A urine stem cell multi-stage constant-temperature freezing storage device

Abstract

The utility model relates to urine stem cell cryopreservation technical field, especially urine stem cell multistage constant temperature cryopreservation device, including cryopreservation device main part, sealing cover, urine stem cell storage bag placing bin, first baffle, second baffle, positioning bottom box, base and controller, the upper end sealing connection of cryopreservation device main part has sealing cover, and the upper end of cryopreservation device main part is fixed with urine stem cell storage bag placing bin, the utility model discloses through first baffle and second baffle and makes the high, medium, low temperature cavity in cryopreservation device main part, to reduce the influence of high temperature to internal urine stem cell, to realize multistage low temperature environment, still utilize the drive motor in positioning assembly and drive first, second helical gear can synchronous drive first even board and second even board rotate in low, moderate low temperature cavity, forcedly agitate cold air and form turbulent flow, eliminate local cold spot and temperature uneven phenomenon, and then significantly improve the temperature uniformity of cryopreservation device.

Description

Technical Field

[0001] This utility model relates to the field of urine stem cell cryopreservation technology, and in particular to a multi-level constant temperature cryopreservation device for urine stem cells. Background Technology

[0002] As a non-invasive biological sample, urine has the natural advantages of unlimited stem cell sources, convenient access, and minimal ethical controversy. Compared to stem cell sources such as bone marrow and adipose tissue that require invasive procedures, urine stem cell collection is painless and non-invasive, making it especially suitable for children, the elderly, and people with special physical conditions. This significantly expands the coverage of stem cell donors and lays the foundation for the popularization of cell therapy technology. Currently, cryopreservation technology is used to achieve long-term viability preservation of urine stem cells.

[0003] However, existing constant temperature cryopreservation devices generally add an isolation protection zone outside the cryopreservation structure and directly introduce refrigerant into this area. This method is a static isolation structure, which is prone to causing local temperature differences and poor temperature uniformity in the cavity, thus failing to maintain the steady-state conditions required for cryopreservation. Utility Model Content

[0004] To overcome the problem of poor temperature uniformity in urine stem cell cryopreservation devices, this invention provides a multi-stage constant temperature cryopreservation device for urine stem cells.

[0005] The technical solution is as follows: A multi-stage constant temperature cryopreservation device for urinary stem cells includes a cryopreservation device body, a sealing cover, a urinary stem cell storage bag placement chamber, a first isolation plate, a second isolation plate, a positioning base, a base, and a controller. The upper end of the cryopreservation device body is sealed with the sealing cover. The urinary stem cell storage bag placement chamber is fixedly connected to the upper end of the cryopreservation device body. The first isolation plate and the second isolation plate are fixedly connected to the upper end of the cryopreservation device body. The positioning base is fixedly connected to the lower end of the cryopreservation device body. A positioning component is provided inside the positioning base. The positioning component includes a first slider and a second slider. A first uniform plate is fixed to the upper end of the first slider, and a second uniform plate is fixed to the upper end of the second slider. The base is fixed to the lower end of the cryopreservation device body. The controller is installed on the left side of the base. An inspection door is connected to the rear end of the base. An auxiliary component is provided at the upper end of the base. The auxiliary component includes a first output pipe and a second output pipe. A core refrigeration component is provided at the upper end of the base. The core refrigeration component includes an output pipe.

[0006] Furthermore, cavities are provided on the inner sides of both the first and second isolation plates, and the cavities are filled with argon inert gas.

[0007] Furthermore, a deep cryogenic cavity is formed between the urine stem cell storage bag placement chamber and the first isolation plate, a medium cryogenic cavity is formed between the first and second isolation plates, and a low cryogenic cavity is formed between the second isolation plate and the main body of the cryopreservation device.

[0008] Furthermore, the positioning assembly also includes a drive motor. The drive motor is installed inside the positioning base box, and an output shaft is connected to the rear end of the drive motor. A first helical gear is fixed on the surface of the output shaft, and a second helical gear is fixed on the surface of the output shaft.

[0009] Furthermore, the upper end of the first helical gear is connected to a first helical gear ring, which meshes with the first helical gear. A first slider is fixed to the upper end of the first helical gear ring. The upper end of the second helical gear is connected to a second helical gear ring, which meshes with the second helical gear. A second slider is fixed to the upper end of the second helical gear ring.

[0010] Furthermore, a first track is fixed to the bottom of the low-temperature cavity, and the first track is slidably connected to the first slider; a second track is fixed to the bottom of the medium-temperature cavity, and the second track is slidably connected to the second slider.

[0011] Furthermore, the auxiliary components also include a refrigeration unit, with a first output pipe fixedly connected to the front end of the refrigeration unit, and a second output pipe fixedly connected to the upper end of the first output pipe. Solenoid valves are installed at the upper ends of both the second output pipe and the first output pipe.

[0012] Furthermore, the core refrigeration component also includes a liquid nitrogen storage tank. The liquid nitrogen storage tank is connected to the upper end of the base, and a liquid pump is installed at the upper end of the liquid nitrogen storage tank. An output pipe is fixed to the upper end of the liquid pump.

[0013] The beneficial effects of this utility model are:

[0014] This multi-level cryopreservation device for urinary stem cells creates high, medium, and low temperature chambers within the main body of the device through a first and second isolation plate. This reduces the impact of high temperatures on the internal urinary stem cells, thereby achieving a multi-level low temperature environment. Furthermore, filling the first and second isolation plates with argon gas effectively reduces heat conduction, maintaining temperature stability in each zone. Additionally, the drive motor in the positioning component drives the first and second helical gears to synchronously rotate the first and second uniform plates within the low and medium temperature chambers, forcibly agitating the cold gas to create turbulence, eliminating localized cold spots and temperature inhomogeneity, and significantly improving the temperature uniformity of the cryopreservation device. Attached Figure Description

[0015] Figure 1 This is a schematic diagram of the overall structure of the cryopreservation device of this utility model. Figure 1 ;

[0016] Figure 2This is a schematic diagram of the overall structure of the cryopreservation device of this utility model. Figure 2 ;

[0017] Figure 3 This is an exploded view of the cryopreservation device of this utility model;

[0018] Figure 4 This is a half-sectional view of the cryopreservation device of this utility model;

[0019] Figure 5 This is a partially enlarged structural diagram of part A of this utility model.

[0020] Explanation of reference numerals in the attached drawings: 1-Cryopreservation device main body, 2-Sealing cover, 3-Urine stem cell storage bag placement chamber, 4-First isolation plate, 5-Second isolation plate, 6-Cavity, 7-Positioning base box, 8-Positioning component, 9-First homogenizing plate, 10-Second homogenizing plate, 11-Base, 12-Controller, 13-Inspection door, 14-Auxiliary component, 15-Core refrigeration component, 801-Drive motor, 802-Output shaft, 803-First helical gear, 804-Second helical gear, 805-First helical gear ring, 806-Second helical gear ring, 807-First slider, 808-First track, 809-Second slider, 810-Second track, 1401-Refrigerator, 1402-First output pipe, 1403-Second output pipe, 1404-Solenoid valve, 1501-Liquid nitrogen storage tank, 1502-Liquid pump, 1503-Output pipe. Detailed Implementation

[0021] The present invention will now be described in detail with reference to the accompanying drawings and specific embodiments.

[0022] Example 1

[0023] like Figures 1-5As shown, a multi-stage constant-temperature cryopreservation device for urinary stem cells includes a cryopreservation device body 1, a sealing cover 2, a urinary stem cell storage bag placement chamber 3, a first isolation plate 4, a second isolation plate 5, a positioning base box 7, a base 11, and a controller 12. The sealing cover 2 is sealed to the upper end of the cryopreservation device body 1. The urinary stem cell storage bag placement chamber 3 is fixed to the upper end of the cryopreservation device body 1. The first isolation plate 4 and the second isolation plate 5 are fixed to the upper end of the cryopreservation device body 1. The positioning base box 7 is fixed to the lower end of the cryopreservation device body 1. A positioning component 8 is provided inside the positioning base box 7. The positioning component 8 includes a first slider 807 and a second slider 809. A first uniform plate 9 is fixed to the upper end of the first slider 807, and a second uniform plate 9 is fixed to the upper end of the second slider 809. The lower end of the cryopreservation device body 1 is fixed with a base 11, a controller 12 is installed on the left side of the base 11, and an inspection door 13 is connected to the rear end of the base 11. An auxiliary component 14 is provided at the upper end of the base 11. The auxiliary component 14 includes a first output pipe 1402 and a second output pipe 1403. A core refrigeration component 15 is provided at the upper end of the base 11. The core refrigeration component 15 includes an output pipe 1503. The urine stem cell storage bag placement chamber 3 serves as the core cryopreservation container of the cryopreservation device body 1. Temperature zones are divided by the first isolation plate 4 and the second isolation plate 5 to form multi-stage cooling and reduce external environmental interference. The positioning component 8 can simultaneously drive the first uniform plate 9 and the second slider 809 to rotate and move within the cryopreservation device body 1, thereby reducing local temperature differences.

[0024] The inner sides of the first isolation plate 4 and the second isolation plate 5 are both provided with cavities 6, which are filled with argon inert gas. The argon filling effectively reduces heat conduction in order to maintain the temperature stability of each temperature zone.

[0025] A deep cryogenic cavity is formed between the urine stem cell storage bag placement chamber 3 and the first isolation plate 4, a medium cryogenic cavity is formed between the first isolation plate 4 and the second isolation plate 5, and a low cryogenic cavity is formed between the second isolation plate 5 and the cryopreservation device body 1. Through the layered design of the isolation plates, multi-level cryopreservation function is realized in a compact space, and damage to urine stem cells in the deep cryogenic cavity is avoided.

[0026] The positioning assembly 8 also includes a drive motor 801. The drive motor 801 is installed inside the positioning base box 7. The rear end of the drive motor 801 is connected to an output shaft 802. A first helical gear 803 and a second helical gear 804 are fixed on the surface of the output shaft 802. By starting the drive motor 801, the first helical gear 803 and the second helical gear 804 on the output shaft 802 can be rotated to realize the movement of the first uniform plate 9 and the second slider 809.

[0027] The upper end of the first helical gear 803 is connected to the first helical gear ring 805, which meshes with the first helical gear 803. The upper end of the first helical gear ring 805 is fixed with the first slider 807. The upper end of the second helical gear 804 is connected to the second helical gear ring 806, which meshes with the second helical gear 804. The upper end of the second helical gear ring 806 is fixed with the second slider 809. The rotation of the first helical gear 803 can drive the first helical gear ring 805 to rotate, and the rotation of the second helical gear 804 can drive the second helical gear ring 806 to rotate, so as to drive the first slider 807 and the second slider 809 to adjust their positions.

[0028] The bottom end of the low-temperature cavity is fixed with a first track 808, which is slidably connected to a first slider 807. The bottom end of the medium-temperature cavity is fixed with a second track 810, which is slidably connected to a second slider 809. The first slider 807 moves in the first track 808, thereby ensuring the stable movement of the first uniform plate 9. The second slider 809 moves in the second track 810, thereby ensuring the stable movement of the second uniform plate 10, thus serving as a guide.

[0029] During operation, by starting the drive motor 801, the first helical gear 803 and the second helical gear 804 on the output shaft 802 are driven to rotate, thereby driving the first helical gear ring 805 and the second helical gear ring 806 to rotate synchronously. This allows the first slider 807 to slide in the first track 808 and the second slider 809 to rotate in the second track 810. This enables the first uniform plate 9 to rotate stably in the low-temperature cavity, and similarly, the second uniform plate 10 to rotate stably in the medium-temperature cavity, thereby achieving uniformity of cold air in the low-temperature cavity and the medium-temperature cavity.

[0030] Example 2

[0031] Based on Example 1, such as Figures 3-4 As shown, the auxiliary component 14 also includes a refrigerator 1401. A first output pipe 1402 is fixedly connected to the front end of the refrigerator 1401, and a second output pipe 1403 is fixedly connected to the upper end of the first output pipe 1402. Solenoid valves 1404 are installed at the upper ends of both the second output pipe 1403 and the first output pipe 1402. By starting the refrigerator 1401, it is convenient to input cold air into the medium-low temperature cavity and the low-low temperature cavity.

[0032] The core refrigeration component 15 also includes a liquid nitrogen storage tank 1501. The upper end of the base 11 is connected to the liquid nitrogen storage tank 1501. A liquid pump 1502 is installed on the upper end of the liquid nitrogen storage tank 1501. An output pipe 1503 is fixedly connected to the upper end of the liquid pump 1502. By starting the liquid pump 1502, the liquid nitrogen in the liquid nitrogen storage tank 1501 can be easily extracted.

[0033] It is also considered that by opening the solenoid valves 1404 on the first output pipe 1402 and the second output pipe 1403 through the controller 12, the refrigerator 1401 is started to provide active cooling to the medium and low temperature range, so as to form a low temperature environment on the outer periphery of the first isolation plate 4, which is conducive to storage. In addition, the liquid nitrogen storage tank 1501 delivers liquid nitrogen to the deep low temperature cavity through the liquid pump 1502, thereby achieving ultra-low temperature preservation of urine stem cells in the urine stem cell storage bag placement chamber 3 at -196°C.

[0034] The above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. A multi-stage constant-temperature cryopreservation device for urine stem cells, comprising a cryopreservation device body (1); characterized in that: It also includes a sealing cap (2), a urine stem cell storage bag placement chamber (3), a first isolation plate (4), a second isolation plate (5), a positioning base box (7), a base (11), and a controller (12). The upper end of the cryopreservation device body (1) is sealed with a sealing cap (2). The upper end of the cryopreservation device body (1) is fixedly connected with a urine stem cell storage bag placement chamber (3). The upper end of the cryopreservation device body (1) is fixedly connected with a first isolation plate (4). The upper end of the cryopreservation device body (1) is fixedly connected with a second isolation plate (5). The lower end of the cryopreservation device body (1) is fixedly connected with a positioning base box (7). The inner side of the positioning base box (7) is provided with a positioning component (8). The positioning component (8) includes a first slider ( 807) and the second slider (809), the upper end of the first slider (807) is fixed with a first uniform plate (9), the upper end of the second slider (809) is fixed with a second uniform plate (10), the lower end of the cryopreservation device body (1) is fixed with a base (11), the left side of the base (11) is equipped with a controller (12), the rear end of the base (11) is connected with an inspection door (13), the upper end of the base (11) is provided with an auxiliary component (14), the auxiliary component (14) includes a first output pipe (1402) and a second output pipe (1403), the upper end of the base (11) is provided with a core refrigeration component (15), the core refrigeration component (15) includes an output pipe (1503).

2. The multi-stage constant-temperature cryopreservation device for urine stem cells according to claim 1, characterized in that: Both the first isolation plate (4) and the second isolation plate (5) have cavities (6) on their inner sides, and the cavities (6) are filled with argon inert gas.

3. The multi-stage constant-temperature cryopreservation device for urine stem cells according to claim 1, characterized in that: A deep low-temperature cavity is formed between the urine stem cell storage bag placement chamber (3) and the first isolation plate (4), a medium low-temperature cavity is formed between the first isolation plate (4) and the second isolation plate (5), and a low-temperature cavity is formed between the second isolation plate (5) and the cryopreservation device body (1).

4. The multi-stage constant-temperature cryopreservation device for urine stem cells according to claim 1, characterized in that: The positioning assembly (8) also includes a drive motor (801). The drive motor (801) is installed inside the positioning base box (7). The rear end of the drive motor (801) is connected to an output shaft (802). A first helical gear (803) is fixed on the surface of the output shaft (802), and a second helical gear (804) is fixed on the surface of the output shaft (802).

5. The multi-stage constant-temperature cryopreservation device for urine stem cells according to claim 4, characterized in that: The upper end of the first helical gear (803) is connected to the first helical gear ring (805), which meshes with the first helical gear (803). The upper end of the first helical gear ring (805) is fixed with the first slider (807). The upper end of the second helical gear (804) is connected to the second helical gear ring (806), which meshes with the second helical gear (804). The upper end of the second helical gear ring (806) is fixed with the second slider (809).

6. The multi-stage constant-temperature cryopreservation device for urine stem cells according to claim 3, characterized in that: The bottom end of the low-temperature cavity is fixed with a first track (808), which is slidably connected to the first slider (807). The bottom end of the medium-temperature cavity is fixed with a second track (810), which is slidably connected to the second slider (809).

7. The multi-stage constant-temperature cryopreservation device for urine stem cells according to claim 1, characterized in that: The auxiliary component (14) also includes a refrigerator (1401), a first output pipe (1402) is fixedly connected to the front end of the refrigerator (1401), a second output pipe (1403) is fixedly connected to the upper end of the first output pipe (1402), and a solenoid valve (1404) is installed at the upper end of both the second output pipe (1403) and the first output pipe (1402).

8. The multi-stage constant-temperature cryopreservation device for urine stem cells according to claim 1, characterized in that: The core refrigeration component (15) also includes a liquid nitrogen storage tank (1501), the upper end of the base (11) is connected to the liquid nitrogen storage tank (1501), the upper end of the liquid nitrogen storage tank (1501) is equipped with a liquid pump (1502), and the upper end of the liquid pump (1502) is fixedly connected to an output pipe (1503).

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