Topical preparation for skin

Abstract

To provide a novel application of diamond nanoparticles and platinum nanoparticles.SOLUTION: A topical preparation for skin, comprising diamond nanoparticles, platinum nanoparticles, and an organic sulfur compound. A fascia relaxation agent, comprising the topical preparation for skin. An agent for improving negative mood state, comprising the topical preparation for skin. An agent for enhancing body flexibility, comprising the topical preparation for skin.SELECTED DRAWING: None

Description

[Technical Field]

[0001] The present invention relates to an external preparation for skin. [Background technology]

[0002] Nanoparticles that emit far-infrared rays and cause electron transfer based on plasmon resonance, such as diamond nanoparticles and platinum nanoparticles, are known to improve sleep quality when used in clothing, for example (see, for example, Patent Document 1). [Prior art documents] [Patent documents]

[0003] [Patent Document 1] International Publication No. 2021 / 245969 Summary of the Invention [Problem to be solved by the invention]

[0004] The present invention aims to further utilize diamond nanoparticles and platinum nanoparticles and to provide new uses for these particles. [Means for solving the problem]

[0005] The present inventors have discovered that adding an organic sulfur compound to diamond nanoparticles and platinum nanoparticles can prepare topical skin preparations that can exert various effects. The present invention is based on these findings.

[0006] The present invention includes the following aspects. [1] A topical skin preparation containing diamond nanoparticles, platinum nanoparticles, and organic sulfur compounds. [2] The topical skin preparation according to [1], wherein the organic sulfur compound is methylsulfonylmethane. [3] The topical skin preparation according to [1] or [2], wherein the content of diamond nanoparticles is 10 mass% or less based on the total amount of the topical skin preparation. [4] The topical skin preparation according to any one of [1] to [3], wherein the content of diamond nanoparticles is 1 mass% or less based on the total amount of the topical skin preparation. [5] The topical skin preparation according to any one of [1] to [4], wherein the content of platinum nanoparticles is 10 mass% or less based on the total amount of the topical skin preparation. [6] The topical skin preparation according to any one of [1] to [5], wherein the content of platinum nanoparticles is 1 mass% or less based on the total amount of the topical skin preparation. [7] The external skin preparation according to any one of [1] to [6], wherein the mass ratio of the content of platinum nanoparticles to the content of diamond nanoparticles is 0.01 to 100. [8] A fascia relaxant comprising the topical skin preparation according to any one of [1] to [7]. [9] An agent for improving negative mood states, comprising the topical skin preparation according to any one of [1] to [8].

[10] A body flexibility improver comprising the topical skin preparation according to any one of [1] to [9]. [Effects of the Invention]

[0007] According to the present invention, new uses for diamond nanoparticles and platinum nanoparticles are provided. [Brief explanation of the drawings]

[0008] [Figure 1] The results of imaging diagnosis in Test Example 1, where (a) is the thickness of the left lateral head of the gastrocnemius muscle of subject A before application of the topical skin preparation of Example 1, and (b) is the thickness of the left lateral head of the gastrocnemius muscle of subject A immediately after application of the topical skin preparation of Example 1. [Figure 2] The results of imaging diagnosis in Test Example 1, where (a) is the thickness of the left lateral head of the gastrocnemius muscle of subject B before application of the topical skin preparation of Example 1, and (b) is the thickness of the left lateral head of the gastrocnemius muscle of subject B immediately after application of the topical skin preparation of Example 1. DETAILED DESCRIPTION OF THE INVENTION

[0009] Hereinafter, embodiments of the present invention will be described, but the present invention is not limited to the following embodiments.

[0010] The external skin preparation according to this embodiment is based on the novel finding that it has the effect of relaxing fascia and muscles when applied to human skin.

[0011] Fascia is a collective term for the membranes of connective tissue, primarily collagenous, that exist beneath the skin and connect, stabilize, encase, and separate muscles and other internal organs. Muscle tissue covers all elements of the body in three dimensions, and abnormal adhesions between fascia impair the gliding properties and mobility of the fascia and all tissues beneath it, including the underlying muscle tissue, limiting the maintenance of antigravity posture and smooth, functional, and efficient movement. Furthermore, fascia has the crucial mechanical function of supporting and allowing the passage of blood vessels, nerves, and lymphatic vessels. Twisting of fascia affects not only muscle tissue but also blood vessels and nerves, and can even cause biochemical and immunological changes in the fascial membrane. These effects can result in dysfunctions throughout the body. Relaxing fascia to restore twisting improves mobility and extensibility between muscle tissues and between muscle tissue and other components, helping muscle tissue and other components function normally.

[0012] Fascia can become dysfunctional due to inflammation, pain, abnormal muscle tension, unbalanced muscle activity, abnormal posture, chronic physical or mental stress, etc. Dysfunction can cause fascial shortening, adhesions, changes in the density of the intercellular substance of the connective tissue, nutritional disorders in the area, limited range of joint motion, disuse atrophy and weakening of muscle tissue, decreased activity, poor alignment, poor circulation, abnormal tactile sensation, etc.

[0013] The topical skin preparation of this embodiment has the effect of relaxing fascia and untwisting it.As a result, it improves the flexibility of the body, such as improving the range of motion of muscle tissue and joints, and also suppresses cramps, muscle spasms, etc.The relaxation of fascia also reduces subjective tension, which makes it easier to feel fatigue recovery and can also suppress pain sensation.As a result, the topical skin preparation of this embodiment has the effect of improving negative mood states and can also improve vitality.

[0014] In this specification, "fascial relaxation" means to release abnormal adhesions and / or atrophy of the fascia and return the fascia to its normal state. "Fascia relaxation" is also called "fascial release."

[0015] Negative mood states in this specification include, among the moods felt by humans, feelings of anger, hostility toward others, feelings of confusion and embarrassment, depression, fatigue, lethargy, tension, anxiety, etc. Improving negative mood states means that these negative mood states are suppressed and become less likely to be felt.

[0016] In this specification, physical flexibility includes the ability of fascia and muscles to stretch, the range of motion of joints, etc., and improving physical flexibility means that fascia and muscles become more pliable and easier to stretch, the range of motion of joints increases, etc.

[0017] The topical skin preparation according to this embodiment contains at least diamond nanoparticles, platinum nanoparticles, and an organic sulfur compound.

[0018] The diamond nanoparticles according to this embodiment are nano-sized diamond particles with an average particle size of less than 1 μm. In this specification, the average particle size refers to the 50% volume average particle size (D50) (the same applies below). The average particle size is determined by dynamic light scattering (for example, a method of measuring using an electrophoretic light scattering photometer model ELS-8000).

[0019] As the diamond nanoparticles, for example, crude diamond (also called blended diamond or BD) synthesized by bombardment, and GD (Generated Diamond) obtained by refining BD can be used.

[0020] BDs produced by bombardment are composed of diamond and graphite particles with diameters ranging from several tens to several hundreds of nanometers. They are tightly aggregated aggregates of extremely small nanocluster-sized diamond units (diamond nanoparticles) with diameters ranging from 1.7 to 7 nm. In other words, they are tightly aggregated aggregates of at least four diamond nanoparticles, usually tens to hundreds, and sometimes thousands of diamond nanoparticles. BD particles are thought to have a core / shell structure in which the diamond surface is covered with graphite-based carbon. The graphite-based carbon surface contains numerous hydrophilic functional groups such as -COOH and -OH. This gives BDs excellent affinity for solvents containing -OH groups, such as water, alcohol, and ethylene glycol, and they disperse readily in these solvents. Water is the most suitable for BDs. BDs contain very small amounts of ultrafine (1.5 nm or less) amorphous diamond, graphite, and non-graphite carbon particles.

[0021] The impurities in BD can be divided into (i) water-soluble electrolytes (ionized), (ii) hydrolyzable groups and ionic materials (such as salts of functional surface groups) hydrogen-bonded to the diamond surface, (iii) water-insoluble materials (surface-attached impurities, insoluble salts, insoluble oxides), (iv) volatile materials, and (v) materials that are incorporated or encapsulated within the diamond crystal lattice.

[0022] (i) and (ii) are formed during the GD purification process. The water-soluble electrolyte (i) can be removed by washing with water, but treatment with an ion exchange resin is preferred for more effective removal. The water-insoluble impurities (iii) consist of separated microparticles such as metals, metal oxides, metal carbides, and metal salts (sulfates, silicates, carbonates), inseparable surface salts, and surface metal oxides. To remove these, it is preferable to convert them to a soluble form using an acid. The volatile impurities (iv) can usually be removed by heat treatment at 250 to 400°C in a vacuum of about 0.01 Pa.

[0023] For diamond nanoparticles, it is not necessary to completely remove the impurities from the BD, but it is preferable to remove 40 to 95% of the impurities (i) to (iii). The ratio of graphite to diamond can be adjusted by changing the conditions of the bombardment method and / or the purification conditions of the BD.

[0024] The GD is obtained as a dispersion of GD, which is produced by oxidizing a diamond-non-diamond mixture (initial BD) obtained by detonating an explosive, followed by neutralization with the addition of a basic material that is volatile or whose decomposition reaction products become volatile, and then separating the diamond-containing layer.

[0025] The oxidation treatment step comprises an oxidative decomposition treatment using nitric acid, followed by an oxidative etching treatment using nitric acid. The oxidative etching treatment comprises a first oxidative etching treatment and a second oxidative etching treatment, the first oxidative etching treatment being preferably carried out at a pressure and temperature higher than those of the oxidative decomposition treatment, and the second oxidative etching treatment being preferably carried out at a pressure and temperature higher than those of the first oxidative etching treatment. The oxidation treatment step is preferably carried out multiple times at 150°C to 250°C and 14 to 25 atmospheres for at least 10 to 30 minutes each.

[0026] After the oxidative etching process, a neutralization process is carried out to decompose and remove the nitric acid. The dispersion neutralized with a basic material is separated into a diamond-containing phase and a diamond-free phase by adding water and decanting.

[0027] After the diamond-containing phase is separated, the dispersion is further washed with nitric acid, and the lower phase dispersion containing the purified diamond particles is separated from the upper phase effluent. This separation is carried out by leaving the dispersion after the nitric acid washing treatment to stand.

[0028] The pH of the lower phase dispersion containing the purified diamond particles is preferably adjusted to 4 to 10, more preferably 5 to 8, and most preferably 6 to 7.5, and the diamond particle concentration is preferably adjusted to 0.05 to 16 mass %, more preferably 0.1 to 12 mass %, and most preferably 1 to 10 mass %.

[0029] The GD thus obtained has an elemental composition of 72-89.5% carbon, 0.8-1.5% hydrogen, 1.5-2.5% nitrogen, and 10.5-25% oxygen. Of the total carbon, 90-97% is diamond crystals, and 10-3% is non-diamond carbon. The average particle size (primary particles) is 2-50 nm. In the X-ray diffraction spectrum (XD) using Cu and Kα radiation as a radiation source, the Bragg angle (2Φ±0.2°) has the strongest peak at 43.9°, characteristic strong peaks at 73.5° and 95°, a strongly distributed halo at 17°, and essentially no peak at 26.5°. The specific surface area is 1.5×10 5 m 2 / kg or more, substantially all surface carbon atoms are bonded to heteroatoms, and the dispersion is 0.5m 3 The diamond particles have a total absorption space of 0.05 to 16 parts by mass, and the diamond particles have a total absorption space of 0.05 to 16 parts by mass. The particle size of the diamond particles is obtained by dynamic light scattering measurement using an electrophoretic light scattering photometer model ELS-8000.

[0030] The average particle size of the diamond nanoparticles is preferably 10 nm or more, 20 nm or more, or 100 nm or more, and is preferably 200 nm or less, and more preferably 100 to 200 nm.

[0031] The specific gravity of diamond nanoparticles is 2.50-3.45g / cm 3 It is preferable that the density is 2.63 to 3.38 g / cm 3 More preferably, it is 2.75 to 3.25 g / cm 3 The specific gravity of diamond nanoparticles is determined by the ratio of graphite to diamond, and the specific gravity of diamond is set to 3.50 g / cm. 3 , the specific gravity of graphite is 2.25g / cm 3 Calculating the ratio of diamond to graphite, the specific gravity is 2.63g / cm 3 corresponds to a composition of 30% diamond and 70% graphite by volume, with a specific gravity of 3.38 g / cm 3 corresponds to a composition of 90% diamond and 10% graphite by volume. Similarly, a specific gravity of 2.75 g / cm 3 is 40% diamond and 60% graphite by volume, with a specific gravity of 3.25g / cm 3 corresponds to a composition of 80% diamond and 20% graphite by volume. The specific gravity is 2.87 g / cm 3 corresponds to a composition of 50% diamond and 50% graphite by volume. The specific gravity is 2.63 g / cm 3 If it is less than 3.38 g / cm3, coloring due to graphite may become a problem. 3 Above this value, the far-infrared radiation effect is saturated, which is disadvantageous in terms of cost.

[0032] The content of diamond nanoparticles contained in the topical skin preparation is preferably 0.0001% by mass or more, 0.0003% by mass or more, 0.0005% by mass or more, or 0.0008% by mass or more, based on the total amount of the topical skin preparation, from the viewpoint of further improving the effect of relaxing fascia and muscles, and from the viewpoint of suppressing aggregation of diamond nanoparticles, it is preferably 10% by mass or less, 5% by mass or less, or 3% by mass or less, and from the viewpoint of suppressing blackening of the topical skin preparation, it is more preferably 1% by mass or less, 0.5% by mass or less, 0.3% by mass or less, or 0.1% by mass or less. The content of diamond nanoparticles contained in the topical skin preparation may be 0.0005 to 0.5% by mass, based on the total amount of the topical skin preparation.

[0033] The platinum nanoparticles according to this embodiment are nano-sized platinum particles, and refer to platinum particles with an average particle size of less than 1 μm.

[0034] Platinum nanoparticles can be obtained as platinum nanocolloids, for example, by the method described below.

[0035] That is, the method for producing platinum nanocolloids includes the steps of adding a peptide or glucosamine compound as a reducing agent, compound A represented by formula (1) or formula (2), to a solution in which a platinum-containing precious metal-containing compound has been dissolved, adding an alkali to assist the reducing properties of compound A, and forming precious metal microparticles (platinum microparticles) by a reduction reaction of the precious metal ions in the precious metal-containing compound. NR 4 R 5 -CR 2 R 3 -CO-R 1 (1) NR 4 R 5 -CR 6 R 7 -CR 2 R 3 -CO-R 1 (2)

[0036] where R 1represents hydrogen, a hydroxyl group, an alkoxy group, an amino group, or an atomic group bonded by a peptide bond, and R 2 and R 3 represents hydrogen, an alkyl group, or a substituted alkyl group, and R 4 and R 5 represent hydrogen, an alkyl group, a substituted alkyl group, or an acetyl group, and R 6 and R 7 respectively represent hydrogen, an alkyl group or a substituted alkyl group.

[0037] In the above production method, preferably, the solution is an aqueous solution. More preferably, in the step of adding alkali, the alkali is added so that the pH of the aqueous solution is 10 or higher. Even more preferably, in the step of forming precious metal fine particles, the precious metal fine particles are dispersed in an aqueous solution to form a precious metal colloid. Even more preferably, after the step of forming precious metal fine particles to form a precious metal colloid, the method further includes the steps of centrifuging the aqueous solution to separate it into a precipitate and a supernatant, and removing the supernatant to extract the precipitate.

[0038] In another preferred embodiment of the above production method, the solution is a solution of an organic solvent. More preferably, after the step of forming precious metal fine particles, the method further comprises the steps of allowing the solution to stand and separating it into a precipitate and a supernatant, and removing the supernatant to extract the precipitate. Even more preferably, after the step of removing the supernatant and extracting the precipitate, the method further comprises the step of adding water to the precipitate to form an aqueous precious metal colloid. Even more preferably, after the step of forming the precious metal colloid, the method further comprises the step of concentrating the precious metal colloid by ultrafiltration, ultracentrifugation, or the like. Still even more preferably, the method further comprises the step of adding a protective agent for organic solvents to the solution, and after the step of removing the supernatant and extracting the precipitate, the method further comprises the step of adding an organic solvent to the precipitate to form an organic solvent-based precious metal colloid.

[0039] The peptide compound A is a compound represented by the formula (1) or (2), 1 is an α- or β-amino acid with a hydroxyl group, R 1α- or β-amino acid compounds such as α- or β-amino acid esters in which R is an alkoxy group, α- or β-amino acid compounds in which the amino group is acetylated, 1 Examples include peptides in which the N-terminus is an α-amino acid, which is an atomic group connected by a peptide bond.

[0040] The glucosamine compound, Compound A, is a compound represented by the formula (1), 1 is hydrogen and R 2 is [-CH(OH)-CH(OH)-CH(OH)-CH 2 (OH) ] and R 3 , R 4 , R 5 Examples of glucosamine compounds include glucosamine, in which .alpha. is hydrogen, and its derivative N-acetylglucosamine.

[0041] The average particle size of the platinum nanoparticles is preferably 10 nm or more, 20 nm or more, or 100 nm or more, and is preferably 200 nm or less, and more preferably 20 to 200 nm.

[0042] The content of platinum nanoparticles contained in the topical skin preparation is preferably 0.000001% by mass or more, 0.000005% by mass or more, 0.00001% by mass or more, or 0.00005% by mass or more, based on the total amount of the topical skin preparation, from the viewpoint of further improving the effect of relaxing fascia and muscles; and preferably 10% by mass or less, 5% by mass or less, or 3% by mass or less, from the viewpoint of suppressing aggregation of the platinum nanoparticles; and more preferably 1% by mass or less, 0.5% by mass or less, 0.3% by mass or less, 0.1% by mass or less, 0.05% by mass or less, 0.01% by mass or less, 0.005% by mass or less, 0.001% by mass or less, 0.0005% by mass or less, or 0.0001% by mass or less, from the viewpoint of suppressing blackening of the topical skin preparation.

[0043] The mass ratio of the platinum nanoparticle content to the diamond nanoparticle content in the external skin preparation (platinum nanoparticle content / diamond nanoparticle content) is preferably 0.01 or more, 0.05 or more, or 0.1 or more, and is preferably 100 or less, 50 or less, or 10 or less. The mass ratio may be 0.01 to 100.

[0044] When an external skin preparation contains an organic sulfur compound together with diamond nanoparticles and platinum nanoparticles, it can achieve the effects of relaxing fascia and muscles, treating arthritis, and providing cosmetic effects. Examples of organic sulfur compounds include compounds having a sulfonyl group, a sulfonic acid group, a sulfonate group (a functional group in which the hydrogen atom of sulfonic acid is substituted with a metal atom, a halogen, or an organic group), a thiol group, a sulfide group, a tetrahydrothiophene ring, or a thiophene ring.

[0045] Examples of the organic sulfur compound having a sulfonyl group include methylsulfonylmethane, methylsulfonylethan, and ethylsulfonylethan.

[0046] Examples of organic sulfur compounds having a sulfonic acid group include azulene sulfonic acid, chondroitin sulfate, 2-aminoethanesulfonic acid (taurine), etc. Examples of organic sulfur compounds having a sulfonate group include salts of organic sulfur compounds having a sulfonic acid group, such as sodium azulene sulfonate, sodium chondroitin sulfate, and sodium 2-aminoethanesulfonate. The organic sulfur compounds having a sulfonic acid group or a sulfonate group may be compounds having a cyclic structure or may not have a cyclic structure.

[0047] Examples of organic sulfur compounds having a thiol group include L-cysteine, acetylcysteine, glutathione, pantetheine, etc. Examples of organic sulfur compounds having a sulfide group include methionine.

[0048] Examples of organic sulfur compounds having a tetrahydrothiophene ring include biotin, etc. Examples of organic sulfur compounds having a thiophene ring include thiamine, etc.

[0049] From the viewpoint of further improving the effect of relaxing fascia and muscles, the organic sulfur compound is preferably an organic sulfur compound having a sulfonyl group, and more preferably methylsulfonylmethane (DMSO2).

[0050] The molecular weight of the organosulfur compound is preferably 300 or less, 200 or less, 150 or less, or 100 or less, from the viewpoint of further improving the effect of relaxing fascia and muscles.

[0051] The content of the organic sulfur compound contained in the topical skin preparation is preferably 1% by mass or more, 3% by mass or more, or 5% by mass or more, based on the total amount of the topical skin preparation, in order to further improve the effect of relaxing the fascia and muscles, and is also preferably 20% by mass or less, 15% by mass or less, or 10% by mass or less.

[0052] The topical skin preparation according to this embodiment may be any of a pharmaceutical product, a quasi-drug product, and a cosmetic product. The form of these topical skin preparations is not particularly limited, and for example, topical skin preparations as pharmaceutical products or quasi-drugs may be in the form of a liquid, suspension, emulsion, gel, lotion, ointment, or sheet preparation in which a medicinal solution is impregnated into nonwoven fabric or the like. Furthermore, topical skin preparations as cosmetics may be, for example, skin care cosmetics such as lotion, emulsion, gel, cream, serum, lotion, or sheet mask, or makeup cosmetics such as foundation or lip balm. The topical skin preparation may be in the form of an O / W system, a W / O system, an aqueous system, an oil system, or an alcohol system.

[0053] When the topical skin preparation is an emulsion, the emulsion may be in the form of an O / W type, a W / O type, or a multi-layer emulsion. When the topical skin preparation is a cream, the cream may be in the form of an O / W type, a W / O type, or an anhydrous oil-based type. When the topical skin preparation is a gel, the gel may be in the form of an aqueous gel, an oil-based gel, or an oil gel. When the topical skin preparation is a serum, the serum may be in the form of a transparent or translucent lotion type, an O / W type, a W / O type, or a W / O / W type emulsion type, an oil type, or a two-liquid mixed type that is mixed at the time of use.

[0054] The topical skin preparation may contain water as a base or carrier commonly used in pharmaceuticals, quasi-drugs, or cosmetics. Examples of water include purified water, hot spring water, and deep sea water. Examples of bases or carriers other than water include oil-based bases.

[0055] Examples of oil-based bases include hydrocarbons such as squalane, liquid paraffin, petrolatum, solid paraffin, microcrystalline wax, and ceresin; oils and fats such as olive oil, almond oil, cacao oil, jojoba oil, macadamia nut oil, avocado oil, hardened palm oil, castor oil, sunflower oil, evening primrose oil, and synthetic triglycerides; waxes such as beeswax, lanolin, carnauba wax, and candelilla wax; higher alcohols such as cetyl alcohol, stearyl alcohol, behenyl alcohol, hexadecyl alcohol, octyldodecyl alcohol, and cholesterol; synthetic esters such as isopropyl myristate, glycerin triester, pentaerythritol tetraester, and cholesteryl ester; and silicone components such as dimethylpolysiloxane, methylphenylpolysiloxane, and cyclomethicone.

[0056] The topical skin preparation may contain other ingredients (additives) commonly used in pharmaceuticals, quasi-drugs, or cosmetics, such as surfactants, gelling agents, powders, alcohols, water-soluble polymers, film-forming agents, resins, inclusion compounds, antibacterial agents, fragrances, deodorants, salts, pH adjusters (pH buffers), freshening agents, plant-, animal-, or microbial-derived extracts, blood circulation promoters, astringents, antiseborrheic agents, moisturizers, emollients, solubilizers, thickeners, preservatives, emulsifiers, binders, chelating agents, keratolytic agents, enzymes, hormones, and vitamins.

[0057] The topical skin preparation may contain alcohols from the viewpoints of providing a cooling sensation, inhibiting bacterial growth, promoting dissolution of each component, etc. Known alcohols can be used, such as ethanol and isopropanol.

[0058] The topical skin preparation may contain a moisturizing agent from the viewpoint of protecting the stratum corneum, improving the feeling of use, etc. Known moisturizing agents can be used, and examples thereof include polyhydric alcohols such as glycerin, propylene glycol, dipropylene glycol, 1,3-butylene glycol, and polyethylene glycol; sugars such as hyaluronic acid, chondroitinic acid, maltitol, and sorbitol; and amino acids such as pyrrolidonecarboxylic acid and collagen.

[0059] The topical skin preparation may contain a softening agent from the viewpoint of improving skin emollience, moisturizing properties, feeling of use, etc. Known softening agents can be used, and examples of the softening agent include vegetable oils such as olive oil and jojoba oil; ester oils, squalane, and lanolin derivatives.

[0060] The topical skin preparation may contain a solubilizer to improve the dissolution of each component. Known solubilizers can be used, including surfactants such as polyoxin ethylene alcohol ether.

[0061] The topical skin preparation may contain a pH adjuster (pH buffer) to adjust the pH. Known pH adjusters (pH buffers) can be used, including citric acid, lactic acid, amino acids, and sodium citrate.

[0062] The topical skin preparation may contain a thickener from the viewpoint of improving the feeling during use, improving moisturizing properties, etc. Known thickeners can be used, and examples of such thickeners include alginates, cellulose derivatives, quince seed gum, pectin, pullulan, xanthan gum, gum arabic, Irish moss, tragacanth gum, pullulan, veegum, carboxyvinyl polymers, acrylic acid-based polymers, laponite, and cetanol.

[0063] The topical skin preparation may contain a fragrance to impart fragrance to the product. Known fragrances can be used, such as geraniol and linalool.

[0064] The topical skin preparation may contain alcohols from the viewpoints of providing a cooling sensation, inhibiting bacterial growth, promoting dissolution of each component, etc. Known alcohols can be used, such as ethanol and isopropanol.

[0065] The topical skin preparation may contain a preservative to inhibit the activity and proliferation of microorganisms. Known preservatives can be used, such as methylparaben and phenoxyethanol.

[0066] The topical skin preparation may contain an emulsifier to facilitate mixing of the aqueous and oil phase components. Known emulsifiers can be used, including nonionic surfactants such as glycerin monostearate, polyoxyethylene sorbitan fatty acid esters, sorbitan fatty acid esters, polyoxyethylene alkyl ethers, polyoxyethylene-polyoxypropylene block polymers, and polyoxyethylene hydrogenated castor oil esters; and anionic surfactants such as fatty acid soaps and sodium alkyl sulfates.

[0067] The topical skin preparation is used by being applied to the skin. Applying the topical skin preparation to the skin preferably means spreading it on the skin, and in the case of a sheet preparation or the like, it may be pasted on the skin. The topical skin preparation may be used by being applied to the skin when massaging muscles.

[0068] The site to which the external skin preparation is applied is not particularly limited, but is preferably joints such as knees, elbows, and shoulders, the waist, or the calves.

[0069] The amount and frequency of application of the topical skin preparation to the skin are not particularly limited, and can be determined appropriately depending on the age, weight, etc. of the subject.

[0070] By applying the topical skin preparation of this embodiment to the skin, it can relax the fascia, improve negative mood, or improve physical flexibility.Therefore, the present invention can be rephrased as a fascia relaxant, a negative mood improver, or a physical flexibility improver, which is composed of a topical skin preparation containing diamond nanoparticles, platinum nanoparticles, and an organic sulfur compound.The aspect of the topical skin preparation is as described above.

[0071] The present invention can also be understood as an invention relating to the following embodiments.

[0072] In one embodiment of the present invention, there is provided a method for relaxing fascia, which comprises applying to the skin an external skin preparation containing diamond nanoparticles, platinum nanoparticles, and an organosulfur compound.

[0073] Another embodiment of the present invention provides a method for improving negative mood states, which comprises applying to the skin an external skin preparation containing diamond nanoparticles, platinum nanoparticles, and an organosulfur compound.

[0074] Another embodiment of the present invention provides a method for improving bodily flexibility, which comprises applying to the skin an external skin preparation containing diamond nanoparticles, platinum nanoparticles, and an organic sulfur compound.

[0075] The external preparation for skin in each of the above embodiments is as described above. [Example]

[0076] The present invention will be described in more detail below with reference to examples and comparative examples, although the present invention is not limited to the following examples.

[0077] <Preparation of topical skin preparation> [Example 1] Based on the composition shown in Table 1, the mixture was stirred uniformly while heating using a stirring device, and then degassed and filtered to prepare an external skin preparation (gel).

[0078] The diamond colloid solution in the raw material is a dispersion of diamond nanoparticles in water, and the total content of methylsulfonylmethane, glycerin, 1,3-butylene glycol, cetanol, cetyl ethylhexanoate, ethanol, and glyceryl stearate in the raw material is 15 to 20 mass%.

[0079] [Table 1]

[0080] <Test Example 1: Imaging diagnosis of muscle relaxation using ultrasound examination> The topical skin preparation of Example 1 was applied to the calves of two adults (Subject A: male (55 years old), Subject B: male (57 years old)). Before and immediately after application of the topical skin preparation, the left lateral head of the gastrocnemius muscle was observed during contraction using an ultrasound examination device, and the thickness of the muscle was measured. Images of the observed left lateral head of the gastrocnemius muscle are shown in Figures 1 and 2. Figure 1 shows the observation results for Subject A, and Figure 2 shows the observation results for Subject B. (a) shows the state before application of the topical skin preparation of Example 1, and (b) shows the state immediately after application of the topical skin preparation of Example 1.

[0081] As shown in Figure 1, the thickness of the left gastrocnemius lateral head of subject A before application of the topical skin preparation of Example 1 was 19.5 mm, whereas the thickness of the left gastrocnemius lateral head after application of the topical skin preparation was 15.5 mm. Also, as shown in Figure 2, the thickness of the left gastrocnemius lateral head of subject B before application of the topical skin preparation of Example 1 was 15.2 mm, whereas the thickness of the left gastrocnemius lateral head after application of the topical skin preparation was 13.1 mm. Therefore, it can be seen that application of the topical skin preparation of Example 1 relaxed the fascia around the left gastrocnemius lateral head, reducing the muscle thickness during contraction.

[0082] [Subjective fatigue level (VAS)] The subjective sense of fatigue felt by the subjects was examined according to the method of the "Fatigue VAS (Visual Analogue Scale) Test" established by the Japan Fatigue Association.

[0083] A double-blind crossover study was conducted with 12 healthy female subjects aged 18 to 21 years. Specifically, the subjects chose either the topical skin preparation prepared in Example 1 or a placebo (a moisturizer was added instead of the diamond nanoparticles, platinum nanoparticles, and methylsulfonylmethane preparations in Example 1). The selected topical skin preparation was applied to the back and lower limbs daily after bathing at night for one week. Fatigue VAS scores were measured before and after the first application and before and after the final application one week later. This constituted one cycle, and after a two-week washout period, a new cycle was conducted using a different gel. The fatigue VAS scores are shown in Table 2.

[0084] [Table 2]

[0085] As a result of the above test, it was confirmed that after using the external skin preparation of Example 1, the subjects' sense of fatigue tended to decrease (p=0.078).

Claims

1. A topical skin preparation containing diamond nanoparticles, platinum nanoparticles, and an organic sulfur compound.

2. The topical skin preparation according to claim 1, wherein the organic sulfur compound is methylsulfonylmethane.

3. 3. The topical skin preparation according to claim 1, wherein the content of the diamond nanoparticles is 10 mass% or less based on the total amount of the topical skin preparation.

4. 3. The topical skin preparation according to claim 1, wherein the content of the diamond nanoparticles is 1 mass % or less based on the total amount of the topical skin preparation.

5. The topical skin preparation according to claim 1 or 2, wherein the content of the platinum nanoparticles is 10 mass% or less based on the total amount of the topical skin preparation.

6. The topical skin preparation according to claim 1 or 2, wherein the content of the platinum nanoparticles is 1 mass% or less based on the total amount of the topical skin preparation.

7. 3. The external skin preparation according to claim 1, wherein the mass ratio of the content of said platinum nanoparticles to the content of said diamond nanoparticles is 0.01 to 100.

8. A fascia relaxant comprising the topical skin preparation according to claim 1 or 2.

9. 3. An agent for improving negative mood states, comprising the topical skin preparation according to claim 1 or 2.

10. A body flexibility improving agent comprising the external skin preparation according to claim 1 or 2.

Images