Mass spectrometry sample protein pretreatment device

Abstract

The utility model relates to mass spectrum sample protein pretreatment technical field, concretely refers to a kind of mass spectrum sample protein pretreatment device, including detachable sleeve head structure, detachable sleeve head structure one side is equipped with cover through hinged structure;Detachable sleeve head structure includes sleeve body, and the middle part of sleeve body is horizontally equipped with filter plate one and filter plate two;Test tube is equipped below detachable sleeve head, and two clamping grooves are set in the inside of sleeve body, and sealing ring is clamped in clamping groove, and detachable sleeve head is clamped at test tube mouth place.The utility model solves the problem that the filter structure of existing pretreatment device is integrated with tube body, although simple structure, filter membrane is detachable, but sample is not easy to transfer to test tube after filtration, inconvenient operation, and poor applicability of microbiological experiment.

Description

Technical Field

[0001] This utility model relates to the field of mass spectrometry sample protein pretreatment technology, specifically to a mass spectrometry sample protein pretreatment device. Background Technology

[0002] Mass spectrometry for microbial identification is a rapid and accurate technique for classifying and identifying microorganisms. It is mainly performed by analyzing the mass spectra of specific proteins or peptides within microbial cells. The core of this technique is based on matrix-assisted laser desorption / ionization time-of-flight mass spectrometry (MALDI-TOF MS).

[0003] Chinese utility model patent application number CN202420922938.8 discloses a protein pretreatment device for mass spectrometry microbial identification samples. The device comprises a tube body, which is a non-perforated container; a cap detachably installed at the opening of the tube body; and a filter assembly detachably disposed within the tube body. The filter assembly includes a filter funnel, a first filter section, a connecting tube, and a second filter section. The filter funnel is sequentially connected to the first filter section, the connecting tube, and the second filter section. The filter assembly can be completed simply by installing the first and second filter membranes, making the operation simple, time-saving, and labor-saving. Furthermore, after cleaning, the first and second filter membranes can be replaced and reused, thereby reducing detection costs.

[0004] Although the device has a simple structure and the filter membrane is detachable, the overall filtration structure and the tube body are ultimately integrated. Since test tubes are mostly used to hold samples in microbiology experiments, this device is not convenient for transferring filtered samples into test tubes, making it unsuitable for microbiology experiments. Utility Model Content

[0005] The technical problem this invention aims to solve is that existing pretreatment devices integrate the filtration structure with the tube body. Although the structure is simple and the filter membrane is detachable, the filtered sample is not easily transferred to the test tube, making operation inconvenient and resulting in poor applicability to microbial experiments.

[0006] To solve the above problems, the technical solution adopted by this utility model is a mass spectrometry sample protein pretreatment device, including a detachable sleeve structure, and a cover is provided on one side of the detachable sleeve structure through a hinge structure; the detachable sleeve structure includes a sleeve body, and a filter plate one and a filter plate two are arranged laterally in the middle of the sleeve body; a test tube is provided below the detachable sleeve head, and two snap-fit ​​grooves are opened on the inner side of the sleeve body, and a sealing ring is snapped in the snap-fit ​​grooves, and the detachable sleeve head is snapped into the mouth of the test tube.

[0007] As a further embodiment of this utility model: an inverted cone is fixed in the middle of the sleeve to facilitate sample sliding; a slot is provided at the bottom inner side of the inverted cone, and a filter plate is inserted into the slot for secondary filtration.

[0008] As a further embodiment of this utility model: a placement groove is provided on the inner side of the inverted cone near the top, and a filter plate two is placed in the placement groove, which can perform preliminary filtration and is easy to disassemble.

[0009] As a further embodiment of this utility model: a convex ring is fixed on the upper side of the filter plate to facilitate the removal of the filter plate.

[0010] As a further embodiment of this utility model: the hinge structure includes hinge seats fixed on one side of the sleeve and the cover respectively, and a connecting plate is hinged between the two hinge seats. The hinge hole on the upper side of the connecting plate is a long slot hole, which facilitates the flexible opening and closing of the cover and improves the service life of the detachable sleeve.

[0011] As a further embodiment of this utility model: both the sleeve and the cover are fixed with protrusions on one side, and the two protrusions are connected and fixed by fastening bolts. By tightening the fastening bolts, the sleeve and the cover can be tightly sealed.

[0012] As a further embodiment of this invention, a sealing gasket is provided between the sleeve and the cover to improve the sealing effect.

[0013] Compared with the prior art, the advantages of this utility model are as follows: This application adds a detachable sleeve structure, which can be used for test tubes or measuring cups of appropriate diameter and other experimental equipment. It also has a double-layer filter structure to facilitate protein pretreatment and improve experimental efficiency. In addition, it adds a sealing ring and a sealing gasket to enhance the sealing effect and improve storage quality. Attached Figure Description

[0014] The accompanying drawings are provided to further illustrate the present invention and form part of the specification. They are used together with the embodiments of the present invention to explain the present invention, but do not constitute a limitation thereof. In the drawings:

[0015] Figure 1 This is a schematic diagram of the overall structure of a mass spectrometry sample protein pretreatment device according to the present invention.

[0016] Figure 2 This is a partial structural cross-sectional view of a mass spectrometry sample protein pretreatment device according to the present invention.

[0017] Figure 3 This is a schematic diagram of the detachable cap structure in a mass spectrometry sample protein pretreatment device of this utility model.

[0018] Figure 4 This is an enlarged view of part A of the mass spectrometry sample protein pretreatment device of this utility model.

[0019] In the attached image:

[0020] 1. Cover; 2. Sleeve; 3. Filter plate one; 4. Filter plate two; 5. Test tube; 6. Sealing ring; 7. Inverted cone; 8. Hinge seat; 9. Connecting plate; 10. Protrusion; 11. Sealing gasket; 12. Fastening bolt; 3.1. Protruding ring; 7.1. Slot; 7.2. Placement slot. Detailed Implementation

[0021] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0022] This utility model provides a technical solution to address the existing problems mentioned in the background art.

[0023] Combined with appendix Figure 1-4 It can be seen that the detachable sleeve structure includes a cover 1 connected by a hinge on one side; the detachable sleeve structure includes a sleeve 2, in which filter plate 3 and filter plate 4 are horizontally arranged in the middle; an inverted cone 7 is fixed in the middle of the sleeve 2 to facilitate sample sliding; a slot 7.1 is opened at the bottom of the inner side of the inverted cone 7, in which filter plate 3 is locked for secondary filtration; a protruding ring 3.1 is fixed on the upper side of filter plate 3 to facilitate removal of filter plate 3; a placement groove 7.2 is opened near the top of the inner side of the inverted cone 7, in which filter plate 4 is placed to perform preliminary filtration and facilitate disassembly;

[0024] The detachable sleeve has a test tube 5 below it. The inner side of the sleeve 2 has two snap-fit ​​grooves, and a sealing ring 6 is snapped into the snap-fit ​​groove. The detachable sleeve is snapped into the mouth of the test tube 5. The hinge structure includes hinge seats 8 fixed on one side of the sleeve 2 and the cover 1 respectively. A connecting plate 9 is hinged between the two hinge seats 8. The hinge hole on the upper side of the connecting plate 9 is a long slot hole, which facilitates the flexible opening and closing of the cover 1 and improves the service life of the detachable sleeve. A protrusion 10 is fixed on one side of both the sleeve 2 and the cover 1. The two protrusions 10 are connected and fixed by a fastening bolt 12. By tightening the fastening bolt 12, the sleeve 2 and the cover 1 can be tightly sealed. A sealing gasket 11 is sandwiched between the sleeve 2 and the cover 1 to improve the sealing effect.

[0025] The working principle of this application is as follows: When performing protein pretreatment, firstly, two sealing rings 6 are installed in the snap-fit ​​groove on the inner side of the sleeve 2. Then, the sleeve 2 can be snapped into the mouth of the test tube 5. Next, filter plate 3 is snapped into the slot 7.1, and filter plate 4 is placed in the placement slot 7.2. Then, the sample can be transferred to filter through the double-layer filtration structure. After filtration, filter plate 3 and filter plate 4 can be removed and cleaned. When sample storage is required, the cover 1 can be closed, and then the fastening bolts 12 are tightened into the connecting plate 10 to seal the sample for easy storage.

[0026] The present invention and its embodiments have been described above. This description is not restrictive, and the accompanying drawings are only one embodiment of the present invention; the actual structure is not limited thereto. In conclusion, if those skilled in the art are inspired by this description and design similar structures and embodiments without departing from the inventive spirit of the present invention, such designs should fall within the protection scope of the present invention.

Claims

1. A mass spectrometry sample protein pretreatment device, characterized in that: The detachable sleeve structure includes a cover (1) on one side of the detachable sleeve structure via a hinge structure; the detachable sleeve structure includes a sleeve (2), and a filter plate 1 (3) and a filter plate 2 (4) are provided horizontally in the middle of the sleeve (2); a test tube (5) is provided below the detachable sleeve, and two snap-fit ​​grooves are provided on the inner side of the sleeve (2), and a sealing ring (6) is snapped in the snap-fit ​​grooves. The detachable sleeve is snapped into the mouth of the test tube (5).

2. The mass spectrometry sample protein pretreatment device according to claim 1, characterized in that: The sleeve (2) has an inverted cone (7) fixed in the middle. The bottom of the inner side of the inverted cone (7) has a slot (7.1) and the filter plate (3) is locked in the slot (7.1).

3. The mass spectrometry sample protein pretreatment device according to claim 2, characterized in that: The inverted cone (7) has a placement groove (7.2) on its inner side near the top, and a filter plate (4) is placed in the placement groove (7.2).

4. The mass spectrometry sample protein pretreatment device according to claim 1, characterized in that: A protruding ring (3.1) is fixed on the upper side of the filter plate (3).

5. The mass spectrometry sample protein pretreatment device according to claim 1, characterized in that: The hinge structure includes hinge seats (8) fixed on one side of the sleeve (2) and the cover (1) respectively. A connecting plate (9) is hinged between the two hinge seats (8). The hinge hole on the upper side of the connecting plate (9) is a long slot hole.

6. The mass spectrometry sample protein pretreatment device according to claim 1, characterized in that: Both the sleeve (2) and the cover (1) are fixed with protrusions (10) on one side, and the two protrusions (10) are connected and fixed by fastening bolts (12).

7. The mass spectrometry sample protein pretreatment device according to claim 1, characterized in that: A sealing gasket (11) is sandwiched between the sleeve (2) and the cover (1).

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