A test card for progesterone

Abstract

The utility model discloses a kind of progesterone detection cards, including detection card body, the surface one side of detection card body is equipped with reaction area, and the surface other side of detection card body is equipped with sample adding hole, the surface of detection card body is also provided with sample adding and its filtering mechanism, and sample adding and its filtering mechanism are provided with sample groove, the surface of sample groove is covered with filter sheet, and the inside fixed setting of filter sheet has filter screen.The progesterone detection card, the filter screen on filter sheet can intercept red blood cell, leukocyte, platelet fragment, blood clot and other interfering impurities in urine;If these impurities are not filtered, easy to adhere to the detection area of detection card body, interfere the specificity of progesterone and detection reagent, lead to false positive, false negative or result deviation;And the filtering effect of filter screen can remove the above interference source, create pure detection environment for detection card body, directly improve the accuracy of progesterone detection result.

Description

Technical Field

[0001] This utility model relates to the field of biological detection, specifically a progesterone detection card. Background Technology

[0002] The progesterone test strip primarily operates based on colloidal gold immunochromatography technology. Its core principle is as follows: Sample reaction: The user drips urine or serum samples into the sample well. The sample, through capillary action, carries the colloidal gold-progesterone antibody complex on the conjugate pad forward. If the sample contains progesterone, it binds to the antibody in the complex, forming a new progesterone-colloidal gold antibody complex. This complex continues to flow to the detection line (T line) on the NC membrane, where it is captured by another type of progesterone antibody on the T line, aggregating to form a red band. Regardless of the presence of progesterone in the sample, the unbound colloidal gold-progesterone antibodies flow to the control line (C line), binding to substances on the C line and developing color, thus proving the test strip's effectiveness. The final test result is determined by whether the T line and C line develop color. Progesterone testing relies on the specific binding of progesterone molecules in the sample to antibodies on the test card (such as the double-antibody sandwich method). Impurities in the sample can directly enter the reaction area and disrupt the reaction environment: red blood cells, white blood cells, platelet fragments, or blood clots caused by insufficient centrifugation in blood samples; epithelial cells, salt crystals, and flocculent precipitates in urine samples. The diameter of these impurity particles may exceed the pore size (usually 1-10 μm) of the nitrocellulose membrane (NC membrane) on the test card, blocking the liquid flow channels on the membrane. This prevents the sample from penetrating evenly to the test line (T line) and control line (C line), resulting in invalid results such as "no band," "incomplete band," or "control line not developing color," or even a false judgment of "progesterone negative." If impurities bind to the detection antibody, they will occupy the antibody's specific binding site, preventing progesterone from binding normally and resulting in a false negative. Utility Model Content

[0003] The purpose of this invention is to provide a progesterone testing card to solve the problems mentioned in the background art.

[0004] To achieve the above objectives, a progesterone test card is provided, comprising a test card body, a reaction zone on one side of the surface of the test card body, and a sample application hole on the other side of the surface of the test card body. The surface of the test card body is also provided with a sample application and filtration mechanism, and a sample slot is provided on the sample application and filtration mechanism. The surface of the sample slot is covered with a filter sheet, and a filter screen is fixedly installed inside the filter sheet.

[0005] Furthermore, the detection card body also includes a splicing slot and a splicing base, and two sets of both the splicing slot and the splicing base are provided.

[0006] Furthermore, two sets of splicing slots are provided on one side of the surface of the detection card body, and two sets of splicing seats are fixedly provided on the other side of the surface of the detection card body, the dimensions of the splicing seats and the splicing slots being compatible.

[0007] Furthermore, the splicing seat is inserted into the splicing slot, and the splicing seat and the splicing slot are dovetail-shaped. Adjacent sets of the detection card bodies are assembled by connecting the splicing seat and the splicing slot.

[0008] Furthermore, the sample addition and filtration mechanism also includes a limiting groove, and the limiting groove is configured as two sets respectively opened on both sides of the sample groove surface.

[0009] Furthermore, the sample slot is rectangular, and the filter inside the sample slot is rectangular, with a circular filter screen in the middle of the filter screen covering directly above the sample application hole.

[0010] Furthermore, the diameter of the filter screen is larger than the diameter of the sample feeding hole. Both sides of the surface of the filter are fixed with a fixing seat, and a gas receiving plate is installed on one side of the top of the fixing seat. At the same time, a limiting block is installed on the other side of the top of the fixing seat. The size of the limiting block is adapted to the limiting groove, and the bottom of the limiting block is inserted into the limiting groove.

[0011] Compared with the prior art, the beneficial effects of this utility model are: 1. In this method, after urine is poured into the sample slot inside the sample application and filtration mechanism, the filter screen on the filter can intercept red blood cells, white blood cells, platelet fragments, blood clots, and other interfering impurities in the urine. If these impurities are not filtered, they can easily adhere to the detection area of ​​the test card, interfering with the specific reaction between progesterone and the test reagent, leading to false positives, false negatives, or result deviations. The filtration function of the filter screen can remove the above-mentioned interference sources, creating a pure detection environment for the test card and directly improving the accuracy of progesterone test results. 2. This solution achieves docking and assembly by connecting two adjacent sets of test cards through splicing slots and splicing bases. The modular splicing method allows for flexible adjustment of the number of test cards according to testing needs. When testing a single sample, a single test card can be used. When testing batch samples, multiple test cards can be assembled into a whole through multiple splicing slots and splicing bases. The assembled test card set can adapt to the synchronous sample addition and synchronous reading process of automated testing equipment, avoiding the tedious operation of each test card one by one, greatly improving the testing efficiency of batch samples, and reducing the space occupied when a single test card is placed independently, thus optimizing the layout of the testing operation table. Attached Figure Description

[0012] Figure 1 This is a front view schematic diagram of the structure of this utility model; Figure 2 This is a bottom view of the structure of this utility model; Figure 3 This is a structural diagram of the main body of the structural testing card of this utility model; Figure 4 This is a cross-sectional view of the structure detection card body of this utility model; Figure 5 This is a schematic diagram of the filter element and its connection structure according to the present invention.

[0013] The diagram is labeled as follows: 100, Test card body; 11, Splicing groove; 12, Splicing seat; 13, Reaction zone; 14, Sample addition and filtration mechanism; 141, Sample slot; 142, Sample addition hole; 143, Limiting groove; 144, Filter plate; 1441, Filter screen; 1442, Fixing seat; 1443, Gas receiving plate; 1444, Limiting block. Detailed Implementation

[0014] Please see Figure 1-5 This utility model provides a progesterone test card, including a test card body 100. A reaction zone 13 is provided on one side of the surface of the test card body 100, and a sample application hole 142 is provided on the other side of the surface of the test card body 100. A sample application and filtration mechanism 14 is also provided on the surface of the test card body 100, and a sample groove 141 is provided on the sample application and filtration mechanism 14. A filter sheet 144 is covered on the surface of the sample groove 141, and a filter screen 1441 is fixedly provided inside the filter sheet 144.

[0015] Working principle: Urine is poured into the sample slot 141 inside the sample loading and filtration mechanism 14. The filter screen 1441 on the filter element 144 filters out red blood cells, white blood cells, platelet fragments, blood clots, and other interfering impurities in the urine, improving the accuracy of progesterone testing on the test card body 100. After the urine is poured into the sample slot 141 inside the sample loading and filtration mechanism 14, the filter screen 1441 on the filter element 144 intercepts red blood cells, white blood cells, platelet fragments, blood clots, and other interfering impurities in the urine. If these impurities are not filtered, they can easily adhere to the detection area of ​​the test card body 100, interfering with the specific reaction between progesterone and the test reagent, leading to false positives, false negatives, or result deviations. The filtration effect of the filter screen 1441 removes these interfering sources, creating a pure detection environment for the test card body 100 and directly improving the accuracy of progesterone test results. The filter 1441 has a consistent filtration effect on impurities, ensuring that the degree of impurity removal remains stable for each urine sample poured into the sample tank 141, avoiding fluctuations in test results due to differences in impurity content. The stable pretreatment effect allows the test card body 100 to react under uniform sample purity conditions in progesterone testing of different batches and samples, improving the repeatability of test results and providing a reliable basis for subsequent data analysis and judgment.

[0016] In a preferred embodiment, the detection card body 100 also includes a splicing slot 11 and a splicing base 12, and two sets of both the splicing slot 11 and the splicing base 12 are provided.

[0017] Two sets of splicing slots 11 are provided on one side of the surface of the detection card body 100, and two sets of splicing seats 12 are fixedly provided on the other side of the surface of the detection card body 100. The dimensions of the splicing seats 12 and the splicing slots 11 are compatible.

[0018] The splicing base 12 is inserted into the splicing slot 11, and the splicing base 12 and the splicing slot 11 are in the shape of a dovetail. The adjacent two sets of test card bodies 100 are assembled by connecting and splicing through the splicing base 12 and the splicing slot 11.

[0019] like Figure 1-4 As shown: Two sets of splicing grooves 11 are provided on one side of the surface of the test card body 100, and two sets of splicing seats 12 are fixed on the other side. The dovetail design itself has the characteristics of preventing card detachment and offset, which can prevent adjacent test card bodies 100 from loosening in the horizontal or vertical direction after splicing. At the same time, the two sets of symmetrically distributed splicing grooves 11 and splicing seats 12 can evenly distribute the force at the splicing point, prevent damage to a single splicing structure due to concentrated force, further enhance the overall structural stability of adjacent test card bodies 100 after docking, and avoid sample loading or test reading due to splicing loosening during the test. Two adjacent sets of test card bodies 100 are connected and assembled by splicing base 12 and splicing slot 11. The modular splicing method can flexibly adjust the number of test cards according to the testing needs. When testing a single sample, a single test card body 100 can be used alone. When testing batch samples, multiple test card bodies 100 can be assembled into a whole by using multiple splicing slots 11 and splicing base 12. The assembled test card group can adapt to the synchronous sample addition and synchronous reading process of automated testing equipment, avoiding the cumbersome operation of each test card, greatly improving the testing efficiency of batch samples, and reducing the space occupation when a single test card is placed independently, thus optimizing the layout of the testing operation table.

[0020] In a preferred embodiment, the sample addition and filtration mechanism 14 also includes a limiting groove 143, and the limiting groove 143 is configured as two sets respectively opened on both sides of the surface of the sample groove 141.

[0021] The sample slot 141 is rectangular, and the filter plate 144 inside the sample slot 141 is rectangular. The filter screen 1441 in the middle of the filter plate 144 is circular, and the filter screen 1441 covers the top of the sample feeding hole 142.

[0022] The diameter of the filter screen 1441 is larger than the diameter of the sample inlet 142. The filter sheet 144 has a fixed seat 1442 on both sides of its surface. A gas receiving plate 1443 is installed on one side of the top of the fixed seat 1442. At the same time, a limiting block 1444 is installed on the other side of the top of the fixed seat 1442. The size of the limiting block 1444 is matched with that of the limiting groove 143, and the bottom of the limiting block 1444 is inserted into the limiting groove 143.

[0023] After the test card body 100 is used up, the filter 144 on the test card body 100 is a detachable structure and can be reused. After the filter 144 is taken out from the sample slot 141, the filter 144 is cleaned and disinfected. like Figure 4-5 As shown: The filter 144 on the test card body 100 is a detachable structure and supports reuse. Compared with disposable filter 144 which is discarded after a single use, it can significantly reduce the frequency of filter replacement and the amount of filter purchased. Users do not need to equip a new filter 144 for each test. They can simply take the used filter 144 out of the sample slot 141, clean and disinfect it, and then reassemble it for use. In the long run, it can greatly reduce the cost of consumables in the testing process, reduce the waste of resources caused by discarded filter 144, and improve the economy of the test card body 100. If residual impurities such as red blood cells and white blood cells from urine remain on the used filter 144, it will affect the filtration effect of the next test and thus interfere with the progesterone test results of the test card body 100. The cleaning and disinfection operation after removing it from the sample slot 141 can specifically remove impurities, microorganisms and possible residual reagents attached to the filter screen 1441 of the filter 144, restoring the filtration performance of the filter 144. The cleaning and disinfection steps before reuse can ensure that the filter 144 can maintain a stable impurity interception capacity for each test, avoiding filtration failure caused by filter contamination or blockage, and providing a reliable guarantee for the accuracy of subsequent progesterone tests of the test card body 100.

Claims

1. A progesterone detection card, comprising a detection card body (100), wherein a reaction zone (13) is formed on one side of the surface of the detection card body (100), and a sample application hole (142) is formed on the other side of the surface of the detection card body (100), characterized in that: The surface of the test card body (100) is also provided with a sample addition and filtration mechanism (14), and a sample slot (141) is provided on the sample addition and filtration mechanism (14). The surface of the sample slot (141) is covered with a filter sheet (144), and a filter screen (1441) is fixedly provided inside the filter sheet (144).

2. The progesterone detection card according to claim 1, characterized in that: The detection card body (100) also includes a splicing slot (11) and a splicing base (12), and both the splicing slot (11) and the splicing base (12) are provided in two sets.

3. The progesterone detection card according to claim 2, characterized in that: Two sets of splicing slots (11) are provided on one side of the surface of the detection card body (100), and two sets of splicing seats (12) are fixedly provided on the other side of the surface of the detection card body (100). The sizes of the splicing seats (12) and the splicing slots (11) are compatible.

4. The progesterone detection card according to claim 3, characterized in that: The splicing seat (12) is inserted into the splicing groove (11), and the splicing seat (12) and the splicing groove (11) are in the shape of a dovetail. The two adjacent sets of the detection card bodies (100) are connected and assembled through the splicing seat (12) and the splicing groove (11).

5. A progesterone detection card according to claim 1, characterized in that: The sample addition and filtration mechanism (14) also includes a limiting groove (143), and the limiting groove (143) is configured as two sets respectively opened on both sides of the surface of the sample groove (141).

6. A progesterone detection card according to claim 5, characterized in that: The sample slot (141) is rectangular, and the filter plate (144) inside the sample slot (141) is rectangular. The filter screen (1441) in the middle of the filter plate (144) is circular, and the filter screen (1441) covers the sample feeding hole (142) directly above it.

7. A progesterone detection card according to claim 6, characterized in that: The diameter of the filter screen (1441) is larger than the diameter of the sample inlet (142). The filter sheet (144) has a fixed seat (1442) on both sides of its surface. A gas receiving plate (1443) is installed on one side of the top of the fixed seat (1442). At the same time, a limiting block (1444) is installed on the other side of the top of the fixed seat (1442). The size of the limiting block (1444) is adapted to the limiting groove (143), and the bottom of the limiting block (1444) is inserted into the limiting groove (143).

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