A device for storing fecal microorganisms at room temperature
By designing a room-temperature preservation device for fecal microorganisms and utilizing the innovative structure of the sampling component and preservation solution component, the problem of microbial exposure and inactivation caused by multiple transfers during fecal sampling was solved, thereby improving the reliability of sample preservation and the accuracy of data analysis.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- GUANGZHOU MAITUIN TECHNOLOGY CO LTD
- Filing Date
- 2025-08-11
- Publication Date
- 2026-07-07
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Figure CN224467787U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of biological testing technology, specifically to a device for preserving fecal microorganisms at room temperature. Background Technology
[0002] Fecal microbiota refers to the microbial community present in fecal samples, mainly including bacteria, fungi, viruses, and archaea. They are an important component of the human gut microbiota, reflecting the composition and functional state of the gut microbiota. Microorganisms in feces help the host digest food, break down dietary fiber, and produce short-chain fatty acids; stimulate the development of the intestinal mucosal immune system, regulate immune responses, and prevent pathogen invasion; form biofilms to prevent pathogen attachment and colonization, and maintain the integrity of the intestinal mucosa.
[0003] Analyzing the structure of fecal microbial communities can aid in the diagnosis of intestinal and metabolic diseases. Furthermore, microbial DNA extracted from feces can be used in various molecular biology experiments, providing reliable samples for biological research. After fecal sampling, without cryogenic freezing, the quantity, types, and abundance of microorganisms in the feces will continuously change over time, affecting subsequent extraction and data analysis. Existing technologies include preservation solutions for storing fecal samples at room temperature; however, in practice, fecal sampling, sealing, and subsequent addition of preservation solutions require multiple transfer operations. These transfers pose a risk of microbial exposure and inactivation, impacting data analysis and indicating room for improvement. Utility Model Content
[0004] This invention aims to solve the aforementioned technical problem that multiple transfer operations are required during the process of fecal sampling, sealing, and subsequent addition of preservation solution, and that microorganisms are at risk of exposure and inactivation during the transfer process. It provides a fecal microorganism room temperature preservation device.
[0005] To solve the above-mentioned technical problems, the technical solution provided by this utility model is: a fecal microorganism room temperature preservation device, including a sampling component and a preservation liquid component connected vertically;
[0006] The sampling assembly includes a transparent cylindrical shell with graduation lines on it; a sealing cap is threaded to the lower end of the shell; a sampling rod is installed inside the shell and moves up and down, with the lower end of the sampling rod extending out of the shell;
[0007] The preservation fluid assembly includes a bulb-shaped capsule, with the upper end of the sampling rod extending out of the capsule and having a limiting structure; a limiting ring is provided inside the capsule near its lower end, and a cylindrical liquid channel is provided between the bottom surface of the limiting ring and the lower end of the capsule, with the lower end of the liquid channel communicating with the shell; an elastic telescopic tube is provided at the lower end of the capsule and within the liquid channel, and the sampling rod is located inside the elastic telescopic tube; the elastic telescopic tube has a shoulder that can block the limiting ring.
[0008] Furthermore, a fixing ring is provided on the outer side of the lower end of the housing, and the outer side of the fixing ring is threaded, and the upper end of the sealing cover is provided with an internal threaded neck that can be threadedly connected to the fixing ring.
[0009] Furthermore, the sealing cover is frustum-shaped with anti-slip particles on the bottom surface; the bottom surface of the fixing ring is provided with an annular sealing gasket.
[0010] Furthermore, the limiting structure includes a corrugated tube at the upper end of the bladder, a top plate at the upper end of the corrugated tube, and the upper end of the sampling rod extending out of the corrugated tube and fixedly connected to the top plate.
[0011] Furthermore, the elastic telescopic tube includes a lower tube body and an upper tube body that is movably disposed on the outer side of the upper end of the lower tube body; the longitudinal section of the upper tube body is inverted T-shaped, and a spring is provided between its shoulder and the lower tube body.
[0012] Furthermore, the shoulder of the upper tube body slopes downward from the inside out; the top surface of the limiting ring slopes upward from the inside out.
[0013] Furthermore, the upper end of the pouch is provided with a hollow cylindrical insert that can accommodate the upper tube.
[0014] Furthermore, a handheld portion is provided on the outer side of the housing near the upper end.
[0015] The advantages of this utility model compared with the prior art are as follows:
[0016] The preservation solution for preserving feces at room temperature is stored in the upper part of the shell and sealed. After sampling and sealing the sampling rod, the preservation solution is directly introduced into the shell by squeezing the capsule to soak the fecal sample. During the process, the exposure of the fecal sample and the preservation solution is reduced, thus ensuring the survival time of the microorganisms. Attached Figure Description
[0017] Figure 1 This is a schematic diagram of the structure of this utility model.
[0018] Figure 2 This is a schematic diagram of the structure of this utility model. Figure 2 .
[0019] Figure 3 This is a cross-sectional schematic diagram of the present invention.
[0020] Figure 4 This is a schematic diagram of the cross-sectional structure of the elastic telescopic tube of this utility model.
[0021] Figure 5 This is a utility model Figure 3 Enlarged view of point A in the middle.
[0022] As shown in the figure: 1. Shell, 2. Scale line, 3. Sealing cover, 4. Fixing ring, 5. Anti-slip particles, 6. Sampling rod, 7. Bag, 8. Limiting ring, 9. Top plate, 10. Limiting ring, 11. Liquid channel, 12. Elastic telescopic tube, 13. Lower tube body, 14. Upper tube body, 15. Spring, 16. Insert, 17. Handheld part. Detailed Implementation
[0023] The present invention will now be described in further detail with reference to the accompanying drawings.
[0024] Example 1, in conjunction with Appendix Figure 1 , 2 A fecal microorganism room temperature preservation device, comprising a sampling component and a preservation liquid component connected vertically;
[0025] The sampling assembly includes a transparent cylindrical shell 1 with scale lines 2 on it, which facilitates observation of the amount of preservation liquid entering the shell 1.
[0026] The lower end of the housing 1 is threadedly connected to a sealing cover 3; specifically, a fixing ring 4 is provided on the outer side of the lower end of the housing 1, and the outer side of the fixing ring 4 is threaded, and the upper end of the sealing cover 3 is provided with an internal threaded neck that can be threadedly connected to the fixing ring 4; by threading the sealing cover 3 to the lower end of the housing 1, the housing 1 can be sealed.
[0027] Based on the above structure, the sealing cover 3 is frustum-shaped, and the bottom surface is provided with anti-slip particles 5. The frustum shape increases the bottom area of the sealing cover 3, which increases the contact area with the table surface when placed vertically, thereby improving stability. The bottom surface of the fixing ring 4 is provided with an annular sealing gasket, which improves the sealing between the two when it is threadedly connected to the sealing cover 3, thus preventing leakage of the storage liquid.
[0028] A sampling rod 6 is provided inside the housing 1, which can move up and down. The lower end of the sampling rod 6 can extend out of the housing 1 to facilitate sampling.
[0029] In addition, a handle 17 is provided on the outside of the shell 1 and near the top. Since the pouch 7 mentioned below is a soft shell, it may be accidentally squeezed when held, causing the preservation liquid to leak prematurely. Therefore, it can be taken out through the hard handle 17.
[0030] Combined with appendix Figure 1 , 23, 4, 5, The preservation fluid assembly includes a bulb-shaped capsule 7, with the upper end of the sampling rod 6 extending out of the capsule 7 and having a limiting structure; specifically, the limiting structure includes a corrugated tube 8 at the upper end of the capsule 7, a top plate 9 at the upper end of the corrugated tube 8, and the upper end of the sampling rod 6 extending out of the corrugated tube 8 and fixedly connected to the top plate 9; the corrugated tube 8 is a rigid plastic corrugated tube, which maintains its original shape when not under force and can be folded when under force. The sampling rod 6 is pushed out from the lower end of the housing 1 to take a sample. After the sample is taken, the top plate 9 is pulled outward to drive the corrugated tube 8 back to its original position, and the end of the sampling rod 6 returns to the housing 1. The sealing cover 3 is then installed.
[0031] Based on the above structure, a limiting ring 10 is provided inside the bag 7 near the lower end, and a cylindrical liquid channel 11 is provided between the bottom surface of the limiting ring 8 and the lower end of the bag 7. The lower end of the liquid channel 11 is connected to the shell 1; thus, the room temperature preservation liquid inside the bag 7 can enter the shell 1 through the liquid channel 11. It is worth mentioning that the fecal room temperature preservation liquid is existing technology and is used to prolong the survival time of microorganisms in feces under room temperature conditions.
[0032] Based on the above structure, an elastic telescopic tube 12 is provided at the lower end of the capsule 7 and inside the liquid channel 11, and the sampling rod 6 is located inside the elastic telescopic tube 12. Specifically, the elastic telescopic tube 12 is provided with a shoulder that can block the limiting ring 10. The elastic telescopic tube 12 includes a lower tube body 13 and an upper tube body 14 that is movable on the outer side of the upper end of the lower tube body 13. The longitudinal section of the upper tube body 14 is inverted T-shaped, and a spring 15 is connected between its shoulder and the lower tube body 13. Thus, in the above structure, when the capsule 7 is squeezed, the air and preservation liquid in the capsule 7 will simultaneously squeeze the upper tube body 14 downward, causing it to move downward, thereby exposing the liquid channel 11. The preservation liquid can enter the shell 1 to soak the fecal sample. When the pressure is stopped, the spring 15 loses its squeezing force and returns to its original position. The upper end of the upper tube body 14 can then block the limiting ring 10 again, achieving a relative seal of the preservation liquid. It is worth mentioning that the capsule 7 is made of rubber material with a thickness of 2-4mm, which can maintain its shape stability when not under force and can deform under force.
[0033] Based on the above structure, the shoulder of the upper tube 14 is inclined downward from the inside to the outside; the top surface of the limiting ring 10 is inclined upward from the inside to the outside, which facilitates the flow of the preservation liquid and makes it less likely to generate dead corners.
[0034] In addition, the upper end of the bag 7 is provided with a hollow cylindrical insert 16 that can accommodate the upper tube 14, which can restrict the movement path of the upper tube 14 and make it less likely to tilt; in order to prevent the preservation liquid from entering the upper tube 14, the upper end of the upper tube 14 can be sealed.
[0035] The present invention and its embodiments have been described above. This description is not restrictive, and the accompanying drawings are only one embodiment of the present invention; the actual structure is not limited thereto. In conclusion, if those skilled in the art are inspired by this description and design similar structures and embodiments without departing from the inventive spirit of the present invention, such designs should fall within the protection scope of the present invention.
Claims
1. A device for preserving fecal microorganisms at room temperature, characterized in that: Includes a sampling assembly and a preservation solution assembly connected vertically; The sampling assembly includes a transparent cylindrical shell (1) with scale lines (2) on it; a sealing cover (3) is threaded to the lower end of the shell (1); a sampling rod (6) is provided inside the shell (1) and moves up and down, with the lower end of the sampling rod (6) extending out of the shell (1); The preservation fluid assembly includes a bulb-shaped capsule (7), the upper end of the sampling rod (6) extends out of the capsule (7) and is provided with a limiting structure; a limiting ring (10) is provided inside the capsule (7) and near the lower end, a cylindrical liquid channel (11) is provided between the bottom surface of the limiting ring (10) and the lower end of the capsule (7), the lower end of the liquid channel (11) is connected to the shell (1); an elastic telescopic tube (12) is provided at the lower end of the capsule (7) and inside the liquid channel (11), the sampling rod (6) is located inside the elastic telescopic tube (12); the elastic telescopic tube (12) is provided with a shoulder that can block the limiting ring (10).
2. The fecal microorganism room temperature preservation device according to claim 1, characterized in that: The lower outer side of the housing (1) is provided with a fixing ring (4), the outer side of the fixing ring (4) is provided with a thread, and the upper end of the sealing cover (3) is provided with an internal thread neck that can be threadedly connected to the fixing ring (4).
3. The fecal microorganism room temperature preservation device according to claim 2, characterized in that: The sealing cover (3) is frustum-shaped and has anti-slip particles (5) on the bottom surface; the fixing ring (4) has an annular sealing gasket on the bottom surface.
4. The fecal microbial preservation device at room temperature according to claim 1, characterized in that: The limiting structure includes a corrugated tube (8) at the upper end of the bag (7), a top plate (9) at the upper end of the corrugated tube (8), and the upper end of the sampling rod (6) extends out of the corrugated tube (8) and is fixedly connected to the top plate (9).
5. The fecal microorganism room temperature preservation device according to claim 1, characterized in that: The elastic telescopic tube (12) includes a lower tube body (13) and an upper tube body (14) that is movably disposed on the outer side of the upper end of the lower tube body (13); the longitudinal section of the upper tube body (14) is inverted T-shaped, and a spring (15) is provided between its shoulder and the lower tube body (13).
6. The fecal microorganism room temperature preservation device according to claim 5, characterized in that: The shoulder of the upper tube (14) slopes downward from the inside to the outside; the top surface of the limiting ring (10) slopes upward from the inside to the outside.
7. The fecal microorganism room temperature preservation device according to claim 6, characterized in that: The upper end of the bag (7) is provided with a hollow cylindrical insert (16) that can accommodate the upper tube (14).
8. The fecal microorganism room temperature preservation device according to claim 1, characterized in that: A hand-held part (17) is provided on the outside of the housing (1) and near the upper end.