An enrichment device and system for exosomes or viruses
By designing an enrichment device that includes a shell unit and multiple layers of retention units, the problem of easy clogging in existing exosome or virus enrichment devices has been solved, achieving efficient and continuous particle separation and recovery, and ensuring high purity and low pollution enrichment effect.
Patent Information
- Application Number
- CN202522450959.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-11-19
- Publication Date
- 2026-11-03
- Estimated Expiration
- 2035-11-19
Smart Images
Figure CN224930528U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of biotechnology, and in particular to an enrichment device and system for exosomes or viruses. Background Technology
[0002] Exosomes, as nanoscale vesicles secreted by cells, carry viral particles that are of significant value in biomedical fields such as disease diagnosis, pathological mechanism research, and vaccine development. Enrichment of exosomes or viruses is a crucial prerequisite for subsequent detection and analysis, requiring the isolation of target viral particles from complex liquid samples (such as blood, body fluids, and cell culture media) while removing interfering substances such as contaminating proteins and cell debris. With the development of biotechnology, the demand for high-purity, high-recovery exosome or virus enrichment is increasingly urgent. In clinical diagnostic scenarios, the concentration of target viruses in samples is often extremely low, necessitating efficient enrichment methods to improve detection sensitivity. In research scenarios, the enrichment process must avoid damaging the activity of target particles to ensure the accuracy of subsequent functional analysis. Current enrichment technologies need to be adaptable to the characteristics of samples from different sources, balancing separation efficiency and ease of operation. They must rapidly separate target particles from impurities while simplifying procedures and reducing the risk of cross-contamination to meet the needs of large-scale sample processing and precise experiments.
[0003] Currently, the industry mainly employs two types of technologies for target particle enrichment: one is two-stage filtration technology using sandwich flat sheet membrane structures, which separates target particles from impurities through the graded retention of the double-layer membrane; the other is hollow fiber membrane reactor technology, which has the advantage of easy scale-up due to its continuous flow characteristics. However, sandwich flat sheet membrane structures face problems in practical applications such as complex encapsulation and easy clogging of the retention zone, while hollow fiber membrane reactors, due to their single membrane pore size design, struggle to achieve selective retention and efficient recovery of target particles.
[0004] Currently, no effective solution has been proposed for the problems of easy clogging and impaired interception effect in related technologies. Utility Model Content
[0005] The purpose of this invention is to address the shortcomings of existing technologies by providing an enrichment device and system for exosomes or viruses, thereby solving the problems of easy clogging and reduced retention efficiency in related technologies.
[0006] To achieve the above objectives, the technical solution adopted by this utility model is as follows: In a first aspect, an enrichment device for exosomes or viruses is provided, comprising: Housing unit; Two mounting units are removably disposed inside the housing unit; A plurality of first interception units are distributed between the two mounting units and located inside the outer casing unit for the first interception of particles in the liquid sample; A plurality of second interception units are distributed between the two mounting units and alternately arranged with a plurality of first interception units, and are located inside the outer shell unit for second interception of particles in the liquid sample; An inlet unit is removably disposed on one side of the outer casing unit and communicates with a plurality of first interception units and a plurality of second interception units respectively, for allowing liquid samples to enter the plurality of first interception units and a plurality of second interception units; A flushing and guiding unit is disposed at the top of the inlet unit and is connected to the inlet unit and the flushing pump. It is used to deliver flushing liquid to the inlet unit under the action of the flushing pump to flush the particles intercepted by the first interception unit and the second interception unit. A recovery and diversion unit is removably disposed on the other side of the outer shell unit and is connected to a plurality of first interception units, a plurality of second interception units, and a suction device, respectively, for sucking out the rinsed particles under the action of the suction device; An outlet unit is disposed at the bottom end of the outer shell unit and communicates with the outer shell unit, for discharging liquid samples that have been processed by a plurality of first retention units and a plurality of second retention units.
[0007] In some embodiments, the housing unit includes: The outer casing element has a plurality of first interception units and a plurality of second interception units disposed inside it. The inlet unit is disposed on one side of the outer casing element, and the recycling guide unit is disposed on the other side of the outer casing element. The outlet unit is disposed at the bottom end of the outer casing element. A first mounting element is disposed inside the housing element and is used to mount the two mounting units; The first through-slot element is disposed at the bottom end of the housing element and is connected to the first mounting element and the outlet unit respectively.
[0008] In some embodiments, the mounting unit includes: A second mounting element is disposed inside the housing unit; A plurality of second through-slot elements are respectively disposed through the second mounting element for the first intercepting unit to pass through; A plurality of third through slot elements are respectively disposed through the second mounting element and alternately disposed with the plurality of second through slot elements for the second intercepting unit to pass through.
[0009] In some embodiments, the first interception unit includes: A first interception element is disposed between the two mounting units and alternately disposed with the second interception unit, and is located inside the outer casing unit, for the first interception of particles in the liquid sample.
[0010] In some embodiments, the second interception unit includes: The second interception element is disposed between the two mounting units and alternately disposed with the first interception unit, and is located inside the outer casing unit, for second interception of particles in the liquid sample.
[0011] In some embodiments, the inlet unit includes: An inlet element is removably disposed on one side of the housing unit, and the flushing guide unit is disposed at the top of the inlet element; A first channel element is disposed through the inlet element and is connected to a plurality of first interception units and a plurality of second interception units respectively, for allowing liquid samples to enter the plurality of first interception units and the plurality of second interception units; The second channel element is disposed at the top of the inlet element and is connected to the first channel element and the flushing guide unit respectively.
[0012] In some embodiments, the flushing and guiding unit includes: A flushing guide element is disposed at the top of the inlet unit and is connected to the inlet unit and the flushing pump. It is used to deliver flushing liquid to the inlet unit under the action of the flushing pump to flush the particles trapped by the first interception unit and the second interception unit.
[0013] In some embodiments, the recycling diversion unit includes: A recyclable flow guiding element is removably disposed on the other side of the housing unit; The third channel element is disposed through the recycling guide element and is connected to several first interception units, several second interception units, and a suction device, respectively, for sucking out the rinsed particles under the action of the suction device.
[0014] In some embodiments, the outlet unit includes: An outlet element is disposed at the bottom end of the housing unit and communicates with the housing unit for discharging liquid samples that have been processed by a plurality of first retention units and a plurality of second retention units.
[0015] Secondly, an enrichment system is provided, comprising: The enrichment device as described in the first aspect; A rinsing device, which is connected to the rinsing guide unit of the enrichment device, is used to transport rinsing fluid to the rinsing guide unit; A suction device, which is connected to the recovery guide unit of the enrichment device, is used to suck out the target particles after rinsing. A first fluid control device is disposed inside the inlet unit of the enrichment device and is used to open and close the inlet unit; A second fluid control device is disposed inside the flushing guide unit of the enrichment device and is used to open and close the flushing guide unit. A third fluid control device is disposed inside the recovery guide unit of the enrichment device and is used to open and close the recovery guide unit. A fourth fluid control device is disposed inside the outlet unit of the enrichment device and is used to open and close the outlet unit.
[0016] The present invention adopts the above technical solution and has the following technical effects compared with the prior art: This invention discloses an enrichment device and system for exosomes or viruses. The device utilizes the cooperation between the outer shell unit and the mounting unit to provide positioning support for the mounting unit, ensuring a stable flow path for the liquid sample and preventing leakage or flow deviation during sample transport, thus guaranteeing the continuity of the enrichment process. The cooperation between the first and second retention units achieves particle retention; through a design adapted to particle size, it ensures that particles are fully retained as the liquid sample flows through, while allowing small molecule waste liquid to pass through. The flushing and guiding unit provides the flushing effect of the first and second retention units, enabling directional delivery of the collected liquid and stripping away retained particles, preventing saturation or clogging caused by particle adhesion, and solving the problems of traditional devices being unable to flush online and prone to membrane module clogging. The recovery and guiding unit provides particle recovery; through negative pressure suction, the flushed particle collection liquid is directly drawn out, avoiding secondary contamination. Attached Figure Description
[0017] Figure 1 This is a three-dimensional structural schematic diagram of the enrichment device according to an embodiment of the present utility model; Figure 2This is an exploded view of the enrichment device according to an embodiment of the present utility model; Figure 3 This is a cross-sectional view of the enrichment device according to an embodiment of the present utility model; Figure 4 This is a partial enlarged view of the enrichment device according to an embodiment of the present utility model; Figure 5 This is a cross-sectional view of the outer shell unit according to an embodiment of the present utility model; Figure 6 This is a partial enlarged view of the mounting unit according to an embodiment of the present utility model; Figure 7 This is a partial structural schematic diagram of the first interception unit according to an embodiment of the present utility model; Figure 8 This is a partial structural schematic diagram of the second interception unit according to an embodiment of the present utility model; Figure 9 This is a cross-sectional view of the inlet unit according to an embodiment of the present utility model; Figure 10 This is a three-dimensional structural schematic diagram of the flushing and guiding unit according to an embodiment of the present utility model; Figure 11 This is a cross-sectional view of the recycling and diversion unit according to an embodiment of the present utility model; Figure 12 This is a three-dimensional structural schematic diagram of the outlet unit according to an embodiment of the present utility model; Figure 13 This is a schematic diagram of the enrichment system according to an embodiment of the present invention.
[0018] The reference numerals in the figures are: 100, enrichment device; 110. Housing unit; 111. Housing element; 112. First mounting element; 113. First through-slot element; 120. Mounting unit; 121. Second mounting element; 122. Second through-slot element; 123. Third through-slot element; 130. First interception unit; 131. First interception element; 140. Second interception unit; 141. Second interception element; 150. Entry unit; 151. Entry element; 152. First channel element; 153. Second channel element; 160. Flushing guide unit; 161. Flushing guide element; 170. Recovery and diversion unit; 171. Recovery and diversion element; 172. Third channel element; 180. Export unit; 181. Export component; 200, Flushing device; 300, Suction device; 400, First fluid control device; 500, Second fluid control device; 600, Third fluid control device; 700, Fourth fluid control device. Detailed Implementation
[0019] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.
[0020] It should be noted that, unless otherwise specified, the embodiments and features described in these embodiments can be combined with each other.
[0021] The present invention will be further described below with reference to the accompanying drawings and specific embodiments, but this is not intended to limit the present invention.
[0022] Example 1 This embodiment relates to the enrichment device of this utility model.
[0023] like Figure 1 , Figure 2 , Figure 3 , Figure 4As shown, an enrichment device 100 for exosomes or viruses includes a housing unit 110, two mounting units 120, a plurality of first retention units 130, a plurality of second retention units 140, an inlet unit 150, a flushing and guiding unit 160, a recovery and guiding unit 170, and an outlet unit 180. The two mounting units 120 are removably disposed inside the housing unit 110; the plurality of first retention units 130 are distributed between the two mounting units 120 and located inside the housing unit 110, for the first retention of particles in the liquid sample; the plurality of second retention units 140 are distributed between the two mounting units 120, alternating with the plurality of first retention units 130, and located inside the housing unit 110, for the second retention of particles in the liquid sample; the inlet unit 150 is removably disposed on one side of the housing unit 110 and communicates with the plurality of first retention units 130 and the plurality of second retention units 140, for allowing the liquid sample to enter the plurality of first retention units 130 and the plurality of second retention units 140. 40; The flushing guide unit 160 is disposed at the top of the inlet unit 150 and is connected to the inlet unit 150 and the flushing pump. It is used to deliver flushing liquid to the inlet unit 150 under the action of the flushing pump to flush the particles intercepted by the first interception unit 130 and the second interception unit 140; The recovery guide unit 170 is removably disposed on the other side of the outer shell unit 110 and is connected to several first interception units 130, several second interception units 140 and the aspirator respectively. It is used to suck out the flushed particles under the action of the aspirator; The outlet unit 180 is disposed at the bottom of the outer shell unit 110 and is connected to the outer shell unit 110. It is used to discharge the liquid sample after being processed by several first interception units 130 and several second interception units 140.
[0024] In some embodiments, the plurality of first interception units 130 may be divided into several groups. Specifically, each group is provided with a plurality of first interception units 130, and the plurality of first interception units 130 in each group are equally spaced along the circumference of the mounting unit 120.
[0025] In some embodiments, the plurality of second interception units 140 may be divided into several groups. Specifically, each group is provided with a plurality of second interception units 140, and the plurality of second interception units 140 in each group are equally spaced along the circumference of the mounting unit 120.
[0026] In some embodiments, groups of several first interception units 130 and groups of several second interception units 140 are alternately arranged. Specifically, they are alternately arranged as follows: a first group of several first interception units 130, a first group of several second interception units 140, a second group of several first interception units 130, a second group of several second interception units 140... like Figure 5As shown, the outer casing unit 110 includes an outer casing element 111, a first mounting element 112, and a first through-slot element 113. The outer casing element 111 has several first interception units 130 and several second interception units 140 inside. An inlet unit 150 is located on one side of the outer casing element 111, and a recovery and diversion unit 170 is located on the other side. An outlet unit 180 is located at the bottom of the outer casing element 111. The first mounting element 112 is located inside the outer casing element 111 and is used to mount two mounting units 120. The first through-slot element 113 is located at the bottom of the outer casing element 111 and communicates with both the first mounting element 112 and the outlet unit 180.
[0027] The outer casing element 111 has a hollow structure.
[0028] In some of these embodiments, the housing element 111 is made of plastic.
[0029] In some of these embodiments, housing element 111 is a housing body.
[0030] The cross-section of the first mounting element 112 is circular.
[0031] The dimensions of the first mounting element 112 are matched with the dimensions of the housing element 111. Generally, the radial dimension (diameter) of the first mounting element 112 is smaller than the radial dimension (outer diameter) of the outer edge surface of the housing element 111, and the axial dimension of the first mounting element 112 is smaller than the axial dimension of the housing element 111.
[0032] In some of these embodiments, the first mounting element 112 is a mounting slot.
[0033] The cross-section of the first through-slot element 113 is circular.
[0034] The dimensions of the first through-slot element 113 are matched with the dimensions of the housing element 111. Generally, the radial dimension of the first through-slot element 113 is smaller than the radial dimension of the inner edge surface of the housing element 111, and the axial dimension of the first through-slot element 113 is equal to the shell wall thickness of the housing element 111.
[0035] In some of these embodiments, the first through slot element 113 is a first through hole.
[0036] like Figure 6As shown, the mounting unit 120 includes a second mounting element 121, a plurality of second through-slot elements 122, and a plurality of third through-slot elements 123. The second mounting element 121 is disposed inside the housing unit 110; the plurality of second through-slot elements 122 are respectively disposed through the second mounting element 121 for the first intercepting unit 130 to pass through; the plurality of third through-slot elements 123 are respectively disposed through the second mounting element 121 and are alternately disposed with the plurality of second through-slot elements 122 for the second intercepting unit 140 to pass through.
[0037] Specifically, the second mounting element 121 is disposed inside the first mounting element 112.
[0038] The cross-section of the second mounting element 121 is circular.
[0039] The dimensions of the second mounting element 121 are matched with the dimensions of the first mounting element 112. Generally, the radial dimension of the second mounting element 121 is equal to the radial dimension of the first mounting element 112, and the axial dimension of the second mounting element 121 is smaller than the axial dimension of the first mounting element 112.
[0040] In some of these embodiments, the second mounting element 121 is made of plastic.
[0041] In some embodiments, the second mounting element 121 is a mounting plate. A sealing ring (not shown) may be provided on the outer edge of the second mounting element 121 to increase the sealing between the second mounting element 121 and the first mounting element 112.
[0042] The cross-section of the second through-slot element 122 is circular.
[0043] The dimensions of the second through-slot element 122 are matched with the dimensions of the second mounting element 121. Generally, the radial dimension of the second through-slot element 122 is smaller than the radial dimension of the second mounting element 121, and the axial dimension of the second through-slot element 122 is equal to the axial dimension of the second mounting element 121.
[0044] The number of second through-slot elements 122 matches the number of first retaining units 130. Generally, the number of second through-slot elements 122 is equal to the number of first retaining units 130.
[0045] In some of these embodiments, the second through slot element 122 is a second through hole.
[0046] The cross-section of the third through-slot element 123 is circular.
[0047] The dimensions of the third through-slot element 123 are matched with the dimensions of the second mounting element 121. Generally, the radial dimension of the third through-slot element 123 is smaller than the radial dimension of the second mounting element 121, and the axial dimension of the third through-slot element 123 is equal to the axial dimension of the second mounting element 121.
[0048] The number of third through-slot elements 123 matches the number of second interception units 140. Generally, the number of third through-slot elements 123 is equal to the number of second interception units 140.
[0049] In some of these embodiments, the third through slot element 123 is a third through hole.
[0050] like Figure 7 As shown, the first interception unit 130 includes a first interception element 131. The first interception element 131 is disposed between the two mounting units 120 and alternately disposed with the second interception unit 140, and is located inside the housing unit 110, for the first interception of particles in the liquid sample.
[0051] Specifically, the first intercepting element 131 is inserted into the corresponding second through slot element 122 and is located inside the housing element 111.
[0052] The first intercepting element 131 has a hollow structure.
[0053] The dimensions of the first retaining element 131 are matched with the dimensions of the second through-slot element 122. Generally, the radial dimension of the outer edge of the first retaining element 131 is equal to the radial dimension of the second through-slot element 122, and the axial dimension of the first retaining element 131 is greater than the axial dimension of the second through-slot element 122.
[0054] The dimensions of the first retaining element 131 are matched with the dimensions of the housing element 111. Generally, the axial dimension of the first retaining element 131 is equal to the axial dimension of the housing element 111.
[0055] In some embodiments, the first retention element 131 is a first fiber filter. The first fiber filter of the first retention element 131 is made of hollow fibers. The pore size of the first fiber filter is approximately 200-1000 nm.
[0056] In some embodiments, the first intercepting element 131 is inserted into the corresponding second through-slot element 122, wherein the epoxy resin can be impregnated to achieve a curing and sealing effect.
[0057] like Figure 8As shown, the second interception unit 140 includes a second interception element 141. The second interception element 141 is disposed between the two mounting units 120 and alternately disposed with the first interception unit 130, and is located inside the housing unit 110, for second interception of particles in the liquid sample.
[0058] Specifically, the second intercepting element 141 is inserted into the corresponding third through slot element 123 and is alternately arranged with the first intercepting element 131, and is located inside the housing element 111.
[0059] The second intercepting element 141 has a hollow structure.
[0060] The dimensions of the second retaining element 141 are matched with the dimensions of the third through-slot element 123. Generally, the radial dimension of the outer edge of the second retaining element 141 is equal to the radial dimension of the third through-slot element 123, and the axial dimension of the second retaining element 141 is greater than the axial dimension of the third through-slot element 123.
[0061] The dimensions of the second tethering element 141 are matched with the dimensions of the first tethering element 131. Generally, the axial dimension of the second tethering element 141 is equal to the axial dimension of the first tethering element 131.
[0062] The dimensions of the second retaining element 141 are matched with the dimensions of the housing element 111. Generally, the axial dimension of the second retaining element 141 is equal to the axial dimension of the housing element 111.
[0063] In some embodiments, the second retention element 141 is a second fiber filter. The second fiber filter of the second retention element 141 is made of hollow fibers. The membrane pore size of the second fiber filter is approximately 20-100 nm.
[0064] In some embodiments, the second intercepting element 141 is inserted into the corresponding third through-slot element 123, wherein the epoxy resin can be impregnated to achieve a curing and sealing effect.
[0065] like Figure 9 As shown, the inlet unit 150 includes an inlet element 151, a first channel element 152, and a second channel element 153. The inlet element 151 is removably disposed on one side of the housing unit 110, and a flushing guide unit 160 is disposed at its top. The first channel element 152 passes through the inlet element 151 and communicates with several first interception units 130 and several second interception units 140, respectively, for allowing liquid samples to enter the several first interception units 130 and several second interception units 140. The second channel element 153 is disposed at the top of the inlet element 151 and communicates with both the first channel element 152 and the flushing guide unit 160.
[0066] Specifically, the inlet element 151 is removably disposed on one side of the housing element 111; the first channel element 152 is connected to the first intercepting element 131 and the second intercepting element 141 respectively.
[0067] In some embodiments, the inlet element 151 includes a first inlet pipe and a second inlet pipe. The first inlet pipe is removably disposed on one side of the housing element 111, and a second channel element 153 is disposed at the top end of the first inlet pipe, through which the first channel element 152 is disposed; the second inlet pipe is disposed on the side of the first inlet pipe, through which the first channel element 152 is disposed.
[0068] The dimensions of the first inlet pipe are matched with the dimensions of the housing element 111. Generally, the radial dimension of the first inlet pipe is greater than the radial dimension (outer diameter) of the outer edge of the housing element 111, and the axial dimension of the first inlet pipe is smaller than the axial dimension of the housing element 111.
[0069] The dimensions of the second inlet pipe are matched with those of the first inlet pipe. Generally, the radial dimension of the second inlet pipe is smaller than that of the first inlet pipe, and the axial dimension of the second inlet pipe is smaller than that of the first inlet pipe.
[0070] In some embodiments, the inlet element 151 is threadedly connected to the housing element 111, including but not limited to threaded connections.
[0071] In some of these embodiments, the inlet element 151 is made of plastic.
[0072] In some embodiments, the first channel element 152 includes a first channel, a second channel, and a third channel. The first channel extends through the second inlet element 151; the second channel is located on one side of the first inlet pipe and communicates with both the first channel and the second channel element 153; the third channel is located on the other side of the first inlet pipe and communicates with the second channel, the first interception element 131, and the second interception element 141.
[0073] In some of these embodiments, the first channel has a circular cross-section.
[0074] In some embodiments, the second channel has a tapered structure. Specifically, the radial dimension of the second channel increases from one side (the side closer to the first channel) to the other side (the side farther from the first channel).
[0075] In some of these embodiments, the third channel has a circular cross-section.
[0076] The dimensions of the first channel are matched with the dimensions of the inlet element 151. Generally, the radial dimension of the first channel is smaller than the radial dimension of the second inlet pipe, and the axial dimension of the first channel is equal to the axial dimension of the second inlet pipe.
[0077] The dimensions of the second channel are matched with the dimensions of the inlet element 151. Generally, the maximum radial dimension of the second channel is smaller than the radial dimension of the first inlet pipe, and the axial dimension of the second channel is smaller than the axial dimension of the first inlet pipe.
[0078] The dimensions of the second channel are matched with those of the first channel. Generally, the minimum radial dimension of the second channel is equal to the radial dimension of the first channel, and the axial dimension of the second channel is greater than that of the first channel.
[0079] The dimensions of the second channel are matched with the dimensions of the housing element 111. Generally, the maximum radial dimension of the second channel is equal to the radial dimension of the inner edge surface of the housing element 111.
[0080] The dimensions of the third channel are matched with the dimensions of the inlet element 151. Generally, the radial dimension of the third channel is smaller than the radial dimension of the first inlet pipe, and the axial dimension of the third channel is smaller than the axial dimension of the first inlet pipe.
[0081] The dimensions of the third channel are matched with those of the second channel. Generally, the radial dimension of the third channel is greater than the maximum radial dimension of the second channel, and the axial dimension of the third channel is smaller than the axial dimension of the second channel.
[0082] The dimensions of the third channel are matched with the dimensions of the housing element 111. Generally, the radial dimension of the third channel is equal to the radial dimension of the outer edge of the housing element 111.
[0083] The sum of the axial dimensions of the first channel, the second channel, and the third channel is equal to the sum of the axial dimensions of the first inlet pipe and the second inlet pipe.
[0084] The cross-section of the second channel element 153 is circular.
[0085] The dimensions of the second channel element 153 are matched with the dimensions of the inlet element 151. Generally, the radial dimension of the second channel element 153 is smaller than the radial dimension and axial dimension of the first inlet pipe, and the axial dimension of the second channel element 153 is smaller than the axial dimension of the first inlet pipe.
[0086] The dimensions of the second channel element 153 are matched with those of the first channel element 152. Generally, the radial dimension of the second channel element 153 is smaller than the minimum radial dimension of the second channel.
[0087] In some of these embodiments, the second channel element 153 is a fourth channel.
[0088] like Figure 10 The flushing guide unit 160 includes a flushing guide element 161. The flushing guide element 161 is disposed at the top of the inlet unit 150 and is connected to the inlet unit 150 and the flushing pump. It is used to deliver flushing liquid to the inlet unit 150 under the action of the flushing pump to flush the particles retained by the first interception unit 130 and the second interception unit 140.
[0089] Specifically, the flushing guide element 161 is disposed at the top of the inlet element 151 and is connected to the second channel element 153.
[0090] More specifically, the flushing guide element 161 is disposed at the top of the first inlet pipe and connected to the first inlet pipe.
[0091] The flushing guide element 161 has a hollow structure.
[0092] The dimensions of the flushing guide element 161 are matched with the dimensions of the inlet element 151. Generally, the radial dimension (outer diameter) of the outer edge of the flushing guide element 161 is smaller than the radial dimension and axial dimension of the first inlet pipe.
[0093] The dimensions of the flushing guide element 161 are matched with the dimensions of the second channel element 153. Generally, the radial dimension (inner diameter) of the inner edge surface of the flushing guide element 161 is equal to the radial dimension of the second channel element 153.
[0094] In some embodiments, the flushing guide element 161 is fixedly connected to the inlet element 151, including but not limited to integral molding.
[0095] In some of these embodiments, the flushing guide element 161 is made of plastic.
[0096] In some of these embodiments, the flushing guide element 161 is a flushing guide pipe.
[0097] like Figure 11 The recovery and diversion unit 170 includes a recovery and diversion element 171 and a third channel element 172. The recovery and diversion element 171 is removably disposed on the other side of the housing unit 110; the third channel element 172 is disposed through the recovery and diversion element 171 and is connected to a plurality of first interception units 130, a plurality of second interception units 140 and a suction device, respectively, for sucking out the rinsed particles under the action of the suction device.
[0098] Specifically, the recovery guide element 171 is removably disposed on the other side of the housing element 111; the third channel element 172 is connected to the first interception element 131 and the second interception element 141 respectively.
[0099] In some embodiments, the recycling guide element 171 includes a first recycling pipe and a second recycling pipe. The first recycling pipe is removably disposed on the other side of the housing element 111, and a third channel element 172 is disposed through the first recycling pipe; the second recycling pipe is disposed on the side of the first recycling pipe, and the third channel element 172 is disposed through the second recycling pipe.
[0100] The dimensions of the first recovery conduit are matched with the dimensions of the housing element 111. Generally, the radial dimension of the first recovery conduit is greater than the radial dimension (outer diameter) of the outer edge of the housing element 111, and the axial dimension of the first recovery conduit is smaller than the axial dimension of the housing element 111.
[0101] The dimensions of the second recovery pipe are matched with those of the first recovery pipe. Generally, the radial dimension of the second recovery pipe is smaller than that of the first recovery pipe, and the axial dimension of the second recovery pipe is smaller than that of the first recovery pipe.
[0102] In some of these embodiments, the recirculation guide element 171 is threadedly connected to the housing element 111, including but not limited to threaded connections.
[0103] In some of these embodiments, the recycling flow guide element 171 is made of plastic.
[0104] In some embodiments, the third channel element 172 includes a fifth channel, a sixth channel, and a seventh channel. The fifth channel extends through the second recycling guide element 171; the sixth channel is located on one side of the first recycling pipe and communicates with the fifth channel; the seventh channel is located on the other side of the first recycling pipe and communicates with the sixth channel, the first interception element 131, and the second interception element 141, respectively.
[0105] In some of these embodiments, the fifth channel has a circular cross-section.
[0106] In some embodiments, the sixth channel has a tapered structure. Specifically, the radial dimension of the sixth channel increases from one side (the side closer to the fifth channel) to the other side (the side farther from the fifth channel).
[0107] In some of these embodiments, the seventh channel has a circular cross-section.
[0108] The dimensions of the fifth channel are matched with the dimensions of the recovery guide element 171. Generally, the radial dimension of the fifth channel is smaller than the radial dimension of the second recovery pipe, and the axial dimension of the fifth channel is equal to the axial dimension of the second recovery pipe.
[0109] The dimensions of the sixth channel are matched with the dimensions of the recovery guide element 171. Generally, the maximum radial dimension of the sixth channel is smaller than the radial dimension of the first recovery pipe, and the axial dimension of the sixth channel is smaller than the axial dimension of the first recovery pipe.
[0110] The dimensions of the sixth channel are matched with those of the fifth channel. Generally, the minimum radial dimension of the sixth channel is equal to the radial dimension of the fifth channel, and the axial dimension of the sixth channel is greater than that of the fifth channel.
[0111] The dimensions of the sixth channel are matched with the dimensions of the housing element 111. Generally, the maximum radial dimension of the sixth channel is equal to the radial dimension of the inner edge surface of the housing element 111.
[0112] The dimensions of the seventh channel are matched with the dimensions of the recovery guide element 171. Generally, the radial dimension of the seventh channel is smaller than the radial dimension of the first recovery pipe, and the axial dimension of the seventh channel is smaller than the axial dimension of the first recovery pipe.
[0113] The dimensions of the seventh channel are matched to those of the sixth channel. Generally, the radial dimension of the seventh channel is greater than the maximum radial dimension of the sixth channel, and the axial dimension of the seventh channel is smaller than the axial dimension of the sixth channel.
[0114] The dimensions of the seventh channel are matched with the dimensions of the housing element 111. Generally, the radial dimension of the seventh channel is equal to the radial dimension of the outer edge of the housing element 111.
[0115] The sum of the axial dimensions of the fifth channel, the sixth channel, and the seventh channel is equal to the sum of the axial dimensions of the first recovery pipe and the second recovery pipe.
[0116] like Figure 12 As shown, the outlet unit 180 includes an outlet element 181. The outlet element 181 is disposed at the bottom end of the housing unit 110 and communicates with the housing unit 110, and is used to discharge the liquid sample after being processed by a plurality of first interception units 130 and a plurality of second interception units 140.
[0117] Specifically, the outlet element 181 is located at the bottom end of the housing element 111 and communicates with the first through slot element 113.
[0118] The export component 181 has a hollow structure.
[0119] The dimensions of the outlet element 181 are matched with the dimensions of the housing element 111. Generally, the radial dimension (outer diameter) of the outer edge of the outlet element 181 is smaller than the radial dimension and axial dimension of the outer edge of the housing element 111.
[0120] The dimensions of the outlet element 181 are matched with the dimensions of the first through-slot element 113. Generally, the radial dimension (inner diameter) of the inner edge surface of the outlet element 181 is equal to the radial dimension of the first through-slot element 113.
[0121] In some of these embodiments, the outlet element 181 is fixedly connected to the housing element 111, including but not limited to being integrally formed.
[0122] In some of these embodiments, the outlet element 181 is made of plastic.
[0123] In some of these embodiments, the outlet element 181 is an outlet pipe.
[0124] The method of using this utility model is as follows: (a) Preparation Place housing element 111 in the designated position. That is, position housing element 111 vertically (refer to the attached diagram for the vertical position). Figure 1 (as shown) is placed at the mounting bracket (not shown in the figure) and fixed in place by clamps; The first intercepting element 131 and the second intercepting element 141 are inserted into the first mounting element 112 through the two second mounting elements 121; Connect the inlet element 151 to one side (top) of the housing element 111 by thread until tightened; Finally, the inlet element 151 is brought into contact with the side of the corresponding second mounting element 121, so that the first channel element 152 is connected to the first intercepting element 131 and the second intercepting element 141. Connect the recycling guide element 171 to the other side (bottom end) of the housing element 111 by thread until it is tightened; Finally, the recycling guide element 171 is brought into contact with the other side of the housing element 111, so that the third channel element 172 is connected to the first interception element 131 and the second interception element 141. Connect the flushing pump to the flushing guide element 161 via a connecting pipe; The suction device is connected to the recovery guide element 171 via a connecting pipe.
[0125] (ii) Processing Operations The liquid sample enters the first channel element 152 through the inlet element 151, and then enters the first retention element 131 and the second retention element 141 through the first channel element 152. The particles (exosomes or virus particles) in the liquid sample are filtered and retained by the first retention element 131 and the second retention element 141 (that is, the particles are retained in the cavity of the first retention element 131 and the second retention element 141). Finally, the processed liquid sample is discharged through the first channel element 113 and the outlet element 181 (it can be collected using a waste liquid collection tank).
[0126] (III) Pellet Recovery The flushing pump is started to deliver the collected liquid to the flushing guide element 161, and then from the flushing guide element 161 to the second channel element 153 and the first channel element 152, thereby flushing the trapped particles and removing them from the cavity of the first interception element 131 and the second interception element 141 (i.e., the particles are in the third channel element 172). At this time, the particles and the collected liquid are mixed to form a particle concentrate. During the process, the suction device is activated to generate negative pressure, and the concentrated particulate liquid is extracted and recovered by the recovery guide element 171.
[0127] (iv) Changing operations Twist the inlet element 151 until it is removed from one side of the housing element 111; The first retaining element 131 and the second retaining element 141 that need to be replaced are removed from the first mounting element 112 through the two second mounting elements 121; The new first intercepting element 131 and the second intercepting element 141 are inserted into the first mounting element 112 through the two second mounting elements 121; Connect the inlet element 151 to one side of the housing element 111 by thread until tightened; Finally, the inlet element 151 is brought into contact with the side of the corresponding second mounting element 121, so that the first channel element 152 is connected to the new first intercepting element 131 and the new second intercepting element 141.
[0128] The advantages of this invention are as follows: The cooperation between the outer shell unit and the mounting unit provides positioning support for the mounting unit, ensuring a stable flow path for the liquid sample and preventing leakage or flow deviation during sample transport, thus guaranteeing the continuity of the enrichment process. The cooperation between the first and second interception units achieves particle interception; the design adapts to particle size, ensuring sufficient particle interception as the liquid sample flows through, while allowing small molecule waste liquid to pass through. The rinsing and guiding unit provides the rinsing effect of the first and second interception units, enabling directional delivery of the collected liquid and stripping away intercepted particles, preventing saturation or blockage caused by particle adhesion, and solving the problems of traditional devices being unable to perform online rinsing and membrane module clogging. The recovery and guiding unit provides particle recovery; the rinsed particle collection liquid is directly sucked out using negative pressure suction, avoiding secondary pollution.
[0129] Example 2 This embodiment relates to the enrichment system of this utility model.
[0130] like Figure 13 As shown, an enrichment system includes an enrichment device 100, a rinsing device 200, a suction device 300, a first fluid control device 400, a second fluid control device 500, a third fluid control device 600, and a fourth fluid control device 700 as described in Example 1. The flushing device 200 is connected to the flushing guide unit 160 of the enrichment device 100 and is used to transport the flushing liquid to the flushing guide unit 160; the suction device 300 is connected to the recovery guide unit 170 of the enrichment device 100 and is used to suck out the target particles after flushing; the first fluid control device 400 is disposed inside the inlet unit 150 of the enrichment device 100 and is used to open and close the inlet unit 150; the second fluid control device 500 is disposed inside the flushing guide unit 160 of the enrichment device 100 and is used to open and close the flushing guide unit 160; the third fluid control device 600 is disposed inside the recovery guide unit 170 of the enrichment device 100 and is used to open and close the recovery guide unit 170; the fourth fluid control device 700 is disposed inside the outlet unit 180 of the enrichment device 100 and is used to open and close the outlet unit 180.
[0131] Specifically, the flushing device 200 is connected to the flushing guide element 161; the suction device 300 is connected to the recovery guide element 171; the first fluid control device 400 is disposed inside the first channel element 152; the second fluid control device 500 is disposed inside the flushing guide element 161; the third fluid control device 600 is disposed inside the third channel element 172; and the fourth fluid control device 700 is disposed inside the outlet element 181.
[0132] More specifically, the first fluid control device 400 is disposed inside the first channel; the third fluid control device 600 is disposed inside the fifth channel; In some of these embodiments, the flushing device 200 is a flushing pump.
[0133] In some of these embodiments, the suction device 300 is a suction pump.
[0134] In some of these embodiments, the first fluid control device 400 is a first control valve.
[0135] In some of these embodiments, the second fluid control device 500 is a second control valve.
[0136] In some of these embodiments, the third fluid control device 600 is a third control valve.
[0137] In some of these embodiments, the fourth fluid control device 700 is a fourth control valve.
[0138] The method of using this utility model is as follows: (a) Preparation Place housing element 111 in the designated position. That is, position housing element 111 vertically (refer to the attached diagram for the vertical position). Figure 1 (as shown) is placed at the mounting bracket (not shown in the figure) and fixed in place by clamps; The first intercepting element 131 and the second intercepting element 141 are inserted into the first mounting element 112 through the two second mounting elements 121; Connect the inlet element 151 to one side (top) of the housing element 111 by thread until tightened; Finally, the inlet element 151 is brought into contact with the side of the corresponding second mounting element 121, so that the first channel element 152 is connected to the first intercepting element 131 and the second intercepting element 141. Connect the recycling guide element 171 to the other side (bottom end) of the housing element 111 by thread until it is tightened; Finally, the recycling guide element 171 is brought into contact with the other side of the housing element 111, so that the third channel element 172 is connected to the first interception element 131 and the second interception element 141. The flushing device 200 is connected to the flushing guide element 161 via a connecting pipe; The suction device 300 is connected to the recovery guide element 171 via a connecting pipe.
[0139] (ii) Processing Operations The first fluid control device 400 is controlled to operate, causing it to open the first channel element 152; The second fluid control device 500 is controlled to operate, causing it to close and flush the guide element 161. The third fluid control device 600 is controlled to operate, causing it to close the third channel element 172; The fourth fluid control device 700 is controlled to operate, causing it to open the outlet element 181; The liquid sample enters the first channel element 152 through the inlet element 151, and then enters the first retention element 131 and the second retention element 141 through the first channel element 152. The particles (exosomes or virus particles) in the liquid sample are filtered and retained by the first retention element 131 and the second retention element 141 (that is, the particles are retained in the cavity of the first retention element 131 and the second retention element 141). Finally, the processed liquid sample is discharged through the first channel element 113 and the outlet element 181 (it can be collected using a waste liquid collection tank).
[0140] (III) Pellet Recovery The first fluid control device 400 is controlled to operate, causing it to close the first channel element 152; The second fluid control device 500 is controlled to operate, causing it to open the flushing guide element 161; The third fluid control device 600 is controlled to open the third channel element 172. At this time, the liquid sample accumulated in the third channel element 172 is completely drained (it can be collected using a waste liquid collection box). The fourth fluid control device 700 is controlled to operate, causing it to close the outlet element 181; The flushing pump is started to deliver the collected liquid to the flushing guide element 161, and then from the flushing guide element 161 to the second channel element 153 and the first channel element 152, thereby flushing the trapped particles and removing them from the cavity of the first interception element 131 and the second interception element 141 (i.e., the particles are in the third channel element 172). At this time, the particles and the collected liquid are mixed to form a particle concentrate. During the process, the suction device is activated to generate negative pressure, and the concentrated particulate liquid is extracted and recovered by the recovery guide element 171.
[0141] (iv) Changing operations The usage method is basically the same as that in Example 1 (IV), and will not be repeated here.
[0142] The above description is only a preferred embodiment of the present utility model and does not limit the implementation method and protection scope of the present utility model. Those skilled in the art should realize that all solutions obtained by equivalent substitutions and obvious changes made based on the description and illustrations of the present utility model should be included within the protection scope of the present utility model.
Claims
1. An enrichment device for exosomes or viruses, characterized in that, include: Housing unit; Two mounting units are removably disposed inside the housing unit; A plurality of first interception units are distributed between the two mounting units and located inside the outer casing unit for the first interception of particles in the liquid sample; A plurality of second interception units are distributed between the two mounting units and alternately arranged with a plurality of first interception units, and are located inside the outer shell unit for second interception of particles in the liquid sample; An inlet unit is removably disposed on one side of the outer casing unit and communicates with a plurality of first interception units and a plurality of second interception units respectively, for allowing liquid samples to enter the plurality of first interception units and a plurality of second interception units; A flushing and guiding unit is disposed at the top of the inlet unit and is connected to the inlet unit and the flushing pump. It is used to deliver flushing liquid to the inlet unit under the action of the flushing pump to flush the particles intercepted by the first interception unit and the second interception unit. A recovery and diversion unit is removably disposed on the other side of the outer shell unit and is connected to a plurality of first interception units, a plurality of second interception units, and a suction device, respectively, for sucking out the rinsed particles under the action of the suction device; An outlet unit is disposed at the bottom end of the outer shell unit and communicates with the outer shell unit, for discharging liquid samples that have been processed by a plurality of first retention units and a plurality of second retention units.
2. The enrichment device according to claim 1, characterized in that, The outer casing unit includes: The outer casing element has a plurality of first interception units and a plurality of second interception units disposed inside it. The inlet unit is disposed on one side of the outer casing element, and the recycling guide unit is disposed on the other side of the outer casing element. The outlet unit is disposed at the bottom end of the outer casing element. A first mounting element is disposed inside the housing element and is used to mount the two mounting units; The first through-slot element is disposed at the bottom end of the housing element and is connected to the first mounting element and the outlet unit respectively.
3. The enrichment device according to claim 1, characterized in that, The installation unit includes: A second mounting element is disposed inside the housing unit; A plurality of second through-slot elements are respectively disposed through the second mounting element for the first intercepting unit to pass through; A plurality of third through slot elements are respectively disposed through the second mounting element and alternately disposed with the plurality of second through slot elements for the second intercepting unit to pass through.
4. The enrichment device according to claim 1, characterized in that, The first interception unit includes: A first interception element is disposed between the two mounting units and alternately disposed with the second interception unit, and is located inside the outer casing unit, for the first interception of particles in the liquid sample.
5. The enrichment device according to claim 1, characterized in that, The second interception unit includes: The second interception element is disposed between the two mounting units and alternately disposed with the first interception unit, and is located inside the outer casing unit, for second interception of particles in the liquid sample.
6. The enrichment device according to claim 1, characterized in that, The inlet unit includes: An inlet element is removably disposed on one side of the housing unit, and the flushing guide unit is disposed at the top of the inlet element; A first channel element is disposed through the inlet element and is connected to a plurality of first interception units and a plurality of second interception units respectively, for allowing liquid samples to enter the plurality of first interception units and the plurality of second interception units; The second channel element is disposed at the top of the inlet element and is connected to the first channel element and the flushing guide unit, respectively.
7. The enrichment device according to claim 1, characterized in that, The flushing and guiding unit includes: A flushing guide element is disposed at the top of the inlet unit and is connected to the inlet unit and the flushing pump. It is used to deliver flushing liquid to the inlet unit under the action of the flushing pump to flush the particles trapped by the first interception unit and the second interception unit.
8. The enrichment device according to claim 1, characterized in that, The recycling diversion unit includes: A recyclable flow guiding element is removably disposed on the other side of the housing unit; The third channel element is disposed through the recycling guide element and is connected to several first interception units, several second interception units, and a suction device, respectively, for sucking out the rinsed particles under the action of the suction device.
9. The enrichment device according to claim 1, characterized in that, The outlet unit includes: An outlet element is disposed at the bottom end of the housing unit and communicates with the housing unit for discharging liquid samples that have been processed by a plurality of first retention units and a plurality of second retention units.
10. An enrichment system, characterized in that, include: The enrichment device as described in any one of claims 1 to 9; A rinsing device, which is connected to the rinsing guide unit of the enrichment device, is used to transport rinsing fluid to the rinsing guide unit; A suction device, which is connected to the recovery guide unit of the enrichment device, is used to suck out the target particles after rinsing. A first fluid control device is disposed inside the inlet unit of the enrichment device and is used to open and close the inlet unit; A second fluid control device is disposed inside the flushing guide unit of the enrichment device and is used to open and close the flushing guide unit. A third fluid control device is disposed inside the recovery guide unit of the enrichment device and is used to open and close the recovery guide unit. A fourth fluid control device is disposed inside the outlet unit of the enrichment device and is used to open and close the outlet unit.