Antimicrobial agent, bacteriostatic agent, and wound dressing using the same

Abstract

To provide an antimicrobial agent and a bacteriostatic agent, wherein Thujopsis dolabrata oil and Thujopsis dolabrata water extracted from natural Cupressaceae species, a water-soluble alkali, and funoran are dispersed or dissolved in water, and spray-dried to form fine granules, and to provide an antimicrobial fiber material wherein the antimicrobial agent and bacteriostatic agent are dissolved in water, impregnated, and dried, and to provide a wound dressing prepared using the antimicrobial agent and bacteriostatic agent, wherein the antimicrobial agent is mixed into a resin melt obtained by heat-melting and then molded.SOLUTION: As a bacteriostatic agent of a wound dressing, a purely plant-derived wood such as dolabrata, cypress, is used as a raw material, the raw material being made into chip material, the chip material being steamed in a high-temperature steaming kettle, and, when cooled, yielding sap (oil and water). The extracted oil contains 85% or more of thujopsene, 1% or more of hinokitiol, and 1% or more of β-dolabrin. By utilizing these components, the oil is diluted to 0.5% or more, sprayed, and adhered to gauze or pulp material to enhance antibacterial properties. The extracted oil and water are processed into gauze or pulp material and used for treatment of wound sites. Recovery of the wound site can be expected due to the bacteriostatic action.SELECTED DRAWING: None

Description

Technical Field

[0001] The present invention relates to an antibacterial agent, an antibacterial agent that suppresses the generation and growth of mold and bacteria and does not pollute the environment, and a wound dressing material using these agents.

[0002] Conventional antibacterial agents are poorly water-soluble, so it is basically impossible to adhere (fix) them to hydrophilic fiber materials such as natural fibers. Even if the immersion treatment is carried out for a long time, the antibacterial agent is not sufficiently fixed. Therefore, when the fiber material is washed with water, the antibacterial agent is easily washed away. Therefore, the antibacterial property of the fiber material was hardly maintained or continued.

[0003] In addition, volatile or sublimable fungicides are also known, but their effects are low unless they are in a sufficiently airtight space. Furthermore, the antibacterial agents used are required to be non-toxic to humans or plants and not to have an adverse effect on purification bacteria in wastewater. There has been a demand for an antibacterial agent that has both these properties and the above-mentioned adhesiveness.

Summary of the Invention

[0004] An object of the present invention is to provide an antibacterial agent and an antibacterial agent characterized in that hinoki oil and hinoki water extracted from the natural cypress family are dispersed or dissolved in water together with a water-soluble alkali and furan, and spray-dried to form fine particles.

[0005] Another object of the present invention is to provide an antibacterial fiber material characterized in that the above antibacterial agent and antibacterial agent are dissolved in water, impregnated and dried, and a wound dressing material using the above antibacterial agent, which is characterized in that it is mixed with a resin melt obtained by thermally melting the antibacterial agent and molded. The cause of the wound is said to be Pseudomonas aeruginosa. Means for Solving the Problems

[0006] In order to solve the above problems, the present invention comprises the following means 1) to 2). That is, 1) An antibacterial agent and a bacteriostatic agent, characterized in that natural ate wood is dispersed or dissolved in water with a solution extracted from the genus Chamaecyparis, a water-soluble alkali, and furan, and spray-dried to form fine particles. 2) A wound dressing material obtained by dissolving the antibacterial agent and the bacteriostatic agent described in 1) in water. Effects of the Invention

[0007] According to the present invention, a wound healing effect can also be obtained.

Brief Description of the Drawings

[0008] [Figure 1] These are the results of the Kaken Test Center, a general incorporated association. [Figure 2] These are the results of the Kaken Test Center, a general incorporated association.

Embodiments for Carrying Out the Invention

[0009] Hereinafter, preferred embodiments for obtaining the antibacterial agent, the bacteriostatic agent, and the wound dressing material using them according to the present invention will be described.

[0010] Ate is the local name of Chamaecyparis obtusa var. chamaecyparis, which is a variety of Chamaecyparis obtusa. It is the prefectural tree of Ishikawa Prefecture and is widely planted on the Noto Peninsula. Trees grown under the name of ate (ma-ate, kusa-ate, kana-ate, eso-ate, suzu-ate) in Ishikawa Prefecture and those grown under the name of hiba in Aomori Prefecture are both considered to be Chamaecyparis obtusa in plant taxonomy.

[0011] The hiba oil and hiba aqueous solution used in the present invention are obtained by steam-distilling chips or sawdust from the trunk of hiba (Chamaecyparis) and are commercially available separately. These hiba oil and hiba aqueous solution contain water-soluble natural tropolones such as hinokitiol and β-drabren, which have antibacterial properties.

[0012] The hinoki oil, hinoki aqueous solution used in the present invention, or a water-soluble resin such as methylcellulose, carboxymethylcellulose, polyvinyl alcohol, etc. are dissolved in water. The solution temperature at that time varies depending on the symptoms of the water-soluble resin used, etc., but it may be appropriately set within the range of -10 to 120°C. Incidentally, the hinoki oil and hinoki aqueous solution are added at an addition ratio of 0.01 to 30% with respect to the water-soluble resin. If it is less than 0.01%, the antibacterial effect is not sufficient, and if it is more than 30%, not only will floating occur, but there will be no improvement in the antibacterial and bacteriostatic effects, so it becomes uneconomical.

[0013] The above solution is made into fine granular powder by a known spray drying method. This drying temperature may also be appropriately set within the range of -20°C to 160°C. For example, it may be dried by natural wind power. Incidentally, when using hinoki oil, the hinoki oil will not completely dissolve in water and a part of the oil component will float on the liquid surface. Even in this case, if it is spray-dried while stirring sufficiently, a fine granular antibacterial agent and bacteriostatic agent can be produced.

[0014] The antibacterial agent and bacteriostatic agent thus produced are water-soluble and moreover natural agents, so they are harmless to the human body and plants.

[0015] In order to fix the above antibacterial agent and bacteriostatic agent to the fiber material, the above antibacterial agent and bacteriostatic agent are dissolved in water at a ratio of 0.01 to 30%. This solution is applied to the fiber material, or the fiber material is immersed in the above solution for 2 to 30 minutes and dried appropriately within the range of -20 to 160°C. For example, it may be dried by natural wind power. This fiber material may be filamentous cellulose or a woven fiber product. As the material, protein fibers such as silk and wool are preferable, but acrylic fibers etc. can be applied.

[0016] The fabric material thus produced possesses antibacterial and bacteriostatic properties, and even after dozens of washes, most of the antibacterial and bacteriostatic agents are not washed away but remain fixed and remain in the fabric material, maintaining the antibacterial and bacteriostatic effects for a long period of time. Although the detailed reasons for this are unknown, it is presumed that the antibacterial and bacteriostatic agents penetrate deep into the fibers because they are water-soluble, and that the water-soluble resin added together with the antibacterial and bacteriostatic agents is fixed to the fabric material and denatured by heat or other factors, becoming insoluble in water.

[0017] Furthermore, when antibacterial and bacteriostatic agents are dissolved in the dye solution during the dyeing process, the antibacterial and bacteriostatic agents can be fixed during the dyeing process, which essentially eliminates additional processes and makes the material extremely valuable for practical industrial production.

[0018] On the other hand, in order to fix the antibacterial agent or bacteriostatic agent of the present invention to a resin molded product, the powdered antibacterial agent or bacteriostatic agent may be added directly to a heat-melted resin melt at a ratio of 0.01 to 20%, and molded into an appropriate shape at an appropriate molding temperature in the range of 20 to 160° C. This resin molded product may be molded into a long thread shape, a plate shape, or any other shape, and polyvinyl chloride, polypropylene, polyethylene, nylon, etc. may be used as the material.

[0019] The resin molded article thus produced has antibacterial and bacteriostatic properties, and may be molded into long filaments and then woven into fibers. If an antibacterial or bacteriostatic agent is added together with an additive such as a plasticizer during molding, the antibacterial or bacteriostatic agent can be fixed to the resin molded article while it is being plasticized (softened), which essentially eliminates additional processes and is of great practical value in industrial production.

[0020] As explained above, the antibacterial and bacteriostatic agents of the present invention are made from hiba oil and hiba water extracted from natural thuja trees, and therefore can inhibit or eliminate the growth and proliferation of mold and fungi without polluting the environment.

[0021] Furthermore, since the antibacterial and bacteriostatic agents are water-soluble, they have high permeability when fixed to fibrous materials, and the antibacterial and bacteriostatic agents reach and adhere to the interior of the fibrous material. Furthermore, since they contain a water-soluble resin, they can be maintained even after being sufficiently dried.

[0022] Furthermore, when producing the above-mentioned fiber material having antibacterial and bacteriostatic properties, it is only necessary to add an antibacterial or bacteriostatic agent to a dye solution and then perform a dipping treatment. When producing a resin molded product having antibacterial and bacteriostatic properties, it is only necessary to add an antibacterial or bacteriostatic agent when adding a plasticizer. Therefore, there is essentially no need to add a step to the conventional processing steps, and this method is extremely valuable in terms of practical factory production.

[0023] In this antibacterial and bacteriostatic test, the results of the JIS L1902:2015, 8.1 bacterial liquid absorption method showed that the antibacterial activity value for Staphylococcus aureus was 5.7 for the original product and 5.4 after 50 washes. The antibacterial activity value for Klebsiella pneumoniae was 6.2 for the original product and 5.7 after 50 washes. The antibacterial activity value for MRSA was 5.8 for the original product and 5.8 after 50 washes. Antibacterial or bacteriostatic activity values of 2.2 or higher for Staphylococcus aureus, Klebsiella pneumoniae, and MRSA are considered to be antibacterial or bacteriostatic. It is concluded that an antibacterial activity value of 2.2 or higher (including 2.2) is appropriate as the evaluation standard for antibacterial and deodorizing effects. Based on the test results, the antibacterial activity values of the antibacterial and bacteriostatic agent of this invention after 50 washes were 5.4 for Staphylococcus aureus, 5.7 for Klebsiella pneumoniae, and 5.8 for MRSA, demonstrating sufficient antibacterial and bacteriostatic effects. Antibacterial effect: Certification standard for antibacterial testing of SEK mark antibacterial processing Antibacterial activity value > Growth value F Kaken Test Center General Incorporated Association Test Number No. OS-20-003895 (Figure 1)

[0024] In this Pseudomonas aeruginosa test, the results of the JIS L1902:2015, 8.1 bacterial liquid absorption method showed that the antibacterial activity value was 6.2 for pulp material and 6.3 for gauze, with a growth value F of 2.4.It is said that if the antibacterial activity value is higher than the growth value F, the material is effective against Pseudomonas aeruginosa. It can prevent the growth of Pseudomonas aeruginosa, which is said to be the cause of suppuration associated with bedsores (pressure ulcers). Antibacterial effect: Certification criteria for antibacterial property tests of SEK mark bacteriostatic processing Antibacterial activity value > Growth value F Japan Kaken Testing Center, Incorporated Association Test numbers No.OS-23-051938 No.OS-23-028459 (Figure 2)

[0025] The present invention provides an antibacterial agent and a bacteriostatic agent, which are characterized in that hinoki oil and hinoki water extracted from natural Chamaecyparis obtusa are dispersed or dissolved in water together with a water-soluble alkali and furan, and then spray-dried to form fine granules. The present invention aims to provide an antibacterial fiber material characterized in that the above antibacterial agent and bacteriostatic agent are dissolved in water, impregnated and dried, and a wound dressing material using the antibacterial agent and bacteriostatic agent, which is characterized in that the above antibacterial agent is mixed into a resin melt obtained by heat-melting and then molded.

[0026] As a bacteriostatic agent for wound dressings, pure plants are applied, using wood such as Chamaecyparis as raw materials. The raw materials are made into chip materials, which are steamed in a high-temperature steamer and then cooled to obtain sap (oil and moisture). The extracted oil components contain more than 85% of thujopsene, more than 1% of hinokitiol, and more than 1% of β-drimene. Using these components, it is diluted by 0.5% or more and sprayed to adhere to gauze and pulp materials to enhance bacteriostatic properties. The extracted oil and moisture are processed into gauze and pulp materials for use in the treatment of wound parts. The recovery of the wound part can be achieved by the bacteriostatic effect.

Claims

1. An antibacterial and bacteriostatic agent, characterized in that natural ates, a solution extracted from the Chamaecyparis obtusa family, a water-soluble alkali, and furan are dispersed or dissolved in water and spray-dried to form fine granulars.

2. A wound dressing material obtained by dissolving the antibacterial and bacteriostatic agent according to Claim 1 in water and using it.

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