Plant growth-promoting composition

Abstract

To provide a plant growth-promoting composition that is capable of promoting plant growth with greater safety and reduced cost compared to conventional materials.SOLUTION: A plant growth-promoting composition contains, as an active ingredient, at least one selected from the group consisting of onion extracts and onion powder.SELECTED DRAWING: None

Description

[Technical field]

[0001] The present invention relates to a plant growth promoting composition and a method for promoting plant growth. [Background technology]

[0002] Currently, due to the explosive growth of the human population, it is becoming increasingly difficult to produce sufficient quantities of food plants. It is a global issue to continue to promote the development of green spaces. In addition, the rise in earth's surface temperature due to global warming is also a problem. As a result, environmental problems in growing plants are also increasing. Excessive stress due to environmental factors often causes problems in plant growth.

[0003] Therefore, there is a demand for technology that can promise more efficient harvesting than conventional methods. Mulant is a plant nutrient that regulates abiotic stresses to plants, helping them adapt to climate and soil conditions. This technology is attracting attention as a new technique that reduces damage to plants caused by irritation and provides healthy plants. It is being watched.

[0004] Patent Document 1 discloses a plant stomatal opening promoter containing stevia. By artificially promoting the opening of plant stomata using Tevia, it is possible to improve heat stress tolerance. It has been reported that: Patent Document 2 discloses a method for producing a yeast cell wall decomposition product containing ethylene and / or jasmonic acid sigma. The present invention discloses a composition for activating a gene that activates the null transduction system, and the composition is a composition for activating a gene that activates the null transduction system in yeast cell walls. By feeding the decomposition products to plants, the plant defenses involved in the antibacterial properties and disease prevention of the plant body can be obtained. It has been reported that it activates the expression of the syn gene. Patent Document 3 describes a method for inoculating a cell wall-containing substance of a methanol-utilizing bacterium into a plant for cultivating grains. This discloses a method for increasing the yield of grains by [Prior art documents] [Patent documents]

[0005] [Patent Document 1] Patent No. 6741262 [Patent Document 2] Patent No. 4931388 [Patent Document 3] Patent Publication No. 2019-180361 Summary of the Invention [Problem to be solved by the invention]

[0006] As mentioned above, various materials have been used to improve abiotic stress tolerance and increase yield in plants. There have been studies on the use of onion extract or onion powder. has not been reported. [Means for solving the problem]

[0007] As a result of extensive research, the present inventors have developed a highly accurate screening method. Among other ingredients, the onion extract and onion powder are selected from the group consisting of It has been found that one or more of these can be used as an active ingredient for promoting plant growth, and the present invention has been completed.

[0008] That is, the present invention is as follows. [1] The product contains one or more active ingredients selected from the group consisting of onion extract and onion powder. A plant growth promoting composition comprising: [2] Elongation of stems, leaves or roots, increase in the number of leaves, promotion of flowering or fruiting, flower or fruit growth From the group consisting of increasing the number of plants, increasing plant weight or crop yield, greening, and promoting tillering. The plant growth-promoting composition according to the above-mentioned [1], which has one or more selected plant growth-promoting effects. . [3] The plant growth promoting agent according to the above-mentioned [1] or [2], which has an effect of improving abiotic stress resistance. composition. [4] Temperature stress, chemical stress, light stress, drought stress, pH stress, salt stress stress, hypoxic stress, herbivore stress, physical stress, disease stress The plant growth promoting agent according to the above [3], which has an effect of improving stress resistance to one or more of the above-mentioned composition. [5] The plant growth-promoting composition according to [3] above, which activates genes in the stress response system. [6] The product contains one or more active ingredients selected from the group consisting of onion extract and onion powder. A plant growth promoting composition comprising the above compound is used in a plant or in soil or culture solution in which the plant grows. Method for promoting plant growth. [7] The amount of the plant growth promoting composition used in the culture solution is 0.001 to 1% by mass. [6] The method for promoting plant growth described above. Effect of the Invention

[0009] Contains one or more members selected from the group consisting of the onion extract and onion powder of the present invention By applying this composition to plants, plant growth can be promoted more safely and inexpensively than with conventional materials. It can be promoted. [Brief description of the drawings]

[0010] [Figure 1]FIG. 1 shows images showing improvement in root establishment of rice plants when no onion extract or onion powder was added and when 0.36 g (Example 1) of the plant growth-promoting composition containing onion powder was added. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0011] The following describes in detail the embodiment of the present invention (hereinafter, also referred to as the "present embodiment"). The present invention is not limited to the present embodiment, but may be modified within the scope of the present invention. The above-mentioned embodiment can be modified in various ways.

[0012] <Plant growth promoting composition> The plant growth promoting composition in this embodiment comprises onion extract and onion powder. Onion contains one or more active ingredients selected from the group consisting of Amaryllidaceae. It is a plant of the Allium genus (Allium lidaceae). Specifically, Allium cepa is one example. The variety and origin of the onion are not particularly limited, and examples thereof include yellow onions, white onions, and purple onions. (Red onion), salad onion, pearl onion, rubi Onions, green onions, shallots, etc., more specifically, Kita-Wase No. 3, SN-1, SN-3, SN-3A, Okhotsk 222, Okhotsk No. 1, Okhotsk 333, Kitamomiji 2000, Super Kitamomiji, H235, Kitahayate 2, Bullet Bear, Arrow Bear, Power Wolf, Brown Bear, Kodiak, Ikol, Vegas, Hayajiro, Sarari, Caloe One, Ezomaru, Sarasa Gold, Quer Gold, Kurenai, R-5, Red Eye Early, Red Eye II, Red Eye, Red One, Yukigeisha 2, Yukigeisha, White No. 1, Kita-Wase No. 1, Pekoros 22 Super, Pekoros F Okhotsk, Kitami Yellow, Sapporo Yellow, Sky Chihuang, OPP-5, OMP-3, OSP-3, CS3-12, Toyohira, Shippo Wase No. 7, Sonic, Tarzan, Turbo, Uzushio, Advance, Answer, Neo Earth, Power, Examples of varieties include Keru Tama and Momiji.

[0013] In the present embodiment, the onion extract is a concentrated product obtained by extracting specific components from onions. The part used for extraction is not particularly limited, and examples thereof include leaf blades, leaf sheaths (petioles), bulbs (scales), etc. These include leaves, storage leaves, edible parts), bark (protective leaves), core, stem (flower stalk, flower head bolt), and roots. Onion extract may be used alone or in combination. The onion powder can be obtained by the method described in the section entitled "Preparation method of the composition." Onions or onion extract are dried and crushed, but are not particularly limited. .

[0014] The plant growth promoting composition in this embodiment is a composition having a plant growth promoting effect, Here, the plant growth promoting effect refers to, for example, the elongation of stems, leaves or roots, the increase in the number of leaves, the flowering or promotion of fruit set, increase in the number of flowers or fruits, increase in plant weight or crop yield, Examples of the effects include promoting growth, such as greening or promoting tillering.

[0015] More specifically, the plant growth promoting effect is observed when, for example, the plant growth promoting effect of the present embodiment is observed. The actual weight increase (%) or The increase in dry weight (%) in the plant growth promoting composition is called the increase in dry weight (%) in the plant growth promoting composition. The actual weight increase (%) or dry weight increase (%) of the plant growth promoting composition of this embodiment is It is preferably 10% or more, more preferably 20% or more, and even more preferably 10% or more, compared to when no substance is used. The plant growth promoting effect is when the plant growth promoting compound is present in the plant. The use of a plant growth promoting composition increases the growth of plants in areas where no growth is observed without the use of the composition. The above figures include those in which the plant growth-promoting composition is applied. This value is the average value for the entire plant.

[0016] In addition, the plant growth promoting effect also includes the effect of improving the activity of plants, such as: do. 1) Improved root growth and root survival rate 2) Increased efficiency of nutrient absorption 3) Induction of flower buds, promotion of flowering, and increase in fruit production 4) Accumulation of sugar and other substances 5) Increased water saving effect 6) Callus induction effect 7) Increase in plant size, especially elongation or thickening of above-ground and below-ground parts 8) Repairing scratches That is, the plant growth-promoting composition of the present embodiment promotes the elongation of stems, leaves or roots, and an increase in the number of leaves. Increase in the number of flowers or fruits, plant weight or crop yield In addition to the growth promoting effects such as increased vegetation, greening, and promotion of tillering, the effects of 1) to 8) above can be achieved. It may have such an action.

[0017] The plant growth promoting composition of the present embodiment has an effect of improving abiotic stress resistance. In other words, it is possible to improve tolerance to various abiotic (environmental) stresses that plants are exposed to. Here, examples of abiotic stress include high temperature stress. stress, temperature stress such as low temperature stress, chemical stress, light stress, drought stress, oxidation stress, etc. Stress, such as pH stress, osmotic stress, salt stress, hypoxic stress, feeding stress, etc. These include stress from plants, physical stress, and disease stress.

[0018] In this embodiment, "abiotic stress tolerance" refers to the ability to tolerate environmental stress under growth conditions. Even if the plant is not viable, it may die or grow poorly (e.g., whitening or yellowing of the plant body, shortening of root length, etc.). reduction in plant weight or crop yield), reduced growth rate, or The term "characteristics" refers to a trait that allows a plant to grow normally without being substantially affected by such undesirable effects. The plant growth promoting composition of this embodiment improves the abiotic stress tolerance of plants. This allows plants to grow properly even in external environments that are not favorable for normal plant growth. It can always be grown.

[0019] The effect of imparting temperature stress resistance to the plant growth-promoting composition in this embodiment is, for example, It has the effect of promoting normal growth under high temperature conditions of 35℃ to 55℃ and low temperature conditions of 0℃ to 10℃. In addition, the salt stress-inducing effect is shown by the addition of 0.1 to 3.0 mass% sodium chloride. The effect of imparting drought stress tolerance is achieved by increasing the relative humidity. Normal growth is possible even at 10-40%.

[0020] In this embodiment, the abiotic stress tolerance of a plant can be evaluated, for example, by the following means: The salt stress tolerance can be evaluated by adding salt to the target plant at an appropriate concentration. The plant is grown in a medium or soil containing the above-mentioned ingredients under conditions of temperature and duration suitable for the plant. The presence or absence of drought stress tolerance can be confirmed by culturing the plant and evaluating its phenotype. The method is to grow the target plant under conditions of dry growth temperature, growth humidity, and growth period for the plant. The phenotype can be evaluated by growing the strain at low temperature. The presence or absence of loess tolerance was determined by first acclimating the target plant to a low temperature that was not freezing, and then The plant is grown under conditions of optimum temperature and period, and its phenotype is evaluated. This can be confirmed by:

[0021] When the plant growth promoting composition of this embodiment has an abiotic stress tolerance improving effect Alternatively, the present invention may be one that activates a gene in a stress response system. There is no particular limitation on the type of response indicator, and it is generally known as a response indicator to temperature stress or drought stress. Examples of genes that have been identified include:

[0022] The plant growth promoting composition in this embodiment contains onion extract or onion powder alone. It may comprise both onion extract and onion powder, and may comprise one or more agricultural There are no particular limitations on the agriculturally acceptable ingredients. Examples of suitable substances include, but are not limited to, solvents, carriers, excipients, binders, solubilizers, stabilizers, enhancers, etc. Viscosity agents, swelling agents, lubricants, surfactants, oily liquids, buffers, disinfectants, antifreeze agents, defoamers, colorants , antioxidants, and further active ingredients. These include water, mineral oil fractions such as kerosene or diesel, and oils of vegetable or animal origin. Oils, cyclic or aromatic hydrocarbons (e.g., paraffins, tetrahydronaphthalene, alkyl alkylated naphthalenes or their derivatives, or alkylated benzenes or their derivatives derivatives), alcohols (e.g. methanol, ethanol, propanol, butanol or cyclohexanol), ketones (e.g., cyclohexanone), or amines (e.g., N Agriculturally acceptable liquid carriers such as ethyl 2-methylpyrrolidone, ethyl 2-methylpyrrolidone, or mixtures thereof are preferred. stomach.

[0023] When the plant growth promoting composition of this embodiment contains one or more additional active ingredients Further, the active ingredient may be any active ingredient known in the art that has an activity of improving resistance to various environmental stresses. The plant growth promoting composition may be applied with one or more of such further compounds. By containing active ingredients that can improve the abiotic stress tolerance of plants, There is a tendency for this to be possible.

[0024] The plant growth promoting composition of the present embodiment is used in combination with conventionally known agricultural materials such as pesticides and fertilizers. As agricultural materials, they can be used with bacteria such as actinomycetes and microbial components such as yeast. It is preferred to use

[0025] The plant growth promoting composition of the present embodiment is used as an agricultural chemical formulation or a pesticide to promote plant growth. The plant growth promoting composition can be used, for example, in the form of a solid (e.g. In any form, such as powder or granules, liquid (e.g. solution or suspension) It is preferable to use the compound in a liquid form such as a solution or suspension. In this case, the composition may be in a liquid state, and may be used as a liquid prepared just before application. .

[0026] The formulation of the plant growth promoting composition of the present embodiment is not particularly limited, and may be any formulation commonly used in the art. The drug may be formulated in the form of an emulsifiable concentrate, wettable powder, liquid, water-soluble powder, dust, powder, paste, or granules. It is possible.

[0027] In this embodiment, the plant to which the plant growth promoting composition is applied is not particularly limited. Plants of interest include, for example, angiosperms and gymnosperms. For example, Asteraceae plants such as chrysanthemums and gerberas, potatoes, tomatoes and eggplants such as aubergines. Brassica plants such as rapeseed and canola, rice, corn, wheat, sorghum, Gramineous plants such as corn and barley, legumes such as soybeans, and carrots plants of the parsley family, such as basil, mint and rosemary, plants of the lamiaceae family, such as morning glory Convolvulaceae, Salicaceae such as poplar, castor, cassava and jatropha Euphorbiaceae such as the sweet potato; Convolvulaceae such as oranges and lemons; citrus plants such as citrus, rose plants such as cherry blossoms and roses, orchids such as moth orchids Gentianaceae plants such as bellflowers, Primulaceae plants such as cyclamen , violets like pansies, lilies like lilies, and amaranths like sugar beets. plants of the Vitaceae family such as grapes, plants of the Cupressaceae family such as cedars and cypresses, olives and cypresses Examples of such plants include plants of the Oleaceae family, such as Osmanthus grandiflorus, and plants of the Pinaceae family, such as Pinus densiflora.

[0028] The target plants include not only whole plants (i.e. complete plant bodies) but also groups of plants. Tissues or organs (e.g. cut flowers or vegetative propagation such as rhizomes, tubers, corms or runners) The plant may be a part of a plant, such as an organ, cultured cell and / or callus.

[0029] The plant growth promoting composition in this embodiment is used for any growth of a plant, including before or after germination. The whole or part of a plant at any stage (e.g., a seed, a seedling, or the whole or part of a mature plant) It can be applied for 10 minutes.

[0030] <Method for preparing plant growth promoting composition> The method for preparing the plant growth promoting composition includes, for example, After obtaining the above, it can be mixed with the above-mentioned agriculturally acceptable ingredients as necessary to obtain the above-mentioned composition. can. There is no particular restriction on the method for obtaining onion extract, and it can be obtained using any method for extracting general plant extracts. Specifically, for example, after crushing the bulbs of onions, the impurities are removed by and concentrating the onion juice obtained in the above step. obtaining an onion concentrate by fractionating the onion concentrate obtained in the above step, It can be obtained by a method including a step of obtaining an onion extract containing specific components. In the process of obtaining onion extract, hot water extraction and solvent extraction using various solvents are used. For hot water extraction, for example, onions are chopped and then cooled. One method is to add water to onions and extract the soluble components at 60 to 100°C. For example, onions are heated at temperatures between -15°C and -25°C until the cell membrane is destroyed. For example, the method includes treating the mixture for a certain period of time, followed by extraction with water or an alcohol-based solvent. The method for obtaining onion powder is not particularly limited, and may be fresh or frozen onions or onion extract. After drying the material using methods such as hot air drying, air drying, dry heat drying, and vacuum drying, it is crushed into powder form. It can be obtained by

[0031] (Crushing and removal process) Crushing of onions is carried out using fresh or dried onions, for example in a mixer. When crushing onions, use an appropriate amount of water to facilitate processing in a mixer, etc. Water may be added. The crushed onion is filtered through a filter cloth or paper to remove impurities. The onion juice is obtained from which the fermentation products have been removed. When filtering, an extraction solvent such as water or alcohol is not used. Alternatively, instead of filtration, the supernatant may be separated by centrifugation. You may remove things.

[0032] (concentration process) The obtained onion juice is concentrated using a rotary evaporator or the like to produce onion juice. A concentrated solution can be obtained. Concentration may be performed using a centrifugal evaporator or by freeze-drying. The concentration ratio is preferably 3 to 30 times, more preferably 4 to 20 times. If the concentration rate is 5 times or more, the amount of the plant growth promoting composition added to the plant is reduced. The handling property improves and the transportation cost tends to be reduced. If it is less than 30 times, the plant There tends to be less sediment in the growth promoting composition making it easier to handle. In the preparation of the plant growth-promoting composition, it is not essential to concentrate the onion juice. The onion juice thus obtained may be subjected as it is to the following fractionation step.

[0033] (Fractionation process) The onion concentrate obtained is fractionated through a column to isolate onions containing specific components. In the fractionation step, for example, a hydrophilic fraction is separated by passing through a column. From these, the hydrophilic fraction is preferably used as the onion extract. In addition, the onion concentrate is preferably autoclaved or otherwise processed before fractionation using a column or the like. The heat treatment may be carried out by

[0034] The onion extract obtained in the fractionation process can be further purified or highly active fractions separated as required. The purification process may be, for example, filtration, adsorption (ion exchange resin column, The highly active fraction can be separated by gel electrophoresis. Examples of such treatments include filter filtration, adsorption treatment, silica gel column chromatography, and HPLC. do.

[0035] <Plant growth promotion method> The plant growth promoting method of the present embodiment is a method for producing a plant growth promoting agent comprising the steps of: A composition containing one or more active ingredients selected from the group consisting of This method promotes plant growth by applying it to the soil or culture solution in which the plant grows. In the method, the plant growth promoting composition is applied to plants at any growth stage, including pre-emergence or post-emergence. It can be applied to plants or parts thereof (e.g., seeds, seedlings or mature plants). It can be applied not only to the plant itself, but also to the soil, medium or culture solution in which the plant grows. A plant growth promoting composition is added to a plant in the growth stage or to the soil, medium or culture solution in which the plant is grown. By applying the substance, the growth of plants can be promoted.

[0036] The plant growth promoting composition may be applied to the plant once or multiple times. In addition, when the application period is multiple, the number of applications at each application period and The above may be performed once or multiple times.

[0037] The plant growth promoting composition can be applied to plants by, for example, adding, spraying, coating, or immersing. These may be used alone or in combination of two or more. This is also fine.

[0038] The amount of the plant growth promoting composition applied to a plant is determined based on the amount of the plant growth promoting composition applied to the culture solution in which the plant grows. In this case, the amount of the plant growth-promoting composition used in the culture solution is preferably 0.001 to 1% by mass. When the amount of the plant growth promoting composition used is within the above range, a sufficient plant growth promoting effect can be obtained. There is a tendency for

[0039] The preferred range of the amount used varies depending on the concentration of onion juice described above. However, the above-mentioned amount used is a suitable range when the concentration rate is 3 to 30 times. EXAMPLES

[0040] The present invention will be described in more detail with reference to examples and comparative examples. The present invention is not limited in any way by the above.

[0041] (Method for measuring root weight) The roots of the rice plants were identified based on their color and shape, and the weight of the identified roots was measured using a microbalance.

[0042] [Example 1] Slice 246.5g of onion bulbs into 1-10mm slices and add 100g of water. The mixture was added and crushed in a mixer. Impurities were removed by filtration using filter cloth and filter paper. The filtrate was concentrated about 10 times using a rotary evaporator to obtain an onion extract. The solution containing the fraction obtained by fractionating and heat-treating the onion extract obtained above was then applied to starved rice plants. 0.36 g (0.04 mass%) was added to 900 mL of growing culture solution, and the effect on rice was examined. The results showed that the weight of the rice roots was This was an increase of 58.5%, demonstrating the effect of improving the root establishment of rice (see Figure 1). In this case, starvation refers to a state in which the nutritional content is below that shown in Table 1 below. .

[0043] [Table 1]

Claims

1. The onion extract and onion powder are the active ingredients. A plant growth promoting composition comprising:

2. Elongation of stems, leaves or roots, increase in the number of leaves, promotion of flowering or fruiting, flower or fruit growth From the group consisting of increasing the number of plants, increasing plant weight or crop yield, greening, and promoting tillering. The plant growth-promoting composition according to claim 1, which has one or more selected plant growth-promoting effects.

3. The plant growth promoting composition according to claim 1 or 2, which has an effect of improving abiotic stress resistance. 。

4. Temperature stress, chemical stress, light stress, drought stress, pH stress, salt stress stress, hypoxic stress, herbivore stress, physical stress, disease stress The plant growth promoting composition according to claim 3, which has an effect of improving stress resistance to one or more of the above-mentioned compounds. Composition.

5. The plant growth promoting composition according to claim 3, which activates a gene in a stress response system.

6. The onion extract and onion powder are the active ingredients. A plant growth promoting composition comprising the above compound is used in a plant or in soil or culture solution in which the plant grows. Method for promoting plant growth.

7. The amount of the plant growth promoting composition used in the culture solution is 0.001 to 1% by mass. Item 7. The plant growth promoting method according to Item 6.

8. Onion extract or onion powder is made from onion waste from farms, grading houses or factories.

7. The onion extract or onion powder according to claim 6, which is extracted from one or more parts of the green onion. A method for promoting plant growth.

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