Hydroponic nutrient-enriched crops and method for growing the same

Abstract

SOLUTION: To provide a hydroponic nutrient-enriched crop; and to provide a method for growing the same, comprising: obtaining seeds to be grown, cultivating the seedlings, and obtaining the seedlings; preparing a basal growth solution containing nitrogen, phosphorus, and potassium, and cultivating the seedlings through basal hydroponic cultivation to obtain the hydroponic crops; and preparing an enriched growth solution with a higher trace element content than the basal growth solution, and cultivating the hydroponic crops through enriched hydroponic cultivation to obtain the hydroponic nutrient-enriched crops.EFFECT: By using a growth solution with a high trace element content to enhance crop growth, the types and concentrations of trace elements in hydroponic crops can be precisely increased, improving their nutritional value. This technology is suitable for cultivating different types of hydroponic crops, and can provide tailored nutritional solutions to meet the nutritional needs of different crops in different regions and by different people.SELECTED DRAWING: Figure 1

Description

[Technical Field]

[0001] The present application belongs to the field of agricultural technology, and in particular to hydroponic nutrient-enhanced crops and methods for growing them. [Background technology]

[0002] As a major food-producing country, China has always played an important role in ensuring the global food supply chain. However, with population growth, socio-economic development, and migration, land resources are becoming increasingly scarce. Therefore, how to maximize land resource utilization is one of the key development challenges. Traditional soil cultivation requires large areas of fertile soil, making it difficult to implement in large cities where food demand is huge and agricultural resources are scarce. Growing soil crops over long distances across provinces and even countries requires significant consumption of both transportation and energy.

[0003] In addition, in recent years, the intake of trace elements has become a health indicator of public concern. Daily intake of trace elements mainly comes from various types of food, but in the busy working environment, many people do not have enough time to manage their personal diet and consume sufficient trace elements. Although there are various types of nutritional supplements on the market, in addition to increasing the cost of living for the public, some consumers are more concerned that such products are not natural and may cause side effects on physical health, and are particularly cautious in selecting and purchasing nutritional supplements for the elderly, children, or pregnant women. Currently, the content of trace elements in crops is low, making it difficult to timely replenish nutritional components such as required specific trace elements with a single and small amount of crop.

[0004] Therefore, there is an urgent need to optimize the cultivation methods and nutritional components of crops, improve the cultivation production efficiency of crops, and enrich the nutritional value of crops. Summary of the Invention [Problem to be solved by the invention]

[0005] The purpose of this application is to provide hydroponic nutrient-enhanced crops and methods for growing them, which can partly solve the problem of optimizing the cultivation methods and nutrient components of crops. [Means for solving the problem]

[0006] In order to achieve the objectives of the above application, the technical solutions adopted in this application are as follows:

[0007] In a first aspect, the present application provides a method for producing a method of manufacturing a semiconductor device comprising: A step of obtaining seeds to be cultivated, cultivating the seeds, and obtaining the seeds; A step of preparing a basal growth solution containing nitrogen, phosphorus and potassium elements, and performing hydroponic basal growth on the seedling to obtain a hydroponic crop; The present invention provides a method for growing hydroponic crops that includes the steps of preparing an enriched growth solution having a higher trace element content than the trace element content in the basal growth solution, and performing hydroponic enrichment growth on the hydroponic crops to obtain the hydroponic enriched crops.

[0008] In some possible embodiments, the enriched growth solution contains nitrogen, phosphorus, and potassium elements, and also contains at least one trace element selected from the group consisting of calcium, iodine, iron, selenium, zinc, manganese, and copper.

[0009] In some possible embodiments, the content of each of the trace elements in the enriched growth solution is 2.5 μM or more.

[0010] In some possible embodiments, the conductivity of the fortified growth solution is 1 d / Sm-2 d / Sm.

[0011] In some possible embodiments, the pH value of the enriched growth solution is 5.5-6.5.

[0012] In some possible embodiments, the mass concentration of the nitrogen element in the enriched growth solution is 8 g / L-12 g / L, the mass concentration of the phosphorus element is 4 g / L-6 g / L, the mass concentration of the potassium element is 12 g / L-16 g / L, and the content of each of the trace elements is 2.5 μM-5 mM.

[0013] In some possible embodiments, the content of iodine element in the enriched growth solution is 2.5 μM-80 μM.

[0014] In some possible embodiments, the calcium element content in the enriched growth solution is 0.9 mM-5 mM.

[0015] In some possible embodiments, the content of iron element in the enriched growth solution is 0.25 mM-2 mM.

[0016] In some possible embodiments, the hydroponic intensive growth is carried out 7-10 days before the hydroponic culture is harvested.

[0017] In some possible embodiments, the environmental temperature for the hydroponic intensive growth is 20°C-23°C.

[0018] In some possible embodiments, the environmental relative humidity of the hydroponic enhanced growth is 50%-60%.

[0019] In some possible embodiments, the effective light dose in the hydroponic intensive growth stage is 200 μmol m -2 s -1 −250 μmol m -2 s -1 is.

[0020] In some possible embodiments, the photoperiod for the hydroponic intensive growth stage is 14-18 hours of light / 6-10 hours of darkness.

[0021] In some possible embodiments, the ratio of white light:blue light:red light during the hydroponic intensive growth stage is (1-1.5):1:(4-5).

[0022] In some possible embodiments, the molar ratio of the nitrogen element, the phosphorus element, and the potassium element in the basal growth solution is (8-12):(4-6):(12-16).

[0023] In some possible embodiments, the mass concentration of the nitrogen element in the basal growth solution is 8 g / L-12 g / L, the mass concentration of the phosphorus element is 4 g / L-6 g / L, and the mass concentration of the potassium element is 12 g / L-16 g / L.

[0024] In some possible embodiments, the basal growth solution further contains at least one element selected from the group consisting of calcium, magnesium, sulfur, iron, and manganese.

[0025] In some possible embodiments, the conductivity of the basal growth solution is 1 d / Sm-2 d / Sm.

[0026] In some possible embodiments, the pH value of the basal growth solution is 5.5-6.5.

[0027] In some possible embodiments, the environmental temperature for the hydroponic base growth is 20°C-23°C.

[0028] In some possible embodiments, the environmental relative humidity of the hydroponic base growth is 50%-60%.

[0029] In some possible embodiments, the effective light intensity in the hydroponic basal culture stage is 200 μmol m -2 s -1 −250 μmol m -2 s -1 is.

[0030] In some possible embodiments, the photoperiod during the hydroponic basal growth stage is 14-18 hours of light and 6-10 hours of darkness.

[0031] In some possible embodiments, the ratio of white light:blue light:red light in the hydroponic basal growth stage is (1-1.5):1:(4-5).

[0032] In some possible embodiments, the effective light intensity in the seedling development stage is 40 μmol m -2 s -1 −80 μmol m -2 s -1 is.

[0033] In some possible embodiments, the photoperiod during the seedling development stage is 10-14 hours of light / 10-14 hours of darkness.

[0034] In some possible embodiments, the ratio of blue light to red light during the seedling development stage is (1.5-2):1.

[0035] In some possible embodiments, the seeds are selected from vegetable seeds.

[0036] In some possible embodiments, the seedling cultivation is carried out by sterilizing the target seeds with alcohol, placing them in a cultivation cup containing a seedling sac, adding secondary distilled water to cover a protective film, and cultivating the seeds under an effective light intensity of 60 μmol m -2 s -1 and growing the seeds under conditions of a photoperiod of 12 hours light / 12 hours dark with a blue light:red light ratio of 1.7:1 until the seeds develop two leaflets, and then selecting the seeds for hydroponic cultivation.

[0037] In some possible embodiments, the hydroponic basal cultivation involves cultivating the seedlings in a hydroponic facility using a basal cultivation solution having a nitrogen, phosphorus, and potassium molar ratio of 10:5:14, at an environmental temperature of 20°C-23°C, a relative humidity of 50%-60%, and an effective light intensity of 230 μmol m -2s -1 The method includes growing the plants under conditions of a photoperiod of 16 hours of light and 8 hours of darkness with a white light:blue light:red light ratio of 1.2:1:4.5, regularly monitoring and maintaining the conductivity of the basal growth solution at 1 d / Sm-2 d / Sm and pH value at 5.5-6.5, and replacing the basal growth solution once a week.

[0038] In some possible embodiments, the hydroponic enrichment includes adding the trace elements to the basal growth solution to prepare an enriched growth solution having a content of each of the trace elements of 2.5 μM-5 mM; growing the hydroponic crops using the enriched growth solution 7-10 days before the hydroponic crops are harvested; and periodically detecting and replenishing the element types, element contents, pH value, and conductivity of the enriched growth solution.

[0039] In a second aspect, the present application provides a hydroponic, nutrient-enhanced crop grown by the above-described method for growing a hydroponic, nutrient-enhanced crop, wherein the content of trace elements in the hydroponic, nutrient-enhanced crop is higher than the content of trace elements in a hydroponic, basal-grown crop.

[0040] A first aspect of the present application relates to a method for growing hydroponic crops by cultivating seedlings using a basal culture solution. After the seedlings are obtained, hydroponic basal culture is performed using a basal culture solution. Then, hydroponic culture is performed using a culture solution with a higher trace element content than the basal culture solution. Growing crops using a hydroponic method saves the area required for soil cultivation and allows for precise control of cultivation conditions such as temperature, humidity, nutrients, and light exposure, creating a stable growth environment suitable for the crops, minimizing the impact of climatic conditions, and improving the production efficiency of crop cultivation. Growing crops using a culture solution with a high trace element content precisely enhances the type and content of trace elements in hydroponic crops, thereby improving the nutritional value of the hydroponic crops. This method is suitable for hydroponic cultivation of different types of hydroponic crops, and can provide targeted nutritional solutions by designing appropriate hydroponic crops to meet the nutritional needs of different regions and different populations.

[0041] According to a second aspect of the present invention, a hydroponic, nutritionally enhanced crop is obtained by growing it using the above-described method for growing a hydroponic, nutritionally enhanced crop, and the content of trace elements in the hydroponic, nutritionally enhanced crop is higher than the content of trace elements in a hydroponic, basic crop. By consuming this hydroponic, nutritionally enhanced crop, the human body can be better replenished with trace elements necessary for its function.

[0042] In order to more clearly explain the technical solutions in the embodiments of the present application, the following will briefly describe the drawings that need to be used in the description of the embodiments or prior art. The drawings in the following description are only some embodiments of the present application, and those skilled in the art can obtain other drawings based on these drawings without any creative work. [Brief explanation of the drawings]

[0043] [Figure 1] 1 is a flowchart of a method for growing hydroponic nutrient-enhanced crops according to an embodiment of the present application. [Figure 2] 1 is a photograph taken after completion of the cultivation of hydroponic Chinese cabbage according to Example 1 of the present application. [Figure 3] 1 shows the wet weight of hydroponic Chinese cabbages according to Examples 1 to 5 and Comparative Example 1 of the present application after completion of cultivation. [Figure 4] 1 shows the iodine content (iodine content / wet weight) of the hydroponic Chinese cabbages according to Examples 1 to 5 and Comparative Example 1 of the present application after completion of their growth. [Figure 5] 1 is a photograph of hydroponic butter lettuce after completion of cultivation according to Example 2 of the present application. [Figure 6] 1 shows the wet weight of hydroponic butter lettuce after completion of cultivation in Examples 2 to 10 and Comparative Example 2 of the present application. [Figure 7] 1 shows the calcium content (calcium content / wet weight) of hydroponic butter lettuce according to Examples 2 to 10 and Comparative Example 2 of the present application after completion of cultivation. [Figure 8] 1 shows the wet weights of hydroponic Chinese cabbages according to Examples 11 to 14 of the present application and Comparative Example 3 after completion of cultivation. [Figure 9]1 shows the iron content (iron content / wet weight) in hydroponic Chinese cabbages according to Examples 11 to 14 and Comparative Example 3 of the present application after completion of their growth. DETAILED DESCRIPTION OF THE INVENTION

[0044] In order to clarify the technical problems, technical solutions and beneficial effects of the present application, the present application will be described in more detail below in conjunction with examples. It should be understood that the specific examples described herein are only for the purpose of illustrating the present application and are not intended to limit the present application.

[0045] In this application, the term "and / or" describes a relationship between related objects and indicates that a three-way relationship may exist; for example, A and / or B may indicate that A exists alone, that A and B exist together, and that B exists alone. However, A and B may be singular or plural. The character " / " generally indicates that the related objects before and after it are in an "or" relationship.

[0046] As used herein, "at least one" refers to one or more, and "plurality" refers to two or more. "At least one of the following" or similar expressions refers to any combination of these terms, including any combination of single terms or multiple terms. For example, "at least one of a, b, or c" or "at least one of a, b, and c" can each refer to a, b, c, ab (i.e., a and b), ac, bc, or abc, where a, b, and c can each be one or more.

[0047] In various embodiments of the present application, the magnitude of the numbers of each of the above processes does not imply the order of execution, and some or all of the steps may be executed in parallel or one after the other, and it should be understood that the order of execution of each process should be determined by its function and internal logic, without limiting the implementation process of the embodiments of the present application.

[0048] The terms used in the examples of this application are merely for the purpose of describing particular examples and are not intended to limit the present application. As used in the examples of this application and the appended claims, the singular forms "a," "an," and "the" are also intended to include the plural forms unless the context clearly dictates otherwise.

[0049] The weights of relevant components described in the examples herein may indicate not only the specific content of each component but also the proportional weight relationship between each component, and therefore, even if the content of relevant components in the examples herein is scaled up or down proportionally, it will still be within the ranges disclosed in the examples herein. Specifically, the masses described in the examples herein may be in mass units known in the chemical industry, such as μg, mg, g, kg, etc.

[0050] The terms "first" and "second" are merely for the purpose of describing objects and distinguishing objects such as substances from one another, and should not be understood as indicating or implying relative importance or the number of technical features shown. For example, without departing from the scope of the embodiments of the present application, a first XX may be called a second XX, and similarly, a second XX may be called a first XX. Thus, a feature limited to "first" or "second" may explicitly or implicitly include one or more of the feature.

[0051] Traditional soil cultivation requires large areas of fertile soil, making it difficult to implement in large cities with huge food demands and limited agricultural resources. Furthermore, transporting soil-grown crops over long distances across provinces and even countries results in huge transportation and energy expenditures. To address these issues, the present study demonstrates that indoor hydroponics has emerged as a new cultivation technique, particularly suitable for vegetables as the main crop. By utilizing vertical space, indoor hydroponics significantly reduces the cultivation area required for soil cultivation, allowing production bases to be relocated from farmland to urban buildings with smaller floor space and higher building density. Indoor hydroponics can precisely control cultivation conditions such as temperature, humidity, nutrients, and light exposure using different equipment, creating an appropriate and stable growth environment for each crop. Furthermore, indoor hydroponics is not limited by climate, and crops traditionally grown in winter can be produced stably throughout the year using indoor hydroponics. Furthermore, indoor hydroponics generally has a faster cultivation cycle than soil cultivation, is less susceptible to natural disasters and insect damage, and does not require the use of pesticides. Hydroponic cultivation allows nutrients to be absorbed more evenly by the roots of crops, reducing the differences between individual plants, and allowing nutrients to be utilized, metabolized, and stored more effectively than traditional soil cultivation, promoting crop growth.

[0052] Indoor hydroponic cultivation technology can effectively and stably produce high-quality crops (e.g., vegetables). However, the initial investment required to build an indoor circulation-controlled hydroponic system and the high daily operating costs (especially energy costs) make the market price of hydroponic vegetables more expensive than traditional soil-grown vegetables. Furthermore, the trace element content of hydroponic crops is currently low, making it difficult to timely replenish necessary nutrients, such as specific trace elements, from a single crop in small quantities. As consumers increasingly focus on healthy eating, they are more willing to purchase high-quality, nutritious agricultural products at higher prices. However, they are less willing to purchase crops such as hydroponic vegetables, which are also expensive. As a result, hydroponic crops currently suffer from high costs and lack of market competitiveness.

[0053] The present invention aims to optimize the cultivation method of crops, improve the efficiency of crop cultivation, enrich the nutritional content of crops, and improve the nutritional value of crops. The present invention will be described in the following examples as an example of the technical solution of the present invention.

[0054] A first aspect of the present invention provides a method for growing hydroponic biofortified crops, which includes the following steps, as shown in FIG. 1 .

[0055] S10. Seeds to be cultivated are obtained, and seedlings are cultivated to obtain seedlings.

[0056] S20: Prepare a basal culture solution and perform hydroponic basal culture on the seedlings to obtain hydroponic crops. Here, the basal culture solution contains nitrogen, phosphorus, and potassium.

[0057] S30: Prepare an enriched growth solution and perform hydroponic enrichment on the hydroponic crop to obtain a hydroponic enriched crop, wherein the enriched growth solution has a higher trace element content than the basic growth solution.

[0058] A first embodiment of the present invention relates to a method for growing hydroponic crops. The method involves cultivating seeds, then cultivating them using a basal culture solution, followed by hydroponic basal cultivation using a culture solution with a higher trace element content than the basal culture solution. Growing crops using a hydroponic method reduces the amount of cultivation space required for soil cultivation and allows for precise control of cultivation conditions such as temperature, humidity, nutrients, and light exposure, creating a stable growth environment suited to each crop, minimizing the impact of climatic and environmental conditions, and improving the production efficiency of crop cultivation. Growing crops using a culture solution with a high trace element content improves the trace element content of hydroponic crops. By adjusting the type and content of trace elements in the culture solution, the type and content of trace elements in the hydroponic crops can be precisely adjusted, thereby improving the nutritional value of the hydroponic crops. This method is suitable for cultivating different types of hydroponic crops. Nutritionally enhanced hydroponic crops can be tailored to meet the nutritional needs of different regions and different populations, providing targeted nutritional solutions. The general public can obtain and replenish the nutrients such as trace elements required by the human body through their daily diet, and by eating daily ingredients, they can replenish trace elements that are natural and safe in a more convenient and economical way, which will comprehensively improve health and quality of life and has great prospects for agricultural application.

[0059] In step S10, the seeds to be cultivated are obtained, and the seedlings are cultivated. The seeds are provided with an appropriate growth environment under appropriate conditions such as temperature, humidity, light exposure, and nutrition, thereby promoting seed germination and growth, and the seedlings are obtained. The seeds in good condition are selected for subsequent hydroponic cultivation.

[0060] In some possible embodiments, the seeds are selected from vegetable seeds, such as Chinese cabbage seeds, lettuce seeds, celery, morning glory, cabbage, spinach, and Chinese broccoli. The seeds can be selected for later cultivation according to actual needs, which is highly adaptable, flexible, and convenient.

[0061] In some possible embodiments, the effective light intensity during seedling development is 40 μmol m -2 s -1 −80 μmol m -2 s -1 In the seeds according to the examples of the present application, the effective light intensity has a very important effect on the germination and growth of the seeds during the germination stage, and the seeds germinate better under conditions of such an effective light intensity, and light irradiation promotes the growth of the seedlings in the light during seed germination. For example, the effective light intensity during the seedling development stage is 40 μmol m -2 s -1 , 50 μmol m -2 s -1 , 60 μmol m -2 s -1 , 70 μmol m -2 s -1 , 80 μmol m -2 s -1 The value may be any typical but non-limiting point value such as or an interval value between any two point values.

[0062] In some possible embodiments, the photoperiod during the seedling development stage is 10-14 hours of light / 10-14 hours of darkness. Specifically, the seedlings are first exposed to 10-14 hours of light, followed by 10-14 hours of dark. This cycle is the length of the light and dark periods in the day-night cycle. In this case, an appropriate photoperiod can promote seed germination rate and speed. Photoperiods affect the synthesis and transport of plant hormones, which further affect seed germination and growth and development. Daylight activates a series of signaling pathways and promotes the synthesis and transport of auxin, which contributes to post-germination growth stages of seeds, such as root elongation and differentiation. Long-day irradiation also promotes chlorophyll synthesis and photosynthesis, allowing crops to obtain sufficient energy and nutrients, further promoting seed germination and growth. Illustratively, the photoperiod in the seedling development stage may be any typical but non-limiting point value such as 12 hours of light / 12 hours of darkness, 10 hours of light / 10 hours of darkness, 11 hours of light / 11 hours of darkness, 13 hours of light / 13 hours of darkness, 14 hours of light / 14 hours of darkness, 12 hours of light / 10 hours of darkness, 14 hours of light / 12 hours of darkness, or 13 hours of light / 11 hours of darkness, or an interval value between any two point values.

[0063] In some possible embodiments, the ratio of blue light to red light during the seedling development stage is (1.5-2):1. With this blue light to red light ratio, the seeds of the examples of the present application absorb a large amount of blue light during the seed germination stage, which has a positive effect on seed germination and seedling growth, and can also promote the growth of crop seedlings. Specifically, blue light has a significant effect on seed photosynthesis and photogenesis, promoting chlorophyll synthesis and activating photosynthetic enzyme activity, thereby increasing crop photosynthetic efficiency and growth rate and increasing stem and culm length. Furthermore, blue light improves seedling root system vitality, total absorption area, and active absorption area, which is beneficial for the growth and development of crop root systems. Red light can also stimulate seed metabolic activity and accelerate seed germination. Red light improves seed germination rate by adjusting the balance of plant hormones, for example, by increasing gibberellin (GA) content and decreasing abscisic acid (ABA) levels. Red light can also increase chlorophyll synthesis, improve photosynthetic efficiency, provide more energy and nutrients to seedlings, promote seedling growth, and improve crop stress tolerance.

[0064] In some possible embodiments, seedling cultivation involves sterilizing the target seeds with alcohol, placing them in a cultivation cup containing a seedling sac, adding secondary distilled water, covering them with a protective film to maintain humidity, and providing an effective light intensity of 60 μmol m -2 s -1 The method includes a step of growing the seeds until they have two leaflets under conditions of a photoperiod of 12 hours of light / 12 hours of darkness with a blue light:red light ratio of 1.7:1, and then selecting the seeds for hydroponic cultivation. Specific humidity and temperature conditions may also be established as needed. Seeds grown under these conditions of effective light intensity, photoperiod, and red / blue light ratio have a better germination rate and are in good condition.

[0065] In step S20, a basal culture solution is prepared, and the seedlings are subjected to hydroponic basal culture using the basal culture solution to grow into hydroponic crops, where the basal culture solution contains nitrogen, phosphorus, and potassium. Nitrogen, phosphorus, and potassium are three nutrient elements that are extremely important for crop growth. Of these, nitrogen mainly promotes chlorophyll synthesis and protein production in crops and is particularly important during the vegetative growth stage of crops. Phosphorus is important for root development and nutrient absorption, contributing to cell division and root system development in crops. Potassium enhances the disease resistance and water use efficiency of crops and is particularly important during the flowering and fruit ripening stages of crops.

[0066] In some possible embodiments, the molar ratio of nitrogen, phosphorus, and potassium in the basal growth solution is (8-12):(4-6):(12-16). Illustratively, the molar ratio of nitrogen, phosphorus, and potassium in the basal growth solution may be any typical, non-limiting value, such as 8:4:12, 8:5:13, 8:6:16, 10:5:14, 10:6:16, 12:4:12, 12:6:16, or any interval value between any two of the typical, non-limiting values.

[0067] In some possible embodiments, the mass concentration of nitrogen in the basal growth solution is 8 g / L-12 g / L, the mass concentration of phosphorus is 4 g / L-6 g / L, and the mass concentration of potassium is 12 g / L-16 g / L. For example, the mass concentration of nitrogen in the basal growth solution may be any typical, non-limiting point value such as 8 g / L, 9 g / L, 10 g / L, 11 g / L, or 12 g / L, or an interval value between any two point values, the mass concentration of phosphorus may be any typical, non-limiting point value such as 4 g / L, 5 g / L, or 6 g / L, or an interval value between any two point values, and the mass concentration of potassium may be any typical, non-limiting point value such as 12 g / L, 13 g / L, 14 g / L, 15 g / L, or 16 g / L, or an interval value between any two point values.

[0068] In the above examples, the present application demonstrates better hydroponic growth of seeds in the basal nutrient solution. Specifically, nitrogen is an important component of chlorophyll and is also a component of many enzymes in crops. When nitrogen is adequately supplied in the nutrient solution, crops undergo normal photosynthesis, resulting in vigorous growth, high fruit set, and high yields. A lack of nitrogen in the nutrient solution inhibits chlorophyll formation, causing leaves to pale and yellow, weakening or even halting photosynthesis, hindering the formation of new cells, and stunting crop growth and development. However, too much nitrogen consumes too much of the carbohydrates synthesized by the crop, converting them into proteins and other substances, which hinders the synthesis of cellulose and other substances, potentially causing crop lodging. Phosphorus is an important component of cell membranes and plays an important role in the energy transmission of crops. Phosphorus is also an important element in crop photosynthesis, allowing crops to convert solar energy into food, fiber, and oil. A lack of phosphorus in the nutrient solution results in slower crop growth, lower yields, poorer quality, and water shortages. The element potassium contributes to the regulation of water pressure in crop cells and strengthens crop stems, allowing them to better adapt to drought, floods and temperature fluctuations, maximizing crop yield. Potassium deficiency causes reduced crop growth rates, delayed pollination and maturation, stunted leaf development, reduced crop yield, weakened stems and culms, and water shortages.

[0069] In some embodiments, the nitrogen element in the growing solution may be provided by ammonia nitrogen, nitrates, etc., the phosphorus element may be provided by phosphates, and the potassium element may be composed of soluble potassium, such as ammonium molybdate, ammonium nitrate, calcium nitrate, potassium borate, potassium nitrate, potassium phosphate, etc.

[0070] In some possible embodiments, the basal growth solution further contains at least one element selected from the group consisting of calcium, magnesium, sulfur, iron, and manganese. In this case, all of these elements added to the basal growth solution contribute to promoting crop growth. Calcium is an important component of crop cell walls and plays an important role in maintaining cell wall stability and integrity. Magnesium is a component of chlorophyll and plays an important role in crop photosynthesis. Sulfur is an important component of proteins and has a significant impact on crop growth, development, and quality. Iron is a cofactor for many enzymes involved in crop chlorophyll synthesis and respiration. Manganese is an activator of various enzymes in crops and plays an important role in crop growth, development, and metabolism. In some examples, the basal growth solution further contains elements such as calcium, magnesium, sulfur, iron, and manganese, which may be provided in the form of nitrates, chlorides, oxides, phosphates, and the like.

[0071] In some possible embodiments, the conductivity of the basal growing solution is 1 d / Sm-2 d / Sm. In the examples of the present application, the conductivity affects the concentration of the growing solution, reflecting the content of soluble salts in the growing solution and related to the ease with which the root system of a plant absorbs water and nutrients from the growing solution. This conductivity condition contributes to improved plant growth and development efficiency and improved plant quality. However, a high-conductivity growing solution contains a large amount of soluble salts, which can cause osmotic stress because the osmotic pressure of the growing solution is higher than the osmotic pressure within the plant cells, making it difficult for the plant root system to absorb water. Osmotic stress can affect the normal growth and development of the plant, causing problems such as leaf curling, growth restriction, and reduced yield. For example, the conductivity of the basal growing solution can be any typical but non-limiting value, such as 1 d / Sm, 1.5 d / Sm, or 2 d / Sm, or an interval value between any two values.

[0072] In some possible embodiments, the pH value of the basal growing solution is 5.5-6.5. At this pH value, the nutrients in the growing solution are stable, contributing to the promotion of crop growth. However, if the pH value is too high, elements such as iron, manganese, copper, and zinc precipitate, preventing the crop from absorbing these important nutrients. This can slow crop growth and cause leaf yellowing and nutrient deficiency symptoms. Conversely, if the pH value is too low, the crop may absorb too many elements, such as aluminum and manganese, resulting in crop toxicity, manifested as root yellowing and necrosis. For example, the pH value of the basal growing solution may be any typical but non-limiting value, such as 5.5, 6, or 6.5, or any interval between any two values.

[0073] In some embodiments, the conductivity and pH value of the basal growth solution can be adjusted with 1 M potassium hydroxide and 5% dilute phosphoric acid.

[0074] In some possible embodiments, the environmental temperature for hydroponic base cultivation is 20° C.-23° C. Under such temperature conditions, the growth rate of the crop can be increased, the health of the root system of the crop can be improved, and the yield and quality of the crop can be ensured. Illustratively, the environmental temperature for hydroponic base cultivation may be any point value, such as 20° C., 21° C., 22° C., or 23° C., but is not limited thereto, or an interval value between any two points.

[0075] In some possible embodiments, the environmental relative humidity for hydroponic base cultivation is 50%-60%. Such humidity conditions can suppress the growth of pathogens, prevent infection of the crop root system, and prevent dehydration and wilting of the crop. This is more beneficial for crop photosynthesis and promotes crop growth and development. For example, the environmental relative humidity for hydroponic base cultivation may be any typical but non-limiting value, such as 50%, 52%, 54%, 55%, 56%, 58%, or 60%, or an interval value between any two values.

[0076] In some possible embodiments, the effective light intensity during the hydroponic basal growth stage is 200 μmol m -2 s-1 −250 μmol m -2 s -1 The effective light condition provides sufficient light irradiation for crop growth, which is beneficial for crop photosynthesis, and sufficient energy accumulation is beneficial for crop growth. For example, the effective light intensity in the hydroponic basic growth stage is 200 μmol m -2 s -1 , 210 μmol m -2 s -1 , 220 μmol m -2 s -1 , 230 μmol m -2 s -1 , 240 μmol m -2 s -1 , 250 μmol m -2 s -1 The value may be any point value or an interval value between any two point values, as typical but non-limiting examples.

[0077] In some possible embodiments, the photoperiod for the hydroponic basal growth stage is 14-18 hours of light and 6-10 hours of darkness. This photoperiod condition provides sufficient photosynthesis for crop growth, storing enough energy to promote crop growth. Exemplarily, the photoperiod for the hydroponic basal growth stage may be any point value or interval value between any two point values, such as, but not limited to, 14 hours of light and 6 hours of darkness, 15 hours of light and 8 hours of darkness, 16 hours of light and 10 hours of darkness, 16 hours of light and 8 hours of darkness, 15 hours of light and 7 hours of darkness, or 14 hours of light and 9 hours of darkness.

[0078] In some possible embodiments, the ratio of white light:blue light:red light in the hydroponic basal growth stage is (1-1.5):1:(4-5). In such a hydroponic growth process, red light and blue light are particularly important for crop photosynthesis, as they match the absorption peaks of chlorophyll. Red light contributes to crop flowering and fruit development, while blue light is important for crop chlorophyll formation and leaf development in the early stages of growth. White light is close to natural light and is beneficial for crop growth and development. For example, the ratio of white light:blue light:red light in the hydroponic basal growth stage may be any typical but non-limiting value, such as 1.2:1:4.5, 1:1:4, 1:1:5, 1.2:1:5, 1.4:1:4, 1.4:1:4.5, 1.4:1:5, 1.5:1:4, 1.5:1:4.5, or 1.5:1:5, or any interval value between any two of these values.

[0079] In some possible embodiments, the hydroponic basal culture is performed by cultivating seedlings in a hydroponic facility using a basal culture solution with a nitrogen, phosphorus, and potassium molar ratio of 10:5:14, at an environmental temperature of 20°C-23°C, a relative humidity of 50%-60%, and an effective light intensity of 230 μmol m -2 s -1 The photoperiod is 16 hours light / 8 hours dark with a white light:blue light:red light ratio of 1.2:1:4.5, the conductivity of the basal growth solution is regularly monitored and maintained at 1 d / Sm-2 d / Sm and a pH value of 5.5-6.5, and the basal growth solution is replaced once a week. These hydroponic basal growth conditions are more favorable for the growth of seeds and seedlings and improve crop yield and quality.

[0080] The above step S30 is as follows.

[0081] In some possible embodiments, the enriched growing solution contains nitrogen, phosphorus, and potassium, as well as at least one trace element selected from the group consisting of calcium, iodine, iron, selenium, zinc, manganese, and copper. In this case, in addition to the basic elements of nitrogen, phosphorus, and potassium, trace elements such as calcium, iodine, iron, selenium, zinc, manganese, and copper are added to the growing solution, allowing for enrichment of different types of trace elements to precisely improve the nutritional value of hydroponic crops. Depending on the nutritional needs of different regions or different populations, appropriate enriched hydroponic crops can be designed to provide targeted nutrient supplementation solutions.

[0082] In some embodiments, trace elements such as calcium, iodine, iron, selenium, zinc, manganese, and copper in the enriched growth solution can be provided in the form of nitrates, chlorides, oxides, phosphates, etc., nitrogen can be provided as ammonia nitrogen, nitrates, etc., phosphorus can be provided as phosphates, and potassium can be composed of soluble potassium, such as ammonium molybdate, ammonium nitrate, calcium nitrate, potassium borate, potassium nitrate, potassium phosphate, etc.

[0083] In some possible embodiments, the content of each trace element in the enriched growth solution is 2.5 μM or more. In some possible embodiments, the content of each trace element in the enriched growth solution is 2.5 μM-5 mM. In the above example, the content of trace elements in the enriched growth solution can sufficiently ensure the content of trace elements in hydroponic crops, allowing users to provide nutritionally enriched crops.

[0084] In some possible embodiments, the mass concentration of nitrogen in the enriched growth solution is 8 g / L-12 g / L, the mass concentration of phosphorus is 4 g / L-6 g / L, and the mass concentration of potassium is 12 g / L-16 g / L, and the contents of various trace elements are 2.5 μM-5 mM. In this case, nitrogen, phosphorus, and potassium in the enriched growth solution are three basic nutrients required for crop growth, and their contents are sufficient to ensure normal growth and development of the crop. The specific concentrations of trace elements allow the crop to absorb specific types and contents of trace elements during growth, resulting in hydroponic crops with enriched contents of specific trace elements.

[0085] For example, in the fortified growth solution, the mass concentration of nitrogen element may be any point value, such as 8 g / L, 9 g / L, 10 g / L, 11 g / L, 12 g / L, or an interval value between any two point values, which are typical but not limited to these values; the mass concentration of phosphorus element may be any point value, such as 4 g / L, 5 g / L, 6 g / L, or an interval value between any two point values, which are typical but not limited to these values; and the mass concentration of potassium element may be any point value, such as 12 g / L, 13 g / L, 14 g / L, 15 g / L, or an interval value between any two point values, which are typical but not limited to these values. , 16g / L, etc., which are typical but not limited to, and the content of each trace element may be any point value or an interval value between any two point values, which are typical but not limited to, 2.5μM, 10μM, 20μM, 50μM, 100μM, 200μM, 500μM, 800μM, 1000μM, 1.2mM, 1.5mM, 2mM, 2.5mM, 3mM, 3.5mM, 4mM, 4.5mM, 5mM, etc.

[0086] In some embodiments, the iodine content of the enriched growth solution is 2.5 μM-80 μM. In this case, hydroponic enrichment can significantly improve the iodine content of crops and better provide consumers with abundant iodine. For example, the iodine content of the enriched growth solution can be any typical but non-limiting value, such as 2.5 μM, 10 μM, 20 μM, 30 μM, 40 μM, 50 μM, 60 μM, 70 μM, or 80 μM, or an interval value between any two values.

[0087] In some embodiments, the calcium content of the enriched growth solution is 0.9 mM-5 mM. In this case, hydroponic enrichment can significantly improve the calcium trace element content of crops and better provide consumers with abundant calcium trace elements. For example, the calcium content of the enriched growth solution may be any typical but non-limiting value, such as 0.9 mM, 1.8 mM, 2.7 mM, 3.6 mM, 4.5 mM, or 5 mM, or an interval value between any two values.

[0088] In some embodiments, the iron content of the enriched growth solution is 0.25 mM-2 mM. In this case, hydroponic enrichment can significantly improve the iron content of crops and better provide consumers with abundant iron trace elements. For example, the iron content of the enriched growth solution can be any typical but non-limiting value, such as 0.25 mM, 0.5 mM, 0.75 mM, 1 mM, or 2 mM, or an interval value between any two values.

[0089] In some embodiments, the nutritional supplements can be prepared into powder, which is convenient for storage and application. According to the actual application needs, the nutritional supplements with specific contents and characteristics of trace elements can be accurately added to the basal growth solution.

[0090] In some possible embodiments, hydroponic crops are grown 7 to 10 days before harvest. In this case, the 7 to 10 days before harvest are intended for growth maturity. Performing hydroponic growth at this time is beneficial for ensuring further growth of the crops and for ensuring the absorption and fixation of specific trace elements in the growth solution by the crops. This avoids the influence of enriched trace elements on later growth of the crops. For example, hydroponic growth is performed at any point, such as 7 days, 8 days, 9 days, or 10 days before harvest, which are typical but non-limiting examples, or at an interval between any two points.

[0091] In some possible embodiments, the conductivity of the enriched growing solution is 1 d / Sm-2 d / Sm. Conductivity is an index for measuring the ion concentration in a solution and reflects the strength of trace elements in the growing solution. This conductivity more effectively promotes the absorption of trace elements into hydroponic plants. For example, the conductivity of the enriched growing solution may be any point value, such as 1 d / Sm, 1.5 d / Sm, or 2 d / Sm, which are typical but not limited to values, or an interval value between any two point values.

[0092] In some possible embodiments, the pH value of the enriched growing solution is 5.5-6.5. The pH value affects the solubility of trace elements and the absorption ability of hydroponic crop roots. Such pH value conditions contribute to hydroponic crops more effectively absorbing trace elements in the growing solution. Exemplarily, the pH value of the enriched growing solution may be any typical but non-limiting point value such as 5.5, 6, or 6.5, or an interval value between any two point values.

[0093] In some embodiments, the conductivity and pH value of the fortified growth solution can be adjusted with 1 M potassium hydroxide and 5% dilute phosphoric acid.

[0094] In some possible embodiments, the environmental temperature for hydroponic intensive cultivation is 20° C.-23° C., and under such temperature conditions, the growth rate of the crop can be accelerated, the health of the root system of the crop can be improved, and the yield and quality of the crop can be ensured. Illustratively, the environmental temperature for hydroponic intensive cultivation may be any point value, such as 20° C., 21° C., 22° C., or 23° C., or an interval value between any two point values, but is not limited thereto.

[0095] In some possible embodiments, the environmental relative humidity for hydroponic intensive growth is 50%-60%, which can suppress the growth of pathogens, prevent infection of the crop root system, and prevent dehydration and wilting of the crop. This is more beneficial for crop photosynthesis and promotes crop growth and development. For example, the environmental relative humidity for hydroponic intensive growth may be any typical but non-limiting value, such as 50%, 52%, 54%, 55%, 56%, 58%, or 60%, or an interval value between any two values.

[0096] In some possible embodiments, the effective light dose during the hydroponic intensive growth stage is 200 μmol m -2 s -1 −250 μmol m -2 s -1 Light irradiation affects the photosynthesis of hydroponic crops, which in turn affects their demand for and absorption of trace elements. Under such effective light conditions, the absorption rate of trace elements in the hydroponic crop growth solution can be more effectively increased. For example, the effective light intensity during the hydroponic intensive growth stage is 200 μmol m -2 s -1 , 210 μmol m -2 s -1 , 220 μmol m -2 s -1 , 230 μmol m -2 s -1 , 240 μmol m -2 s -1 , 250 μmol m -2 s -1 The value may be any point value or an interval value between any two point values, which are typical but not limited to the above.

[0097] In some possible embodiments, the photoperiod for the hydroponic intensive growth stage is 14-18 hours of light and 6-10 hours of darkness. Under these photoperiod conditions, sufficient photosynthesis is provided for crop growth, thereby accumulating sufficient energy to promote crop growth and trace element absorption. Exemplarily, the photoperiod for the hydroponic intensive growth stage may be any point value or interval value between any two point values, such as, but not limited to, 14 hours of light and 6 hours of darkness, 15 hours of light and 8 hours of darkness, 16 hours of light and 10 hours of darkness, 16 hours of light and 8 hours of darkness, 15 hours of light and 7 hours of darkness, or 14 hours of light and 9 hours of darkness.

[0098] In some possible embodiments, the ratio of white light:blue light:red light in the hydroponic intensive growth stage is (1-1.5):1:(4-5). Such a hydroponic growth process is more favorable for crop growth and trace element absorption. For example, the ratio of white light:blue light:red light in the hydroponic intensive growth stage may be any typical but non-limiting value or interval value between any two point values, such as 1.2:1:4.5, 1:1:4, 1:1:5, 1.2:1:5, 1.4:1:4, 1.4:1:4.5, 1.4:1:5, 1.5:1:4, 1.5:1:4.5, 1.5:1:5, etc.

[0099] In some possible embodiments, hydroponic enrichment includes adding trace elements to a basal growth solution to prepare an enriched growth solution with a content of each trace element of 2.5 μM-5 mM, growing hydroponic crops in the enriched growth solution 7-10 days before harvesting, and periodically monitoring and replenishing the enriched growth solution for element type, element content, pH value, and conductivity. Under such conditions, crop growth and enhanced absorption of trace nutrient elements are favorable.

[0100] In a second aspect, an embodiment of the present application provides a hydroponic, nutritionally enhanced crop obtained by growing using the above-described method for growing a hydroponic, nutritionally enhanced crop, wherein the content of trace elements in the hydroponic, nutritionally enhanced crop is higher than the content of trace elements in a hydroponic, basal-grown crop.

[0101] The hydroponic nutrient-enhanced crops according to the examples of the present application are grown using the above-mentioned method for growing hydroponic nutrient-enhanced crops, and the trace element content of the hydroponic nutrient-enhanced crops is higher than that of the hydroponic basal crops. By eating these hydroponic nutrient-enhanced crops, the human body can be better replenished with the trace elements required.

[0102] In order to make the details and operations of the above implementations of the present application clearly understandable to those skilled in the art, and to prominently embody the inventive step of the hydroponic nutrient-enhanced crops and the cultivation methods thereof in the embodiments of the present application, the above technical solutions are exemplified below by several embodiments.

[0103] Example 1 The cultivation of hydroponic Chinese cabbage fortified with iodine element includes the following steps:

[0104] 1. Seedling cultivation: Approximately 200 seeds were selected, their surfaces sprayed with absolute alcohol, and they were left in a ventilated area for 24 hours. After treatment, the seeds were placed in a medium cup containing a seedling sac, and an appropriate amount of secondary distilled water was added. During seedling cultivation, the seeds were covered with a plastic cover to maintain humidity, and an LED lamp was used as the light source, providing an average photosynthetically active light (PPFD) of 60 μmol m -2 s -1 The temperature was set to 12h / 12h (light / dark) and the photoperiod was set to 12h / 12h. When the seedlings grew to two leaflets, those in good condition were selected and transferred to a hydroponic system for further cultivation.

[0105] 2. Preparation of the basal growth solution: Use a compound nutrient powder with a nitrogen:phosphorus:potassium ratio of 10:5:14. Add the compound nutrient powder to double distilled water, and adjust the conductivity and pH to 1 dSm. -1 -2dSm -1 and adjusted to a pH range of 5.5-6.5.

[0106] 3. Hydroponic basic cultivation: During the cultivation period, Chinese cabbage seedlings were grown in a hydroponic system. The indoor temperature and relative humidity were maintained at 22±1°C and 50%-60%, respectively. The average photosynthetically active light output of the LED lamp was PPFD = 230 μmo m -2 s -1 The light ratio was adjusted to 1.2:1:4.5, and the photoperiod was adjusted to 16 h / 8 h (light / dark). The pH and conductivity of the basal growth solution were monitored every two days, and the pH was 5.5-6.5 and 1 dSm. -1 -2dSm -1 After each week, new basal growth solution was added.

[0107] 4. Preparation and cultivation of iodine-enriched growth solution: Potassium iodate was added to the basal growth solution so that the final concentration range of potassium iodate in the growth solution was stable at 2.5 μM. Each concentration had 15 independent replicate samples (i.e., 15 Chinese cabbage plants). After 35 days of cultivation, the basal growth solution was replaced with iodine-enriched growth solution, and daily monitoring and supplementation were performed. Growth continued under hydroponic basal growth conditions until the 45th day, at which point cultivation was terminated and iodine-enriched hydroponic Chinese cabbage was obtained.

[0108] Example 2 The difference between the iodine-enriched hydroponic cabbage and the cultivation method of Example 1 is that in step 4, the final concentration of potassium iodate in the iodine-enriched cultivation solution is stabilized at 5 μM.

[0109] Example 3 The difference between the iodine-enriched hydroponic cabbage and the cultivation method in Example 1 is that in Step 4, the final concentration range of potassium iodate content in the iodine-enriched cultivation solution is stabilized at 10 μM.

[0110] Example 4 The difference between the iodine-enriched hydroponic cabbage and the cultivation method in Example 1 is that in Step 4, the final concentration range of potassium iodate in the iodine-enriched cultivation solution is stabilized at 20 μM.

[0111] Example 5 The difference between the iodine-enriched hydroponic cabbage and the cultivation method in Example 1 is that in Step 4, the final concentration range of potassium iodate in the iodine-enriched cultivation solution is stabilized at 80 μM.

[0112] Example 6 Hydroponic butter lettuce fortified with calcium elements, the cultivation of which includes the following steps:

[0113] 1. Seedling cultivation: Approximately 200 seeds were selected, their surfaces sprayed with absolute alcohol, and they were left in a ventilated area for 24 hours. After treatment, the seeds were placed in a medium cup containing a seedling sac, and an appropriate amount of secondary distilled water was added. During seedling cultivation, the seeds were covered with a plastic cover to maintain humidity, and an LED lamp was used as the light source, providing an average photosynthetically active light (PPFD) of 60 μmol m -2 s -1 The temperature was set to 12h / 12h (light / dark) and the photoperiod was set to 12h / 12h. Once the seedlings had grown to two leaflets, those in good condition were selected and moved to a hydroponic facility for further cultivation.

[0114] 2. Preparation of the basal growth solution: Use a compound nutrient powder with a nitrogen:phosphorus:potassium ratio of 10:5:14. Add the compound nutrient powder to double distilled water, and adjust the conductivity and pH to 1 dSm. -1 -2dSm -1 and adjusted to a pH range of 5.5-6.5.

[0115] 3. Hydroponic basic cultivation: While butter lettuce seedlings were cultivated in the hydroponic system, the indoor ambient temperature and relative humidity were maintained at 22±1°C and 50%-60%, respectively. The average photosynthetically active light output of the LED lamp was PPFD=230 μmol m -2 s -1 The light ratio was adjusted to 1.2:1:4.5, and the photoperiod was adjusted to 16 h / 8 h (light / dark). The pH and conductivity of the basal growth solution were monitored every two days, and the pH was 5.5-6.5 and 1 dSm. -1 -2dSm -1 After each week, new basal growth solution was added.

[0116] 4. Preparation and cultivation of calcium-enriched growth solution: Calcium chloride was added to the basal growth solution, and the final calcium chloride concentration range of the growth solution was stabilized at 0.9 mM. At each concentration, 15 independent replicate samples (i.e., 15 butter lettuce plants) were used. After cultivating the butter lettuce for 23 days, the basal growth solution was replaced with a calcium-enriched growth solution, and daily monitoring and supplemental consumption were performed. Growth continued under the hydroponic basal growth conditions until the 30th day, at which point cultivation was terminated, yielding calcium-enriched hydroponic butter lettuce.

[0117] Example 7 The difference between the calcium-enriched hydroponic butter lettuce and the cultivation method in Example 1 is that in Step 4, the calcium chloride content in the calcium-enriched cultivation solution is stabilized at a final concentration range of 1.8 mM.

[0118] Example 8 The difference between the calcium-enriched hydroponic butter lettuce and the cultivation method in Example 1 is that in Step 4, the calcium chloride content in the calcium-enriched cultivation solution is stabilized at a final concentration range of 2.7 mM.

[0119] Example 9 The difference between the calcium-enriched hydroponic butter lettuce and the cultivation method in Example 1 is that in Step 4, the calcium chloride content in the calcium-enriched cultivation solution is stabilized at a final concentration range of 3.6 mM.

[0120] Example 10 The difference between the calcium-enriched hydroponic butter lettuce and the cultivation method in Example 1 is that in Step 4, the calcium chloride content in the calcium-enriched cultivation solution is stabilized at a final concentration range of 4.5 mM.

[0121] Example 11 The hydroponic cabbage fortified with iron element is grown by the following steps:

[0122] 1. Seedling cultivation: Approximately 200 Chinese cabbage seeds were selected, their surfaces sprayed with absolute alcohol, and left in a ventilated area for 24 hours. After treatment, the seeds were placed in a medium cup containing a seedling sac, and an appropriate amount of secondary distilled water was added. During seedling cultivation, the seeds were covered with a plastic cover to maintain humidity, and an LED lamp was used as the light source, providing an average photosynthetically active light (PPFD) of 60 μmol m -2 s -1 The temperature was set to 12h / 12h (light / dark) and the photoperiod was set to 12h / 12h. Once the seedlings had grown to two leaflets, those in good condition were selected and moved to a hydroponic facility for further cultivation.

[0123] 2. Preparation of the basal growth solution: Use a compound nutrient powder with a nitrogen:phosphorus:potassium ratio of 10:5:14. Add the compound nutrient powder to double distilled water, and adjust the conductivity and pH to 1 dSm. -1 -2dSm -1 and adjusted to a pH range of 5.5-6.5.

[0124] 3. Hydroponic basic cultivation: During the cultivation period, Chinese cabbage seedlings were grown in a hydroponic facility. The indoor ambient temperature and relative humidity were maintained at 22±1°C and 50%-60%, respectively. The average photosynthetic light output of the LED lamp was PPFD=230 μmol m -2 s -1 The light ratio was adjusted to 1.2:1:4.5, and the photoperiod was adjusted to 16 h / 8 h (light / dark). The pH and conductivity of the basal growth solution were monitored every two days, and the pH was 5.5-6.5 and 1 dSm. -1 -2dSm -1 After each week, new basal growth solution was added.

[0125] 4. Preparation and cultivation of iron-enriched growth solution: Diethylenetriaminepentaacetic acid (Fe-DTPA) was added to the basal growth solution, and the final concentration range of Fe-DTPA content in the growth solution was stabilized at 0.25 mM. Each concentration had 15 independent replicate samples (i.e., 15 Chinese cabbage plants). After the cabbages were grown for 23 days, the basal growth solution was replaced with iron-enriched growth solution, and daily monitoring and supplemental consumption were performed. Growth continued under the hydroponic basal growth conditions until the 30th day, at which point cultivation was terminated, and iron-enriched hydroponic Chinese cabbage was obtained.

[0126] Example 12 The difference between the iron-enriched hydroponic cabbage and Example 11 in the cultivation method is that in step 4, the final concentration of Fe-DTPA in the calcium-enriched cultivation solution is stabilized at 0.5 mM.

[0127] Example 13 The difference between the iron-enriched hydroponic cabbage and that of Example 11 is that in step 4, the final concentration of Fe-DTPA in the calcium-enriched culture solution is stabilized at 0.7 mM.

[0128] Example 14 The difference between the iron-enriched hydroponic cabbage and the cultivation method of Example 11 is that in step 4, the final concentration of Fe-DTPA in the calcium-enriched cultivation solution is stabilized at 1 mM.

[0129] Example 15 The difference between the iron-enriched hydroponic cabbage and Example 11 in the cultivation method is that in step 4, the final concentration of Fe-DTPA in the calcium-enriched cultivation solution is stabilized at 2 mM.

[0130] Comparative Example 1 This is iodine-enriched hydroponic cabbage, and the difference between this and Example 1 in the cultivation method is that iodine-enriched cultivation was not performed, and the cabbage was grown using basic hydroponic cultivation until the 45th day, at which point cultivation was terminated and hydroponic cabbage was obtained.

[0131] Comparative Example 2 This is calcium-enriched hydroponic butter lettuce, and its cultivation method differs from that of Example 6 in that calcium-enriched cultivation was not performed, and the lettuce was grown using basic hydroponic cultivation until the 30th day, at which point cultivation was terminated and hydroponic butter lettuce was obtained.

[0132] Comparative Example 3 This is an iron-enriched hydroponic cabbage, and its cultivation method differs from that of Example 11 in that it was not subjected to iron-enriched cultivation, but was grown using basic hydroponic cultivation until the 30th day, at which point cultivation was terminated and hydroponic cabbage was obtained.

[0133] In order to verify the inventive step of the examples of the present application, the hydroponic vegetables grown in the above examples are subjected to the following performance tests.

[0134] 1. The morphology, yield and trace element content of the hydroponic Chinese cabbages grown in Examples 1-5 and Comparative Example 1 were measured.

[0135] (1) The hydroponic Chinese cabbage grown in Example 1 was completed on the 45th day, and photographs were taken and recorded. The measurement results are shown in FIG. 2.

[0136] (2) The edible portions of the hydroponic Chinese cabbages grown in Examples 1 to 5 and Comparative Example 1 were harvested together and weighed, and the wet weight of each group was recorded. The measurement results are shown in FIG. 2.

[0137] (3) The edible leaf portions of the hydroponic cabbages grown in Examples 1-5 and Comparative Example 1 were further dried overnight in a 75°C oven. After the oven temperature stabilized, the temperature was raised to 105°C and dried for three days to remove moisture from all leaf portions, which were then used to measure iodine content. 1 mL of tetramethylammonium hydroxide and 5 mL of ultrapure water were added to each hydroponic cabbage sample (0.5 g dry weight) grown in Examples 1-5 and Comparative Example 1, and alkaline hydrolysis was performed at 90°C for three hours. The resulting hydrolyzed solution was added to a 25 mL volumetric flask with ultrapure water and centrifuged (4000 rpm; 10 min). The supernatant was passed through a 0.45 μm filter membrane, and a reference gene standard solution (final concentration 5 ppb) was added. The iodine content was then measured by ICP-MS. The measurement results are shown in FIG. 4 (*P<0.05, **P<0.01, ***P<0.001, ****P<0.0001 vs. Comparative Example 1).

[0138] As can be seen from the measurement results in Figures 2 and 3, the iodine fortification technique does not adversely affect the quality (i.e., yield and morphology) of hydroponic Chinese cabbage.

[0139] As can be seen from the measurement results in Figure 4, the iodine content of the hydroponic cabbage grown using the iodine element fortification technology of Examples 1-5 of the present application was significantly improved compared to the iodine content (0.12 μg iodine / g wet weight) of the hydroponic cabbage grown in Comparative Example 1. The iodine content of the hydroponic cabbage grown in Example 5 was 3.96 μg iodine / g wet weight, which is 33 times the iodine content (0.12 μg iodine / g wet weight) of the hydroponic cabbage grown in Comparative Example 1. According to the World Health Organization's recommendations, the recommended daily iodine intake for adults is approximately 120 μg. Therefore, consuming approximately 30.3 g of the iodine-fortified hydroponic cabbage grown in Example 5 of the present application daily would meet the recommended daily iodine intake. To consume the conventional hydroponic cabbage of Comparative Example 1, 1 kg would be required.

[0140] 2. The morphology, yield and trace element content of the hydroponic butter lettuce grown in Examples 6-10 and Comparative Example 2 were measured.

[0141] (1) The hydroponic butter lettuce grown in Example 6 was completed, photographed, and recorded. The measurement results are shown in FIG.

[0142] (2) The edible portions of the hydroponic butter lettuce grown in Examples 6 to 10 and Comparative Example 2 were harvested collectively and weighed, and the wet weight of each group was recorded. The measurement results are shown in Figure 6.

[0143] (3) The edible leaves of the hydroponic butter lettuce grown in Examples 6-10 and Comparative Example 2 were further dried overnight in a 75°C oven. After the oven temperature stabilized, the temperature was raised to 105°C and dried for three days to remove moisture from all leaves, which were then used to measure iodine content. Samples (0.5 g dry weight) of hydroponic butter lettuce grown in Examples 6-10 and Comparative Example 2 were placed in a microwave digestion tank, and 10 mL of concentrated nitric acid was added and acid hydrolysis was performed using a microwave digestion device. After cooling, the digested solution was placed in a 25 mL volumetric flask and the volume was adjusted to volume with ultrapure water. The samples were then passed through a 0.45 μm filter membrane and the calcium content was further measured using ICP-OES. The measurement results are shown in Figure 7 (*P<0.05, **P<0.01, ***P<0.001, ****P<0.0001 vs. Comparative Example 2).

[0144] As can be seen from the measurement results in Figures 5 and 6, the calcium element enrichment technique does not adversely affect the quality (i.e., yield and morphology) of hydroponic butter lettuce.

[0145] As can be seen from the measurement results in Figure 7, the calcium content of the hydroponic butter lettuce grown in Comparative Example 2 (570 μg calcium / g wet weight) was significantly improved in the hydroponic butter lettuce grown in Examples 6-10 of the present application using calcium enrichment. The calcium content of the hydroponic butter lettuce grown in Example 10 was 829 μg calcium / g wet weight, a 45.4% increase over the calcium content of the hydroponic butter lettuce grown in Comparative Example 1. The World Health Organization (WHO) recommends that an adult should consume approximately 1000 mg-1300 mg of calcium daily. Therefore, consuming approximately 1.21 kg-1.57 kg of the calcium-enriched hydroponic butter lettuce grown in Example 10 daily would meet the recommended daily calcium intake, while consuming 1.75 kg-2.28 kg of the hydroponic butter lettuce grown in Comparative Example 2 would be sufficient.

[0146] 3. The yield and trace element contents of the hydroponic Chinese cabbages grown in Examples 11 to 14 and Comparative Example 3 were measured.

[0147] (1) The edible portions of the hydroponic Chinese cabbages grown in Examples 11 to 14 and Comparative Example 3 were harvested collectively and weighed, and the wet weight of each group was recorded. The measurement results are shown in FIG.

[0148] (3) The edible leaf portions of the hydroponic cabbages grown in Examples 11-14 and Comparative Example 3 were further dried overnight in a 75°C oven. After the oven temperature stabilized, the temperature was raised to 105°C and dried for three days to remove moisture from all leaf portions, which were then used to measure iron content. Hydroponic cabbage samples (0.5 g dry weight) grown in Examples 11-14 and Comparative Example 3 were placed in a microwave digestion tank, and 10 mL of concentrated nitric acid was added and acid hydrolysis was performed using a microwave digestion device. After cooling, the digested solution was placed in a 25 mL volumetric flask and the volume was adjusted to volume with ultrapure water. The sample was passed through a 0.45 μm filter membrane and the iron content was measured using ICP-OES. The measurement results are shown in Figure 9 (*P<0.05, **P<0.01, ***P<0.001, ****P<0.0001 vs. Comparative Example 3).

[0149] As can be seen from the measurement results in Figure 8, iron element enrichment technology does not have a negative effect on the yield or quality of hydroponic Chinese cabbage.

[0150] As can be seen from the measurement results in Figure 9, the iron content of the hydroponic cabbage grown in Example 11-14 using the iron-enriched technology was significantly improved compared to the iron content (4.1 μg iron / g wet weight) of the hydroponic cabbage grown in Comparative Example 3. The iron content of the hydroponic cabbage grown in Example 14 was 8.6 μg iron / g wet weight, which is twice the iron content (4.1 μg iron / g wet weight) of the hydroponic cabbage grown in Comparative Example 3. According to the World Health Organization's recommendations, the recommended daily iron intake for adults is approximately 12 mg-20 mg. Therefore, daily consumption of the iron-enriched hydroponic cabbage grown in Example 14 can significantly reduce the difficulty of ingesting iron from vegetables.

[0151] The above are merely preferred embodiments of the present application, and are not intended to limit the present application. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present application should be included within the scope of protection of the present application.

Claims

1. A method for growing hydroponic nutrient-enhanced crops, comprising: A step of obtaining seeds to be cultivated, cultivating the seeds, and obtaining the seeds; A step of preparing a basal growth solution containing nitrogen, phosphorus and potassium elements, and performing hydroponic basal growth on the seedling to obtain a hydroponic crop; A method for growing hydroponic nutritionally enhanced crops, comprising the steps of preparing an enriched growth solution having a higher trace element content than the trace element content in the basal growth solution, and performing hydroponic enrichment growth on the hydroponic crops to obtain the hydroponic nutritionally enhanced crops.

2. The enriched growth solution contains nitrogen, phosphorus, and potassium, and also contains at least one trace element selected from calcium, iodine, iron, selenium, zinc, manganese, and copper, And / or, in the enriched growth solution, the content of each of the trace elements is 2.5 μM or more; And / or the conductivity of the strengthened growth solution is 1 d / Sm-2 d / Sm; And / or, the pH value of the enriched growth solution is 5.5-6.5, The method for growing hydroponic enriched crops according to claim 1.

3. In the enriched growth solution, the mass concentration of the nitrogen element is 8 g / L-12 g / L, the mass concentration of the phosphorus element is 4 g / L-6 g / L, the mass concentration of the potassium element is 12 g / L-16 g / L, and the contents of various trace elements are 2.5 μM-5 mM; And / or, in the strengthened growth solution, the content of the iodine element is 2.5 μM-80 μM, And / or, in the enriched growth solution, the content of the calcium element is 0.9 mM-5 mM, And / or, in the strengthened growth solution, the content of the iron element is 0.25 mM-2 mM, and / or the hydroponic intensive growing is carried out 7-10 days before harvesting the hydroponic crop; And / or, the environmental temperature of the hydroponic intensive growth is 20 ° C to 23 ° C, And / or the environmental relative humidity of the hydroponic intensive growth is 50%-60%; And / or, the effective light amount in the hydroponic intensive growth stage is 200 μmol m －２ s －１ -250 μmol m －２ s －１ and And / or, the photoperiod of the hydroponic intensive growth stage is 14 hours to 18 hours of light and 6 hours to 10 hours of darkness; And / or, the ratio of white light: blue light: red light in the hydroponic enrichment cultivation stage is (1-1.5): 1: (4-5).

4. In the basal growth solution, the molar ratio of the nitrogen element to the phosphorus element to the potassium element is (8-12):(4-6):(12-16), and / or, in the basal growth solution, the mass concentration of the nitrogen element is 8 g / L to 12 g / L, the mass concentration of the phosphorus element is 4 g / L to 6 g / L, and the mass concentration of the potassium element is 12 g / L to 16 g / L; and / or the basal growth solution further contains at least one element selected from the group consisting of calcium, magnesium, sulfur, iron, and manganese; and / or the conductivity of the basal growth solution is 1 d / Sm-2 d / Sm; And / or, the pH value of the basal growth solution is 5.5-6.5, The method for growing hydroponic nutrient-enriched crops according to claim 1.

5. The environmental temperature for the hydroponic basic cultivation is 20°C-23°C, And / or the environmental relative humidity of the hydroponic base cultivation is 50%-60%, And / or, the effective light amount in the hydroponic basic cultivation stage is 200 μmol m －２ s －１ -250 μmol m －２ s －１ and And / or, the photoperiod during the hydroponic basic growth stage is 14 hours to 18 hours of light / 6 hours to 10 hours of darkness; And / or, the ratio of white light: blue light: red light in the hydroponic basic cultivation stage is (1-1.5): 1: (4-5).

6. The effective light intensity at the seedling cultivation stage is 40 μmol m －２ s －１ -80 μmol m －２ s －１ and And / or, the photoperiod during the seedling growth stage is 10 hours - 14 hours of light / 10 hours - 14 hours of darkness, and / or the ratio of blue light to red light during the seedling development stage is (1.5-2):1; And / or the method for growing hydroponic nutrient-enhanced crops according to any one of claims 1 to 3 and 5, characterized in that the seeds to be grown are selected from vegetable seeds.

7. The seedling cultivation was carried out by sterilizing the target seeds with alcohol, placing them in a cultivation cup containing a seedling sac, adding secondary distilled water to cover a protective film, and then irradiating the seeds with an effective light intensity of 60 μmol m －２ s －１ and growing the seeds until they have two leaflets under conditions where the photoperiod is 12 hours light / 12 hours dark with a blue light:red light ratio of 1.7:1, and then selecting the seeds for hydroponic cultivation.

8. The hydroponic basal culture was performed by cultivating the seedlings in a hydroponic facility using the basal culture solution having a molar ratio of nitrogen, phosphorus, and potassium of 10:5:14, at an ambient temperature of 20°C to 23°C, a relative humidity of 50% to 60%, and an effective light intensity of 230 μmol m －２ s －１ and growing the crops under conditions of a photoperiod of 16 hours of light / 8 hours of darkness with a white light:blue light:red light ratio of 1.2:1:4.5, periodically monitoring and maintaining the electrical conductivity of the basal growth solution at 1 d / Sm-2 d / Sm and the pH value at 5.5-6.5, and replacing the basal growth solution once every week.

9. The hydroponic enriched cultivation method of claim 8 includes the steps of adding the trace elements to the basal cultivation solution to prepare an enriched cultivation solution with a content of each of the trace elements of 2.5 μM to 5 mM, cultivating the hydroponic crops using the enriched cultivation solution 7 to 10 days before harvesting the hydroponic crops, and periodically detecting and replenishing the type of elements, the content of elements, pH value, and conductivity of the enriched cultivation solution.

10. A hydroponic nutritionally enhanced crop, wherein the content of trace elements in the hydroponic nutritionally enhanced crop is higher than the content of trace elements in a hydroponic basal grown crop.

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