Novel nanopore protein mutants of hfab and their use in sequencing
Novel nanoporin mutants with targeted mutations and modifications address the limitations of existing nanopore sequencing by enhancing pore stability and accuracy, providing improved sequencing signals and reduced noise.
HK40135015APending Publication Date: 2026-07-17BEIJING POLYSEQ BIOTECH CO LTD +1
Patent Information
- Authority / Receiving Office
- HK · HK
- Patent Type
- Applications
- Current Assignee / Owner
- BEIJING POLYSEQ BIOTECH CO LTD
- Filing Date
- 2026-05-14
- Publication Date
- 2026-07-17
AI Technical Summary
Technical Problem
Current nanopore sequencing technologies face challenges with scarce suitable nanoporins, poor sequencing signals, and low accuracy due to inadequate pore stability, key amino acid composition, and pore diameter range in nanopore walls.
Method used
Development of novel nanoporin mutants with specific mutations and modifications at critical positions, including deletions and additions of amino acids, to enhance pore stability and sequencing accuracy, using recombinant expression vectors in host cells like Escherichia coli.
Benefits of technology
The novel nanoporin mutants exhibit improved sequencing current properties, increased signal amplitude, reduced spontaneous blockage, and enhanced accuracy, offering more options for nanopore sequencing with stable pore currents.
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Abstract
The invention relates to a novel HfaB nanopore protein mutant and application thereof in sequencing, and belongs to the technical field of nanopores. The polynucleotide for coding the nanopore protein mutant and the recombinant expression vector can express the nanopore protein mutant, and can be used for constructing recombinant cells or recombinant strains for expressing the nanopore protein mutant. The wild-type nanopore protein provided by the invention has relatively high pore stability and is suitable for nanopore sequencing, the sequencing current property of the nanopore protein mutant obtained by modification on the basis of the wild-type nanopore protein is further improved, the sequencing precision is obviously improved, the sequencing signal amplitude and step number are obviously increased, the pore current stability is enhanced, and the nanopore protein mutant can be applied to nanopore sequencing. The spontaneous blocking condition is reduced, and the abnormal noise on the via hole signal is reduced.
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