A production system for improving the content of low-grafted glucose-based steviol glycosides

Abstract

The utility model discloses a production system of improving low grafting glucose base steviol glycoside content, including intercommunication enzymolysis reaction unit, high temperature cleavage unit, first separation unit, resin purification unit, first drying unit, crystallization unit, second separation unit, second drying unit, storage unit, enzymolysis reaction unit includes enzyme reaction jar, with the glycoside acceptor storage tank of enzyme reaction jar intercommunication, glycoside donor storage tank and enzyme preparation storage tank, high temperature cleavage unit includes, cleavage jar, resin purification unit includes first resin column, second resin column that intercommunication in proper order, and second resin column is connected with the analysis agent storage tank, crystallization unit includes the dissolving jar that communicates with first drying unit and the crystallization jar that communicates with the dissolving jar, and the dissolving jar is connected with the crystallization solvent storage tank. This production system passes through enzymolysis, high temperature cleavage conversion, two -stage resin selective enrichment and recrystallization refining, and the purity and yield of low grafting glucose base steviol glycoside in product have been improved significantly.

Description

Technical Field

[0001] This utility model relates to the field of natural extract technology, specifically to a production system for increasing the content of low-grafted glucosyl steviol glycosides. Background Technology

[0002] Steviol glycosides are natural high-intensity sweeteners extracted from stevia leaves. Due to their high sweetness, low calories, and good safety profile, they are widely used in the food, beverage, and pharmaceutical industries. Steviol glycosides contain various glycoside components, among which low-grafted glucosyl steviol glycosides have higher market value and application prospects because their taste is closer to sucrose, their sweetness is pure, and their aftertaste is low.

[0003] Currently, the main method for increasing the content of low-grafted glucosyl steviol glycosides in steviol glycoside products is through enzymatic modification. However, existing production processes have the following problems: due to the complexity of the reaction products, the target product typically contains low-grafted glucosyl steviol glycosides, high-grafted glucosyl steviol glycosides, unreacted substrates, pigments, impurities, etc. Conventional single separation or purification methods are insufficient to efficiently and selectively separate the target components, resulting in limited improvements in product purity and target component yield. Utility Model Content

[0004] The technical problem to be solved by this utility model is to provide a production system for improving the content of low-grafted glucosyl steviol glycosides in the product, which addresses the shortcomings of the existing technology. This production system significantly improves the purity and yield of low-grafted glucosyl steviol glycosides in the product through enzymatic hydrolysis, high-temperature pyrolysis and conversion, selective enrichment with two-stage resin and recrystallization purification.

[0005] To solve the above-mentioned technical problems, the technical solution of this utility model is as follows:

[0006] A production system for increasing the content of low-grafted glucosyl steviol glycosides includes an enzymatic hydrolysis reaction unit, a high-temperature pyrolysis unit, a first separation unit, a resin purification unit, a first drying unit, a crystallization unit, a second separation unit, a second drying unit, and a storage unit connected in series.

[0007] The enzymatic hydrolysis unit includes an enzyme reaction vessel and glycoside acceptor storage tank, glycoside donor storage tank, and enzyme preparation storage tank connected to the enzyme reaction vessel; the high-temperature pyrolysis unit includes a pyrolysis vessel connected to the enzyme reaction vessel; the resin purification unit includes a first resin column and a second resin column sequentially connected to the filtrate outlet of the first separation unit, the second resin column being connected to a desorption agent storage tank; the crystallization unit includes a dissolving tank connected to the first drying unit and a crystallization tank connected to the dissolving tank, the dissolving tank being connected to a crystallization solvent storage tank; and the storage unit includes a target product storage tank connected to the material outlet of the second drying unit.

[0008] Preferably, the first resin column contains an activated carbon adsorption layer, and the second resin column contains a macroporous adsorption resin layer.

[0009] Preferably, a concentration tank is also provided on the connecting pipe between the outlet of the second resin column and the first drying unit.

[0010] Preferably, the first separation unit includes a first plate and frame filter connected to the pyrolysis tank; the second separation unit includes a second plate and frame filter connected to the crystallization tank.

[0011] Preferably, the first drying unit includes a spray dryer; the second drying unit includes a drying tank connected to the solid outlet of the second plate and frame filter.

[0012] Preferably, the enzyme reaction vessel, drying vessel, and dissolving vessel are each equipped with a first stirring device, which includes a first stirring motor, a first stirring shaft connected to the first stirring motor, and a first stirring blade disposed on the first stirring shaft.

[0013] Preferably, the inner wall of the drying tank is provided with multiple non-parallel lifting plates.

[0014] Preferably, the crystallization tank is provided with a second stirring device, which includes a second stirring motor, a second stirring shaft connected to the second stirring motor, and a second stirring blade provided on the second stirring shaft.

[0015] Preferably, the second stirring shaft is also connected to a scraper that abuts against the inner wall of the crystallization tank via a connecting rod.

[0016] Due to the adoption of the above technical solution, the beneficial effects of this utility model are:

[0017] This invention provides a production system for increasing the content of low-grafted glucosyl steviol glycosides, comprising a connected enzymatic hydrolysis unit, a high-temperature pyrolysis unit, a first separation unit, a resin purification unit, a first drying unit, a crystallization unit, a second separation unit, a second drying unit, and a storage unit. This production system significantly and effectively increases the content of low-grafted glucosyl steviol glycosides in the final product through enzymatic hydrolysis optimization, high-temperature pyrolysis to remove byproducts, selective enrichment with two-stage resin, and recrystallization purification. The enzymatic hydrolysis unit of this production system includes an enzyme reaction vessel and glycoside acceptor storage tanks, glycoside donor storage tanks, and enzyme preparation storage tanks connected to the enzyme reaction vessel. By storing the glycoside acceptor, glycoside donor, and enzyme preparation in separate tanks, it is convenient to effectively control the feeding ratio, feeding sequence, and feeding speed, optimize the enzyme catalytic reaction conditions, and maximize the generation of the target low-grafted product. Furthermore, this production system further improves the yield of the target product by high-temperature pyrolysis of the feed solution after the enzyme catalytic reaction in a pyrolysis tank.

[0018] The resin purification unit of this production system includes a first resin column and a second resin column connected in sequence to the first separation unit. The second resin column is connected to a desorption agent storage tank. The first resin column contains an activated carbon adsorption layer, and the second resin column contains a macroporous adsorption resin layer. The first resin column removes pigments, small molecule organic impurities, and odor substances from the lysis solution. The second resin column selectively adsorbs the target product, thereby effectively separating the low-grafted target product from other steviol glycosides and residual impurities.

[0019] The enzyme reaction tank, drying tank, and dissolving tank of this production system are all equipped with a first stirring device, and the crystallization tank is equipped with a second stirring device. The above settings effectively improve production efficiency.

[0020] The drying tank of this production system is equipped with multiple non-parallel lifting plates on its inner wall, which greatly enhances the drying efficiency. The second stirring shaft of this production system is also connected to a scraper that abuts against the inner wall of the crystallization tank via a connecting rod. This prevents excessive accumulation and scaling of crystals on the inner wall and surface of the crystallization tank, ensuring effective heat transfer and crystallization surface area, and improving crystallization efficiency, product yield, and quality stability. Attached Figure Description

[0021] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0022] Figure 1 This is a schematic diagram of the structure of Embodiment 1 of this utility model;

[0023] In the diagram, 1. Enzyme reaction vessel; 2. Glycoside acceptor storage vessel; 3. Glycoside donor storage vessel; 4. Enzyme preparation storage vessel; 5. Pyrolysis vessel; 6. First plate and frame filter press; 7. First resin column; 8. Second resin column; 9. Activated carbon adsorption layer; 10. Macroporous adsorption resin layer; 11. Desorption agent storage vessel; 12. Concentration vessel; 13. Spray dryer; 14. Dissolving vessel; 15. Crystallization vessel; 16. Crystallization solvent storage vessel; 17. Second plate and frame filter press; 18. Drying vessel; 19. Target product storage vessel; 20. First stirring motor; 21. First stirring shaft; 22. First stirring blade; 23. Scraper plate; 24. Second stirring motor; 25. Second stirring shaft; 26. Second stirring blade; 27. Connecting rod; 28. Scraper. Detailed Implementation

[0024] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0025] Example 1

[0026] like Figure 1 As shown, a production system for increasing the content of low-grafted glucosyl steviol glycosides includes an enzymatic hydrolysis reaction unit, a high-temperature pyrolysis unit, a first separation unit, a resin purification unit, a first drying unit, a crystallization unit, a second separation unit, a second drying unit, and a storage unit connected in series.

[0027] The enzymatic hydrolysis reaction unit includes an enzyme reaction vessel 1 and a glycoside acceptor storage vessel 2, a glycoside donor storage vessel 3, and an enzyme preparation storage vessel 4 connected to the enzyme reaction vessel 1.

[0028] The high-temperature pyrolysis unit includes a pyrolysis tank 5 connected to the enzyme reaction tank 1. After enzymatic modification, this production system uses high-temperature pyrolysis to decompose byproducts and reduce the subsequent purification load. On the other hand, it may induce intramolecular rearrangement of steviol glycosides, promote the generation of low-grafted glucosyl steviol glycosides, and increase the content and yield of low-grafted glucosyl steviol glycosides in the target product.

[0029] The first separation unit includes a first plate and frame filter 6 connected to the pyrolysis tank 5;

[0030] The resin purification unit includes a first resin column 7 and a second resin column 8, which are sequentially connected to the filtrate outlet of the first separation unit. The first resin column 7 contains an activated carbon adsorption layer 9, and the second resin column 8 contains a macroporous adsorption resin layer 10. The second resin column 8 is connected to a desorbent storage tank 11. A concentration tank 12 is also provided on the connection pipe between the desorbate outlet of the second resin column and the first drying unit. The first resin column 7 uses activated carbon to adsorb small molecule impurities such as pigments and polyphenols, solving the problem of residual bitter substances in the mother liquor sugar. The second resin column 8 uses macroporous adsorption resin to selectively adsorb the target product, and combined with the desorbent, achieves the separation of high-purity, low-grafted glucosyl steviol glycosides.

[0031] The first drying unit includes a spray dryer 13;

[0032] The crystallization unit includes a dissolving tank 14 connected to the first drying unit and a crystallization tank 15 connected to the dissolving tank 14. The dissolving tank 14 is connected to a crystallization solvent storage tank 16.

[0033] The second separation unit includes a second plate and frame filter 17 connected to the crystallizer 15;

[0034] The second drying unit includes a drying tank 18 connected to the solid outlet of the second plate and frame filter 17;

[0035] The storage unit includes a target product storage tank 19 that is connected to the material outlet of the second drying unit.

[0036] Furthermore, in this embodiment, the enzyme reaction vessel 1, the drying vessel 18, and the dissolving vessel 14 are all equipped with a first stirring device. The first stirring device includes a first stirring motor 20, a first stirring shaft 21 connected to the first stirring motor 20, and a first stirring blade 22 provided on the first stirring shaft 21.

[0037] Furthermore, in this embodiment, the inner wall of the drying tank 18 is provided with multiple non-parallel lifting plates 23. The non-parallel lifting plates 23 can extend the material residence time and improve drying efficiency.

[0038] Furthermore, in this embodiment, the crystallization tank 15 is provided with a second stirring device, which includes a second stirring motor 24, a second stirring shaft 25 connected to the second stirring motor 24, and a second stirring blade 26 provided on the second stirring shaft 25.

[0039] Furthermore, in this embodiment, a scraper 28 that abuts against the inner wall of the crystallization tank 15 is also connected to the second stirring shaft 25 via a connecting rod 27. The combined arrangement of the scraper 28 and the second stirring device prevents crystal agglomeration and improves mass transfer efficiency, thereby improving crystallization efficiency.

[0040] The above description of the disclosed embodiments enables those skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention is not to be limited to the embodiments shown herein, but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A production system for increasing the content of low-grafted glucosyl steviol glycosides, characterized in that: It includes interconnected enzymatic hydrolysis reaction unit, high-temperature pyrolysis unit, first separation unit, resin purification unit, first drying unit, crystallization unit, second separation unit, second drying unit, and storage unit; The enzymatic hydrolysis unit includes an enzyme reaction vessel and glycoside acceptor storage tank, glycoside donor storage tank, and enzyme preparation storage tank connected to the enzyme reaction vessel; the high-temperature pyrolysis unit includes a pyrolysis vessel connected to the enzyme reaction vessel; the resin purification unit includes a first resin column and a second resin column sequentially connected to the filtrate outlet of the first separation unit, the second resin column being connected to a desorption agent storage tank; the crystallization unit includes a dissolving tank connected to the first drying unit and a crystallization tank connected to the dissolving tank, the dissolving tank being connected to a crystallization solvent storage tank; the storage unit includes a target product storage tank connected to the material outlet of the second drying unit.

2. The production system for increasing the content of low-grafted glucosyl steviol glycosides according to claim 1, characterized in that: The first resin column contains an activated carbon adsorption layer, and the second resin column contains a macroporous adsorption resin layer.

3. The production system for increasing the content of low-grafted glucosyl steviol glycosides according to claim 1, characterized in that: A concentration tank is also provided on the connecting pipe between the outlet of the second resin column and the first drying unit.

4. The production system for increasing the content of low-grafted glucosyl steviol glycosides according to claim 1, characterized in that: The first separation unit includes a first plate and frame filter that is connected to the pyrolysis tank; the second separation unit includes a second plate and frame filter that is connected to the crystallization tank.

5. The production system for increasing the content of low-grafted glucosyl steviol glycosides according to claim 1, characterized in that: The first drying unit includes a spray dryer; the second drying unit includes a drying tank connected to the solid outlet of the second plate and frame filter.

6. The production system for increasing the content of low-grafted glucosyl steviol glycosides according to claim 5, characterized in that: The enzyme reaction vessel, drying vessel, and dissolving vessel are each equipped with a first stirring device. The first stirring device includes a first stirring motor, a first stirring shaft connected to the first stirring motor, and a first stirring blade mounted on the first stirring shaft.

7. The production system for increasing the content of low-grafted glucosyl steviol glycosides according to claim 5, characterized in that: The inner wall of the drying tank is provided with multiple non-parallel lifting plates.

8. The production system for increasing the content of low-grafted glucosyl steviol glycosides according to claim 1, characterized in that: The crystallization tank is equipped with a second stirring device, which includes a second stirring motor, a second stirring shaft connected to the second stirring motor, and a second stirring blade disposed on the second stirring shaft.

9. A production system for increasing the content of low-grafted glucosyl steviol glycosides according to claim 8, characterized in that: The second stirring shaft is also connected to a scraper that abuts against the inner wall of the crystallization tank via a connecting rod.

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