Formulations of PSMA imaging agents

Abstract

To provide formulations of radiolabeled compounds for use in radiotherapy and diagnostic imaging related to prostate specific membrane antigen (PSMA).SOLUTION: The present invention relates to formulations of radiolabeled compounds for use in radiotherapy and diagnostic imaging related to prostate specific membrane antigen (PSMA).SELECTED DRAWING: None

Description

[Technical field]

[0001] The present invention relates to the formulation of radiolabeled compounds for use in radiotherapy and diagnostic imaging involving prostate-specific membrane antigen (PSMA). [Background technology]

[0002] Prostate cancer is the leading cause of cancer-related deaths in men, with mortality often due to the difficulty in detecting and subsequently treating the disease. Prostate-associated tumors often show increased prostate-specific membrane antigen (PSMA) expression. PSMA is an enzyme that is normally expressed in prostate tissue, but is often increased in some prostate cancers. This means that PSMA is an excellent biomarker or target for imaging, diagnostic, and prognostic purposes. However, PSMA is also expressed in other tissues, both normal and malignant, making successful imaging of prostate cancer difficult. Radiolabeled complexes can be used for imaging and treatment of cancers such as prostate cancer, but some complexes containing radioisotopes or radionuclides and targeting ligands may be unstable and prone to dissociation.If the formed complex is not strong enough, dissociation may occur immediately after formation, i.e., during the radiolabeling process.Although radiolabeling processes are known, in these processes, the complex may not be formed in sufficient yield or the entire complex solution may not be radiochemically pure.In addition, even if radiolabeled complexes can be produced, purification and isolation procedures that allow the complete complex to be obtained in good yield are preferred.

[0003] Even if a radiolabeled complex is obtained, the complex may be unstable and prone to decomposition. This may result in dissociation of the radioisotope, reduced radiochemical yield and purity of the complex-containing preparation, and limited efficiency of the preparation. If the radioisotope is lost and not delivered to the desired cancer site, imaging and / or therapy will be either poor or insufficient. Radiolabeled complexes may also be susceptible to radiolysis, where the activity of the radioisotope leads to the destruction and decomposition of the ligand, due to spontaneous decay of the radioisotope. This leads to the release of the radioisotope. Diffusion of free radioisotope to other areas as a result of the circulatory system may result in the delivery of radioactivity to locations where delivery is not desired. There is a need for stable formulations of radiolabeled complexes suitable for imaging and treating prostate cancer. There is also a need for effective methods for preparing such stable formulations. Summary of the Invention

[0004] In one aspect of the invention, there is provided an aqueous formulation for parenteral administration comprising a compound of formula (I) or a salt thereof complexed with Cu ions, the formulation further comprising at least one of gentisic acid, ascorbic acid, L-methionine, pyridoxine, or salts thereof. [ka] Formula (I)

[0005] In a further aspect, there is provided an aqueous formulation for parenteral administration comprising a compound of formula (Ia) or a salt thereof complexed with Cu ions, the formulation further comprising at least one of gentisic acid, ascorbic acid, L-methionine, pyridoxine, or salts thereof. [ka] Formula (Ia)

[0006] In another aspect of the present invention, there is provided an aqueous formulation for parenteral administration comprising a compound of formula (I) or a salt thereof complexed with Cu ions, the formulation further comprising a buffer solution. [ka] Formula (I)

[0007] In a further embodiment, there is provided an aqueous formulation for parenteral administration comprising a compound of formula (Ia) or a salt thereof complexed with Cu ions, the formulation further comprising a buffer solution. [ka] Formula (Ia)

[0008] In one embodiment, the aqueous formulation comprises gentisic acid or a salt thereof. In another embodiment, the aqueous formulation comprises ascorbic acid or a salt thereof. In another embodiment, the aqueous formulation comprises L-methionine or a salt thereof. In another embodiment of the present invention, there is provided a method for preparing a formulation comprising a compound of formula (I) complexed with a Cu radioisotope, comprising the steps of: i) adding an amount of a compound of formula (I) to an acetate buffer; ii) adding a solution of Cu radioisotope in hydrochloric acid to the compound of formula (I) and acetate buffer; and iii) heating the mixture of step ii) for a time and under conditions to form a complex of formula (I) with the Cu radioisotope. A method is provided, comprising:

[0009] [ka] Formula (I)

[0010] In another embodiment of the present invention, there is provided a method for preparing a formulation comprising a compound of formula (I) complexed with a Cu radioisotope, comprising the steps of: i) adding an amount of a compound of formula (I) to a phosphate buffer; ii) adding a solution of Cu radioisotope in hydrochloric acid to the compound of formula (I) and phosphate buffer; and iii) reacting the mixture of step ii) for a time and under conditions to form a complex of formula (I) with the Cu radioisotope. A method is provided, comprising: [ka] Formula (I)

[0011] In an embodiment of the process for preparing a complex defined herein, the compound of formula (I) has the structure of a compound of formula (Ia). [ka] Formula (Ia) In one embodiment, the Cu radioisotope is 61 It is Cu. In one embodiment, the Cu radioisotope is 64 It is Cu. In another embodiment, the Cu radioisotope is 67 It is Cu. In a further embodiment, the pH of the formulation is maintained in the range of between about 4 and about 8. In another embodiment of the present invention, there is provided a method for purifying a compound of formula (I) complexed with a Cu radioisotope, comprising the steps of: i) loading a solution of compound of formula (I) complexed with a Cu radioisotope into a solid phase extraction cartridge; ii) eluting the compound of formula (I) complexed with the Cu radioisotope using an eluent containing water, ethanol and sodium chloride. A method is provided, comprising:

[0012] [ka] Formula (I)

[0013] In an embodiment of the method of purifying a complex defined herein, the compound of formula (I) has the structure of a compound of formula (Ia): [ka] Formula (Ia) In one embodiment, the purified compound of formula (I) or a salt thereof complexed with a Cu radioisotope, purified according to the previous aspect, is prepared according to another aspect of the invention. In one embodiment, a compound of formula (I) or a salt thereof complexed with a Cu radioisotope is prepared according to another aspect of the invention. [Brief description of the drawings]

[0014] [Figure 1] 1 is a graph of the radiochemical purity of purified Formula (Ia) and 64Cu complex solutions that contained either gentisic acid, ascorbic acid, or L-methionine and were monitored over a 48 hour period. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0015] Formulation of the complex of formula (I) The present invention relates to stable formulations of certain radioisotope-ligand complexes. The inventors have found that formulations of the complexes disclosed herein minimize dissociation of the radioisotope from the ligand and / or minimize radiolysis of the ligand due to the radioisotope. The formulations of radioisotope-ligand complexes referred to herein are stable for some time in solution and under physiological conditions. The stability of the formulation is related to the stability of the complex. The radioisotope may dissociate from the complex, which results in less radioactivity being delivered to the site where the ligand is attached. This energy, when released, may lead to the decomposition of the ligand, called radiolysis, since the radioisotope undergoes spontaneous decay or release of energy. The radiostability of the complex can be measured by considering the radiochemical purity of the formulation. Radiochemical purity is defined as the amount of radioisotope complexed with the sarcofazine ligand, and is expressed as a percentage of the total amount of radioisotope present in the formulation. The radioisotope may be present in the formulation as a complex with the sarcofazine ligand, as a free radioisotope, or as part of a radiolysis product.

[0016] Ligands containing urea-based motifs have been found to bind to the catalytic site of prostate-specific membrane antigen (PSMA), which is normally expressed in prostate tissue and is increased in some prostate cancers.An example of a ligand containing such a motif is the macrocyclic ligand 1,8-diamino-3,6,10,13,16,19-hexazabicyclo[6.6.6]icosane (also known as sarcophagine or "Sar"), Sar-bisPSMA.In this compound, each terminal amine group is linked to a linker group and a urea-based motif. Sar-bisPSMA is shown in formula (I). [ka] Formula (I) The compound of formula (I) may be produced by a series of coupling reactions between the sarcofazine ligand, the linker, and the urea motif. Procedures for the preparation of the compound of formula (I) can be found in WO2018 / 223180.

[0017] Compounds of formula (I) may have the structure of formula (Ia), as shown below, where the stereochemistry of the compound is determined. [ka] Formula (Ia) Unless otherwise specified, any reference below to a compound of formula (I) should be taken to also include a reference to a compound of formula (Ia).

[0018] The present invention relates to the use of compounds of formula (I) and (Ia) in formulations. Compounds of formula (I) and (Ia) can be used as pharma- ceutically acceptable salts. Compounds of formula (I) and (Ia) contain two urea motifs that can bind independently to the catalytic site of PSMA. The inventors believe that the increased binding affinity of the compound of formula (I) at the desired site is due to the presence of the second urea motif. Without wishing to be bound by theory, the inventors believe that the additional binding affinity of the compound of formula (I), which appears to be more effective than the use of twice the amount of an analogous compound with only one urea motif, is related to the presence of the second urea motif. Subsequently, the formulations described herein containing compounds of formula (I) or (Ia) show superior efficacy to formulations of analogous compounds containing urea motifs.

[0019] The term "pharmaceutically acceptable salt" refers to a salt that retains the desired biological activity of the above-identified compounds, including pharmaceutically acceptable acid addition salts and base addition salts. Suitable pharmaceutically acceptable acid addition salts of the compounds of formula (I) and (Ia) may be prepared from inorganic or organic acids. Examples of such inorganic acids are hydrochloric acid, sulfuric acid, and phosphoric acid. Suitable organic acids can be selected from the classes of organic acids of aliphatic, alicyclic, aromatic, heterocyclic carboxylic and sulfonic acids, examples of which are formic acid, acetic acid, propionic acid, succinic acid, glycolic acid, gluconic acid, lactic acid, malic acid, tartaric acid, citric acid, fumaric acid, maleic acid, alkylsulfonic acid, and arylsulfonic acid. Additional information regarding pharmaceutically acceptable salts can be found in Remington's Pharmaceutical Sciences, 19th Edition, Mack Publishing Co., Easton, PA 1995. For drugs that are solid, it will be understood by those of skill in the art that the compounds, drugs and salts of the invention may exist in various crystalline or polymorphic forms, all of which are intended to be within the scope of the invention and the prescribed formulations.

[0020] In a preferred embodiment, the compound of formula (I) is provided as the acetate salt. The formulations of the present invention comprise a compound of formula (I) or a salt thereof, and a radioisotope. The radioisotope, also called a radionuclide, can be a metal or a metal ion. The compounds of formula (I) herein are capable of ionizing copper ions, particularly Cu 2+ It has been found to be particularly effective in complexing ions. One skilled in the art will appreciate that a complex of a compound of formula (I) can be produced by contacting the compound of formula (I) with a desired radioisotope, where the radioisotope is Cu. 2+ It is an ion. In one embodiment, the ligand is complexed with a Cu ion. The copper ion may be radioactive and thus may be a radionuclide or radioisotope of copper. In one embodiment, the ligand is 60 In another embodiment, the ligand is 61 In another embodiment, the ligand is 64 In another embodiment, the ligand is 67 In a preferred embodiment, the ligand is 64 In another preferred embodiment, the ligand is 67 It forms a complex with Cu. The complex of formula (I) with Cu radioisotope may be unstable and prone to radiolysis in solution. The inventors have found that the solubilized complex may be stabilized when one or more stabilizers are added to the formulation containing the complex. Such stabilizers include gentisic acid, ascorbic acid, L-methionine, pyridoxine and their salts.

[0021] The formulations of the present invention may include at least one of gentisic acid, ascorbic acid, L-methionine and pyridoxine, or salts thereof. The inventors have determined that the addition of gentisic acid, ascorbic acid, L-methionine and / or pyridoxine to the formulations of the present invention helps to prevent or minimize radiolysis of the complex of formula (I), thus improving the radiostability of the complex and its formulations. Gentisic acid is also known as 2,5-dihydroxybenzoic acid, 5-hydroxysalicylic acid or hydroquinone carboxylic acid. Salts of gentisic acid may include sodium salt and sodium salt hydrate. Any reference to gentisic acid may include a reference to its salt, if relevant. Other isomers of dihydroxybenzoic acid are also contemplated. Examples of other isomers include 2,4-dihydroxybenzoic acid and 2,5-dihydroxybenzoic acid, and their salts. In one embodiment, gentisic acid is present in the formulation in an amount of about 0.02% to about 0.1% (w / v). In one embodiment, gentisic acid or a salt thereof is present in the formulation in an amount of about 0.02% (w / v). In another embodiment, gentisic acid or a salt thereof is present in the formulation in an amount of about 0.025% (w / v). In another embodiment, gentisic acid or a salt thereof is present in the formulation in an amount of about 0.03% (w / v). In another embodiment, gentisic acid or a salt thereof is present in the formulation in an amount of about 0.035% (w / v). In another embodiment, gentisic acid or a salt thereof is present in the formulation in an amount of about 0.04% (w / v). In another embodiment, gentisic acid or a salt thereof is present in the formulation in an amount of about 0.045% (w / v). In another embodiment, gentisic acid or a salt thereof is present in the formulation in an amount of about 0.05% (w / v). In another embodiment, gentisic acid or a salt thereof is present in the formulation in an amount of about 0.055% (w / v). In another embodiment, gentisic acid or a salt thereof is present in the formulation in an amount of about 0.6% (w / v). In another embodiment, gentisic acid or a salt thereof is present in the formulation in an amount of about 0.065% (w / v). In another embodiment, gentisic acid or a salt thereof is present in the formulation in an amount of about 0.07% (w / v). In another embodiment, gentisic acid or a salt thereof is present in the formulation in an amount of about 0.075% (w / v). In another embodiment, gentisic acid or a salt thereof is present in the formulation in an amount of about 0.08% (w / v). In another embodiment, gentisic acid or a salt thereof is present in the formulation in an amount of about 0.085% (w / v). In another embodiment, gentisic acid or a salt thereof is present in the formulation in an amount of about 0.09% (w / v). In another embodiment, gentisic acid or its salt is present in the formulation in an amount of about 0.095% (w / v). In another embodiment, gentisic acid or its salt is present in the formulation in an amount of about 0.1% (w / v). In other embodiments, gentisic acid or its salt ranging between the amounts recited above is also contemplated by the present invention. In a preferred embodiment, gentisic acid or its salt is present in the formulation in an amount of 0.056% (w / v) or less.

[0022] L-methionine is an amino acid containing a thiol ether side chain and is also known as Met or L-Met. Salts of L-methionine include sodium salts. Any reference to L-methionine may include a reference to its salts, where relevant. In one embodiment, L-methionine or a salt thereof is present in the formulation in an amount of about 1 mg / mL to about 4 mg / mL. In one embodiment, L-methionine or a salt thereof is present in the formulation in an amount of about 1.0 mg / mL. In another embodiment, L-methionine or a salt thereof is present in the formulation in an amount of about 1.5 mg / mL. In another embodiment, L-methionine or a salt thereof is present in the formulation in an amount of about 2.0 mg / mL. In another embodiment, L-methionine or a salt thereof is present in the formulation in an amount of about 2.5 mg / mL. In another embodiment, L-methionine or a salt thereof is present in the formulation in an amount of about 3.0 mg / mL. In another embodiment, L-methionine or a salt thereof is present in the formulation in an amount of about 3.5 mg / mL. In another embodiment, L-methionine or a salt thereof is present in the formulation in an amount of about 4.0 mg / mL. In other embodiments, the present invention also contemplates L-methionine or a salt thereof ranging between the amounts recited above. In a preferred embodiment, L-methionine is present in the formulation in an amount of about 3 mg / mL.

[0023] Ascorbic acid is also known as 2,3-didehydro-L-threo-hexano-1,4-lactone or vitamin C. Salts of ascorbic acid include sodium ascorbate, potassium ascorbate, calcium ascorbate, and magnesium ascorbate. Any reference to ascorbic acid may include a reference to its salts, where relevant.

[0024] In one embodiment, the ascorbic acid or its salt is present in the formulation in an amount of about 5 mg / mL to about 50 mg / mL. In one embodiment, the ascorbic acid or its salt is present in the formulation in an amount of about 5 mg / mL. In another embodiment, the ascorbic acid or its salt is present in the formulation in an amount of about 6 mg / mL. In another embodiment, the ascorbic acid or its salt is present in the formulation in an amount of about 7 mg / mL. In another embodiment, the ascorbic acid or its salt is present in the formulation in an amount of about 8 mg / mL. In another embodiment, the ascorbic acid or its salt is present in the formulation in an amount of about 9 mg / mL. In another embodiment, the ascorbic acid or its salt is present in the formulation in an amount of about 10 mg / mL. In another embodiment, the ascorbic acid or its salt is present in the formulation in an amount of about 11 mg / mL. In another embodiment, the ascorbic acid or its salt is present in the formulation in an amount of about 12 mg / mL. In another embodiment, the ascorbic acid or its salt is present in the formulation in an amount of about 13 mg / mL. In another embodiment, the ascorbic acid or its salt is present in the formulation in an amount of about 14 mg / mL. In another embodiment, the ascorbic acid or its salt is present in the formulation in an amount of about 15 mg / mL. In another embodiment, the ascorbic acid or its salt is present in the formulation in an amount of about 20 mg / mL. In another embodiment, the ascorbic acid or its salt is present in the formulation in an amount of about 25 mg / mL. In another embodiment, the ascorbic acid or its salt is present in the formulation in an amount of about 30 mg / mL. In another embodiment, the ascorbic acid or its salt is present in the formulation in an amount of about 35 mg / mL. In another embodiment, the ascorbic acid or its salt is present in the formulation in an amount of about 40 mg / mL. In another embodiment, the ascorbic acid or its salt is present in the formulation in an amount of about 45 mg / mL. In another embodiment, the ascorbic acid or its salt is present in the formulation in an amount of about 50 mg / mL. In other embodiments, the present invention also contemplates ascorbic acid or its salt ranging between the amounts recited above. In a preferred embodiment, ascorbic acid is present in the formulation in an amount of about 10 mg / mL.

[0025] Pyridoxine is also known as 4,5-bis(hydroxymethyl)-2-methylpyridin-3-ol or vitamin B6. Salts of pyridoxine may include hydrochloride salts. In one embodiment, pyridoxine or a salt thereof is present in the formulation in an amount of about 5 mg / mL to about 15 mg / mL. In one embodiment, pyridoxine or a salt thereof is present in the formulation in an amount of about 5 mg / mL. In another embodiment, pyridoxine or a salt thereof is present in the formulation in an amount of about 6 mg / mL. In another embodiment, pyridoxine or a salt thereof is present in the formulation in an amount of about 7 mg / mL. In another embodiment, pyridoxine or a salt thereof is present in the formulation in an amount of about 8 mg / mL. In another embodiment, pyridoxine or a salt thereof is present in the formulation in an amount of about 9 mg / mL. In another embodiment, pyridoxine or a salt thereof is present in the formulation in an amount of about 10 mg / mL. In another embodiment, pyridoxine or a salt thereof is present in the formulation in an amount of about 11 mg / mL. In another embodiment, pyridoxine or a salt thereof is present in the formulation in an amount of about 12 mg / mL. In another embodiment, pyridoxine or a salt thereof is present in the formulation in an amount of about 13 mg / mL. In another embodiment, pyridoxine or a salt thereof is present in the formulation in an amount of about 14 mg / mL. In another embodiment, pyridoxine or a salt thereof is present in the formulation in an amount of about 15 mg / mL. In a preferred embodiment, pyridoxine is present in the formulation in an amount of about 10 mg / mL.

[0026] The formulation of the present invention may contain ethanol as a component. The ethanol used in the formulation may be absolute ethanol. Alternatively, the ethanol used in the formulation may not have been subjected to a drying process and may be hydrated. The ethanol is preferably pharmaceutical grade ethanol. The ethanol present in the formulation may further help prevent the radiolysis of the radiolabeled complex of formula (I).

[0027] In one embodiment, ethanol is present in the formulation in an amount of about 7% to about 13% (v / v). In one embodiment, ethanol is present in the formulation in an amount of about 7% (v / v). In another embodiment, ethanol is present in the formulation in an amount of about 8% (v / v). In another embodiment, ethanol is present in the formulation in an amount of about 9% (v / v). In another embodiment, ethanol is present in the formulation in an amount of about 10% (v / v). In another embodiment, ethanol is present in the formulation in an amount of about 11% (v / v). In another embodiment, ethanol is present in the formulation in an amount of about 12% (v / v). In another embodiment, ethanol is present in the formulation in an amount of about 13% (v / v). In a preferred embodiment, ethanol is present in the formulation in an amount of about 10% (v / v). In other embodiments, the present invention contemplates ethanol ranging between the amounts recited above.

[0028] The formulation of the present invention may also include sodium chloride as a component. The sodium chloride in the formulation of the present invention may be provided as saline. Saline is defined as an aqueous solution of sodium chloride. For example, normal saline is defined as an aqueous solution of sodium chloride with a concentration of 0.9% (w / v). In one embodiment of the present invention, the sodium chloride in the formulation is provided by saline. In one embodiment, sodium chloride is present in the formulation in an amount of about 0.6% to 1.2% (w / v). In one embodiment, sodium chloride is present in an amount of about 0.6% (w / v). In another embodiment, sodium chloride is present in an amount of about 0.7% (w / v). In another embodiment, sodium chloride is present in an amount of about 0.8% (w / v). In another embodiment, sodium chloride is present in an amount of about 0.9% (w / v). In another embodiment, sodium chloride is present in an amount of about 1.0% (w / v). In another embodiment, sodium chloride is present in an amount of about 1.1% (w / v). In another embodiment, sodium chloride is present in an amount of about 1.2% (w / v). In a preferred embodiment, sodium chloride is present in the formulation in an amount of about 0.9% (w / v). In other embodiments, sodium chloride ranging between the amounts recited above is also contemplated by the present invention.

[0029] The formulations of the present invention have a pH of about 4 to about 8. One skilled in the art will appreciate that the pH of a formulation is an inherent property of the formulation resulting from the combination of the compound of formula (I) or its complex with the remaining excipients of the formulation. Alternatively, the pH of the formulation may be altered to a desired value by the addition of one or more buffering agents. Examples of suitable buffer solutions include acetate buffers, which may include a mixture of sodium acetate and acetic acid. In certain embodiments, the formulations of the present invention include acetate buffers. Another suitable buffer solution includes phosphate buffers, which may include a mixture of various phosphate salts or hydrates thereof. Examples of suitable phosphate salts include sodium dihydrogen phosphate (NaH2PO4), disodium hydrogen phosphate (Na2HPO4), potassium dihydrogen phosphate (KH2PO4) and dipotassium hydrogen phosphate (K2HPO4). In one embodiment, the phosphate buffer contains a sodium phosphate salt. In another embodiment, the phosphate buffer contains a potassium phosphate salt. In another embodiment, the phosphate buffer contains a mixture of sodium phosphate salt and potassium phosphate salt.

[0030] As used herein, the term "buffer" refers to a component that maintains the pH of the medium to which it is added at a constant level. In the context of this disclosure, gentisic acid, ascorbic acid, L-methionine and pyridoxine, their salts or aqueous solutions are not considered buffers. In one embodiment, the pH of the formulation is about 4 to about 8. In one embodiment, the pH of the formulation is about 4. In another embodiment, the pH of the formulation is about 4.5. In another embodiment, the pH of the formulation is about 5.0. In one embodiment, the pH of the formulation is about 5.5. In another embodiment, the pH of the formulation is about 5.6. In another embodiment, the pH of the formulation is about 5.7. In another embodiment, the pH of the formulation is about 5.8. In another embodiment, the pH of the formulation is about 5.9. In another embodiment, the pH of the formulation is about 6.0. In another embodiment, the pH of the formulation is about 6.1. In another embodiment, the pH of the formulation is about 6.2. In another embodiment, the pH of the formulation is about 6.3. In another embodiment, the pH of the formulation is about 6.4. In another embodiment, the pH of the formulation is about 6.5. In another embodiment, the pH of the formulation is about 7.0. In another embodiment, the pH of the formulation is about 7.5. In another embodiment, the pH of the formulation is about 8.0. In a preferred embodiment, the pH of the formulation is about 6.0. In another preferred embodiment, the pH of the formulation is about 5.0.

[0031] The inventors have found that when compound of formula (I) is formulated as an aqueous solution, the compound is relatively unstable and prone to oxidation and decomposition.One approach to overcome the observed instability may be to add one or more components to the formulation, which are antioxidants and / or stabilizers, but the inclusion of additional components in the formulation brings about potential reactivity problems between compound of formula (I) and these added components.For example, adding antioxidants may actually react with compound of formula (I), thus potentially changing the structure and function of the compound, which is undesirable. However, the inventors have now found that the addition of certain stabilizers may be sufficient in some cases to provide formulations containing the compound of formula (I).As disclosed herein, formulations of the compound of formula (I) containing stabilizers such as gentisic acid, ascorbic acid, L-methionine or pyridoxine are contemplated, since these stabilizers do not appear to react with the compound of formula (I) and can provide the necessary stability.

[0032] However, it has now been found that a formulation containing a buffer and a compound of formula (I) provides the compound with the necessary stability. Thus, the inventors have found that, in addition to providing a formulation with a suitable pH for parenteral administration, the presence of a buffer provides a formulation in which the compound of formula (I) has the necessary stability. Surprisingly, the inventors have found that, although the addition of stabilizers such as gentisic acid, ascorbic acid and other agents described herein can provide the necessary stability, stability of the formulation can also be achieved by the use of a buffer alone, i.e., in the absence of a stabilizer.

[0033] Method for preparing the complex of formula (I) The present invention also relates to a method for preparing a radiolabeled complex of the compound of formula (I), and a formulation thereof. As mentioned above, the compound of formula (I) can be complexed with a radioisotope, such as Cu ion. Thus, the present invention relates to a method for preparing a formulation comprising a compound of formula (I) complexed with a Cu radioisotope, comprising: i) adding an amount of a compound of formula (I) to an acetate buffer; ii) adding a solution of Cu radioisotope in hydrochloric acid to the compound of formula (I) and acetate buffer; and iii) heating the mixture of step ii) for a time and under conditions to form a complex of formula (I) with the Cu radioisotope. The present invention provides a method comprising:

[0034] [ka] Formula (I) The present invention also provides a method for preparing a formulation comprising a compound of formula (I) complexed with a Cu radioisotope, comprising the steps of: i) adding an amount of a compound of formula (I) to a phosphate buffer solution; ii) adding a solution of Cu radioisotope in hydrochloric acid to the compound of formula (I) and a phosphate buffer solution; and iii) reacting the mixture of step ii) for a time and under conditions to form a complex of formula (I) with the Cu radioisotope. The present invention provides a method comprising:

[0035] [ka] Formula (I) In certain embodiments, the compound of formula (I) has the structure of a compound of formula (Ia). [ka] Formula (Ia)

[0036] In one embodiment, the method further comprises the step of adding a sodium ascorbate solution to the mixture of the compound of formula (I) and the Cu radioisotope once the reaction between the compound of formula (I) and the Cu radioisotope is complete. The compound of formula (I) may be provided as part of a stock solution. Before preparing the stock solution of formula (I), the compound may be subjected to a drying process, such as lyophilization. The compound of formula (I) may be dissolved in a mixture of ethanol and water to produce a stock solution of the compound of formula (I). In one embodiment, the compound of formula (I) is dissolved in a mixture of ethanol and water, where the ethanol and water are present in a ratio of about 1:1. In one embodiment, the compound of formula (I) is provided as a stock solution at a concentration of about 1 nmol / μL.

[0037] The compound of formula (I) may be present in an amount between about 1 nmol and about 10 nmol. In one embodiment, the compound of formula (I) is present in an amount of about 1 nmol. In another embodiment, the compound of formula (I) is present in an amount of about 2 nmol. In another embodiment, the compound of formula (I) is present in an amount of about 3 nmol. In another embodiment, the compound of formula (I) is present in an amount of about 4 nmol. In another embodiment, the compound of formula (I) is present in an amount of about 5 nmol. In another embodiment, the compound of formula (I) is present in an amount of about 6 nmol. In another embodiment, the compound of formula (I) is present in an amount of about 7 nmol. In another embodiment, the compound of formula (I) is present in an amount of about 8 nmol. In another embodiment, the compound of formula (I) is present in an amount of about 9 nmol. In another embodiment, the compound of formula (I) is present in an amount of about 10 nmol. Those skilled in the art will appreciate that the required amount of stock solution of compound formula (I) depends on the initial concentration of the stock solution. Those skilled in the art will also appreciate that larger amounts of the compound of formula (I) may be used and then the amounts of the other reagents, buffers, and solvents may be altered accordingly. In one embodiment, the buffer solution may be an acetate buffer. The acetate buffer used in this method may be prepared from sodium acetate and acetic acid. The acetate buffer maintains the pH in a range suitable for complex formation between the compound of formula (I) and the Cu radioisotope. The pH of the buffer solution may be about 5.0. The acetate buffer may have a concentration of about 1.0 M. The acetate buffer may also contain ethanol. In one embodiment, the acetate buffer contains ethanol in an amount between about 10% and about 30%. In one embodiment, the acetate buffer contains ethanol in an amount of about 10%. In one embodiment, the acetate buffer contains ethanol in an amount of about 20%. In one embodiment, the acetate buffer contains ethanol in an amount of about 30%. In a preferred embodiment, the acetate buffer contains ethanol in an amount of about 20%.

[0038] In another embodiment, the buffer solution may be a phosphate buffer. The phosphate buffer may include a mixture of various phosphate salts or their hydrates. Examples of suitable phosphate salts include sodium dihydrogen phosphate (NaH2PO4), disodium hydrogen phosphate (Na2HPO4), potassium dihydrogen phosphate (KH2PO4) and dipotassium hydrogen phosphate (K2HPO4). In one embodiment, the phosphate buffer contains a sodium phosphate salt. In another embodiment, the phosphate buffer contains a potassium phosphate salt. In another embodiment, the phosphate buffer contains a mixture of a sodium phosphate salt and a potassium phosphate salt. The phosphate buffer may also contain saline and / or water. In one embodiment, the phosphate buffer contains a mixture of a sodium hydrogen phosphate salt and saline. From a stock solution of the compound of formula (I) in a mixture of ethanol and water, an aliquot containing a certain amount of the compound of formula (I) is taken and mixed with a certain amount of acetate buffer. In one embodiment, the compound of formula (I) is added to the acetate buffer, where the acetate buffer contains about 20% ethanol. In one embodiment, the compound of formula (I) is added to the acetate buffer at room temperature.

[0039] As described above, the compound of formula (I) complexes Cu ions. In one embodiment, the Cu ions are radioisotopes of Cu. In one embodiment, the Cu radioisotopes are 60 In another embodiment, the Cu radioisotope is 61 In another embodiment, the Cu radioisotope is 64 In another embodiment, the Cu radioisotope is 67 The Cu radioisotope is provided as a Cu salt. In one embodiment, the Cu salt is 2+ In one embodiment, the Cu salt is provided as a chloride salt. 64 The Cu radioisotope is provided as a hydrochloric acid solution. In one embodiment, the Cu radioisotope is 64Cu, provided as a hydrochloric acid solution, the hydrochloric acid having a concentration of about 0.02 M. In one embodiment, the Cu radioisotope is 64 In another embodiment, the Cu radioisotope is provided as a CuCl solution, with the hydrochloric acid having a concentration of about 0.02 M. One of ordinary skill in the art will appreciate that the Cu salt may be provided in other concentrations of hydrochloric acid. 2+ In one embodiment, the Cu salt is provided as an acetate salt. 64 Cu]Cu(OAc)2 salt.

[0040] The solution of Cu salt provided as hydrochloric acid solution will have a certain starting radioactivity. The starting radioactivity of the solution may vary depending on the specific batch of radioisotope. Those skilled in the art will understand that the final radioactivity of the compound of formula (I) complexed with Cu ion depends on the radioactivity of the Cu salt used to complex the compound of formula (I), which in turn depends on the radioactivity of the solution of Cu salt in hydrochloric acid. The total radiochemical yield of the complex of formula (I) and copper salt can be determined using the amount of radioactivity initially present in the solution of Cu salt. An aliquot of Cu radioisotope in hydrochloric acid solution is added to the compound of formula (I) in acetate buffer. Those skilled in the art will understand that radiochemical purity can be measured by radio-HPLC or similar methods.

[0041] In one embodiment, 64 The solution of Cu radioisotope has a radioactivity of between about 100 and about 5000 MBq. 64 The solution of Cu radioisotope has a radioactivity of about 100 MBq. 64 The solution of Cu radioisotope has a radioactivity of about 250 MBq. 64 The solution of Cu radioisotope has a radioactivity of about 500 MBq. 64 The solution of Cu radioisotope has a radioactivity of about 750 MBq. 64 The solution of Cu radioisotope has a radioactivity of about 1000 MBq.64 The solution of Cu radioisotope has a radioactivity of about 1500 MBq. 64 The solution of Cu radioisotope has a radioactivity of about 2000 MBq. 64 The solution of Cu radioisotope has a radioactivity of about 2500 MBq. 64 The solution of Cu radioisotope has a radioactivity of about 3000 MBq. 64 The solution of Cu radioisotope has a radioactivity of about 4000 MBq. 64 The solution of Cu radioisotope has a radioactivity of about 5000 MBq.

[0042] In one embodiment, 61 The solution of Cu radioisotope has a radioactivity of between about 100 and about 5000 MBq. 61 The solution of Cu radioisotope has a radioactivity of about 100 MBq. 61 The solution of Cu radioisotope has a radioactivity of about 250 MBq. 61 The solution of Cu radioisotope has a radioactivity of about 500 MBq. 61 The solution of Cu radioisotope has a radioactivity of about 750 MBq. 61 The solution of Cu radioisotope has a radioactivity of about 1000 MBq. 61 The solution of Cu radioisotope has a radioactivity of about 1500 MBq. 61 The solution of Cu radioisotope has a radioactivity of about 2000 MBq. 61 The solution of Cu radioisotope has a radioactivity of about 2500 MBq. 61 The solution of Cu radioisotope has a radioactivity of about 3000 MBq. 61 The solution of Cu radioisotope has a radioactivity of about 4000 MBq. 61The solution of Cu radioisotope has a radioactivity of about 5000 MBq.

[0043] In one embodiment, 67 The solution of Cu radioisotope has a radioactivity of between about 100 and about 3000 MBq. 67 The solution of Cu radioisotope has a radioactivity of about 100 MBq. 67 The solution of Cu radioisotope has a radioactivity of about 250 MBq. 67 The solution of Cu radioisotope has a radioactivity of about 500 MBq. 67 The solution of Cu radioisotope has a radioactivity of about 750 MBq. 67 The solution of Cu radioisotope has a radioactivity of about 1000 MBq. 67 The solution of Cu radioisotope has a radioactivity of about 1500 MBq. 67 The solution of Cu radioisotope has a radioactivity of about 2000 MBq. 67 The solution of Cu radioisotope has a radioactivity of about 2500 MBq. 67 The solution of Cu radioisotope has a radioactivity of about 3000 MBq. 67 The solution of Cu radioisotope has a radioactivity of about 4000 MBq. 67 The solution of Cu radioisotope has a radioactivity of about 5000 MBq. The Cu radioisotope may be provided as a hydrochloric acid solution. In one embodiment, the Cu radioisotope is provided in a hydrochloric acid solution having a concentration between about 0.01M and about 0.05M. In one embodiment, the concentration of the hydrochloric acid solution is about 0.01M. In another embodiment, the concentration of the hydrochloric acid solution is about 0.02M. In another embodiment, the concentration of the hydrochloric acid solution is about 0.03M. In another embodiment, the concentration of the hydrochloric acid solution is about 0.04M. In another embodiment, the concentration of the hydrochloric acid solution is about 0.05M. In a further embodiment, the concentration of the hydrochloric acid solution is between about 0.02M and about 0.05M.

[0044] The solution containing the mixture of Cu radioisotope, compound of formula (I) and acetate buffer is then mixed for a certain time at a certain temperature to allow complexation of the compound of formula (I) with the Cu radioisotope. The solution can be mixed using a suitable device. For example, an Eppendorf tube may be a suitable container when small amounts are used, so that an Eppendorf thermomixer can be used to both mix the solution and heat it if necessary. In one embodiment, the solution is mixed at room temperature. In one embodiment, the solution is mixed at about 40° C. The inventors have found that when the solutions are mixed at a temperature of about 40° C., the complexation of the radioisotope is complete within about 5 minutes. A lower temperature of about 21° C., i.e., room temperature, can be used for mixing, and the complexation reaction may not be complete in 5 minutes, but is complete in about 15 minutes. The inventors have also found that temperatures higher than about 40° C., such as 60° C., result in some decomposition of the compound of formula (I) and therefore a lower yield of the complex. In one embodiment, the solution is mixed for about 5 minutes at about 40° C. In one embodiment, the solution is mixed for about 10 minutes at about 40° C. In another embodiment, the solution is mixed for about 15 minutes at about 40° C. In another embodiment, the solution is mixed for about 10 minutes at about 21° C. In another embodiment, the solution is mixed for about 15 minutes at about 21° C.

[0045] In certain embodiments, the compound of formula (I) is added to a phosphate buffer solution to which a solution containing a Cu radioisotope in hydrochloric acid is added. The mixture containing the compound of formula (I), the phosphate buffer, and the Cu radioisotope is reacted for a period of time and under conditions to provide a complex of formula (I) and the Cu radioisotope. In one embodiment, the solution is mixed at room temperature. In one embodiment, the solution is mixed at room temperature for about 10 minutes. In another embodiment, the solution is mixed at room temperature for about 15 minutes. In another embodiment, the solution is mixed at room temperature for about 20 minutes. In a further embodiment, the solution is mixed at room temperature for about 25 minutes. Once the complex of formula (I) and the Cu radioisotope is formed, the solution is diluted with sodium ascorbate solution. The addition of sodium ascorbate introduces a reducing agent into the mixture, which provides a radiostabilising effect on the complex of formula (I) and the Cu radioisotope. This then enhances the stability of the entire formulation and provides a longer shelf life for the complex-containing formulation. The sodium ascorbate solution may have a concentration between about 25 mg / mL and about 75 mg / mL. In one embodiment, the sodium ascorbate solution may have a concentration of about 25 mg / mL. In another embodiment, the sodium ascorbate solution may have a concentration of about 50 mg / mL. In another embodiment, the sodium ascorbate solution may have a concentration of about 75 mg / mL. A certain amount of sodium ascorbate solution of a particular concentration is added to ensure that the remaining Cu radioisotope is sufficiently diluted. One of ordinary skill in the art will appreciate that the amount of solution added will depend on the amount of uncomplexed Cu radioisotope and the concentration of the sodium ascorbate solution.

[0046] The inventors have found that the method of preparing a compound of formula (I) complexed with a Cu radioisotope disclosed herein allows for efficient radiolabeling of the compound and allows for obtaining a high radiochemical yield. The inventors have found that the complex formation between the compound of formula (I) and the Cu radioisotope is faster when an acetate buffer containing a certain amount of ethanol is used. According to one embodiment of the present invention, the method comprises adding the compound of formula (I) to an acetate buffer containing ethanol. In one embodiment, the method comprises: i) adding an amount of a compound of formula (I) to an acetate buffer containing ethanol; ii) In hydrochloric acid [ 64 adding a solution of Cu]CuCl2 to a compound of formula (I) and an acetate buffer containing ethanol; and iii) heating the mixture of step ii) at 40° C. for about 5 minutes. Includes.

[0047] In another embodiment, the method comprises: i) adding an amount of a compound of formula (I) to a phosphate buffer containing ethanol and sodium gentisate; ii) In hydrochloric acid [ 64 adding a solution of Cu]CuCl2 to a compound of formula (I) and a phosphate buffer containing sodium gentisate; and iii) reacting the mixture of step ii) for a time and under conditions to form a complex of formula (I) with the Cu radioisotope. Includes. Process for purifying the complex of formula (I) Upon completion of the process of preparing the compound of formula (I) complexed with a Cu radioisotope, the complex must be purified and isolated. Those skilled in the art will appreciate that during the purification and isolation process, losses of material may occur, thus reducing the overall chemical and radiochemical yield. These losses may result from transport steps, loss of material in syringes and other equipment used in the purification process, or retention of material in the reaction vessel. The purification process typically involves a filtration step using a solid phase medium, and retention of material by the solid phase often results in a loss of yield. The purification process often relies on washing the solid phase with various solvents to elute the complex, but the use of large amounts of solvent results in a dilute solution of the complex, which is undesirable. Decomposition of the complex may also occur during purification, resulting in a reduced yield of the complex and loss of free radioisotope. The inventors have found that purification of the complex of formula (I) with a Cu radioisotope can be advantageously achieved, resulting in isolation of the complex in high chemical and radiochemical yields.

[0048] According to another aspect of the present invention, there is provided a method for purifying a compound of formula (I) complexed with a Cu radioisotope, comprising the steps of: i) loading a solution of compound of formula (I) complexed with a Cu radioisotope into a solid phase extraction cartridge; ii) eluting the compound of formula (I) complexed with the Cu radioisotope using an eluent containing water, ethanol and sodium chloride. A method is provided, comprising: [ka] Formula (I)

[0049] In one embodiment of the method, the compound of formula (I) has the structure of a compound of formula (Ia): [ka] Formula (Ia)

[0050] In one embodiment, a solution of the compound of formula (I) complexed with a Cu radioisotope is obtained according to another embodiment of the present invention. Upon completion of the reaction to prepare the complex of formula (I) with a Cu radioisotope, the obtained solution is subjected to a purification process. The solution containing the complex of formula (I) and Cu radioisotope is loaded into a solid-phase extraction cartridge. The solid-phase extraction cartridge contains a stationary phase that retains the complex and other components present in the solution. As used herein, the term "stationary phase" refers to a resin-like material that is retained in the solid-phase extraction cartridge and allows separation of compounds based on their polarity. The solid phase extraction process described herein can use a reversed phase stationary phase. As used herein, the term "reverse phase" with respect to a stationary phase refers to a stationary phase that is hydrophobic in nature, such that the stationary phase has an affinity for hydrophobic or uncharged molecules. Examples of reversed phase stationary phases can include Waters Sep-Pak cartridges, such as C8, C18, light C18, light CN, light tC2, or HLB cartridges. Prior to loading the solution containing the complex of formula (I), the cartridge is prepared by washing with ethanol, drying with air, and equilibrating with water. In one embodiment, the solid phase extraction cartridge is a Waters C18 cartridge. In another embodiment, the solid phase extraction cartridge is a Waters tC2 cartridge. In another embodiment, the solid phase extraction cartridge is a Waters CN cartridge. In another embodiment, the solid phase extraction cartridge is a Waters HLB cartridge.

[0051] The purified solution containing the complex of formula (I) and Cu radioisotope can then be used to produce a formulation containing the complex. For example, one or more pharma- ceutically acceptable diluents, adjuvants and / or excipients may be added to the solution containing the complex of formula (I) and Cu radioisotope. The diluents, adjuvants and excipients must be "acceptable" in the sense of being compatible with the other components of the composition and not harmful to the recipient. Pharmaceutical carriers for preparing pharmaceutical compositions are known in the art, as described in textbooks such as Remington's Pharmaceutical Sciences, 20th Edition, Williams & Wilkins, Pennsylvania, USA. The carrier depends on the route of administration, and those skilled in the art can easily determine the most suitable formulation in each particular case.

[0052] The reference in this specification to any prior document (or information derived from a prior document) or to any public knowledge is not, and should not be construed as, an acknowledgement or admission, or any form of suggestion that the prior document (or information derived from a prior document) or public knowledge forms part of the general knowledge within the scope of the present specification. Throughout this specification and the appended claims, unless the context requires otherwise, the word "comprise" and variations such as "comprises" and "comprising" will be understood to imply the inclusion of a stated integer or step or group of integers or steps, but not the exclusion of any other integer or step or group of integers or steps. EXAMPLES

[0053] Overall experiment details Sodium acetate buffer for radiolabeling was prepared using sodium acetate (TraceSELECT, Fluka, batch number BCBM4793V), acetic acid (TraceSELECT, Fluka, batch number BCBM5177V) and MilliQ water in acid-washed glass bottles and stored in acid-washed plastic bottles. All buffers were stored at 2–4 °C between uses. Phosphate buffer for radiolabeling was prepared using disodium hydrogen phosphate (anhydrous), sodium dihydrogen phosphate, and TraceSELECT water. All buffers were stored at room temperature between uses. Copper-64 ( 64 Cu) was obtained from SAHMRI, SA, Australia, in 0.02 M HCl with a starting activity of 2.39 GBq@08:39 in a volume of 450 μL. 64 Cu]CuCl2, batch number 19-0075-902R. SPE purification of the radiolabeled product was performed using a Waters Sep-Pak cartridge light C8 (Lot No.: 002836047A). The cartridge was conditioned by washing with EtOH (10 mL) followed by equilibration with a bolus of Air (3×10 mL), then MilliQ HO (10 mL) and Air (3×10 mL).

[0054] MeCN for HPLC (Honeywell, Lot No.: S1RA1H), and trifluoroacetic acid for HPLC (TFA, ReagentPlus, 99%, Sigma Aldrich, Lot No.: SHBG2783V), (+)-L-sodium ascorbate (Sigma Aldrich, >99%, Lot No.: BCBV4424), L-methionine (Sigma Aldrich, >99.5%, Lot No.: BCBS2107V) and gentisic acid sodium salt hydrate (Sigma Aldrich, >99%, Lot No.: MKCC2280) were used as received. All HPLC mobile phases were prepared before use, filtered (using 0.45 μm aqueous or organic filters) and degassed by a combination of sonication under vacuum for 10 min. All EtOH used was 100% ethyl alcohol (molecular biology grade). All syringes used were "B Braun Injekt-F". The elution buffer was prepared as 1:1 EtOH:H2O + 0.9% NaCl. All reaction vials were acid washed prior to use. Plastic microcentrifuge tubes were filled with 4M HCl and left to stand at least overnight, the 4M HCl was removed, and the vials were thoroughly washed with MilliQ H2O and dried in an oven at 50 °C. After drying, the vials were sealed to prevent further contamination. Glassware was acid washed by immersion in 4M HNO3 for a minimum of 12 hours, the 4M HNO3 was decanted into an appropriate waste container, and then the glassware was thoroughly washed with MilliQ H2O and dried in an oven at 50 °C. After drying, the glassware was sealed to prevent further contamination. A stock solution of Sar-bis(PSMA), i.e., compound of formula (Ia), in EtOH:H2O (1:1) was prepared to obtain a solution containing compound of formula (Ia) at a concentration of 1 nmol / μL.

[0055] Radiolabeling of formula (I) with Cu radioisotope Example 1 To an acid-washed 500 μL microcentrifuge tube, labeling buffer (acetate buffer, 50 μL, 1 M, pH 5.0) was added, followed by 10 μL of the stock solution of Formula I. The buffer solution contained [ 64 Cu]CuCl2 (25 μL, 116 MBq) was added. The microcentrifuge tube was sealed and the radioactivity present in the reaction was measured using a dose calibrator. The tube was transferred to an Eppendorf Thermomixer C and heated at 40 °C for 20 min. At 20 min the reaction was removed from the Thermomixer and a sample (5 μL) was taken from the reaction, diluted with 1:1 EtOH:H2O (5 μL) and injected into a radio-HPLC system (QC1, 5 μL). The reaction mixture was at room temperature while a final analysis was performed at 7 min to determine if the radiochemical yield was greater than 95%.

[0056] Example 2 To an acid-washed 500 μL microcentrifuge tube, labeling buffer (acetate buffer, 50 μL, 1 M, pH 5.0) was added, followed by 5 μL of the stock solution of Formula I. The buffer solution contained [ 64 Cu]CuCl2 (25 μL, 109 MBq) was added. The microcentrifuge tube was sealed and the radioactivity present in the reaction was measured using a dose calibrator. The tube was transferred to an Eppendorf Thermomixer C and heated at 40 °C for 20 min. At 20 min the reaction was removed from the Thermomixer and a sample (5 μL) was taken from the reaction, diluted with 1:1 EtOH:H2O (5 μL) and injected into a radio-HPLC system (QC1, 5 μL). The reaction mixture was at room temperature while a final analysis was performed at 7 min to determine if the radiochemical yield was greater than 95%. Example 3 To an acid-washed 1500 μL microcentrifuge tube, labeling buffer (acetate buffer, 600 μL, 1 M, pH 5.0) was added, followed by 20 μL of the stock solution of Formula I. The buffer solution contained [ 64Cu]CuCl2 (300 μL, 1136 MBq) was added. The microcentrifuge tube was sealed and the radioactivity present in the reaction was measured using a dose calibrator. The tube was transferred to an Eppendorf Thermomixer C and heated at 40 °C for 20 min. At 20 min the reaction was removed from the Thermomixer and a sample (5 μL) was taken from the reaction, diluted with 1:1 EtOH:H2O (5 μL) and injected into a radio-HPLC system (QC1, 5 μL). The reaction mixture was at room temperature while a final analysis was performed at 7 min to determine if the radiochemical yield was greater than 95%.

[0057] Example 4 To an acid-washed 500 μL microcentrifuge tube, labeling buffer (20% EtOH in acetate buffer, 100 μL, 1 M, pH 5.0) was added, followed by 10 μL of the stock solution of Formula I. The buffer solution contained [ 64 Cu]CuCl2 (50 μL, 183 MBq) was added. The microcentrifuge tube was sealed and the radioactivity present in the reaction was measured using a dose calibrator. The tube was transferred to an Eppendorf Thermomixer C and heated at 40 °C for 20 min. At 20 min the reaction was removed from the Thermomixer and a sample (5 μL) was taken from the reaction, diluted with 1:1 EtOH:H2O (5 μL) and injected into a radio-HPLC system (QC1, 5 μL). The reaction mixture was at room temperature while a final analysis was performed at 7 min to determine if the radiochemical yield was greater than 95%. Example 5 To an acid-washed 500 μL microcentrifuge tube, labeling buffer (acetate buffer, 100 μL, 1 M, pH 5.0) was added, followed by 5 μL of the stock solution of Formula I. The buffer solution contained [ 64Cu]CuCl2 (50 μL, 174 MBq) was added. The microcentrifuge tube was sealed and the radioactivity present in the reaction was measured using a dose calibrator. The tube was transferred to an Eppendorf Thermomixer C and heated at 21 °C for 20 min. At 5 and 15 min, aliquots (5 μL) were taken for analysis to determine if the reaction was complete. These samples were diluted with 1:1 EtOH:H2O (5 μL) and injected into the radio-HPLC system (QC1, 5 μL). The reaction mixture was again placed in the thermomixer while a final analysis was performed at 7 min to determine if the radiochemical yield was greater than 95%.

[0058] Example 6 A solution of Sar-bis(PSMA) (50 μg, 24.8 nmol) in 0.1 M Na / Na phosphate buffer (5 mL) containing sodium gentisate (5 mg, 0.03 mmol) was diluted with [ 64 Cu]CuCl2 (NMT 500 μL, NMT 5000 MBq) was added at room temperature. The resulting mixture was allowed to react at room temperature for up to 25 minutes. Upon completion, the reaction mixture was quenched with 50 mg / mL sodium ascorbate solution (15 mL). The resulting mixture was then transferred to a sterile vial through a 0.22 μm vent filter and 64 A compound of formula (I) complexed with a Cu radioisotope is obtained.

[0059] Example 7 A solution of Sar-bis(PSMA) (50 μg, 24.8 nmol) in 0.1 M Na / Na phosphate buffer (4.5 mL) containing sodium gentisate (5 mg, 0.03 mmol) and ethanol (neat, 0.5 mL) was diluted with [ 64 Cu]CuCl2 (NMT 500 μL, NMT 5000 MBq) was added at room temperature. The resulting mixture was allowed to react at room temperature for up to 25 minutes. Upon completion, the reaction mixture was quenched with 50 mg / mL sodium ascorbate solution (15 mL). The resulting mixture was then transferred to a sterile vial through a 0.22 μm vent filter and 64A compound of formula (I) complexed with a Cu radioisotope is obtained. Purification procedure Example 8 The solution obtained in Example 1 was purified using a C8 SPE cartridge and the product was eluted with 0.5 mL of 1:1 EtOH:H2O + 0.9% NaCl. 62% of the product was eluted from the SPE and shown to have a high radiochemical purity of 94.3% with zero "free copper". 4% was lost in the dilution / fill syringe, 12% remained in the reaction vial, and 9% was lost to the SPE. Less than 1% was lost in the SPE fill and wash steps.

[0060] Example 9 The solution obtained in Example 2 was purified using a C8 SPE cartridge and the product was eluted with 0.5 mL of 1:1 EtOH:H2O + 0.9% NaCl. 73% of the product was eluted from the SPE, showing a high radiochemical purity of 94.3% with 0.2% "free copper". 7% was lost in the dilution / fill syringe, 1% remained in the reaction vial, and 14% was lost to the SPE. Less than 1% was lost in the SPE fill and wash steps. Example 10 The solution obtained in Example 3 was purified using a C8 SPE cartridge and the product was eluted with 0.5 mL of 1:1 EtOH:H2O + 0.9% NaCl. 64% of the product was eluted from the SPE and shown to have a high radiochemical purity of 96.6% with 0.1% "free copper". 4% was lost in the dilution / filling syringe and 1.5% did not leave the reaction vial.

[0061] Example 11 The solution obtained in Example 4 was purified using a C8 SPE cartridge and the product was eluted with 0.5 mL of 1:1 EtOH:H2O + 0.9% NaCl. 71% of the product was eluted from the SPE and shown to have zero "free copper" and a high radiochemical purity of 96.3%. 6% was lost in the dilution / fill syringe, 3% remained in the reaction vial, and 15% was lost to the SPE. Less than 1% was lost in the SPE fill and wash steps. Example 12 The solution obtained in Example 5 was purified using a C8 SPE cartridge and the product was eluted with 0.5 mL of 1:1 EtOH:H2O + 0.9% NaCl. 59% of the product was eluted from the SPE, showing a high radiochemical purity of 96.9% with 0.1% "free copper". 11% was lost in the dilution / fill syringe, 7% remained in the reaction vial, and 20% was lost to the SPE. Less than 1% was lost in the SPE fill and wash steps.

[0062] Preparation of formulations Example 13 Aliquots of the purified solution of Example 10 were taken and diluted with a mixture of ethanol in saline to give a final concentration of approximately 10% ethanol in saline. One of gentisic acid (0.63 mg / mL), ascorbic acid (10 mg / mL), or L-methionine (3 mg / mL) was added and the radiochemical purity of each sample was monitored over a 48 hour period.

Claims

1. 1. An aqueous formulation for parenteral administration comprising a compound of formula (I) or a salt thereof complexed with Cu ions, the formulation further comprising at least one of gentisic acid, ascorbic acid, L-methionine, pyridoxine, or salts thereof. 【Chemistry 1】 Formula (I)

2. 1. An aqueous formulation for parenteral administration comprising a compound of formula (I) or a salt thereof complexed with Cu ions, said formulation further comprising a buffer solution. 【Chemistry 2】 Formula (I)

3. 2. The formulation of claim 1, wherein the compound of formula (I) has the structure of formula (Ia): 【Chemistry 3】 Formula (Ia)

4. The aqueous preparation according to any one of claims 1 to 3, comprising gentisic acid or a salt thereof.

5. The aqueous preparation according to any one of claims 1 to 3, which comprises ascorbic acid or a salt thereof.

6. The aqueous preparation according to any one of claims 1 to 3, comprising L-methionine or a salt thereof.

7. The aqueous preparation according to any one of claims 1 to 6, wherein the Cu ions are a Cu radioisotope.

8. Cu radioisotope 64 The aqueous formulation of claim 7, wherein the compound is Cu.

9. Cu radioisotope 67 The aqueous formulation of claim 7, wherein the compound is Cu.

10. 10. The formulation of any one of claims 1 to 9, having a pH between about 4 and about 8.

11. A method for preparing a formulation comprising a compound of formula (I) complexed with a Cu radioisotope, comprising the steps of: i) adding an amount of a compound of formula (I) to an acetate buffer solution; ii) adding a solution of Cu radioisotope in hydrochloric acid to the compound of formula (I) and acetate buffer; and iii) heating the mixture of step ii) for a time and under conditions to form a complex of formula (I) with the Cu radioisotope. The method comprising: 【Chemistry 4】 Formula (I)

12. A method for preparing a formulation comprising a compound of formula (I) complexed with a Cu radioisotope, comprising the steps of: i) adding an amount of a compound of formula (I) to a phosphate buffer solution; ii) adding a solution of Cu radioisotope in hydrochloric acid to the compound of formula (I) and a phosphate buffer solution; and iii) reacting the mixture of step ii) for a time and under conditions to form a complex of formula (I) with the Cu radioisotope. The method comprising: 【Chemistry 5】 Formula (I)

13. iv) adding a sodium ascorbate solution to the mixture of the compound of formula (I) and the Cu radioisotope.

13. The method of claim 11 or 12, further comprising:

14. The method of any one of claims 11 to 13, wherein the compound of formula (I) has the structure of formula (Ia): 【Chemistry 6】 Formula (Ia)

15. Cu radioisotope 64 The method according to any one of claims 11 to 14, wherein the metal is Cu.

16. Cu radioisotope 61 The method according to any one of claims 11 to 14, wherein the metal is Cu.

17. Cu radioisotope 67 The method according to any one of claims 11 to 14, wherein the metal is Cu.

18. Cu radioisotope is [ 64 Cu]CuCl 2 The method of any one of claims 11 to 14, wherein the compound is

19. The method of any one of claims 11 to 18, wherein the pH of the formulation is maintained in the range between about 4 and about 8.

20. A method for purifying a compound of formula (I) complexed with a Cu radioisotope, comprising the steps of: i) loading a solution of compound of formula (I) complexed with a Cu radioisotope into a solid phase extraction cartridge; ii) eluting the compound of formula (I) complexed with the Cu radioisotope using an eluent containing water, ethanol and sodium chloride. The method comprising: 【Chemistry 7】 Formula (I)

21. 21. The method of claim 20, wherein the compound of formula (I) has the structure of formula (Ia): 【Chemistry 8】 Formula (Ia)

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