METHOD FOR THE PREPARATION OF PHARMACEUTICAL COMPOSITIONS WITH IMMUNOGENIC CHIKUNGUNYA VIRUS CHIKV-DELTA5NSP3
Patent Information
- Application Number
- DE602018091948
- Authority / Receiving Office
- DE · DE
- Patent Type
- Patents
- Current Assignee / Owner
- Priority Date
- 2017-09-21
- Filing Date
- 2018-09-19
- Publication Date
- 2026-06-24
- Estimated Expiration
- 2038-09-19
AI Technical Summary
Current methods for producing live attenuated Chikungunya virus (CHIKV-Δ5nsP3) vaccines face challenges in maintaining immunogenicity due to point mutations that reduce vaccine efficacy during adaptation to Vero cells, leading to high sequence heterogeneity and reduced immunogenicity, which complicates industrial-scale production.
A controlled process is developed to minimize immunogenicity-reducing mutations, particularly in the E2 protein, by optimizing cell culture conditions such as low multiplicity of infection (MOI) and reduced passage numbers, ensuring high production yields of immunogenic CHIKV-Δ5nsP3 particles.
The process maintains high immunogenicity and production titers of CHIKV-Δ5nsP3, enabling a stable, safe, and effective one-shot vaccine suitable for industrial application, with controlled immunogenicity-reducing mutations minimized.