A mass spectrometry kit

Abstract

The application provides a mass spectrum analysis kit to at least solve the technical problem that transportation affects analysis results in the prior art, comprising an inner shell and an outer shell, the inner shell is provided with a first closed inner cavity, the outer shell is provided with a second closed inner cavity, the inner shell is located in the second closed inner cavity, and a suspension is arranged in the second closed inner cavity so that the inner shell is in a suspended state in the second closed inner cavity. The suspension can make the inner shell in a suspended state, so that the inner shell is not directly rigidly connected with the outer shell when the outer shell is subjected to a severe impact, and therefore the inner shell can filter part of the impact or make the impact moderate under the action of the suspension. In addition, the suspension has a heat preservation function, so that the inner shell can be maintained at about 4 DEG C for a longer time, the mass spectrum analysis of proteins, polypeptides or other metabolites in the inner shell is more accurate, degradation of the proteins, polypeptides or other metabolites is avoided, and the analysis is not affected. In addition, the reagent subjected to homogenization treatment is prevented from being impacted, and stratification or precipitation of the reagent is avoided.

Description

Technical Field

[0001] This application relates to the field of transport containers, and more particularly to a mass spectrometry analysis kit. Background Technology

[0002] Mass spectrometry (MS) is a method that separates and detects charged ions according to their mass-to-charge ratio using electric and magnetic fields. It is used to determine the molecular weight, elemental composition, and structural information of compounds. MS equipment is relatively expensive, so many organizations need to send reagent kits containing reagents to specialized testing institutions for MS analysis. When performing MS analysis on solids such as proteins, a homogenizer is required to homogenize the sample, which then needs to be stored at approximately 4°C. During transfer, significant impacts should be avoided to prevent degradation of reagent homogeneity. While existing logistics systems are relatively advanced, the quality of delivery personnel varies, and the vehicles used also differ. Severe shaking during delivery may affect the analytical results.

[0003] Chinese patent application CN218464244U, entitled "A Reagent Kit for Mass Spectrometry Analysis," discloses a reagent kit for mass spectrometry analysis, comprising a kit, a cover plate, and a base plate. The kit has an internal groove, within which a buffer airbag is fixedly installed. An air vent is located at the top of the buffer airbag. A reinforcing layer is fixedly connected to the inner wall of the kit, and an insulation layer is fixedly connected to the inner wall of the reinforcing layer. A slot is located on the front of the upper surface of the kit. This reagent kit for mass spectrometry analysis, by installing a buffer airbag in the internal groove and introducing air into the buffer airbag through the air vent, reduces the impact of external shocks and vibrations on the reagent tubes inside the kit during movement. Furthermore, the insulation layer made of phenolic resin inside the kit maintains a relatively constant internal temperature, meeting the kit's requirements for preserving test tubes used in mass spectrometry analysis. However, the damping force of the airbag is relatively small. Although it can provide a buffering effect, it will still shake. Although phenolic resin has certain heat preservation properties, without other measures, the temperature will rise to a high level in the hot summer after a certain period of time, causing some substances in the reagent to degrade or precipitate, affecting the analysis results. Utility Model Content

[0004] This application provides a mass spectrometry analysis kit to at least address the technical problem of the impact of transportation on analytical results in the prior art.

[0005] According to this application, a mass spectrometry analysis kit is provided, including an inner shell and an outer shell. The inner shell has a first closed inner cavity, and the outer shell has a second closed inner cavity. The inner shell is located in the second closed inner cavity, and a suspension is provided in the second closed inner cavity so that the inner shell is in a suspended state in the second closed inner cavity.

[0006] Compared with existing technologies, the mass spectrometry analysis kit of this application has the following advantages:

[0007] This kit can be used for mass spectrometry analysis of proteins, peptides, and metabolites. The suspension keeps the inner shell suspended, so when the outer shell is subjected to severe impact, there is no direct rigid connection between the inner and outer shells. Therefore, under the action of the suspension, the inner shell can filter out part of the impact or mitigate it. In addition, the suspension has a heat-preserving function, allowing the inner shell to be maintained at around 4°C for a longer period of time, ensuring more accurate mass spectrometry analysis of proteins, peptides, or other metabolites inside and avoiding the impact of degradation on the analysis. Furthermore, the homogenized reagents should be protected from impact. Using the kit of this application can maintain the consistency of the reagents within a certain period of time, avoiding phenomena such as reagent stratification or precipitation.

[0008] In one embodiment, an elastic fixing band is provided in the second closed inner cavity of the outer shell. The overall density of the inner shell is greater than that of the suspension, and it is suspended in the second closed inner cavity through the elastic fixing band. The higher density of the inner shell allows it to have a smaller acceleration when the outer shell accelerates, that is, it can be subjected to a smaller impact. For example, when the reagent kit falls from a height, when the average density of the inner shell is twice that of the suspension, the outer shell is subjected to a force of twice the gravitational acceleration, and the suspension is also subjected to a force of nearly twice the gravitational acceleration, but the inner shell is only subjected to a force of one gravitational acceleration. Moreover, under the damping effect of the suspension, the inner shell will not shake repeatedly. In addition, the suspension can buffer temperature changes, that is, the temperature rise requires more energy to absorb, so it can be kept at a lower temperature to avoid changes in reagent composition that affect mass spectrometry analysis.

[0009] In one embodiment, the outer side of the inner shell is provided with a fixing lug, and the inner side of the outer shell is provided with a fixing ring. The elastic fixing band passes around the fixing lug and the fixing ring in sequence, so that the inner shell remains suspended in the middle position under the combined action of the elastic fixing band and the suspension.

[0010] In one embodiment, the first enclosed inner cavity of the inner shell is provided with a tube rack, which can hold droppers or test tubes for convenient testing and analysis.

[0011] In one embodiment, the outer shell includes a top cover, a base, a seat shell, and a seat cover. The top cover is hinged to the base, the seat shell is fixed to the base, and the seat cover is installed on the seat shell so that the seat shell is sealed by the seat cover. This multi-layer structure has a better heat preservation effect, and the base can be designed to be square for easy stacking and not easy to tip over.

[0012] In one embodiment, an insulation layer is provided between the base and the housing. The insulation layer can be made of foam, which has the effect of heat preservation and cushioning.

[0013] In one embodiment, the housing is cylindrical or spherical, and the base is square. The circular structure has a small surface area and does not easily dissipate heat, while the square structure is not easy to tip over and is convenient for multi-layer stacking.

[0014] In one embodiment, the inner shell is provided with a support frame that can limit the position of the tube rack, thus facilitating the positioning of the dropper or test tube.

[0015] In one embodiment, the tube rack has a semi-circular groove on its edge, which is used to install droppers or test tubes. This allows the droppers or test tubes to be installed on the tube rack first, and then the tube rack to be installed on the support frame, making them easier to access.

[0016] In one embodiment, the inner shell is provided with an air extraction port, and the air extraction port is provided with a one-way valve so that the air in the first closed inner cavity of the inner shell can be extracted from the air extraction port to form a negative pressure state. The negative pressure insulation effect is better, and if it is close to a vacuum state, the heat convection can be avoided to prevent temperature transfer.

[0017] It should be understood that the description in this section is not intended to identify key or essential features of the embodiments of this application, nor is it intended to limit the scope of this application. Other features of this application will become readily apparent from the following description. Attached Figure Description

[0018] The above and other objects, features, and advantages of exemplary embodiments of this application will become readily apparent from the following detailed description taken in conjunction with the accompanying drawings. Several embodiments of this application are illustrated in the drawings by way of example and not limitation, in which:

[0019] In the accompanying drawings, the same or corresponding reference numerals indicate the same or corresponding parts.

[0020] Figure 1 This diagram illustrates the off state of the mass spectrometry analysis kit of Embodiment 1 of this application;

[0021] Figure 2 This paper shows a schematic diagram of the open state structure of the mass spectrometry analysis kit of Embodiment 1 of this application;

[0022] Figure 3 A half-sectional schematic diagram of the mass spectrometry analysis kit of Example 1 of this application is shown;

[0023] Figure 4 A schematic diagram of the lower shell structure of the mass spectrometry analysis kit of Embodiment 1 of this application is shown;

[0024] Figure 5 This paper shows a schematic diagram of the dropper installed on the tube rack of the mass spectrometry analysis kit of Embodiment 1 of this application;

[0025] Figure 6 A schematic diagram of the tube rack of the mass spectrometry analysis kit of Example 1 of this application is shown;

[0026] Figure 7 A half-sectional schematic diagram of the dropper of the mass spectrometry analysis kit of Example 1 of this application is shown;

[0027] Figure 8 A half-sectional schematic diagram of the mass spectrometry analysis kit of Example 2 of this application is shown;

[0028] Figure 9 It shows Figure 8 Enlarged diagram of point A in the middle. Detailed Implementation

[0029] To make the objectives, features, and advantages of this application more apparent and understandable, the technical solutions in the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this application, and not all embodiments. Based on the embodiments of this application, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this application.

[0030] like Figure 1 , Figure 2 and Figure 3 As shown, a mass spectrometry analysis kit includes an inner shell 1 and an outer shell 2. The inner shell 1 is provided with a first closed inner cavity 11, and the outer shell 2 is provided with a second closed inner cavity 21. The inner shell 1 is located in the second closed inner cavity 21, and a suspension 6 is provided in the second closed inner cavity 21 so that the inner shell 1 is in a suspended state in the second closed inner cavity 21.

[0031] like Figure 2 and Figure 3 As shown, in one embodiment, an elastic fixing band 3 is provided inside the second closed inner cavity 21 of the outer shell 2. The overall density of the inner shell 1 is greater than the density of the suspension 6, and it is suspended in the second closed inner cavity 21 through the elastic fixing band 3. The inner shell 1 is made of metal material and has a large density, while the outer shell 2 is made of polyamide resin with good heat insulation and glass fiber added.

[0032] like Figure 2 , Figure 3 and Figure 4 As shown, in one embodiment, the outer side of the inner shell 1 is provided with an annular fixing lug 12, and the inner side of the outer shell 2 is provided with a fixing ring 22. The elastic fixing band 3 can be a rubber band or a spring, and one or more bands are used, which are disposed between the fixing ring 22 and the fixing lug 12.

[0033] like Figure 3 , Figure 5 and Figure 6As shown, in one embodiment, the inner shell 1 includes an inner cover 15 and a lower shell 16. The inner cover 15 and the lower shell 16 seal to form a first closed inner cavity 11. The lower shell 16 is provided with a tube rack 4, which has multiple fixing holes of different sizes for placing test tubes or droppers 5. The inner cover 15 and the lower shell 16 are connected by threads.

[0034] like Figure 1 , Figure 2 and Figure 3 As shown, in one embodiment, the outer casing 2 includes an upper cover 23, a base 24, a seat shell 25, and a seat cover 26. The upper cover 23 is hinged to the base 24, forming a sealed space. The seat shell 25 and seat cover 26 are located within this sealed space. The seat shell 25 is fixed to the base 24, and the seat cover 26 is mounted on the seat shell 25, achieving a seal through the seat cover 26. To ensure sealing and cushioning performance, the seat shell 25 and seat cover 26 are made of rubber or plastic with a rubber coating process. They can also be made of foamed material and have embedded metal materials to provide support. The upper cover 23 has a buckle 27, and the base 24 has a hook 28, allowing the upper cover 23 and base 24 to be fixed together via the buckle 27 and hook 28. Alternatively, the upper cover 23 and base 24 can be directly fixed together with tape. The seat shell 25 and seat cover 26 are designed as rotating structures and connected by threads.

[0035] like Figure 2 and Figure 3 As shown, in one embodiment, a heat insulation layer 29 is provided between the base 24 and the housing 25, and the heat insulation layer 29 is made of foam material.

[0036] like Figure 2 and Figure 3 As shown, in one embodiment, the housing 25 is cylindrical or spherical, and the base 24 is square on the outside.

[0037] like Figure 2 and Figure 4 As shown, in one embodiment, the inner shell 1 is provided with a support frame 13, which can restrict the position of the tube rack 4. The support frame 13 is provided with a plurality of fixing slots 14 to fix the position of the dropper 5.

[0038] like Figure 6 and Figure 7 As shown, in one embodiment, the tube rack 4 has a semi-circular groove 41 on its edge, on which a dropper 5 or a test tube is mounted. The dropper 5 may be equipped with a rubber cap 51 and a rubber bladder 52. The rubber bladder 52 is fixed to the end of the dropper 5, and the rubber cap 51 is located at the head of the dropper 5. The reagent can flow back and forth by squeezing the rubber bladder 52 and the rubber cap 51 of the dropper 5. The tube rack 4 is made of a low thermal conductivity foam material or a composite of multiple materials.

[0039] When using, first place the reagent in the first closed inner cavity 11 of the inner shell 1, seal it, place the inner shell 1 in the second closed inner cavity 21, add the suspension 6, and then close the seat cover 26. Rotate the upper cover 23 to make the buckle 27 engage with the hook 28.

[0040] Example 2:

[0041] like Figure 8 As shown, a mass spectrometry analysis kit includes an inner shell 1 and an outer shell 2. The inner shell 1 has a first closed inner cavity 11, and the outer shell 2 has a second closed inner cavity 21. The inner shell 1 is located within the second closed inner cavity 21, and a suspension 6 is provided within the second closed inner cavity 21 to keep the inner shell 1 in a suspended state. Both the inner shell 1 and the outer shell 2 are designed as cylindrical container structures.

[0042] like Figure 9 As shown, in one embodiment, the inner cover 15 of the inner shell 1 is provided with an air extraction port 17. The air extraction port 17 is provided with a one-way valve 18, which allows air in the first closed inner cavity 11 of the inner shell 1 to be extracted from the air extraction port 17 to form a negative pressure state. The one-way valve 18 is provided with a control diaphragm 19, which is covered by a return spring 20 to the air extraction hole 10. When evacuating air, if the tube rack 4 of the inner shell 1 holds a dropper 5, the inner shell 1 needs to be rotated so that the head of the dropper 5 faces upward to facilitate air extraction. The one-way valve 18 can be designed similarly to a tire valve, but with the installation direction reversed. That is, the tire needs to be inflated, but the inner shell 1 needs to be evacuated to form a negative pressure, or even close to a vacuum, thus achieving better heat preservation.

[0043] It should be understood that the various forms of processes shown above can be used to rearrange, add, or delete steps. For example, the steps described in this application can be executed in parallel, sequentially, or in different orders, as long as the desired result of the technical solution of this application can be achieved, and this is not limited herein.

[0044] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one of that feature. In the description of this application, "a plurality of" means two or more, unless otherwise explicitly specified.

[0045] The above description is merely a specific embodiment of this application, but the scope of protection of this application is not limited thereto. Any variations or substitutions that can be easily conceived by those skilled in the art within the scope of the technology disclosed in this application should be included within the scope of protection of this application. Therefore, the scope of protection of this application should be determined by the scope of the claims.

Claims

1. A mass spectrometry analysis kit, characterized in that: It includes an inner shell (1) and an outer shell (2). The inner shell (1) has a first closed inner cavity (11), and the outer shell (2) has a second closed inner cavity (21). The inner shell (1) is located in the second closed inner cavity (21). The second closed inner cavity (21) contains a suspension (6) so that the inner shell (1) is in a suspended state in the second closed inner cavity (21).

2. The mass spectrometry analysis kit according to claim 1, characterized in that: The second closed inner cavity (21) of the outer shell (2) is provided with an elastic fixing band (3). The overall density of the inner shell (1) is greater than the density of the suspension (6) and is suspended in the second closed inner cavity (21) through the elastic fixing band (3).

3. The mass spectrometry analysis kit according to claim 2, characterized in that: The outer side of the inner shell (1) is provided with a fixing ear (12), and the inner side of the outer shell (2) is provided with a fixing ring (22).

4. The mass spectrometry analysis kit according to claim 1, characterized in that: The first closed inner cavity (11) of the inner shell (1) is provided with a tube rack (4).

5. The mass spectrometry analysis kit according to claim 4, characterized in that: The inner shell (1) is provided with a support frame (13), which can limit the position of the tube frame (4).

6. The mass spectrometry analysis kit according to claim 5, characterized in that: The tube rack (4) has a semi-circular groove (41) on its edge, and a dropper (5) or test tube is installed in the semi-circular groove (41).

7. The mass spectrometry analysis kit according to claim 1, characterized in that: The outer casing (2) includes an upper cover (23), a base (24), a seat shell (25), and a seat cover (26). The upper cover (23) is hinged to the base (24), the seat shell (25) is fixed to the base (24), and the seat cover (26) is installed on the seat shell (25) so that the seat shell (25) is sealed by the seat cover (26).

8. The mass spectrometry analysis kit according to claim 7, characterized in that: An insulation layer (29) is provided between the base (24) and the housing (25).

9. The mass spectrometry analysis kit according to claim 8, characterized in that: The housing (25) is cylindrical or spherical, and the base (24) is square on the outside.

10. The mass spectrometry analysis kit according to any one of claims 1-9, characterized in that: The inner shell (1) is provided with an air extraction port (17), and the air extraction port (17) is provided with a one-way valve (18) so that the air in the first closed inner cavity (11) of the inner shell (1) can be extracted from the air extraction port (17) to form a negative pressure state.

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