Cosmetic method applying iontophoresis and ultrasound

Abstract

To enhance the effect of reducing the visibility of pores through active ingredients for skin.SOLUTION: The present invention provides a cosmetic method which includes applying a skin-active ingredient effective for ameliorating parakeratosis, followed by application of iontophoresis together with additional application of ultrasound. This can enhance the effect of reducing the visibility of pores.SELECTED DRAWING: Figure 1

Description

[Technical Field]

[0001] The present invention relates to a cosmetic method for applying iontophoresis and ultrasound, and a cosmetic composition for use when applying iontophoresis and ultrasound. [Background technology]

[0002] Topical skin preparations contain a variety of active ingredients, including antioxidants, circulation promoters, whitening agents, moisturizers, and vitamins. However, water-soluble drugs are difficult to absorb percutaneously due to the formation of tight junctions between cells in the stratum corneum, particularly the granular layer. To enhance percutaneous absorption, various methods have been developed, including surfactants, nanoparticles, liposomes, electroporation, and microneedles. In recent years, iontophoresis (ion introduction) technology has been used to promote percutaneous or mucosal absorption of water-soluble drugs in medical fields such as dentistry, ophthalmology, and urology, as well as in the cosmetic field.

[0003] Iontophoresis involves applying a relatively low current (e.g., 10 V, 0.5 mA / cm) to the skin. 2 This technique promotes transdermal delivery of water-soluble drugs and peptide substances, which inherently have low skin permeability, by applying a voltage (approximately 100 V) for several seconds to several hours. For example, iontophoresis has been reported for water-soluble steroids (Patent Document 1) and lidocaine salts as local anesthetics (Patent Document 2). In the cosmetic field, a method has been reported in which negatively charged active ingredients, such as vitamin C, glycylglycine, and tranexamic acid, are applied and then iontophoresis from the negative electrode is performed to enhance their transdermal delivery (Patent Documents 3 and 4). A method of transdermal delivery using iontophoresis from the positive electrode is also known (Patent Document 5). Glycylglycine, which has a pore-constricting effect, is particularly known as a component that is difficult to deliver transdermally due to its water solubility. Based on its electrical property of allowing current to easily flow through appendages such as pores, iontophoresis from the negative electrode is performed with the aim of accumulating glycylglycine in areas surrounding pores (Patent Document 3). [Prior art documents] [Patent documents]

[0004] [Patent Document 1] International Publication No. 96 / 011034 Brochure [Patent Document 2] Japanese Patent Application Publication No. 10-316590 [Patent Document 3] Japanese Patent Application Laid-Open No. 2010-260796 [Patent Document 4] Japanese Patent Application Laid-Open No. 2006-298850 [Patent Document 5] Japanese Patent Application Laid-Open No. 2007-130076 Summary of the Invention [Problem to be solved by the invention]

[0005] There is a demand for improved penetration of active ingredients into the skin. [Means for solving the problem]

[0006] In order to solve the above problems, the present inventors have conducted extensive research and have surprisingly found that the effect of reducing the visibility of pores can be enhanced by applying ultrasound when applying iontophoresis after applying glycylglycine, which led to the present invention. The present invention therefore relates to: [1] A step of applying a composition containing a skin active ingredient having a molecular weight of 100 to 1000 Da to the skin; and applying iontophoresis and ultrasound simultaneously from the negative electrode side to the skin after application. [2] The cosmetic method according to item 1, wherein the active ingredient for skin is an amino acid derivative, or glycyrrhizinic acid or a salt thereof, or a derivative thereof. [3] The cosmetic method according to item 1, wherein the amino acid derivative is a glycine derivative or a salt thereof. [4] The cosmetic method according to Item 3, wherein the glycine derivative is glycylglycine. [5] The cosmetic method according to item 1, wherein the cosmetic method promotes penetration of the active ingredient for skin into the stratum corneum. [6] The beauty method described in item 1 for pore care. [7] The cosmetic method according to item 1, wherein the composition further contains 2.0 to 50% polyhydric alcohol as a moisturizer. [8] The cosmetic method according to Item 7, wherein the polyhydric alcohol is a dihydric or trihydric alcohol. [9] The method according to Item 6, wherein the polyhydric alcohol is 1,3 butylene glycol or dipropylene glycol.

[10] The cosmetic method according to item 1, wherein the composition further contains 0.1 to 10% of a block copolymer comprising a lipophilic portion and a hydrophilic portion as a penetration enhancer.

[11] The cosmetic method according to item 1, wherein the output of the iontophotorhesis is 0.1 mA to 2.5 mA, and the frequency of the ultrasound is 0.5 MHz to 10 MHz.

[12] Ultrasonic power is 0.1W / cm 2 ~1.0W / cm 2 Item 2. The cosmetic method according to Item 1,

[13] A cosmetic composition for use in conjunction with iontophoresis and ultrasound application, comprising an active ingredient for skin having a molecular weight of 100 to 1000 Da.

[14] The cosmetic composition according to Item 13, wherein the active ingredient for skin is an amino acid derivative, or glycyrrhizinic acid or a salt thereof, or a derivative of glycyrrhizinic acid.

[15] The cosmetic composition according to Item 13, wherein the active ingredient for skin is a glycine derivative or a salt thereof.

[16] The cosmetic composition according to Item 13, further comprising at least one component selected from the group consisting of a penetration enhancer, a moisturizer, and an oil.

[17] 0.01–10% glycylglycine 0.1-10% penetration enhancer 2.0-50% moisturizer Item 14. The cosmetic composition according to item 13, comprising: [Effects of the Invention]

[0007] This promotes penetration of active ingredients into the skin. [Brief explanation of the drawings]

[0008] [Figure 1] FIG. 1 is a graph showing the amount of glycylglycine that penetrates the stratum corneum when iontophoresis and ultrasound are applied after application of glycylglycine. DETAILED DESCRIPTION OF THE INVENTION

[0009] The present invention relates to a method for treating a skin condition, the method comprising the steps of: applying a composition containing a skin active ingredient to the skin; applying iontophoresis and ultrasound to the skin after application; The present invention relates to a cosmetic method comprising: a) enhancing the penetration of an active ingredient for skin into the stratum corneum, thereby increasing the efficacy of the active ingredient for skin on skin cells, particularly epidermal cells and / or dermal cells; b) treating, preventing, or improving parakeratosis, particularly for the purpose of caring for pores; c) achieving an improving effect on pores, i.e., improving the enlargement, prominence, clogging, and / or darkening of pores, and shrinking pores; d) providing a cosmetic method that does not fall under the category of an action performed by a physician, and can be considered a so-called non-therapeutic method.

[0010] The cone-shaped depression around the pore is recognized as a pore, and when this area widens, it is recognized as an open pore. When pores are prominent, the stratum corneum in the cone-shaped depression around the pore is often in a parakeratotic state. Parakeratosis refers to improper stratum corneum differentiation, a state in which nuclei that normally disappear during stratum corneum differentiation remain. Skin exhibiting parakeratosis has poor skin barrier function and moisturizing ability, leading to dry and rough skin and enlarged pores. One cause of parakeratosis is increased production of unsaturated fatty acids in sebum. While not intending to be limited by theory, it is thought that oxidation of unsaturated fatty acids accumulated in pores results in oxides that act on epidermal cells, disrupting stratum corneum turnover, causing parakeratosis, and enlarged pores. Penetration of active skin ingredients into the parakeratotic stratum corneum improves parakeratosis and reduces pore enlargement, thereby exerting a pore contraction effect.

[0011] The active ingredient for skin refers to any low-molecular-weight compound having a molecular weight of 100 Da to 1000 Da, more preferably 100 Da to 500 Da, and having a beneficial physiological effect on the skin. From the viewpoint of applying iontophoresis, the active ingredient for skin may be a water-soluble low-molecular-weight compound. Such ingredients are well known in the cosmetic field and can be incorporated into cosmetics. Examples of such active ingredients for skin include amino acid derivatives or glycyrrhizinic acid, salts thereof, or derivatives thereof.

[0012] The term "amino acid derivative" refers to any compound derived from an amino acid via its amino group, carboxyl group, or side chain group, and includes peptides. Among peptides, dipeptides and tripeptides are particularly preferred, and from the viewpoint of physiological effects on the skin, glycine derivatives, particularly glycylglycine, or a salt thereof, or a derivative thereof, are more preferred. Examples of glycine derivatives include the following: [ka] (In the formula, R1 and R 2 each independently represents a hydrogen atom, an alkyl group, an alkenyl group, an aryl group, an aralkyl group, an aminomethylcarbonyl group, an amidino group, an alkylcarbonyl group, an alkenylcarbonyl group, an arylcarbonyl group, or an aralkylcarbonyl group, R 3 is a hydrogen atom, an alkyl group, an alkenyl group, an aryl group, or an aralkyl group. Examples of the compound include compounds represented by the following formula: In particular, R 1 , R 2 Preferably, one of R is a hydrogen atom and the other is a methyl group, an aminomethylcarbonyl group, or a benzylcarbonyl group. 3 is preferably a hydrogen atom or an alkyl group, and the alkyl group is preferably a methyl group, an ethyl group, an n-butyl group, or a t-butyl group. 3 is particularly preferably a hydrogen atom or an ethyl group. 1 , R 2 and R 3 cannot be hydrogen atoms at the same time. 1 and R 2 is a hydrogen atom, and particularly, an alkyl ester having 1 to 4 carbon atoms or a benzyl ester is preferred.

[0013] Specific examples of glycine derivatives and salts thereof include sarcosine (N-methylglycine), N-ethylglycine, N-propylglycine, N-diethylglycine, N-dimethylglycine, N-amidinoglycine, N-amidino-N-methylglycine, glycylglycine, phenaceturic acid, glycine methyl ester hydrochloride, glycine ethyl ester hydrochloride, glycine n-butyl ester hydrochloride, glycine t-butyl ester hydrochloride, glycine n-propyl ester hydrochloride, glycine n-pentyl ester hydrochloride, and glycine benzyl ester hydrochloride.

[0014] Among these, sarcosine, glycylglycine, phenaceturic acid, glycine ethyl ester hydrochloride, and glycine benzyl ester hydrochloride are preferred, with sarcosine, glycylglycine, and phenaceturic acid being particularly preferred. Glycylglycine is particularly preferred because it has an effect of improving enlarged pores. Glycylglycine is represented by the following formula: [ka] Glycylglycine is an amphoteric compound with a carboxyl group and an amino group, and its dissociation state varies depending on the pH. Its isoelectric point is approximately 5.65, pKa1 is 3.12, and pKa2 is 8.17. When the pH is lowered below the isoelectric point, glycylglycine becomes positively charged, while when the pH is raised above the isoelectric point, it becomes negatively charged.

[0015] The dissociated state of glycylglycine is shown below: [ka]

[0016] The composition of the present invention may contain salts. - Since anions such as these become competing ions for glycylglycine, the amount of salts such as NaCl is preferably 0.5% by mass or less, more preferably 0.1% by mass or less, and even more preferably substantially free of salts.

[0017] Glycyrrhizic acid has the following formula: [ka] It is a compound represented by the formula: Glycyrrhizic acid may form an inorganic salt or an organic salt. Inorganic salts may include sodium salt, potassium salt, calcium salt, magnesium salt, and ammonium salt, with dipotassium glycyrrhizinate being particularly preferred. Organic salts may include pyridine salt and triethylamine salt. Derivatives may be modified with any group, and the hydroxyl and carboxyl groups contained therein may form esters, peptides, or ethers. Glycyrrhizic acid is an acid having multiple carboxyl groups, and therefore dissociates and becomes negatively charged in weakly acidic to alkaline conditions, i.e., pH 5.5 or higher.

[0018] The composition containing the active ingredient for skin may be a cosmetic or a pharmaceutical. The concentration of the active ingredient for skin can be determined appropriately depending on the type and intended efficacy of each active ingredient for skin. The upper limit of the concentration of the active ingredient for skin can be selected from the viewpoint of the stability of the cosmetic, and 10%, 7%, 5%, or 2% can be selected. The lower limit of the active ingredient for skin can be selected from the viewpoint of the effect of reducing the visibility of pores, and 0.01%, 0.1%, or 0.5% can be selected. The upper and lower limits of the active ingredient for skin concentration can be combined in any way to form a range.

[0019] The composition of the present invention is preferably a cosmetic composition for use in conjunction with iontophoresis and ultrasonic treatment. Therefore, the composition of the present invention can be formulated as a topical skin preparation. The topical skin preparation is not particularly limited as long as it is applicable to the skin, and any formulation can be used, such as a solution, emulsion, solid, semi-solid, powder, powder dispersion, water-oil two-layer separation, water-oil-powder three-layer separation, ointment, gel, aerosol, mousse, or stick. It can be provided in the form of an aqueous solution, a gel-like aqueous solution, a water-containing molded gel, a gel-like ointment, a sheet preparation carrying a water-containing molded gel, or an O / W emulsion containing an oil such as squalane or ester oil, suitable for iontophoresis and ultrasonic treatment. For example, an aqueous solution, a gel-like preparation, or an O / W emulsion can be applied directly to the skin, or a woven or nonwoven fabric such as gauze can be impregnated with the composition and applied to the skin. Alternatively, a sheet-like composition can be applied directly to the skin. Alternatively, the composition may be contained in a drug reservoir integrated into the electrode structure of the iontophoresis device and brought into contact with the skin via a pad, an ion exchange membrane, etc. The application site is not particularly limited, and may be any skin on the body, such as the face, arms, back, legs, or chest.

[0020] In addition to the active ingredient for skin, the composition of the present invention may contain penetration enhancers, moisturizers, thickeners, pH adjusters, bases, stabilizers, other active ingredients, excipients, emulsifiers, fragrances, etc. that are commonly used in topical skin preparations.

[0021] Examples of penetration enhancers include block copolymers containing a lipophilic portion and a hydrophilic portion. Examples of such block copolymers include polyoxyethylene (POE) 14 / polyoxypropylene (POP) 7 dimethyl ether. The incorporation of polyoxyethylene (POE) / polyoxypropylene (POP) dimethyl ether promotes the penetration of active ingredients for skin through iontophoresis and ultrasonic treatment. The penetration enhancer can be incorporated at 0.1 to 10%, and is preferably incorporated at 1% or more from the viewpoint of enhancing electrical conductivity and / or penetration.

[0022] Examples of moisturizing agents include, but are not limited to, polyhydric alcohols, sorbitol, fructose, mannose, erythritol, trehalose, xylitol, chondroitin sulfate, hyaluronic acid, mucoitin sulfate, and caronic acid. Among these moisturizing agents, polyhydric alcohols are preferred from the viewpoint of suppressing precipitation of active ingredients for skin. Examples of polyhydric alcohols include dihydric and trihydric alcohols. More specific examples include ethylene glycol, propylene glycol, butylene glycol, glycerol, and diglycerol. More specific examples include 1,3-butylene glycol and dipropylene glycol. The inclusion of polyhydric alcohols not only provides moisturizing effects but also enhances the effect of glycylglycine in improving enlarged pores. The moisturizer may be blended in at 2.0 to 50%. From the viewpoint of a comfortable feel on the skin when performing iontophoresis, it is preferable to blend it in at, for example, 5% or more, 10% or more, or 15% or more, and from the viewpoint of electrical conductivity, it is even more preferable to blend it in at 30% or less, 25% or less, or 20% or less.

[0023] Thickening agents include, but are not intended to be limited to, gum arabic, carrageenan, karaya gum, tragacanth gum, carob gum, quince seed (marmelo), casein, dextrin, gelatin, sodium pectinate, sodium alginate, methylcellulose, ethylcellulose, carboxymethylcellulose (CMC), hydroxyethylcellulose, hydroxypropylcellulose, polyvinyl alcohol (PVA), polyvinyl methyl ether (PVM), polyvinylpyrrolidone (PVP), sodium polyacrylate, carboxyvinyl polymers, locust bean gum, guar gum, tamarind gum, dialkyldimethylammonium cellulose sulfate, xanthan gum, agar, bentonite, hectorite, AlMg silicate (veegum), laponite, and the like. The viscosity of the composition is low, for example, liquid to gel-like, specifically 100 mPa·s to 100,000 mPa·s, preferably 1,000 mPa·s to 10,000 mPa·s, and more preferably 1,000 mPa·s to 5,000 mPa·s. The amount of thickener to be added is determined appropriately so as to achieve the viscosity of the composition described above, and is, for example, 0.1% to 5.0%.

[0024] Examples of pH adjusters that can be used include, but are not limited to, phosphoric acid or a salt thereof, malic acid or a salt thereof, citric acid or a salt thereof, lactic acid or a salt thereof, TRIS, and HEPES.

[0025] The base may be water or an organic solvent, and examples of the organic solvent include alcohols such as ethanol and isopropanol, but are not limited to these.

[0026] Stabilizers include, but are not intended to be limited to, preservatives such as phenoxyethanol and parabens, and antioxidants such as butylhydroxytoluene, tocopherol, and phytin.

[0027] Other active ingredients include, but are not intended to be limited to, vitamins and derivatives thereof, such as retinol or retinol derivatives (vitamin A), nicotinamide or derivatives thereof (vitamin B), ascorbic acid or derivatives thereof (vitamin C), tocopherol or derivatives thereof (vitamin E), plant extracts, seaweed extracts, arbutin, hydroquinone, and the like.

[0028] In the present invention, transdermal absorption can be significantly enhanced by negatively charging the active ingredient for skin and subjecting it to iontophoresis from a negative electrode. The composition of the present invention preferably uses a pH that allows the active ingredient for skin to be negatively charged. In the case of glycylglycine, a pH higher than 5.65 is sufficient, and in the case of glycyrrhizic acid, a pH higher than 5.0 is sufficient. From the viewpoints of promoting the delivery of the active ingredient for skin by iontophoresis, as well as stability and safety, the composition of the present invention preferably has a pH of 5.5 to 8.5, more preferably 6.0 to 8.0. Active ingredients for skin, particularly glycylglycine, become unstable at a pH higher than 8.5, and there is concern that they may damage the stratum corneum. Furthermore, a pH lower than 6.5 may result in insufficient transdermal absorption promotion. From the viewpoints of promoting the delivery of the active ingredient for skin by iontophoresis, as well as stability and safety, the composition of the present invention preferably has a pH of 5.5 to 8.5, more preferably 6.0 to 8.0.

[0029] For example, the pH of the composition of the present invention can be adjusted to the desired pH as described above by blending any basic substance alone or in combination of two or more thereof, such as organic amines such as KOH, NaOH, triethanolamine, 2-amino-2-methyl-1,3-propanediol, and 2-amino-2-methyl-1-propanol, and basic amino acids such as arginine. The amount of these basic substances to be blended is an amount that can achieve the desired pH, and varies depending on the type of basic substance used, the amount of the active ingredient for skin, and the like, and is not limited.

[0030] All documents mentioned in this specification are incorporated herein by reference in their entirety. The examples of the present invention described below are for illustrative purposes only and do not limit the technical scope of the present invention. The technical scope of the present invention is limited only by the claims. Modifications of the present invention, such as additions, deletions, and substitutions of constituent elements of the present invention, can be made without departing from the spirit of the present invention.

[0031] The skin to which the composition containing the active ingredient for skin is applied may be any part of the skin, but from the viewpoint of exerting the effect of reducing the visibility of pores, it is preferable to apply the composition to parts of the skin where pores are noticeable, such as the face, limbs, abdomen, etc. Among the parts of the face, it is preferable to apply the composition to parts such as the nose, cheeks, and forehead. Sebaceous glands are connected to the pores, and when a large amount of sebum is produced, the pores become clogged and become noticeable.

[0032] [Iontophoresis] In the cosmetic method of the present invention, any method of iontophoresis may be used as long as it can deliver a skin active ingredient transdermally. More specifically, iontophoresis is performed by applying a voltage to the skin after application of the composition by placing a probe electrically connected to a positive or negative electrode. Any commonly used iontophoresis method, device, voltage application conditions, probe type, etc. may be used. When applying iontophoresis, the negative or positive electrode is selected depending on the charge of the skin active ingredient. When a negatively charged skin active ingredient is applied, iontophoresis is performed from the negative electrode, whereas when a positively charged skin active ingredient is applied, iontophoresis is performed from the positive electrode, thereby allowing the skin active ingredient to penetrate deep into the stratum corneum. For example, when glycylglycine, glycyrrhizic acid or a salt thereof, or a derivative thereof is used as the skin active ingredient, these skin active ingredients are negatively charged in weakly acidic to alkaline, particularly neutral to alkaline, conditions. Therefore, voltage is applied from the negative electrode side so that the electrode applied to the skin becomes the negative electrode. Platinum, carbon, silver, silver chloride electrodes, etc. can be used as the electrode, and any method of current application, such as direct, pulse, or pulse depolarization, can be used.

[0033] The current density is not limited, but is preferably 0.001 to 0.5 mA / cm 2 and more preferably 0.01 to 0.4 mA / cm 2 and more preferably 0.05 to 0.3 mA / cm 2 0.001mA / cm 2 If the concentration is less than 0.5 mA / cm, the transdermal delivery of glycylglycine may be insufficient. 2 If the probe area exceeds 7cm, skin irritation may occur. The probe and voltage can be selected to achieve such a current density. Therefore, the larger the probe area, the higher the voltage must be applied to achieve a given current density. For example, 2When this probe is used, the voltage can be selected from 0.035 to 1.75V.

[0034] The treatment time for iontophoresis is not particularly limited, but is usually 30 seconds to 30 minutes, and more preferably 1 to 20 minutes. The number of treatments and intervals between treatments may also be arbitrary.

[0035] [Ultrasonic treatment] In the cosmetic method of the present invention, ultrasonic treatment is applied by configuring a probe that comes into contact with the skin to vibrate ultrasonically. The ultrasonic treatment is applied by applying vibrations at a frequency of 0.5 MHz to 10 MHz. The ultrasonic output is set to 0.1 W / cm. 2 ~1.0W / cm 2 The ultrasonic treatment can be applied by setting the ultrasonic frequency to 100 kHz. The ultrasonic treatment can bring about multiple effects. For example, it can burn fat, promote penetration and / or absorption of active ingredients for the skin, provide a cleansing effect, and generate frictional heat. In particular, when applied together with iontophoresis, it can promote penetration and / or absorption of active ingredients for the skin. By configuring the probe to be connected to or integrated with an ultrasonic vibrator, ultrasonic waves can be applied to the skin.

[0036] Ultrasonic treatment and iontophoresis may be applied sequentially, alternately, or simultaneously, and it is preferable to apply ultrasonic treatment and iontophoresis simultaneously in order to enhance the penetration and / or absorption of the active ingredient for skin.

[0037] All documents mentioned herein are incorporated by reference in their entirety.

[0038] The following examples of the present invention are for illustrative purposes only and do not limit the technical scope of the present invention. The technical scope of the present invention is limited only by the claims. The present invention may be modified, for example, by adding, deleting, or substituting components of the present invention, provided that the modifications do not depart from the spirit of the present invention. [Example]

[0039] Example 1: Pore improvement effect of the cosmetic method of the present invention The pore-improving effect of the cosmetic method of the present invention was evaluated visually and based on photographs for 12 female subjects aged 30 to 50. The pore-improving effect of the cosmetic method of the present invention was also evaluated based on subjective evaluation. (1) The cosmetic method of the present invention An aqueous solution containing 2.0% glycylglycine and 1% polyoxyethylene (14) polyoxypropylene (7) dimethyl ether (pH 6.0) was prepared as the test solution. 2 , 1.5 kHz) and ultrasound (3.0 MHz) were applied. (2) Visual and photographic pore evaluation Visual pore evaluation was performed by an experienced researcher, who visually evaluated the pores on the right and left areas of the face. Similarly, photographic pore evaluation was performed by taking photographs of the face and evaluating the pores based on the photographs. The pore evaluation criteria were based on Table 1, and a pore score was calculated. Thereafter, the above-mentioned test solution was applied once a day for four weeks, and iontophoresis (2.0 mA / cm 2 , 1.5 kHz) and ultrasound (3.0 MHz) were applied to the entire face for 10 minutes. Visual pore evaluation was performed on the start day of the study and at weeks 1, 2, and 4, and the scores were averaged. The change in visual pore evaluation from the start day is also shown (Tables 2 and 3). [Table 1]

[0040] [Table 2]

[0041] [Table 3]

[0042] (3) Subjective pore evaluation Subjective pore evaluation was performed by questionnaire. The pore evaluation criteria were based on Table 4, and a pore score was calculated. After that, the test solution was applied once a day for 4 weeks, and iontophoresis (2.0 mA / cm 2 A 1.5 kHz (1.5 kHz) and an ultrasound (3.0 MHz) were applied to the entire face for 10 minutes. Pore size was visually evaluated on the first day of the study and at weeks 1, 2, and 4, and the scores were averaged. The change in score from the first day is also shown (Table 5). [Table 4]

[0043] [Table 5]

[0044] Example 2: Test of penetration of active ingredients into the skin using the cosmetic method of the present invention Four test areas were set up on the forearms of five subjects. Aqueous Solution 1 was prepared containing glycylglycine (2.0%), a vitamin C derivative (0.5%), and ion-exchanged water (97.5%), while Aqueous Solution 2 was prepared containing glycylglycine (0.5%), a vitamin C derivative (0.5%), and ion-exchanged water (99%). Tests were carried out on four test areas as shown in Table 6. Aqueous Solution 1 and Aqueous Solution 2 were used at a concentration of 1.8 mL / cm 2 The amount of the solution was applied to the area and spread over the entire area by applying a probe. In areas 3 and 4, a penetration treatment was carried out over 3 minutes while applying iontophoresis and ultrasound from the probe. In area 2, the solution was only spread over 3 minutes by applying a probe without applying iontophoresis or ultrasound. Iontophoresis (2.0 mA / cm 2 The skin was then treated with 1.5 kHz (1.5 kHz) and ultrasound (3.0 MHz) for 10 minutes. The treatment was left for a certain period of time to allow the drug to penetrate. The skin was then washed, and a stratum corneum sample was obtained by tape stripping. The amount of glycylglycine in the stratum corneum sample was measured using liquid chromatography-mass spectrometry (LC / MS). The results are shown in Figure 1. [Table 6]

Claims

1. applying to the skin a composition containing a skin active ingredient having a molecular weight of 100 to 1000 Da; and applying iontophoresis and ultrasound simultaneously from the negative electrode side to the skin after application.

2. 2. The cosmetic method according to claim 1, wherein the active ingredient for skin is an amino acid derivative, or glycyrrhizinic acid or a salt thereof, or a derivative thereof.

3. The cosmetic method according to claim 1 , wherein the amino acid derivative is a glycine derivative or a salt thereof.

4. The cosmetic method according to claim 3, wherein the glycine derivative is glycylglycine.

5. The cosmetic method according to claim 1 , wherein the cosmetic method promotes penetration of the active ingredient for skin into the stratum corneum.

6. The cosmetic method according to claim 1 for pore care.

7. The cosmetic method according to claim 1, wherein the composition further comprises 2.0 to 50% polyhydric alcohol as a moisturizer.

8. The cosmetic method according to claim 7, wherein the polyhydric alcohol is a dihydric or trihydric alcohol.

9. 7. The method of claim 6, wherein the polyhydric alcohol is 1,3 butylene glycol or dipropylene glycol.

10. The cosmetic method according to claim 1, wherein the composition further comprises 0.1 to 10% of a block copolymer comprising a lipophilic portion and a hydrophilic portion as a penetration enhancer.

11. 2. The cosmetic method according to claim 1, wherein the output of the iontophotorhesis is 0.1 mA to 2.5 mA, and the frequency of the ultrasonic waves is 0.5 MHz to 10 MHz.

12. Ultrasonic output is 0.1 W / cm 2 ~1.0 W / cm 2 The cosmetic method according to claim 1,

13. A cosmetic composition for use in conjunction with iontophoresis and ultrasound applications, said cosmetic composition comprising a skin active ingredient having a molecular weight of 100 to 1000 Da.

14. The cosmetic composition according to claim 13, wherein the active ingredient for skin is an amino acid derivative, or glycyrrhizinic acid or a salt thereof, or a derivative thereof.

15. The cosmetic composition according to claim 13, wherein the active ingredient for skin is a glycine derivative or a salt thereof.

16. The cosmetic composition according to claim 13, further comprising at least one component selected from the group consisting of a penetration enhancer, a moisturizer, and an oil.

17. 0.01-10% glycylglycine 0.1-10% penetration enhancer 2.0-50% moisturizer The cosmetic composition of claim 13, comprising:

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