An inverted microscope multicolor fluorescence illumination device

Abstract

The utility model discloses an inverted microscope multicolor fluorescence illumination device, including microscope body, the front end of microscope body is provided with the focusing knob, the top left side of microscope body is connected with the observation cylinder, the top of observation cylinder is installed with the ocular lens, the top middle section of microscope body is equipped with the accommodation groove, the upper end of accommodation groove is connected with the object table, the inner chamber bottom of accommodation groove is installed with the objective lens, the lower extreme of accommodation groove is provided with the fluorescence filter component, the top right side of microscope body is connected with the stand, the cross end of stand is connected with the light collector, the right end surface bottom of microscope body is connected with T type rail, the outside of T type rail is equipped with the supporting plate, the top of supporting plate is connected with the illumination box, the convenience of multicolor fluorescence observation is greatly promoted, is favorable for improving the imaging quality of fluorescence image to obtain clearer, reliable observation result.

Description

Technical Field

[0001] This utility model relates to the field of microscope technology, and in particular to a multicolor fluorescence illumination device for an inverted microscope. Background Technology

[0002] A microscope is an effective tool for observing samples at the microscopic scale. Among these, observing cells is a primary imaging sample used in biomedical applications. However, during cell sample observation, cells often adhere to the substrate, hindering proper observation. In an inverted microscope, by inverting the microscope objective, adherent cells can be observed more effectively. However, cells often have high transparency, necessitating the use of fluorescence imaging to improve the contrast of the cell image.

[0003] The object is illuminated to emit fluorescence, and its shape and location are then observed under a microscope. However, existing inverted microscopes are not easy to install and remove the illumination source, making maintenance or repair cumbersome and inconvenient to use. To overcome these disadvantages, this invention provides a multicolor fluorescence illumination device for inverted microscopes. Utility Model Content

[0004] The purpose of this invention is to address the shortcomings of existing technologies by proposing a multicolor fluorescence illumination device for an inverted microscope.

[0005] To achieve the above objectives, the present invention adopts the following technical solution:

[0006] A multicolor fluorescence illumination device for an inverted microscope includes a microscope body, a focusing knob at the front end of the microscope body, an observation tube connected to the top left side of the microscope body, an eyepiece mounted on the top of the observation tube, a receiving groove in the middle section of the top of the microscope body, a stage connected to the upper end of the receiving groove, an objective lens mounted at the bottom of the inner cavity of the receiving groove, a fluorescence filter assembly at the lower end of the receiving groove, a stand connected to the top right side of the microscope body, a condenser connected to the horizontal end of the stand, a T-shaped rail connected to the bottom of the right end face of the microscope body, a support plate sleeved on the outside of the T-shaped rail, and an illumination box connected to the top of the support plate.

[0007] Preferably, the fluorescence filter assembly includes a filter chamber, the right end of which is connected to a light-passing hole, a reflector connected to the bottom of the inner cavity of the filter chamber, an excitation filter vertically installed on the right side of the inner cavity of the filter chamber, an emission filter horizontally installed in the middle of the inner cavity of the filter chamber, a dichroic separator between the excitation filter and the emission filter, the included angle between the excitation filter, the emission filter and the dichroic separator is 45 degrees, and a visual channel is opened between the left end of the filter chamber and the observation tube.

[0008] Preferably, a focusing lens is embedded in the lower part of the left side wall of the lighting box, a horizontal shaft is rotatably connected to the upper part of the inner cavity of the lighting box, a worm gear is connected to the right side of the outer wall of the horizontal shaft, a linkage rod is connected to the left side of the outer wall of the horizontal shaft, an arc-shaped lamp plate is connected to the lower end of the linkage rod, multiple light source channels are opened on the arc-shaped lamp plate, and LED beads of different colors are respectively installed on the right side of the inner cavity of the multiple light source channels, a crank is rotatably connected to the top of the lighting box, a worm is connected to the lower end of the crank, the worm meshes with a worm wheel, and heat dissipation fins are embedded in the front and rear of the outer wall of the lighting box.

[0009] Preferably, a T-shaped groove is provided on the bottom left side of the support plate, a storage battery is connected to the bottom right side of the support plate, and a control switch is connected to the outer wall of the support plate. The control switch is electrically connected to the LED beads and the storage battery.

[0010] Preferably, the unfolding helix angle of the worm is smaller than the friction angle of the worm wheel contact.

[0011] Preferably, the light-emitting aperture, the condenser lens, and the lower light source channel are arranged along the same axis.

[0012] The beneficial effects of this utility model are:

[0013] This technical solution has a reasonable structural design, realizing convenient and efficient multicolor fluorescence excitation and switching. By setting an arc-shaped lamp plate with multiple LED beads of different colors and using a worm gear transmission mechanism for adjustment, users can effectively switch excitation light of different wavelengths, greatly improving the convenience of multicolor fluorescence observation.

[0014] In this technical solution, the support plate is detachable and easy to inspect and maintain the lighting box. By setting the condenser lens, light aperture and light source channel coaxially, and integrating the excitation filter, dichroic filter and emission filter into a filter assembly, the convergence of excitation light and the separation of fluorescence signal are ensured, which helps to improve the imaging quality of fluorescence image, thereby obtaining clearer and more reliable observation results. Attached Figure Description

[0015] To more clearly illustrate the technical solution of this utility model, the drawings used in the description of the specific embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0016] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0017] Figure 2 This is a schematic diagram of the internal structure of the present invention;

[0018] Figure 3 This is a schematic diagram of the fluorescent filter component in this utility model;

[0019] Figure 4 This is a side view of the arc-shaped lamp panel in this utility model.

[0020] The attached figures are labeled as follows:

[0021] 1. Microscope body; 2. Focusing knob; 3. Observation tube; 4. Eyepiece; 5. Receiving slot; 6. Stage; 7. Objective lens; 8. Fluorescence filter assembly; 9. Stand; 10. Condenser; 11. T-rail; 12. Support plate; 13. Illumination box;

[0022] 81. Filter chamber; 82. Light aperture; 83. Reflector; 84. Excitation filter; 85. Emission filter; 86. Dichroic filter; 87. Visual channel;

[0023] 121. T-slot; 122. Battery; 123. Control switch;

[0024] 131. Condensing lens; 132. Horizontal axis; 133. Worm gear; 134. Linkage rod; 135. Curved lamp panel; 136. Light source channel; 137. LED lamp; 138. Crank handle; 139. Worm gear; 130. Heat sink fins. Detailed Implementation

[0025] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those skilled in the art without creative effort are within the protection scope of the present utility model.

[0026] like Figure 1-4 As shown, a multicolor fluorescence illumination device for an inverted microscope is disclosed, comprising a microscope body 1, a focusing knob 2 at the front end of the microscope body 1, an observation tube 3 connected to the top left side of the microscope body 1, an eyepiece 4 mounted on the top of the observation tube 3, a receiving groove 5 in the middle of the top of the microscope body 1, a stage 6 connected to the upper end of the receiving groove 5, an objective lens 7 mounted at the bottom of the inner cavity of the receiving groove 5, a fluorescence filter assembly 8 at the lower end of the receiving groove 5, a stand 9 connected to the top right side of the microscope body 1, a condenser 10 connected to the horizontal end of the stand 9, a T-rail 11 connected to the bottom of the right end face of the microscope body 1, a support plate 12 sleeved on the outside of the T-rail 11, and an illumination box 13 connected to the top of the support plate 12.

[0027] The fluorescence filter assembly 8 includes a filter chamber 81, with a light through-hole 82 connected to the right end of the filter chamber 81. A reflector 83 is connected to the bottom of the inner cavity of the filter chamber 81. An excitation filter 84 is vertically installed on the right side of the inner cavity of the filter chamber 81, and an emission filter 85 is horizontally installed in the middle of the inner cavity of the filter chamber 81. A dichroic separator 86 is provided between the excitation filter 84 and the emission filter 85. The included angles between the excitation filter 84, the emission filter 85 and the dichroic separator 86 are all 45 degrees. A visual channel 87 is provided between the left end of the filter chamber 81 and the observation tube 3. The fluorescence filter assembly 8 is a key module that determines the quality of fluorescence imaging. Its core function is to filter stray light and separate the excitation light and the fluorescence signal.

[0028] A focusing lens 131 is embedded in the lower part of the left side wall of the lighting box 13. A horizontal shaft 132 is rotatably connected to the upper part of the inner cavity of the lighting box 13. A worm gear 133 is connected to the right side of the outer wall of the horizontal shaft 132. A linkage rod 134 is connected to the left side of the outer wall of the horizontal shaft 132. An arc-shaped lamp plate 135 is connected to the lower end of the linkage rod 134. Multiple light source channels 136 are opened on the arc-shaped lamp plate 135. LED beads 137 of different colors are installed on the right side of the inner cavity of the multiple light source channels 136. A crank 138 is rotatably connected to the top of the lighting box 13. A worm gear 139 is connected to the lower end of the crank 138. The worm gear 139 meshes with the worm gear 133. Heat dissipation fins 130 are embedded in the front and rear of the outer wall of the lighting box 13.

[0029] A T-slot 121 is provided on the bottom left side of the support plate 12. The T-slot 121 cooperates with the T-rail 11 to realize the quick installation and disassembly of the lighting box 13, which facilitates maintenance work such as replacing LED beads 137 and cleaning the condenser lens 131. A battery 122 is connected to the bottom right side of the support plate 12. A control switch 123 is connected to the outer wall of the support plate 12. The control switch 123 is electrically connected to the LED beads 137 and the battery 122.

[0030] The unfolded helix angle of the worm 139 is smaller than the friction angle of the worm wheel 133 and the worm 139 in contact, which can achieve self-locking and prevent the curved lamp plate 135 from shaking.

[0031] The light-emitting aperture 82, the condenser lens 131, and the lower light source channel 136 are arranged on the same axis to ensure effective access of the lighting light.

[0032] The specific implementation method of this embodiment is as follows:

[0033] In use, first check that the entire device is intact. Insert the T-rail 11 into the T-slot 121 to complete the installation of the support plate 12, ensuring that the light through-hole 82, the condenser lens 131, and the lower light source channel 136 are coaxial. Place the sample to be observed on the stage 6. After closing the control switch 123, the LED bead 137 is powered on and illuminates. Use the crank 138 to drive the worm gear 139 to rotate. The worm gear 139 meshes with the transmission worm wheel 133, causing the horizontal axis 132 to drive the linkage rod 134 to deflect. This allows adjustment of the corresponding positions of the curved lamp plate 135 and the condenser lens 131, ensuring the corresponding light source channel... Different colored LED beads 137 inside 136 are used as excitation light sources. The excitation light source is focused by the condenser lens 131 to achieve the light intensity required to excite fluorescence. The light enters the filter chamber 81 through the light through hole 82, and is filtered by the excitation filter 84 to obtain light of a specific short wavelength. After being reflected by the dichroic filter 86, it is projected onto the observation sample through the objective lens 7. After being excited on the sample, the emitted wavelength is greater than the spectrum of the excitation light. Then, it passes through the objective lens 7, the dichroic filter 86 and the emission filter 85, and is reflected by the reflector 83 before entering the eyepiece 4 at the top of the observation tube 3 through the visual channel 87.

[0034] The preferred embodiments of this utility model disclosed above are merely illustrative of the present utility model. These preferred embodiments do not exhaustively describe all details, nor do they limit the utility model to any specific implementation. Clearly, many modifications and variations can be made based on the content of this specification. This specification selects and specifically describes these embodiments to better explain the principles and practical applications of this utility model, thereby enabling those skilled in the art to better understand and utilize it. This utility model is limited only by the claims and their full scope and equivalents.

Claims

1. An inverted microscope multicolor fluorescence illumination device comprising a microscope body (1), characterized in that: The microscope body (1) has a focusing knob (2) at the front end. The microscope body (1) has an observation tube (3) connected to the top left side. An eyepiece (4) is installed on the top of the observation tube (3). The microscope body (1) has a receiving groove (5) in the middle of the top. A stage (6) is connected to the upper end of the receiving groove (5). An objective lens (7) is installed at the bottom of the inner cavity of the receiving groove (5). A fluorescence filter assembly (8) is provided at the lower end of the receiving groove (5). A stand (9) is connected to the top right side of the microscope body (1). A condenser (10) is connected to the horizontal end of the stand (9). A T-rail (11) is connected to the bottom of the right end face of the microscope body (1). A support plate (12) is fitted on the outside of the T-rail (11). An illumination box (13) is connected to the top of the support plate (12).

2. The inverted microscope multicolor fluorescence illumination device of claim 1, wherein: The fluorescence filter assembly (8) includes a filter chamber (81), with a light through hole (82) connected to the right end of the filter chamber (81). A reflector (83) is connected to the bottom of the inner cavity of the filter chamber (81). An excitation filter (84) is vertically installed on the right side of the inner cavity of the filter chamber (81). An emission filter (85) is horizontally installed in the middle of the inner cavity of the filter chamber (81). A dichroic separator (86) is provided between the excitation filter (84) and the emission filter (85). The included angle between the excitation filter (84), the emission filter (85), and the dichroic separator (86) is 45 degrees. A visual channel (87) is provided between the left end of the filter chamber (81) and the observation tube (3).

3. The inverted microscope multicolor fluorescence illumination device of claim 2, wherein: A focusing lens (131) is embedded in the lower part of the left side wall of the lighting box (13). A horizontal shaft (132) is rotatably connected to the upper part of the inner cavity of the lighting box (13). A worm gear (133) is connected to the right side of the outer wall of the horizontal shaft (132). A linkage rod (134) is connected to the left side of the outer wall of the horizontal shaft (132). An arc-shaped lamp plate (135) is connected to the lower end of the linkage rod (134). Multiple light source channels (136) are opened on the arc-shaped lamp plate (135). LED beads (137) of different colors are installed on the right side of the inner cavity of the multiple light source channels (136). A crank (138) is rotatably connected to the top of the lighting box (13). A worm (139) is connected to the lower end of the crank (138). The worm (139) meshes with the worm gear (133). Heat dissipation fins (130) are embedded in the front and back of the outer wall of the lighting box (13).

4. The inverted microscope multicolor fluorescence illumination device of claim 3, wherein: A T-shaped groove (121) is provided on the bottom left side of the support plate (12), and a storage battery (122) is connected to the bottom right side of the support plate (12). A control switch (123) is connected to the outer wall of the support plate (12), and the control switch (123) is electrically connected to the LED lamp bead (137) and the storage battery (122).

5. The inverted microscope multicolor fluorescence illumination device of claim 3, wherein: The unfolded helix angle of the worm (139) is smaller than the friction angle of the worm wheel (133) and worm (139) contact.

6. The inverted microscope multicolor fluorescence illumination device of claim 3, wherein: The light ray through hole (82), the condenser lens (131) and the lower light source passage (136) are coaxial.

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