Pyrido[1,2-a]pyrimidin-4-one derivatives

Abstract

The present invention relates to a compound represented by formula (I) TIFF2025508110000033.tif46165 (In the formula, R 1 ~R 3 and A1-A2 are as defined in the present specification and claims. The compounds of formula (I) can be used as medicines.

Description

[Technical field]

[0001] The present invention relates to novel organic compounds useful for therapy and / or prophylaxis in mammals, in particular compounds which reduce protein levels of huntingtin (HTT) and are useful in the treatment of Huntington's disease.

[0002] In particular, the present invention relates to a compound of formula (I) [ka] (In the formula, R 1 is hydrogen or alkyl, R 2 is hydrogen, halogen, alkyl, alkoxy or haloalkyl; R 3 is hydrogen, alkyl or halogen, A 1 is -N- or -C-, A 2 is -CH- or -O-, provided that A 1 If is -N-, then A 2 is -CH-, where A 1 If -C-, then A 2 is -O-), or a pharma- ceutically acceptable salt thereof. [Background technology]

[0003] Huntington's disease (HD) is an inherited autosomal dominant neurodegenerative disorder caused by a CAG repeat expansion in the huntingtin (HTT) gene. Several lines of evidence indicate that the mutant HTT gene, together with its gene product mHTT protein, contributes to HD pathogenesis through a toxic gain-of-function mechanism.

[0004] A triplet repeat expansion in exon 1 of the HTT gene is translated into polyglutamine repeats in the HTT protein that are prone to misfolding and aggregation in cells. The exact mechanism of how mutant HTT disrupts cellular function is unclear, but several processes have been implicated ranging from disruption of RNA translation, toxic RNA species, protein aggregates, RNA translation and stress granules.

[0005] At the neural circuit level, HD has been shown to affect deep brain structures such as the striatum as well as cortical regions to varying degrees. Seminal plasma mouse genetic experiments combined with human imaging experiments point to a key role of the cortico-striatal connection in HD pathogenesis (Wang et al., "Neuronal targets of mutant huntingtin genetic reduction to ameliorate Huntington's disease pathogenesis in mice," Nature medicine, 20.5(2014):536; Tabrizi et al., "Potential endpoints for clinical trials in premanifest and early Huntington's disease in the TRACK-HD study: analysis of 24 month observational data," The Lancet Neurology, 11.1(2012):42-53).

[0006] HD typically develops around 30-50 years of age, characterized by numerous symptoms across motor, cognitive and affective domains that ultimately lead to death 10-20 years after the onset of motor symptoms. CAG repeat length negatively correlates with age at onset of motor symptoms, but this only accounts for 50-70% of the variation in age at onset. To identify genetic modifiers of age at onset in HD, Lee et al. (2019, Huntington's disease onset identified by length of uninterrupted CAG, not encoded polyglutamine, and modified by DNA maintenance mechanisms. Bioarxiv doi:https: / / doi.org / 10.1101 / 529768) performed a large-scale GWAS (genome-wide association study) to uncover additional genetic modifiers of age at onset.

[0007] Various mouse models have been characterized to model aspects of HD: YAC128 mice expressing a full-length mutant HTT transgene with 128 CAG repeats, BACHD mice expressing a full-length mutant HTT genomic sequence with 97 CAG / CAA repeats, and R6 / 2 mice expressing exon 1 of a mutant human HTT gene with 110-135 CAG repeats. In addition to these mice expressing human transgenes, there is also a series of mouse models, such as the frequently used Q111, Q175 knock-in mice, in which an expanded repeat is knocked in relative to the mouse HTT locus.

[0008] There are currently no disease-modifying therapies for Huntington's disease, and several are in development. The core disease process behind the symptomatology, characterized by motor, cognitive, and behavioral symptoms, remains unmet by the various currently approved symptomatic treatments. Tetrabenazine and tiapride are currently approved for the treatment of motor symptoms, i.e., HD-associated chorea. In addition, anticonvulsants, benzodiazepines, antidepressants, and antipsychotics are also used off-label to treat motor, cognitive, and psychiatric symptoms associated with HD.

[0009] Several therapeutic strategies targeting DNA and RNA have been investigated for HTT lowering (EJWild, S.Tabrizi, Lancet Neurol.2017 16(10):837-847). HTT lowering is a promising therapeutic approach that aims to slow disease progression by reaching the root cause of Huntington's disease. HTT lowering is thought to alter if treated at the presymptomatic or symptomatic stages of disease development, thus preventing key neurodegenerative processes in the brain. However, the challenge lies in identifying patients at the correct disease stage, since the age of onset is quite variable across the population (SJTabrizi, R.Ghosh, BRLeavitt,Neuron,2019,102(4),899).

[0010] Current clinical approaches are mainly based on antisense oligonucleotides (ASOs). In addition, a few allele-specific reduction strategies, such as SNP-based ASOs and zinc finger-based gene editing approaches, are being investigated. The use of small molecules to reduce HTT expression has been hypothesized, but this strategy has not yet been tested and has not proven successful so far.

[0011] Small molecules offer the opportunity to enable HTT reduction in the brain and periphery. Furthermore, small molecule modalities allow access to patient populations that may be difficult to reach with modalities such as ASOs or gene therapy.

[0012] Thus, there is a need for new compounds that can lower mHTT. Summary of the Invention

[0013] Applicants have surprisingly found that compounds of the present invention are active in lowering mHTT and are therefore useful in the treatment of HD.

[0014] All publications, patent applications, patents, and other references mentioned herein are incorporated by reference in their entirety.

[0015] As used herein, the term "alkyl", alone or in combination, refers to a linear or branched saturated hydrocarbon group of 1 to 8 carbon atoms, particularly 1 to 6 carbon atoms, more particularly 1 to 4 carbon atoms. Examples of linear and branched C1-C8 alkyl groups are, for example, methyl, ethyl, propyl, isopropyl, butyl, isobutyl, tert-butyl, the isomeric pentyls, isomeric hexyls, isomeric heptyls, and isomeric octyls. Particular examples of "alkyl" are methyl, ethyl, and isopropyl. Methyl and ethyl are particular examples of "alkyl" in compounds of formula (I).

[0016] The terms "alkoxy" or "alkyloxy", alone or in combination, mean a radical of the formula alkyl-O-, in which the term "alkyl" has the meaning previously given. Examples of alkoxy are e.g. methoxy, ethoxy, n-propoxy, isopropoxy, n-butoxy, isobutoxy, sec-butoxy and tert-butoxy. A particular example of "alkoxy" is methoxy.

[0017] The term "oxy", alone or in combination, signifies the group --O--.

[0018] The term "halogen" or "halo", alone or in combination, means fluorine, chlorine, bromine or iodine, especially fluorine, chlorine or bromine. A preferred example of a halogen is fluorine. The term "halo", in combination with another group, unless otherwise specified, indicates substitution of said group with at least one halogen, especially 1 to 5 halogens, especially 1 to 4 halogens, i.e. 1, 2, 3 or 4 halogens.

[0019] The term "haloalkyl", alone or in combination, refers to an alkyl group substituted with at least one halogen, particularly substituted with 1 to 5 halogens, and especially substituted with 1 to 3 halogens. Particular "haloalkyl" are fluoromethyl, trifluoromethyl, difluoromethyl, fluoroethyl, fluoropropyl, and fluorobutyl. More particular "haloalkyl" are difluoromethyl and trifluoromethyl.

[0020] The term "pharmaceutically acceptable salt" refers to a salt that retains the biological effectiveness and properties of the free base or free acid and is not biologically or otherwise undesirable. Salts are formed with inorganic acids, such as hydrochloric acid, hydrobromic acid, sulfuric acid, nitric acid, phosphoric acid, especially hydrochloric acid, and organic acids, such as formic acid, acetic acid, propionic acid, glycolic acid, pyruvic acid, oxalic acid, maleic acid, malonic acid, succinic acid, fumaric acid, tartaric acid, citric acid, benzoic acid, cinnamic acid, mandelic acid, methanesulfonic acid, ethanesulfonic acid, p-toluenesulfonic acid, salicylic acid, N-acetylcysteine, and trifluoroacetic acid. In addition, these salts can be prepared by adding an inorganic or organic base to the free acid. Salts derived from inorganic bases include, but are not limited to, sodium, potassium, lithium, ammonium, calcium, and magnesium salts. Salts derived from organic bases include, but are not limited to, primary, secondary and tertiary amines, substituted amines including naturally occurring substituted amines, cyclic amines and basic ion exchange resins, such as isopropylamine, trimethylamine, diethylamine, triethylamine, tripropylamine, ethanolamine, lysine, arginine, N-ethylpiperidine, piperidine, polyamine resin salts.The compound of formula (I) can also exist in the form of zwitterions.The particularly preferred pharma-ceutically acceptable salt of the compound of formula (I) is the salt formed with trifluoroacetic acid or hydrochloric acid.

[0021] If one of the starting materials or compounds of formula (I) of the present invention contains one or more functional groups that are not stable or are reactive under the reaction conditions of one or more reaction steps, suitable protecting groups (for example those described in "Protective Groups in Organic Chemistry" by TW Greene and PG M Huts, 3rd Ed., 1999, Wiley, New York) can be introduced before the critical step applying methods well known in the art. Such protecting groups can be removed at a later stage of the synthesis using standard methods described in the literature. Examples of protecting groups are tert-butoxycarbonyl (Boc), trityl (Trt), 2,4-dimethoxybenzyl (Dmb), 9-fluorenylmethylcarbamate (Fmoc), 2-trimethylsilylethylcarbamate (Teoc), carbobenzyloxy (Cbz) and p-methoxybenzyloxycarbonyl (Moz). A particular example of a protecting group is tert-butoxycarbonyl (Boc).

[0022] Certain embodiments of the present invention relate to compounds of formula (I) as described herein, or pharma- ceutically acceptable salts thereof, in which at least one substituent comprises at least one radioisotope. Particular examples of radioisotopes include: 2 H, 3 H, 13 C. 14 C and 18 It's F.

[0023] Furthermore, the present invention includes, where applicable, all optical isomers of the compounds of formula (I), i.e. diastereomers, diastereomeric mixtures, racemic mixtures, all corresponding enantiomers and / or tautomers thereof, as well as solvates thereof.

[0024] The compounds of formula (I) may contain one or more asymmetric centers and therefore may occur as racemates, racemic mixtures, single enantiomers, diastereomeric mixtures, and individual diastereomers. Additional asymmetric centers may be present depending on the nature of the various substituents on the molecule. Each such asymmetric center independently produces two optical isomers, and all possible optical isomers and diastereomers, both as mixtures and as pure or partially purified compounds, are intended to be included in the present invention. The present invention is meant to encompass all such isomeric forms of these compounds. The independent synthesis of these diastereomers or their chromatographic separation may be achieved as known in the art by appropriate modification of the methods disclosed herein. Their absolute stereochemistry may be determined by X-ray crystallography of crystalline products or crystalline intermediates, which are derivatized, if necessary, with a reagent containing an asymmetric center of known absolute configuration. If desired, racemic mixtures of the compounds may be separated to isolate the individual enantiomers. Resolution can be carried out by methods known in the art, such as coupling a racemic mixture of a compound to an enantiomerically pure compound to form a diastereomeric mixture, followed by separation of the individual diastereomers by standard methods such as fractional recrystallization or chromatography.

[0025] The term "asymmetric carbon atom" means a carbon atom that has four different substituents. According to the Cahn-Ingold-Prelog rules, the asymmetric carbon atom can be of the "R" or "S" configuration.

[0026] The invention therefore also relates in particular to: R 1 is hydrogen or methyl; R 1 is hydrogen; R 1 is methyl; R 2 is hydrogen, alkyl, alkoxy or haloalkyl; R2 is hydrogen, methyl, methoxy or trifluoromethyl; R 2 is hydrogen; R 2 is methyl; R 2 is methoxy; R 2 is trifluoromethyl; R 3 is alkyl or halogen; R 3 is methyl or chloro; R 3 is methyl; R 3 is chloro; A1 is -N-, and A 2 is -CH-; A compound according to the invention, wherein A1 is -C- and A2 is -O-; and rac-7-(4-azaspiro[2.5]octan-7-yl)-2-(2,8-dimethylimidazo[1,2-b]pyridazin-6-yl)pyrido[1,2-a]pyrimidin-4-one, rac-7-(4-azaspiro[2.5]octan-7-yl)-2-(8-methoxy-2-methyl-imidazo[1,2-b]pyridazin-6-yl)pyrido[1,2-a]pyrimidin-4-one, rac-7-(4-azaspiro[2.5]octan-7-yl)-2-[2-methyl-8-(trifluoromethyl)imidazo[1,2-b]pyridazin-6-yl]pyrido[1,2-a]pyrimidin-4-one, rac-7-(4-azaspiro[2.5]octan-7-yl)-2-(2,7-dimethyloxazolo[5,4-b]pyridin-5-yl)pyrido[1,2-a]pyrimidin-4-one, and rac-7-(4-azaspiro[2.5]octan-7-yl)-2-(2-chloroimidazo[1,2-b]pyridazin-6-yl)pyrido[1,2-a]pyrimidin-4-one or a racemic mixture or its corresponding enantiomer, or a pharma- ceutically acceptable salt thereof.

[0027] In one embodiment of the invention, the compound of formula (I) is a compound of formula (Ia) [ka] (In the formula, R 2 is hydrogen, alkyl, alkoxy or haloalkyl; R 3 is hydrogen, alkyl or halogen); or a pharma- ceutically acceptable salt thereof.

[0028] In one embodiment of the invention, the compound of formula (I) is a compound of formula (Ib) [ka] (In the formula, R 2 is hydrogen, alkyl, alkoxy or haloalkyl, in particular alkyl; R 3 is hydrogen, alkyl or halogen, in particular alkyl; or a pharma- ceutically acceptable salt thereof.

[0029] The synthesis of compounds of formula (I) can be achieved, for example, according to the following scheme: 1 ~R 3 and A1-A2 are as defined above unless otherwise specified.

[0030] Scheme 1 [ka] The preparation of derivatives of general formula 1 can be carried out according to general scheme 1. Suzuki coupling was carried out between tert-butyl 7-oxo-4-azaspiro[2.5]octane-4-carboxylate 2 and aminopyridine compounds 3 in the presence of 4-methylbenzenesulfonohydrazide, palladium and ligand. The resulting intermediates were then easily hydrogenated with Pd / C and H2 to give derivatives 4. Condensation with malonic acid bis(2,4,6 trichlorophenyl) ester gave 2-hydroxypyrido[1,2-a]pyrimidin-4-one derivatives 5. These compounds 5 were reacted with p-toluenesulfonyl chloride to form 6. Finally, Suzuki coupling between derivatives 6 and the appropriate boronic acid / ester gave the final compounds of general formula 1 after Boc deprotection.

[0031] The present invention therefore also relates to a method for the preparation of a compound according to the invention, comprising the steps of: (a) reacting a compound represented by formula (B1) in the presence of a base and a suitable palladium catalyst in a suitable solvent [ka] and a compound of formula (B2) [ka] wherein n is 0 or 1, X is O-tosylate, O-triflate, O-mesylate or halogen, and each R in -B(OR)2 is independently selected from hydrogen and alkyl, or -B(OR)2 is an optionally substituted dioxaborolanyl, to form a compound of formula (B3): [ka] The reaction that leads to the compound (b) reacting a compound of formula (B3) in the presence of an acid in a suitable solvent to give a compound of formula (I) [ka] (Wherein, in the production method, Process R 1 , R 2 , R 3 , A1 and A2 are as defined above, and PG is a protecting group.

[0032] The reaction of step (a) may conveniently be carried out in a solvent, which may be, for example, 1,4-dioxane, acetonitrile, water or a mixture thereof.

[0033] In the reaction of step (a), the base may be, for example, K2CO3, Li2CO3, Na2CO3, KOtBu, Cs2CO3, NaOtBu or LiOtBu, in particular K2CO3.

[0034] In the reaction of step (a), the palladium catalyst can be, for example, Pd(dppf)Cl2·CH2Cl2 (0.2 equivalents CAS number 95464-05-4) or XPhos PdG4 CAS number 1599466-81-5.

[0035] In reaction step (a), X is conveniently O-tosylate or chloro, especially O-tosylate.

[0036] In reaction of step (a), B(OR)2 can be, for example, dioxaborolanyl optionally substituted with 1, 2, 3 or 4 alkyl, particularly 4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl.

[0037] Convenient conditions for the reaction of step (a) are from about 20°C to 150°C, in particular from about 40°C to 130°C, more particularly from about 60°C to 110°C, in particular about 90°C.

[0038] Particular conditions for the reaction of step (a) are using K2CO3 in 1,4-dioxane, acetonitrile, water or a mixture thereof at about 90° C. for about 2 to 8 hours.

[0039] The reaction of step (b) may conveniently be carried out in a solvent, which may be, for example, CH2Cl2 or 1,4-dioxane.

[0040] In the reaction of step (b), the acid can be, for example, TFA or HCl.

[0041] Convenient conditions for the reaction of step (b) are from about 0°C to 100°C, particularly from about 5°C to 80°C, more particularly from about 10°C to 60°C, especially from about 15°C to 40°C.

[0042] Particular conditions for the reaction of step (b) are the use of TFA in CH2Cl2 at about 15-40° C. for about 1 hour to 24 hours, particularly about 1 hour to 3 hours.

[0043] In this process the protecting group may be, for example, Boc, Trt or Dmb, especially Boc.

[0044] The present invention also relates to the compounds according to the invention when prepared according to the process of the invention.

[0045] The invention therefore also relates in particular to: A compound according to the invention for use as a therapeutically active substance; A pharmaceutical composition comprising a compound according to the invention and a therapeutically inert carrier; a compound according to the invention for use in the treatment or prevention of a neurodegenerative disease; A compound according to the invention for use in the treatment or prevention of Huntington's disease; The use of the compounds according to the invention for the treatment or prevention of neurodegenerative diseases, in particular Huntington's disease; The use of a compound according to the invention for the preparation of a medicament for the treatment or prevention of a neurodegenerative disease, in particular Huntington's disease; and A method for the treatment or prevention of a neurodegenerative disease, in particular Huntington's disease, which method comprises administering to a patient in need thereof an effective amount of a compound according to the invention.

[0046] A particular embodiment of the present invention relates to a pharmaceutical composition comprising a compound of formula (I) as described herein or a pharma- ceutically acceptable salt thereof and a pharma- ceutically acceptable auxiliary substance.

[0047] Additionally, structures depicted herein are also meant to include compounds which differ only in the presence of one or more isotopically enriched atoms. For example, compounds in which one or more hydrogen atoms are replaced with deuterium (2H), or one or more carbon atoms are replaced by a 13C- or 14C-enriched carbon are within the scope of this invention.

[0048] Furthermore, the present invention includes, where applicable, all optical isomers of the compounds of formula (I), i.e. diastereomers, diastereomeric mixtures, racemic mixtures, all corresponding enantiomers and / or tautomers thereof, as well as solvates thereof.

[0049] The compounds of formula (I) may contain one or more asymmetric centers and therefore may occur as racemates, racemic mixtures, single enantiomers, diastereomeric mixtures, and individual diastereomers. Additional asymmetric centers may be present depending on the nature of the various substituents on the molecule. Each such asymmetric center independently produces two optical isomers, and all possible optical isomers and diastereomers, both as mixtures and as pure or partially purified compounds, are intended to be included in the present invention. The present invention is meant to encompass all such isomeric forms of these compounds. The independent synthesis of these diastereomers or their chromatographic separation may be achieved as known in the art by appropriate modification of the methods disclosed herein. Their absolute stereochemistry may be determined by X-ray crystallography of crystalline products or crystalline intermediates, which are derivatized, if necessary, with a reagent containing an asymmetric center of known absolute configuration. If desired, racemic mixtures of the compounds may be separated to isolate the individual enantiomers. Resolution can be carried out by methods known in the art, such as coupling a racemic mixture of a compound to an enantiomerically pure compound to form a diastereomeric mixture, followed by separation of the individual diastereomers by standard methods such as fractional recrystallization or chromatography.

[0050] In embodiments where optically pure enantiomers are provided, optically pure enantiomer means that the compound contains greater than 90% by weight of the desired isomer, specifically greater than 95% by weight of the desired isomer, or more specifically greater than 99% by weight of the desired isomer, said weight percentages being based on the total weight of the isomers of the compound. Chirally pure or chirally enriched compounds can be prepared by chirally selective synthesis or by separation of enantiomers. Separation of enantiomers can be carried out on the final product or on a suitable intermediate.

[0051] Also, one embodiment of the present invention is a compound of formula (I) as described herein when prepared by any one of the processes described.

[0052] The compounds of formula (I) or their pharma- ceutically acceptable salts can be used as medicines (e.g., in the form of pharmaceutical preparations). The pharmaceutical preparations of the present invention can be administered internally, such as orally (e.g., in the form of tablets, coated tablets, dragees, hard and soft gelatin capsules, solutions, emulsions or suspensions), nasally (e.g., in the form of nasal sprays), rectally (e.g., in the form of suppositories), or topically to the eye (e.g., in the form of solutions, ointments, gels or water-soluble polymeric inserts). However, administration can also be carried out parenterally, such as intramuscularly, intravenously, or intraocularly (e.g., in the form of sterile injectable solutions).

[0053] The compound of formula (I) or its pharma- ceutically acceptable salt can be processed with pharma- ceutically inert, inorganic or organic adjuvants for the preparation of tablets, coated tablets, sugar-coated tablets, hard gelatin capsules, injections or external preparations.Lactose, corn starch or its derivatives, talc, stearic acid or its salts, etc., can be used as such adjuvants for tablets, doracea and hard gelatin capsules.

[0054] Suitable adjuvants for soft gelatine capsules are, for example, vegetable oils, waxes, fats, semisolid substances, and liquid polyols.

[0055] Suitable adjuvants for the production of solutions and syrups are, by way of example, water, polyols, saccharose, invert sugar, glucose etc.

[0056] Suitable adjuvants for injection solutions are, for example, water, alcohols, polyols, glycerol, vegetable oils etc.

[0057] Suitable adjuvants for suppositories are, by way of example, natural or hardened oils, waxes, fats, semisolid or liquid polyols etc.

[0058] Suitable adjuvants for topical ophthalmic formulations are, for example, cyclodextrin, mannitol or many other carriers and excipients known in the art.

[0059] In addition, the pharmaceutical preparations may contain preservatives, solubilizers, viscosity-increasing substances, stabilizers, wetting agents, emulsifiers, sweeteners, colorants, flavorings, salts for varying the osmotic pressure, buffers, masking agents, or antioxidants. The pharmaceutical preparations may also contain other therapeutically valuable substances.

[0060] The dosage can vary widely and is adapted to the individual requirements in each particular case. In general, for oral administration, a daily dosage of about 0.1 mg to 20 mg per kg of body weight, preferably about 0.5 mg to 4 mg per kg of body weight (e.g. about 300 mg per person), preferably divided into 1 to 3 individual doses, which may, if appropriate, consist of, for example, equal amounts. For topical administration, the formulation may contain 0.001% to 15% by weight of medicament, and the required dose, which may be between 0.1 and 25 mg, may be administered either by a single dose per day or per week, by multiple doses (2 to 4 times) per day, or by multiple doses per week. However, it will be clear that the upper or lower limits given herein may be exceeded, where this is indicated.

[0061] Pharmaceutical Compositions The compound of formula (I) or its pharmaceutically acceptable salts can be used as therapeutically active substances, for example in the form of pharmaceutical preparations.The pharmaceutical preparations can be administered orally, for example in the form of tablets, coated tablets, dragees, hard and soft gelatin capsules, solutions, emulsions or suspensions.However, administration can also be carried out rectally, for example in the form of suppositories, or parenterally, for example in the form of injection solutions.

[0062] The compound of formula (I) or its pharma- ceutically acceptable salt can be processed with pharma- ceutical inert inorganic or organic carriers for the manufacture of pharmaceutical preparations. Lactose, corn starch or its derivatives, talc, and stearic acid or its salts can be used as carriers for tablets, coated tablets, sugar-coated tablets, and hard gelatin capsules. Suitable carriers for soft gelatin capsules are, for example, vegetable oils, waxes, fats, semi-solid and liquid polyols, etc. However, depending on the nature of the active substance, carriers are usually not required for soft gelatin capsules. Suitable carriers for the manufacture of solutions and syrups are, for example, water, polyols, glycerol, vegetable oils, etc. Suitable carriers for suppositories are, for example, natural or hardened oils, waxes, fats, semi-liquid or liquid polyols, etc.

[0063] Furthermore, the pharmaceutical preparations may contain pharma- ceutically acceptable auxiliary substances such as preservatives, solubilizers, stabilizers, wetting agents, emulsifiers, sweeteners, colorants, flavorings, salts for varying osmotic pressure, buffers, masking agents, or antioxidants, etc. The pharmaceutical preparations of the present invention may further contain other therapeutically valuable substances.

[0064] A medicament containing a compound of formula (I) or a pharma- ceutically acceptable salt thereof and a therapeutically inert carrier is also provided by the present invention, the process for the preparation of which comprises bringing a compound of formula (I) and / or a pharma- ceutically acceptable salt thereof, and optionally one or more other therapeutically valuable substances, together with one or more therapeutically inert carriers, into a galenical dosage form.

[0065] The dosage can vary within a wide range and must be adjusted to the individual requirements in each specific case. In the case of oral administration, the dosage for adults can vary from about 0.01 mg to about 1000 mg per day of the corresponding amount of the compound of general formula (I) or its pharma-ceutically acceptable salt. The daily dosage can be administered in a single dose or in divided doses, and can also exceed the upper limit if it proves to be indicated.

[0066] The following examples are illustrative of the present invention without limiting it, but are merely representative of the present invention. Conveniently, the pharmaceutical preparation contains about 1-500 mg, in particular 1-100 mg, of a compound of formula (I). Examples of compositions according to the present invention are as follows:

[0067] Example A Tablets of the following composition are prepared in the usual manner: [Table 1]

[0068] Manufacturing procedure 1. Mix ingredients 1, 2, 3 and 4 and granulate with purified water. 2. Dry the granules at 50°C. 3. Pass the granules through suitable grinding equipment. 4. Add ingredient 5 and mix for 3 minutes; compress in a suitable press.

[0069] Example B-1 A capsule formulation is prepared having the following composition: [Table 2]

[0070] Manufacturing procedure 1. Mix ingredients 1, 2 and 3 in a suitable mixer for 30 minutes. 2. Add ingredients 4 and 5 and mix for 3 minutes. 3. Fill into suitable capsules.

[0071] The compound of formula (I), lactose and cornstarch are mixed first in a mixer and then in a pulverizer. The mixture is returned to the mixer; talc is added and mixed thoroughly. The mixture is filled by machine into a suitable capsule, for example a hard gelatin capsule.

[0072] Example B-2 Soft gelatin capsules are prepared having the following composition: [Table 3] [Table 4]

[0073] Manufacturing procedure The compound of formula (I) is dissolved in a warm melt of the other ingredients and the mixture is filled into a suitable sized soft gelatin capsule The filled soft gelatin capsule is treated according to the usual procedures.

[0074] Example C Prepare a suppository of the following composition: [Table 5]

[0075] Manufacturing procedure The suppository mass is melted in a glass or steel container, thoroughly mixed and cooled to 45°C. The finely powdered compound of formula (I) is then added thereto and stirred until completely dispersed. The mixture is poured into suitable sized suppository moulds and left to cool, then the suppositories are removed from the moulds and individually wrapped in wax paper or metal foil.

[0076] Example D An injection solution having the following composition is prepared: [Table 6]

[0077] Manufacturing procedure A compound of formula (I) is dissolved in a mixture of polyethylene glycol 400 and water for injection (partially). The pH is adjusted to 5.0 with acetic acid. The remaining amount of water is added and the volume is adjusted to 1.0 ml. The solution is filtered, filled into vials with appropriate overages, and sterilized.

[0078] Example E A sachet of the following composition is prepared: [Table 7]

[0079] Manufacturing procedure A compound of formula (I) is mixed with lactose, microcrystalline cellulose and sodium carboxymethylcellulose and granulated with a mixture of polyvinylpyrrolidone in water. The granules are mixed with magnesium stearate and flavour additives and filled into sachets. EXAMPLES

[0080] Abbreviation: AcOEt: ethyl acetate; AcOH: acetic acid; DCM: dichloromethane; DMAP: 4-dimethylaminopyridine; DMSO: dimethylsulfoxide; ES+: positive electrospray ionization; EtOAc: ethyl acetate; EtOH: ethanol; HPLC: high performance liquid chromatography; HTRF: homogeneous time resolved fluorescence; MeOH: methanol; MS: mass spectrometry; PPTS: pyridinium p-toluenesulfonate; RT: room temperature; TFA: trifluoroacetic acid.

[0081] The following examples are given to illustrate the invention and should not be considered as limiting the scope of the invention, but merely as representative thereof.

[0082] Intermediates Preparation of boronic esters or acids These derivatives were finally obtained in the form of either boronic esters, boronic acids or mixtures thereof and were used directly in the subsequent steps.

[0083] Boronic ester 1 2,8-Dimethyl-6-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)imidazo[1,2-b]pyridazine [ka] Prepared according to WO 2019 / 057740

[0084] Boronic acid ester 2 8-Methoxy-2-methyl-6-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)imidazo[1,2-b]pyridazine [ka]

[0085] Step 1: Preparation of 8-bromo-6-chloro-2-methyl-imidazo[1,2-b]pyridazine To a solution of (4-bromo-6-chloro-pyridazin-3-yl)amine (2000 mg, 9.6 mmol) and PPTS (241 mg, 0.96 mmol) in isopropanol (19 mL) was added 1-bromo-2,2-dimethoxy-propane (2.11 g, 1.56 mL, 11.5 mmol) at RT. The reaction mixture was heated to reflux for 30 h. After cooling to RT, the mixture was partitioned between ethyl acetate (50 ml) and 1M Na2CO3 sol (30 ml). The layers were separated and the organic layer was washed with 30 ml of brine, dried over sodium sulfate, filtered and concentrated in vacuo to give the crude title compound (2.37 g, 92% yield) as a light brown solid with 92% purity, which was used without further purification. MS (ES+) m / z: 246.0-248.0 [(M+H) + ]

[0086] Step 2: Preparation of 6-chloro-8-methoxy-2-methyl-imidazo[1,2-b]pyridazine: To a solution of 8-bromo-6-chloro-2-methyl-imidazo[1,2-b]pyridazine (500 mg, 2.03 mmol) and cesium carbonate (1.4 g, 4.3 mmol) in acetonitrile (10 mL) was added MeOH (400 uL, 9.89 mmol) at RT and stirring was continued for 4 h. The mixture was partitioned between ethyl acetate (30 mL) and water (30 mL). The combined organic layers were washed with 30 mL of brine, dried over sodium sulfate, filtered and concentrated in vacuo. Purification by flash afforded 6-chloro-8-methoxy-2-methyl-imidazo[1,2-b]pyridazine (337 mg, 84% yield) as a white solid. MS (ES+) m / z: 198.0 [(M+H)+]

[0087] Step 3: Preparation of 8-methoxy-2-methyl-6-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)imidazo[1,2-b]pyridazine: To a mixture of 6-chloro-8-methoxy-2-methyl-imidazo[1,2-b]pyridazine (118 mg, 0.597 mmol), bis(pinacolato)diboron (151.5 mg, 0.597 mmol, 1 equiv.) and potassium acetate (150.48 mg, 1.53 mmol) in 1,4-dioxane (1.2 mL) was added XPhos Pd G4 (22 mg, 0.026 mmol). The reaction mixture was heated at 100° C. for 1 h. The reaction mixture was cooled to RT and diluted with ethyl acetate (5-10 mL). The solids were removed by filtration. The filtrate was concentrated in vacuo to give the crude title compound, which was used directly in the next step without further purification.

[0088] Boronic acid ester 3 2-Methyl-6-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-8-(trifluoromethyl)imidazo[1,2-b]pyridazine [ka]

[0089] Step 1: Preparation of 6-chloro-2-methyl-8-(trifluoromethyl)imidazo[1,2-b]pyridazine: In a 10 mL round bottom flask equipped with a magnetic stir bar, reflux condenser and N2 inlet bubbler, [6-chloro-4-(trifluoromethyl)pyridazin-3-yl]amine (94 mg, 0.476 mmol) and pyridinium p-toluenesulfonate (11.9 mg, 0.048 mmol) were combined with isopropanol (2 mL). 1-Bromo-2,2-dimethoxy-propane (104.51 mg, 77.13 uL, 0.571 mmol, 1.2 equiv) was added and the colorless solution was stirred at 75 °C for 24 h. The resulting dark brown reaction mixture was cooled to RT, diluted with EtOAc (10 mL) and washed with saturated aqueous NaHCO3 (10 mL). The organic layer was separated, dried over sodium sulfate, filtered off and concentrated in vacuo. The crude product (120 mg of a brownish viscous oil) was purified by column chromatography to give 6-chloro-2-methyl-8-(trifluoromethyl)imidazo[1,2-b]pyridazine (46 mg, 34% yield) as a pale yellow solid. MS (ES+) m / z: 236.1 [(M+H) + ]

[0090] Step 2: Preparation of 2-methyl-6-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-8-(trifluoromethyl)imidazo[1,2-b]pyridazine: In a dry / argon flushed 20 mL microwave tube equipped with a magnetic stir bar and capseptum, 6-chloro-2-methyl-8-(trifluoromethyl)imidazo[1,2-b]pyridazine (300 mg, 1.2 mmol), bis(pinacolato)diboron (364.7 mg, 1.44 mmol) and potassium acetate (352.42 mg, 3.59 mmol) were combined with 1,4-dioxane (12 mL). The yellowish fine suspension was stirred and degassed with argon for 10-15 min before the addition of tetrakis(triphenylphosphine)palladium (69.1 mg, 0.060 mmol). The vial was sealed and stirred in a heating block (temperature: 100 °C) for 22 h. Additional tetrakis(triphenylphosphine)palladium (69 mg, 0.060 mmol) was added after 90 min, 3.5 h, and 6 h. The reaction was cooled to RT, filtered, and concentrated in vacuo. The amber viscous oil was purified by column chromatography to give 2-methyl-6-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-8-(trifluoromethyl)imidazo[1,2-b]pyridazine (428 mg, 48%) as a yellow viscous oil.

[0091] Boronic ester 4 2,7-Dimethyl-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)oxazolo[5,4-b]pyridine [ka]

[0092] Step 1: Preparation of N-(2,6-dichloro-4-methyl-3-pyridyl)acetamide: To a solution of (2,6-dichloro-4-methyl-3-pyridyl)amine (2000 mg, 11.3 mmol) and Ac2O (11.53 g, 10.66 mL, 112.98 mmol) was added a catalytic amount of DMAP. The reaction mixture was stirred at RT for 1 h and then at 60° C. overnight. The reaction mixture was cooled to RT and ethanol (10 mL, 169.46 mmol) was added slowly and stirring was continued for 30 min. The solvent was evaporated and residual AcOH was removed by azeotropic distillation with a 30 mL portion of toluene. The residue was triturated in hot ethyl acetate (15 mL). Heptane (15 mL) was added and the suspension was stirred for 1 h. The precipitate was collected by filtration, washed twice with 10 mL portions of ethyl acetate / n-heptane (1:1), and dried in vacuum to give N-(2,6-dichloro-4-methyl-3-pyridyl)acetamide (1750 mg, 71% yield) as a brown solid. MS (ES+) m / z: 219.1 [(M+H) + ]

[0093] Step 2: Preparation of 5-chloro-2,7-dimethyl-oxazolo[5,4-b]pyridine: To a solution of N-(2,6-dichloro-4-methyl-3-pyridyl)acetamide (1750 mg, 7.99 mmol) in N-methyl-2-pyrrolidinone (16 mL) was added NaH in oil (55%) (348.58 mg, 7.99 mmol) at RT. The reaction mixture was heated at 120° C. and stirred for 20 h. After cooling to RT, acetic acid (959 mg, 914 uL, 15.98 mmol) was added slowly and stirred for an additional 30 min. The mixture was partitioned between ethyl acetate (50 mL) and 1M NaHCO3 sol (20 mL). The organic layer was washed with 50 mL of water and 30 mL of brine, dried over sodium sulfate, filtered and concentrated in vacuo. Purification by flash chromatography afforded 5-chloro-2,7-dimethyl-oxazolo[5,4-b]pyridine (85 mg, 6% yield) as a white solid. MS(ES+)m / z:183.1[(M+H) + ]

[0094] Step 3: 2,7-Dimethyl-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)oxazolo[5,4-b]pyridine: The title compound was prepared from 5-chloro-2,7-dimethyl-oxazolo[5,4-b]pyridine similarly to the synthesis of boronic ester 5 in step 2.

[0095] Boronic ester 5 2-Chloro-6-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)imidazo[1,2-b]pyridazine [ka] The title compound was prepared from 2,6-dichloroimidazo[1,2-b]pyridazine similarly to the boronic ester 1.

[0096] Example 1 rac-7-(4-azaspiro[2.5]octan-7-yl)-2-(2,8-dimethylimidazo[1,2-b]pyridazin-6-yl)pyrido[1,2-a]pyrimidin-4-one [ka]

[0097] Step 1: In an argon-purged three-neck flask, 7-keto-4-azaspiro[2.5]octane-4-carboxylic acid tert-butyl ester (781 mg, 3.47 mmol, 1.2 equiv.) and 4-methylbenzenesulfonohydrazide (700 mg, 3.76 mmol, 1.3 equiv.) were dissolved in degassed 1,4-dioxane (super anhydrous) (10 mL). Then, 2-amino-5-bromopyridine (500 mg, 2.89 mmol, 1 equiv.), lithium tert-butoxide (810 mg, 10.12 mmol, 3.5 equiv.), X-PHOS (138 mg, 0.289 mmol, 0.100 equiv.) and bis(dibenzylideneacetone)palladium (83 mg, 0.145 mmol, 0.050 equiv.) were added at room temperature. The mixture was degassed with argon three times and stirred at 110° C. for 16 h. The reaction mixture was diluted with saturated NaHCO3 solution and extracted twice with EtOAc. The organic layer was washed with water and brine, dried over Na2SO4, and concentrated to dryness. The crude material was purified by flash chromatography on silica gel (40 g, AcOEt in heptane, 0 to 100%) to give tert-butyl 7-(6-amino-3-pyridyl)-4-azaspiro[2.5]oct-7-ene-4-carboxylate and / or tert-butyl 7-(6-amino-3-pyridyl)-4-azaspiro[2.5]oct-6-ene-4-carboxylate (550 mg, 62% yield) as a light brown solid. MS (ES+) m / z: 302.2 [(M+H) + ]

[0098] Step 2: The product from step 1 (550 mg, 1.82 mmol, 1 equiv) was dissolved in methanol (8 mL) and palladium on carbon (194 mg, 0.182 mmol, 0.1 equiv) was added at room temperature. The mixture was stirred vigorously under H2 for 24 h. The reaction mixture was then flushed with N2, filtered through dicalite, washed with MeOH and the solvent was concentrated to dryness. The crude material was purified by flash chromatography on silica gel (40 g, MeOH in DCM, 0 to 10%) to give rac-tert-butyl 7-(6-amino-3-pyridyl)-4-azaspiro[2.5]octane-4-carboxylate (470 mg, 85.43%) as a colorless oil. MS (ES+) m / z: 304.2 [(M+H) + ]

[0099] Step 3: rac-tert-butyl 7-(6-amino-3-pyridyl)-4-azaspiro[2.5]octane-4-carboxylate (330 mg, 1.03 mmol, 1 equiv.) was dissolved in toluene (super anhydrous) (5 mL) and malonic acid bis(2,4,6 trichlorophenyl) ester (526 mg, 1.14 mmol, 1.1 equiv.) was added at room temperature. The mixture was stirred at 80° C. for 2 h. The reaction mixture was evaporated and purified by flash chromatography (SiO2, 25 g, MeOH in DCM, 0 to 10%) to give rac-tert-butyl 7-(2-hydroxy-4-oxo-pyrido[1,2-a]pyrimidin-7-yl)-4-azaspiro[2.5]octane-4-carboxylate (280 mg, 69.3%) as a yellow solid. MS (ES+) m / z: 372.4 [(M+H) + ]

[0100] Step 4: rac-tert-Butyl 7-(6-amino-3-pyridyl)-4-azaspiro[2.5]octane-4-carboxylate (280 mg, 0.754 mmol, 1 equiv.) was dissolved in dichloromethane (super anhydrous) (5 mL) and Et3N (92 mg, 126 uL, 0.905 mmol, 1.2 equiv.) and p-toluenesulfonyl chloride γ (158 mg, 0.829 mmol, 1.1 equiv.) were added at room temperature. The mixture was stirred at room temperature for 16 hours. The reaction mixture was diluted with saturated NaHCO3 solution and extracted twice with dichloromethane. The organic layer was washed with water and brine, dried over Na2SO4 and concentrated to dryness. The crude material was purified by flash chromatography on silica gel (25 g, AcOEt in heptane, 0-100%) to give rac-tert-butyl 7-[4-oxo-2-(p-tolylsulfonyloxy)pyrido[1,2-a]pyrimidin-7-yl]-4-azaspiro[2.5]octane-4-carboxylate (325 mg, 80.4%) as a white foam. MS (ES+) m / z: 526.4 [(M+H) + ]

[0101] Step 5: In a heated, dried three-neck flask under argon was charged rac-tert-butyl 7-[4-oxo-2-(p-tolylsulfonyloxy)pyrido[1,2-a]pyrimidin-7-yl]-4-azaspiro[2.5]octane-4-carboxylate (120 mg, 0.228 mmol, 1 eq.) and potassium carbonate (69 mg, 0.502 mmol, 2.2 eq.), and a degassed solution of 0.220 M (2,8-dimethylimidazo[1,2-b]pyridazin-6-yl)boronic acid in isopropyl acetate (149 mg, 3.11 mL, 0.685 mmol, 3 eq.) and degassed water (0.5 mL) was added. The mixture was purged three times with vacuum / argon cycles and then stirred at 55° C. under argon. Palladium(II) acetate (615ug, 0.003mmol, 0.012eq) and tricyclohexylphosphine (1.5mg, 0.005mmol, 0.024eq) were combined in a separate flask flushed with argon and degassed isopropyl acetate (2mL) was added. The solution was again purged with argon and then this solution was added via syringe to the first three-neck flask at 55°C. After the addition, the reaction mixture was purged with a vacuum / argon cycle and then stirred at 75°C under argon for 1 hour. The reaction mixture was cooled to 45°C and 2ml water was added and stirred at RT for 15 minutes. The precipitate was filtered off and washed several times with water. The collected solid was purified by flash chromatography (25 g SiO2, MeOH in DCM, 0 to 10%, 20 min) to give rac-tert-butyl 7-[2-(2,8-dimethylimidazo[1,2-b pyridazin-6-yl)-4-oxo-pyrido[1,2-a]pyrimidin-7-yl]-4-azaspiro[2.5]octane-4-carboxylate (90 mg, 70.88%) as a pale yellow solid. MS (ES+) m / z: 501.5 [(M+H) + ]

[0102] Step 6: rac-tert-Butyl 7-[2-(2,8-dimethylimidazo[1,2-b]pyridazin-6-yl)-4-oxo-pyrido[1,2-a]pyrimidin-7-yl]4-azaspiro[2.5]octane-4-carboxylate (80 mg, 0.152 mmol, 1 eq.) was dissolved in ultra-dry dichloromethane (2 mL) and 4M HCl in dioxane (455 mg, 379 uL, 1.52 mmol, 10 eq.) was added. The mixture was stirred at room temperature for 2 h and then evaporated. The solid was diluted and basified (pH >10) with 1M Na2CO3 solution and extracted 3 times with DCM / MeOH 95 / 5. The organic layer was dried over Na2SO4 and concentrated to dryness. The crude material was purified by flash chromatography on silica gel (25 g, MeOH in DCM, 5 to 15%) to give rac-7-(4-azaspiro[2.5]octan-7-yl)-2-(2,8-dimethylimidazo[1,2-b]pyridazin-6-yl)pyrido[1,2-a]pyrimidin-4-one (45 mg, 72.5%) as a light brown solid. MS (ES+) m / z: 401.3 [(M+H) + ]

[0103] Example 2 rac-7-(4-azaspiro[2.5]octan-7-yl)-2-(8-methoxy-2-methyl-imidazo[1,2-b]pyridazin-6-yl)pyrido[1,2-a]pyrimidin-4-one [ka]

[0104] Step 1: In a three-neck flask, rac-tert-butyl 7-[4-oxo-2-(p-tolylsulfonyloxy)pyrido[1,2-a]pyrimidin-7-yl]-4-azaspiro[2.5]octane-4-carboxylate (80 mg, 0.152 mmol, 1 eq.), potassium carbonate (84 mg, 0.609 mmol, 4 eq.), [1,1′-bis(diphenylphosphino)ferrocene]dichloro-palladium(II) (11 mg, 0.015 mmol, 0.1 eq.) and (8-methoxy-2-methyl-imidazo[1,2-b]pyridazin-6-yl)boronic acid (79 mg, 0.381 mmol, 2.5 eq.) as previously described herein in Example 1 were dissolved in degassed acetonitrile (1.5 mL) and water (0.5 mL). After purging with argon, the mixture was stirred under argon at 100° C. for 18 h. The reaction mixture was diluted with saturated NaHCO3 solution and extracted twice with EtOAc. The organic layer was washed with water and brine, dried over Na2SO4, and concentrated to dryness. The crude material was purified by flash chromatography on silica gel (25 g, MeOH in DCM, 0 to 5%) to give rac-tert-butyl 7-[2-(8-methoxy-2-methyl-imidazo[1,2-b]pyridazin-6-yl)-4-oxo-pyrido[1,2-a]pyrimidin-7-yl]-4-azaspiro[2.5]octane-4-carboxylate (90 mg, 98.4%) as a light brown waxy solid. MS (ES+) m / z: 517.5 [(M+H) + ]

[0105] Step 2: rac-tert-butyl 7-[2-(8-methoxy-2-methyl-imidazo[1,2-b]pyridazin-6-yl)-4-oxo-pyrido[1,2-a]pyrimidin-7-yl]-4-azaspiro[2.5]octane-4-carboxylate (90 mg, 0.150 mmol, 1 eq.) was dissolved in dichloromethane (super anhydrous) (1 mL) and TFA (427 mg, 288 uL, 3.75 mmol, 25 eq.) was added. The mixture was stirred for 2 h at room temperature. The reaction mixture was concentrated, then rediluted with 2 ml of toluene and evaporated to dryness again. The brown crude material was diluted with DCM / MeOH 95 / 5 (20 mL) and water (15 mL) and neutralized by dropwise addition of 25% aqueous ammonia (1 mL). The aqueous phase (pH=12) was extracted three times with DCM / MeOH 95 / 5. The organic layers were combined, dried over Na2SO4 and concentrated to dryness. The crude material was purified by flash chromatography on silica gel (12 g, MeOH in DCM, 0 to 15%) to give rac-7-(4-azaspiro[2.5]octan-7-yl)-2-(8-methoxy-2-methyl-imidazo[1,2-b]pyridazin-6-yl)pyrido[1,2-a]pyrimidin-4-one (45 mg, 71.4%) as an off-white solid. MS (ES+) m / z: 417.3 [(M+H) + ]

[0106] Example 3 rac-7-(4-azaspiro[2.5]octan-7-yl)-2-[2-methyl-8-(trifluoromethyl)imidazo[1,2-b]pyridazin-6-yl]pyrido[1,2-a]pyrimidin-4-one [ka]

[0107] Step 1: In a three-neck flask, rac-tert-butyl 7-[4-oxo-2-(p-tolylsulfonyloxy)pyrido[1,2-a]pyrimidin-7-yl]-4-azaspiro[2.5]octane-4-carboxylate (80 mg, 0.152 mmol, 1 eq.), potassium carbonate (46 mg, 0.335 mmol, 2.2 eq.) and [2-methyl-8-(trifluoromethyl)imidazo[1,2-b]pyridazin-6-yl]boronic acid (93 mg, 0.381 mmol, 2.5 eq.) were charged and dissolved in degassed isopropyl acetate (2 mL) and water (0.5 mL). This was purged three times with vacuum / argon cycles and then stirred under argon at 55°C. Palladium(II) acetate (410ug, 0.002mmol, 0.012eq) and tricyclohexylphosphine (1.0mg, 0.004mmol, 0.024eq) were combined in another flask flushed with argon and degassed isopropyl acetate (2mL) was added. The solution was purged again with argon and then this solution was added via syringe to the first three-neck flask at 55°C. After the addition, the reaction mixture was purged with a vacuum / argon cycle and then stirred at 75°C under argon for 1 hour. The reaction mixture was cooled and quenched with 2ml water. The mixture was diluted with saturated NaHCO3 solution and AcOEt. The organic phase was separated, washed with brine, dried over Na2SO4 and evaporated. The crude material was purified by flash chromatography (25 g SiO2, MeOH in DCM, 0 to 5%) to give rac-tert-butyl 7-[2-[2-methyl-8-(trifluoromethyl)imidazo[1,2-b]pyridazin-6-yl]-4-oxo-pyrido[1,2-a]pyrimidin-7-yl]-4-azaspiro[2.5]octane-4-carboxylate (80 mg, 90%) as a yellow solid. MS (ES+) m / z: 555.4 [(M+H) + ]

[0108] Step 2: In the same manner as in Step 2 of Example 2, rac-tert-butyl 7-[2-[2-methyl-8-(trifluoromethyl)imidazo[1,2-b]pyridazin-6-yl]-4-oxo-pyrido[1,2-a]pyrimidin-7-yl]-4-azaspiro[2.5]octane-4-carboxylate was used to obtain rac-7-(4-azaspiro[2.5]octan-7-yl)-2-[2-methyl-8-(trifluoromethyl)imidazo[1,2-b]pyridazin-6-yl]pyrido[1,2-a]pyrimidin-4-one (36 mg, 61%) as a yellow solid. MS (ES+) m / z: 455.3 [(M+H) + ]

[0109] Example 4 rac-7-(4-azaspiro[2.5]octan-7-yl)-2-(2,7-dimethyloxazolo[5,4-b]pyridin-5-yl)pyrido[1,2-a]pyrimidin-4-one [ka]

[0110] Step 1: In a manner similar to step 1 of Example 2, rac-tert-butyl 7-[4-oxo-2-(p-tolylsulfonyloxy)pyrido[1,2-a]pyrimidin-7-yl]-4-azaspiro[2.5]octane-4-carboxylate and 2,7-dimethyl-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)oxazolo[5,4-b]pyridine as previously described herein in Examples 1 and 2 were used to give rac-tert-butyl 7-[2-(2,7-dimethyloxazolo[5,4-b]pyridin-5-yl)-4-oxo-pyrido[1,2-a]pyrimidin-7-yl]-4-azaspiro[2.5]octane-4-carboxylate (24 mg, 35%) as an off-white solid. MS(ES+)m / z:502.4[(M+H) + ]

[0111] Step 2: In a manner similar to step 2 of Example 2, rac-tert-butyl 7-[2-(2,7-dimethyloxazolo[5,4-b]pyridin-5-yl)-4-oxo-pyrido[1,2-a]pyrimidin-7-yl]-4-azaspiro[2.5]octane-4-carboxylate was used to obtain rac-7-(4-azaspiro[2.5]octan-7-yl)-2-(2,7-dimethyloxazolo[5,4-b]pyridin-5-yl)pyrido[1,2-a]pyrimidin-4-one (15 mg, 85%) as an off-white solid. MS(ES+)m / z:402.3[(M+H) + ]

[0112] Example 5 rac-7-(4-azaspiro[2.5]octan-7-yl)-2-(2-chloroimidazo[1,2-b]pyridazin-6-yl)pyrido[1,2-a]pyrimidin-4-one [ka]

[0113] Step 1: In a manner similar to step 1 of Example 2, rac-tert-butyl 7-[4-oxo-2-(p-tolylsulfonyloxy)pyrido[1,2-a]pyrimidin-7-yl]-4-azaspiro[2.5]octane-4-carboxylate and 2-chloro-6-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)imidazo[1,2-b]pyridazine, as previously described herein in Example 1, were used to obtain rac-tert-butyl 7-[2-(2-chloroimidazo[1,2-b]pyridazin-6-yl)-4-oxo-pyrido[1,2-a]pyrimidin-7-yl]-4-azaspiro[2.5]octane-4-carboxylate (15 mg, 17%) as a light brown solid. MS (ES+) m / z: 507.3 [(M+H) + ]

[0114] Step 2: In the same manner as in Step 2 of Example 2, rac-tert-butyl 7-[2-(2-chloroimidazo[1,2-b]pyridazin-6-yl)-4-oxo-pyrido[1,2-a]pyrimidin-7-yl]-4-azaspiro[2.5]octane-4-carboxylate was used to obtain rac-7-(4-azaspiro[2.5]octan-7-yl)-2-(2-chloroimidazo[1,2-b]pyridazin-6-yl)pyrido[1,2-a]pyrimidin-4-one (9 mg, 81%) as a yellow solid. MS(ES+)m / z:407.3[(M+H) + ]

[0115] Example 6 Homogeneous time-resolved fluorescence for lowering HTT The HTRF assay was adapted from Weiss et al. (Analytical Biochemistry Volume 395, Issue 1, December 1, 2009, Pages 8-15 and Analytical Biochemistry Volume 410, 2011, Pages 304-306) for cells from the GENEAe020-A cell line (https: / / hpscreg.eu / cell-line / GENEAe020-A).

[0116] Compounds were tested for their effect on mutant HTT levels in human cells (GENEAe020-A cell line) from Huntington's patients using homogeneous time-resolved fluorescence (HTRF) against mutant HTT protein (mHTT). The GENEAe020-A cell line was derived by Genea Biocells from human blastocysts of HD donors. After assessing viability, cells were seeded in 384-well collagen-coated plates in growth medium. Once cells had attached, the medium was removed and test compounds dissolved in DMSO were diluted in buffer and added to the attached cells. Controls included experiments without cells, DMSO without compound, and Hsp90 inhibitor controls. Cells were incubated with compounds and controls for 48 hours. Cells were then lysed and transferred to assay plates containing HTRF-labeled monoclonal antibodies developed by Paul Patterson that recognize specific regions of the HTT protein (Ko et al., Brain Research Bulletin, Volume 56, Numbers 3 and 4, 2001, Pages 319-329). A terbium-labeled "donor" antibody (2B7) binds to the N-terminus of the HTT protein, and an Alexa488-labeled "acceptor" antibody (MW1) is specific for the polyglutamine region of the protein. Binding of the acceptor-labeled antibody is more efficient for the expanded polyglutamine repeats of the mutant HTT protein, which translates into a signal boost that allows specific measurement of mutant HTT protein levels. HTRF donor and HTRF acceptor detection reagents are incubated with cell lysates, and the ratio between the signals of the two fluorophores indicates the relative amount of mHTT.

[0117] The results of the assay are shown in Table 8 below. Table 8 shows the EC for reduction of mHTT obtained for certain examples of the invention as measured by HTRF assay. 50 (Half Max Effective Concentration) values ​​are provided (data shown below are the average from three replicates). [Table 8]

Claims

1. Equation (I) 【Chemistry 1】 (In the formula, R １ is hydrogen or alkyl, R ２ These are hydrogen, halogen, alkyl, alkoxy or haloalkyl, R ３ is hydrogen, alkyl, or halogen, A １ is -N- or -C-, A ２ is -CH- or -O-, However, when A １ is -N-, A ２ is -CH-, provided that when A １ is -C-, A ２ is -O-). A compound of or a pharmaceutically acceptable salt thereof.

2. R １ The compound according to claim 1, wherein the compound is hydrogen or methyl.

3. R １ The compound according to claim 1, wherein the compound is hydrogen.

4. R ２ The compound according to claim 1, wherein the compound is hydrogen, alkyl, alkoxy, or haloalkyl.

5. R ２ The compound according to claim 1, wherein the compound is hydrogen, methyl, methoxy, or trifluoromethyl.

6. R ３ The compound according to claim 1, wherein the compound is alkyl or halogen.

7. R ３ The compound according to claim 1, wherein the compound is methyl or chloro.

8. A １ is -N-, A ２ The compound according to claim 1, wherein is -CH-.

9. A １ is -C-, A ２ The compound according to claim 1, wherein is -O-.

10. rac-7-(4-azaspiro[2.5]octan-7-yl)-2-(2,8-dimethylimidazo[1,2-b]pyridazine-6-yl)pyrido[1,2-a]pyrimidine-4-one, rac-7-(4-azaspiro[2.5]octan-7-yl)-2-(8-methoxy-2-methylimidazo[1,2-b]pyridazin-6-yl)pyrido[1,2-a]pyrimidine-4-one, rac-7-(4-azaspiro[2.5]octan-7-yl)-2-[2-methyl-8-(trifluoromethyl)imidazo[1,2-b]pyridazin-6-yl]pyrido[1,2-a]pyrimidine-4-one, rac-7-(4-azaspiro[2.5]octan-7-yl)-2-(2,7-dimethyloxazolo[5,4-b]pyridin-5-yl)pyrido[1,2-a]pyrimidine-4-one, and rac-7-(4-azaspiro[2.5]octan-7-yl)-2-(2-chloroimidazo[1,2-b]pyridazine-6-yl)pyrido[1,2-a]pyrimidine-4-one A compound according to claim 1, or a racemic mixture thereof or a corresponding enantiomer, selected from the above. Or a pharmaceutically acceptable salt thereof.

11. A method for preparing a compound according to any one of claims 1 to 10, comprising the following steps: (a) In a suitable solvent, in the presence of a base and a suitable palladium catalyst, formula (B1) 【Chemistry 2】 The compound and formula (B2) 【Transformation 3】 Reacting with the compound (wherein X is O-tosylate, O-triflate, O-mesylate, or halogen, and -B(OR)) ２ In this, each R is independently selected from hydrogen and alkyl, or -B(OR) ２ (This is an arbitrarily substituted dioxavoranil.) Formula (B3) 【Chemistry 4】 The process of obtaining the compound; (b) React the compound of formula (B3) in a suitable solvent in the presence of an acid to obtain formula (I) 【Transformation 5】 Steps to obtain the compound A method comprising at least one of the following: In this method, R １ , R ２ , R ３ A １ and A ２ A method wherein is as defined in any one of claims 1 to 9, and PG is a protecting group.

12. The compound according to any one of claims 1 to 10, for use as a therapeutically active substance.

13. A compound according to any one of claims 1 to 10, for use in the treatment or prevention of neurodegenerative diseases.

14. The compound according to claim 13, wherein the neurodegenerative disease is Huntington's disease.

15. A pharmaceutical composition comprising the compound described in any one of claims 1 to 10.

16. A pharmaceutical composition according to claim 15 for treating or preventing neurodegenerative diseases.

17. The pharmaceutical composition according to claim 16, wherein the neurodegenerative disease is Huntington's disease.

18. Use of the compound according to any one of claims 1 to 10 for preparing a medicament for treating or preventing neurodegenerative diseases.

19. The use according to claim 18, wherein the neurodegenerative disease is Huntington's disease.

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