Oral composition

Abstract

To provide an oral composition having a cartilage protection effect and enhanced taste.SOLUTION: An oral composition includes glucosamine, young barley leaves, soluble dietary fiber, oligosaccharides, and lactic acid bacteria. Alternatively, the oral composition includes glucosamine, hyaluronic acid, young barley leaves, soluble dietary fiber, oligosaccharides, and lactic acid bacteria.SELECTED DRAWING: None

Description

[Technical Field]

[0001] The present invention relates to an oral composition containing glucosamine, young barley leaves, water-soluble dietary fiber, oligosaccharides, and lactic acid bacteria. The present invention also relates to an oral composition containing glucosamine, hyaluronic acid, young barley leaves, water-soluble dietary fiber, oligosaccharides, and lactic acid bacteria. [Background technology]

[0002] Conventionally, a food composition containing young barley leaves and glucosamine and having anti-inflammatory effects has been known (see, for example, Patent Document 1). [Prior art documents] [Patent documents]

[0003] [Patent Document 1] Japanese Patent Application Laid-Open No. 2011-055712 Summary of the Invention [Problem to be solved by the invention]

[0004] However, these effects are not sufficient, and further improvements are needed. In addition, there is a problem that the flavor of young barley leaves deteriorates when combined with glucosamine, and therefore further improvements are needed.

[0005] An object of the present invention is to provide an oral composition containing glucosamine, young barley leaves, etc., which has excellent cartilage protecting effects and taste improving effects. [Means for solving the problem]

[0006] The present inventors have discovered that a combination of glucosamine, young barley leaves, water-soluble dietary fiber, oligosaccharides, and lactic acid bacteria provides an excellent cartilage-protecting effect and a taste-improving effect, leading to the completion of the present invention.Furthermore, the present inventors have discovered that a combination of glucosamine, hyaluronic acid, young barley leaves, water-soluble dietary fiber, oligosaccharides, and lactic acid bacteria provides an excellent cartilage-protecting effect and a taste-improving effect, leading to the completion of the present invention.

[0007] That is, the present invention is as follows. [1] An oral composition containing glucosamine, young barley leaves, water-soluble dietary fiber, oligosaccharides, and lactic acid bacteria. [2] An oral composition containing glucosamine, hyaluronic acid, young barley leaves, water-soluble dietary fiber, oligosaccharides, and lactic acid bacteria. [3] The oral composition according to [1] or [2] above, further comprising at least one selected from collagen and proteoglycan. [4] The oral composition according to [1] or [2] above, wherein the amount of young barley leaves blended is 10% by mass or more. [5] The oral composition according to [1] or [2] above, wherein the water-soluble dietary fiber is indigestible dextrin. [6] The oral composition according to [1] or [2] above, wherein the oligosaccharide is at least one kind of oligosaccharide selected from those having three or more sugars. [7] The oral composition according to [1] or [2] above, wherein the lactic acid bacteria is at least one species of lactic acid bacteria selected from the genera Bifidobacterium and Bacillus. [Effects of the Invention]

[0008] According to the present invention, an oral composition containing glucosamine and young barley leaves, etc., can be provided that has an excellent cartilage protective effect and improves walking function in people with impaired mobility. Furthermore, according to the present invention, an oral composition can be provided in which the taste, such as the deterioration of the flavor of young barley leaves, that occurs when young barley leaves are combined with glucosamine is improved. DETAILED DESCRIPTION OF THE INVENTION

[0009] The oral composition of the present invention is characterized by containing glucosamine, young barley leaves, water-soluble dietary fiber, oligosaccharides, and lactic acid bacteria.The present invention also relates to an oral composition containing glucosamine, hyaluronic acid, young barley leaves, water-soluble dietary fiber, oligosaccharides, and lactic acid bacteria.

[0010] The oral composition of the present invention exhibits an excellent cartilage-protecting effect. Specifically, the oral composition of the present invention improves the cell activation activity of normal human knee articular chondrocytes and has the effect of promoting hyaluronic acid absorption, thereby exhibiting an excellent cartilage-protecting effect. Furthermore, because of its excellent cartilage-protecting effect, it exhibits an effect of improving walking function in people with impaired mobility.

[0011] Therefore, the oral composition of the present invention can be used as a composition for protecting cartilage, a composition for hyaluronic acid absorption, a composition for improving walking function, etc.

[0012] Furthermore, by combining glucosamine, young barley leaves, water-soluble dietary fiber, etc., the oral composition of the present invention can provide an oral composition containing glucosamine and young barley leaves that has an improved taste and is easy to drink.

[0013] Hereinafter, each component contained in the oral composition of the present invention (hereinafter, sometimes referred to as the component of the present invention) will be described. [Glucosamine] The glucosamine of the present invention can be glucosamine, its salts, or N-acetylglucosamine. Examples of salts include hydrochloride, sulfate, and phosphate. Such glucosamine may be derived from a living organism or chemically synthesized. The glucosamine used in the oral composition of the present invention is not particularly limited as long as it is commonly available. In the present invention, N-acetylglucosamine is preferably used, as it can enhance the effects of the present invention. N-acetylglucosamine is a monosaccharide and is a structural unit (monomer) of chitin, which is found in the outer coverings of crustaceans such as shrimp and crab, and insects such as beetles and crickets, and in the cell walls of fungi. N-acetylglucosamine can be prepared, for example, by partially hydrolyzing chitin, a polysaccharide prepared from the shells of crustaceans such as crabs and shrimp, with acid and further decomposing it with an enzyme such as chitinase. Commercially available N-acetylglucosamine can be used in the present invention.

[0014] [Young barley leaves] Barley (Hordeum vulgare L.) is an annual or biennial herb belonging to the Poaceae family, believed to be native to Central Asia, and is broadly classified into two-row barley, six-row barley, etc., depending on the panicle shape. The barley leaves used in the oral composition of the present invention are not particularly limited as long as they are commonly available, and any variety of barley leaves, such as two-row barley or six-row barley, may be used. Furthermore, the barley leaves may also include stems.

[0015] In the present invention, for example, pulverized barley leaves, squeezed juice, extracts, etc. can be used. Examples of pulverized barley leaves include dried powder, shredded barley leaves and their dried products (dried shredded barley leaves), granules, etc. The squeezed juice and extract may be in liquid form, but can also be used as a paste or dried powder (juice powder, extract powder). The extract can be obtained by extraction using an appropriate solvent, and examples of the solvent that can be used include water (warm water, hot water), ethanol, and aqueous ethanol. In the present invention, it is preferable to use dried powder of barley leaves (pulverized barley leaves powder) in order to further enjoy the effects of the present invention.

[0016] [Water-soluble dietary fiber] Water-soluble dietary fiber refers to water-soluble indigestible components (dietary fiber) contained in food that cannot be digested by human digestive enzymes. The water-soluble dietary fiber in the present invention is not particularly limited, and examples thereof include indigestible dextrin, pectin, guar gum, guar gum hydrolysate (enzymatic hydrolysate of guar bean), agarose, glucomannan, polydextrose, alginic acid and its salts, β-glucan, inulin, carrageenan, fucoidan, and derivatives thereof. These can be used alone or in combination of two or more, and it is preferable to use in combination of two or more. In the present invention, it is preferable to use indigestible dextrin in order to further enjoy the effects of the present invention.

[0017] [Oligosaccharides] The term "oligosaccharide" as used herein refers to an oligosaccharide, a compound formed by glycosidic bonds between three to twenty sugars. Specific examples of oligosaccharides used herein include, but are not limited to, indigestible oligosaccharides such as lactoferrin oligosaccharides, fructooligosaccharides, galactooligosaccharides, xylooligosaccharides, and soybean oligosaccharides, and digestible oligosaccharides such as maltooligosaccharides, isomaltooligosaccharides, beet oligosaccharides (raffinose), lactulose, and lactitol. The number of sugars constituting the oligosaccharide is preferably from three to eighteen sugars, more preferably from three to fourteen sugars, even more preferably from three to decasaccharides, and particularly preferably from three to heptasaccharides.

[0018] In the present invention, isomaltooligosaccharides or beet oligosaccharides (raffinose) are preferably used in order to obtain the effects of the present invention more effectively. It is also preferable to combine two or more types of oligosaccharides, and it is more preferable to combine two or more types of digestible oligosaccharides. The physiological functions of indigestible oligosaccharides include being able to reach the large intestine without being degraded by human digestive enzymes, contributing to the activation and proliferation of intestinal bifidobacteria, and improving the intestinal environment, while the physiological functions of digestible oligosaccharides include being able to be degraded by human digestive enzymes, absorbed in the stomach and small intestine, and used as an energy source.

[0019] [Lactic acid bacteria] In the present invention, lactic acid bacteria refer collectively to bacteria that produce lactic acid through metabolism, including bifidobacteria, and examples of lactic acid bacteria include those of the genera Bifidobacterium, Lactobacillus, Enterococcus, Leuconostoc, Pediococcus, Staphylococcus, Tetragenococcus, Leuconostoc, Pediococcus, Staphylococcus, Tetragenococcus, and Bacillus. Bifidobacterium genus includes Bifidobacterium bifidum, Bifidobacterium breve, Bifidobacterium infantis, Bifidobacterium lactis, Bifidobacterium longum, Bifidobacterium adolescentis, Bifidobacterium mongoliense, Lactbacillus genus includes Lactbacillus brevis, Lactbacillus gasseri, Lactobacillus acidophilus, Lactobacillus buchneri, Lactobacillus bulgaricus, Lactobacillus delburvecki, Lactobacillus casei, Lactobacillus crispatus, Lactobacillus curvatus, Lactobacillus halivaticus, Lactobacillus pentosus, Lactobacillus plantarum, Lactobacillus paracasei, Lactobacillus rhamnosus, Lactobacillus salivarius, Lactobacillus sporogenes, Lactobacillus sakei, Lactobacillus fructivorans, Lactobacillus hilgardii, Lactobacillus reuteri, Lactobacillus fermentum, Enterococcus faecalis(sometimes referred to as Streptococcus faecalis), Enterococcus faesium (sometimes referred to as Streptococcus faesium), the genus Streptococcus includes Streptococcus thermophilus and Lactococcus lactis (sometimes referred to as Streptococcus lactis), the genus Leuconostoc includes Leuconostoc mesenteroides and Leuconostoc oenos, the genus Pediococcus includes Pediococcus acidilactici and Pediococcus pentosaceus, the genus Staphylococcus includes Staphylococcus carnosus and Staphylococcus xylosus, the genus Tetragenococcus includes Tetragenococcus halophilus, and the genus Bacillus includes Bacillus coagulans and Bacillus mesentericus. These lactic acid bacteria can be used singly or in combination of two or more, preferably in combination of two or more. The method for obtaining the lactic acid bacteria is not particularly limited, and for example, lactic acid bacteria isolated from foods such as yogurt or vegetables, or commercially available products can be used.

[0020] In the present invention, lactic acid bacteria of the genus Bifidobacterium and Bacillus are preferred, and Bifidobacterium longum and Bacillus coagulans (spore-forming lactic acid bacteria) are particularly preferred, as they allow for greater enjoyment of the effects of the present invention.

[0021] [Hyaluronic acid] The hyaluronic acid in the present invention is a kind of mucopolysaccharide present in the skin, joints, vitreous body of the eyeball, etc. of humans and vertebrates, and is also referred to as a compound known as hyaluronan.The hyaluronic acid used in the present invention is not particularly limited, and can be derived from cockscomb, derived from microorganisms, synthetic products, or hydrolyzed with enzymes.The hyaluronic acid in the present invention includes the salts of hyaluronic acid such as sodium hyaluronate and potassium hyaluronate, and also includes the decomposition products of hyaluronic acid that have been reduced in molecular weight, such as hydrolyzed hyaluronic acid.As the hyaluronic acid of the present invention, sodium hyaluronate is particularly preferred, because it can better enjoy the effects of the present invention.In addition, the molecular weight of the hyaluronic acid used in the present invention varies depending on the number and type of repeating unit, and is not limited. The average molecular weight (weight average molecular weight) of the hyaluronic acid in the present invention is not particularly limited, but is preferably 100 to 100,000, more preferably 500 to 50,000, and particularly preferably 1,000 to 10,000, since a higher effect can be obtained.

[0022] [collagen] The oral composition of the present invention preferably contains collagen in addition to the above-described components of the present invention. Collagen in the present invention refers to a type of protein present in the cartilage tissues, bones, organs, tendons, etc. of mammals such as pigs, birds such as chickens, fish such as sharks and rays, and mollusks such as squid. Such collagen includes collagen protein, collagen peptides obtained by hydrolyzing collagen protein, and atelocollagen, which is obtained by treating collagen molecules with protease to remove the telopeptide portion. The collagen in the present invention can be animal-derived or synthetic, and is not particularly limited. However, type II collagen is preferred to enhance the effects of the present invention. Type II collagen may be hydrolyzed. The average molecular weight (weight-average molecular weight) of the collagen in the present invention is not particularly limited, but is preferably 100 to 100,000, more preferably 500 to 50,000, and particularly preferably 1,000 to 10,000, in order to obtain even greater effects.

[0023] [Proteoglycan] The oral composition of the present invention preferably contains a proteoglycan in addition to the above-described components of the present invention. The proteoglycan of the present invention refers to a complex polysaccharide that is abundant in animal tissues, particularly cartilage tissues, and has a protein core to which glycosaminoglycans such as chondroitin sulfate and dermatan sulfate are covalently bonded. It is also known that proteoglycans exist in vivo in a structure in which the core protein is further bound to hyaluronic acid, and their molecular weights are as large as tens of thousands to tens of millions. Specific examples of the proteoglycan used in the present invention include animal-derived proteoglycans, with salmon nasal cartilage-derived proteoglycans being particularly preferred. Commercially available products can also be used.

[0024] In the oral composition of the present invention, in order to obtain the effects of the present invention more effectively, the amount of glucosamine contained is preferably 0.01% by mass or more, more preferably 0.1% by mass or more, and even more preferably 1 to 10% by mass.

[0025] Furthermore, the amount of young barley leaves in the oral composition of the present invention is preferably 5% by mass or more, more preferably 10% by mass or more, and even more preferably 15 to 50% by mass, in order to better enjoy the effects of the present invention.

[0026] Furthermore, the amount of hyaluronic acid in the oral composition of the present invention is preferably 0.00001% by mass or more, more preferably 0.0001% by mass or more, and even more preferably 0.001 to 1% by mass, in order to better enjoy the effects of the present invention.

[0027] The blending mass ratio of the three components of the present invention other than glucosamine, hyaluronic acid, and young barley leaves is, for example, 1:100 to 0.0001 for water-soluble dietary fiber and oligosaccharide, preferably 1:50 to 0.001, and more preferably 1:25 to 0.01 for water-soluble dietary fiber and lactic acid bacteria, for example, 1:15 to 0.00001, preferably 1:10 to 0.0001, and more preferably 1:1 to 0.001 for water-soluble dietary fiber and lactic acid bacteria, for example, 1:5 to 0.00001, preferably 1:2.5 to 0.0001, and more preferably 1:1 to 0.001 for lactic acid bacteria and oligosaccharide.

[0028] Furthermore, when the oral composition of the present invention contains collagen, the amount of collagen added is preferably 0.00001% by mass or more, more preferably 0.0001% by mass or more, and even more preferably 0.001 to 1% by mass, in order to better enjoy the effects of the present invention.

[0029] Furthermore, when the oral composition of the present invention contains proteoglycan, the amount of proteoglycan to be incorporated is preferably 0.0001% by mass or more, more preferably 0.001% by mass or more, and even more preferably 0.01 to 1% by mass, in order to better enjoy the effects of the present invention.

[0030] The intake amount of the oral composition of the present invention is not particularly limited, but in order to enjoy the effects of the present invention more effectively, it is preferable that the daily intake amount be 2 g / day or more, more preferably 3 g / day or more, and even more preferably 4 g / day or more. The oral composition of the present invention can be stored as a daily amount in a single container or divided into, for example, 2 to 3 multiple containers so that the daily intake amount is the above-mentioned intake amount.

[0031] As described above, the oral composition of the present invention can be used as a composition for protecting cartilage, a composition for hyaluronic acid absorption, a composition for promoting hyaluronic acid absorption, a composition for improving walking function, etc., and can be used as so-called health foods such as functional foods whose efficacy has been approved by a designated organization, such as specified health foods, nutritional functional foods, and functional food, or as pharmaceuticals (including quasi-drugs).

[0032] The chondroprotective composition of the present invention is not particularly limited as long as it contains the components of the present invention and can be distinguished from other products in that it is used for cartilage protection, and for example, the scope of the present invention includes products that claim to have an intestinal regulating effect on the product itself, packaging, instructions, or promotional materials (advertising media).Furthermore, functional food products include those that use a chondroprotective effect as the scientific basis for their functionality.

[0033] The cartilage-protecting composition of the present invention is not limited to those labeled with the component of the present invention as the active ingredient on the product packaging, etc. For example, it may be a product in which the active ingredient is not specified. Furthermore, even general foods that are manufactured and sold with a suggested use are included within the scope of the present invention. For example, foods sold with personal testimonials of ingestors mentioning the cartilage-protecting effect listed on their websites, etc., as personal impressions, are also included within the scope of the present invention.

[0034] Specific examples of the cartilage-protecting composition of the present invention include so-called health foods labeled with such slogans as "For active daily life," "Move energetically," "Supplement cartilage components," "No need to hold back on walking, sitting, or moving," "For smooth daily life," "For those concerned about standing and sitting," "Joint support," "Cartilage support," "Contains cartilage components," "For those who want to take nutritional components and cartilage components together," "For those who want to take glucosamine in a delicious way," "When you can't keep up with glucosamine supplements," "For those concerned about the number of tablets in a glucosamine supplement," "For those concerned about active balance," and "For those who want to supplement cartilage components that decrease with age."

[0035] Examples of the form of the oral composition of the present invention include tablets, capsules, powders, granules, liquids, particles, rods, plates, blocks, solids, rounds, pastes, creams, caplets, gels, jellies, chewable tablets, and sticks. Among these, tablets, capsules, powders, granules, liquids, and jellies are preferred because they can better enjoy the effects of the present invention. Specific examples include supplements, packaged beverages filled in PET bottles, cans, jars, etc., instant drinks (powdered drinks) that can be dissolved in water (hot water), milk, fruit juice, green juice, etc., and jelly-like foods and beverages. These are preferred because they are easy to drink during meals and can enhance palatability.

[0036] The oral composition of the present invention can be prepared by adding other ingredients than those mentioned above, if necessary, and by a known method such as mixing.

[0037] The oral composition of the present invention is particularly preferably used as a granulated product. Granulated compositions have improved dispersibility in liquids and ease of drinking, making them suitable for use as powdered beverages, and can be used as granulated beverage products. A granulated beverage product refers to a granulated product (a powdered beverage product that is a granulated product) that is mixed with a liquid such as water, hot water, milk, or soy milk by a consumer at the time of ingestion and consumed as a beverage.

[0038] The granulation method is not particularly limited and may be selected from granulation methods commonly used by those skilled in the art. Examples include fluidized bed granulation, extrusion granulation, tumbling granulation, and stirring granulation. Regardless of the granulation method, a granulated product can be produced using any commercially available granulation device by appropriately setting various production conditions such as the drying temperature. From the viewpoint of obtaining a granulated product with excellent ease of granulation and dispersibility, fluidized bed granulation is particularly preferred. Fluidized bed granulation is a method in which raw material powder particles are fluidized by blowing air on them with an air flow, and then a binding liquid such as water is sprayed onto them to bind the powder particles together and form a granulated product, which is an agglomerate of the powder particles. [Example]

[0039] The present invention will be described in more detail below based on examples, but the present invention is not limited to these examples. [Test 1] The composition of the present invention was used to confirm the cell-activating activity of normal human knee articular chondrocytes (NHAC-kn). Normal human knee articular chondrocytes are an excellent model system for cartilage formation and are widely used in research on osteoarthritis, joints, chondrocyte differentiation, hyaline cartilage, etc. By confirming its cell-activating activity, it is possible to confirm the cartilage-protecting effect and the walking function-improving effect by maintaining and improving cartilage tissue.

[0040] The test substances in this experiment are as follows: The glucosamine used was commercially available N-acetylglucosamine extracted from shrimp and crab shells by acid or alkali treatment.

[0041] The barley leaves used were dried and crushed powder of young leaves (manufactured by Toyo Shinyaku Co., Ltd.) Specifically, fresh young leaves were cut, blanched, dried, crushed, and passed through a sieve with openings of 1 mm or less.

[0042] As the water-soluble dietary fiber, we used commercially available indigestible dextrin made from tapioca starch, with a dietary fiber content of 38-45% as measured by enzyme-liquid chromatography.

[0043] As oligosaccharides, commercially available beet oligosaccharide (raffinose) and isomaltooligosaccharide were used.

[0044] The lactic acid bacteria used were commercially available Bifidobacterium longum and Bacillus coagulans (spore-forming lactic acid bacteria). Specifically, the Bifidobacterium longum was a dry powder of live bacteria with a bacterial count of 150 billion or more per gram, and the Bacillus coagulans (spore-forming lactic acid bacteria) was a dry bacterial cell with a lactobacillus spore count of 6 billion or more per gram.

[0045] The hyaluronic acid used was commercially available sodium hyaluronate, specifically low-molecular-weight sodium hyaluronate with an average molecular weight of 3,750, extracted and purified from sodium hyaluronate obtained by fermentation. The collagen used was a commercially available type II collagen peptide, specifically, type II collagen peptide obtained by enzymatic hydrolysis of chicken cartilage. The proteoglycan used was a commercially available proteoglycan extracted from salmon nasal cartilage.

[0046] The specific experimental procedures are as follows. (1) In a 37°C, 5% CO2 incubator, 75 cm 2 Commercially available normal human knee articular chondrocytes were cultured in flasks using chondrocyte growth medium (manufactured by LONZA).

[0047] (2) Cells suspended by trypsin treatment were collected at 75 cm 2 From flask to 1 x 10 cells in a 96-well plate 4 The cells were seeded at a density of 100 cells / well and pre-cultured for 24 hours in a 5% CO2 incubator at 37°C.

[0048] (3) After removing the medium, the test substances were mixed at the ratios shown in Table 1 below, and 100 μL / well of the medium containing the test substances was added. The cells were cultured at 37°C in a 5% CO2 incubator for 24 hours. A chondrocyte growth medium was used as the medium, and the total concentration of the test substances in the medium was 10 μg / mL.

[0049] (4) After 24 hours of culture, the medium was removed and the cells were washed with PBS. (5) 150 μL of Cell Counting Kit-8 (Dojindo Laboratories) diluted 30-fold with serum-free DMEM was added to each well, and the wells were left to stand in a 37°C, 5% CO2 incubator to adequately stain the cells, and the absorbance at 450 nm was measured.

[0050] (6) Based on the obtained data, the % of control was calculated, and the results of evaluating the cell activation activity for each Example and Comparative Example are shown in Table 1 as relative values ​​with the value for Comparative Example 1 set at 100.

[0051] % of control = (Data sample - Data blank) / (Data control - Data blank) × 100

[0052] [Table 1]

[0053] As shown in Table 1, the compositions of the examples containing five to eight components of the present invention exhibited synergistically improved cell activation activity compared to the comparative compositions not containing some of the components of the present invention. Specifically, when comparing Comparative Example 1, which contained two components and did not contain water-soluble dietary fiber, lactic acid bacteria, or oligosaccharides, with Example 1, which contained five components and also contained water-soluble dietary fiber, lactic acid bacteria, and oligosaccharides, the cell activation activity of Example 1 was improved. Similarly, when comparing the results of Examples 1 and 2, the addition of sodium hyaluronate further improved the cell activation activity. Similarly, when comparing the results of Examples 2 and 3, the addition of type II collagen further improved the cell activation activity. Furthermore, when comparing the results of Examples 3 and 4, the addition of proteoglycan particularly improved the cell activation activity. The composition of the present invention improved the cell activation activity of normal human knee articular chondrocytes, an excellent model system for cartilage formation, and was therefore confirmed to exhibit cartilage protection effects and walking function improvement effects by maintaining and improving cartilage tissue.

[0054] [Test 2] The composition of the present invention was used to confirm the hyaluronic acid absorption promoting effect by the monolayer permeability of human colon cancer derived cell line.The monolayer permeability of human colon cancer derived cell line is a common in vitro model used to evaluate the absorption of compounds through the human intestine.By confirming the permeability of hyaluronic acid, the main component of synovial fluid, it is possible to confirm the effect of promoting the absorption of hyaluronic acid, which has the lubricating effect of articular cartilage (improving the sliding between bones), and thus maintain and / or improve the movement of joints, thereby improving walking function.

[0055] The test substances used in this experiment were the same as those used in Test 1.

[0056] The specific experimental procedures are as follows. (1) In a 37°C, 5% CO2 incubator, 75 cm 2 Commercially available human colon cancer cells were cultured in flasks using 10% FBS-1% NEAA-DMEM medium.

[0057] (2) Trypsinized cells were suspended and placed in insert wells of a 24-well plate at 4 × 10 4 The cells were seeded at a density of 100 cells / well and pre-cultured for 24 hours in a 5% CO2 incubator at 37°C. (3) After 24 hours, the medium was changed, and the cells were then cultured for 21 days, with the medium changed every 3 to 4 days, to form a monolayer. (4) After the monolayer was formed, the electrical resistance was measured and the plate was washed with HBSS buffer.

[0058] (5) Test substances were mixed at the ratios shown in Table 2 below, and 50 μL / well of medium containing the test substances was added. The cells were cultured for 4 hours in a 5% CO2 incubator at 37°C. HBSS buffer was used as the medium, and the total concentration of the test substances in the medium was 1000 μg / mL.

[0059] (6) The amount of hyaluronic acid in the permeate was measured using a commercially available kit, and the amount of hyaluronic acid absorbed was shown in Table 2 as a relative value with the value of Comparative Example 2 set at 100.

[0060] [Table 2]

[0061] As shown in Table 2, the compositions of the examples containing 6 to 8 components of the present invention synergistically improve hyaluronic acid absorption compared with the comparative compositions that do not contain some of the components of the present invention.Specifically, when comparing Comparative Example 2, which contains two components of barley young leaves and hyaluronic acid Na, with Example 5, which contains six components, including water-soluble dietary fiber, lactic acid bacteria, oligosaccharides and N-acetylglucosamine, the hyaluronic acid absorption in Example 5 is improved.Similarly, when comparing the results of Example 5 and Example 6, adding type II collagen further improves collagen absorption.Similarly, when comparing the results of Example 6 and Example 7, adding proteoglycan particularly improves hyaluronic acid absorption. The composition of the present invention improved the monolayer permeability of hyaluronic acid in a human colon cancer-derived cell line, which is a common in vitro model used to evaluate the absorbability of compounds through the human intestine. This confirmed that the composition of the present invention exhibits an effect of improving walking function by maintaining and / or improving joint movement through the lubricating effect of articular cartilage (improving the sliding between bones) caused by promoting hyaluronic acid absorption.

[0062] [Test 3] The taste-improving effect of the composition of the present invention was confirmed. The test substance used in this experiment was the same as that used in Example 1. As an excipient that does not affect taste, commercially available powdered dextrin was used.

[0063] Each sample was obtained by mixing 4 g of powder sample with 150 ml of water according to the blending ratios shown in Table 3. Of these samples, the sample obtained from the powder sample of Comparative Example 3 was used as the standard. The sensory evaluation was conducted by panelists who regularly consume green juice and have experience in sensory evaluation. The panelists were asked to evaluate the green smell, fishy smell, mellowness, bitterness, and crispness of the aftertaste of the young barley leaves.

[0064] <The green smell of young barley leaves> ◎: There is no green smell of young barley leaves and it tastes much better than the standard product. ○: The green smell of young barley leaves is less, and it tastes better than the standard product. △: Similar to the standard product. ×: Below standard.

[0065] <Fishy smell> ◎: No fishy smell and feels much easier to drink than the standard product. ○: It has less fishy smell and is easier to drink than the standard product. △: Similar to the standard product. ×: Below standard.

[0066] <Smoothness> ◎: It has a smooth texture and is much easier to drink than the standard product. ○: It has a mellow taste and feels easier to drink than the standard product. △: Similar to the standard product. ×: Below standard.

[0067] <Bitterness> ◎: No bitterness, and leaves your mouth feeling much cleaner than the standard product. ○: Less bitterness and feels cleaner than the standard product. △: Similar to the standard product. ×: Below standard.

[0068] <Clear aftertaste> ◎: The aftertaste is crisp and leaves your mouth feeling much cleaner than the standard product. ○: It has a crisp aftertaste and feels cleaner than the standard product. △: Similar to the standard product. ×: Below standard.

[0069] "comprehensive evaluation" The overall evaluation was made based on the evaluation of the green smell, fishy smell, mellowness, bitterness, and clean aftertaste of the young barley leaves, and was determined according to the following criteria. The evaluation results are shown in Table 3 above. ◎: Excellent, with neither × nor △. ◯: No X or △, good. △: There are △ marks but no × marks, so it is acceptable. ×: There is one or more ×s, and it is not possible.

[0070] [Table 3]

[0071] As shown in Table 3, the compositions of the examples containing five to eight components of the present invention had improved taste compared to the comparative compositions not containing some of the components of the present invention. Specifically, when the results of Comparative Example 3, which contained only young barley leaves, were compared with the results of Comparative Example 4, which also contained N-acetylglucosamine, Comparative Example 3 received worse ratings in all categories. Furthermore, when the results of Comparative Example 3, which contained only young barley leaves, were compared with the results of Comparative Example 5, which contained young barley leaves and indigestible dextrin, which is known to have a taste-improving effect, Comparative Example 5 showed improved mellowness, but the crispness of the aftertaste was poorer, and other evaluation items such as grassy smell were similar to those of Comparative Example 3, indicating that the improvement effect was insufficient. On the other hand, Example 8, which contained the five components of the present invention, was superior in all evaluation items compared with the results of Comparative Examples 2 and 3. Furthermore, when the results of Examples 8 to 11 were compared, Example 9, which further added sodium hyaluronate to the components of Example 8, had less bitterness and a more improved taste than Example 8. And, Example 10, which further added type II collagen to the ingredients of Example 9, had less of a grassy smell, was mellower, and had a more improved taste than Example 9. Furthermore, Example 11, which further added proteoglycan to the ingredients of Example 10, was found to be a composition that was particularly excellent in evaluations of all items and was easy to drink.

[0072] [Production Examples 1 to 3] (Production of powdered beverages) As shown in Table 4, glucosamine was mixed with other ingredients, the composition was granulated, and a powdered beverage was produced. When 5 g of the resulting powdered beverage was mixed with 150 mL of water and ingested, it exhibited a cartilage-protecting effect and was easy to drink.

[0073] [Table 4] [Industrial Applicability]

[0074] The oral composition of the present invention can be used as a health food or the like, and is therefore highly useful industrially.

Claims

1. An oral composition containing glucosamine, young barley leaves, water-soluble dietary fiber, oligosaccharides, and lactic acid bacteria.

2. An oral composition containing glucosamine, hyaluronic acid, young barley leaves, water-soluble dietary fiber, oligosaccharides, and lactic acid bacteria.

3. The oral composition according to claim 1 or 2, further comprising at least one selected from collagen and proteoglycan.

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