Composition comprising ginseng seed oil

Abstract

To provide a stable emulsion composition in which a ginseng seed oil being an oily ingredient is uniformly mixed with a water-soluble ingredient.SOLUTION: The present disclosure relates to an emulsion composition containing a ginseng seed oil and a ginseng berry extract, where the composition is a single phase. Whereas a conventional ginseng seed oil-containing composition containing a water-soluble ingredient as a main ingredient has the problem in which formulation stability decreases as the ginseng seed oil being an oily ingredient is separated from the water-soluble ingredient, the emulsion composition according to the present disclosure exhibits superior stability, because the ginseng seed oil being the oily ingredient is uniformly mixed with the water-soluble ingredient and separation of the ginseng seed oil from the water-soluble ingredient is prevented for a long period of time.SELECTED DRAWING: Figure 1

Description

[Technical Field]

[0001] [CROSS-REFERENCE TO RELATED APPLICATIONS] This application claims priority to Korean Patent Application No. 10-2020-0039315, filed on March 31, 2020, the entire contents of which are incorporated herein by reference.

[0002] The present specification relates to an emulsified composition comprising ginseng seed oil and ginseng fruit extract. [Background technology]

[0003] Ginseng is a plant belonging to the genus Panax in the family Pentacarya. It has been cultivated in Korea, China, Japan, and other regions for over 2,000 years. Ginseng is a herbal medicine that has long been used for the purposes of disease prevention and longevity. Its known efficacy and effects include effects on the central nervous system, anti-carcinogenic and anti-cancer activities, immune function regulation, anti-diabetic effects, liver function enhancement, cardiovascular disorder improvement and anti-arteriosclerosis effects, blood pressure regulation, menopausal disorder improvement and osteoporosis effects, anti-stress and anti-fatigue effects, antioxidant activity, and anti-aging effects. Ginsenosides, the representative physiologically active components of ginseng, are widely distributed in the above-ground and underground parts of the plant, and considerable amounts are also found in ginseng seeds.

[0004] A great deal of research has been conducted on the main components and pharmacological effects of ginseng. Considering the pharmacological effects of ginseng, it is expected that long-term administration of ginseng can prevent various adult diseases and reduce the risk of developing them.

[0005] However, ginseng seed oil or a composition containing ginseng seed oil as a main ingredient is difficult to take due to its greasy nature, and must be taken in capsule form. Furthermore, when ginseng seed oil is contained in a composition containing a water-soluble component as a main ingredient, there is a problem that the ginseng seed oil, which is an oil component, separates from the water-soluble component, resulting in a decrease in product stability. [Prior art documents] [Patent documents]

[0006] [Patent Document 1] Korean Patent Publication No. 10-2014-0006538 Summary of the Invention [Problem to be solved by the invention]

[0007] In one aspect, the present invention aims to provide a stable emulsion composition in which carrot seed oil, an oil and fat component, is homogeneously mixed with a water-soluble component. [Means for solving the problem]

[0008] In one aspect, the present invention provides an emulsified composition comprising ginseng seed oil and ginseng fruit extract.

[0009] In an exemplary embodiment, the emulsion composition is a single phase.

[0010] In an exemplary embodiment, the ginseng may be selected from the group consisting of Korean ginseng (Panax ginseng), American ginseng (Panax quinquefolia), Sanshichi ginseng (Panax notoginseng), Vietnamese ginseng (Panax vietnamensis), Japanese ginseng (Panax japonicus), Panax elegatior, and Panax wangianus. It may contain one or more species selected from the group consisting of Panax bipinnatifidus and Himalayan ginseng.

[0011] In an exemplary embodiment, the ginseng seed extract may be an extract of water, C1-C6 alcohol, or a mixture thereof.

[0012] In an exemplary embodiment, the composition may contain 0.001 to 70 wt % of the ginseng seed oil based on the total weight of the composition.

[0013] In an exemplary embodiment, the composition may contain the ginseng seed extract in an amount of 0.01 to 60 wt % based on the total weight of the composition.

[0014] In an exemplary embodiment, the weight ratio of the ginseng seed oil and the ginseng fruit extract may be 1:2-100.

[0015] In an exemplary embodiment, the composition may further include gum and vegetable fiber.

[0016] In an exemplary embodiment, the gum may be xanthan gum, carrageenan gum, guar gum, gellan gum, locust bean gum, or the like. Bean gum, tamarind gum, gum arabic, konjac, pectin, agar, tapioca starch, dextrin, hydroxymethylcellulose (HPMC), sodium alginate, and modified starch.

[0017] In an exemplary embodiment, the plant fiber may include at least one selected from the group consisting of citrus plant fiber and wheat plant fiber. [Effects of the Invention]

[0018] In one aspect, the composition according to the present invention has excellent stability since the oil component, ginseng seed oil, is homogeneously mixed with the water-soluble component, and separation of the ginseng seed oil and the water-soluble component does not occur for a long period of time. [Brief explanation of the drawings]

[0019] [Figure 1] 1 shows a comparison of emulsion stability between Example 1 according to one aspect of the present disclosure and Comparative Example 1. [Figure 2] 1 shows a comparison of emulsion stability between Example 2 according to one aspect of the present disclosure and Comparative Example 2. [Figure 3] 1 is a graph showing the effect of ginseng seed oil and ginseng fruit extract according to one aspect of the present disclosure in promoting energy metabolism within muscle cells. [Figure 4] 1 shows the synergistic effect of ginseng seed oil and ginseng fruit extract on mitochondria production according to one embodiment of the present disclosure. DETAILED DESCRIPTION OF THE INVENTION

[0020] The present invention will be described in detail below.

[0021] In one aspect, the present disclosure provides an emulsion composition comprising ginseng seed oil and ginseng seed extract. By including ginseng seed oil and ginseng seed extract in a composition according to an embodiment of the present disclosure, separation of the ginseng seed oil, which is an oil component, from water-soluble components can be suppressed, thereby providing a stable emulsion composition.

[0022] In one embodiment, the emulsion composition may be a single-phase composition. As used herein, "single-phase" means that the oil phase and the water phase are not separated from each other and are maintained as a single phase in the emulsion composition.

[0023] The "oil phase" is a phase that is difficult to mix with the aqueous phase and may be a liquid oil, an organic solvent, or a mixture thereof at room temperature and atmospheric pressure. The "water phase" is a phase that is difficult to mix with the oil phase and may be any phase that can easily dissolve the water-soluble polymer, without particular limitations. For example, the water phase may include water, such as purified water or distilled water, polyol, etc.

[0024] In one embodiment, the ginseng (genus Panax) may include, but is not limited to, one or more selected from the group consisting of Korean ginseng (Panax ginseng), American ginseng (Panax quinquefolia), Sanshichi ginseng (Panax notoginseng), Vietnamese ginseng (Panax vietnamensis), Japanese ginseng (Panax japonicus), Panax elegatior (Panax wangianus), Uyo Sanshichi ginseng (Panax bipinnatifidus), and Himalayan ginseng (Panax pseudoginseng).

[0025] As used herein, methods for obtaining "carrot seed oil" include pressing, supercritical extraction, subcritical extraction, microwave extraction, ultrasonic extraction, and the like, but are not particularly limited as long as they are obvious to those skilled in the art. The carrot seeds used in producing carrot seed oil herein include split carrot seeds and germinated carrot seeds, such as carrots whose sprouts have begun to grow but have not yet sprouted. The carrot seeds may be raw carrot seeds or those dried by sun drying or hot air drying. For example, when using the pressing method, carrot seed oil may be obtained by placing carrot seeds or carrot seed powder in an oil press and pressing for 3 to 20 minutes at room temperature (about 15 to 25°C). Any oil press suitable for food products may be used. When using supercritical extraction, carrot seeds or carrot seed powder are placed in a supercritical extraction tank, 50 mL to 1 L of extraction solvent is added per 1 g of carrot seed powder, and then a supercritical fluid (e.g., carbon dioxide) is supplied. Extraction may be carried out for 3 to 4 hours while maintaining the pressure in the extraction tank at 300 to 500 bar.

[0026] In one embodiment, the ginseng seed oil may be purified as needed. The purification process is not particularly limited and may be any method known in the art. For example, the ginseng seed oil may be subjected to vacuum filtration, ion exchange resin or silica gel column chromatography, and then freeze-dried or concentrated to remove the solvent.

[0027] As used herein, "ginseng fruit" refers to the fruit obtained during the growth process of ginseng. Specifically, it refers to the fruit of 3- to 5-year-old ginseng, more specifically, the fruit that ripens for about a week in mid-July on 4-year-old ginseng. The ginseng fruit used herein is not limited by its method of acquisition; it may be cultivated or commercially available. Such ginseng fruit contains more vitamins, minerals, and ginsenosides than ginseng roots commonly used in the industry. In particular, the composition of ginsenosides contained in ginseng fruit is completely different from that of ginseng roots. More specifically, ginseng fruit contains more PT (protopanaxatriol) ginsenosides, including ginsenosides Re, Rg1, and Rg2, than PD (protopanaxadiol) ginsenosides, including ginsenosides Rb1, Rb2, Rc, and Rd, and thus exhibits far superior effects to ginseng roots.

[0028] In this specification, the term "ginseng seed extract" includes any substance obtained by extracting ginseng seed components, regardless of the extraction method, extraction solvent, extracted components, or extract form. The term "ginseng seed extract" is a broad concept that includes substances obtained by extraction methods that involve treatment with heat, acid, base, enzymes, etc. during the extraction process, as well as substances obtained by processing or treating the substances obtained by extracting ginseng seed components with other methods after extraction. Specifically, the processing or treatment may involve further fermentation or enzymatic treatment of the ginseng seed extract. Therefore, the ginseng seed extract referred to herein may be a fermented product. Furthermore, the ginseng seed extract referred to herein may be the ginseng seed extract described below, specifically an extract of fresh ginseng seeds or an extract of dried ginseng seeds. In the present specification, fresh ginseng seeds, dried ginseng seeds, or other processed ginseng seeds (e.g., fermented products, dried and pulverized products, etc.) that can be used for the extraction process may have compositions similar to or identical to each other. Therefore, it can be said that fresh ginseng seeds, dried ginseng seeds, or other processed ginseng seeds with similar or identical compositions exhibit the same effects and uses as the ginseng seed extract disclosed herein.

[0029] The extraction method for obtaining "ginseng seed extract" herein may include, but is not limited to, heat extraction, cold maceration extraction, reflux cooling extraction, ultrasonic extraction, or the like, as long as it is an extraction method obvious to those skilled in the art. The ginseng seeds used in the preparation of the ginseng seed extract herein may be dried ginseng seeds, fresh ginseng seeds, or ginseng seed powder, and any substance made from fresh ginseng seeds may be used without particular limitation. The ginseng seed extract herein may be an extract from water, an organic solvent, or a mixture thereof. Examples of the organic solvent include, but are not limited to, C1-C6 alcohols, acetone, hexane, ethyl acetate, carbon dioxide, or a mixture of two or more of these. The ginseng seed extract herein also includes an extract obtained by vacuum concentration of an extract obtained by extraction with the extraction solvent. The vacuum concentration may be performed at, but is not limited to, 35-50°C. Vacuum concentration at the above temperature range results in excellent synergistic effects between the various components in the ginseng seed extract of the present invention. From this viewpoint, the vacuum concentration may be carried out under conditions of, specifically, 36°C or higher, 37°C or higher, 38°C or higher, 39°C or higher, 40°C or higher, 41°C or higher, 42°C or higher, and / or 49°C or lower, 48°C or lower, 47°C or lower, 46°C or lower, 45°C or lower, 44°C or lower, or 43°C or lower. Specifically, the vacuum concentration may be carried out under conditions of 600 mmHg or more, 620 mmHg or more, 640 mmHg or more, 650 mmHg or more, 660 mmHg or more, 680 mmHg or more, 700 mmHg or more, 720 mmHg or more, 740 mmHg or more, and / or 760 mmHg or less, 740 mmHg or less, 720 mmHg or less, 700 mmHg or less, 680 mmHg or less, 660 mmHg or less, 650 mmHg or less, 640 mmHg or less, 620 mmHg or less, or 600 mmHg or less.

[0030] In one embodiment, the composition may contain 0.001 to 70 wt % of the ginseng seed oil based on the total weight of the composition. If the ginseng seed oil is contained in an amount exceeding 70 wt %, the stability of the composition may be reduced. Specifically, the composition contains the ginseng seed oil in an amount of 0.001% by weight or more, 0.002% by weight or more, 0.003% by weight or more, 0.005% by weight or more, 0.007% by weight or more, 0.01% by weight or more, 0.02% by weight or more, 0.03% by weight or more, 0.05% by weight or more, 0.07% by weight or more, 0.1% by weight or more, 0.2% by weight or more, 0.3% by weight or more, 0.5% by weight or more, 0.7% by weight or more, 1% by weight or more, 1.1% by weight or more, 1.2% by weight or more, 1.3% by weight or more, 1.4% by weight or more, 1.5% by weight or more, 2% by weight or more, 3% by weight or more, 4% by weight or more, 5% by weight or more, 6% by weight or more, 7% by weight or more, 8% by weight or more, 9% by weight or more, 10% by weight or more, 11% by weight or more, 12% by weight or more, 13% by weight or more, 14% by weight or more, 15% by weight or more, 16% by weight or more, 17% by weight or more, 18% by weight or more, 19% by weight or more, 20% by weight or more, 21% by weight or more, 22% by weight or more, 23% by weight or more, 24% by weight or more, 25% by weight or more, 26% by weight or more, 27% by weight or more, 28% by weight or more, 29% by weight or more, 30% by weight or more, 31% by weight or more, 32% by weight or more, weight% or more, 10 weight% or more, 11 weight% or more, 12 weight% or more, 13 weight% or more, 14 weight% or more, or 15 weight% or more and 70 weight% or less, 69 weight% or less, 68 weight% or less, 67 weight% or less, 66 weight% or less, 65 weight% or less, 64 weight% or less, 63 weight% or less, 62 weight% or less, 61 weight% or less, 60 weight% or less, 59 weight% or less, 58 weight% or less, 57 weight% or less, 56 weight% or less, 55 weight% or less, 54 weight% or less, 53 weight% or less, 52 weight% or less, 51 weight% or less, 50 weight% or less, 49 weight% or less, 48 ​​weight% or less, 47 weight% or less, 46 weight% or less, 45 weight% or less, 44 weight% or less , 43% by weight or less, 42% by weight or less, 41% by weight or less, or 40% by weight or less.

[0031] In one embodiment, the composition may contain the ginseng seed extract in an amount of 0.01 to 60 wt % based on the total weight of the composition. If the ginseng seed extract is contained in an amount exceeding 60 wt %, the fluidity may be poor, which may cause problems in the manufacturing process. Specifically, the composition may contain the ginseng seed extract in an amount of 0.01 wt % or more, 0.02 wt % or more, 0.03 wt % or more, 0.05 wt % or more, 0.07 wt % or more, 0.1 wt % or more, 0.2 wt % or more, 0.3 wt % or more, 0.5 wt % or more, 0.7 wt % or more, 1 wt % or more, 2 wt % or more, 3 wt % or more, 5 wt % or more, 7 wt % or more, 9 wt % or more, 10 wt % or more, 11 wt % or more, 12 wt % or more, 13 wt % or more, 14 wt % or more, or 15 wt % or more. The cellulose may comprise at least 15%, at least 16%, at least 17%, at least 18%, at least 19%, or at least 20% by weight, and at most 60%, at most 59%, at most 58%, at most 57%, at most 56%, at most 55%, at most 54%, at most 53%, at most 52%, at most 51%, at most 50%, at most 48%, at most 46%, at most 44%, at most 42%, or at most 40% by weight.

[0032] In one embodiment, the weight ratio of the ginseng seed oil to the ginseng seed extract may be 1:2 to 100, or 1:2 to 50, or 1:2 to 15, or 1:7 to 13, or 1: 10. If the weight ratio of the ginseng seed oil to the ginseng seed extract is outside the above range, the oil layer may separate, reducing the stability of the formulation.

[0033] In one embodiment, the composition does not contain a separate surfactant. The present disclosure provides an emulsion composition that is stably dispersed even when substantially free of surfactants. The term "substantially free of surfactants" means that the amount of surfactant in synthetic ingredients is minimized or may be completely absent, and refers to a content of 1% or less, preferably 0.5% or less, more preferably 0.1%, and even more preferably 0.01% or less, based on the total weight of the composition.

[0034] In one embodiment, the composition may further include gum and vegetable fiber.

[0035] In one embodiment, the gum may include one selected from the group consisting of xanthan gum, carrageenan gum, guar gum, gellan gum, locust bean gum, tamarind gum, gum arabic, konjac, pectin, agar, tapioca starch, dextrin, hydroxymethylcellulose (HPMC), sodium alginate, and modified starch.

[0036] In one embodiment, the composition may contain 0.001 to 20 wt% of the gum based on the total weight of the composition. If the gum is contained in an amount exceeding 5 wt%, excessive foam may be generated, resulting in an unpleasant feeling when drinking the composition. Specifically, the composition may contain the gum in an amount of 0.001 wt% or more, 0.002 wt% or more, 0.003 wt% or more, 0.005 wt% or more, 0.007 wt% or more, 0.01 wt% or more, 0.02 wt% or more, 0.03 wt% or more, 0.04 wt% or more, 0.05 wt% or more, 0.06 wt% or more, 0.07 wt% or more, 0.08 wt% or more, 0.09 wt% or more, or 0.1 wt%. or more, and 5% by weight or less, 4.9% by weight or less, 4.8% by weight or less, 4.7% by weight or less, 4.6% by weight or less, 4.5% by weight or less, 4.4% by weight or less, 4.3% by weight or less, 4.2% by weight or less, 4 .1% by weight or less, 4% by weight or less, 3.9% by weight or less, 3.8% by weight or less, 3.7% by weight or less, 3.6% by weight or less, 3.5% by weight or less, 3.4% by weight or less, 3.3% by weight or less, 3.2% by weight or less The cellulose may contain less than 3.1%, less than 3%, less than 2.9%, less than 2.8%, less than 2.7%, less than 2.6%, less than 2.5%, less than 2.4%, less than 2.3%, less than 2.2%, less than 2.1%, or less than 2% by weight.

[0037] In one embodiment, the plant fiber may include at least one selected from the group consisting of citrus plant fiber and wheat plant fiber.

[0038] In one embodiment, the composition may contain 0.001 to 20 wt% of the plant fiber based on the total weight of the composition. If the plant fiber is contained in an amount exceeding 5 wt%, excessive foaming may occur, resulting in reduced formulation stability. Specifically, the composition may contain the plant fiber in an amount of 0.001 wt% or more, 0.002 wt% or more, 0.003 wt% or more, 0.005 wt% or more, 0.007 wt% or more, 0.01 wt% or more, 0.02 wt% or more, 0.03 wt% or more, 0.04 wt% or more, 0.05 wt% or more, 0.06 wt% or more, 0.07 wt% or more, 0.08 wt% or more, 0.09 wt% or more, 0.1 wt% or more, 0.2 wt% or more, 0.3 wt% or more, 0.4 wt% or more, or 0.5 wt% or more, and 20 wt% or less, 19 wt% or less, or 18 wt% or less. , 17% by weight or less, 16% by weight or less, 15% by weight or less, 14% by weight or less, 13% by weight or less, 12% by weight or less, 11% by weight or less, 10% by weight or less, 9% by weight or less, 8% by weight or less, 7% by weight or less, 6% by weight or less, 5% by weight or less, 4.8% by weight or less, 4.6% by weight or less, 4.4% by weight or less, 4.2% by weight or less, 4% by weight or less, 3.8% by weight or less, 3.6% by weight or less, 3.4% by weight or less, 3.2% by weight or less, 3% by weight or less, 2.8% by weight or less, 2.6% by weight or less, 2.4% by weight or less, 2.2% by weight or less, or 2% by weight or less.

[0039] In one embodiment, the ginseng seed extract may be an extract of water, an organic solvent, or a mixture thereof. The water may include distilled water or purified water, and the organic solvent may be, but is not limited to, alcohol, acetone, hexane, ethyl acetate, carbon dioxide, or a mixture of two or more thereof. The alcohol may be a C1-C6 lower alcohol.

[0040] In one embodiment, the composition may be a liquid composition.

[0041] In one embodiment, the composition may be a food composition. The dosage form of the food composition is not particularly limited, and may be, for example, tablets, granules, pills, liquids such as drinks, caramels, gels, bars, tea bags, etc. In addition to the active ingredient, a person skilled in the art may easily select and blend ingredients commonly used in the art according to the dosage form or intended use in each dosage form of the food composition, and when used in combination with other ingredients, a synergistic effect may be produced.

[0042] The food composition according to one embodiment may contain various nutrients, vitamins, minerals (electrolytes), flavorings such as synthetic and natural flavorings, colorants and enhancers (e.g., for cheese, chocolate), pectinic acid and its salts, alginic acid and its salts, organic acids, protective colloid thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohol, and carbonation agents used in carbonated beverages. The food composition according to one embodiment may also contain natural fruit juice and fruit pulp for producing fruit juice beverages and vegetable beverages. These ingredients may be used independently or in combination. The proportion of these additives is not critical, but typically ranges from 0 to about 50 parts by weight per 100 parts by weight of the composition according to one embodiment.

[0043] In one embodiment, the composition may be a composition for promoting intramuscular energy metabolism.

[0044] In one embodiment, the composition may be a composition for promoting fat oxidation.

[0045] In one embodiment, the composition may be a composition for increasing and activating mitochondria.

[0046] In one aspect, the present disclosure provides a capsule containing the emulsion composition, which may be a soft or hard capsule.

[0047] In one aspect, the present disclosure provides use of ginseng seed oil and ginseng fruit extract for the manufacture of a composition for promoting intramuscular energy metabolism.

[0048] In one aspect, the present disclosure provides the use of ginseng seed oil and ginseng fruit extract for use in the manufacture of a composition for fat oxidation.

[0049] In one aspect, the present disclosure provides the use of ginseng seed oil and ginseng fruit extract for use in the manufacture of a composition for increasing and activating mitochondria.

[0050] The present invention will be described in more detail below with reference to examples, etc. However, it will be obvious to those skilled in the art that these examples are merely for the purpose of illustrating the present invention and that the scope of the present invention should not be construed as being limited by these examples.

[0051] [Production Example 1] Production of carrot seed oil Harvesting raw ginseng (Panax ginseng CA Meyer) fruits and seeds The seeds were separated to obtain ginseng seeds. The harvested ginseng seeds were washed to remove foreign matter, and only those suitable for pressing were selected. These were dried and crushed to remove impurities, and then placed in an oil press (DS-1500, Dasan Food Industry Machinery, Korea) and pressed to extract ginseng seed oil. The extracted ginseng seed oil was refined and filtered to produce ginseng seed oil (Korean ginseng seed oil).

[0052] [Production Example 2] Production of ginseng seed extract The pulp and juice of peeled fresh ginseng (Panax ginseng CA Meyer) were mixed to prepare ginseng seed raw material. 3 L of ethanol was added to 1 kg of the ginseng seed raw material, and the mixture was refluxed at 25°C for 4 hours and then concentrated under reduced pressure to prepare a ginseng seed extract (Korean ginseng seed extract).

[0053] [Production Example 3] Production of red ginseng extract 1 kg of raw red ginseng was placed in an extractor, followed by 3 L of a solvent made by mixing water and ethanol in a 1:1 volume ratio. Extraction was repeated four times at 70°C for 10 hours, and the extract was pressure-filtered using a 250 mesh filter. The liquid was transferred to a vacuum concentrator and concentrated at 600 mmHg and 70°C. It was then transferred to a sterilization tank and steam-sterilized at 83°C for 20 minutes to produce a red ginseng extract.

[0054] [Example 1] Example 1, an emulsion composition of ginseng seed oil and ginseng seed extract, was prepared according to the formulation in Table 1 below. Specifically, the ginseng seed oil of Preparation Example 1 was added to the ginseng seed extract of Preparation Example 2, and the mixture was heated to 70°C and stirred at 200 rpm using an aji mixer. A solution of the ginseng seed extract and ginseng seed oil was added to purified water, and the mixture was stirred at 80°C and 200 rpm using an aji mixer (or homogenizer) to prepare Example 1.

[0055] [Table 1]

[0056] [Example 2] Example 2, an emulsion composition of ginseng seed oil and ginseng seed extract containing gellan gum and plant fiber, was prepared according to the composition in Table 1. Specifically, the ginseng seed oil of Preparation Example 1 was added to the ginseng seed extract of Preparation Example 2, and the mixture was heated to 70°C and stirred at 200 rpm using an agitator mixer. Gellan gum and citrus plant fiber were added to purified water, and the mixture was heated to 80°C to hydrate the gellan gum and citrus plant fiber. The solution obtained by stirring the ginseng seed extract and ginseng seed oil was then added to the purified water in which the gellan gum and plant fiber had been hydrated, and the mixture was stirred at 80°C and 200 rpm using an agitator mixer to produce Example 2.

[0057] [Comparative Example 1] The composition of Comparative Example 1 was prepared according to the formulation in Table 1. Specifically, the ginseng seed oil of Preparation Example 1 was added to the red ginseng extract of Preparation Example 3, and the mixture was heated to 70°C and stirred at 200 rpm using an agitator. The solution of the red ginseng extract and ginseng seed oil was added to purified water, and the mixture was stirred at 80°C and 200 rpm using an agitator to prepare Comparative Example 1.

[0058] Comparative Example 2 A composition of Comparative Example 2 containing gellan gum and plant fiber was prepared according to the formulation in Table 1. Specifically, the ginseng seed oil of Preparation Example 1 was added to the red ginseng extract of Preparation Example 3, and the mixture was heated to 70°C and stirred at 200 rpm using an agitator mixer. Gellan gum and citrus plant fiber were added to purified water, and the mixture was heated to 80°C to hydrate the gellan gum and citrus plant fiber. The solution obtained by stirring the red ginseng extract and ginseng seed oil was then added to the purified water in which the gellan gum and plant fiber had been hydrated, and the mixture was stirred at 80°C and 200 rpm using an agitator mixer to prepare Comparative Example 2.

[0059] [Experimental Example 1] Confirmation of emulsion stability The mixing results were checked immediately after the production of Examples 1 and 2 and Comparative Examples 1 and 2, and after leaving them at 25°C for 24 hours, they were stirred again for 10 seconds to check whether or not the oil phase and the aqueous phase had separated. The results are shown in Table 2 and Figures 1 and 2.

[0060] [Table 2]

[0061] As can be seen from the results of Table 2 and Figures 1 and 2, in Examples 1 and 2 containing ginseng seed oil and ginseng fruit extract, the oil components were homogeneously mixed, and the homogeneity was stably maintained even after 24 hours. On the other hand, in Comparative Examples 1 and 2 containing ginseng seed oil and red ginseng extract, the oil component was not homogeneously mixed with the water-soluble component, resulting in separation of the oil phase, and in Comparative Example 2 containing gellan gum, a large amount of bubbles was generated.

[0062] [Experimental Example 2] Measurement of ginsenoside absorption To measure the amount of ginsenosides absorbed, Example 1, Comparative Example 1, ginseng seed extract (Preparation Example 2 diluted 4-fold with purified water), and red ginseng extract (Preparation Example 3 diluted 4-fold with purified water) were each passed through a human digestion simulation system, and the amount of ginsenosides transferred to Caco-2 cells (ATCC), which are human-derived small intestinal vesicles, was measured. Specifically, in the salivary phase of the human digestion simulation system, amylase (2 mL) was added to the samples (Example 1, Comparative Example 1, ginseng seed extract, red ginseng extract) and stirred at 37°C, pH 6.9, and 100 rpm for 3 minutes. In the gastric phase, pepsin (4 mL) was added to the samples that had passed through the salivary phase and then digested at 37°C, pH 2 (1 M HCl), and 100 rpm for 1 hour. Next, for the small intestinal phase, lipase, pancreatin, bile acid, and amyloglucosidase were added to the sample digested in the gastric phase, and then the mixture was stirred at 37°C, pH 5.3 (1M NaHCO3), and 100 rpm for 2 hours. The sample that had passed through the human digestion system was centrifuged, and the supernatant was collected. The supernatant was diluted 10-fold and treated with DMEM. Caco-2 cells were then cultured in a transwell using the treated DMEM at 37°C and 5% CO2 for 2 hours. The cultured Caco-2 cells were then harvested and analyzed for ginsenosides absorbed by the Caco-2 cells using high-performance liquid chromatography (HPLC). The amount of was measured and the results are shown in Table 3 below.

[0063] [Table 3]

[0064] As can be seen from the results in Table 3, Example 1 containing ginseng seed oil and ginseng fruit extract had the highest ginsenoside content absorbed by small intestinal cells and excellent dosage form stability.

[0065] [Experimental Example 3] Measurement of energy metabolism-related gene expression by adding carrot seed oil Mouse myoblasts (obtained from ATCC) were cultured in Dulbecco's Modified Essential Medium (DMEM; Sigma-Aldrich) supplemented with 10% fetal bovine serum (Hyclone) and 1% penicillin-streptomycin (P / S; Sigma-Aldrich) in a 5% CO2, 37°C incubator. When the plates were 100% confluent with myoblasts, they were serum starved (DMEM supplemented with 2% horse serum (Hyclone) and 1% P / S) for 1 week to generate myotubes. The differentiated myotubes were treated for 16 hours with ginseng seed oil (GSO) from Preparation Example 1, ginseng fruit extract (GB) from Preparation Example 2, and red ginseng extract (RG) from Preparation Example 3, either alone (100 μg / ml) or in combination (100 μg / ml for each sample, total 200 μg / ml for all samples). Fenofibrate (100 μM), a PARα agonist that promotes intramuscular energy metabolism, was used.

[0066] RNA was extracted from the treated myotubes using Trizol reagent (Invitrogen), and cDNA was synthesized using the RevertAid 1st strand cDNA synthesis kit (Fermentas). Q-PCR was performed using a Bio-Rad CFX96 thermal cycler. The expression levels of cyclophilin were used as a criterion for the expression of energy metabolism-related genes (Acyl-CoA Oxidase (ACO), Carnitine-palmitoyl transferase 1 (CPT1), Peroxisome Proliferator-activated Receptor α (PPARα), PPARγ-coactivator 1α (PGC1α), and transcription factor α (TFA). The relative expression levels of the mitochondrial proteins (tfam, uncoupling protein 3 (UCP3)) were compared and shown in Figure 3 (in Figure 3, GSO represents ginseng seed oil, GB represents ginseng fruit extract, RG represents red ginseng extract, and Fano represents fenofibrate).

[0067] As shown in Figure 3, ginseng seed extract showed higher expression levels of fat oxidation and mitochondria-related genes compared to red ginseng extract, confirming that ginseng seed oil had no significant effect on lipid metabolism within muscle cells. Furthermore, when the synergistic effects of red ginseng extract and ginseng seed extract with ginseng seed oil were confirmed, the addition of ginseng seed oil to red ginseng extract increased gene expression by approximately 20-30%, while the addition of ginseng seed oil to ginseng extract increased gene expression by approximately 30-50%. These results suggest that ginseng seed oil doubles the energy metabolism-promoting effects of red ginseng extract and ginseng seed extract, and that this synergistic effect is more pronounced with ginseng seed extract than with red ginseng extract. Furthermore, the increase in energy metabolism due to ginseng seed oil treatment was not observed in the fenofibrate-treated group, indicating that the synergistic effect of ginseng seed oil on energy metabolism promotion is specifically exhibited by red ginseng and ginseng seeds.

[0068] [Experimental Example 4] Observation of an increase in mitochondria in muscle cells by adding carrot seed oil Muscle cells differentiated using the same method and conditions as in Experimental Example 3 were treated with the sample and then washed twice with PBS to remove residual medium. The muscle cells were then treated with Mitotracker Green FM (Invitrogen) for 30 minutes to stain mitochondria. After washing with PBS three more times, the cells were fixed with formaldehyde (Sigma-Aldrich) and observed under a fluorescence microscope (EVOS M5000). The results are shown in Figure 4 (in Figure 4, GSO represents ginseng seed oil, GB represents ginseng fruit extract, RG represents red ginseng extract, and Fano represents fenofibrate).

[0069] As shown in FIG. 4, similar to the increase in expression of mitochondrial biogenesis-related genes (PGC1α, tfam) measured in Experimental Example 3, the increase in mitochondria due to the treatment with ginseng seed extract was observed. The results were significantly higher in the group treated with red ginseng extract than in the group treated with red ginseng extract. In particular, the group treated with both ginseng berry extract and ginseng seed oil showed an increase in mitochondrial density similar to the results of the positive control group (fenofibrate), suggesting that ginseng seed oil may actually promote energy metabolism within muscle cells.

[0070] [Dosage form example 1] Health drink Healthy drinks were prepared in a conventional manner according to the compositions shown in Table 4 below.

[0071] [Table 4]

[0072] The above ingredients were mixed using a normal health drink manufacturing method, and then stirred at 80°C for about 1 hour, after which the prepared solution was filtered and sterilized.

[0073] [Dosage Form Example 2] Soft capsule 50 mg of ginseng seed oil, 320 mg of ginseng fruit extract, 60 mg of L-carnitine, 2 mg of palm oil, 8 mg of hardened vegetable oil, 4 mg of yellow wax, and 6 mg of lecithin were mixed and filled into one capsule using a conventional method to prepare a soft capsule.

[0074] [Dosage Form Example 3] Liquid 2g of ginseng seed oil, 25g of ginseng fruit extract, 3g of isomerized sugar, 1g of mannitol, and 70ml of purified water were mixed using a conventional liquid preparation method, then filled into a brown mold and sterilized to produce a liquid preparation.

[0075] [Dosage Form Example 4] Drink 10g of carrot seed oil, 100g of carrot fruit extract, 10g of glucose, 0.6g of citric acid, and 25g of liquid oligosaccharides were mixed, and then 500ml of purified water was added and the mixture was bottled in 200ml portions. After filling into the bottles, the mixture was sterilized at 130°C for 4-5 seconds to produce a drinkable beverage.

Claims

1. An emulsified composition comprising Panax ginseng seed oil and Panax ginseng fruit extract.

2. 2. The emulsion composition according to claim 1, wherein the ginseng comprises at least one selected from the group consisting of Korean ginseng (Panax ginseng), American ginseng (Panax quinquefolia), Sanshichi ginseng (Panax notoginseng), Vietnamese ginseng (Panax vietnamensis), Japanese ginseng (Panax japonicus), Panax elegatior (Panax elegatior), Narrow-leaf ginseng (Panax wangianus), Uyo Sanshichi ginseng (Panax bipinnatifidus), and Himalayan ginseng (Panax pseudoginseng).

3. The ginseng seed extract contains water, C １ ~C ６ The emulsion composition according to claim 1 or 2, which is an extract of the above-mentioned alcohol or a mixed solvent thereof.

4. The emulsion composition according to any one of claims 1 to 3, wherein the composition contains the carrot seed oil in an amount of 0.001 to 70% by weight based on the total weight of the composition.

5. The emulsion composition according to any one of claims 1 to 4, wherein the composition contains the ginseng fruit extract in an amount of 0.01 to 60 wt% based on the total weight of the composition.

6. The emulsion composition according to any one of claims 1 to 5, wherein the weight ratio of the ginseng seed oil and the ginseng fruit extract is 1:2-100.

7. The emulsion composition according to any one of claims 1 to 6, further comprising a gum and a plant fiber.

8. 8. The emulsion composition according to claim 7, wherein the gum comprises at least one selected from the group consisting of xanthan gum, carrageenan gum, guar gum, gellan gum, locust bean gum, tamarind gum, gum arabic, konjac, pectin, agar, tapioca starch, dextrin, hydroxymethylcellulose (HPMC), sodium alginate, and modified starch.

9. The emulsion composition according to claim 7 , wherein the plant fiber comprises at least one selected from the group consisting of citrus plant fiber and wheat plant fiber.

10. The emulsion composition according to any one of claims 1 to 9, which is a liquid composition.

11. The emulsion composition according to any one of claims 1 to 10, wherein the composition is a food composition.

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