Composition for enhancing plant disease resistance

Abstract

The present application relates to a composition for enhancing plant disease resistance containing an amino acid, a method for enhancing plant disease resistance using the same, a method for preventing or treating plant disease, use for enhancing plant resistance, use for preventing or treating plant disease, a composition for plant control containing an amino acid, a method for plant control using the same, and use for plant control.

Description

[Technical field]

[0001] The present application relates to a composition for enhancing plant disease resistance containing an amino acid, a method for enhancing plant disease resistance using the same, a method for preventing or treating plant disease, use for enhancing plant resistance, use for preventing or treating plant disease, a composition for plant control containing an amino acid, a method for plant control using the same, and use for plant control. [Background technology]

[0002] Plants have developed signal transduction systems that allow them to effectively respond to changes in the external environment. For example, when a plant is exposed to a pathogen, a resistance response induces expression of resistance-related genes, activating the signal transduction system. In addition, plant hormones such as jasmonic acid, ethylene, and salicylic acid are known to play a major role in inducing induced resistance from the site of infection throughout the plant. Salicylic acid and jasmonic acid move throughout the plant in their own or derived forms, respectively, and induce a systemic resistance response. This induction of a systemic response induces a resistance response not only in leaves invaded by pathogens, but also throughout the plant body, even in parts that have not been invaded.

[0003] In relation to this, derivatives that induce various resistance reactions, not only against pathogens, have been reported. Among them, BTH (benzo(1,2,3)-thiadiazile-7-carbothioic acid S-methyl ester, benzothiadiazole) is known to be the most effective. However, treatment with BTH has serious problems, such as suppressing plant growth and significantly reducing yields. Subsequent research has shown that this problem is due to the fact that BTH expresses many resistance genes in plants even in the absence of pathogens, resulting in excessive consumption of energy by the plant and a lack of energy for growth. Therefore, there is an urgent need to develop a substance that has an effect similar to BTH in inducing a systemic resistance reaction, but does not cause plant growth inhibition.

[0004] In addition, although research has been conducted on agricultural and horticultural fungicides and insecticides that contain certain compounds (Patent Document 1), the development of substances that induce a systemic resistance response without suppressing plant growth remains insufficient. [Prior art documents] [Patent documents]

[0005] [Patent Document 1] U.S. Patent No. 10,123,537 [Non-patent literature]

[0006] [Non-Patent Document 1] Denoux et al., Molecular Plant, Volume 1, Number 3, pages 423-445, May 2008 Summary of the Invention [Problem to be solved by the invention]

[0007] The problem to be solved by the present application is to provide a composition for enhancing plant disease resistance containing an amino acid, a method for enhancing plant disease resistance using the same, a method for preventing or treating plant disease, use for enhancing plant resistance, use for preventing or treating plant disease, a plant control composition containing an amino acid, a method for plant control using the same, and use for plant control. [Means for solving the problem]

[0008] An object of the present application is to provide a composition for enhancing plant disease resistance, which contains an amino acid.

[0009] Another object of the present application is to provide a method for enhancing plant disease resistance using the composition for enhancing plant disease resistance.

[0010] A further object of the present application is to provide a method for preventing or treating a plant disease using the composition for enhancing plant disease resistance.

[0011] A further object of the present application is to provide a plant control composition comprising an amino acid.

[0012] A further object of the present application is to provide a plant control method using the plant control composition.

[0013] A further object of the present application is to provide use of an amino acid or the composition for enhancing plant disease resistance for enhancing plant disease resistance.

[0014] A further object of the present application is to provide a use of the amino acid or said plant control composition for plant control.

[0015] A further object of the present application is to provide use of an amino acid, the composition for enhancing plant disease resistance, or the composition for plant control for preventing or treating a plant disease. Effect of the Invention

[0016] The use of amino acids has the effect of enhancing the disease resistance of plants. [Brief description of the drawings]

[0017] [Figure 1] FIG. 1 shows the results of verifying induced resistance of 10 types of amino acids in a model plant (Arabidopsis thaliana). [Diagram 2] FIG. 1 shows the results of determining the valine concentration at which induced resistance is most excellent in a model plant. [Diagram 3] FIG. 1 shows that pathogens are reduced by treating model plants with valine, proline and isoleucine. [Figure 4] FIG. 1 shows the results of observing the level of disease resistance gene expression in model plants upon treatment with valine, proline, and isoleucine. [Diagram 5] FIG. 1 is a graph confirming the control effect of treatment with valine and isoleucine on soybean, cucumber, lettuce, rice, tomato, wheat, barley and chili pepper. [Figure 6] FIG. 1 is a graph confirming the control effect of treatment with valine, proline, and isoleucine on soybean, cucumber, and lettuce. [Figure 7] FIG. 1 shows the results of an induced resistance test using a hyperspectral camera. [Figure 8] FIG. 1 shows the results of an assay of the growth-promoting ability of valine. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0018] The present application will be described in detail below. Note that the description and embodiment of one aspect disclosed in this application also apply to the description and embodiment of other aspects with respect to common matters. In addition, all combinations of various elements disclosed in this application are included in this application. Furthermore, the present application is not limited to the following specific description.

[0019] One aspect of the present application provides a composition for enhancing plant disease resistance, comprising an amino acid.

[0020] The amino acid is at least one selected from the group consisting of branched-chain amino acids, proline, glutamic acid, aspartic acid, and histidine, and the plant disease is at least one selected from the group consisting of leaf spot, bacterial soft rot, Phytophthora stem and root rot, bacterial leaf blight, and rice blast.

[0021] In the present application, the term "branched chain amino acid" refers to an amino acid having a branched alkyl group in the side chain, including valine, leucine, and isoleucine. Specifically, in the present application, the branched chain amino acid is an L-branched chain amino acid, and the L-branched chain amino acid is at least one selected from L-valine, L-leucine, and L-isoleucine, but is not limited thereto.

[0022] The proline is L-proline, the glutamic acid is L-glutamic acid, the aspartic acid is L-glutamic acid, and the histidine is L-histidine, but is not limited thereto.

[0023] As an example, the amino acid of the present application is used to treat a plant at a concentration of 0.0001-10%, 0.0001-5%, 0.0001-1%, 0.005-10%, 0.005-1%, 0.005-0.5%, 0.001-10%, 0.001-1%, 0.001-0.5%, 0.01-10%, 0.01-1%, 0.01-0.5%, 0.05-10%, 0.05-1%, 0.05-0.5%, 0.1-10%, 0.1-1%, or 0.1-0.5% (w / w) based on the entire composition for enhancing plant disease resistance, the entire dilution thereof, or the entire concentrate, but is not limited thereto.

[0024] The present application has a technical significance in that it has elucidated that amino acids, specifically branched-chain amino acids, proline, glutamic acid, aspartic acid and histidine, have uses in enhancing plant disease resistance or in plant control.

[0025] In this application, the term "plant disease" refers to a disease that occurs in plants, and means that abnormalities occur in the original morphology or physiological functions. The cause of the disease is a disease caused by a pathogen, a nutrient deficiency in the soil, weather conditions, toxic gases, etc. The pathogen is a fungal pathogen or a bacterial pathogen, but is not limited thereto.

[0026] Specifically, the plant disease of the present application is at least one selected from the group consisting of leaf spot, bacterial soft rot, Phytophthora stem and root rot, bacterial leaf blight, and rice blast.

[0027] In the present application, the term "leaf spot disease" refers to a disease in which large and small spots (spots or specks) appear on the trunk, fruit, and leaves of a plant body. The spots are black, light brown, or brown. The spot disease mainly affects fruit vegetables, but can affect any type of plant. The spot disease is caused by various types of fungi or bacteria, and is induced by a parasitic pathogen of a living plant, specifically, a microorganism of the genus Pseudomonas, more specifically, Pseudomonas syringae pv. tomato, and even more specifically, Pseudomonas syringae pv. tomato DC3000, but is not limited thereto.

[0028] In this application, "bacterial soft rot" refers to a disease in which the tissues of a plant are eroded, causing a foul odor, rotting, and sores. It is particularly prevalent in vegetables with high moisture content, such as lettuce and Chinese cabbage, but it can also occur in tomatoes, sweet potatoes, potatoes, etc., so it can occur in any type of plant. The soft rot is induced by necrotic parasitic bacteria, specifically, Pectobacterium carotovorum subsp. carotovorum, but is not limited thereto.

[0029] In this application, "Phytophthora stem and root rot" is a type of fungal disease that affects potatoes, tomatoes, etc., and the external symptoms vary depending on the degree of infection. The plant body in the vigorous growth period shrinks, the lower leaves take on a reddish-brown color, and the young leaves fade and take on various colors. When the infection is not severe, the external symptoms are often not clear, the fruits of the diseased plants are small, and the stolon formation is poor. The Phytophthora stem and root rot mainly affects fruit vegetables, but can affect any type of plant. The Phytophthora stem and root rot is induced by a pathogenic fungus of the genus Phytophthora, which belongs to the Pythiaceae family of flagellate fungi, specifically, Phytophthora sojae, but is not limited thereto.

[0030] In this application, "bacterial leaf blight" refers to a disease in which spots appear and turn brown. The spots are formed on leaves, stems, pods, petioles, cotyledons, etc. Any type of plant may develop the disease, including soybeans, mung beans, and fruit vegetables. The bacterial leaf blight is induced by a microorganism of the genus Pseudomonas, specifically, Pseudomonas syringae pv. lachrymans, but is not limited thereto.

[0031] In this application, "rice blast" is the most important disease of cereals, especially rice, and occurs in all parts of cereals. Lesions form on aboveground parts such as leaves, panicles, stalks, nodes, and rice grains, with blast occurring most frequently on leaves, panicles, and stalks. Infection occurs by conidia, and the general symptoms are that irregular red, gray, black, and / or brown spots spread on the leaves, and the entire leaf turns brown and withers. The head of the panicle and stalks wither and turn light brown, and gray mold develops on the surface when there is a lot of moisture. Dark brown lesions form on the nodes, which become easily broken, and in humid environments the surface is covered with gray mold. The leaf spot disease is induced by, but is not limited to, microorganisms of the genus Magnaporthe, specifically Magnaporthe oryzae.

[0032] In one embodiment, the plant disease is caused by at least one fungus selected from the group consisting of, but not limited to, Pseudomonas syringae pv. tomato, Pectobacterium carotovorum subsp. carotovorum, Phytophthora sojae, Pseudomonas syringae pv. lachrymans, and Magnaporthe oryzae.

[0033] In one embodiment, the plant disease may be leaf spot caused by Pseudomonas syringae pv. tomato, soft rot caused by Pectobacterium carotovorum subsp. carotovorum, soybean stem rot caused by Phytophthora sojae, cucumber bacterial spot caused by Pseudomonas syringae pv. lachrymans, lettuce soft rot caused by Pectobacterium carotovorum subsp. carotovorum, and Magnaporthe oryzae. oryzae), but is not limited thereto.

[0034] The plant disease may be caused by any type of plant, and in one embodiment, the plant may be selected from the group consisting of beans, fruit vegetables, leafy vegetables, and cereals.

[0035] In the present application, "legumes" refers to legumes (Fabaceae) that are used as food or feed. Examples include soybean, wild bean, kidney bean, black bean, pea, adzuki bean, peanut, cowpea, etc. Specifically, the legume is soybean, but is not limited thereto.

[0036] In the present application, "fruit vegetables" refers to vegetables intended for use as fruits when classified according to the part of the vegetable that is used. Examples include chili peppers, cucumbers, tomatoes, watermelons, strawberries, and Japanese cantaloupe. Specifically, the fruit vegetables are chili peppers, cucumbers, and tomatoes, but are not limited thereto.

[0037] In the present application, "leafy vegetables" refers to vegetables intended for use as leaves when classified according to the part of the vegetable that is used. Examples include Chinese cabbage, lettuce, perilla, spinach, etc. Specifically, the leafy vegetables are Chinese cabbage and lettuce, but are not limited thereto.

[0038] In the present application, "cereals" refers to cultivated plants of the Gramineae family whose seeds are used for food. Examples include rice, wheat, barley, corn, etc. Specifically, the cereals are rice, wheat, or barley, but are not limited thereto.

[0039] In the present application, "resistance to plant diseases" means a property of preventing the invasion of pathogens, a property of preventing the onset of plant diseases, and / or a property of not becoming infected with a disease even if invaded by a pathogen.

[0040] In one specific example, the amino acid of the present application may be capable of preventing or treating pathogen infection in a plant, or of inducing systemic induced resistance in a plant, but is not limited thereto.

[0041] As a specific example, the amino acid of the present application enhances plant disease resistance and does not inhibit plant growth, but is not limited thereto. Specifically, derivatives that induce various resistance reactions, not just to pathogens, are known as substances that induce plant resistance, and some of these (e.g., benzo(1,2,3)-thiadiazole-7-carbothioic acid S-methyl ester, benzothiadiazole; BTH) cause serious side effects such as suppressing plant growth and significantly reducing yields. However, the amino acid of the present application does not suppress plant growth, but rather promotes plant growth.

[0042] The composition for enhancing resistance to plant diseases of the present application may be any formulation or product necessary for enhancing resistance to plant diseases, such as an agricultural chemical formulation, a fertilizer, or a control agent.

[0043] In this application, the term "pesticides" refers to chemicals used for the protection and growth of agricultural crops. Specifically, this term includes not only preparations used to control diseases, pests, weeds, pathogens, nematodes, mites, and the like that occur during the cultivation and storage of agricultural crops, but also growth regulators used to enhance or suppress the physiological functions of agricultural crops and adjuvants that enhance the efficacy of drugs.

[0044] The term "fertilizer" in this application means a nutrient material that enriches the soil and promotes the growth of plants. The fertilizer containing the composition of this application can be easily used by mixing it with the soil before sowing, and can be applied together with the application of pesticides and the like.

[0045] The composition for enhancing plant disease resistance of the present application may further contain substances that are commonly used for enhancing disease resistance, and may further contain, in addition to the amino acids, a pharma- ceutically acceptable solid carrier, liquid carrier, liquid diluent, liquefied gaseous diluent, solid diluent or other auxiliary agent as an excipient, such as a surfactant such as an emulsifier, dispersant, or foaming agent.

[0046] The composition for enhancing plant disease resistance of the present application can be formulated into an agricultural composition by mixing the active ingredient with the excipient, and the method for formulating the agricultural composition may be any method commonly used in the art.

[0047] In addition, the composition for enhancing plant disease resistance of the present application may be provided in the form of a wettable powder, granule, dust, emulsion, spray, smoke, capsule, or gel, or may be provided in a contact agent in a doughnut-shaped form for buoyancy of the composition, but is not limited thereto.

[0048] In one embodiment, the composition of the present application is a composition for enhancing resistance to or controlling a plant disease, comprising valine, and the plant disease is at least one selected from the group consisting of leaf spot, bacterial soft rot, Phytophthora root rot, bacterial leaf blight, and rice blast, but is not limited thereto.

[0049] In one embodiment, the composition of the present application is a composition for enhancing plant disease resistance or a composition for plant control, comprising isoleucine, and the plant disease is at least one selected from the group consisting of leaf spot, bacterial soft rot, Phytophthora root rot, bacterial leaf blight, and rice blast, but is not limited thereto.

[0050] As an example, but not limited to, the composition of the present application is a composition for enhancing resistance to leaf spot or bacterial soft rot or a composition for plant control, which contains leucine.

[0051] As an example, but not limited to, the composition of the present application is a composition for enhancing resistance to leaf spot or bacterial soft rot or a composition for plant control that contains proline.

[0052] As an example, the composition of the present application is a composition for enhancing resistance to spot disease or a composition for plant control that contains at least one selected from the group consisting of glutamic acid, aspartic acid, and histidine, but is not limited thereto.

[0053] Another aspect of the present application provides a method for enhancing resistance to plant diseases, comprising treating a plant body with a composition for enhancing resistance to plant diseases, the composition comprising an amino acid.

[0054] As a specific example, the method for enhancing plant disease resistance also promotes plant growth, but is not limited thereto.

[0055] The amino acids, plant diseases, plant disease resistance, and compositions for enhancing plant disease resistance are as described above.

[0056] The plant body may be any part of a plant, such as a root, a stem, a leaf, or a combination thereof, and may be any type of plant. Specifically, the plant body may be, but is not limited to, a plant individual that is at risk of being infected with a plant disease or is infected with a pathogen.

[0057] The treatment with the composition for enhancing plant disease resistance is carried out by spraying, injecting, pouring, mixing, or mixing the composition into soil, etc., of the plant body, but is not limited thereto, as long as the inside or outside of the plant body comes into direct or indirect contact with the composition. The treatment may be carried out simultaneously or at a different time when a pathogen invades the plant, or before or after the pathogen invades the plant, but is not limited thereto.

[0058] The composition for enhancing plant disease resistance is applied once or twice or more, but is not limited thereto. When applied twice or more, the application is performed at intervals of 3 to 15 days, 3 to 10 days, 3 to 7 days, 4 to 7 days, 5 to 7 days, or 6 days, but is not limited thereto.

[0059] In one embodiment, the composition for enhancing plant disease resistance is applied for a first treatment 7 days before the pathogen invasion and for a second treatment 1 day before the pathogen invasion, but is not limited thereto.

[0060] In one embodiment, the plant is at least one selected from the group consisting of beans, fruit vegetables, leafy vegetables, and grains, but is not limited thereto.

[0061] The beans, fruit vegetables, leafy vegetables, grains, etc. are as described above.

[0062] Yet another aspect of the present application provides a method for preventing or treating a plant disease, the method comprising the step of treating a plant body with a composition for enhancing resistance to a plant disease, the composition comprising an amino acid.

[0063] The amino acids, plant diseases, plant disease resistance, composition for enhancing plant disease resistance, treatment with the composition for enhancing plant disease resistance, plants, etc. are as described above.

[0064] In this application, "prevention" refers to any action that inhibits or delays the onset or progression of a plant disease.

[0065] In this application, "treatment" refers to any action that improves or beneficially alters the symptoms of a plant disease. In this application, treatment includes any action that prevents infection by a plant disease pathogen or the recurrence of a plant disease, alleviates symptoms, reduces the rate of progression, alleviates the disease state, temporarily or permanently alleviates the disease state, brings about recovery, or improves the prognosis.

[0066] Yet another aspect of the present application provides a plant control composition comprising an amino acid, the amino acid being at least one selected from the group consisting of branched-chain amino acids, proline, glutamic acid, aspartic acid, and histidine, and the plant disease being at least one selected from the group consisting of leaf spot, bacterial soft rot, Phytophthora root rot, bacterial leaf blight, and rice blast.

[0067] The amino acids, plant diseases, etc. are as described above.

[0068] In the present application, "control" means preventing damage to plants such as crops by pests, removing pathogens that have appeared, and preventing the spread of pathogens. The control is a series of actions for reducing damage caused by pests, and includes, but is not limited to, physical (mechanical) control methods, cultural control methods, chemical control methods, biological control methods, and environmentally friendly control methods. The control includes not only chemical control using fungicides or insecticides that are toxic to pests, but also methods of creating an environment that pathogens do not like by cultivating resistant plants, crop rotation, etc., methods of removing pathogens by heat treatment of plants or steam treatment of soil, and methods of attracting and killing pests using attractants or sex pheromones.

[0069] The plant control composition of the present application may be any formulation or commercial product necessary for plant control, such as agrochemical formulations, fertilizers, control agents, etc.

[0070] The plant control composition of the present application may further contain substances that are commonly used for plant control, and may further contain, in addition to the amino acids, a pharma- ceutically acceptable solid carrier, liquid carrier, liquid diluent, liquefied gaseous diluent, solid diluent or other auxiliary agent as an excipient, such as a surfactant, e.g., an emulsifier, a dispersant, or a foaming agent.

[0071] The plant control composition of the present application can be used by mixing the active ingredient with the excipient and formulating it into an agricultural composition, and the method for formulating it into an agricultural composition may be any method commonly used in the art.

[0072] The plant control composition of the present application may also be provided in the form of wettable powder, granules, dusts, emulsions, sprays, smokes, capsules and gels, and may also be provided in a contact formulation in a doughnut-shaped form to provide buoyancy for the composition.

[0073] Yet another aspect of the present application provides a method for controlling plants, the method comprising treating a plant with a plant control composition comprising an amino acid. Specifically, the method for controlling plants may also promote plant growth.

[0074] The amino acids, plants, plant bodies, plant diseases, control, plant control compositions, etc. are as described above.

[0075] The treatment with the plant control composition is carried out by spraying, injecting, pouring, mixing, or mixing the composition into soil, etc., as long as the inside or outside of the plant comes into contact with the composition, but is not limited to these. The treatment may be carried out simultaneously or at a different time when the pathogen invades the plant, or before or after the pathogen invades the plant, but is not limited to these.

[0076] The plant control composition is applied once or twice or more, but is not limited thereto. When applied twice or more, the application is performed at intervals of, but is not limited to, 3 to 15 days, 3 to 10 days, 3 to 7 days, 4 to 7 days, 5 to 7 days, or 6 days.

[0077] In one embodiment, the plant control composition is applied as a primary treatment 7 days before the pathogen invasion and as a secondary treatment 1 day before the pathogen invasion, but is not limited thereto.

[0078] Yet other aspects of the present application provide uses of amino acids or compositions comprising same for enhancing plant disease resistance, for plant control, and for preventing or treating plant diseases.

[0079] The amino acids, plant diseases, plant disease resistance, control, prevention, treatment, etc. are as described above. EXAMPLES

[0080] The present application will be described in more detail below with reference to examples. However, these examples are merely preferred embodiments illustrating the present application, and the present application is not limited thereto. Note that technical matters not described in this specification are fully understood and easily implemented by a skilled engineer in the technical field of the present application or a similar technical field. EXAMPLES

[0081] Verification of amino acid-induced resistance to plant diseases To verify the induced resistance of various amino acids to plant diseases, we used the model plant Arabidopsis thaliana and the pathogens Pseudomonas syringae pv. tomato DC3000 (hereafter referred to as Pst), which is known to cause leaf spot disease, and Pectobacterium carotovorum subsp. carotovorum SCC1 (hereafter referred to as Pcc), which is known to cause soft rot disease.

[0082] To grow the model plant, Arabidopsis seeds were surface sterilized by immersing them in 3% sodium hypochlorite (NaOCl) for 5 min. They were then rinsed five times with sterilized water and then sown on 1 / 2 Murashige and Skoog (MS) medium (0.6% agar, 1.5% sucrose, pH 5.8). After 3 days, the seeds germinated, and the germinated seeds were transferred to plates (Falcon, USA) in groups of six and cultured in a plant incubator (12 hours light and 23°C).

[0083] Two weeks after repotting, the roots of each plant were treated with 50 μl of each of the 10 amino acids at a concentration of 0.1%. Seven days after amino acid treatment, five leaves of each plant were treated with 1 × 10 8 The plants were treated with 2 μl each of cfu / ml of Pst (live parasite, Fig. 1a) and Pcc (nematode parasite, Fig. 1b) pathogens.

[0084] Prior to treatment, Pst pathogens were cultured on King's B (KB) solid medium at 30°C for 48 hours. Seven days after treatment, disease severity was observed for Pst pathogens, and the severity of disease on each leaf was scored from 0 to 5 according to the following scale, and the average was calculated. 0=no symptoms, 1=slight bleaching, 2=bleaching, 3=bleaching and small areas of necrosis, 4=necrosis, 5=extensive necrosis

[0085] Before treatment, the Pcc pathogen was cultured in Luria-Bertani broth (LB) solid medium at 30°C for 48 hours. For the plants treated with the Pcc pathogen, the disease severity of each leaf was observed 24 and 48 hours after treatment, and the degree of disease severity was rated on a scale of 0 to 5 according to the following criteria, and the average value was calculated. 0 = no symptoms, 1 = mild soft rot symptoms at the inoculation site, 2 = soft rot symptoms at the inoculation site, 3 = severe soft rot symptoms at the inoculation site, 4 = soft rot symptoms around the inoculation site, 5 = soft rot symptoms on the entire leaf

[0086] Amino acids that exerted an induced resistance effect against the disease were selected based on statistical significance compared to the water-treated control group.

[0087] As a result, it was confirmed that seven amino acids, valine, proline, isoleucine, leucine, glutamic acid, histidine, and aspartic acid, induce resistance to Pst (Figure 1a), and four amino acids, valine, proline, isoleucine, and leucine, induce resistance to Pcc (Figure 1b).

[0088] In particular, it was confirmed that valine maintained disease resistance even 48 hours after treatment with the Pcc pathogen, and that valine, proline, isoleucine, and leucine induced resistance in plants to both the live parasite Pst and the necrotic parasite Pcc. EXAMPLES

[0089] Further verification of amino acids that induce induced resistance to plant diseases Using valine, which showed the most excellent induced resistance against Pcc and Pst in Example 1, the induced resistance induction effect was confirmed again.

[0090] Valine was used at concentrations of 0.5, 0.1, 0.05, 0.01, 0.005 and 0.001%, and Pst was used as the pathogen. All other aspects were the same as in Example 1.

[0091] The selection criterion was whether or not the induced resistance to disease in the valine-treated group at each concentration was statistically different (p<0.05) from that in the water-treated group.

[0092] As a result, disease severity was generally reduced in the valine-treated group, and furthermore, the disease severity of the valine-treated groups at concentrations of 0.5% and 0.1% (0.95 mM) was 2.4 and 2.5, respectively, while the disease severity of the water-treated group reached 4.6, confirming that the disease severity of both the 0.5% and 0.1% valine-treated groups was significantly reduced. The group treated with 1 mM BTH, known as an induced resistance substance, showed a disease severity of 3.7, confirming that the 0.5% and 0.1% valine-treated groups had a better disease resistance effect than the BTH-treated group (Figure 2). EXAMPLES

[0093] Verification of induced resistance by valine and isoleucine treatment Proline and the branched chain amino acids (BCAAs) valine and isoleucine were used to test whether induction of induced resistance reduced the number of pathogens.

[0094] To this end, plants were prepared as in Example 1 and treated with amino acids (0.1% valine, 0.1% isoleucine, and 0.1% proline were selected, which were confirmed to have an induced resistance effect at low concentrations in Example 2) and pathogens.

[0095] The number of pathogenic bacteria was measured by crushing the parts of Arabidopsis plants, excluding their roots, in an e-tube containing beads, and then examining the pathogenic bacteria contained in the juice.

[0096] Arabidopsis plants were treated with Pst pathogens and the number of pathogens was measured on the 3rd and 6th days after treatment. The results showed that the number of pathogens in the 0.1% valine-treated group, the 0.1% isoleucine-treated group, and the 0.1% proline-treated group was statistically significantly reduced on the 6th day compared to the untreated control group (Figure 3a).

[0097] Arabidopsis plants were treated with Pcc pathogens and the number of pathogens was measured three days later. As a result, it was confirmed that the number of pathogens in the 0.1% valine, 0.1% isoleucine and 0.1% proline treatment groups was reduced compared to the untreated control group. The number of pathogens in the valine, isoleucine and proline treatment groups was also reduced compared to the BTH treatment group (Figure 3b). EXAMPLES

[0098] Confirmation of plant disease resistance-related gene expression responses In order to investigate whether valine, isoleucine and proline induce the expression of disease resistance-related genes, Arabidopsis thaliana (Non-Patent Document 1) containing a PR (Pathogenesis-related protein) 1a promoter GUS fusion construct, which is a disease resistance marker gene, was used. Arabidopsis thaliana plants were prepared in the same manner as in Example 1, and Arabidopsis roots were treated with 50 μl of valine, 50 μl of isoleucine and 50 μl of proline, respectively. One week after the treatment, the presence or absence of expression of the resistance marker gene PR1a was observed by GUS (β-glucuronidase; GUS reporter) activity.

[0099] Observations from 1 day after inoculation (Dai) to 4 Dai after pathogen treatment confirmed that GUS was expressed in the leaves, and PR1a was also expressed not only in the BTH-treated group, but also in the valine, isoleucine, and proline-treated groups (Figure 4). EXAMPLES

[0100] Assessment of valine- and isoleucine-induced resistance in pot experiments. Disease resistance was again examined on crops grown in pots.

[0101] The seeds of the eight crops were immersed in 3% sodium hypochlorite (NaOCl) for 5 minutes to sterilize the surface. They were then rinsed five times with sterilized water, and then sown in pots containing bed soil and cultured in a plant culture vessel (12 hours of light and 23°C temperature). They were cultivated in pots in a plant culture vessel (12 hours of light, 26°C, Vision) for 3 weeks, and then sprayed with 5 ml of each amino acid at a concentration of 0.1%. One day after amino acid treatment, the pathogens were inoculated as follows, and the control value was then calculated.

[0102] The study targeted nine pathogens on eight crops (soybean, cucumber, lettuce, rice, tomato, wheat, barley, and pepper). The pathogens targeted were as follows: Soybean stem rot (Phytophthora sojae), cucumber bacterial spot (Pseudomonas syringae pv. lachrymans), lettuce soft rot (Pectobacterium carotovorum pv. carotovora), rice blast (Magnaporthe oryzae), tomato gray mold (Botrytis cinerea), tomato stem rot (Phytophthora infestans), wheat leaf rust (Puccinia recondite), barley powdery mildew (Blumeria graminis f. sp. hordei), pepper anthracnose (Colletotrichum coccodes)

[0103] a) Soybean stem rot Phytophthora sojae, the pathogen of soybean stem rot, was inoculated into V8 medium (V8 juice, 1.8% agar) and cultured in an incubator at 25°C for 2 weeks. The spores formed were harvested in sterilized distilled water and spore suspension (5.0 × 10 5 Soybeans were inoculated with the prepared spore suspension by spraying 5 ml per plant, and the disease was allowed to develop in a constant temperature and humidity room at 26°C for 3 days, after which the disease spot area ratio (%) was investigated. The disease spot area ratio was calculated as follows:

[0104]

number

[0105] b) Cucumber bacterial spot Pseudomonas syringae pv. lachrymans, the causative agent of bacterial spot disease of cucumber, was cultured on KB (King's B) solid medium at 28°C for 48 hours. The causative agent was cultured at OD = 1 (10 9 The inoculated cucumber plants were left in a constant temperature and humidity room at 26°C for 3 days to develop the disease, and the diseased area rate (%) was investigated.

[0106] c) Lettuce soft rot Pectobacterium carotovorum pv. carotovora, the pathogen of bacterial soft rot of lettuce, was cultured in LB (Luria-Bertani broth) solid medium at 28°C for 48 hours. The pathogen reached an OD of 1 (10 9 The lettuce was treated by spraying 5 ml of the inoculated lettuce per plant at a concentration of 100 CFU / ml. The inoculated lettuce was left in a constant temperature and humidity room at 26°C for one day to develop the disease, and the disease area rate (%) was investigated.

[0107] d) Rice blast disease For rice blast disease (RCB), the pathogen Magnaporthe oryzae KI-1113a strain was inoculated onto rice bran agar medium and cultured in an incubator at 25°C for 2 weeks. The spores formed were harvested in sterilized distilled water and the spore suspension (5.0 × 10 5 Rice plants inoculated with 5 ml of the solution per plant were sprayed and left in a moist room in the dark for 24 hours, and then allowed to develop disease in a constant temperature and humidity room at 80% relative humidity and 25°C for 4 days, after which the lesion area rate (%) was investigated.

[0108] e) Tomato gray mold For tomato gray mold (TGM), the pathogenic fungus Botrytis cinerea was inoculated onto potato agar medium and incubated in an incubator at 20°C (in the dark). The spores formed were used as the inoculum. To inoculate the pathogen, the spores were harvested and the spore concentration was measured using a hemocytometer to determine the concentration of 5.0 × 10 5 The sample was diluted to spores / ml and then sprayed onto tomato seedlings (at the 2-3 leaf stage) that had been treated with the sample. The inoculated tomato seedlings were left in a moist room at 20°C (relative humidity 95% or higher) for 3 days to allow the disease to develop, and the lesion area rate (%) was investigated.

[0109] f) Tomato stem rot For tomato stem rot (TLB), the pathogenic bacterium Phytophthora infestans was inoculated into oatmeal medium and cultured in an incubator at 20°C to form zoosporangia. Sterilized distilled water was added to the medium, and the zoosporangia formed were harvested. The spore concentration was 2.0 × 10 4 A spore suspension was prepared at 100 μg / ml. This was then cooled in a refrigerator to allow the zoospores to flow out, and a zoospore suspension was prepared. This was then sprayed onto tomato seedlings (at the 2-3 leaf stage) that had been treated with the sample. The tomato seedlings inoculated with the pathogen were then kept in a moist room at 20°C for 2 days, and then allowed to develop in a constant temperature and humidity room for 2 days, after which the lesion area rate (%) was investigated.

[0110] g) Wheat leaf rust In the case of wheat leaf rust (WLR), the pathogen Puccinia recondita is a living parasite, so spores (urediniospores) formed on wheat leaves by direct subculture were used as the inoculum. For the efficacy study, five wheat seeds (Keumgang wheat) were sown in disposable pots (diameter: 4.5 cm), and the one-leaf stage seedlings grown in a greenhouse for eight days were treated with the sample, and one day later, the inoculum (0.67 g / L spores) was sprayed for inoculation. The inoculated wheat seedlings were treated in a moist room at 20°C for one day, and then transferred to a constant temperature and humidity room at 20°C with a relative humidity of 70% to induce disease development, and the lesion area rate (%) was investigated seven days after inoculation.

[0111] h) Barley powdery mildew In the case of barley powdery mildew (BPM), the pathogen Blumeria graminis f.sp.hordei is a parasitic fungus, so barley seedlings were subcultured and the spores formed on the barley leaves were used as the inoculum source. For the efficacy study, five barley seeds ("Hanyoung barley") were sown in disposable pots (diameter: 4.5 cm) and the seedlings at the one-leaf stage were grown in a greenhouse for eight days. The samples were sprayed on the seedlings and air-dried in the greenhouse. The barley treated with the samples was then inoculated by sprinkling powdery mildew spores on them. The inoculated barley seedlings were left in a constant temperature and humidity room at 20°C and a relative humidity of about 50% for seven days to allow the disease to develop, and the disease spot area rate (%) was investigated.

[0112] i) Pepper anthracnose In the case of pepper anthracnose (PAN), the pathogen Colletotrichum coccodes was inoculated into an oatmeal medium and cultured at 25°C for 10 days. The spores formed were harvested and the spore concentration was 5×10 5 A spore suspension was prepared by adjusting the concentration to spores / ml. The prepared spore suspension was sprayed onto pepper seedlings (3-4 leaf stage) treated with the sample, which were then left in a moist room (25°C) for 2 days, after which the disease was allowed to develop in a constant temperature and humidity room (25°C, 80% RH). Three days after inoculation, the area ratio (%) of lesions formed on the pepper leaves was investigated.

[0113] The control value was calculated from the lesion area rate investigated by the above methods a) to i) according to the following formula.

[0114]

number

[0115] As a result, when induced resistance was tested against nine pathogens, a control effect was confirmed compared to the untreated group against soybean stem rot (Figure 5a), cucumber bacterial spot (Figure 5b), lettuce soft rot (Figure 5c), and rice blast (Figure 5d).

[0116] Compared to the untreated control group, the control values ​​of the valine-treated group and the isoleucine-treated group were 15% and 10% for soybean stem rot, 17% and 12% for cucumber bacterial spot, 13% and 8% for lettuce soft rot, and 13% and 16% for rice blast.

[0117] In the group treated with BTH, an induced resistance inducer, the control values ​​for soybean stem rot, cucumber bacterial spot, lettuce soft rot, and rice blast were 10%, 9%, 10%, and 9%, respectively, compared to the untreated group. In addition, the control value for tomato gray mold in the BTH-treated group was 20% compared to the untreated group. In order to increase the effect of induced resistance against the pathogens to be controlled, the following experiment was conducted regarding the treatment time and method. EXAMPLES

[0118] Testing the optimum conditions for induction of induced resistance by valine and isoleucine. In order to improve the disease control value of valine and isoleucine, the induction period of induced resistance was changed. A total of three crops (soybean, cucumber, and lettuce) and three pathogens (soybean stem rot, cucumber bacterial spot, and lettuce soft rot) were targeted. Each plant was prepared in the same manner as in Example 5.

[0119] For the pathogen of soybean stem rot, disease severity was observed 7 days after the pathogen treatment and the severity was rated on a scale of 0 to 5 according to the following criteria. 0 = no symptoms, 1 = one leaf withers, 2 = two leaves withers, 3 = three leaves withers, 4 = four leaves withers, 5 = entire plant withers

[0120] For the pathogen of cucumber bacterial spot, the severity of the disease was observed 7 days after the pathogen treatment, and the degree of the disease was rated on a scale of 0 to 5 according to the following criteria. 0=no symptoms, 1=slight disease symptoms, 2=30% disease symptoms, 3=disease and small areas of necrosis, 4=necrosis, 5=large areas of necrosis and wilting symptoms.

[0121] For the pathogen of soft rot of lettuce, the severity of the disease was observed 48 hours after the treatment with the pathogen, and the severity of the disease was rated on a scale of 0 to 5 according to the following criteria. 0 = no symptoms, 1 = mild soft rot symptoms at the inoculation site, 2 = soft rot symptoms at the inoculation site, 3 = severe soft rot symptoms at the inoculation site, 4 = soft rot symptoms around the inoculation site, 5 = soft rot symptoms on the entire leaf

[0122] From the disease severity thus investigated, the control value was calculated according to the following formula.

[0123]

number

[0124] In order to obtain the optimal control value for inducing induced resistance, various amino acid treatment periods and times were tested, and it was ultimately determined that the most effective method for inducing resistance was to perform the primary amino acid treatment 7 days before pathogen treatment and the secondary treatment 1 day before.

[0125] The results when the primary amino acid treatment was performed 7 days before the pathogen treatment and the secondary treatment was performed 1 day before were as follows.

[0126] In soybean stem rot, the disease severity in the valine-treated group and the isoleucine-treated group was 1.1 and 2.3, respectively, which was reduced from 4.1 in the water control group. The calculated control values ​​were 73% and 44%, respectively, compared to the untreated group. It was confirmed that valine had a superior disease control effect to the BTH-treated group, which had a control value of 46%, and isoleucine had a disease control effect equivalent to that of the BTH-treated group (Figure 6a).

[0127] In the case of cucumber bacterial spot disease, the disease severity in the valine-treated group and the isoleucine-treated group was 2.7 and 3.6, respectively, which was reduced compared to 4.4 in the water control group. The calculated control values ​​were 39% and 18%, respectively, compared to the untreated group. On the other hand, in the BTH-treated group, it was confirmed that not only disease susceptibility but also growth was suppressed compared to the untreated group. Valine and isoleucine have a disease control effect by inducing induced resistance against cucumber bacterial spot disease, and unlike the BTH-treated group, in which growth inhibition occurred when induced resistance was induced, it was confirmed that the valine-treated group and the isoleucine-treated group did not exhibit growth inhibition (Figure 6b).

[0128] In the case of lettuce soft rot, the disease severity in the valine-treated group and the isoleucine-treated group was 2.2 and 3, respectively, which was reduced from 4.2 in the water control group. The calculated control values ​​were 48% and 29%, respectively, relative to the untreated group. The above results suggest that the valine-treated group and the isoleucine-treated group have a better disease control effect than the BTH-treated group, which had a control value of 17% (Figure 6c). EXAMPLES

[0129] Testing induced resistance using a hyperspectral camera The induced resistance to soybean stem rot was verified using a hyperspectral camera and a visible light camera. The plants were prepared in the same manner as in Example 6, and seven days after the pathogen treatment, leaves were collected from the same position in each treatment group and hyperspectral photographs were taken. The hyperspectral camera took pictures in a wavelength band of 400 to 1000 nm. The wavelength bands of normal leaves and diseased leaves are different, and this can be displayed in color. Even when it is difficult to distinguish the difference in disease severity with the naked eye, the hyperspectral camera makes it easy to distinguish.

[0130] The vegetation was quantified as the Normalized Difference Vegetation Index (NDVI). The extent of diseased leaves in the total leaf area was expressed as 0.1 to 0.9, and the number of pixels corresponding to the disease was used. Generally, a value of 0.7 or higher is considered to be a healthy leaf.

[0131] Even in the visible light range, the untreated group showed a lighter yellow-green color than the valine-treated group, and the disease progressed more rapidly (Figure 7a, RGB photograph).

[0132] When photographed with a hyperspectral camera, healthy leaves were photographed as green, dead leaves as brown, and different colors were photographed according to the severity of the disease (NDVI photo in Figure 7a). In the untreated group, healthy leaves accounted for 26%, while in the valine-treated group and isoleucine-treated group, the percentage of healthy leaves was 60% and 46%, respectively, indicating a higher percentage of healthy leaves compared to the untreated group (Figure 7b). The hyperspectral camera also reaffirmed that valine and isoleucine induce induced resistance and provide resistance to disease. EXAMPLES

[0133] Assay of the growth-promoting ability of valine The growth of soybeans and cucumbers was observed after treatment with valine. One week after sowing in pots, each plant was drenched with 5 ml of valine at a concentration of 0.1%. Each plant was drenched with valine twice at one-week intervals. Two weeks after the final treatment, the stems of each treatment group were cut and weighed.

[0134] As a result, in soybeans, the valine-treated group weighed an average of 11 g, which was 2.4 g heavier than the untreated group (8.6 g), whereas the BTH-treated group weighed 7.3 g, indicating growth inhibition (Fig. 8a).

[0135] In cucumbers, the valine-treated group weighed an average of 34g, 3g heavier than the untreated group (31g), while the BTH-treated group weighed 26g, showing growth suppression compared to the untreated group (Fig. 8b).

[0136] The above results suggest that valine also promotes the growth of soybean and cucumber.

[0137] From the above description, a person skilled in the art to which the present application pertains will understand that the present application can be implemented in other specific forms without changing its technical ideas or essential features. It should be understood that the above examples are merely illustrative and not limiting. This application should be interpreted as including all modifications or alterations derived from the meaning and scope of the claims and their equivalent concepts, rather than the specification.

Claims

1. A composition for enhancing plant disease resistance, comprising an amino acid, The amino acid is at least one selected from the group consisting of branched chain amino acids, proline, glutamic acid, aspartic acid, and histidine; The composition for enhancing resistance to a plant disease, wherein the plant disease is at least one selected from the group consisting of spot disease, bacterial soft rot, Phytophthora stem and root rot, bacterial leaf blight, and rice blast.

2. 2. The composition for enhancing plant disease resistance according to claim 1, wherein the plant disease is induced by at least one fungus selected from the group consisting of Pseudomonas syringae pv. tomato, Pectobacterium carotovorum subsp. carotovorum, Phytophthora sojae, Pseudomonas syringae pv. lachrymans, and Magnaporthe oryzae.

3. The composition for enhancing plant disease resistance according to claim 1 , wherein the branched-chain amino acid is at least one selected from the group consisting of isoleucine, leucine and valine.

4. The composition for enhancing plant disease resistance according to claim 1 , wherein the plant is at least one selected from the group consisting of beans, fruit vegetables, leafy vegetables, and grains.

5. The composition for enhancing plant disease resistance according to claim 1 , wherein the amino acid prevents or treats pathogenic infection of a plant.

6. The composition for enhancing plant disease resistance according to claim 1 , wherein the amino acid induces systemic induced resistance in a plant.

7. The composition for enhancing plant disease resistance according to claim 1 , wherein the amino acid does not inhibit plant growth.

8. 2. The composition for enhancing resistance to a plant disease according to claim 1, wherein the composition is a composition for enhancing resistance to a plant disease, the composition comprising valine, and the plant disease is at least one selected from the group consisting of leaf spot, bacterial soft rot, Phytophthora root rot, bacterial leaf blight, and rice blast.

9. 2. The composition for enhancing resistance to a plant disease according to claim 1, wherein the composition is a composition for enhancing resistance to a plant disease, the composition comprising isoleucine, and the plant disease is at least one selected from the group consisting of leaf spot, bacterial soft rot, Phytophthora root rot, bacterial leaf blight, and rice blast.

10. The composition for enhancing resistance to plant diseases according to claim 1 , wherein the composition contains leucine and is for enhancing resistance to spot disease or bacterial soft rot.

11. The composition for enhancing resistance to plant diseases according to claim 1 , wherein the composition contains proline and is for enhancing resistance to spot disease or bacterial soft rot.

12. The composition for enhancing resistance to plant disease according to claim 1 , which is for enhancing resistance to spot disease and contains at least one selected from the group consisting of glutamic acid, aspartic acid, and histidine.

13. A method for enhancing plant disease resistance, comprising the step of treating a plant body with the composition for enhancing plant disease resistance according to claim 1.

14. The method for enhancing plant disease resistance according to claim 13, wherein the plant is at least one selected from the group consisting of beans, fruit vegetables, leafy vegetables, and cereals.

15. The method for enhancing plant disease resistance according to claim 13, wherein the composition for enhancing plant disease resistance is applied twice or more.

16. The method for enhancing plant disease resistance according to claim 13, wherein the composition for enhancing plant disease resistance is applied at intervals of 3 to 15 days.

17. The method for enhancing plant disease resistance according to claim 13, wherein the method promotes plant growth.

18. A method for preventing or treating a plant disease, comprising the step of treating a plant body with the composition for enhancing plant disease resistance according to claim 1.

19. A plant control composition comprising an amino acid, The amino acid is at least one selected from the group consisting of branched chain amino acids, proline, glutamic acid, aspartic acid, and histidine; The plant disease composition is a plant control composition for controlling at least one plant disease selected from the group consisting of leaf spot, bacterial soft rot, Phytophthora stem and root rot, bacterial leaf blight, and rice blast.

20. A method for controlling plants, comprising treating a plant with the plant control composition according to claim 19.

21. 1. Use of at least one amino acid selected from the group consisting of branched chain amino acids, proline, glutamic acid, aspartic acid and histidine for enhancing plant disease resistance, comprising: The plant disease is at least one selected from the group consisting of spot disease, soft rot disease, phytophthora stem rot, bacterial spot disease and blast disease.

22. 1. Use of at least one amino acid selected from the group consisting of branched chain amino acids, proline, glutamic acid, aspartic acid and histidine for plant control, comprising: The plant disease is at least one selected from the group consisting of spot disease, soft rot disease, stem rot, bacterial spot disease and blast disease.

23. 1. Use of at least one amino acid selected from the group consisting of branched chain amino acids, proline, glutamic acid, aspartic acid and histidine for preventing or treating a plant disease, comprising: The plant disease is at least one selected from the group consisting of spot disease, soft rot disease, phytophthora stem rot, bacterial spot disease and blast disease.

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