Microbial control material, microbial control composition, and method for using microbial control material

Abstract

To provide a microbial control material capable of controlling both the inhibition and the promotion of microbial growth.SOLUTION: A microbial control material comprises an alloy material, wherein the alloy material comprises magnesium as a main component.SELECTED DRAWING: Figure 1

Description

Technical Field

[0001] The present invention relates to a microbial control material, a microbial control composition, and a method for using the microbial control material.

Background Art

[0002] Currently, various microorganisms exist in nature. Examples of such microorganisms include harmful microorganisms that exhibit pathogenicity in plant cultivation, beneficial microorganisms that exhibit usefulness in food processing, and the like. Microorganisms exhibit various properties depending on their types. For example, most plant diseases are infectious diseases caused mainly by microorganisms. Therefore, techniques for controlling infectious diseases caused by such microorganisms have been developed.

[0003] For example, Patent Document 1 describes a composition for controlling soil-borne diseases of plants, which is characterized by containing a combination of (1) at least one selected from calcium carbonate, magnesium carbonate, and magnesium silicate, (2) trace elements, and (3) at least one selected from silicon dioxide and iron oxide, namely, a combination of (1) and (2), a combination of (1) and (3), or a combination of (1), (2), and (3).

Prior Art Documents

Patent Documents

[0004]

Patent Document 1

Summary of the Invention

Problems to be Solved by the Invention

[0005] As described above, it is known that there are harmful microorganisms that cause damage to humans and beneficial microorganisms that bring benefits to humans. Examples of harmful microorganisms include microorganisms that become pathogenic bacteria when cultivating plants. For such harmful microorganisms, means for suppressing their growth are required. For example, hydroponics is known as a conventionally well-known cultivation method. Hydroponics is widely used in protected horticulture because it is less affected by climate change and can obtain stable yields. Hydroponics is a cultivation method that supplies plants with the fertilizer components necessary for growth by means of a culture solution without using soil, and has the advantage of being easy to avoid diseases because plants are cultivated in a closed environment. However, hydroponics has the disadvantage that once a disease occurs, the damage is likely to be great. Therefore, in applications such as hydroponics, means for suppressing the growth of pathogenic bacteria are particularly required. Examples of beneficial microorganisms include microorganisms that can promote the growth of plants (such as vegetables) and fungi (such as mushrooms) that have high edible value for humans. For such beneficial microorganisms, means for promoting growth are required.

[0006] The problem to be solved by the present invention is to provide a microorganism control material, a microorganism control composition, and a method for using the microorganism control material that can control the suppression and promotion of the growth of microorganisms.

Means for Solving the Problem

[0007] The present invention includes the following aspects. <1>A microorganism control material containing an alloy material, wherein the alloy material contains magnesium as a main component. <2>The microorganism control material according to <1>, wherein the alloy material further contains at least one element selected from the group consisting of zinc, calcium, boron, silicon, phosphorus, sulfur, potassium, manganese, iron, nickel, copper, molybdenum, and silver. <3>The microorganism control material according to <2>, wherein the content of the element is 0.1% by mass to 20% by mass in total with respect to the total mass of the alloy material. <4>The microorganism control material according to any one of <1> to <3>, wherein the alloy material further contains aluminum. <5>A microorganism control composition containing ions derived from an alloy material and a liquid, wherein the alloy material contains magnesium as a main component. <6>The microbial control composition according to <5>, wherein the alloy material further contains at least one element selected from the group consisting of zinc, calcium, boron, silicon, phosphorus, sulfur, potassium, manganese, iron, nickel, copper, molybdenum, and silver. <7>A method for using a microbial control material, comprising an ion generation step of bringing a microbial control material containing an alloy material and having magnesium as a main component in the alloy material into contact with a liquid and leaving it to stand, thereby generating ions derived from the alloy material.

Effects of the Invention

[0008] According to the present invention, it is possible to provide a microbial control material, a microbial control composition, and a method for using a microbial control material that can control the suppression and promotion of the growth of microorganisms.

Brief Description of the Drawings

[0009]

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Mode for Carrying Out the Invention

[0010] Hereinafter, the mode for carrying out the present invention (hereinafter referred to as "the present embodiment") will be described in detail. It should be noted that the following present embodiment is an exemplification for explaining the present invention, and the present invention is not limited to the following embodiment. The present invention can be appropriately modified and implemented within the scope of its gist. In the present embodiment, the numerical range indicated by "~" means a range including the numerical values described before and after "~" as the minimum value and the maximum value, respectively. In the numerical ranges described stepwise in the present embodiment, the upper limit value or the lower limit value described in a certain numerical range may be replaced with the upper limit value or the lower limit value of the numerical ranges described in other stepwise descriptions. Also, in the numerical ranges described in the present disclosure, the upper limit value or the lower limit value described in a certain numerical range may be replaced with the value shown in the examples. In the present embodiment, a combination of two or more preferred embodiments is a more preferred embodiment. In the present embodiment, the amount of each component means the total amount of a plurality of substances corresponding to each component, unless otherwise specified, when there are a plurality of substances corresponding to each component.

[0011] ≪Microorganism control material≫ The microorganism control material of the present embodiment contains an alloy material, and the alloy material contains magnesium as a main component. The alloy material containing magnesium as a main component means an alloy material in which the magnesium content is 80% by mass or more based on the total mass of the alloy material. The form of the microorganism control material of the present embodiment includes solids and liquids. In the present embodiment, microorganism control means expressing preferable traits and growth patterns by changing the metabolism of microorganisms, that is, promoting or inhibiting the growth of microorganisms and controlling their metabolism. Microorganism control means, for example, showing an inhibitory effect on the growth of harmful bacteria such as plant pathogenic bacteria and a promoting effect on the growth of beneficial bacteria. In the present embodiment, the alloy material consists of a plurality of metal elements or consists of metal elements and non-metal elements. The alloy material preferably consists essentially of metal elements. "Consisting essentially of metal elements" means that the total content of metal elements may be 90% by mass or more, preferably 95% by mass or more, more preferably 98% by mass or more based on the total mass of the alloy material.

[0012] The alloy material in the microorganism control material of the present embodiment can release magnesium ions, which are ions of magnesium as the main component. These magnesium ions are preferably released when the alloy material is exposed to a liquid (for example, water) or a high-humidity environment. More specifically, when the alloy material is exposed to a liquid or a high-humidity environment, the alloy material itself dissolves and magnesium ions are preferably released. The reason why the microbial control material of this embodiment exhibits the effect of microbial control is presumably that ions of various elements constituting the alloy material are released by the dissolution of the alloy material itself, and at least a part of the released ions acts on microorganisms.

[0013] The microbial control material of this embodiment is preferably made of the above alloy material.

[0014] <Alloy material> (Magnesium) The alloy material in this embodiment contains magnesium as a main component. The alloy material in this embodiment releases magnesium ions when exposed to a liquid (e.g., water) or a high-humidity environment. The content of magnesium is preferably 80% by mass to 99% by mass, more preferably 85% by mass to 99% by mass, and even more preferably 90% by mass to 98% by mass with respect to the total mass of the alloy material.

[0015] (Aluminum) The alloy material preferably further contains aluminum. By further containing aluminum, the alloy material has excellent corrosion resistance. That is, since the dissolution rate of the alloy material can be moderated, the release rate of ions from the alloy material can be suppressed. As a result, the alloy material has excellent sustained-release properties of ions by further containing aluminum. In this embodiment, even when the alloy material is exposed to a liquid or a high-humidity environment, aluminum does not elute at all or hardly elutes, and substantially no aluminum ions are released.

[0016] The content of aluminum is preferably 0.1% by mass to 10.0% by mass, more preferably 0.3% by mass to 3.0% by mass, and even more preferably 0.5% by mass to 1.0% by mass with respect to the total mass of the alloy material.

[0017] (Other elements) The alloy material in this embodiment preferably further contains elements other than magnesium and aluminum. As the other elements, the alloy material preferably further contains at least one element (also referred to as an element of the sub-component) selected from the group consisting of zinc, calcium, boron, silicon, phosphorus, sulfur, potassium, manganese, iron, nickel, copper, molybdenum, and silver. More preferably, the alloy material further contains at least one element selected from the group consisting of zinc and calcium, and even more preferably, it contains zinc and calcium. By including the above elements as elements other than magnesium and aluminum, the alloy material can better control the inhibition and promotion of the growth of microorganisms.

[0018] The total content of the above elements of the sub-component is preferably 0.1% by mass to 20% by mass, more preferably 1% by mass to 15% by mass, and even more preferably 3% by mass to 10% by mass, based on the total mass of the alloy material.

[0019] When the alloy material contains zinc, the content of zinc is preferably 0.1% by mass to 15% by mass, more preferably 1% by mass to 10% by mass, and even more preferably 3% by mass to 6% by mass, based on the total mass of the alloy material.

[0020] When the alloy material contains calcium, the content of calcium is preferably 0.1% by mass to 3% by mass, more preferably 0.3% by mass to 2% by mass, and even more preferably 0.5% by mass to 1.5% by mass, based on the total mass of the alloy material.

[0021] Examples of the method for manufacturing the alloy material include a method of producing an alloy ingot from raw materials such as magnesium, zinc, aluminum, and calcium, and then processing the ingot into a desired shape. Specifically, pure metals of various alloying elements are placed in a graphite crucible for melting so as to achieve a desired mass ratio. This graphite crucible for melting is placed inside a high-frequency coil within a high-frequency melting furnace chamber, and a mold is installed in front of the high-frequency coil. Then, after evacuating the inside of the high-frequency melting furnace chamber, it is filled with helium gas, and the graphite crucible is heated to 750 °C or higher. After confirming that the metal has completely melted, the molten alloy in the graphite crucible is poured into a copper mold, and after cooling, an alloy ingot is obtained from the mold.

[0022] Next, an extrusion billet cut out from the obtained alloy ingot ingot is subjected to primary processing (hot extrusion) under processing conditions of a processing temperature of 400 °C, a processing speed of 300 mm / min, and an extrusion ratio of 10. After heating the billet after the primary processing in an air furnace, it is passed through a rolling roll heated by a built-in heater to perform secondary processing (hot rolling) to obtain an alloy plate. After cutting the obtained alloy plate with a cutting grindstone, it is polished using abrasive paper to obtain a plate-shaped alloy material.

[0023] ≪Applications≫ The microbial control material of the present embodiment is suitably used for cultivation. Examples of cultivation methods include soil cultivation, hydroponics, and mushroom bed cultivation. Among these, the microbial control material of the present embodiment is suitably used for hydroponics. Hydroponics is a cultivation method in which a culture solution containing nutrients and water is supplied to plants and does not require soil. The microbial control material of the present embodiment can be used against microorganisms (bacteria) generated in the above cultivation. For example, when the microorganism is a harmful bacterium such as a pathogen against plants, its growth can be suppressed, and when the microorganism is a beneficial bacterium, its growth can be promoted.

[0024] <Microorganisms> The microorganisms targeted in the present embodiment are not particularly limited, and examples include protists, fungi, and bacteria. Examples of protists include oomycetes. Examples of the fungus include Ascomycetes, Basidiomycetes, Mucorales, etc. Examples of the bacteria include Actinobacteria, Proteobacteria, etc.

[0025] Examples of the microorganisms targeted in this embodiment include, for example, the genus Achlya such as americana, flagellata, klebsiana; the genus Acholeplasma such as laidlawii; the genus Agrobacterium such as tumefaciens; the genus Alternaria such as alternata; the genus Aphanomyces such as cochlioides, raphani; the genus Aspergillus such as brasiliensis, flavus, niger, penicilloides, terreus, tonophilum, tubingensis, versicolor; the genus Aureobasidium such as pullulans; the genus Bacillus such as atrophaeus, cereus, subtilis; bacteriophages such as MS2, Q-beta, φX174; the genus Botryosporium such as pulchrum; the genus Botrytis such as cinerea; the genus Brevundimonas such as diminuta; the genus Burkholderia such as ambifaria, andropogonis, caryophylli, cenocepacia, cepacia, gladioli, glumae, plantarii, pyrrocinia; the genus Calonectria such as ilicicola; the genus Candida such as albicans; the genus Ceratobasidium such as cornigerum, setariae; the genus Ceratocystis such as fimbriata; the genus Chaetomium such as globosum; the genus Choanephora such as cucurbitarum; the genus Ciborinia such as allii, gentianae; the genus Cladosporium such as cladosporioides, sphaerospermum;Genus Clavibacter such as michiganensis; Genus Clostridium such as sporogenes; Genus Colletotrichum such as coccodes; Genus Corynespora such as cassiicola; Genus Curtobacterium such as flaccumfaciens; Genus Cylindrocarpon such as destructans, obtusisporum; Genus Cylindrocladium such as floridanum; Genus Diaporthe such as batatas, destruens; Genus Dickeya such as dianthicola, zeae; Genus Dictyuchus such as sterilis; Genus Didymella such as bryoniae; Genus Elsinoe such as araliae; Genus Erwinia such as persicina, rhapontici; Genus Escherichia such as coli; Genus Fomitopsis such as palustris; Genus Fusarium such as avenaceum, buharicum, commune, languescens, moniliforme, oxysporum, proliferatum, roseum, solani, solani-melongenae, subglutinans, torulosum, tricinctum; Genus Gaeumannomyces such as graminis; Genus Geobacillus such as stearothermophilus; Genus Gibberella such as fujikuroi, zeae; Genus Helicobasidium such as mompa; Genus Helicoceras such as oryzae; Genus Helminthosporium such as sigmoideum; Genus Hormoconis such as resinae; Genus Klebsiella such as pneumoniae;Genus Kocuria such as rhizophila; Genus Lactobacillus such as acidophilus, fructivorans, hilgardii, paracasei; Genus Macrophomina such as phaseolina; Genus Magnaporthe such as salvinii; Genus Methylorubrum such as extorquens; Genus Micrococcus such as luteus; Genus Monilochaetes such as infuscans; Genus Monosporascus such as cannonballus; Genus Mucor such as fragilis; Genus Mycobacterium such as smegmatis; Genus Mycocentrospora such as acerina; Mycoplasma such as arginini, fermentans, hyorhinis, orale, pneumoniae, salivarium; Genus Myrothecium such as verrucaria; Genus Nodulisporium such as melonis; Genus Ophionectria such as sojae; Genus Paecilomyces such as variotii; Genus Pantoea such as ananatis; Genus Pectobacterium such as carotovorum; Genus Penicillium such as brevicompactum, citrinum, cyclopium, expansum, funiculosum, guanacastense, ochrochloron, pinophilum, piscarium, sclerotiorum, simplicissimum; Genus Phoma such as lingam, wasabiae; Genus Phomopsis such as rojana;Genus Phytophthora such as cactorum, cambivora, capsici, chrysanthemi, cryptogea, fragariae, fragariaefolia, gloveri, hedraiandra, infestans, japonica, katsurae, melonis, multivesiculata, nicotianae, palmivora, porri, sojae, syringae; Genus Candidatus Phytoplasma; Genus Phytopythium such as helicoides; Genus Plasmodiophora such as brassicae; Genus Plectosphaerella; Genus Plectosporium such as tabacinum; Genus Pseudomonas such as aegrilactucae, aeruginosa, allii, cannabina, cichorii, corrugata, fluorescens, kitaguniensis, lactucae, marginalis, morbosilactucae, paraeruginosa, protegens, putida, savastanoi, syringae, viridiflava; Genus Pyrenochaeta such as gentianicola, lycopersici, terrestris; Genus Pythiomorpha such as miyabeana, oryzae; Genus Pythium such as aphanidermatum, cryptoirregulare, cucurbitacearum, debaryanum, deliense, dissotocum, irregulare, mastophorum, megalacanthum, myriotylum, paroecandrum, recalcitrans, scleroteichum, spinosum, splendens, sulcatum, sylvaticum, ultimum, vexans, volutum, zingiberis;Genus Ralstonia such as pseudosolanacearum and solanacearum; Genus Ramularia such as petuniae; Genus Rhizobacter such as dauci; Genus Rhizobium such as radiobacter, rhizogenes, and vitis; Genus Rhizoctonia such as fragariae, solani, and tuliparum; Genus Rhizopus such as arrhizus, chinensis, javanicus, koreanus, oryzae, stolonifer, and tritici; Genus Rhodococcus such as equi; Genus Rosellinia such as necatrix; Genus Salmonella such as enterica subsp. Enterica serovar Abony and enterica subsp. Enterica serovar Typhimurium; Genus Sclerotinia such as intermedia, minor, and sclerotiorum; Genus Sclerotium such as cepivorum, hydrophilum, and rolfsii; Genus Scopulariopsis such as brevicaulis; Genus Serratia such as marcescens Sphaceloma genus; Genus Staphylococcus such as aureus, aureus subsp., and epidermidis; Genus Streptomyces such as ipomoeae and scabies; Genus Talaromyces such as funiculosus and pinophilus; Genus Thanatephorus such as cucumeris; Genus Thielaviopsis such as basicola and thielavioides; Genus Trametes such as versicolor; Genus Trichoderma such as hamatum, harzianum, virens, and viride;Microorganisms such as Trichophyton spp. including mentagrophytes; Trichosporo spp.; Urocystis spp. including cepulae, magica; Verticillium spp. including albo-atrum, dahliae, longisporum, nigrescens; Villosiclava virens; Waitea circinata; Wallemia sebi; Xanthomonas axonopodis, campestris, cucurbitae, euvesicatoria, fragariae, hortorum, oryzae, vesicatoria, etc. are mentioned.

[0026] As described above, the microbial control material of the present embodiment exhibits a growth inhibitory effect on harmful microorganisms and a growth promoting effect on beneficial microorganisms. Examples of beneficial microorganisms include, for example, Acetobacter aceti, Acetobacter orientalis, Acetobacter pasteurianus, Acetobacter xylinum, Agaricus bisporus, Armillaria mellea, Aspergillus luchuensis, Aspergillus oryzae, Aspergillus sojae, Aspergillus terreus, Aurantiochytrium sp., Bacillus amyloliquefaciens, Bacillus subtilis, Bifidobacterium bifidum, Botryococcus braunii, Bradyrhizobium japonicum, Chlamydomonas reinhardtii, Chlorella sp., Corynebacterium ammoniagenes, Corynebacterium glutamicum, Desmodesmus sp., Enterococcus faecium, Euglena sp., Flammulina velutipes, Fusarium sp.(Fusarium sp), Ganoderma lucidum, Gluconacetobacter diazotrophicus, Gluconacetobacter hansenii, Gluconacetobacter kombuchae, Gluconacetobacter xylinus, Gluconobacter albidus, Heteroconium chaetospira, Kluyveromyces marxianus, Kluyveromyces lactis, Lactobacillus acidophilus, Lactobacillus bulgaricus, Lactobacillus casei, Lactobacillus gasseri, Lactobacillus paracasei, Lactobacillus plantarum, Lactococcus cremoris, Lactococcus lactis, Lentinula edodes, Leuconostoc mesenteroides, Mesorhizobium loti, Methanobacterium thermoautotrophicum, Methanosarcina barkeri, Nannochloropsis sp.) Pediococcus damnosus, Peniophora cinerea, (Phlebia sp.), Pholiota nameko, Pleurotus ostreatus, Pseudochoricystis ellipsoidea, Rhizophagus irregularis, Rhizopus oryzae, Saccharomyces cerevisiae, Saccharopolyspora erythraea, Scenedesmus sp., Schizosaccharomyces pombe, Streptococcus thermophilus, Streptomyces griseus, Synechococcus elongatus, Tolypocladium inflatum, Trametes suaveolens, Trichoderma hamatum, Trichoderma harzianum, Trichoderma virens, Tricholoma matsutake, Zygosaccharomyces rouxii, etc. can be mentioned. Examples of harmful microorganisms include, for example, Aspergillus flavus, Aspergillus fumigatus, Aspergillus ochraceus, Bacillus cereus, Botrytis cinerea, Burkholderia glumae, (Enterococcus faecalis), Enterococcus faecium, Ralstonia pseudosolanacearum, Streptomyces scabies, Cladosporium halotolerans, Clostridium perfringens, Escherichia coli, Fusarium graminearum, Lacticaseibacillus rhamnosus, Lactobacillus fructivorans, Lactobacillus hilgardii, Penicillium expansum, Streptomyces scabies, and the like.

[0027] <Plant> The plants targeted in this embodiment are not particularly limited. For example, melon, pumpkin, cucumber, watermelon, snake gourd, winter melon, morning glory, pepper, tomato, eggplant, cauliflower, cabbage, komatsuna, Chinese cabbage, hakusai, broccoli, turnip, daikon radish, wasabi, aralia, butterbur, lettuce, celery, parsley, strawberry, asparagus, onion, garlic chives, leek, sweet potato, ginger, carrot, rice, broad bean, soybean, okra, spinach, chrysanthemum, petunia, carnation, tulip, cymbidium, Turkish aster, gentian, persimmon, loquat, fig, apricot, Japanese apricot, cherry, apple, grape, chestnut, kiwifruit, citrus, etc. can be mentioned.

[0028] <Infectious disease> There are no particular restrictions on the infectious diseases to which this embodiment is applicable. For example, melon vine blight, melon basal rot, melon mosaic disease, melon brown spot bacterial disease, melon gummy stem blight, melon soft rot, melon spot bacterial disease, melon root rot, melon phytophthora blight, melon sclerotinia rot, melon red root rot, melon black dot root rot, melon southern blight, melon damping-off, melon vine wilt, melon seedling damping-off, melon root rot, melon root rot and wilt, melon half wilt, pumpkin bacterial wilt, pumpkin brown spot bacterial disease, pumpkin spot bacterial disease, pumpkin phytophthora blight, pumpkin southern blight, pumpkin damping-off, pumpkin vine wilt, cucumber green mottle mosaic virus, cucumber bacterial wilt, cucumber brown spot bacterial disease, cucumber soft rot, cucumber spot bacterial disease, cucumber phytophthora blight, cucumber brown spot, cucumber sclerotinia rot, cucumber southern blight, cucumber vine wilt, cucumber vine blight, cucumber seedling damping-off, cucumber root rot, cucumber gray mold, cucumber half wilt, cucumber homopsis root rot, cucumber purple root rot, watermelon green mottle mosaic virus, watermelon bacterial wilt, watermelon brown spot bacterial disease, watermelon phytophthora blight, watermelon sclerotinia rot, watermelon black dot root rot, watermelon southern blight, watermelon damping-off, watermelon vine wilt, watermelon vine blight, watermelon half wilt, watermelon fusarium damping-off, tuberous begonia spot bacterial disease, eggplant damping-off, eggplant vine wilt, eggplant vine blight, yucca brown spot bacterial disease, yucca spot bacterial disease, yucca black dot root rot, yucca southern blight, yucca vine wilt, yucca vine blight, yucca seedling damping-off, yucca gray mold, capsicum bacterial wilt, capsicum damping-off, capsicum soft rot, capsicum spot bacterial disease, capsicum wilt, capsicum phytophthora blight, capsicum sclerotinia rot, capsicum black dot root rot, capsicum southern blight, capsicum damping-off, capsicum seedling damping-off, capsicum half wilt, tomato streak virus, tomato mosaic virus, tomato bacterial wilt, tomato damping-off, tomato stem rot bacterial disease, tomato black spot bacterial disease, tomato soft rot, tomato spot bacterial disease, tomato leaf spot bacterial disease, tomato rot, tomato alternaria stem blight, tomato wilt, tomato phytophthora blight, tomato brown root rot, tomato brown rot, tomato sclerotinia rot, tomato red root rot, tomato black dot root rot, tomato small sclerotinia rot, tomato southern blight, tomato seedling damping-off, tomato root rot, tomato root rot and wilt, tomato root rot and phytophthora blight, tomato gray moldTomato half-wilt disease, eggplant mosaic disease, eggplant bacterial wilt, eggplant brown spot bacterial disease, eggplant stem canker bacterial disease, eggplant stem rot bacterial disease, eggplant soft rot, eggplant spot bacterial disease, eggplant blight, eggplant brown rot, eggplant sclerotinia disease, eggplant black root rot, eggplant southern blight, eggplant damping-off, eggplant root rot blight, eggplant semi-wilt, tomato half-wilt disease, cauliflower black rot, cauliflower black spot bacterial disease, cauliflower soft rot, cauliflower chlorosis, cauliflower root knot disease, cabbage black rot, cabbage black spot bacterial disease, cabbage soft rot, cabbage chlorosis, cabbage stem rot, cabbage sclerotinia disease, cabbage root rot, cabbage root knot disease, cabbage Verticillium wilt, cabbage damping-off, komatsuna chlorosis, turnip green spot bacterial disease, turnip chlorosis, Chinese cabbage black rot, Chinese cabbage black spot bacterial disease, Chinese cabbage soft rot, Chinese cabbage rot, Chinese cabbage yellowing, Chinese cabbage sclerotinia disease, Chinese cabbage bottom rot, Chinese cabbage root constriction disease, Chinese cabbage root knot disease, Chinese cabbage Pythium rot, broccoli Pythium rot, radish bacterial wilt, radish black rot, radish black spot ring rot, radish black spot bacterial disease, radish downy mildew, radish soft rot, radish chlorosis, radish circular brown spot disease, radish sclerotinia disease, radish black spot disease, radish root rot, radish root knot disease, radish leaf rot, radish Verticillium black spot disease, radish rot, radish damping-off, wasabi sclerotinia disease, wasabi stem rot, wasabi black spot disease, wasabi root knot disease, aralia chlorosis, aralia wilt, aralia blight, aralia sclerotinia disease, aralia southern blight, aralia downy mildew, chrysanthemum bacterial wilt, chrysanthemum black rot, chrysanthemum rot, chrysanthemum wilt, chrysanthemum sclerotinia disease, lettuce soft rot, lettuce spot bacterial disease, lettuce rot, lettuce sclerotinia disease, lettuce small sclerotinia disease, lettuce basal rot, lettuce root rot, celery soft rot, celery spot bacterial disease, celery leaf blight bacterial disease, celery rot, celery chlorosis, celery sclerotinia disease, parsley soft rot, parsley wilt, parsley blight, parsley damping-off, parsley seedling damping-off, parsley root rot, parsley root constriction disease, strawberry angular leaf spot bacterial disease, strawberry chlorosis, strawberry wilt, strawberry blight, strawberry fruit rot, strawberry sclerotinia disease, strawberry black root rot, strawberry southern blight, strawberry soft rot, strawberry root rot, strawberry bud blight, asparagus brown sclerotinia root rot,Asparagus stem rot, asparagus white root rot, asparagus damping-off, asparagus seedling damping-off, asparagus purple root rot, onion blight, onion soft rot, onion bacterial leaf spot, onion rot, onion scale rot, onion downy mildew, onion dry rot, onion sclerotinia, onion black mold, onion black rot sclerotinia, onion smut, onion red root rot, onion microsclerotinia, onion southern blight, onion white blight, onion seedling damping-off, Welsh onion stem rot bacteriosis, Welsh onion soft rot, Welsh onion dry rot, Welsh onion black rot sclerotinia, Welsh onion red root rot, Welsh onion southern blight, Welsh onion leaf rot, leek streak disease, leek soft rot, leek bacterial leaf spot, leek rot, leek wilt, leek downy mildew, leek black rot sclerotinia, leek smut, leek red root rot, leek microsclerotinia, leek southern blight, leek white blight, leek seedling damping-off, sweet potato damping-off, sweet potato blue mold, sweet potato blight, sweet potato brown dry rot, sweet potato dry rot, sweet potato basal rot, sweet potato sclerotinia, sweet potato black spot, sweet potato black spot, sweet potato microsclerotinia, sweet potato southern blight, sweet potato white rot, sweet potato white root rot, sweet potato charcoal rot, sweet potato vine rot, sweet potato soft rot, sweet potato root rot, sweet potato gray mold, sweet potato purple root rot, ginger rot, ginger rhizome rot, ginger damping-off, ginger sheath blight, carrot knot disease, carrot crown gall disease, carrot streptomyces rot disease, carrot soft rot, carrot bacterial leaf spot, carrot chlorosis, carrot brown root rot, carrot dry rot, carrot sclerotinia, carrot black soot, carrot black root rot, carrot stain rot, carrot southern blight, carrot rot disease, carrot root rot, carrot purple root rot, rice blast, rice downy mildew, rice stem rot, rice bacterial blight, rice seedling damping-off bacteria, rice kernel rot bacteria, rice downy mildew, rice brown sclerotinia, rice brown microsclerotinia, rice brown sheath blight, rice spherical sclerotinia, rice black grain sclerotinia, rice small black sclerotinia, rice small sclerotinia, rice southern blight, rice red sclerotinia, rice damping-off, rice seedling rot, rice seedling damping-off, rice gray sclerotinia, rice foolish seedling disease, rice leaf sheath net blotch, rice sheath blight, rice cottony blight, broad bean bacterial wilt, broad bean downy mildew, broad bean sclerotinia, broad bean stem rot, broad bean black root disease, broad bean southern blight, broad bean white root rot, broad bean damping-off, broad bean root rot, soybean chlorotic mottle virus disease, soybean streak disease, soybean leaf blight, soybean bacterial leaf spot, soybean wilt, soybean stem blightSoybean sclerotinia, soybean stem blight, soybean black root rot, soybean black root rot, soybean southern blight, soybean damping-off, soybean Rhizoctonia root rot, okra blight, okra damping-off, okra half wilt, spinach mosaic virus, spinach wilt, spinach blight, spinach foot rot, spinach powdery mildew, spinach damping-off, spinach Verticillium wilt, spinach root rot, potato late blight, chrysanthemum bacterial wilt, chrysanthemum crown gall, chrysanthemum soft rot, chrysanthemum wilt, chrysanthemum blight, chrysanthemum sclerotinia, chrysanthemum southern blight, chrysanthemum damping-off, chrysanthemum half wilt, chrysanthemum stem blight, chrysanthemum white root rot, petunia sclerotinia, petunia white mold, carnation bacterial wilt, carnation bacterial damping-off, carnation leaf spot, carnation wilt, carnation blight, carnation sclerotinia, carnation stem rot, carnation neck rot, carnation southern blight, carnation damping-off, carnation root rot, tulip black rot, tulip soft rot, tulip blue mold, tulip blight, tulip bulb rot, tulip root rot, tulip sclerotinia, tulip stem blight, tulip southern blight, tulip white blight, tulip gray rot, tulip root rot, tulip rot, cymbidium brown rot, cymbidium soft rot, cymbidium blight, cymbidium brown leaf blight, cymbidium southern blight, cymbidium seedling black rot, cymbidium rot, Turkish aster bacterial wilt, Turkish aster foot rot, Turkish aster sclerotinia, Turkish aster stem rot, Turkish aster damping-off, Turkish aster root rot, gentian leaf spot, gentian brown root rot, gentian southern blight, gentian leaf rot, gentian flower rot sclerotinia, gentian knot, kaki crown gall, kaki white root rot, kaki purple root rot, kaki Homopsis damping-off, loquat white root rot, loquat microsclerotinia, fig crown gall, fig blight, fig sclerotinia, fig southern blight, fig white root rot, fig soft rot, fig purple root rot, apricot crown gall, apricot white root rot, apricot purple root rot, Japanese apricot crown gall, Japanese apricot blight, Japanese apricot sclerotinia, Japanese apricot white root rot, Japanese apricot purple root rot, quince crown gall, quince sclerotinia, quince white root rot, quince purple root rot, peach crown gall, peach white root rot, peach purple root rot, European pear blight, European pear white root rot, pear crown gall, pear blight,Apple sclerotinia rot, apple white root rot, apple purple root rot, loquat root cancer, loquat blight, loquat white root rot, loquat purple root rot, peach root cancer, peach sclerotinia rot, peach white root rot, peach purple root rot, apple root cancer, apple blight, apple southern blight, apple white root rot, apple purple root rot, grape root cancer, grape white root rot, grape half wilt, grape purple root rot, chestnut root cancer, chestnut blight, chestnut white root rot, chestnut purple root rot, kiwifruit white root rot, citrus root cancer, citrus sclerotinia rot, citrus white root rot, citrus purple root rot, citrus fusarium wilt, etc. can be mentioned.

[0029] ≪Microbial control composition≫ The microbial control composition of the present embodiment contains ions derived from an alloy material and a liquid, and the alloy material contains magnesium as a main component. The microbial control composition of the present embodiment may contain a liquid and ions obtained by bringing the microbial control material of the present embodiment into contact with the liquid. For example, by adding the microbial control material of the present embodiment to water as a liquid and leaving it for a predetermined time, magnesium ions and the like in the alloy material elute into the water, and ions derived from the alloy material such as magnesium ions are generated in the water. Therefore, the microbial control composition of the present embodiment may further contain the microbial control material of the present embodiment. After that, the microbial control material may be taken out after sufficient ions are generated. In this case, the microbial control composition of the present embodiment does not contain the microbial control material of the present embodiment.

[0030] In the microbial control composition of the present embodiment, in the same manner as in the case of the microbial control material of the present embodiment, details such as specific embodiments and preferred embodiments of each configuration described in the above-mentioned ≪Microbial control material≫ can be appropriately applied.

[0031] In the microbial control composition of the present embodiment, in the same manner as the description of the above-mentioned (other elements), it is preferable that the alloy material further contains at least one element selected from the group consisting of zinc, calcium, boron, silicon, phosphorus, sulfur, potassium, manganese, iron, nickel, copper, molybdenum, and silver.

[0032] ≪Method for Using Microorganism Control Material≫ The method for using the microorganism control material of the present embodiment includes an ion generation step of bringing a microorganism control material containing an alloy material and having magnesium as a main component of the alloy material into contact with a liquid and leaving it to generate ions derived from the alloy material. As the liquid, for example, water can be used. The method for using the microorganism control material of the present embodiment may be carried out using the microorganism control material of the present embodiment. Therefore, as the "microorganism control material containing an alloy material and having magnesium as a main component of the alloy material", details such as specific embodiments and preferred embodiments of each configuration described in the above-mentioned ≪Microorganism Control Material≫ can be appropriately applied.

[0033] In the ion generation step, the standing time is preferably 30 minutes or more, more preferably 3 hours or more, and even more preferably 12 hours or more. When the standing time is within the above range, ions are generated well, so the microorganism control effect is excellent. In the ion generation step, the upper limit of the standing time is not particularly limited. In the ion generation step, the standing time may be 2 years or less, 1 year or less, or 6 months or less.

[0034] ≪Use≫ The microorganism control material of the present embodiment can be used for the purpose of controlling the suppression and promotion of the growth of microorganisms. For example, the microorganism control material of the present embodiment may be used to suppress the growth of pathogenic bacteria in applications such as hydroponics. Also, for example, the microorganism control material of the present embodiment may be used to promote the growth of beneficial microorganisms. Examples of beneficial microorganisms include microorganisms that can promote the growth of plants (such as vegetables) and fungi (such as mushrooms) that have high edible value for humans.

[0035] The microbial control material of this embodiment can be widely applied to various uses for the purpose of controlling the suppression and promotion of the growth of microorganisms. More specific uses are as follows. The microbial control material of this embodiment can be used for edible or medicinal mushrooms. Thereby, edible or medicinal mushrooms can be cultivated in large quantities and rapidly. There are no particular restrictions on edible mushrooms, and examples include shiitake mushrooms, matsutake mushrooms, and eringi mushrooms. There are no particular restrictions on medicinal mushrooms, and examples include ganoderma lucidum.

[0036] By using the microbial control material of this embodiment for culturing molds, the production of metabolite of molds that can be used for antibiotics, hormones, etc. can be promoted.

[0037] By using the microbial control material of this embodiment for fermentation technology, the efficiency of fermentation technology can be promoted. For example, by using the microbial control material of this embodiment for fermentation technology, the productivity of fermented foods and fermented beverages can be improved. Also, for example, the microbial control material of this embodiment can improve the treatment efficiency of biomass. Examples of biomass include waste-based biomass (food waste, livestock excrement, etc.), unused biomass (agricultural waste, construction waste, forest residue, etc.), and energy crops (sugarcane, corn, etc.). Furthermore, for example, the microbial control material of this embodiment contributes to resource development by promoting it through fermentation. Examples of resources include biofuels such as ethanol and methane gas; biodegradable plastics such as polylactic acid; etc.

Examples

[0038] Hereinafter, specific examples and comparative examples will be given to explain this embodiment in more detail, but the present invention is not limited by the following examples and comparative examples. In each drawing, "▲" indicates spores and "△" indicates hyphae.

[0039] In this example, OD600 was measured using a spectrophotometer (Thermo Scietific Genesys 40). In this example, pH was measured using a pH meter (HORIBA LAQUAtwin-pH033).

[0040] <Experiment 1> Elution verification of microbial control material (Example 1-1) [Preparation of microbial control material] A microbial control material was prepared according to each step described in FIG. 1. FIG. 1 is a flowchart for preparing the microbial control material. Specifically, first, pure metals of alloying elements magnesium (Mg: 99.9 mass%), zinc (Zn: 99.99 mass%), aluminum (Al: 99.99 mass%), and calcium (Ca: 99.99 mass%) were placed in a graphite crucible (Φ60×L145 mm) for melting so that the mass ratios were Mg 93.2%, Zn 5.0%, Al 1.0%, and Ca 0.8%. Thereafter, this graphite crucible for melting was placed inside the high-frequency coil in the high-frequency melting furnace chamber, and a copper mold (Φ60×L145 mm) was installed in front of the high-frequency coil. Then, after evacuating the inside of the high-frequency melting furnace chamber, it was filled with helium gas, and the graphite crucible was heated to 750°C or higher. After confirming that the metal was completely melted and holding for 5 minutes, the molten alloy in the graphite crucible was poured into the copper mold, and after cooling, an alloy ingot was obtained from the mold.

[0041] Next, an extrusion billet of Φ60 mm×L50 mm cut out from the obtained alloy ingot ingot was subjected to primary processing (hot extrusion) into a rectangular shape of W30 mm×T10 mm under processing conditions of a processing temperature of 400°C, a processing speed of 300 mm / min, and an extrusion ratio of 10, and after cutting to a length of about 100 mm, it was directly used as a billet for secondary processing. For the purpose of obtaining an alloy sheet, a billet for secondary processing was heated in an air furnace at 240°C for 30 minutes, and then passed through a rolling roll heated to 240°C by a built-in heater to perform secondary processing (hot rolling). The reduction per pass was set to 10%, and the above processing was repeated until the final plate thickness reached 1.1 mm to obtain an alloy sheet. After cutting the obtained alloy sheet into L10×W4 mm with a cutting grindstone, the back surface of the sheet was polished to a thickness of 1 mm using 1000-mesh abrasive paper to obtain a plate-shaped sample (L10×W4×T1 mm).

[0042] [Elution Ion Measurement] An immersion test was performed on the plate-shaped sample of Example 1-1, and the amount of ions eluted into the solvent was measured. First, 14 mL of distilled water was placed in a sterilized plastic 15-mL centrifuge tube, and the plate-shaped sample of the present invention that had been washed with acetone and air-dried was immersed. An immersion test was performed while rotating and shaking this at 45 rpm (revolutions per minute) with a shaker at room temperature in the air. Three weeks after the start of the immersion test, the distilled water in the centrifuge tube was collected, a liquid sample to which a metal ion detection reagent was added was prepared, and the amounts of Mg, Zn, and Ca ions eluted into the distilled water were measured with a microplate reader, and the amount of Al ions was measured by a colorimetric method. Table 1 shows the amounts of metal ions eluted from the plate-shaped sample. The elution amounts of the Mg, Zn, and Ca components, which are the alloy compositions, were 1.5±0.0 mg / dL, 3.1×10 -2 ±2.0×10 -2 mg / dL, and 1.4±0.1 mg / dL, respectively.

[0043]

Table 1

[0044] As shown in Table 1, it was found that magnesium ions, zinc ions, and calcium ions were eluted. On the other hand, aluminum ions were not substantially eluted.

[0045] <Experiment 2> Verification of the Microorganism Control Effect of the Microorganism Control Material [Preparation of the Microorganism Control Material] In the same manner as in Experiment 1, a microorganism control material was prepared according to each step described in FIG. 1. Specifically, pure metals of alloying elements magnesium (Mg: 99.9 mass%), zinc (Zn: 99.99 mass%), aluminum (Al: 99.99 mass%), and calcium (Ca: 99.99 mass%) were placed in a graphite crucible (Φ60×L145 mm) for melting so that the mass ratio was Mg 93.2%, Zn 5.0%, Al 1.0%, and Ca 0.8%. Thereafter, this graphite crucible for melting was placed inside the high-frequency coil in the high-frequency melting furnace chamber, and a copper mold (Φ60×L145 mm) was installed in front of the high-frequency coil. Then, after evacuating the inside of the high-frequency melting furnace chamber, it was filled with helium gas, and the graphite crucible was heated to 750 °C or higher. After confirming that the metal was completely melted, it was held for 5 minutes, and then the molten alloy in the graphite crucible was poured into the copper mold. After cooling, an alloy ingot was obtained from the mold.

[0046] Next, an extrusion billet of Φ60 mm×L50 mm cut out from the obtained alloy ingot ingot was subjected to primary processing (hot extrusion) into a rectangular shape of W30 mm×T10 mm under processing conditions of a processing temperature of 400 °C, a processing speed of 300 mm / min, and an extrusion ratio of 10. After cutting to a length of about 100 mm, it was directly used as a billet for secondary processing. For the purpose of obtaining an alloy plate, the billet for secondary processing was heated in an air furnace at 240 °C×30 min, and then passed through a rolling roll heated to 240 °C by a built-in heater for secondary processing (hot rolling). The reduction per pass was 10%, and the above processing was repeated until the plate thickness finally reached 1.1 mm to obtain an alloy plate. The obtained alloy plate was cut into 5 mm squares by shearing, and then the back surface of the plate was polished to a thickness of 1 mm using 1000-mesh abrasive paper to obtain a plate-shaped sample (L5×W5×T1 mm). Also, a commercially available pure magnesium sample (99.9%, about Φ3 mm) was prepared as a comparison target.

[0047] [Microbial culture test] The types and details of the microorganisms used in the microbial culture test are shown in Table 2 below.

[0048] [Table 2]

[0049] ~Growth inhibition~ [Bacteria] (Example 2-1) Burkholderia glumae (MAFF 106542, Betaproteobacteria, Gram-negative bacterium) was inoculated into 3 mL of LB medium and pre-cultured under dark conditions at 180 rpm, 30 °C for 18 hours. The main culture was inoculated with 60 μL of the pre-culture solution into 60 mL of 1 / 3 LB medium and carried out under dark conditions at 180 rpm, 30 °C for 72 hours. In the main culture, the microorganisms were cultured in the presence of the microbial control material prepared by the process of Fig. 1, and the control effect was evaluated. Three test groups were set: an untreated group, a pure magnesium group, and an alloy group. No metal material was added to the untreated group, 1 pure magnesium sample was added to the pure magnesium group, and 1 plate-shaped alloy sample was added to the alloy group to the medium, respectively. The culture was performed in 3 replicates. In the culture of the alloy group, the microbial control material (alloy) was added to the medium containing moisture, and the microbial control effect was evaluated while generating ions derived from the alloy material. Table 3 shows the results of measuring the turbidity of the bacterial cells (hereinafter also referred to as OD600) and pH of the culture solution over time. Also, Figs. 2A and 2B show graphs representing the time-dependent changes in OD600 and pH of each treatment group.

[0050] [Table 3]

[0051] As shown in Table 3, the alloy of the example had a low OD600 after 72 hours and was able to suppress the growth of microorganisms. In contrast, for pure magnesium and the untreated case, the OD600 after 72 hours was high and the growth of microorganisms could not be suppressed. The bacteria of Example 2-1 grow easily from weakly acidic to neutral, but the alloy of the example was able to suppress the growth of microorganisms more effectively than pure magnesium, even though the pH was lower than that of pure magnesium, creating an environment where bacteria grow easily.

[0052] (Example 2-2) Streptomyces scabies (MAFF 225024, actinobacterium, Gram-positive bacterium) was inoculated into YSA medium, and agar pieces (28.6 mm2, average mass 0.047 g (wet weight)) precultured in the dark at 25°C for 49 days were inoculated into 50 mL of LB medium. The culture was carried out in the dark at 25°C and 100 rpm for 7 days, and 0.5 mL of the culture solution was sampled at regular intervals to measure the pH. The cells after 7-day culture were filtered and collected using Miracloth (Merck KGaA, 475855), and the filtered cells were placed on filter paper and dried at 65°C overnight. The mass of the dried cells was measured using an electronic balance. In the main culture, microorganisms were cultured in the presence of the microbial control material prepared by the process shown in Fig. 1, and the control effect was evaluated. As shown in Table 4, there were three test groups. No metal material was added to the untreated group, one pure magnesium sample was added to the pure magnesium group, and one plate-shaped alloy sample was added to the magnesium alloy group. The culture was carried out in duplicate. In the culture of the alloy group, microorganisms were cultured while generating ions derived from the alloy material in the presence of moisture and the microbial control material (alloy). Table 4 shows the measurement results of the dried cell mass after 7 days and the results of measuring the pH of the culture solution over time. Also, Fig. 3A shows a graph representing the comparison of the dried cell mass after 7 days for each treatment group, and Fig. 3B shows a graph representing the temporal change in pH.

[0053]

Table 4

[0054] As shown in Table 4, for the alloy of the example, the value of the bacterial cell mass after 7 days was small, indicating that the growth of microorganisms could be suppressed. In contrast, for pure magnesium and the untreated case, the value of the bacterial cell mass after 7 days was large, and the growth of microorganisms could not be suppressed. The bacteria of Example 2-2 are alkaline and tend to grow. However, although the alloy of the example had a higher pH than pure magnesium and was an environment in which bacteria tended to grow, it was able to suppress the growth of microorganisms more effectively than pure magnesium.

[0055] <Fungi (filamentous fungi)> (Example 2-3) Botrytis cinerea (MAFF 237695, Ascomycetes) was inoculated onto a PDA medium, and an agar piece (28.6 mm2, mass 0.068 g (wet weight)) cultured in the dark at 25°C for 7 days was inoculated into 50 mL of a PDB medium. The main culture was carried out in the dark at 25°C and 100 rpm for 7 days, and 0.5 mL of the culture solution was sampled over time to measure the pH. The bacterial cells after 7 days of culture were filtered and collected using Miracloth (manufactured by Merck KGaA, 475855), and the filtered bacterial cells were placed on filter paper and dried at 65°C overnight. The mass of the dried bacterial cells was measured using an electronic balance. In the main culture, microorganisms were cultured in the presence of the microbial control material prepared by the process shown in FIG. 1, and the control effect was evaluated. As shown in Table 5, there were three test groups. No metal material was added to the untreated group, one pure magnesium sample was added to the pure magnesium group, and one plate-shaped alloy sample was added to the magnesium alloy group. The culture was performed in triplicate. In the culture of the alloy group, microorganisms were cultured while generating ions derived from the alloy material in the presence of moisture and the microbial control material (alloy). Table 5 shows the measurement results of the dry bacterial cell mass after 7 days and the results of measuring the pH of the culture solution over time. Also, FIG. 4A shows a graph representing the comparison of the dry bacterial cell mass after 7 days for each treatment group, and FIG. 4B shows a graph representing the change in pH over time.

[0056]

Table 5

[0057] As shown in Table 5, the alloy of the example had a small value of the microbial mass after 7 days and was able to suppress the growth of microorganisms. In contrast, pure magnesium was also able to suppress the growth of microorganisms. The bacteria in Example 2-3 are likely to grow in an acidic environment. However, the alloy of the example was able to suppress the growth of microorganisms equally to pure magnesium, even though the pH was lower than that of pure magnesium and the environment was more favorable for bacterial growth.

[0058] <Bacteria> (Example 2-4) Ralstonia pseudosolanacearum (MAFF 106604, Betaproteobacteria, Gram-negative bacteria) was inoculated into 3 mL of CPG medium and pre-cultured under dark conditions at 180 rpm, 30 °C for 18 hours. The main culture was carried out by inoculating 50 μL of the pre-culture solution into 1.5 mL of 1 / 3 CPG medium and culturing under dark conditions at 180 rpm, 30 °C for 24 hours. In the main culture, microorganisms were cultured in the presence of the microbial control material prepared by the process shown in FIG. 1, and the microbial control effect was evaluated. Three test groups were set up: an untreated group, a pure magnesium group, and an alloy group. No metal material was added to the untreated group, one pure magnesium sample was added to the pure magnesium group, and one plate-shaped alloy sample was added to the alloy group in the medium, respectively. The culture was carried out in triplicate. In the culture of the alloy group, the microbial control material (alloy) was added to the medium containing moisture, and the microbial control effect was evaluated while generating ions derived from the alloy material. Table 6 shows the results of measuring the turbidity of the microbial cells (hereinafter also referred to as OD600) and pH of the culture solution over time. Also, FIG. 5A shows a graph representing the change in OD600 over time for each treatment group, and FIG. 5B shows a graph representing the change in pH over time.

[0059]

Table 6

[0060] As shown in Table 6, the alloy of the example had a low OD600 after 24 hours and was able to suppress the growth of microorganisms. In contrast, for pure magnesium and the untreated case, the OD600 after 24 hours was high and the growth of microorganisms could not be suppressed. The bacteria in Examples 2-4 are likely to grow in an acidic environment. However, although the alloy of the example had a lower pH than pure magnesium and was an environment where bacteria were likely to grow, it was able to suppress the growth of microorganisms more effectively than pure magnesium.

[0061] (Example 2-5) Bacillus cereus (MAFF 118479, Bacillales, Gram-positive bacterium, spore-forming bacterium) was inoculated into 3 mL of LB medium and pre-cultured under dark conditions at 180 rpm, 30 °C for 24 hours. The main culture was carried out by inoculating 5 μL of the pre-culture solution into 1.5 mL of 1 / 3 LB medium and culturing under dark conditions at 180 rpm, 30 °C for 24 hours. At this time, one sample of each was pre-soaked 90 minutes before the start of the main culture, and then the pre-culture solution was added to start the main culture. In the main culture, microorganisms were cultured in the presence of the microbial control material prepared by the process shown in FIG. 1, and the microbial control effect was evaluated. Three test groups were set up: an untreated group, a pure magnesium group, and an alloy group. No metal material was added to the untreated group, one pure magnesium sample was added to the pure magnesium group, and one plate-shaped alloy sample was added to the alloy group, respectively, to the culture medium. The culture was carried out in triplicate. In the culture of the alloy group, the microbial control material (alloy) was added to the culture medium containing moisture, and the microbial control effect was evaluated while generating ions derived from the alloy material. Table 7 shows the results of measuring the turbidity of the bacterial cells (hereinafter also referred to as OD600) and pH of the culture solution over time. Also, FIG. 6A shows a graph representing the change in OD600 over time for each treatment group, and FIG. 6B shows a graph representing the change in pH over time.

[0062]

Table 7

[0063] As shown in Table 7, the alloy of the example had a low OD600 after 24 hours and was able to suppress the growth of microorganisms. In contrast, for pure magnesium and the untreated case, the OD600 after 24 hours was high and the growth of microorganisms could not be suppressed. The bacteria in Examples 2-5 grow easily from weakly acidic to neutral. However, although the alloy of the example was an environment where the pH was lower than that of pure magnesium and bacteria grew easily, it was able to suppress the growth of microorganisms more than pure magnesium.

[0064] (Example 2-6) Escherichia coli (MAFF 118621, Gammaproteobacteria, Gram-negative bacteria) was inoculated into 3 mL of LB medium and pre-cultured under dark conditions at 180 rpm, 30 °C for 24 hours. The main culture was carried out by inoculating 50 μL of the pre-culture solution into 1.5 mL of 1 / 3 LB medium under dark conditions at 180 rpm, 37 °C for 24 hours. In the main culture, microorganisms were cultured in the presence of the microorganism control material prepared by the process of FIG. 1, and the microorganism control effect was evaluated. Three test groups were set up: an untreated group, a pure magnesium group, and an alloy group. No metal material was added to the untreated group, one pure magnesium sample was added to the pure magnesium group, and one plate-shaped alloy sample was added to the alloy group to the medium, respectively. The culture was carried out in triplicate. In the culture of the alloy group, the microorganism control material (alloy) was added to the medium containing moisture, and the microorganism control effect was evaluated while generating ions derived from the alloy material. Table 8 shows the results of measuring the turbidity of the bacterial cells (hereinafter also referred to as OD600) and pH of the culture solution over time. Also, FIG. 7A shows a graph representing the change over time of OD600 for each treatment group, and FIG. 7B shows a graph representing the change over time of pH.

[0065]

Table 8

[0066] As shown in Table 8, the alloy of the example had a low OD600 after 24 hours and was able to suppress the growth of microorganisms. In contrast, for pure magnesium and the untreated case, the OD600 after 24 hours was high and the growth of microorganisms could not be suppressed. The bacteria in Examples 2-6 grow easily from weakly acidic to neutral. However, although the alloy of the example was an environment with a lower pH and easier for bacteria to grow than pure magnesium, it was able to suppress the growth of microorganisms more effectively than pure magnesium.

[0067] (Example 2-7) Lactobacillus fructivorans (MAFF 117341, Bacilli class, Gram-positive bacterium) was inoculated into 3 mL of MRS medium and pre-cultured under dark conditions at 180 rpm, 30 °C for 24 hours. For the main culture, 60 μL of the pre-culture solution was inoculated into 60 mL of 1 / 3 MRS medium in a 200 mL flask, and the culture was carried out under dark conditions at 180 rpm, 30 °C for 96 hours. In this main culture, microorganisms were cultured in the presence of the microorganism control material prepared by the process shown in Fig. 1, and the microorganism control effect was evaluated. Three test groups were set up: an untreated group, a pure magnesium group, and an alloy group. No metal material was added to the untreated group, one pure magnesium sample was added to the pure magnesium group, and one plate-shaped alloy sample was added to the alloy group to the medium respectively. The culture was carried out in triplicate. In the culture of the alloy group, the microorganism control material (alloy) was added to the medium containing moisture, and the microorganism control effect was evaluated while generating ions derived from the alloy material. Table 9 shows the results of measuring the turbidity of the bacterial cells (hereinafter also referred to as OD600) and pH of the culture solution over time. Also, Fig. 8A shows a graph representing the change in OD600 over time for each treatment group, and Fig. 8B shows a graph representing the change in pH over time.

[0068]

Table 9

[0069] As shown in Table 9, the alloy of the example had a low OD600 after 96 hours and was able to suppress the growth of microorganisms. In contrast, in the case of pure magnesium and untreated samples, the OD600 after 96 hours was high and the growth of microorganisms could not be suppressed. The bacteria in Examples 2-7 are acidic and easy to grow. However, the alloy of the example was able to suppress the growth of microorganisms more than pure magnesium, even though the pH of the alloy was lower than that of pure magnesium and the environment was more conducive to the growth of bacteria.

[0070] ~Growth promotion~ (Example 2-8) Fusarium sp. (MAFF 727519, Ascomycetes) was inoculated into PDA medium, and agar pieces (28.6 mm 2 , mass 0.091 g (wet weight)) that had been precultured in the dark at 25°C for 7 days were inoculated into 50 mL of PDB medium. The main culture was carried out in the dark at 25°C and 100 rpm for 7 days, and 0.5 mL of the culture solution was sampled at regular intervals to measure the pH. The cells after 7-day culture were filtered and collected using Miracloth (Merck KGaA, 475855), and the filtered cells were placed on filter paper and dried at 65°C overnight. The mass of the dried cells was measured using an electronic balance. The microspore concentration was measured using a Burker Turk hemocytometer. In the main culture, microorganisms were cultured in the presence of the microbial control material prepared by the process shown in FIG. 1, and the control effect was evaluated. As shown in Table 10, there were three test groups. No metal material was added to the untreated group, one pure magnesium sample was added to the pure magnesium group, and one plate-shaped alloy sample was added to the magnesium alloy group. The culture was carried out in triplicate. In the culture of the alloy group, microorganisms were cultured while generating ions derived from the alloy material in the presence of moisture and the microbial control material (alloy). The measurement results of the dry cell mass after 7 days, the results of measuring the pH of the culture solution over time, and the microspore concentration in the filtrate of the cells are shown in Table 10. In addition, a graph showing the comparison of the dry cell mass after 7 days for each treatment group is shown in FIG. 9A, and a graph showing the change in pH over time is shown in FIG. 9B.

[0071]

Table 10

[0072] As shown in Table 10, the alloys of the examples promoted proliferation more than the untreated control in terms of the bacterial weight after 7 days. The alloys of the examples did not promote proliferation as much as pure magnesium, but the microspore concentration in the culture filtrate after 7 days was significantly higher than that of pure magnesium. The fungus in Examples 2-8 is known as a non-pathogenic Fusarium fungus. It is known that when this fungus is mixed into the culture soil or contacted with the roots of a plant, it reduces the damage caused by highly pathogenic Fusarium fungus when the plant is infected. The alloy in the example significantly promoted the growth of spores, even though the amount of fungus recovered by Miracloth was smaller than that of pure magnesium. It is presumed that the alloy formulation changed the metabolism to prioritize growth toward spore formation over mycelium growth. Since the spores are used to inoculate plant roots, it is expected to increase the production efficiency of biological preparations.

[0073] (Examples 2-9) Rhizopus oryzae (NBRC 4707, Mucorales) was inoculated into PDA medium, and the agar pieces (28.6 mm2, mass 0.046 g (wet weight)) pre-cultured in the dark at 25°C for 5 days were inoculated into 50 mL of PDB medium. The main culture was performed in the dark at 25°C and 100 rpm for 7 days, and 0.5 mL of the culture solution was sampled over time to measure the pH. After 7 days of culture, the cells were filtered and collected using Miracloth (Merck Co., Ltd., 475855), and the filtered cells were placed on a drug packing paper and dried overnight at 65°C. The mass of the dried cells was measured using an electronic balance. In the main culture, microorganisms were cultured in the presence of the microorganism control material prepared by the process in Figure 1, and the control effect was evaluated. As shown in Table 11, there were three test areas, with no metal material added to the untreated area, one grain of pure magnesium sample added to the pure magnesium area, and one plate-shaped alloy sample added to the magnesium alloy area. Cultivation was performed in triplicate. In the alloy group, the microorganisms were cultured in the presence of water and a microorganism control material (alloy) while generating ions derived from the alloy material. Table 11 shows the measurement results of the dry cell mass after 7 days and the results of measuring the pH of the culture solution over time. Also, a graph showing the comparison of the dry cell mass after 7 days for each treatment group is shown in Fig. 10A, and a graph showing the change in pH over time is shown in Fig. 10B.

[0074]

Table 11

[0075] As shown in Table 11, for the alloy of the example, the value of the cell mass after 7 days was large, indicating that it could promote the growth of microorganisms. In contrast, for pure magnesium and the untreated case, the value of the cell mass after 7 days was small, and the growth of microorganisms could not be promoted. The bacteria in Examples 2-9 are known as beneficial filamentous bacteria that produce organic acids such as L-lactic acid, and the pH continued to decrease in the untreated group. The alloy of the example showed a lower pH than pure magnesium during the culture, suggesting that it controlled the growth promotion of the cells and regulated the metabolism to increase the production amount of organic acids such as L-lactic acid.

[0076] (Examples 2-10) Trichoderma hamatum (MAFF 236548, Ascomycetes) was inoculated into PDA medium, and an agar piece (23.7 mm 2 , mass 0.0817 g (wet weight)) that had been precultured in the dark at 25 °C for 7 days was inoculated into 50 mL of PDB medium. The culture was carried out in the dark at 25 °C and 100 rpm for 7 days, and 0.5 mL of the culture solution was sampled over time to measure the pH. The cells after 7 days of culture were filtered and collected with Miracloth (Merck KGaA, 475855), and the filtered cells were placed on filter paper and dried at 65 °C overnight. The mass of the dried cells was measured with an electronic balance. In this main culture, the microorganisms were cultured in the presence of the microbial control material prepared by the process of Fig. 1, and the control effect was evaluated. As shown in Table 12, there were 3 test groups. No metal material was added to the untreated group, 1 pure magnesium sample was added to the pure magnesium group, and 1 plate-shaped alloy sample was added to the magnesium alloy group. The culture was carried out in 3 replicates. In the cultivation of the alloy region, microorganisms were cultivated while generating ions derived from the alloy material in the presence of moisture and a microbial control material (alloy). Table 12 shows the measurement results of the dry cell mass after 7 days and the results of measuring the pH of the culture solution over time. Also, a graph representing the comparison of the dry cell mass after 7 days for each treatment group is shown in Fig. 11A, and a graph representing the temporal change in pH is shown in Fig. 11B.

[0077]

Table 12

[0078] As shown in Table 12, the alloy of the example had a large value of cell mass after 7 days and could promote the growth of microorganisms. In contrast, in the case of pure magnesium and no treatment, the value of cell mass after 7 days was small and the growth of microorganisms could not be promoted. The alloy of the example showed a lower pH than pure magnesium during cultivation, and it is presumed that the growth promotion of the cells was controlled and the metabolism was controlled to increase the production amount of acidic substances.

[0079] (Example 2-11) Trichoderma harzianum (MAFF 328304, Ascomycetes) was inoculated into PDA medium, and an agar piece (23.7 mm 2 , mass 0.1043 g (wet weight)) that had been precultured in the dark at 25°C for 7 days was inoculated into 50 mL of PDB medium. The culture was carried out in the dark at 25°C and 100 rpm for 7 days, and 0.5 mL of the culture solution was sampled over time to measure the pH. The cells after 7 days of culture were filtered and collected with Miracloth (Merck KGaA, 475855), and the filtered cells were placed on filter paper and dried at 60°C overnight. The mass of the dried cells was measured with an electronic balance. The spore formation amount (×10 4 cells / mL) was obtained by measuring the spore concentration contained in the culture filtrate with a hemocytometer. In this culture, microorganisms were cultured in the presence of the microorganism control material prepared by the process shown in Fig. 1, and the control effect was evaluated. As shown in Table 13, there were three test groups. No metal material was added to the untreated group, one pure magnesium sample was added to the pure magnesium group, and one plate-shaped alloy sample was added to the magnesium alloy group. The culture was performed in triplicate. In the culture of the alloy group, microorganisms were cultured while generating ions derived from the alloy material in the presence of moisture and the microorganism control material (alloy). Table 13 shows the measurement results of the dry cell mass after 7 days and the results of measuring the pH of the culture solution over time. Fig. 12A shows a graph representing the comparison of the dry cell mass after 7 days for each treatment group, Fig. 12B shows a graph representing the temporal change in pH, and Fig. 12C shows a graph representing the comparison of the spore formation amount (×10 4 cells / mL) after 7 days for each treatment group. In addition, Fig. 12D shows a microscopic image (100×) of the culture filtrate of the alloy group, Fig. 12E shows a microscopic image (100×) of the culture filtrate of the pure magnesium group, and Fig. 12F shows a microscopic image (100×) of the culture filtrate of the untreated group.

[0080]

Table 13

[0081] As shown in Table 13, the alloy of the example had a large value of cell mass after 7 days and could promote the growth of microorganisms. In contrast, in the case of pure magnesium and untreated samples, the value of cell mass after 7 days was small and the growth of microorganisms could not be promoted. The alloy of the example showed a lower pH than pure magnesium during the culture, and it is presumed that the growth promotion of the cells was controlled and the metabolism was controlled to increase the production amount of acidic substances.

[0082] (Example 2-12) Trichoderma virens (MAFF 425559, Ascomycetes) was inoculated on a PDA medium, and agar pieces (23.7 mm 2, a mass of 0.0623 g (wet weight) was inoculated into 50 mL of PDB medium. The main culture was carried out in the dark at 25 °C and 100 rpm for 7 days, and 0.5 mL of the culture solution was sampled over time to measure the pH. The cells after 7-day culture were filtered and collected using Miracloth (Merck KGaA, 475855), and the filtered cells were placed on filter paper and dried at 60 °C overnight. The mass of the dried cells was measured using an electronic balance. The spore formation amount (×10 4 cells / mL) was obtained by measuring the spore concentration contained in the culture filtrate using a hemocytometer. In the main culture, the microorganisms were cultured in the presence of the microbial control material prepared by the process shown in FIG. 1, and the control effect was evaluated. As shown in Table 14, there were three test groups. No metal material was added to the untreated group, one pure magnesium sample was added to the pure magnesium group, and one plate-shaped alloy sample was added to the magnesium alloy group. The culture was performed in triplicate. In the culture of the alloy group, the microorganisms were cultured while generating ions derived from the alloy material in the presence of moisture and the microbial control material (alloy). Table 14 shows the measurement results of the dry cell mass after 7 days and the results of measuring the pH of the culture solution over time. In addition, FIG. 13A shows a graph representing the comparison of the dry cell mass after 7 days for each treatment group, FIG. 13B shows a graph representing the change in pH over time, and FIG. 13C shows a graph representing the comparison of the spore formation amount (×10 4 cells / mL) after 7 days for each treatment group. In addition, FIG. 13D shows a microscopic image (100×) of the culture solution of the alloy group, FIG. 13E shows a microscopic image (100×) of the culture solution of the pure magnesium group, and FIG. 13F shows a microscopic image (100×) of the culture solution of the untreated group.

[0083]

Table 14

[0084] As shown in Table 14, the alloy of the example had a large value of cell weight after 7 days and could promote the growth of microorganisms. In contrast, in the case of pure magnesium and untreated samples, the value of cell weight after 7 days was small and the growth of microorganisms could not be promoted. The alloy of the example shows a lower pH than pure magnesium during cultivation, and it is presumed that it controls the growth promotion of the bacterial cells and controls the metabolism to increase the production amount of acidic substances.

[0085] In this specification, the PDA medium is an agar medium composed of 39 g / L BD Difco potato dextrose agar (Nippon Becton Dickinson Co., Ltd., 213400) and 3 g / L agar (FUJIFILM Wako Pure Chemical Corporation, 010-15815). In this specification, the LB medium is a liquid medium composed of 25 g / L LB medium, Miller (Nacalai Tesque, Inc., 20068-75). In this specification, the 1 / 3LB medium is a liquid medium composed of the LB medium and twice the amount of sterilized water. In this specification, the YSA medium is an agar medium composed of 4 g / L Bacto yeast extract (Nippon Becton Dickinson Co., Ltd., 212750), 2 g / L Bacto malt extract (Nippon Becton Dickinson Co., Ltd., 218630), 15 g / L soluble starch (Nacalai Tesque, Inc., 32122-75), 0.5 g / L dipotassium hydrogen phosphate (Nacalai Tesque, Inc., 28726-05), 0.5 g / L magnesium sulfate heptahydrate (FUJIFILM Wako Pure Chemical Corporation, 131-15275), and 18 g / L agar (FUJIFILM Wako Pure Chemical Corporation, 010-15815). In this specification, the CPG medium is a liquid medium composed of 10 g / L polypeptone (Nippon Pharmaceutical Co., Ltd., 394-00115), 1 g / L casamino acids (Daiichi) (Nippon Pharmaceutical Co., Ltd., 392-00655), and 5 g / L glucose (FUJIFILM Wako Pure Chemical Corporation, 049-31165). In this specification, the 1 / 3CPG medium is a liquid medium composed of the CPG medium and twice the amount of sterilized water. In this specification, the PDB medium is a liquid medium composed of 24 g / L BD Difco potato dextrose broth (Nippon Becton Dickinson Co., Ltd., 254920). In this specification, the MRS medium is a liquid medium composed of 55 g / L BD Difco Lactobacillus MRS Broth (Becton, Dickinson and Company Japan, 288130). In this specification, the 1 / 3 MRS medium is a liquid medium composed of the MRS medium and twice the amount of sterilized water.

Claims

**Claim 1** A microbial control material containing an alloy material, wherein the alloy material contains magnesium as a main component. **Claim 2** The microbial control material according to claim 1, wherein the alloy material further contains at least one element selected from the group consisting of zinc, calcium, boron, silicon, phosphorus, sulfur, potassium, manganese, iron, nickel, copper, molybdenum, and silver. **Claim 3** The microbial control material according to claim 2, wherein the total content of the elements is 0.1% by mass to 20% by mass based on the total mass of the alloy material. **Claim 4** The microbial control material according to claim 1 or claim 2, wherein the alloy material further contains aluminum. **Claim 5** A microbial control composition containing ions derived from an alloy material and a liquid, wherein the alloy material contains magnesium as a main component. **Claim 6** The microbial control composition according to claim 5, wherein the alloy material further contains at least one element selected from the group consisting of zinc, calcium, boron, silicon, phosphorus, sulfur, potassium, manganese, iron, nickel, copper, molybdenum, and silver. **Claim 7** A method for using a microbial control material, comprising an ion generation step of generating ions derived from the alloy material by bringing a microbial control material containing an alloy material, wherein the alloy material contains magnesium as a main component, into contact with a liquid and leaving it standing.

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