spring is sleeved on the outside of the slide column and located between the tray and the base plate.

[0018] Preferably, a limiting groove is provided at the top center of the tray, and the bottom end of the test tube is located inside the limiting groove.

[0019] Preferably, a collar is provided above the tray, which is fitted onto the slide column and wraps around the outside of the test tube, and a second spring is provided below the collar, which is fitted onto the outside of the slide column and movably connected to the tray.

[0020] Preferably, the limiting component consists of a spring and a support column;

[0021] The support column is installed at the top of the housing;

[0022] The spring is U-shaped and is mounted on the top of the support column and wrapped around the outside of the syringe.

[0023] Preferably, the opening of the spring is smaller than the size of the syringe, and the end of the spring is curved to the side.

[0024] Compared with the prior art, the above-mentioned technical solution of this utility model has the following beneficial technical effects:

[0025] This invention places the placement shell inside the temperature control chamber using a support frame, and vertically places the test tube in the support frame using a support assembly. At the same time, a needle filter is placed in the placement shell, with the outlet of the needle filter inserted into the test tube. The enzyme solution is then directly delivered to the test tube using a syringe, thereby allowing the needle filter to filter and deliver the solution to the test tube, reducing the significant loss that may occur during enzyme solution filtration. Attached Figure Description

[0026] Figure 1 This is a perspective view of one embodiment of the present invention.

[0027] Figure 2 This is a schematic diagram of the placement shell structure in one embodiment of the present invention.

[0028] Figure 3 This is a cross-sectional schematic diagram of the placement shell structure in one embodiment of the present invention.

[0029] Reference numerals: 1. Temperature control box; 2. Housing shell; 3. Needle filter; 4. Syringe; 5. Spring; 6. Support column; 7. Base plate; 8. Test tube; 9. Tray; 10. First spring; 11. Second spring; 12. Sliding column; 13. Internally threaded tube; 14. Collar; 15. Externally threaded tube; 16. Limiting groove. Detailed Implementation

[0030] Example 1

[0031] like Figure 1-3 As shown, the present invention proposes an enzyme solution filtration and sterilization device, which includes a temperature control box 1, a placement shell 2, a needle filter 3, a syringe 4, a test tube 8, a support frame, and a limiting component.

[0032] The placement shell 2 is slidably installed in the middle of the partition inside the temperature control box 1. The inside of the placement shell 2 is provided with a needle filter 3 for filtering and sterilizing the enzyme solution in use. The syringe 4 is installed at the top of the needle filter 3. In use, an electric push rod (not shown in the figure) can be set inside the temperature control box 1 so that the syringe 4 can deliver the enzyme solution to the needle filter 3 through the electric push rod.

[0033] The support frame is installed at the bottom of the housing 2. The inner side of the support frame is provided with a support component for vertically placing the test tube 8 and connecting the test tube 8 to the filter assembly in the use state.

[0034] The limiting component is located at the top of the placement shell 2 and connected to the filter component. It is used to limit the position of the syringe 4 during use. The limiting component and the placement shell 2 can be plugged in, so that the placement shell 2 and the limiting component can be detachably connected. Since the funnel is conical, the inner side of the placement shell 2 can be used to place a needle filter 3 or a filter funnel, thereby improving the applicability of the placement shell 2.

[0035] In this embodiment, temperature control is performed inside the temperature control chamber 1 to maintain a low-temperature environment (e.g., 2-8℃). The placement shell 2 is placed inside the temperature control chamber 1 via a support frame, and the test tube 8 is placed on the support assembly in the middle of the support frame, so that the support assembly vertically fixes the test tube 8. At the same time, the needle filter 3 and syringe 4 are placed inside the temperature control chamber 1 for pre-cooling. The syringe 4 draws the enzyme solution and is placed in the limiting assembly, connecting the syringe 4 to the needle filter 3. The syringe 4 delivers the enzyme solution to the needle filter 3, which then filters the enzyme solution and delivers it to the test tube 8, reducing the significant loss of enzyme solution during filtration.

[0036] Example 2

[0037] like Figure 2-3 As shown, the enzyme solution filtration and sterilization device proposed in this utility model differs from the first embodiment in that the support frame includes a base plate 7, a sliding column 12, and an internally threaded tube 13.

[0038] The internally threaded tube 13 is located at the bottom of the housing 2;

[0039] The number of sliding pins 12 is at least two, and the two sliding pins 12 are symmetrically installed at the bottom end of the internally threaded tube 13;

[0040] The base plate 7 is installed at the bottom of the two housings 2 and connected to the inner bottom of the temperature control box 1.

[0041] In an optional embodiment, the bottom end of the housing 2 is provided with an external threaded tube 15. When the housing 2 and the internal threaded tube 13 are installed together, the external threaded tube 15 is accommodated in the internal threaded tube 13. This is used to fix the housing 2 and the internal threaded tube 13 together during use, so that the housing 2 and the internal threaded tube 13 are connected as one unit. This makes the positioning connection between the needle filter 3 and the test tube 8 more accurate, and allows the internal threaded tube 13 to move with the housing 2 to the outside of the temperature control box 1, making it easy to disassemble and assemble.

[0042] In this embodiment, the internal threaded tube 13 is connected to the placement shell 2 through the external threaded tube 15, and the base plate 7 is connected to the inner bottom of the temperature control box 1. At the same time, the position of the internal threaded tube 13 is limited by the sliding column 12, thereby limiting the position of the placement shell 2, and making it more convenient to place the test tube 8 onto the support assembly.

[0043] Example 3

[0044] like Figure 2-3 As shown, the enzyme solution filtration and sterilization device proposed in this utility model differs from the first embodiment in that the support assembly includes a tray 9 and a first spring 10.

[0045] The tray 9 is positioned above the base plate 7 and fitted onto the outside of the slide column 12;

[0046] The first spring 10 is sleeved on the outside of the slide column 12 and located between the tray 9 and the base plate 7.

[0047] In an optional embodiment, a limiting groove 16 is provided at the top center of the tray 9, and the bottom end of the test tube 8 is located inside the limiting groove 16, which is used to limit the position of the bottom end of the test tube 8 during use and improve the stability of the test tube 8.

[0048] In an optional embodiment, a collar 14 is provided above the tray 9, which is sleeved on the slide column 12 and wraps around the outside of the test tube 8. A second spring 11 is provided below the collar 14, which is sleeved on the outside of the slide column 12 and movably connected to the tray 9. This spring is used to limit the position of the collar 14 during use, so that the collar 14 is sleeved on the outside of the test tube 8, so that the test tube 8 is placed vertically and the opening of the test tube 8 is directly opposite the outlet of the needle filter 3.

[0049] In this embodiment, by compressing the first spring 10 driven by the tray 9 and moving the collar 14 upward, the test tube 8 is placed in the limiting groove 16. The test tube 8 and the through hole in the middle of the collar 14 are placed coaxially. At the same time, the collar 14 slides downward on the sliding column 12 and is sleeved on the outside of the test tube 8 by gravity. The position of the collar 14 is limited by the second spring 11, so that the collar 14 is sleeved in the middle of the test tube 8. The first spring 10 rebounds, causing the first spring 10 to drive the base plate 7 upward. At the same time, the base plate 7 drives the collar 14 to move upward synchronously through the second spring 11, so that the top of the test tube 8 is located in the external threaded tube 15. This makes the placement of the test tube 8 more stable and the positioning more accurate.

[0050] Example 4

[0051] like Figure 1-2 As shown, the enzyme solution filtration and sterilization device proposed in this utility model differs from Embodiment 1 in that the limiting component spring piece 5 and the support column 6 are used.

[0052] The support column 6 is installed on the top of the housing 2;

[0053] The spring 5 is U-shaped and is mounted on the top of the support column 6 and wrapped around the outside of the syringe 4.

[0054] In an optional embodiment, the opening of the spring 5 is smaller than that of the syringe 4, and the end of the spring 5 is bent laterally to guide the syringe 4 into the spring 5 during use and to limit the position of the syringe 4 by the spring 5.

[0055] In this embodiment, when the syringe 4 moves towards the inside of the spring 5, the syringe 4 is limited and the spring 5 is supported by the support column 6, so that the spring 5 supports the syringe 4 and makes the syringe 4 more stable.

[0056] In this invention, the internally threaded tube 13 is connected to the housing 2 via the externally threaded tube 15, and the base plate 7 is connected to the inner bottom of the temperature control box 1. Simultaneously, the position of the internally threaded tube 13 is limited by the sliding column 12, thereby limiting the position of the housing 2. The tray 9 moves downward, compressing the first spring 10 and simultaneously moving the collar 14 upward, placing the test tube 8 in the limiting groove 16. The test tube 8 and the through hole in the middle of the collar 14 are placed coaxially. Gravity causes the collar 14 to slide downward on the sliding column 12 and be fitted onto the outside of the test tube 8. The second spring 11 limits the position of the collar 14, placing it at the middle of the test tube 8. The first spring 10 then rebounds, causing the base plate 7 to move upward. Simultaneously, the base plate 7, through the second spring 11, moves the collar 14 upward, placing the top of the test tube 8 at... In the external threaded tube 15, the test tube 8 is placed vertically. At the same time, the needle filter 3 and the syringe 4 are placed inside the housing 2 and connected to the test tube 8, and the inner side of the spring plate 5 is connected to the needle filter 3, respectively. The temperature control box 1 is kept in a low temperature environment (e.g., 2-8℃). The needle filter 3 and the syringe 4 are pre-cooled. At the same time, the syringe 4 draws the enzyme solution and is placed back in the spring plate 5 and connected to the needle filter 3. The temperature control box 1 is kept in a low temperature environment. The syringe 4 is operated by the operator or by an electric lifting rod installed inside the temperature control box 1 so that the syringe 4 delivers the enzyme solution directly to the needle filter 3. After filtration, the needle filter 3 delivers the solution directly to the test tube 8, reducing the large loss that occurs when filtering small volumes of enzyme solution.

[0057] The embodiments of the present invention have been described in detail above with reference to the accompanying drawings. However, the present invention is not limited thereto. Various changes can be made within the scope of knowledge possessed by those skilled in the art without departing from the spirit of the present invention.

Claims

1. An enzyme solution filtration and sterilization device, characterized in that, Includes a temperature control box (1), a housing (2), a needle filter (3), a syringe (4), test tubes (8), a support frame, and a limiting assembly; The placement shell (2) is slidably installed in the middle of the partition inside the temperature control box (1). The inner side of the placement shell (2) is provided with a needle filter (3) for filtering and sterilizing the enzyme solution in use. The syringe (4) is installed on the top of the needle filter (3). The support frame is installed at the bottom of the housing (2), and the inner side of the support frame is provided with a support component for vertically placing the test tube (8) and connecting the test tube (8) to the filter assembly in the use state; The limiting component is set at the top of the placement shell (2) and connected to the filter component.

2. The enzyme solution filtration and sterilization device according to claim 1, characterized in that, The support frame includes a base plate (7), a sliding column (12), and an internally threaded tube (13). The internally threaded tube (13) is located at the bottom of the housing (2); The number of sliding pins (12) is at least two, and the two sliding pins (12) are symmetrically installed at the bottom end of the internally threaded tube (13); The base plate (7) is installed at the bottom of the two placement shells (2) and connected to the inner bottom of the temperature control box (1).

3. The enzyme solution filtration and sterilization device according to claim 2, characterized in that, The bottom end of the housing (2) is provided with an external threaded tube (15). When the housing (2) and the internal threaded tube (13) are installed together, the external threaded tube (15) is accommodated in the internal threaded tube (13).

4. The enzyme solution filtration and sterilization device according to claim 3, characterized in that, The support assembly includes a tray (9) and a first spring (10); The tray (9) is placed above the base plate (7) and sleeved on the outside of the sliding column (12); The first spring (10) is sleeved on the outside of the slide (12) and located between the tray (9) and the base plate (7).

5. The enzyme solution filtration and sterilization device according to claim 4, characterized in that, A limiting groove (16) is provided at the top center of the tray (9), and the bottom end of the test tube (8) is located inside the limiting groove (16).

6. The enzyme solution filtration and sterilization device according to claim 4, characterized in that, A collar (14) is provided above the tray (9), which is fitted on the slide column (12) and wrapped around the outside of the test tube (8). A second spring (11) is provided below the collar (14), which is fitted on the outside of the slide column (12) and movably connected to the tray (9).

7. The enzyme solution filtration and sterilization device according to claim 1, characterized in that, The limiting component includes a spring (5) and a support column (6); The support column (6) is installed on the top of the housing (2); The spring (5) is U-shaped and is mounted on the top of the support column (6) and wrapped around the outside of the syringe (4).

8. The enzyme solution filtration and sterilization device according to claim 7, characterized in that, The opening of the spring (5) is smaller than that of the syringe (4), and the end of the spring (5) is bent to the side.

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