Fluid device, method for its manufacture and electrophoresis device using it

Abstract

The present invention provides a fluid device that exhibits lower flow path resistance than conventional fluid devices and more reliably prevents bubbles from adhering to the inner wall surfaces of a flow path. The fluid device provided by the present invention is a resin-constructed fluid device intended for mounting on an analyzer. The fluid device comprises an internal flow path with an aspect ratio (length-to-diameter ratio) of 10 or greater and a cross-section shaped like a circular tube. The mean arithmetic roughness of an inner wall surface of the flow path is 0.5 µm or less, and its standard deviation is 0.1 µm or less.

Description

Technical field

[0001] The present invention relates to a fluid device, a method for its manufacture and an electrophoresis device that uses it. background

[0002] Traditionally, a fluid apparatus installed in an analyzer consists of a mold mimicking the shape of a fine flow path being pressed onto a heat-softened resin substrate to transfer the shape, and then a plate-like cover being attached to the resin substrate (see, for example, patent literature 1). With this type of fluid apparatus, it is difficult to create a flow path with a substantially circular cross-section and low flow path resistance, and a bond strength greater than the internal pressure exerted on the flow path is required. Fluid apparatuses in which a substantially circular flow path is created by machining a resin substrate are also conceivable, as are fluid apparatuses in which a substantially circular flow path is created by a 3D printer.However, in these fluid devices, fine irregularities form on the inner wall surface of the flow path due to cut marks or scan marks. When such irregularities are present in the flow path, problems arise that impede fluid flow and cause air bubbles to adhere to the inner wall surface.

[0003] Meanwhile, technologies for smoothing irregularities on the surface of a resin substrate by applying an organic solvent or resin varnish to the surface of the resin substrate have been conventionally published (see, for example, patent literature 2 and 3).

[0004] Consequently, it might be possible to apply a substrate surface planarization technology (see, for example, patent literature 2 and 3) to a fluid device manufactured by machining or by a 3D printer. Bibliography Patent literature Patent Literature 1: Publication of the unexamined Japanese patent application (translation of PCT application) No. 2017-538416 Patent Literature 2: Publication of the unexamined Japanese patent application No. Hei6(1994)-128398 Patent Literature 3: Publication of the unexamined Japanese patent application No. Hei5(1993)-198952 Overview of the invention Technical problem

[0005] However, even when the smoothing technologies disclosed in patent literature 2 and 3 are applied to fluid devices manufactured by machining or 3D printing, a problem arises: for example, it is difficult for the organic solvent to penetrate a fine flow path. While it is conceivable to pressurize the vapor of the organic solvent and force it into the flow path, the pressurized organic solvent partially liquefies within the flow path. The liquefied organic solvent adheres to the inner wall surface of the flow path at several points along its length. This results in an uneven, rough surface along the length of the fluid device's flow path.This makes it even easier for bubbles to adhere to the inner wall surface of the fluid device's flow path.

[0006] To solve the problem mentioned above, the present invention provides a fluid device which has a lower flow path resistance than conventional fluid devices and more reliably prevents bubbles from adhering to the inner wall surface of the flow path, a method for its manufacture, and an electrophoresis device which uses it. Solution to the problem

[0007] According to one aspect of the present invention, a fluid device comprising a resin and intended for mounting on an analyzer is provided. The fluid device has an internally arranged flow path with an aspect ratio (length-to-diameter ratio) of 10 or more and a cross-section shaped like a circular tube. The mean arithmetic roughness of an inner wall surface of the flow path is 0.5 µm or less, and its standard deviation is 0.1 µm or less.

[0008] According to a further aspect of the present invention, a method for manufacturing the fluid device described above is provided. This method is used to manufacture a fluid device with an internally arranged flow path having an aspect ratio (length-to-diameter ratio) of 10 or more, a cross-section shaped like a circular tube, and a mean arithmetic roughness of the inner wall surface of the flow path of 0.5 µm or less with a standard deviation of 0.1 µm or less. The method comprises the steps of: forming a through-hole with a cross-section shaped like a circle in a resin block; and suctioning from one end of the through-hole while introducing the vapor of an organic solvent from the other end.

[0009] According to yet another aspect of the present invention, an electrophoresis device containing the fluid device described above is provided, and the flow path in the fluid device forms part of the flow path for electrophoresis. Advantageous effects of the invention

[0010] According to the aspects of the present invention described above, a fluid device which has a lower flow path resistance than conventional fluid devices and prevents bubbles from adhering to the inner wall surface of the flow path, a method for its manufacture and an electrophoresis device using it are provided. Brief description of the drawings Fig. Figure 1 is a representation illustrating a configuration of a fluid device according to a first embodiment of the present invention. Fig. 2 contains reference numerals 201 to 204, which represent the processes used in a method for producing the in Fig. The steps contained in the fluid device shown in Figure 1 illustrate and describe them. Fig. Figure 3 contains reference numerals 301 and 302. Reference numeral 301 designates a cross-sectional view along line IIIa-IIIa of the [reference number] in Fig. 2. Reference numeral 204. Reference numeral 302 designates a cross-sectional view illustrating a modified example of the flow path depicted in the representation designated by reference numeral 301. Fig. Figure 4 is a representation illustrating a configuration of the fluid device according to a second embodiment of the present invention. Fig. 5 contains reference numerals 501 to 504, which describe the process for manufacturing the in Fig. The steps contained in the fluid device shown in the diagram 4 illustrate and describe them. Fig. Figure 6 is a representation illustrating a configuration of the fluid device according to a third embodiment of the present invention. Fig. 7 contains reference numerals 701 to 704, which describe the process for manufacturing the in Fig. The steps contained in the fluid device shown in the diagram 6 illustrate and describe them. Fig. Figure 8 is a representation showing a configuration of an electrophoresis device that is described in Fig. Figure 6 illustrates the use of the fluid device shown. Fig. Figure 9 is a graph illustrating the results of the verification of the advantageous effects of the fluid device according to the embodiments of the present invention. Description of the embodiments

[0011] Methods (embodiments) for implementing the present invention will now be described in detail, with reference, where appropriate, to the accompanying drawings. A fluid device according to the embodiments of the present invention is to be mounted for use on an analyzer. The fluid device can be used with any analyzer that includes an electrophoresis apparatus, which will be described in detail later. In such an analyzer, the fluid device according to the embodiments of the present invention can function, for example, as a reagent liquid feeder, an optical cell, or a microreactor. First embodiment (fluid device)

[0012] Fig. Figure 1 is a representation illustrating a configuration of the fluid device 1A according to a first embodiment of the present invention.

[0013] As in Fig. Figure 1 shows that the fluid device 1A is also referred to as a polymer block and is a rectangular parallelepiped made of resin. As will be described later, the fluid device 1A, according to the present embodiment, uses a resin substrate 11 (see Figure 1). Fig. 3) as a base and has a flow path 23A formed therein. The resin substrate 11 corresponds to a “resin block”.

[0014] The flow path 23A has a circular cross-section and is tubular. Furthermore, in the present embodiment, it is assumed that the inner diameter of the flow path 23A is 0.5 mm or more and 5 mm or less. However, the inner diameter of the flow path 23A is not limited to this and can be suitably modified according to the properties of the fluid flowing through the flow path 23A.

[0015] Furthermore, the aspect ratio of inner diameter to length of the flow path 23A (length / inner diameter) is 10 or more.

[0016] Furthermore, the roughness of the inner wall surface of flow path 23A over its entire longitudinal area, measured as the mean arithmetic roughness (Ra) according to ISO 4287-1997, is 0.5 µm or less. Additionally, the standard deviation of the roughness of the inner wall surface of flow path 23A over its entire longitudinal area, measured as the mean square height (Sq) according to ISO 25178, is 0.1 µm or less.

[0017] The technical significance of the inner wall surface of the flow path 23A of the fluid device 1A according to the present embodiment with such surface roughness is explained in detail below together with the operational advantages of the fluid device 1A.

[0018] It is assumed that the resin forming the fluid device 1A according to the present embodiment is an acrylic resin. However, the resin forming the fluid device 1A is not limited to this, and a hard, transparent resin can also be used.

[0019] Examples of hard transparent resins include, but are not limited to, polycarbonate, polyarylate, polysulfone, polyimide, polyetherimide and norbornene resin. (Manufacturing process)

[0020] A method for manufacturing the fluid device 1A is now described (see Fig. 1) described.

[0021] Reference numbers 201 to 204 in Fig. 2 denote representations that are used in the processes for producing the in Fig. The steps contained in the fluid device 1A shown in the diagram illustrate this.

[0022] The manufacturing process according to the present embodiment comprises the following steps: providing the material in the Fig. 2. The resin substrate 11 (resin block) is shown in the illustration designated with reference numeral 201; forming a through-hole 20A with a circular cross-section in the resin substrate 11 (resin block), as shown in the illustration. Fig. 2, designated with reference numeral 202; and extraction from one end 21a of the through-hole 20A, while the vapor of an organic solvent is introduced from the other end 21b, as shown in the Fig. 2, the illustration designated with reference 203.

[0023] As a result, as in the Fig. 2, the representation designated by reference numeral 203, is indicated by arrow V INAs indicated, the vapor of the organic solvent passes through the through-hole 20A at a predetermined flow rate, which is at a reduced pressure lower than atmospheric pressure, and is, as indicated by arrow V OUT indicated, released from the resin substrate 11. In this case, the vapor of the organic solvent dissolves or softens, without liquefying, a fine, convex resin section (not shown) with a rough surface of the through-hole 20A, and flattens the convex section along the direction of the vapor flow. This smooths the rough surface of the through-hole 20A, which is formed with fine irregularities, and thus completes the fluid device 1A with the in Fig. 2 in the illustration designated with reference numeral 204, flow path 23A.

[0024] In the present embodiment, the resin substrate 11 (resin block) is assumed to have a rectangular parallelepiped shape in a top view, but this is not limited as long as the flow path 23A can be formed within it. Other three-dimensional shapes, such as a polygonal prism, a cylindrical shape, a disk shape, or a sphere, can also be used.

[0025] In the present embodiment, the method for forming the through-hole 20A in the resin substrate 11 (resin block) is assumed to be machining. Specifically, the assumed method consists of forming a hole (through-hole 20A) with a predetermined inner diameter by using a drill or other tool to linearly penetrate the resin substrate 11 (resin block).

[0026] The cross-sectional shape of this through hole 20A is in a shape that corresponds to the cross-sectional shape of the one in Fig. 1, flow path 23A is approaching, and the aspect ratio is 10 or more.

[0027] However, the method for forming the through-hole 20A in the resin substrate 11 (resin block) is not limited to machine processing, and as will be described later, the resin substrate 11 (resin block) with the through-hole 20A can also be formed by a 3D printer.

[0028] Method for introducing the vapor of the organic solvent into the through-hole 20A (see the in Fig. 2 (Representation designated with reference numeral 203) include, for example, a method of retaining the vapor of the organic solvent in the gas phase region of a tub which contains the organic solvent at the bottom, and of introducing the vapor of the organic solvent into the through-hole 20A.

[0029] If such a procedure is to be applied, it is desirable that saturated vapor of the organic solvent is generated in the gas phase region of the tub at a temperature lower than the temperature inside the through-hole 20A, that is, the temperature of the resin substrate 11 (resin block).

[0030] The following, for example, shows how in the Fig. Figure 2, designated with reference numeral 203, shows a vacuum pump being used to draw off fluid from one end 21a of the through-hole 20A, while the saturated vapor of the organic solvent is introduced from the other end 21b. This reduces the internal pressure acting on the through-hole 20A to a level lower than atmospheric pressure, thereby more reliably preventing the vapor of the organic solvent from liquefying inside the through-hole 20A.

[0031] In the present embodiment, it is assumed that the temperature of the resin substrate 11 (resin block) in the Fig. In step 2, designated with reference numeral 203, the temperature is room temperature (25 °C), but it can alternatively be set to a temperature higher than room temperature (25 °C). If such an alternative temperature setting is chosen, the temperature of the organic solvent in the gas phase region of the tank can be set to a temperature lower than the temperature of the resin substrate 11 (resin block) and higher than room temperature (25 °C).

[0032] In the present embodiment, it is assumed, but not limited to, that the organic solvent capable of dissolving or softening the acrylic resin from which the resin substrate 11 (resin block) is constructed is dichloromethane. Therefore, the organic solvent to be selected can be one that generates vapor capable of dissolving or softening the resin, depending on the type of resin from which the resin block is constructed, preferably at room temperature (25 °C) under atmospheric pressure.

[0033] Examples of such organic solvents include, but are not limited to, halogenated hydrocarbons with a low carbon number, ketones with a low carbon number (e.g., acetone), and cyclic ethers (e.g., tetrahydrofuran).

[0034] The in Fig. Figure 3, designated with reference numeral 301, is a cross-sectional view along line IIIa-IIIa of the [unclear text]. Fig. 2, the representation designated with reference numeral 204. The one in Fig. Figure 3, designated with reference numeral 302, is a cross-sectional view, which is a modified example of the one shown in the Fig. The flow path is illustrated in Figure 3, which is designated with reference numeral 301.

[0035] The manufacturing process described above smooths the inner wall surface of the through-hole 20A (see the in Fig. 2 (referencing 203) along the entire longitudinal direction and forms the flow path 23A with a circular cross-section and an aspect ratio of 10 or more within the in the Fig. 2 the illustration of resin substrate 11 (resin block) designated with reference numeral 204.

[0036] Furthermore, the in Fig. 3 in the illustration designated by reference numeral 301, the flow path 23A has an inner wall surface 23A1 which has a mean arithmetic roughness of 0.5 µm or less with a standard deviation of 0.1 µm or less.

[0037] Furthermore, as in the Fig. Figure 3, designated with reference numeral 302, shows the assumption that a coating layer L, which is composed of a SiO2 film, is to be formed on the inner wall surface 23A1 of the flow path 23A.

[0038] The SiO2 film used to coat the inner wall surface 23A1 is formed by applying a dispersion of SiO2 particles or a silicon alkoxide solution to the smoothed inner wall surface 23A1.

[0039] The SiO2 film, which utilizes a dispersion of SiO2 particles, is formed by evaporating a dispersion medium from the SiO2 particle dispersion applied to the inner wall surface 23A1. The SiO2 film, which utilizes a silicon alkoxide solution, is formed by a hydrolysis reaction of silicon alkoxide on the inner wall surface 23A1. Examples of silicon alkoxide include, but are not limited to, tetramethoxysilane, tetraethoxysilane, tetraisopropoxysilane, and tetrapropoxysilane. (Business advantages)

[0040] The following describes the operational advantages offered by the fluid device 1A according to the present embodiment and by the manufacturing process for it.

[0041] The fluid device 1A according to the present embodiment has a flow path 23A which is arranged internally and has an aspect ratio, which is the ratio of length to diameter, of 10 or more and a cross-section which is shaped like a circular tube, as well as a mean arithmetic roughness of the inner wall surface 23A1 of the flow path 23A of 0.5 µm or less with a standard deviation of 0.1 µm or less.

[0042] By having an aspect ratio of 10 or higher, the fluid device 1A allows a reagent and a sample (sample material) to remain in the flow path 23A for an extended period. As a result, the fluid device 1A is able to thoroughly mix the reagent and the sample (sample material) in the flow path 23A, enabling them to react. This effect is advantageous when the fluid device 1A functions as the liquid feed section of an analyzer or as a microreactor.

[0043] Furthermore, since the cross-sectional shape of the flow path 23A is circular, the flow path 23A in the fluid device 1A, unlike conventional fluid devices (see, for example, patent literature 1), has no corners. This allows the fluid device 1A to reduce the flow path resistance as the fluid flows through the flow path 23A. Moreover, because the flow path 23A in the fluid device 1A has no corners, the fluid device 1A prevents bubbles from adhering to the inner wall surface without impeding the fluid flow.

[0044] Since the fluid device 1A also has a mean arithmetic roughness of the inner wall surface of the flow path 23A of 0.5 µm or less with a standard deviation of 0.1 µm or less, the fluid device 1A is not only able to prevent bubbles from adhering to the inner wall surface of the flow path 23A, but also the adhesion of chemical compounds contained in the reagent and biological materials contained in the sample. Therefore, the fluid device 1A is able to prevent a reduction in the reliability of analytical results due to a gradual accumulation of chemical compounds and biological materials on the inner wall surface of the flow path 23A. Furthermore, the fluid device 1A is also able to more reliably prevent the chemical compounds and biological materials from gradually accumulating and blocking the flow path 23A.

[0045] Furthermore, if the surface roughness of the inner wall surface of the flow path is locally high, unlike that of the fluid device 1A according to the present embodiment, chemical compounds and biological materials can easily adhere to such sections. These chemical compounds and biological materials gradually accumulate, thereby reducing the reliability of the analysis results and blocking the flow path.

[0046] These effects are advantageous when the fluid device 1A functions as a liquid supply part of an analyzer or as a microreactor.

[0047] Furthermore, light scattering at the surface of flow path 23A in fluid device 1A is reduced because the mean arithmetic roughness of the inner wall surface of flow path 23A is 0.5 µm or less. This improves the reaction efficiency of the photochemical reaction between reagent and sample in flow path 23A within fluid device 1A. Additionally, fluid device 1A improves the light detection sensitivity of luminescence reactions within flow path 23A because the mean arithmetic roughness of the inner wall surface of flow path 23A is 0.5 µm or less.

[0048] These effects are advantageous when the fluid device 1A functions as an optical cell in an analyzer.

[0049] Furthermore, it is desirable that acrylic resin be used as the resin for the fluid device 1A described above.

[0050] The fluid device 1A described above provides excellent formability of the resin substrate 11 (resin block), which serves as the base, and facilitates machining to form the through-hole 20A in the resin substrate 11. Furthermore, the fluid device 1A expands the range of organic solvents that can be introduced into the through-hole 20A.

[0051] Furthermore, in the fluid device 1A described above, it is desirable that the inner wall surface 23A1 of the flow path 23A is coated with a SiO2 film.

[0052] The fluid device 1A increases the surface energy of the flow path 23A and is therefore able to prevent the adhesion of bubbles more reliably.

[0053] Furthermore, the manufacturing process for the fluid device 1A described above is a method for producing the fluid device 1A with the flow path 23A, which is arranged internally and has an aspect ratio (length-to-diameter ratio) of 10 or more, a cross-section shaped like a circular tube, and a mean arithmetic roughness of the inner wall surface of the flow path 23A of 0.5 µm or less with a standard deviation of 0.1 µm or less. This process includes the following steps: forming a through-hole 20A with a circular cross-sectional shape in the base formed by the resin substrate 11 (resin block); and suction from one end 21a of the through-hole 20A while introducing the vapor of the organic solvent from the other end 21b.

[0054] The manufacturing process described above introduces the vapor of the organic solvent into the through-hole 20A by suction from one end 21a of the through-hole 20A. Therefore, the vapor of the organic solvent can be easily introduced into a through-hole 20A even with a high aspect ratio of 10 or more.

[0055] Furthermore, the manufacturing process described above introduces the vapor of the organic solvent into the through-hole 20A by drawing it from one end 21a of the through-hole 20A. Therefore, the vapor of the organic solvent is introduced into the through-hole 20A at a reduced pressure, which is below atmospheric pressure. This prevents the vapor of the organic solvent from liquefying inside the through-hole 20A. The flow path 23A is smoothed over its entire longitudinal region without deformation.

[0056] Furthermore, the manufacturing process described above is able to reliably and efficiently produce the fluid device 1A with the flow path 23A, which is arranged internally and has an aspect ratio, which is the ratio of length to diameter, of 10 or more and a cross-section that is shaped like a circular tube, as well as a mean arithmetic roughness of the inner wall surface of the flow path 23A of 0.5 µm or less with a standard deviation of 0.1 µm or less.

[0057] Furthermore, when the manufacturing process for the fluid device 1A described above is applied, it is desirable that the through-hole 20A be formed by machining the resin substrate 11 (resin block).

[0058] The manufacturing process described above is able to form the through-hole 20A with a predetermined aspect ratio in the resin substrate 11 (resin block) simply and precisely.

[0059] Additionally, when the manufacturing process for the fluid device 1A described above is applied, it is desirable that acrylic resin be used as the resin substrate 11 (resin block) forming the resin and that dichloromethane be used as the organic solvent.

[0060] The manufacturing process described above is able to efficiently form the flow path 23A in the resin substrate 11 (resin block) which has a mean arithmetic roughness of the inner wall surface of 0.5 µm or less with a standard deviation of 0.1 µm or less.

[0061] Furthermore, it is desirable that the manufacturing process for the fluid device 1A described above additionally includes a step of forming a SiO2 film on the inner wall surface of the through-hole 20A after the step of introducing the vapor of the organic solvent into the through-hole 20A.

[0062] The manufacturing process described above is capable of forming the flow path 23A by using the SiO2 film with a high surface energy. This more reliably prevents bubbles from adhering to the flow path 23A of the fluid device 1A. Second embodiment (fluid device)

[0063] The fluid device according to a second embodiment will now be described.

[0064] Components that are compatible with those of fluid device 1A (see Fig. 1) Components that are identical according to the first embodiment are designated by the same reference numerals as the corresponding components in the first embodiment and are not described redundantly in detail.

[0065] Fig. Figure 4 is a representation illustrating a configuration of the fluid device 1B according to the second embodiment of the present invention.

[0066] As in Fig. As shown in Figure 4, the fluid device 1B is configured such that a flow path 23B, formed within the acrylic resin-based resin substrate 11 (resin block), is curved. However, the material of the resin substrate 11 is not limited to acrylic resin. The aforementioned hard transparent resin can also be used for the resin substrate 11.

[0067] The flow path 23B comprises a first flow path section 23B1 and a second flow path section 23B2. These flow path sections 23B1 and 23B2 are both linear. The first flow path 23B1 and the second flow path 23B2 communicate within the resin substrate 11 (resin block) such that they form an enclosed angle with each other. Consequently, the flow path 23B forms a single hole integrating the first flow path section 23B1 and the second flow path section 23B2 to create a single hole that penetrates the resin substrate 11 (resin block).

[0068] The angle formed between the first flow path section 23B1 and the second flow path section 23B2 can be set in the range of more than 0 degrees and less than 180 degrees. In the present embodiment, however, the angle formed between the first flow path section 23B1 and the second flow path section 23B2 is set to 120 degrees.

[0069] The cross-sectional shape of the flow path 23B described above is circular. In the present embodiment, it is assumed that the inner diameter of the flow path 23B is 0.5 mm or more and 5 mm or less. However, the inner diameter of the flow path 23B is not limited to this and can be suitably modified according to the properties of the fluid flowing through the flow path 23B.

[0070] Furthermore, the aspect ratio of inner diameter to length of the flow path 23B (length / inner diameter) is 10 or more.

[0071] Furthermore, the roughness of the inner wall surface of flow path 23B over its entire longitudinal area, measured as mean arithmetic roughness (Ra) according to ISO 4287-19970, is 0.5 µm or less. Additionally, the standard deviation of the roughness of the inner wall surface of flow path 23B over its entire longitudinal area, measured as mean square height (Sq) according to ISO 25178, is 0.1 µm or less.

[0072] Furthermore, it is assumed that the coating layer L, which is composed of a SiO2 film (see the illustration designated with reference numeral 302 in Fig. 3) is provided on the inner wall surface of flow path 23B for coating the inner wall surface. (Manufacturing process)

[0073] A method for manufacturing the fluid device 1B is now described (see Fig. 4) described.

[0074] Reference numbers 501 to 504 in Fig. 5 denote representations that are used in the process for producing the in Fig. The steps contained in the fluid device 1B shown in Figure 4 illustrate the process.

[0075] The manufacturing process according to the present embodiment comprises the following steps: providing the material in the Fig. 5 with reference numeral 501, the resin substrate 11 (resin block) is shown; forming a through hole 20B with a circular cross-section in the resin substrate 11 (resin block) as shown in the illustration. Fig. Figure 5, designated with reference numeral 502, illustrates this; and extraction from one end 21c of the through-hole 20B, while the vapor of the organic solvent is introduced from the other end 21d, as shown in Figure 5. Fig. Figure 5 is shown with reference numeral 503.

[0076] In the step of forming the through hole 20B, which is located in the Fig. As shown in Figure 5 with reference numeral 502, the curved through hole 20B is formed from both one end 21c and the other end 21d using a drill or other tool.

[0077] Furthermore, during the step of introducing the vapor of the organic solvent into the through-hole 20B, as shown in Fig. 5, the representation designated by reference numeral 503, is indicated by arrow V IN indicated, with a predetermined flow rate through the through-hole 20B, which is at a pressure reduced compared to atmospheric pressure, and is, as indicated by arrow V OUTindicated, released from the resin substrate 11. In this case, the vapor of the organic solvent dissolves or softens the fine, convex resin section (not shown) of a rough surface of the through-hole 20B without liquefying itself, and flattens the convex section along the direction of the vapor flow. This smooths the rough surface of the through-hole 20B, which is formed with fine irregularities, and thus completes the fluid device 1B with the one shown in the Fig. Flow path 23B is shown in illustration 5, which is designated with reference numeral 504. (Business advantages)

[0078] The following describes the operational advantages provided by the fluid device 1B according to the present embodiment and by the manufacturing process for it.

[0079] The fluid device 1B according to the present embodiment has an internally arranged flow path 23B with an aspect ratio (length-to-diameter ratio) of 10 or more, a cross-section shaped like a circular tube, and a mean arithmetic roughness of the inner wall surface of the flow path 23B of 0.5 µm or less with a standard deviation of 0.1 µm or less. Therefore, the fluid device 1B is able to provide operational advantages similar to those offered by the fluid device 1A according to the first embodiment described above.

[0080] Furthermore, the flow path 23B of the fluid device 1B is curved according to the present embodiment.

[0081] Fluid device 1B and its manufacturing process are capable of changing the locations of the fluid outlet and inlet of flow path 23B of fluid device 1B by altering the angle formed between the first flow path section 23B1 and the second flow path section 23B2. Fluid device 1B and its manufacturing process are capable of producing a fluid device that offers greater freedom in its placement within an analyzer. Third embodiment (fluid device)

[0082] The fluid device according to a third embodiment will now be described.

[0083] Components that are compatible with those of fluid device 1A (see Fig. 1) Components that are identical according to the first embodiment are designated by the same reference numerals as the corresponding components in the first embodiment and are not described redundantly in detail.

[0084] Fig. Figure 6 is a representation illustrating a configuration of a fluid device 1C according to the third embodiment of the present invention.

[0085] As in Fig. As shown in Figure 6, the fluid device 1C has a flow path 23C formed within the resin substrate 11 (resin block) made of acrylic resin. However, the material of the resin substrate 11 is not limited to acrylic resin. The hard transparent resin mentioned above can also be used for the resin substrate 11.

[0086] In the present embodiment, the resin substrate 11 (resin block) is shaped like a rectangular parallelepiped.

[0087] The flow path 23C contains several flow paths, namely a flow path 23C1, a flow path 23C2, a flow path 23C3 and a flow path 23C4.

[0088] Furthermore, the fluid device 1C has concave sections 24 and 25 that are open towards the surface of the resin substrate 11 (resin block). These two concave sections 24 and 25 are formed as cylindrical spaces with bottoms. The surface towards which concave section 24 is open and the surface towards which concave section 25 is open are arranged perpendicular to each other.

[0089] The flow path 23C1 extends linearly from a side surface of the resin substrate 11 towards the interior of the resin substrate 11.

[0090] Flow path 23C2 has its base end at one end of flow path 23C1 formed inside the resin substrate 11 and extends in such a way that it forms an L-shape with flow path 23C1. The tip of flow path 23C2 is connected to the bottom surface of the concave section 25.

[0091] The flow path 23C3 extends linearly from one end connected to the bottom surface of the concave section 25 towards the interior of the resin substrate 11. The other end of the flow path 23C3 is connected to the inner circumferential surface of the concave section 24.

[0092] The flow path 23C4 extends linearly from a side surface of the resin substrate 11 towards the interior of the resin substrate 11, so that it runs parallel to the flow path 23C1 and is connected to the inner circumferential surface of the concave section 24.

[0093] That is, the fluid device 1C is designed such that the flow path 23C is formed by the several flow paths, namely the flow path 23C1, the flow path 23C2, the flow path 23C3 and the flow path 23C4, and that the flow path 23C2 and the flow path 23C3 communicate with each other via the concave section 25, and furthermore, that the flow path 23C3 and the flow path 23C4 communicate with each other via the concave section 24.

[0094] The cross-sectional shape of the flow path 23C described above is circular. In the present embodiment, the inner diameter of the flow path 23B is assumed to be 0.5 mm or more and 5 mm or less. However, the inner diameter of the flow path 23C is not limited to this and can be suitably modified according to the properties of the fluid flowing through the flow path 23C.

[0095] Furthermore, the aspect ratio of inner diameter to length of the flow path 23C (length / inner diameter) is 10 or more.

[0096] Furthermore, the roughness of the inner wall surface of flow path 23C over its entire longitudinal area, measured as mean arithmetic roughness (Ra) according to ISO 4287-1997, is 0.5 µm or less. Additionally, the standard deviation of the roughness of the inner wall surface of flow path 23C over its entire longitudinal area, measured as mean square height (Sq) according to ISO 25178, is 0.1 µm or less.

[0097] Furthermore, the mean arithmetic roughness (Ra) of the inner wall surfaces of the concave sections 24, 25 is also 0.5 µm or less, and their mean square height (Sq) is 0.1 µm or less.

[0098] Furthermore, it is assumed that the coating layer L, which is composed of a SiO2 film (see the in Fig. 3 (referencing 302) is provided on the inner wall surface of the flow path 23C and on the inner wall surfaces of the concave sections 24, 25 for the purpose of coating such inner wall surfaces. (Manufacturing process)

[0099] A method for manufacturing the fluid device 1C is now described (see Fig. 6) described.

[0100] Reference numbers 701 to 704 in Fig. 7 denote representations that are used in the process for producing the in Fig. The steps contained in the fluid device 1C shown in Figure 6 illustrate the process.

[0101] The manufacturing process according to the present embodiment comprises the following steps: providing the material in the Fig. 7, designated with reference numeral 701, of the resin substrate 11 (resin block) shown; forming a through hole 20C with a circular cross-section in the resin substrate 11 (resin block), as shown in the illustration in Fig. Figure 7, designated with reference numeral 702, shows the extraction from one end 21e, 21f, 21g of the through-hole 20C while the vapor of the organic solvent is introduced into the through-hole 20C, as shown in the illustration in Fig. Figure 7 is shown with reference numeral 703.

[0102] The manufacturing process described above forms the concave sections 24, 25 in the resin substrate 11 using a drill or other tool. Subsequently, the through-hole 20C with a circular cross-section is formed by machining. During the step of forming the in the Fig. In Figure 7, reference numeral 702, a through hole 20C1 is drilled and formed from one end 21h of the through hole 20C1 using a drill or other tool. A through hole 20C2 is drilled and formed from one end 21e of the through hole 20C2 using a drill or other tool. A through hole 20C3 is drilled and formed from one end 21f of the through hole 20C3 using a drill or other tool. A through hole 20C4 is drilled and formed from one end 21g of the through hole 20C4 using a drill or other tool.

[0103] Furthermore, during the process of introducing the vapor of the organic solvent into the through-hole 20C, as described in Fig. Figure 7, reference numeral 703, shows that through-holes 20C1, 20C2, and through-hole 20C3 are drawn through the concave section 25, which communicates between one end 21e of through-hole 20C1 and one end 21f of through-hole 20C3. Through-hole 20C4 is drawn through one end 21g. Consequently, the vapor of the organic solvent is drawn from the concave section 24 and one end 21h of through-hole 20C1 at a reduced pressure lower than atmospheric pressure, as shown in the figure. Fig. 7, the representation designated with reference numeral 703, is replaced by reference numeral V IN specified, introduced and is formed from the concave section 25 and the one end 21g of the through hole 20C4, as indicated by the reference numeral V OUT stated, submitted.

[0104] In the above case, the vapor of the organic solvent dissolves or softens the fine, convex resin section (not shown) of a rough surface of the through-hole 20C (see the in Fig. 7 (referencing figure 702) without liquefying, and flattens the convex section along the direction of the steam flow. This smooths the rough surface of the through-hole 20C (see the illustration in Fig. 7 (referencing figure 702), which is formed with fine irregularities, and thus completes the fluid device 1C (see Fig. 6) with the one in the Fig. The flow path 23C is shown in illustration 7, which is designated with reference numeral 704. (Electrophoresis device)

[0105] An electrophoresis device is now used, which includes the fluid device 1C (see Fig. 6) as described in the present embodiment.

[0106] Fig. Figure 8 is a diagram illustrating a configuration of a capillary electrophoresis device Ap (hereafter referred to simply as "electrophoresis device Ap"), which is an example of the analyzer. The electrophoresis device Ap is used, for example, as a DNA sequencer or DNA fragment analyzer.

[0107] As in Fig. Figure 8 shows that the electrophoresis apparatus Ap essentially comprises the fluid apparatus 1C, a capillary 26, a reagent bottle 31, a container 32, a container 33, a high-voltage power supply 50 and a laser light source 60.

[0108] The capillary 26 has a sample inlet end 26a and a capillary main body 26b, which has the sample inlet end 26a at one end. The other end of the capillary main body 26b is designed to be immersed in a reagent, described later, which is to be filled into the container 32. The sample inlet end 26a is attached to the concave section 25 of the fluid device 1C. A cathode 52 is arranged inside the container 32.

[0109] The reagent bottle 31 is attached via a valve 41 to one end 21g of the flow path 23C4 of the fluid device 1C. It is assumed that in the present embodiment, the reagent bottle 31 contains a buffer gel as the reagent.

[0110] Container 33 is attached to one end 21h of flow path 23C1 via a valve 43.

[0111] Container 33 is to be filled with the reagent from reagent bottle 31, as will be described later.

[0112] An anode 53 is arranged in the container 33. The power supply 50 applies a voltage between the cathode 52 and the anode 53.

[0113] The laser light source 60 irradiates a predetermined position 62 of the main capillary body 26b with a laser beam 61.

[0114] The processes carried out by the electrophoresis device Ap are now mainly referred to below. Fig. 8 described.

[0115] The fluid device 1C of the electrophoresis device Ap functions as a liquid supply device for the reagent stored in the reagent bottle 31.

[0116] A motor-driven piston pump (not shown) is attached to the opening of the concave section 24 of the fluid device 1C.

[0117] In the electrophoresis apparatus Ap, the reagent in the reagent bottle 31 is filled into the concave section 24 when the piston pump is controlled with the sample feed end 26a closed, valve 43 closed and valve 41 open, so that it aspirates.

[0118] Subsequently, when the piston pump, with valve 41 closed and sample feed end 26a open, is actuated to force the reagent out of the concave section 24, the reagent is transferred through the capillary 26 into the container 32. Repeating the above process as needed, the container 32 is filled with reagent until the tip of the capillary 26 is immersed. The cathode 52 is immersed in the reagent. The hollow part of the capillary 26 is then completely filled with the reagent.

[0119] Next, in the electrophoresis apparatus Ap, the reagent is filled from the reagent bottle 31 into the concave section 24 when the piston pump is controlled to draw off the sample with the sample feed end 26a closed, valve 43 closed and valve 41 open.

[0120] Subsequently, when the piston pump is controlled with valve 41 closed and valve 43 open, the reagent in the concave section 24 is transferred via flow path 23C3, flow path 23C2 and flow path 23C1 to container 33, and then through capillary 26. If the above process is repeated as required, at least container 33, flow paths 23C1, 23C2 and the concave section 25 are filled with the reagent.

[0121] Subsequently, when the valve 43 and the sample feed end 26a are open, an electrophoresis path is formed between the cathode 52 and the anode 53 via the reagent.

[0122] When an analytical procedure using the electrophoresis device Ap is applied, a sample containing, for example, several types of ingredients (such as DNA fragments) labeled with several types of fluorescent dyes is injected into the sample feed end 26a.

[0123] Subsequently, when a voltage is applied between the cathode 52 and the anode 53, negatively charged components (such as DNA fragments) contained in the sample are electrophoretized in the main capillary body 26b from the sample feed end 26a towards the container 32.

[0124] Due to differences in mobility during electrophoresis, several types of components contained in the sample are successively separated. Position 62 of the main capillary body 26b, reached after electrophoresis over a predetermined distance, is irradiated with the laser beam 61 emitted by the laser light source 60. The laser beam 61 excites fluorescence emission by the several types of phosphors labeled by the components successively passing position 62. The fluorescence, which changes over time as a result of the electrophoresis, is measured by a multi-color detection device (not shown) that detects light with several wavelength bands. (Business advantages)

[0125] The following describes the operational advantages provided by the fluid device 1C according to the present embodiment, by the manufacturing process for it, and by the electrophoresis device using it.

[0126] The fluid device 1C according to the present embodiment is a resin-constructed fluid device 1C contained within an analyzer and comprising several internally arranged flow paths 23C1, 23C2, 23C3, 23C4, which have an aspect ratio (length-to-diameter ratio) of 10 or more and a circular, tubular cross-sectional shape. The flow paths 23C1, 23C2, 23C3, 23C4 exhibit a mean arithmetic roughness of the inner wall surfaces of 0.5 µm or less with a standard deviation of 0.1 µm or less.

[0127] The fluid device 1C described above has an internally arranged flow path 23C with an aspect ratio (length-to-diameter ratio) of 10 or more, a cross-section shaped like a circular tube, and a mean arithmetic roughness of the inner wall surface of the flow path 23C of 0.5 µm or less with a standard deviation of 0.1 µm or less. Therefore, the fluid device 1C is able to offer operational advantages similar to those provided by the fluid device 1A according to the first embodiment described above.

[0128] Furthermore, the fluid device 1C has several flow paths 23C1, 23C2, 23C3, 23C4 and thus enables a diversification of the fluid flow paths within the fluid device 1C.

[0129] Furthermore, it is desirable to use acrylic resin as the resin for the fluid device 1C described above.

[0130] The fluid device 1C described above provides excellent formability of the resin substrate 11 (resin block), which serves as the base, and facilitates machining to form the through-hole 20C in the resin substrate 11. Furthermore, the fluid device 1C expands the range of organic solvents that can be introduced into the through-hole 20C.

[0131] Furthermore, in the fluid device 1C described above, it is assumed that the inner wall surface of the flow path 23C is coated with a SiO2 film.

[0132] The fluid device 1C increases the surface energy of the inner wall surface of the flow path 23C and is therefore able to prevent the adhesion of bubbles more reliably.

[0133] Furthermore, in the fluid device 1C described above, it is assumed that the inner wall surfaces of the concave sections 24, 25 are coated with a SiO2 film.

[0134] The fluid device 1C increases the surface energy of the inner wall surfaces of the concave sections 24, 25 and is thus able to prevent the adhesion of bubbles more reliably.

[0135] Furthermore, in the fluid device 1C described above, the flow path 23C is composed of holes penetrating the resin substrate 11 (resin block) and is provided with concave sections 24, 25 that are open to the surface of the resin substrate 11 (resin block). At least two of the several flow paths 23C, namely flow path 23C2 and flow path 23C3, communicate with each other via the concave section 25. Additionally, flow path 23C3 and flow path 23C4 communicate with each other via the concave section 24.

[0136] The fluid device 1C makes it possible to increase the length of the flow path within the resin substrate 11 (resin block). This allows the fluid to remain in the resin substrate 11 (resin block) for a longer period of time.

[0137] Furthermore, in the fluid device 1C described above, several concave sections (concave sections 24, 25) are formed in the resin substrate 11 (resin block).

[0138] The fluid device 1C allows various parts and devices such as pumps and capillaries to be easily mounted via the concave sections 24, 25.

[0139] The manufacturing process for the fluid device 1C described above includes the following steps: providing the resin substrate 11 (resin block); forming the through-hole 20C with a circular cross-section in the resin substrate 11 (resin block); and suctioning from one end 21e, 21f, 21g of the through-hole 20C while the vapor of an organic solvent is introduced into the through-hole 20C.

[0140] Furthermore, when the manufacturing process described above is applied, the through-hole 20C is formed by machine processing of the resin substrate 11 (resin block).

[0141] The manufacturing process described above forms the concave sections 24, 25 in the resin substrate 11 using a drill or other tool. Subsequently, the through-hole 20C, which has a circular cross-section, is formed by machining.

[0142] Drill marks are then formed on the inner wall surfaces of the through-hole 20C and the concave sections 24, 25. When the fluid flows through the through-hole 20C in the condition described above, bubbles tend to adhere to the inner wall surfaces.

[0143] Consequently, in the manufacturing process according to the present embodiment, suction is carried out from one end 21e, 21f, 21g of the through-hole 20C, while the vapor of the organic solvent is introduced into the through-hole 20C. Furthermore, the suction at one end 21e, 21f is carried out through the concave section 25. The vapor of the organic solvent is introduced through the concave section 24 into the through-holes 20C3, 20C4.

[0144] As a result, the inner wall surfaces of the flow path 23C and the concave sections 24, 25 are smoothed and rendered free of roughness. The manufacturing process described above is capable of producing the fluid device 1C, which has the flow path 23C with an inner wall surface exhibiting low surface roughness.

[0145] Furthermore, when the manufacturing process described above is used, it is desirable that acrylic resin be used as the resin forming the resin block and that dichloromethane be used as the organic solvent.

[0146] The manufacturing process described above is able to efficiently form the flow path 23C in the resin substrate 11 (resin block) with a mean arithmetic roughness of the inner wall surface of 0.5 µm or less and a standard deviation of 0.1 µm or less.

[0147] Furthermore, it is desirable that the manufacturing process described above, after forming the flow path 23C, additionally includes a step of forming a SiO2 film on the inner wall surface of the through-hole 20C by introducing the vapor of the organic solvent into the through-hole 20C.

[0148] The manufacturing process described above is capable of forming the flow path 23C, which has a high surface energy, by using the SiO2 film. This more reliably prevents bubbles from adhering to the flow path 23C of the fluid device 1C.

[0149] Furthermore, the electrophoresis device Ap according to the present embodiment includes the fluid device 1C, and the flow paths 23C1, 23C2 in the fluid device 1C form part of the flow paths for electrophoresis.

[0150] The electrophoresis device Ap, which is located in the Fig. In the machine-processed resin substrate 11 (resin block) shown in Figure 7, reference numeral 702, bubbles mixed in at the time of reagent aspiration adhere to the inner wall surface of the through-hole 20C, which serves as the flow path. As a result, the electrophoresis to be carried out through the through-hole 20C is hindered. Furthermore, if the added bubbles are large enough to block the through-holes 20C, the cathode 52 and the anode 53 can be damaged.

[0151] In contrast, the fluid device 1C used for the electrophoresis device Ap according to the present embodiment does not allow the bubbles to adhere to the inner wall surface of the flow path 23C. Even if large bubbles enter the flow path 23C, they are forced out into the containers 32, 33.

[0152] Furthermore, the electrophoresis device Ap, which utilizes the fluid device 1C featuring a SiO2 film on the inner wall surfaces of the flow path 23C and the concave sections 24 and 25, is able to increase the surface energy of such inner wall surfaces and thus more effectively prevent bubble adhesion. This enables the electrophoresis device Ap to perform even better electrophoresis. Examples

[0153] The following describes the results of verifying the advantageous effects of the fluid device according to the embodiments of the present invention. (Example 1)

[0154] For example 1, a Fig. 4 Fluid device 1B shown is manufactured by using acrylic resin.

[0155] First, in a prepared resin substrate 11 (see the in Fig. 5 (referencing 501) by machining with a drill a curved through hole 20B (see the in Fig. Figure 5 (reference 502) was formed. The through-hole 20B had a length of 50 mm and an inner diameter of 0.8 mm. The aspect ratio (length / inner diameter) of the through-hole 20B was 62.5.

[0156] Subsequently, as in the Fig. Figure 5, designated with reference numeral 503, shows that a suction was carried out from one end 21c of the through-hole 20B, while the dichloromethane vapor was introduced from the other end 21d. As a result, the fluid device 1B (see the illustration in Figure 5) was exposed to the fluid. Fig. 5, designated with reference numeral 504), which shows a flow path 23B (see the one in Fig. 5 (referencing representation 504) with a smoothed inner wall surface, obtained.

[0157] The length of one end of flow path 23B, which served as a steam inlet, and the roughness of the inner wall surface at a position corresponding to this length were measured in terms of mean arithmetic roughness (Ra) according to ISO 4287-1997. The measurement results are shown in the graph in Fig. 9 shown.

[0158] The horizontal axis in Fig. 9 represents the position at one end of flow path 23B; that is, the position of the dichloromethane vapor inlet end is considered to be 0 mm, and the position at the other end of flow path 23B is considered to be 50 mm. The vertical axis in Fig. 9 represents the measured surface roughness [µm] (mean arithmetic roughness (Ra)) of the inner wall surface.

[0159] In Fig. 9. The surface roughness [µm] of Example 1, indicated by circular markings, is concentrated at approximately 0.1 µm or less over the entire longitudinal length of flow path 23B. Although the maximum surface roughness [µm] value (0.4 µm) is specified as part of the 5 mm or less range in terms of the length of the dichloromethane vapor inlet end, the surface roughness [µm] is 0.5 µm or less. The standard deviation of the inner wall surface roughness over the entire longitudinal length of flow path 23B, measured in terms of mean square height (Sq) according to ISO 25178, was 0.1 µm or less. (Comparative example 1)

[0160] For comparative example 1, a fluid device was obtained by adding the resin substrate 11, in which the in the Fig. The through-hole 20B shown in the drawing with reference numeral 502 was exposed in a tub to the vapor of dichloromethane without drawing off from one end 21 of the through-hole 20B.

[0161] Regarding the fluid device mentioned above, the surface roughness [µm] of the inner wall surface of a flow path was measured in the same way as in Example 1. The measurement results are shown in the graph of Fig. 9 represented by square markings. As in Fig. Figure 9 shows that the surface roughness [µm] of the flow path in the fluid device of comparison example 1 was 0.5 µm or more along its entire length. This means that the inner wall surface of the flow path was not smoothed. Furthermore, the mean square height (Sq) according to ISO 25178 exceeded 0.1 µm. (Comparative example 2)

[0162] For comparative example 2, the resin substrate 11 with the through-hole 20B formed therein, as in the Fig. Figure 5, designated with reference numeral 502, shows the inner wall surface of the through-hole 20B being reamed. For Comparative Example 2, the resin substrate 11 was not brought into contact with the vapor of dichloromethane, and the through-hole 20B was merely reamed to create a fluid device.

[0163] Regarding the fluid device mentioned above, the surface roughness [µm] of the inner wall surface of a flow path was measured in the same way as in Example 1. The measurement results are shown in the graph of Fig. 9 represented by triangular markings. As in Fig. Figure 9 shows that the surface roughness [µm] of the flow path in the fluid device of comparison example 2 was 0.5 µm or less at some points. However, the surface roughness [µm] varied considerably, and the standard deviation was 0.2 µm or more. In particular, the surface roughness and the standard deviation varied considerably from a length of 8 mm, which exceeded an aspect ratio of 10. (Evaluation results)

[0164] The fluid apparatus of comparison example 1 was produced simply by exposing the resin substrate 11 to dichloromethane vapor. Therefore, it was confirmed that the inner wall surface of the through-hole 20B could not be smoothed.

[0165] Furthermore, the flow path of the fluid device in comparison example 1 was formed solely by mechanically polishing the through-hole 20B. Therefore, it was confirmed that the surface roughness [µm] varied considerably.

[0166] In contrast, the fluid device 1B of Example 1 of the present invention (see the illustration in Fig. Figure 5 (reference 504) shows that a suction was performed from one end of the through-hole while the vapor of the organic solvent was introduced from the other end. Consequently, it was confirmed that the mean arithmetic roughness of the inner wall surface of flow path 23B was 0.5 µm or less and that its standard deviation was 0.1 µm or less.

[0167] Although the embodiments of the present invention have been described above, the present invention is not limited to the embodiments described above. Various modifications can be made without departing from the concept and scope of the present invention.

[0168] In the fluid devices 1A, 1B, 1C described in connection with the preceding embodiments, namely the first to third embodiments, the through-holes 20A, 20B, 20C are formed by machining the resin substrate 11 (resin block). However, the fluid devices 1A, 1B, 1C with the through-holes 20A, 20B, 20C can also be manufactured by a 3D printer. Furthermore, the preceding description assumes that the electrophoresis device Ap according to the embodiments uses the flow path 23C of the fluid device 1C according to the third embodiment as part of the flow path for electrophoresis. However, the flow paths 23A, 23B of the fluid devices 1A, 1B can also be used as part of the flow path for electrophoresis. Reference symbol list 1A, 1B, 1C Fluid device 20A, 20B, 20C Through hole 23A, 23B, 23C Flow path 11 Resin substrate (resin block) 24, 25 Concave Section Ap electrophoresis device QUOTES INCLUDED IN THE DESCRIPTION

[0000] This list of documents cited by the applicant was automatically generated and is included solely for the reader's convenience. The list is not part of the German patent or utility model application. The DPMA accepts no liability for any errors or omissions. Cited patent literature

[0000] JP 2017-538416

[0004] JP 128398

[0004] JP 198952

[0004]

Claims

[1] Fluid device made of resin and intended to be mounted on an analyzer, wherein the fluid device comprises: a flow path that is arranged internally and has an aspect ratio, namely the ratio of length to diameter, of 10 or more, and a cross-section that is shaped like a circular tube; where the mean arithmetic roughness of an inner wall surface of the flow path is 0.5 µm or less and its standard deviation is 0.1 µm or less. [2] Fluid device according to claim 1, wherein acrylic resin is used as the resin. [3] Fluid device according to claim 1, wherein the inner wall surface of the flow path is coated with a SiO2 film. [4] Fluid device made of resin and intended to be mounted on an analyzer, wherein the fluid device comprises: a flow path that is arranged internally and has an aspect ratio, namely the ratio of length to diameter, of 10 or more, and a cross-section that is shaped like a circular tube; where the mean arithmetic roughness of an inner wall surface of the flow path is 0.5 µm or less and its standard deviation is 0.1 µm or less. [5] Fluid device according to claim 4, wherein acrylic resin is used as the resin. [6] Fluid device according to claim 4, wherein the inner wall surface of the flow path is coated with a SiO2 film. [7] Fluid device according to claim 4, the flow path is composed of holes that penetrate a resin block; the resin block is provided with concave sections that are open towards the surface of the resin block; and At least two of the several flow paths communicate with each other via the concave sections. [8] Fluid device according to claim 7, wherein several of the concave sections are formed in the resin block. [9] Electrophoresis apparatus which includes: the fluid device according to claim 1 or 4; where the flow path in the fluid device forms part of the flow path for electrophoresis. [10] Electrophoresis apparatus according to claim 9, wherein acrylic resin is used as the resin. [11] Electrophoresis apparatus according to claim 9, wherein the inner wall surface of the flow path is coated with a SiO2 film. [12] Electrophoresis apparatus according to claim 7, wherein the inner wall surfaces of the concave wall sections are coated with a SiO2 film. [13] Manufacturing method for a fluid device with an internal flow path having an aspect ratio (length to diameter) of 10 or more and a cross-section shaped like a circular tube, and a mean arithmetic roughness of an inner wall surface of the flow path of 0.5 µm or less with a standard deviation of 0.1 µm or less, the manufacturing method comprising the following steps: Forming a through hole with a cross-section shaped like a circle in a resin block; and Suction from one end of the through-hole, while vapor of an organic solvent is introduced from the other end. [14] Manufacturing method according to claim 13, wherein the through-hole is formed by machining the resin block. [15] Manufacturing process according to claim 13, wherein acrylic resin is used as the resin forming the resin block; and dichloromethane is used as the organic solvent. [16] Manufacturing process according to claim 13, further comprising: a step of forming a SiO2 film on the inner wall surface of the through-hole after the step of introducing the vapor of the organic solvent into the through-hole.

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