A flow cytometer filter

Abstract

The utility model relates to a flow cytometer filter, including rotating lid, the rotating lid inner chamber bottom has the rotating plate, the left and right sides of rotating plate near the bottom all are established with the tapered groove, the recess is established with in the top center of tapered groove, the bottom of tapered groove has the triangle guide plate, through the mutual cooperation between rotating lid, central shaft, fixed plate, blanking box, mounting plate, driving motor, rotating plate, triangle guide plate, L type connecting plate, arc shape turnover board, moving link, pasting wheel, clamping block, ring spring, transmission rod, limit plate etc. parts, can realize when needing to filter and wash to several sheath liquid bottles, can fix with several clamping blocks and pasting to sheath liquid bottle, and through starting driving motor drives several sheath liquid bottles and rotates and removes, when sheath liquid bottle bottle mouth is upward, can contact with the washing liquid in the blanking box inside, completes the cleaning to the bottle, and sheath liquid bottle bottle body is turned over simultaneously when washing is completed, and pours out the washing liquid.

Description

Technical Field

[0001] This utility model relates to the field of flow cytometer technology, and in particular to a flow cytometer filter. Background Technology

[0002] Flow cytometers are devices for automated analysis and sorting of cells. They can rapidly measure, store, and display a range of important biophysical and biochemical parameters of dispersed cells suspended in a liquid, and can sort specific cell subpopulations from them based on pre-selected parameter ranges.

[0003] The filters in a flow cytometer mainly include optical filters and fluid filtration systems. The two differ in function and application. Among them, the sheath fluid filter is a key component of the flow cytometer's fluid system. Its core function is to intercept contaminants in the sheath fluid through physical barriers, ensuring the purity and stability of the sheath fluid, thereby ensuring that cells form a stable single-cell laminar flow in the flow chamber.

[0004] Existing sheath fluid filters require the sheath fluid bottle to be cleaned and filtered before use. The existing cleaning and filtration methods all involve manual cleaning of the sheath fluid bottle, which is inconvenient and cannot guarantee the stability and reliability of flow cytometry experiments. Utility Model Content

[0005] The purpose of this invention is to provide a flow cytometer filter to solve the problems mentioned in the background section.

[0006] The present invention relates to a flow cytometer filter, comprising a rotating cover, a rotating plate at the bottom of the inner cavity of the rotating cover, conical grooves on both sides near the bottom of the rotating plate, a groove at the center of the top of the conical grooves, a triangular guide plate at the bottom of the conical grooves, an L-shaped connecting plate fixedly connected to one side of each of the two triangular guide plates, and the two L-shaped connecting plates fixedly connected to the inner wall of the rotating plate on one side of each of the two L-shaped connecting plates, an active motor at the center of the bottom of the rotating plate, a central shaft fixedly connected to the power output shaft of the active motor, the central shaft penetrating the rotating plate and connected to its bearing, and the top of the central shaft penetrating the inner cavity of the rotating cover and fixedly connected thereto.

[0007] In one embodiment, a fixing plate is installed on the outer side of the central shaft near the top, a feeding box is fixedly connected to the right side of the fixing plate, a feeding pipe is provided at the bottom of the feeding box, a pump body is provided in the inner cavity of the feeding pipe, and a proximity trigger is provided at the bottom of the feeding box, the proximity trigger being electrically connected to the pump body.

[0008] In one embodiment, a plurality of movable rods are provided through the inner cavity of the outer side of the rotating cover. The plurality of movable rods are arranged in a circular array with the center of the rotating cover as the center. An arc-shaped flip plate is fixedly connected to one end of each of the movable rods near the central axis.

[0009] In one embodiment, the inner cavity of the arc-shaped flip plate is provided with two transmission rods, which are arranged symmetrically in the horizontal direction. Each of the two transmission rods is fixedly connected to a contact wheel at one end near the central axis. The outer side of the contact wheel is in contact with the bottom of the rotating plate. The end of the transmission rod away from the central axis is fixedly connected to a limiting plate, which is fixedly connected to the arc-shaped flip plate.

[0010] In one embodiment, the mounting plate has an inner cavity, and the mounting plate has circular openings on both the upper and lower sides, with both circular openings communicating with the cavity.

[0011] In one embodiment, the cavity is provided with a plurality of clamping blocks, which are arranged in a circular array with the center of the circular opening as the center, and the bottom of each of the clamping blocks is slidably connected to the cavity.

[0012] In one embodiment, a slot is provided on each of the opposite sides of the clamping blocks, a ring spring is sleeved on the slots, and a sheath liquid bottle is attached to the corresponding side of the clamping blocks.

[0013] The beneficial effects provided by this utility model are:

[0014] 1. Through the cooperation of components such as rotating cap, central shaft, fixed plate, feeding box, mounting plate, drive motor, rotating plate, triangular guide plate, L-shaped connecting plate, arc-shaped flipping plate, moving rod, contacting wheel, clamping block, ring spring, transmission rod, and limiting plate, when several sheath liquid bottles need to be filtered and cleaned, the sheath liquid bottles can be attached and fixed to several clamping blocks, and the drive motor can be started to drive several sheath liquid bottles to rotate and move. When the bottle mouth of the sheath liquid bottle is facing upward, it can contact the cleaning liquid inside the feeding box to complete the cleaning of the bottle. When the cleaning is completed, the bottle body of the sheath liquid bottle is flipped at the same time to pour out the cleaning liquid. Attached Figure Description

[0015] Figure 1 This is a schematic diagram of the structure of this utility model;

[0016] Figure 2 This is a bottom view of the structure of this utility model;

[0017] Figure 3 This is a schematic diagram of the mounting plate structure of this utility model;

[0018] Figure 4 This is a schematic diagram of the rotating cover structure of this utility model;

[0019] Figure 5 This is a schematic diagram of the rotating plate structure of this utility model;

[0020] Figure 6 This is a bottom view of the rotating plate structure of this utility model;

[0021] Figure 7 This is a schematic diagram of the top structure of the mounting plate of this utility model;

[0022] Figure 8 This is an exploded view of the mounting plate structure of this utility model;

[0023] Figure 9 This is a schematic diagram of the clamping block structure of this utility model;

[0024] Figure 10 for Figure 7 Enlarged view of point A in the middle.

[0025] In the attached diagram: 1. Rotating cover; 2. Central shaft; 3. Fixing plate; 4. Feed box; 5. Mounting plate; 6. Drive motor; 7. Sheath liquid bottle; 8. Rotating plate; 9. Triangular guide plate; 10. L-shaped connecting plate; 11. Arc-shaped flipping plate; 12. Moving rod; 13. Adhesion wheel; 14. Clamping block; 15. Ring spring; 16. Transmission rod; 17. Limiting plate. Detailed Implementation

[0026] It should be noted that, unless otherwise specified, the embodiments and features described in these embodiments of the present invention can be combined with each other. The technical solutions of the present invention will be further described below with reference to the accompanying drawings of the embodiments. The present invention is not limited to the specific embodiments described below.

[0027] It should be understood that the same or similar reference numerals in the accompanying drawings of the embodiments correspond to the same or similar components. In the description of this utility model, it should be understood that if terms such as "upper," "lower," "front," "rear," "left," "right," "top," and "bottom" indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, they are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or component referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, the terms describing positional relationships in the accompanying drawings are for illustrative purposes only and should not be construed as limiting this patent. Those skilled in the art can understand the specific meaning of the above terms according to the specific circumstances.

[0028] In one embodiment, such as Figure 1 , Figure 2 , Figure 3 , Figure 4 , Figure 5 , Figure 6 , Figure 7, Figure 8 , Figure 9 and Figure 10 As shown, a flow cytometer filter, such as Figure 1 As shown, the device includes a rotating cover 1, a rotating plate 8 at the bottom of the inner cavity of the rotating cover 1, conical grooves near the bottom on both sides of the rotating plate 8, a groove at the center of the top of the conical groove, a triangular guide plate 9 at the bottom of the conical groove, an L-shaped connecting plate 10 fixedly connected to the corresponding side of the two triangular guide plates 9, and the corresponding side of the two L-shaped connecting plates 10 fixedly connected to the inner wall of the rotating plate 8, an active motor 6 at the bottom center of the rotating plate 8, a central shaft 2 fixedly connected to the power output shaft of the active motor 6, the central shaft 2 passing through the rotating plate 8 and connected to its bearing, the top of the central shaft 2 passing through the inner cavity of the rotating cover 1 and fixedly connected to it, a fixing plate 3 installed near the top of the outer side of the central shaft 2, a feeding box 4 fixedly connected to the right side of the fixing plate 3, a feeding pipe at the bottom of the feeding box 4, a pump body inside the feeding pipe, a proximity trigger at the bottom of the feeding box 4, and the proximity trigger electrically connected to the pump body.

[0029] A plurality of movable rods 12 are provided through the inner cavity of the outer side of the rotating cover 1. The movable rods 12 are arranged in a circular array with the center of the rotating cover 1 as the center. An arc-shaped flip plate 11 is fixedly connected to one end of each movable rod 12 near the central shaft 2. Two transmission rods 16 are provided through the inner cavity of the arc-shaped flip plate 11. The two transmission rods 16 are arranged laterally symmetrically. A contact wheel 13 is fixedly connected to one end of each transmission rod 16 near the central shaft 2. The outer side of the contact wheel 13 is in contact with the bottom of the rotating plate 8. A limit plate 17 is fixedly connected to one end of the transmission rod 16 away from the central shaft 2. The limit plate 17 is fixedly connected to the arc-shaped flip plate 11.

[0030] The mounting plate 5 has an inner cavity, and the mounting plate 5 has round openings on both the top and bottom sides. Both round openings are interconnected with the cavity. The cavity is provided with a number of clamping blocks 14. The clamping blocks 14 are arranged in a circular array with the center of the round opening as the center. The bottom of the clamping blocks 14 is slidably connected to the cavity. The opposite side of the clamping blocks 14 is provided with a slot. The slots are connected together to a ring spring 15. The corresponding side of the clamping blocks 14 is attached to the sheath liquid bottle 7.

[0031] Working principle: First, the operator injects the cleaning agent into the inner cavity of the feeding box 4 and inserts several sheath liquid bottles 7 to be cleaned into adjacent round holes. When the sheath liquid bottles 7 enter the round holes, they are fixed by several clamps 14 and restrained by annular springs 15. When the sheath liquid bottles 7 move below the feeding box 4, a proximity trigger is activated, thereby starting the pump to pour the cleaning agent inside the feeding box 4 into the inner cavity of the sheath liquid bottles 7. When the active motor 6 starts, it drives the central shaft 2 to rotate via the power output shaft. When the central shaft 2 rotates, it drives the rotating cover 1 to rotate. When the rotating cover 1 rotates, it drives several arc-shaped flip plates 11 to rotate via the moving rod 12. When the arc-shaped flip plate 11 moves, it can drive the adjacent bonding wheel 13 to move along the bottom of the rotating plate 8. When the bonding wheel 13 enters the inner cavity of the conical groove, the arc-shaped flip plate 11 can tilt because the top of the conical groove is inclined. When the bonding wheel 13 continues to move and enters the inner cavity of the groove, the groove can generate resistance by bonding with the bonding wheel 13, forcing the arc-shaped flip plate 11 to flip around the current bonding wheel 13. When the arc-shaped flip plate 11 flips, the other bonding wheel 13 contacts and slides with the bottom of the triangular guide plate 9, and the arc-shaped flip plate 11 flips 360 degrees. When the arc-shaped flip plate 11 completes the flip, the cleaning agent in the inner cavity of the sheath liquid bottle 7 can be poured out.

[0032] The technical features of the above embodiments can be combined in any way. For the sake of brevity, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0033] The embodiments described above are merely illustrative of several implementations of this utility model, and while the descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the utility model patent. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of this utility model, and these all fall within the protection scope of this utility model. Therefore, the protection scope of this utility model patent should be determined by the appended claims.

Claims

1. A flow cytometer filter, characterized by, The rotating cover (1) has a rotating plate (8) at the bottom of its inner cavity. The rotating plate (8) has conical grooves on both sides near the bottom. The top center of the conical grooves has a recess. The bottom of the conical grooves has triangular guide plates (9). The corresponding sides of the two triangular guide plates (9) are fixedly connected to L-shaped connecting plates (10). The corresponding sides of the two L-shaped connecting plates (10) are fixedly connected to the inner wall of the rotating plate (8). The bottom center of the rotating plate (8) has an active motor (6). The power output shaft of the active motor (6) is fixedly connected to a central shaft (2). The central shaft (2) passes through the rotating plate (8) and is connected to its bearing. The top of the central shaft (2) passes through the inner cavity of the rotating cover (1) and is fixedly connected to it.

2. A filter for a flow cytometer according to claim 1, wherein, A fixing plate (3) is installed on the outer side of the central shaft (2) near the top. A feeding box (4) is fixedly connected to the right side of the fixing plate (3). A feeding pipe is provided at the bottom of the feeding box (4). A pump body is provided in the inner cavity of the feeding pipe. A proximity trigger is provided at the bottom of the feeding box (4). The proximity trigger is electrically connected to the pump body.

3. A filter for a flow cytometer according to claim 2, wherein, The outer inner cavity of the rotating cover (1) is provided with several moving rods (12). The moving rods (12) are arranged in a circular array with the center of the rotating cover (1) as the center. An arc-shaped flip plate (11) is fixedly connected to one end of each moving rod (12) near the central axis (2).

4. A filter for a flow cytometer according to claim 3, wherein, The inner cavity of the arc-shaped flip plate (11) is provided with two transmission rods (16). The two transmission rods (16) are arranged symmetrically in the horizontal direction. The end of each transmission rod (16) near the central shaft (2) is fixedly connected to a contact wheel (13). The outer side of the contact wheel (13) is in contact with the bottom of the rotating plate (8). The end of the transmission rod (16) away from the central shaft (2) is fixedly connected to a limiting plate (17). The limiting plate (17) is fixedly connected to the arc-shaped flip plate (11).

5. A filter for a flow cytometer according to claim 4, wherein, Each of the movable rods (12) has an installation plate (5) installed at the other end. The installation plate (5) has a cavity inside. The installation plate (5) has round openings on both the upper and lower sides. Both round openings are interconnected with the cavity.

6. A filter for a flow cytometer according to claim 5, wherein, The cavity is provided with a number of clamping blocks (14), which are arranged in a circular array with the center of the circular opening as the center, and the bottom of each clamping block (14) is slidably connected to the cavity.

7. A filter for a flow cytometer according to claim 6, wherein, Each of the clamping blocks (14) has a slot on one side opposite to the others, and a ring spring (15) is sleeved on the same side of each of the clamping blocks (14). A sheath liquid bottle (7) is attached to the corresponding side of each of the clamping blocks (14).

Images