Biocidal compositions containing isoxazoline compounds

Abstract

The present invention relates to a biocidal composition comprising component A and component B, characterized in that component A is isocycloceram and component B is a component selected from the group consisting of Bacillus genus, fermentation solids thereof, and insecticidal toxins thereof.

Description

[Technical field]

[0001] The present invention relates to compositions of biocidal active ingredients and methods of using said compositions in the agricultural field. [Background technology]

[0002] WO2011067272 discloses that certain isoxazoline compounds have biocidal activity. Summary of the Invention [Means for solving the problem]

[0003] The present invention provides a biocidal composition comprising component A and component B, wherein component A is isocycloceram and component B is a component selected from the group consisting of Bacillus sp., fermentation solids thereof, and insecticidal toxins thereof.

[0004] It has now surprisingly been found that the active ingredient compositions, i.e. active ingredient mixtures, according to the invention achieve a synergistic effect, in particular for treating lepidopteran and / or coleopteran pests, which active ingredient compositions achieve a high degree of pest control in a particularly short time. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0005] Throughout this specification, the expression "composition" refers to various mixtures or combinations of components (A) and (B), for example in a single "ready-mix" form, and in multiple spray mixtures (such as "tank mixes") consisting of separate formulations of a single active ingredient, and in combination with a single active ingredient when applied in a sequential manner, i.e., one after the other with a reasonably short interval, such as a few hours or days. The order in which components (A) and (B) are applied is not essential for the functioning of the invention.

[0006] Isocycloceram is an insecticidal pesticide with CAS number: 2061933-85-3 and has the following chemical formula:

[0007] Isocycloserum can include isomer (5S,4R), which is 4-[(5S)-5-(3,5-dichloro-4-fluoro-phenyl)-5-(trifluoromethyl)-4H-isoxazol-3-yl]-N-[(4R)-2-ethyl-3-oxo-isoxazolidin-4-yl]-2-methyl-benzamide, and can optionally include at least one isomer selected from among isomer (5S,4S), isomer (5R,4R), isomer (5R,4S), and any combination thereof. In the present invention, the isomer (5S,4S) is 4-[(5S)-5-(3,5-dichloro-4-fluoro-phenyl)-5-(trifluoromethyl)-4H-isoxazol-3-yl]-N-[(4S)-2-ethyl-3-oxo-isoxazolidin-4-yl]-2-methyl-benzamide; the isomer (5R,4R) is 4-[(5R)-5-(3,5-dichloro-4-fluoro-phenyl)-5-(trifluoromethyl)- 4H-isoxazol-3-yl]-N-[(4R)-2-ethyl-3-oxo-isoxazolidin-4-yl]-2-methyl-benzamide; the (5R,4S) isomer is 4-[(5R)-5-(3,5-dichloro-4-fluoro-phenyl)-5-(trifluoromethyl)-4H-isoxazol-3-yl]-N-[(4S)-2-ethyl-3-oxo-isoxazolidin-4-yl]-2-methyl-benzamide. When isocycloserum further comprises at least one of the isomers selected from among the isomer (5S,4S), the isomer (5R,4R), the isomer (5R,4S), and any combination thereof, the isocycloserum can comprise a molar proportion of isomer (5S,4R) of more than 50%, for example at least 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, or 99%, based on the total amount of isomers (5S,4R), (5S,4S), (5R,4R), and (5R,4S).

[0008] The molar proportion of the isomer (5S,4S) can be less than 50%, for example up to 30%, 25%, 20%, 15%, 10%, 9%, 8%, 7%, 6%, 5%, 4%, 3%, 2%, 1%, 0.5%, or 0.1%, relative to the total amount of the isomers (5S,4R), (5S,4S), (5R,4R), and (5R,4S).

[0009] The molar proportion of the isomer (5R,4R) can be less than 50%, for example up to 30%, 25%, 20%, 15%, 10%, 9%, 8%, 7%, 6%, 5%, 4%, 3%, 2%, 1%, 0.5%, or 0.1%, relative to the total amount of the isomers (5S,4R), (5S,4S), (5R,4R), and (5R,4S).

[0010] The molar proportion of the isomer (5R,4S) can be less than 50%, for example up to 30%, 25%, 20%, 15%, 10%, 9%, 8%, 7%, 6%, 5%, 4%, 3%, 2%, 1%, 0.5%, or 0.1%, relative to the total amount of the isomers (5S,4R), (5S,4S), (5R,4R), and (5R,4S).

[0011] Component B is an ingredient selected from the genus Bacillus.

[0012] The genus Bacillus is a bacterium having insecticidal and / or nematicidal activity, and examples thereof include Bacillus firmus, Bacillus cereus, Bacillus subtilis, and Bacillus thuringiensis.

[0013] The most preferred Bacillus species is Bacillus thuringiensis, more specifically Bacillus thuringiensis var. kurstaki or Bacillus thuringiensis var. tenebrionis.

[0014] For example, the Bacillus thuringiensis of the present invention may be COSTAR supplied by Syngenta, or NOVODOR supplied by Leu+Gygax or BioSciences.

[0015] In the present invention, the Bacillus composition of the present invention may be commercially available or may be obtained by culturing a Bacillus strain, such as a Bacillus thuringiensis strain, or an insecticidal mutant (strain) derived therefrom according to methods well known in the art. For example, conventional large-scale microbial culturing processes include submerged fermentation, solid-state fermentation, or liquid surface culture. Towards the end of the fermentation, as nutrients are exhausted, the cells begin to transition from the growth phase to the sporulation phase, so that the end product of the fermentation is mainly spores, metabolic products, and residual fermentation medium. Sporulation is part of the natural life cycle of Bacillus thuringiensis and is generally initiated by the cells in response to nutrient limitation. The fermentation is configured to obtain a high level of colony forming units to promote sporulation. The bacterial cells, spores and metabolites in the medium obtained by fermentation may be used directly or may be concentrated by conventional industrial methods such as centrifugation, tangential flow filtration, depth filtration and evaporation.

[0016] The term "fermentation" broadly refers to the enzymatic anaerobic or aerobical breakdown of organic material (e.g., carbon substrates) by microorganisms under controlled conditions (e.g., temperature, oxygen, pH, nutrients, etc.) to produce fermentation products. Although fermentation typically refers to processes that occur under anaerobic conditions, the term "fermentation" as used herein can also refer to processes that occur in the presence of oxygen, and as used herein, the term is not intended to be limited to strictly anaerobic conditions.

[0017] The compositions of the present invention include fermentation products. In some embodiments, the concentrated fermentation broth is washed, for example by a diafiltration process, to remove residual fermentation broth and metabolic products. The term "broth concentrate" as used herein refers to whole broth (fermentation broth) that has been concentrated by conventional industrial methods as described above, but remains in liquid form. The term "fermentation solid" as used herein refers to the solid material remaining after drying the fermentation broth. The term "fermentation product" as used herein refers to whole broth, broth concentrate, and / or fermentation solid. The compositions of the present invention include fermentation products.

[0018] In a typical embodiment, the fermentation product produced by the genus Bacillus thuringiensis also contains at least one of insecticidal toxins, such as crystal (Cry) proteins, cytolytic (Cyt) proteins, and insecticidal growth proteins (Vip). For example, Bacillus thuringiensis subsp. kurstaki strain SA-12 contains genes encoding the production of cytotoxin type K2 (CytK2), six insecticidal crystal proteins (Cry1Aa, Cry1Ab, Cry1Ac, Cry1Ia, Cry2Aa, Cry2Ab), two Cry-like proteins, and one insecticidal growth protein (VIP3Aa10).

[0019] The fermentation broth or broth concentrate can be dried using conventional drying processes or methods such as spray drying, freeze drying, tray drying, fluidized bed drying, drum drying, or evaporation, with or without the addition of a carrier.

[0020] The resulting dried product may be subjected to additional processing such as milling or granulation to achieve a particular particle size or physical form. A carrier may be added after drying.

[0021] The cell-free preparation of the fermentation broth of the strain of the invention can be obtained by any means known in the art, such as extraction, centrifugation, and / or filtration of the fermentation broth. Those skilled in the art will understand that the so-called cell-free preparation is not cell-free, but is mostly or essentially cell-free, depending on the technique used to remove the cells (e.g., centrifugation speed). The obtained cell-free preparation can be dried and / or formulated with ingredients that aid in application to plants or plant growth media. The concentration methods and drying techniques described above for the fermentation broth can also be applied to the cell-free preparation.

[0022] In one embodiment, the fermentation product is at least about 1×10 per mL of broth. 4 In another embodiment, the fermentation product comprises at least about 1 x 10 colony forming units (CFU) of a microorganism (e.g., Bacillus thuringiensis or an insecticidal variant thereof) per mL of broth. 5 In another embodiment, the fermentation product contains at least about 1 x 10 colony forming units (CFU) of microorganisms per mL of broth. 6 In yet another embodiment, the fermentation product comprises at least about 1 x 10 CFU of microorganisms per mL of broth. 7 In another embodiment, the fermentation product comprises at least about 1 x 10 CFU of microorganisms per mL of broth. 8 In another embodiment, the fermentation product comprises at least about 1 x 10 CFU of microorganisms per mL of broth. 9In another embodiment, the fermentation product comprises at least about 1 x 10 CFU of microorganisms per mL of broth. 19 In another embodiment, the fermentation product comprises at least about 1 x 10 CFU of microorganisms per mL of broth. 11 Contains CFU of microorganisms.

[0023] "Mutant" refers to a bacterial species having a modification or alteration (e.g., in a nucleotide sequence or amino acid sequence) that results in an organism and / or sequence that differs from the native or wild-type organism to which the mutant is compared. In some embodiments, the modification or alteration may be a substitution or alteration (e.g., deletion or addition) of one or more nucleotides and / or amino acids. In some embodiments, the substitution or alteration of one or more amino acids may be conservative, where such conservative amino acid substitutions and / or alterations in the "mutant" do not substantially reduce the activity of the mutant compared to the non-mutated form. In some examples, the mutation may be to render, for example, a Bacillus spp. auxotroph.

[0024] In accordance with the present invention, components (A) and (B) are present in the composition in synergistically effective amounts.

[0025] More specifically, the weight ratio in grams of the active ingredients of Component A to Component B in the composition may be in the range of 1:2000 to 1:2, preferably 1:1000 to 1:10, preferably 1:1000 to 1:100, preferably 1:400 to 1:100, more preferably 1:200 to 1:100.

[0026] Component A according to the invention can be used to prepare suspension concentrates (SC), flowable suspensions (FS), suspoemulsions (SE), suspension concentrate-capsule suspension blends (ZC), water-soluble granules (SG), water-dispersible granules (WG), water-dispersible tablets (WT), dispersible concentrates (DC) or oil dispersions (OD). The most preferred formulation types are suspension concentrates, flowable suspensions or dispersible concentrates.

[0027] The above-mentioned formulations can be prepared using ingredients and techniques well known in the art.

[0028] For example, for the preparation of the biocidal composition according to the invention, in particular component A, it may further comprise at least one component selected from among polyoxyalkylene copolymers, acrylic graft copolymers, oxygen-containing hydrocarbons, lignin compounds, lactamide compounds and ether-type solvents, preferably at least two of said components, preferably at least three of said components, more preferably at least four of said components.

[0029] In certain embodiments for forming a suspension concentrate, the preparation of the biocidal composition, in particular component A, may further comprise at least one component selected from among polyoxyalkylene copolymers, acrylic graft copolymers, and oxygen-containing hydrocarbons, preferably at least two of said components, more preferably three of said components.

[0030] In a particular embodiment for forming a dispersible concentrate, for the preparation of the biocidal composition, in particular component A, it may further comprise at least one component selected from among an acrylic graft copolymer, a lignin compound, a lactamide compound, and an ether-type solvent, preferably at least two of said components, preferably at least three of said components, more preferably four of said components.

[0031] In the present invention, the polyoxyalkylene copolymers may be derived from at least two different alkylene oxides, such as ethylene oxide and propylene oxide monomers.

[0032] The polyoxyalkylene copolymer may more preferably be a polyoxyalkylene block copolymer of the AB type, ABA type, BAB type, or ABABA type.

[0033] More specifically, the polyoxyalkylene copolymers can be prepared by the ring-opening polymerization of the corresponding cyclic ethylene oxide and propylene oxide monomers.

[0034] Typically, the ring-opening polymerization is initiated by the addition of water and an alkali hydroxide, such as sodium hydroxide and potassium hydroxide. Copolymer block structures are formed by first polymerizing a polymer block using one monomer, and then adding a second monomer to form an additional polymer block.

[0035] In a preferred embodiment, the polyoxyalkylene copolymer may be an ethylene oxide-propylene oxide-ethylene oxide block copolymer (EO-PO-EO block copolymer), i.e., a poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) block copolymer, or a poly(ethylene glycol)-poly(propylene glycol)-poly(ethylene glycol) block copolymer.

[0036] The polyoxyalkylene copolymers of the present invention, more specifically the EO-PO-EO block copolymers, may have a molecular weight of 1,000 to 15,000 g / mol, more preferably 3,000 to 7,000 g / mol.

[0037] In the present invention, the expression "molecular weight" means the average molecular weight (i.e. the approximate molecular weight). The molecular weight, i.e. the molar mass, of a polymer can be easily determined by methods well known in the art, such as gel permeation chromatography (GPC).

[0038] In the first embodiment, the EO-PO-EO block copolymer may have a polypropylene oxide molecular weight of 900-4,000 g / mol, preferably 2,000-4,000 g / mol, in other words, the polypropylene oxide molecular weight is the molecular weight of the poly(propylene oxide) block of the EO-PO-EO block copolymer.

[0039] In a second embodiment, the EO-PO-EO block copolymer may have an ethylene oxide content of 10-80%, preferably 30-50%, based on the total weight of the copolymer, in other words, the ethylene oxide content is the percentage of poly(ethylene oxide) blocks in the EO-PO-EO block copolymer.

[0040] In a third embodiment, the EO-PO-EO block copolymer can have the features of the first embodiment and the features of the second embodiment.

[0041] Examples include the GENAPOL® PF series (CLARIANT), the PLURONIC® series (BASF), the SYNPERONIC® PE series (CRODA), or the TOXIMUL® series (STEPAN).

[0042] The acrylic graft copolymer typically has a comb or star structure, preferably a comb structure.

[0043] Graft copolymers are branched copolymers in which the components forming the side chains are structurally different from the components forming the backbone.

[0044] A comb polymer consists of a main chain (backbone) that contains branch points, with linear side chains emanating from each of the branch points.

[0045] Star polymers consist of a multifunctional center with at least three polymer chains radiating from it.

[0046] In a preferred embodiment, the acrylic graft copolymer may be an amphiphilic copolymer.

[0047] More specifically, the acrylic graft copolymer comprises at least one component A (the hydrophilic portion) that is solvated by the aqueous medium, and at least one other component B that is hydrophobic.

[0048] Suitable acrylic graft copolymers can include polyethylene glycol, monomethyl ether of polyethylene glycol, poly(vinylpyrrolidone), poly(acrylamide), or poly(vinyl alcohol) as the hydrophilic side chains, and the hydrophobic backbone can include polymers and copolymers of styrene, methyl acrylate, methyl methacrylate, ethyl acrylate, 2-ethylhexyl acrylate, lauryl methacrylate, or vinyl acetate.

[0049] Such acrylic graft copolymers can be prepared, for example, by converting the monomethyl ether of polyethylene glycol to an acrylate or methacrylate ester, which is then radically polymerized with other unsaturated monomers such as styrene, ethyl acrylate, or methyl methacrylate. Such acrylic graft copolymers can also be prepared by reacting a hydrophobic polymer backbone consisting of chemically reactive sites such as carboxyl, hydroxyl, or amine groups with monomeric alkylene oxides such as ethylene oxide or propylene oxide to form hydrophilic side chains.

[0050] More preferably, the acrylic graft copolymer is a non-ionic polymer, more particularly having a comb structure.

[0051] In the present invention, the acrylic graft copolymer may contain polyethylene glycol and / or monoether polyethylene glycol side chains.

[0052] The acrylic graft copolymers may also include a backbone derived from acrylate and / or methacrylate monomers.

[0053] More preferably, the acrylic graft copolymer may comprise a backbone derived from acrylate and / or methacrylate monomers and side chains comprising polyethylene glycol and / or monoether polyethylene glycol, more particularly imparting a comb structure to the polymer.

[0054] For example, the acrylic graft copolymer of the present invention may be Atlox 4913™ supplied by CRODA or Tersperse 2500™ supplied by HUNTSMAN.

[0055] The oxygen-containing hydrocarbon compound can be selected from alkyl ether compounds, alkyl ester compounds, and any mixtures thereof, preferably from polyethylene glycol alkyl ethers, sulfonated alkyl ester compounds, and any mixtures thereof. In other words, the oxygen-containing hydrocarbon compound is preferably different from the polyoxyalkylene copolymer and the acrylic graft copolymer.

[0056] Polyethylene glycol alkyl ethers can be obtained by reacting alcohols with ethylene oxide. Suitable alcohols include those having C2-C 15 Chain length is preferably C7 to C 11 More preferably, the chain length is C9 or C 10 The alcohol may be a linear or branched aliphatic alcohol having a chain length of 0.1 to 1.0 mm. An example is Rhodasurf DA / 630-E™ supplied by SOLVAY-RHODIA.

[0057] The sulfonated alkyl ester compound may be a sulfosuccinate or a salt of a sulfosuccinate. An example is Aerosol™ OT-B supplied by SOLVAY-RHODIA.

[0058] The lignin compound may have a molecular weight of up to 10000 g / mol, preferably up to 5000 g / mol. In a preferred embodiment, the lignin compound may have a molecular weight of at least 1000 g / mol.

[0059] The lignin compound may more particularly be kraft lignin and / or non-sulfonated lignin. In a preferred embodiment, the lignin compound may be non-sulfonated kraft lignin.

[0060] Non-sulfonated Kraft lignin can be defined as lignin that has been separated from cellulose using the Kraft process (also known as the sulfuric acid process), i.e., separation using sodium hydroxide (NaOH) and sodium sulfide (NaS). Use of this process results in a non-sulfonated lignin that is distinct from lignin produced by the acidic sulfite process.

[0061] The lactamide compound can be represented by the formula I: CHCH(OH)C(=O)NR1R2(I), where R1 and R2 are each independently hydrogen or C 1-6 Alkyl, C 2-6 Alkenyl, or C 3-6 cycloalkyl, each of which is selected from the group consisting of phenyl, hydroxy, C 1-5 Alkoxy, morpholinyl, and NR3R4 (wherein R3 and R4 are each independently C 1-3 Alkyl or C 1-3 or R1 and R2 together with the nitrogen atom to which they are attached form a morpholinyl ring, a pyrrolidinyl ring, a piperidinyl ring, or an azepanyl ring, each of which is selected from C 1-3 In a preferred embodiment, R and R of the lactamide compound of formula I are each independently selected from hydrogen or C1-6 alkyl, more preferably hydrogen or C 1-3 It may be an alkyl, for example lactic acid dimethylamide or lactic acid diethylamide.

[0062] The ether type solvent can be selected from dialkyl ether, dialkylamide ether, glycol ether, aliphatic ether, and cyclic ether. For example, the ether type solvent can be methyl-5-(dimethylamino)-2-methyl-5-oxopentanoate, diethylene glycol monomethyl ether, diethylene glycol monoethyl ether, diethylene glycol monobutyl ether, diethylene glycol monophenyl ether, tetrahydrofuran, or dibutyl ether.

[0063] In the present invention, the composition may further comprise water.

[0064] The composition of the present invention may further comprise one or more formulation additives well known in the art. In particular, the formulation additives may be selected from among antifreeze agents, antifoam agents, antimicrobial agents (or biocides), viscosity modifiers (or thickeners), pH adjusters, and any mixtures thereof.

[0065] In a preferred embodiment, the composition of the present invention may further comprise at least a silicone compound as an antifoaming agent. An example of the silicone compound may include polydimethylsiloxane.

[0066] Other suitable formulation additives include antioxidants, emulsifiers, colorants (or pigments), fragrances, adjuvants, attractants, binders, buffers, solid supports (carriers), coatings, deodorants, emetics, inorganic fillers, safeners, organic solvents, light protectants, and any mixtures thereof, among others known to those skilled in the art.

[0067] The compositions of the present invention may further comprise an additional pesticide, which may be an insecticide, a fungicide, a herbicide, a synergist, a plant growth regulator, a nematicide, a plant nutrient, a plant fertilizer, and mixtures thereof.

[0068] The compositions of the present invention may contain one or more of the above components in suitable amounts, where appropriate, to obtain their respective properties.

[0069] In certain embodiments where component A is prepared to form a formulation such as a suspension concentrate, flowable suspension, suspoemulsion, suspension concentrate-capsule suspension blend, water soluble granules, water dispersible granules, water dispersible tablets, dispersible concentrates, or oil dispersions, the formulation may include: 0.01% to 50.0% by weight of component A, preferably 0.1% to 40.0% by weight of component A, based on the total weight of the formulation; a polyoxyalkylene copolymer, preferably from 0.0005% to 50.0% by weight of a polyoxyalkylene copolymer, more preferably from 0.002% to 25.0% by weight of a polyoxyalkylene copolymer, relative to the total weight of the formulation; an acrylic graft copolymer, preferably from 0.0005% to 20.0% by weight of an acrylic graft copolymer, more preferably from 0.001% to 10.0% by weight of an acrylic graft copolymer, relative to the total weight of the formulation; an oxygen-containing hydrocarbon compound, preferably from 0.0001% by weight to 10.0% by weight, more preferably from 0.001% by weight to 5.0% by weight, based on the total weight of the formulation; · Optional water.

[0070] The above formulations are preferred for forming suspension concentrates or flowable suspensions.

[0071] In certain embodiments where component A is prepared to form a formulation such as a suspension concentrate, flowable suspension, suspoemulsion, suspension concentrate-capsule suspension blend, water soluble granules, water dispersible granules, water dispersible tablets, dispersible concentrates, or oil dispersions, the formulation may include: (a) 0.01% by weight to 70.0% by weight of component A, preferably 0.1% by weight to 50.0% by weight of component A, based on the total weight of the formulation; (b) a lignin compound, preferably from 0.01% to 30.0% by weight of a lignin compound, preferably from 0.1% to 20.0% by weight of a lignin compound, based on the total weight of the formulation; (c) optionally an acrylic graft copolymer, preferably from 0.01% to 30.0% by weight of an acrylic graft copolymer, preferably from 0.1% to 20.0% by weight of an acrylic graft copolymer, based on the total weight of the formulation; (d) optionally a lactamide compound, preferably 10.0% to 90.0% by weight of a lactamide compound, preferably 20% to 80% by weight of a lactamide compound, based on the total weight of the composition; and (e) optionally an ether type solvent, preferably from 1.0% to 70.0% by weight of an ether type solvent, preferably from 1.0% to 60.0% by weight of an ether type solvent, based on the total weight of the composition.

[0072] The above formulation is preferred for forming a dispersible concentrate.

[0073] In certain of said embodiments, the isocycloceram, the polyoxyalkylene copolymer, the acrylic graft copolymer, and the oxygen-containing hydrocarbon are compounds described herein.

[0074] Component B according to the invention can be used to prepare water-dispersible granules (WG) or wettable powders (WP).

[0075] The above formulations can be prepared using ingredients and techniques well known in the art.

[0076] For example, the water-dispersible granules containing component B may be the product COSTAR® WG supplied by Syngenta.

[0077] The biocidal composition according to the invention, in particular for the preparation of component B, may further comprise at least one sulfonated compound, preferably a polyorganosulfonate, more preferably sodium poly(naphthalene formaldehyde)sulfonate.

[0078] In certain embodiments where component B is prepared to form a formulation such as a water dispersible granule or wettable powder, the formulation may include: 1.0% to 40% by weight of component B, preferably 10% to 30% by weight of component B, based on the total weight of the formulation, and 20% to 60% by weight of a sulfonated compound relative to the total weight of the formulation.

[0079] In a preferred embodiment according to the invention, the biocidal composition can be a mixture of component A and component B, more particularly the following mixture: Formulations containing component A, such as suspension concentrates, flowable suspensions, suspoemulsions, suspension concentrate-capsule suspension blends, water-soluble granules, water-dispersible granules, water-dispersible tablets, dispersible concentrates, or oil dispersions, and - Preparations containing component B such as water-dispersible granules and wettable powders.

[0080] The mixture can be diluted with water in a tank mix.

[0081] The formulation containing component A and the formulation containing component B can be added to the farm's water spray tank or may be mixed and then applied directly without further dilution.

[0082] In a further embodiment, components A and B may each be applied in any desired order or simultaneously.

[0083] In a further embodiment, the formulation containing component A and the formulation containing component B may each be applied in any desired order or simultaneously.

[0084] Another object of the invention relates to a method for combating and / or controlling pests, which comprises applying to the pests, to their habitat, to plants susceptible to attack by pests or to plant propagation material susceptible to attack by pests a combination of components A and B, characterized in that components A and B are as defined in the present invention.

[0085] In other words, the combination is a biocide combination comprising component A and component B as defined in the present invention, where component A is isocycloceram and component B is a component selected from the genus Bacillus, its fermentation solids, and its insecticidal toxins. The combination can be applied in any desired order or simultaneously.

[0086] The combination of components A and B may be a biocidal composition according to the invention.

[0087] In the method according to the present invention, the composition can typically utilize an effective amount of Isocycloceram and an effective amount of Bacillus sp. More specifically, the weight ratio of component A to component B in the composition can range from 1:1 to 1:10000, preferably 1:1 to 1:2000, preferably 1:1 to 1:1000, preferably 1:1 to 1:500, preferably 1:1 to 1:400, preferably 1:1 to 1:300, more preferably 1:1 to 1:200, more preferably 1:1 to 1:100, more preferably 1:1 to 1:10, more preferably 1:2 to 1:10.

[0088] More specifically, for foliar application, the weight ratio of component A to component B in the composition may be in the range of from 1:100 to 1:120, preferably from 1:110 to 1:115, for example 1:112.5.

[0089] For seed treatment applications, the weight ratio of component A to component B in the composition may range from 1:2 to 1:20, preferably from 1:3 to 1:10, more preferably from 1:5 to 1:10.

[0090] The composition may be a mixture of component A (i.e., a formulation containing component A) according to the present invention and component B (i.e., a formulation containing component B) according to the present invention. Water may optionally be added to the mixture.

[0091] The pests may be invertebrate pests such as insects and mites. More preferably, the pests may be selected from the orders Lepidoptera and Coleoptera.

[0092] The Lepidoptera may be of the Noctuidae family (e.g., Spodoptera sp., Helicoverpa sp., Trichoplusia sp., Chrysodeixis sp., Autographa sp., etc.) and Gelechiidae family (e.g., Tuta sp., Anarsia sp., Phthorimaea sp., etc.). Most preferred pests may be of the Spodoptera genus (e.g., Spodoptera littoralis, etc.), Tuta genus (e.g., Tuta absoluta, etc.), or Helicoverpa genus (e.g., Helicoverpa armigera, etc.).

[0093] The order Coleoptera includes the family Elateridae (e.g. Agriotes spp., Ludius spp., Ctenicera spp., Selatosomus spp., Chyptohypnus spp., Hypnoidus spp., Limonius spp., Aeolus spp., Hadromorphus spp., Hypnoidus spp., Melanotus spp., Pheletes spp., Conoderus spp., Athous spp., and the genera Aeolus spp., ... spp., Agrypnus spp., Agrypnus variabilis, Pheltes californicus, Ludius aeripennis tinctus, Ludius aeripennis destructor, Ctenicera destructor, Ctenicera aeripennis destructor, Selatosomus destructor, Selatosomus aeripennis destructor, Selatosomus aeripennis aeripennis aeripennis, Chyptohypnus nocturnus bicolor, Cryptohypnus nocturnus(Eschscholtz) , Hypnoidus nocturnus, Hypolithus bicolor, Limonius californicus, Aeolusmellillus, Agriotes mancus, Hadromorphus glaucus, Agriotes brevis, Agriotes rufipalpis, Agriotes proximus, Agriotes littigiosus, Agriotes criddlei, Agriotes lineatus, Agriotes obscurus, Agriotes sputator, Agriotes sordidus, Agriotes ustulatus ustulatus, Hypnoidus abbreviates, Limonius canus (Limonius canus, Limonius pctoralis, Limonius infuscatus, Agriotes fuscicollis, Melanotus opacaicicollis leconte, etc.), Tenebrionidae (e.g. Gonocephalum spp., Adelium spp., Pterohelaeus spp., Gonocephalum aequatriale, etc.),aequatoriale, G. bilineatum, G. carpentariae (northern wireworm), G. contractum, G. depressum, G. dorsogranosum, G. elderi (plant beetle), G. hoffmannseggii, G. macleaya, G. misellum, G. patruele, G. pusillum, G. reticulatum, G. rusticum, G. seriatum, G. setulosum, G. simplex (dusty surface beetle), G. torridum, G. tuberculatum, G. walker, Adelium brevicorne, Pterohelaeus alternatus, etc.), Chrysomelidae (e.g. Diabrotica spp., Leptinotarsa ​​spp., Phyllotreta spp., Chaetocnema spp., etc.), Scarabaeidae (e.g. Phyllophaga spp., Cyclocephala spp., Popilia spp., Halotrichia spp., etc.), spp.), Curculionidae (e.g. Anthonomus spp., Sphenopherus spp., Otiorhynchus spp.), and Nitidulidae (e.g. Meligethes spp.). Most preferred pests are, for example, Diabrotica balteaata.The species may be of the genus Diabrotica, for example Agriotes balteata, or of the genus Agriotes, for example Agriotes lineatus.

[0094] The compositions according to the invention can be used to combat and / or control (i.e. contain or destroy) pests of the above-mentioned types occurring in plants, especially useful and ornamental plants in agriculture, horticulture and forestry, or in organs such as fruits, flowers, leaves, stems, tubers or roots of such plants, and in some cases even subsequently formed plant organs remain protected from these pests. The compositions can also be used to combat and / or control (i.e. contain or destroy) pests of the above-mentioned types occurring in particular in the soil and / or on plant propagation material.

[0095] The term "plants" as used herein includes seedlings, shrubs, and trees. Suitable target crops include, inter alia, cereals such as wheat, barley, rye, oats, rice, corn (e.g., fodder corn, popcorn, maize), millet, or sorghum; beets, such as sugar beet or fodder beet; fruits, such as pome fruits, stone fruits, or soft fruits, such as apples, pears, plums, peaches, almonds, cherries, or berries, such as strawberries, raspberries, or blackberries; legumes, such as beans, lentils, peas, or soybeans; rapeseed, mustard, poppy, olives, sunflowers, coconuts, castor, cocoa, or other crops. oil crops such as peanuts; cucurbits such as pumpkin, cucumber, melon, watermelon, or squash; fiber plants such as cotton, flax, hemp, or jute; citrus fruits such as oranges, lemons, grapefruit, or mandarins; vegetables such as spinach, lettuce, asparagus, cabbage, broccoli, cauliflower, carrots, onions, tomatoes, potatoes, eggplant, bell peppers, or paprika; Lauraceae plants such as avocado, Cinnamomum, or Camphor; and tobacco, nuts, coffee, sugar cane, tea, grapes, hops, Plantago, and latex plants.

[0096] In a preferred embodiment, the plant may be selected from: · Cereals such as wheat, barley, rye, oats, rice, maize (e.g. feed maize, popcorn, corn), millet or sorghum; · Legume crops such as beans, lentils, peas, or soybeans; Fiber plants such as cotton, flax, hemp or jute; and Vegetables such as spinach, lettuce, asparagus, cabbage, broccoli, cauliflower, carrots, onions, tomatoes, potatoes, bell peppers, or paprika.

[0097] The term "plant propagation material" is understood to refer to all reproductive parts of a plant, such as seeds, that can be used for plant propagation, including vegetative plant material, such as cuttings. Plant propagation material can include seeds (in the strict sense), roots, fruits, tubers, bulbs, rhizomes, plant parts. The plant propagation material can be treated with the composition of the invention before sowing or planting the material. Alternatively, the plant propagation material can be treated with the composition of the invention during sowing or planting. Furthermore, the composition of the invention can be applied to previously treated propagation material before or during planting. The composition of the invention can be applied during sowing of seeds. The composition can also be used on plant propagation material from plants grown in greenhouses and / or on plant propagation material during transplanting.

[0098] More preferably, the plant propagation material is a plant seed. The seed treatment can be performed on unsown seeds, and the term "unsown seeds" is intended to include seeds at any time from harvesting the seeds to sowing the seeds in the ground for plant germination and growth. Treatment on unsown seeds is not intended to include the application of the composition to the soil, but is intended to include any application action that targets the seeds during the sowing / planting process. The treated plant propagation material of the present invention can be treated in the same manner as conventional plant propagation material. The treated propagation material can be stored, handled, sown, and cultivated in the same manner as other biocide-treated materials.

[0099] In a first particular embodiment, the biocidal composition according to the invention can be applied to pests, their habitats, or plants susceptible to pest attack, for example in a spray of the biocidal composition dispensed from a spray container for foliar application. The method can include an effective amount of the biocidal composition applied in the following ratio: from 0.01 g to 200 g of component A per hectare, preferably from 0.1 g to 100 g of component A per hectare, more preferably from 50 to 100 g of component A per hectare; and 0.1g to 1000g of component B per hectare, preferably 1g to 500g of component B per hectare, more preferably 100g to 500g of component B per hectare.

[0100] For foliar application, more particularly, the weight percentage of component B is at least 10 times the weight percentage of component A, preferably at least 50 times the weight percentage of component A, more preferably at least 100 times the weight percentage of component A. Preferably, the weight percentage of component B is at most 200 times the weight percentage of component A. In a second particular embodiment, the biocidal composition according to the invention may be applied to plant propagation material susceptible to attack by pests. In this case, the biocidal composition may be used as a seed treatment. The method may comprise applying an effective amount of the biocidal composition in the following rates: 0.01 g to 30 g of component A per 100 kg of seeds, preferably 0.01 g to 25 g of component A per 100 kg of seeds, preferably 0.1 g to 25 g of component A per 100 kg of seeds, preferably 0.1 g to 10 g of component A per 100 kg of seeds, preferably 0.1 g to 5 g of component A per 100 kg of seeds, more preferably 0.5 g to 1 g of component A per 100 kg of seeds, and 1g to 1000g of component B per 100kg of seeds, preferably 5g to 500g of component B per 100kg of seeds, preferably 10g to 200g of component B per 100kg of seeds, preferably 10g to 150g of component B per 100kg of seeds, more preferably 50g to 100g of component B per 100kg of seeds.

[0101] More specifically for seed treatment applications, the weight rate of component B is at least 2 times the weight rate of component A, preferably at least 2.5 times the weight rate of component A, preferably at least 3 times the weight rate of component A, more preferably 5 times the weight rate of component A, more preferably 10 times the weight rate of component A. The weight rate of component B can be up to 50 times the weight rate of component A, preferably up to 40 times the weight rate of component A, more preferably up to 30 times the weight rate of component A.

[0102] In another object, the biocidal composition of the present invention can be used for the destruction and / or control of the following pests: from the order Lepidoptera, more preferably from the family Noctuidae (e.g. Spodoptera sp., Helicoverpa sp., Trichoplusia sp., Chrysodeixis sp., Autographa sp., etc.) or the family Gelechiidae (e.g. Tuta sp., Anarsia sp., Phthorimaea sp., etc.); and / or the order Coleoptera, more preferably the family Elateridae (e.g. Agriotes spp., Ludius spp., Ctenicera spp., Selatosomus spp., Chyptohypnus spp., Hypnoidus spp., Limonius spp., Aeolus spp., Hadromorphus spp., Hypnoidus spp., Melanotus spp., Pheletes spp., Conoderus spp., Athous spp., spp., Agrypnus spp., Agrypnus variabilis, Pheletes californicus, Ludius aeripennis tinctus, Ludius aeripennis destructor, Ctenicera destructor, Ctenicera aeripennis destructor, Selatosomus destructor, Selatosomus aeripennis destructor, Selatosomus aeripennis aeripennis aeripennis, Chyptohypnus nocturnus bicolor, Cryptohypnus nocturnus(Eschscholtz), Hypnoidus nocturnus, Hypolithus bicolor, Limonius californicuscalifornicus, Aeolus mellillus, Agriotes mancus, Hadromorphus glaucus, Agriotes brevis, Agriotes rufipalpis, Agriotes proximus, Agriotes littigiosus, Agriotes criddlei, Agriotes lineatus, Agriotes obscurus, Agriotes sputator sputator, Agriotes sordidus, Agriotes ustulatus, Hypnoidus abbreviates, Limonius canus (Limonius pctoralis, Limonius infuscatus, Agriotes fuscicollis, Melanotus opacaicicollis leconte, etc.), Tenebrionidae (e.g. Gonocephalum spp., Adelium spp., Pterohelaeus spp., etc.), spp.), Gonocephalum aequatoriale (Gonocephalumaequatoriale, G. bilineatum, G. carpentariae (northern wireworm), G. contractum, G. depressum, G. dorsogranosum, G. elderi (plant beetle), G. hoffmannseggii, G. macleaya, G. misellum, G. patruele, G. pusillum, G. reticulatum, G. rusticum, G. seriatum, G. setulosum, G. simplex (dusty surface beetle), G. torridum, G. tuberculatum, G. walker, Adelium brevicorne, Pterohelaeus alternatus, etc.), Chrysomelidae (e.g. Diabrotica spp., Leptinotarsa ​​spp., Phyllotreta spp., Chaetocnema spp., etc.), Scarabaeidae (e.g. Phyllophaga spp., Cyclocephala spp., Popilia spp., Halotrichia spp., etc.), spp.), Curculionidae (e.g. Anthonomus spp., Sphenopherus spp., Otiorhynchus spp.), and Nitidulidae (e.g. Meligethes spp.). Most preferred pests are, for example, Diabrotica balteaata.The species may be of the genus Diabrotica, for example Agriotes balteata, or of the genus Agriotes, for example Agriotes lineatus.

[0103] The active ingredient compositions according to the invention in particular achieve a high degree of pest control within a very short time, for example within at least 3 days after application.

[0104] In the present invention, further active ingredients can be used in the method of the present invention in combination with component A and / or component B of the present invention, and can be applied simultaneously or sequentially with components A and B of the present invention. When applied simultaneously, these further active ingredients can be formulated together with the composition of the present invention or can be mixed, for example, in a spray tank. These further biocidal active ingredients can be fungicides, herbicides, insecticides, bactericides, acaricides, nematicides, plant growth regulators, and / or biological agents.

[0105] The following mixtures of components A and / or B with further active ingredients are preferred: acibenzolar-s-methyl, metalaxyl-M, metalaxyl, picarbutrazox, oxathiapiproline, ethaboxam, fludioxonil, azoxystrobin, thiabendazole, difenoconazole, tebuconazole, ipconazole, mefentrifluconazole, prothioconazole, triticonazole, sedaxane, impirfluxam, penflurane, fluopyram, fluxapyroxad, pydiflumetofen, cilobutrifluram, thiamethoxam, isoflucipyram, clothianidin, benzovindiflupyr, cymoxanil, phosphites (phosphonates), silthiofam, pyraclostrobin, isotianil, tolprocarb, quinofumelin, Ipflufenoquine, Methyltetraprole, Florylpicosamide, Fenpicosamide, Fluoxythioconazole, Fluindapyr, Isoflucipram, Fluoxapiproline, Broflanilide, Imidacloprid, Acetamiprid, Cyantraniliprole, Chlorantraniliprole, Tetraniliprole, Fluxamethamide, Penthiopyrad, Mandipropamide, Zinpropyridaz, Flupirimine, Triflumezopyrim, Oxazosulfil, Diclobenthiazox, Benzpyrimoxane, Hymexazole, Pyrapropoin, Cyclaniliprole, Mandestrobin, Copper, Lambda-Cyhalothrin, Fenamacril, Abamectin, Tefluthrin, Picoxystrobin, Spiropydione, Oligosaccharin, Tea Tree (Melaleuca alternifolia), fosthiazate, fenamiphos, oxamyl, fluensulfone, fluazaindolizine, spirotetramat, spidoxamat, fluazinam, indazapiroxameth, thiophanate methyl, zoxamide, fluoxastrobin, pirimiphos, tetrachlorantraniliprole, bifenthrin, and emamectin.

[0106] The following mixtures of component A and / or component B with further active ingredients are most preferred: acibenzolar-s-methyl, metalaxyl-M, metalaxyl, picarbutrazox, oxathiapiproline, ethaboxam, fludioxonil, azoxystrobin, thiabendazole, difenoconazole, tebuconazole, ipconazole, mefentrifluconazole, prothioconazole, triticonazole, sedaxane, impilfluxam, penflurane, fluopyram, fluxapyroxad, pydiflumetofen, cylobutrifluram, thiamethoxam.

[0107] In certain embodiments, component A and / or component B may be combined with at least two, three, or four of the additional active ingredients mentioned above.

[0108] More specifically, component A and / or component B may be combined with at least two of the further active ingredients selected from sedaxane, mefenoxam, difenoconazole, thiamethoxam and fludioxonil, preferably with at least three of the further active ingredients selected from sedaxane, mefenoxam, difenoconazole, thiamethoxam and fludioxonil, preferably with at least four of the further active ingredients selected from sedaxane, mefenoxam, difenoconazole, thiamethoxam and fludioxonil, more preferably with all of said further active ingredients selected from sedaxane, mefenoxam, difenoconazole, thiamethoxam and fludioxonil. A preferred combination may be a combination of component A with sedaxane, mefenoxam, difenoconazole, and thiamethoxam, or a combination of component A with sedaxane, mefenoxam, difenoconazole, and fludioxonil.

[0109] When combining component A with component B with at least one of the further active ingredients, or when combining component A with at least two of the further active ingredients, or when combining component B with at least two of the further active ingredients, the weight ratio between any two components, independently of each other, may be 1:100 to 100:1, preferably 1:50 to 50:1, more preferably 1:10 to 10:1. For example, the mixture may be: component A (7.7 g / L), sedaxane (7.7 g / L), mefenoxam (15.3 g / L), difenoconazole (36.8 g / L), thiamethoxam (61.3 g / L); component A (19.1 g / L), sedaxane (19.1 g / L), mefenoxam (38.4 g / L), difenoconazole (92.2 g / L), and thiamethoxam (153.4 g / L); or component A (15.4 g / L), sedaxane (15.4 g / L), mefenoxam (9.2 g / L), difenoconazole (36.9 g / L), and fludioxonil (7.7 g / L). EXAMPLES

[0110] The following non-limiting examples demonstrate the improved performance associated with biocidal compositions according to the present invention.

[0111] A synergistic effect exists whenever the effect of a combination of active ingredients is greater than the sum of the effects of the individual ingredients.

[0112] The expected effect E of a given combination of active ingredients can be calculated according to the so-called COLBY formula as follows (COLBY, SR “Calculating synergistic and antagonistic responses of herbicide combination”. Weeds, Vol. 15, pages 20-22; 1967): ppm = milligrams of active ingredient per liter of spray mixture X=% effect of active ingredient A) using p ppm of active ingredient Y=% effect of active ingredient B) using q ppm of active ingredient. According to COLBY, the expected (additive) effect of active ingredients A) + B) using p+q ppm of active ingredients is:

[0113] If the actual observed action (O) is greater than the expected action (E), the action of the combination is superadditive, i.e. synergy exists. In mathematical terms, the synergy coefficient SF corresponds to O / E. In agricultural practice, an SF of 1.2 or more indicates a significant improvement over the addition of purely complementary actions (expected activity), while an SF of 0.9 or less in practical application routines indicates a reduced activity compared to the expected activity.

[0114] The products used in the following examples are detailed below: · VIRANTRA SC400 (commercialized by Syngenta) is an SC400 formulation (suspension concentrate containing 400 g of active ingredient per liter) containing isocycloceram as the active ingredient; · FS400 is a free-flowing concentrate for seed treatment containing 400 g of active ingredient per litre, with isocycloceram as the active ingredient; COSTAR WG18 (commercialized by Syngenta) is a WG product (water-dispersible granules containing 18% active ingredient by weight of the total product) containing 90.4 million IU / g of Bacillus thuringiensis var. kurstaki strain SA-12 (Bt1) as active ingredient; NOVODOR FC is a SC product (suspension concentrate containing 30 g / L of active ingredient) containing 10,000 I.U. / mg of Bacillus thuringiensis var. tenebrionis strain NB-176 (Bt2) as the active ingredient.

[0115] Experiment description Example 1 (Spodoptera sp.) The products VIRANTRA SC400 and COSTAR WG18 were diluted with water to the concentrations of active ingredient summarized in Tables 1 and 2 and sprayed (500 l / ha) on soybean plants (planted in pots, approximately 8 plants per pot, 5 pots per treatment) using a turntable sprayer. After drying, 10 soybean plants were cut and placed in Petri dishes (diameter: 14.5 cm) containing 3 sheets of moist filter paper (4 ml water). Three replicates were performed for each concentration. The plants were infested with 10 larvae (2nd instar larvae) of the Egyptian armyworm (Spodoptera littoralis) and the Petri dishes were covered with a round cotton cloth and closed with a plastic lid. Mortality was evaluated 3 and 5 days after spraying.

[0116] The results are summarized in Tables 1 and 2 below, where Table 1 relates to the results 3 days after application (3DAA) and Table 2 relates to the results 5 days after application (5DAA).

[0117] [Table 1]

[0118] [Table 2]

[0119] Example 2 (Diabrotica sp.) The products FS400 and NOVODOR 3% FC were diluted by adding water to the active ingredient dose for 30 seeds + 10% excess. This 10% excess was needed to compensate for the loss of active ingredient during the seed treatment process. 30 maize seeds were placed in a round glass beaker and the application solution was added. The beaker was shaken to mix the seeds and the solution. After drying, 4 seeds were sown per pot. Five replicates per treatment were prepared. 14 days after sowing, the pots were infested with five 2nd instar larvae of Diabrotica balteata. Larval mortality and plant damage were evaluated 6 days after infestation (6DAI).

[0120] Table 3 relates to the results of mortality assessment 6 days after infestation, and Table 4 relates to the plant damage assessment 6 days after infestation (6DAI).

[0121] [Table 3]

[0122] [Table 4]

[0123] Example 3 (Agriotes sp.) The products FS400 and NOVODOR 3% FC were diluted by adding water to the active ingredient dose for 30 seeds + 10% excess. This 10% excess was needed to compensate for the loss of active ingredient during the seed treatment process. 30 barley seeds were placed in a round glass beaker and the application solution was added. The beaker was shaken to mix the seeds and the solution. After drying, 2 seeds were sown per pot. 10 replicates per treatment were prepared. 5 days after sowing, the pots were infested with 10 wireworms (Agriotes lineatus, body length approx. 20 mm). 20 days after infestation (20DAI), the corrected mortality of the wireworms was evaluated based on the control treatment. 6 days after infestation (6DAI), the height of the plants was measured.

[0124] The results are summarized in the tables below, where Table 5 relates to the results of the mortality assessment 20 days after infection (20DAI) and Table 6 relates to the plant damage (plant height, cm) assessment 6 days after infection (6DAI).

[0125] [Table 5]

[0126] [Table 6]

Claims

1. A biocidal composition comprising component A and component B, wherein component A is isocycloceram, and component B is a component selected from the genus Bacillus, its fermented solids, and its insecticidal toxins.

2. The biocidal composition according to claim 1, characterized in that component B is Bacillus thuringiensis, preferably Bacillus thuringiensis var. kurstaki or Bacillus thuringiensis var. tenebrionis.

3. The biocidal composition according to claim 1, characterized in that the weight ratio of component A to component B may be in the range of 1:1 to 1:10000, preferably 1:1 to 1:2000, preferably 1:1 to 1:1000, and more preferably 1:1 to 1:

500.

4. The biocidal composition according to claim 1, characterized in that component A is used to prepare a suspension concentrate, a fluid suspension, a suspension emulsion, a suspension concentrate-capsule suspension blend, water-soluble granules, water-dispersible granules, water-dispersible tablets, a dispersible concentrate, or an oil dispersion.

5. The biocidal composition according to claim 1, characterized in that component B is used to prepare water-dispersible granules or a wettable powder.

6. The biocidal composition according to claim 1, further comprising at least one component selected from polyoxyalkylene copolymers, acrylic graft copolymers, oxygen-containing hydrocarbons, lignin compounds, lactamide compounds, and ether-type solvents.

7. The biocidal composition according to claim 1, further comprising at least one sulfonated compound, preferably a polyorganosulfonate, and more preferably sodium poly(naphthaleneformaldehyde)sulfonate.

8. The biocidal composition according to claim 1, further comprising one or more additives selected from an antifreeze, an antifoaming agent, an antimicrobial agent (or biocide), a viscosity modifier, a pH adjuster, and any mixture thereof.

9. A method for exterminating and / or controlling harmful animals, comprising applying a combination of components A and B to harmful organisms, habitats of harmful organisms, plants susceptible to attack by harmful organisms, or plant propagation materials susceptible to attack by harmful organisms, characterized in that components A and B are as defined in any one of claims 1 to 8.

10. The method according to claim 9, characterized in that the components A and B are administered by mixing a preparation containing component A with a preparation containing component B.

11. The method according to claim 9, characterized in that the biocide composition is applied by spraying.

12. The method according to claim 9, wherein components A and B are applied in a sequential manner.

13. The method according to claim 9, characterized in that the aforementioned harmful organism belongs to the order Lepidoptera and / or Coleoptera.

14. The method according to claim 9, characterized in that the plant is selected from cereals, leguminous crops, fiber plants, and vegetables.

15. Use of the biocidal composition according to any one of claims 1 to 8 for the extermination and / or control of lepidopteran and / or coleopteran pests.