Composition for scalp and / or hair

Abstract

To provide a composition for promoting hair growth.SOLUTION: Provided is a composition for scalp and / or hair, comprising one or more selected from Ribes nigrum, Aspalathus linearis, and Swertia japonica (Schult.) Makino.SELECTED DRAWING: Figure 1

Description

[Technical field]

[0001] The present invention relates to a composition to be applied to the scalp and / or hair. [Background technology]

[0002] New hair that grows from the hair root grows for a certain period of time, then stops growing and falls out, repeating this cycle. To keep hair healthy, it is important to maintain a normal hair cycle.

[0003] It is well known that aging causes the above hair cycle to not function normally, and hair thinning and hair loss progresses, but in recent years, due to the increase in social stress and the disturbance of lifestyle habits, even relatively young people have more and more cases of hair cycle not functioning normally, and hair thinning and hair loss progresses.In addition, in recent years, it has been revealed that not only aging but also obesity promotes hair thinning and hair loss (see Non-Patent Document 1).

[0004] Regarding thinning hair and hair loss, various methods for preventing and treating the condition have been proposed, and the development of effective hair growth agents and the like is underway (for example, Patent Document 1).

[0005] In addition, it is known that various moisturizing agents and oils are blended in hair growth agents to maintain the moisturizing feeling of the scalp and hair. However, there is a concern that some ingredients for maintaining the moisturizing feeling may make the scalp and hair sticky and cause a poor feeling of use. Therefore, development of hair growth agents that maintain a moisturizing feeling and have an excellent feeling of use is being promoted. For example, it has been disclosed that a hair growth agent blended with an extract of loquat leaves exhibits a hair growth effect and is not sticky when used (Patent Document 2). However, this technology is insufficient to exhibit an excellent moisturizing feeling and feeling of use, and further development of hair growth agents is required. [Prior art documents] [Patent documents]

[0006] [Non-Patent Document 1] http: / / www.qlifepro.com / news / 20210625 / obesity-accelerates-hair-thinning.html [Patent Document 1] Patent Publication No. 2021-172596 [Patent Document 2] JP 2010-105932 A Summary of the Invention [Problem to be solved by the invention]

[0007] An object of the present invention is to provide a novel composition that promotes hair growth. [Means for solving the problem]

[0008] The present inventors discovered that black currant, rooibos and Swertia japonica have an effect of promoting the expression of COL17A1 (collagen type XVII alpha 1 chain) and found that this effect promotes hair growth, thereby completing the present invention.

[0009] That is, the present invention is as follows. [1] A composition for the scalp and / or hair, comprising one or more selected from black currant, rooibos and Swertia japonica. [2] The composition described in [1], which is a hair growth composition. [3] The composition described in [1] or [2], characterized in that it is a composition for promoting COL17A1 expression. [4] The composition described in any one of [1] to [3], which has a moisturizing effect. [5] The composition described in any one of [1] to [4], which is an external preparation. Effect of the Invention

[0010] The composition of the present invention promotes hair growth through the promoting effect of COL17A1 expression. The composition of the present invention promotes hair growth through the promoting effect of COL17A1 expression, and furthermore, can exert an excellent moisturizing feeling. [Brief description of the drawings]

[0011] [Figure 1] FIG. 1 shows the expression level (relative value) of COL17 gene in the composition of the present invention (black currant). [Diagram 2] FIG. 1 shows the expression level (relative value) of the COL17 gene in the composition of the present invention (rooibos). [Diagram 3] FIG. 1 shows the expression level (relative value) of the COL17 gene in the composition of the present invention (Switzerland). [Figure 4] FIG. 2 is a graph showing the rate of change in stratum corneum moisture content for the compositions of the present invention (black currant, rooibos). DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0012] The composition of the present invention is applied to the scalp and / or hair, and is characterized by containing one or more selected from black currant, rooibos, and Swertia japonica (hereinafter, sometimes referred to as the components of the present invention). It is preferable that the composition of the present invention contains two or more components of the present invention in order to enjoy the effects of the composition more effectively.

[0013] The composition of the present invention promotes hair growth through the promoting effect of COL17A1 expression.

[0014] Hair follicles play an important role in hair growth, and these hair follicles are maintained by the proliferation of hair follicle stem cells, which are the source of hair, and their differentiation into hair matrix cells. COL17A1 acts on the normal cell division (proliferation and differentiation into hair matrix cells) of hair follicle stem cells. Therefore, the composition of the present invention can promote the expression of COL17A1, and thereby promotes normal cell division of hair follicle stem cells and inhibits aging of hair follicle stem cells, thereby exerting a hair growth effect.

[0015] In addition, when COL17A1 is decreased, the division of hair follicle stem cells is altered, and the hair follicle stem cells differentiate into epidermal cells, resulting in a decrease in the number of hair follicle stem cells. In aged hair follicle stem cells, this COL17A1 is decreased. Therefore, the composition of the present invention can be said to exhibit a hair growth effect by promoting the expression of COL17A1, inhibiting the decrease in the number of hair follicle stem cells, and inhibiting aging.

[0016] Therefore, the composition of the present invention can be used as a composition for hair growth, a composition for promoting COL17A1 expression, etc. The concept of hair growth in the present invention includes hair growth and hair care.

[0017] [Cassis] Black currant (scientific name: Ribes nigrum) is a plant classified in the Ribes genus of the family Ribesaceae, and is also called black currant or black currant. In the present invention, various parts of the plant, such as the fruit, seeds, leaves, and stems, can be used, but it is particularly preferable to use the fruit, since it can more effectively promote the expression of COL17A1, and can also exhibit excellent moisturizing effects.

[0018] Blackcurrant can be used, for example, as a crushed product, a squeezed juice, an extract, etc. Examples of crushed products include a dried powder and a finely cut product. The squeezed juice and extract may be in a liquid form, but can also be used in a paste form or a dried powder (juice powder, extract powder). In the present invention, an extract is preferred because it can better enjoy the effects of the present invention and can be easily blended into a composition. The extract can be obtained by extraction using a suitable solvent, and the solvent is not particularly limited. However, hydrophilic solvents such as water, ethanol, aqueous ethanol, 1,3-butylene glycol (BG), and aqueous BG are preferred because it can better enjoy the effects of the present invention and can be easily blended into a composition. The temperature of each solvent can be appropriately set at room temperature to below the boiling point.

[0019] [Rooibos] Rooibos (scientific name: Aspalathus linearis) is a plant belonging to the genus Aspalathus of the family Fabaceae, and has conifer-like leaves. In the present invention, various parts of the plant can be used, but it is preferable to use the whole plant, since it can more effectively promote the expression of COL17A1, and also has excellent moisturizing properties.

[0020] Rooibos can be used, for example, as a crushed product, a squeezed juice, an extract, etc. Examples of crushed products include a dried powder and a finely cut product. The squeezed juice and extract may be in a liquid form, but can also be used in a paste form or a dried powder (juice powder, extract powder). In the present invention, an extract is preferred because it can better enjoy the effects of the present invention and can be easily blended into a composition. The extract can be obtained by extraction using a suitable solvent, and the solvent is not particularly limited. However, hydrophilic solvents such as water, ethanol, aqueous ethanol, 1,3-butylene glycol (BG), and aqueous BG are preferred because it can better enjoy the effects of the present invention and can be easily blended into a composition. The temperature of each solvent can be appropriately set at room temperature to below the boiling point.

[0021] [Assembly] Swertia japonica (Schult.) Makino is a biennial plant of the genus Swertia in the family Gentianaceae, distributed in China, the Korean peninsula, and Japan, and is used as a medicinal herb. In the present invention, various parts can be used, but it is preferable to use the whole plant, as this can more effectively promote the expression of COL17A1. The extract (extracted extract) obtained from the whole plant of Swertia japonica during the flowering period is also called swerthianin.

[0022] Swertia japonica can be used, for example, as a pulverized product, a squeezed juice, an extract, etc. Examples of pulverized products include a dried powder and a finely cut product. The squeezed juice and extract may be in a liquid form, but can also be used in a paste form or a dried powder form (juice powder, extract powder). In the present invention, an extract is preferred because it can better enjoy the effects of the present invention and can be easily blended into a composition. The extract can be obtained by extraction using a suitable solvent, and the solvent is not particularly limited. However, hydrophilic solvents such as water, ethanol, aqueous ethanol, 1,3-butylene glycol (BG), and aqueous BG are preferred because it can better enjoy the effects of the present invention and can be easily blended into a composition. The temperature of each solvent can be appropriately set within the range of room temperature to the boiling point or lower.

[0023] The composition of the present invention can be produced by a known method by adding other components, if necessary, in addition to the components of the present invention. Examples of other components include water, astringents, bactericides, antibacterial agents, ultraviolet absorbers, moisturizers, cell activators, anti-inflammatory agents, antioxidants, active oxygen removers, oils and fats, waxes, hydrocarbons, fatty acids, alcohols, esters, surfactants, fragrances, and plant extracts other than black currant, rooibos, and Swertia japonica.

[0024] The composition of the present invention can be used as an external preparation such as a medicine (including quasi-drugs) or a cosmetic. The external preparation is not particularly limited as long as it can be applied to the scalp and / or hair, and examples of the form include liquid, ointment, cream, gel, etc. Specific examples include shampoo, conditioner, rinse, treatment (including leave-in type), hair lotion, hair emulsion (hair cream), hair gel, hair cream, hair oil, hair pack, hair mask, hair tonic, hair liquid, hair spray, hair styling product, hair growth product, hair growth product, hair care product, etc.

[0025] The composition of the present invention can be used as a hair growth composition, and the hair growth composition is not particularly limited as long as it contains at least one of the components of the present invention and can be distinguished from other products in terms of being used for hair growth, and for example, the scope of the present invention includes products that display functions related to hair growth on the main body, packaging, instructions, or promotional materials (advertising media) of the product of the present invention. Specifically, examples of such products include, in addition to displays such as hair growth products (hair growth agents), hair growth products (hair growth agents), and hair care products (hair care agents), displays that include phrases such as "give hair firmness and strength," "for those who want firmness and strength," "improve scalp care effects," "care for scalp and hair," "prevent hair loss," "for those who are concerned about thinning hair," and "for healthy hair."

[0026] The composition of the present invention can be used as a composition for promoting COL17A1 expression, and the composition for promoting COL17A1 expression is not particularly limited as long as it contains at least one of the components of the present invention and can be distinguished from other products as a product in that it is used to promote COL17A1 expression, and for example, the scope of the present invention includes a product that displays a function related to promoting COL17A1 expression on the main body, packaging, instructions, or promotional material (advertising medium) of the product of the present invention. Specifically, examples of the display include phrases such as "promotes COL17A1 expression" and "prevents aging of hair follicle stem cells."

[0027] The hair growth composition of the present invention may be one in which the ingredient of the present invention is indicated as an active ingredient, but is not limited to one in which the ingredient of the present invention is indicated as an active ingredient. For example, it may be one in which the active ingredient is not indicated. In addition, those manufactured and sold with the function and use suggested are included in the scope of the present invention. For example, there is a product sold with the experience testimonials of the person who took it, which mention the function and use as personal impressions, posted on the homepage, etc.

[0028] The content of the component of the present invention in the composition of the present invention (total amount when two or more kinds are used in combination) may be appropriately determined within the range in which the effect is exhibited. For example, the component of the present invention may be contained in the composition of the present invention in an amount of 0.0001 to 50 mass%, preferably 0.001 to 20 mass%, more preferably 0.01 to 10 mass%, and even more preferably 0.1 to 5 mass%, calculated as a dry mass. EXAMPLES

[0029] The present invention will be described in more detail below with reference to examples, but the present invention is not limited to these examples.

[0030] [Example 1] In order to confirm the hair growth effect of the composition of the present invention, the COL17A1 expression promoting effect of the components of the present invention was confirmed.

[0031] <Test substance> As the blackcurrant, blackcurrant fruit extract was used, which was obtained by extracting blackcurrant fruit with hydrous BG. As the rooibos, rooibos extract was used, which was extracted from the whole plant of Aspalathus linearis using hydrated BG. As the Swertia japonica, Swertia japonica extract obtained by extracting the whole plant of Swertia japonica with aqueous ethanol was used.

[0032] <Test Method> The test was conducted according to the following steps (1) to (9). (1) In a 37℃, 5% CO2 incubator, 75 cm 2 A human epidermal keratinocyte cell line was cultured in a flask using 10% FBS-DMEM medium.

[0033] (2) The cells were suspended by trypsinization and then transferred to a 75 cm 2 From the flask, transfer 8 x 10 cells to a collagen-coated 96-well plate. 3 100 μL of cells were seeded to give a cell density of 10 cells / well.

[0034] (3) The cells were pre-cultured overnight in a 37°C, 5% CO2 incubator, and after removing the culture supernatant, the cells were washed once with 150 μL of DMEM. DMEM was then added and the cells were cultured for 2 hours.

[0035] (4) After removing the medium from each well, 100 μL / well of medium containing the test substance was added.

[0036] (5) The medium containing the test substance was prepared as follows. The test substance was dissolved in 10% FBS-DMEM to a concentration of 10% (v / v) to prepare a stock solution. The prepared stock solution was further diluted with 10% FBS-DMEM to adjust the concentration to 1%, and after filter sterilization, further dilution was repeated. Media containing 1%, 0.2%, and 0.04% of the test substance were used as samples for addition. In addition, only 10% FBS-DMEM without the test substance was added as a control.

[0037] (6) After adding the medium containing the test substance, the cells were cultured in a 37°C, 5% CO2 incubator for 24 hours, and then the culture supernatant was removed. Each well was washed once with 150 μL of PBS and incubated with RNeasy (R) RNA was extracted using a Mini Kit (QIAGEN).

[0038] (7) From the extracted RNA, ReverTra Ace (R) cDNA was synthesized using qPCR RT Master Mix with gDNA Remover (Toyobo).

[0039] (8) QuantiNova (R) SYBR (R) The expression level of the COL17 gene was evaluated by real-time PCR using the Green PCR Kit (QIAGEN). The internal standard was RRN18S, and primers from QIAGEN were used.

[0040] (9) The relative gene expression level was calculated by setting the control gene expression level as 1.

[0041] The results are shown in Figures 1 to 3. 1 to 3, black currant, rooibos and Swertia japonica all showed a significant promoting effect on COL17A1 expression. Therefore, the composition of the present invention has a promoting effect on COL17A1 expression and promotes hair growth.

[0042] [Example 2] In order to confirm the moisturizing effect of the composition of the present invention, the effect of the components of the present invention in increasing the moisture content of the stratum corneum was confirmed.

[0043] <Test substance> The black currant and rooibos used were the same as those used in Example 1. In addition, as the carrot extract, a ginseng root extract obtained by extracting ginseng root with aqueous ethanol was used.

[0044] <Test Method> (1) The insides of the forearms of three subjects were washed before application of the test substance.

[0045] (2) After leaving it to stand for 15 minutes, the moisture content of the stratum corneum at the planned application sites of the test substance on the inside of the forearms of the three subjects (each 2 cm long and 2 cm wide) was measured using the measuring device described below.

[0046] (3) 0.02 mL of the test substance was applied to each of the sites on the inside of the forearms of the three subjects, and after leaving the site for 15 minutes, the moisture content of the stratum corneum at the sites where the test substance was applied was similarly measured.

[0047] (4) The average value of the test substance application site after use of the skin topical agent was divided by the average value before use, and the resulting value was taken as the rate of change. The rate of change in stratum corneum moisture content is shown in Figure 4. In these figures, the higher the rate of change, the higher the evaluation.

[0048] The stratum corneum moisture content was measured using a Corneometer CM825 (Courage+Khazaka). This stratum corneum moisture content measurement device evaluates the moisture content of the stratum corneum as skin conductance (electrical conductivity, unit: μS). Measurements were performed using the standard method described in the instruction manual that came with the device.

[0049] As shown in Figure 4, both black currant and rooibos increased the moisture content of the stratum corneum. Therefore, the composition of the present invention can increase the moisture content of the stratum corneum and exert a moisturizing effect.

[0050] [Composition Examples 1-1 to 1-5] (Manufacturing of hair growth products) A hair growth agent was produced comprising the components shown in Table 1 below. This hair growth agent exhibits the effects of the present invention.

[0051] [Table 1]

[0052] [Composition Examples 2-1 to 2-5] (Shampoo manufacturing) A shampoo was prepared from the components shown in Table 2 below. This shampoo exhibits the effects of the present invention.

[0053] [Table 2]

[0054] [Composition Examples 3-1 to 3-5] (Treatment manufacturing) A treatment was produced from each component listed in Table 3 below. This treatment exhibits the effects of the present invention.

[0055] [Table 3]

[0056] [Composition Examples 4-1 to 4-5] (Hair lotion manufacturing) A lotion was produced from each component shown in Table 4 below. This hair lotion exhibits the effects of the present invention.

[0057] [Table 4]

[0058] [Composition Examples 5-1 to 5-5] (Manufacture of hair lotion (hair milk)) A milky lotion was produced from each of the components shown in Table 5 below. This hair milky lotion exhibits the effects of the present invention.

[0059] [Table 5]

[0060] [Composition Examples 6-1 to 6-5] (Hair gel manufacturing) A gel was prepared from the ingredients listed in Table 6 below. This hair gel exhibits the effects of the present invention.

[0061] [Table 6]

[0062] [Composition Examples 7-1 to 7-5] (Hair cream manufacturing) A cream was prepared from the ingredients listed in Table 7 below. This hair cream exhibits the effects of the present invention.

[0063] [Table 7] [Industrial Applicability]

[0064] The composition of the present invention can be used as a hair growth agent and is therefore industrially useful.

Claims

1. A composition for the scalp and / or hair, comprising one or more selected from black currant, rooibos and Swertia japonica.

2. The composition according to claim 1, characterized in that it is a hair growth composition.

3. The composition according to claim 1, which is a composition for promoting COL17A1 expression.

4. The composition according to any one of claims 1 to 3, which is an external preparation.

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