Method for producing hypochlorous acid

Abstract

The present disclosure provides compositions, products, kits, and methods for producing hypochlorous acid solutions as needed. The present disclosure provides stable dry-kneaded products, kits, or packages that can be used by end-users to easily prepare hypochlorous acid compositions having desired characteristics and functionality. Hypochlorous acid is a non-toxic disinfectant, sanitizer, and decontaminant. It is naturally produced by the human immune system to fight infections. Neutrophils, a type of white blood cell, produce hypochlorous acid to kill bacteria, bacterial spores, viruses, and fungi.

Description

Technical Field

[0001] Cross - Reference to Related Applications This application claims the benefit of U.S. Provisional Patent Application No. 63 / 341,214, filed May 12, 2022, the entire disclosure of which is incorporated herein by reference.

[0002] This application relates to the production of hypochlorous acid and its compositions, as well as products and kits designed for end - users to produce and use hypochlorous acid solutions.

Background Art

[0003] Hypochlorous acid is a non - toxic disinfectant, sanitizer, and decontaminant. It is naturally produced by the human immune system to fight infections. Neutrophils, a type of white blood cell, produce hypochlorous acid to kill bacteria, bacterial spores, viruses, and fungi.

[0004] Although hypochlorous acid is efficiently and effectively produced by the human immune system, attempts to manufacture commercially available hypochlorous acid have mainly been disappointing because hypochlorous acid is unstable and has a short shelf life. Due to this short shelf life, attempts to produce stable hypochlorous acid continue. Most attempts to produce stable hypochlorous acid have used either (1) the electrolysis of metal (e.g., sodium, calcium, lithium, potassium) chloride solutions or (2) the acidification of metal hypochlorite solutions (e.g., U.S. Published Application No. 2015 / 0119245). For example, attempts have been made to produce hypochlorous acid by the electrolysis of brine (NaCl) solutions. However, this manufacturing process requires dedicated equipment and is costly, which is a problem. Due to many advantages associated with the use of hypochlorous acid solutions for sterilization, disinfection, and removal of contaminants, as well as other drawbacks, there remains a long - standing unmet need for hypochlorous acid products, kits, or packages that are available to consumers for the preparation and use of hypochlorous acid solutions in the field. Further, heretofore, it has been necessary to create a disinfecting solution (HOCl) that can be manufactured from dry - blend components. The present disclosure provides stable dry - blend products, kits, or packages that can be used by end - users to easily prepare hypochlorous acid compositions having desired characteristics and functionality.

Prior Art Documents

Patent Documents

[0005]

Patent Document 1

Summary of the Invention

Means for Solving the Problems

[0006] The present disclosure provides products, kits, and processes for creating a disinfecting solution (HOCl) manufactured from dry - blend components. The present disclosure provides products, kits, and methods having the following characteristics: capable of rapidly generating large quantities of hypochlorous acid solution as needed, the delivery method of which can be selected as directed by operational requirements (e.g., sprayer, spray, spray bottle), the products and kits can be safely used at the proposed concentrations without the need for protective gear such as goggles, gloves, or aprons, the dry - mixed chemical products have a long shelf life and can be stored until needed, the manufacturing cost is very low, the packaging size and configuration of the dry - blend can be easily changed to meet the production needs of end - users, water can be used from a source within the facility (e.g., tap water), so the transportation cost and logistics cost are low.

[0007] In a first aspect of the present disclosure, the kit comprises: a) a pre-measured amount of hypochlorite, such as calcium hypochlorite; b) a pre-measured mixture of a weakly acidic cation exchange resin and a strongly acidic cation exchange resin; and c) instructions for preparing hypochlorous acid, wherein components a) and b) are individually packaged.

[0008] In some aspects, the solution obtained according to the instructions for preparing the hypochlorous acid solution has a free available chlorine concentration of about 7000 to about 700 ppm. In some aspects, the obtained solution has a free available chlorine concentration of about 7000 to about 4000 ppm. Solutions with a free available chlorine concentration level of about 7000 to about 4000 ppm are useful when it is more difficult to kill microorganisms and / or when it is only possible to apply the solution to the surface when the contact time is short. Suitable applications include treating surfaces that may be contaminated by Bacillus anthracis (anthrax) or Clostridioides difficile (c. difficile). In some aspects,

[0009] In some aspects, the solution obtained according to the instructions for preparing the hypochlorous acid solution has a free available chlorine concentration of about 1000 to about 700 ppm. Hypochlorous acid solutions with a free available chlorine concentration of about 1000 to about 700 ppm are suitable for general surface decontamination or disinfection.

[0010] In some aspects, the solution obtained according to the instructions for preparing the hypochlorous acid solution has a free available chlorine concentration of about 700 ppm to about 50 ppm. In one aspect, the obtained solution has a free available chlorine concentration of about 200 to about 50 ppm. Solutions with a free available chlorine concentration level of about 200 to about 50 ppm are particularly suitable for treating mammalian skin or mammalian wounds, including human wounds.

[0011] In some aspects, the hypochlorous acid solution has a pH of about 4 to about 7.

[0012] In some embodiments, the amount of calcium hypochlorite measured in advance is an amount that provides from about 1.7 g / L to about 1.8 g / L with respect to a predetermined amount of water.

[0013] In some embodiments, the mixture of the weakly acidic cation exchange resin and the strongly acidic cation exchange resin measured in advance is included in an amount that provides from 6 g / L to about 20 g / L of the weakly acidic cation exchange resin with respect to a predetermined amount of water, and in an amount that provides from about 6 g / L to about 12 g / L of the strongly acidic cation exchange resin with respect to a predetermined amount of water.

[0014] In some embodiments, the mixture of the weakly acidic cation exchange resin and the strongly acidic cation exchange resin measured in advance is included in an amount equal to about 10 g of the weakly acidic cation exchange resin per 1 L of water based on a predetermined amount of water, and in an amount equal to about 8 g of the strongly acidic cation exchange resin per L based on a predetermined amount of water.

[0015] In some embodiments, the amount of calcium hypochlorite measured in advance is an amount from about 0.1 g / L to about 0.2 g / L with respect to a predetermined amount of water.

[0016] In some embodiments, the mixture of the weakly acidic cation exchange resin and the strongly acidic cation exchange resin measured in advance is included in an amount that provides from 0.3 g / L to about 2.3 g / L of the weakly acidic cation exchange resin with respect to a predetermined amount of water, and in an amount that provides from about 0.3 g / L to about 1.4 g / L of the strongly acidic cation exchange resin with respect to a predetermined amount of water.

[0017] In some embodiments, the mixture of the weakly acidic cation exchange resin and the strongly acidic cation exchange resin measured in advance is included in an amount equal to about 1.1 g of the weakly acidic cation exchange resin per 1 L of water based on a predetermined amount of water, and in an amount equal to about 0.9 g of the strongly acidic cation exchange resin per L based on a predetermined amount of water.

[0018] In some embodiments, the weakly acidic cation exchange resin is in the hydrogen form of the weakly acidic cation exchange resin and contains a carboxylic acid functional group, and the strongly acidic cation exchange resin is in the hydrogen form of the strongly acidic cation exchange resin and contains a sulfonic acid functional group.

[0019] In some embodiments, the kit further comprises d) a pre-measured amount of a surfactant.

[0020] In some embodiments, each of component a), component b), and optionally component d) is packaged in a separate airtight and watertight container.

[0021] In some embodiments, the pre-measured amount of surfactant, once prepared, is an amount necessary to constitute from 0.1 wt% to 5 wt% of the hypochlorous acid solution.

[0022] In some embodiments, the pre-measured amount of surfactant, once prepared, is an amount necessary to constitute from 0.5 wt% to 1 wt% of the hypochlorous acid solution.

[0023] In some embodiments, the surfactant is selected from the group consisting of dioctyl sulfosuccinate, alkyldiphenyloxide disulfonate, polysorbate, ethoxylated polyoxypropylene block copolymer, alcohol ethoxylate, sodium polyacrylate polymer, sodium lauriminodipropionate, sodium octyliminodipropionate, and combinations thereof.

[0024] In some embodiments, components a), b), and optionally d) are individually packaged within a three-layer bag comprising oriented polypropylene, metallized polyethylene terephthalate, and linear low-density polyethylene.

[0025] In some embodiments, the instructions for preparing the hypochlorous acid solution state that component a), component b), optionally component d), and a predetermined amount of water are to be mixed.

[0026] In some embodiments, when preparing the hypochlorous acid solution according to the instructions included in the kit, the prepared hypochlorous acid solution is further diluted with water.

[0027] In some embodiments, a pre-measured amount of calcium hypochlorite and a mixture of weakly acidic cation exchange resin and strongly acidic cation exchange resin are present in an amount effective to produce hypochlorous acid in an amount sufficient to reduce the measured concentration of microorganisms by 99%, for example, 99.9% when in continuous contact with a surface for about 30 seconds to about 2 minutes. In certain embodiments, the amount of calcium hypochlorite and the mixture of weakly acidic cation exchange resin and strongly acidic cation exchange resin are present in an amount effective to produce hypochlorous acid in an amount sufficient to kill 99.99% of the bacteria on the surface. In the products, kits, and processes of the present disclosure, a pre-measured amount of calcium hypochlorite and a mixture of weakly acidic cation exchange resin and strongly acidic cation exchange resin are present in an amount effective to produce hypochlorous acid in an amount sufficient to kill bacteria, viruses, and fungi to a level of 99.9% of the original number of bacteria, viruses, or fungi on a specific surface area of an inanimate, plant, or animal surface when in continuous contact with the surface for about 30 seconds to about 2 minutes. The components can be included in an amount sufficient to provide a hypochlorous acid solution capable of disinfecting a surface area of about 10 square feet to about 20,000 square feet.

[0028] In another embodiment of the present disclosure, hypochlorous acid is prepared by dissolving a pre-measured amount of calcium hypochlorite in water, adding a pre-measured amount of a mixture of weakly acidic cation exchange resin and strongly acidic cation exchange resin to the water, and stirring, wherein the pre-measured amount of calcium hypochlorite and the mixture and water are in an amount effective to produce a hypochlorous acid solution having a pH of about 4 to about 7 at the end of stirring.

[0029] In some embodiments, the free available chlorine concentration of the hypochlorous acid solution is from about 7000 ppm to about 50 ppm.

[0030] In some embodiments, the free available chlorine concentration of the hypochlorous acid solution is from about 7000 ppm to about 4000 ppm.

[0031] In some embodiments, the free available chlorine concentration of the hypochlorous acid solution is from about 4000 ppm to about 700 ppm.

[0032] In some embodiments, the free available chlorine concentration of the hypochlorous acid solution is from about 1000 ppm to about 700 ppm.

[0033] In some embodiments, the free available chlorine concentration of the hypochlorous acid solution is from about 1000 ppm to about 200 ppm.

[0034] In some embodiments, the free available chlorine concentration of the hypochlorous acid solution is from about 200 ppm to about 50 ppm.

[0035] In some embodiments, the free available chlorine concentration of the hypochlorous acid solution remains at about 1000 - 700 ppm when stored at room temperature for up to 48 hours.

[0036] In some embodiments, the free available chlorine concentration of the hypochlorous acid solution remains at about 200 ppm - 50 ppm when stored at room temperature for up to 48 hours.

[0037] In some embodiments, the amount of pre - measured calcium hypochlorite is in an amount of about 1.7 g / L to about 1.8 g / L relative to a predetermined amount of water.

[0038] In some embodiments, a pre - measured mixture of weakly acidic cation exchange resin and strongly acidic cation exchange resin is included in an amount that provides about 6 g / L to about 20 g / L of weakly acidic cation exchange resin relative to a predetermined amount of water, and in an amount that provides about 6 g / L to about 12 g / L of strongly acidic cation exchange resin relative to a predetermined amount of water.

[0039] In some embodiments, a pre - measured mixture of weakly acidic cation exchange resin and strongly acidic cation exchange resin is included in an amount equal to 10 g of weakly acidic cation exchange resin per 1 L of water based on a predetermined amount of water, and in an amount equal to about 8 g of strongly acidic cation exchange resin per L based on a predetermined amount of water.

[0040] In some embodiments, the amount of pre - measured calcium hypochlorite is in an amount of about 0.1 g / L to about 0.2 g / L relative to a predetermined amount of water.

[0041] In some embodiments, a mixture of pre-measured weakly acidic cation exchange resin and strongly acidic cation exchange resin is included in an amount that provides from 0.3 g / L to about 2.3 g / L of the weakly acidic cation exchange resin per a predetermined amount of water, and in an amount that provides from about 0.3 g / L to about 1.4 g / L of the strongly acidic cation exchange resin per a predetermined amount of water.

[0042] In some embodiments, a mixture of pre-measured weakly acidic cation exchange resin and strongly acidic cation exchange resin is included in an amount equal to about 1.1 g of the weakly acidic cation exchange resin per 1 L of water and in an amount equal to about 0.9 g of the strongly acidic cation exchange resin per L based on a predetermined amount of water.

[0043] In some embodiments, the process further includes adding a pre-measured amount of a surfactant while stirring.

[0044] In some embodiments, the pre-measured amount of the surfactant, once prepared, is an amount necessary to constitute from 0.1 wt% to 5 wt% of the hypochlorous acid solution.

[0045] In some embodiments, the pre-measured amount of the surfactant, once prepared, is an amount necessary to constitute from 0.5 wt% to 1 wt% of the hypochlorous acid solution.

[0046] In some embodiments, the surfactant is selected from the group consisting of dioctyl sulfosuccinate, alkyldiphenyloxide disulfonate, polysorbate, ethoxylated polyoxypropylene block copolymer, alcohol ethoxylate, sodium polyacrylate polymer, sodium lauriminodipropionate, sodium octyliminodipropionate, and combinations thereof.

[0047] In some embodiments, the solution is stirred for from about 3 minutes to about 5 minutes.

[0048] In some embodiments, the solution is stirred for about 4 minutes.

[0049] In some embodiments, the weakly acidic cation exchange resin is in the hydrogen form of the weakly acidic cation exchange resin and contains a carboxylic acid functional group, and the strongly acidic cation exchange resin is in the hydrogen form of the strongly acidic cation exchange resin and contains a sulfonic acid functional group.

[0050] In some embodiments, calcium hypochlorite, a mixture of a weakly acidic cation exchange resin and a strongly acidic cation exchange resin, and optionally a surfactant are poured and mixed from individual packages for a) calcium hypochlorite and b) the mixture of the weakly acidic cation exchange resin and the strongly acidic cation exchange resin.

[0051] In some embodiments, calcium hypochlorite is packaged in a first three-layer bag, the mixture of the weakly acidic cation exchange resin and the strongly acidic cation exchange resin is packaged in a second three-layer bag, and optionally the surfactant is packaged in a third three-layer bag, and the calcium hypochlorite and the resin mixture, and optionally the surfactant, are mixed with a predetermined amount of water.

[0052] In some embodiments, the hypochlorous acid solution is further diluted with water.

[0053] In some embodiments, a pre-measured amount of calcium hypochlorite and a mixture of weakly acidic cation exchange resin and strongly acidic cation exchange resin are present in an amount effective to produce hypochlorous acid in an amount sufficient to reduce the measured concentration of microorganisms by 99%, for example, 99.9% when continued to contact the surface for about 30 seconds to about 2 minutes. In certain embodiments, the amount of calcium hypochlorite and the mixture of weakly acidic cation exchange resin and strongly acidic cation exchange resin are present in an amount effective to produce hypochlorous acid in an amount sufficient to kill 99.99% of the bacteria on the surface. In the products, kits, and processes of the present disclosure, a pre-measured amount of calcium hypochlorite and a mixture of weakly acidic cation exchange resin and strongly acidic cation exchange resin are present in an amount effective to produce hypochlorous acid in an amount sufficient to kill bacteria, viruses, and fungi to a level of 99.9% of the original number of bacteria, viruses, or fungi on a specific surface area of inanimate, plant, or animal surfaces when continued to contact the surface for about 30 seconds to about 2 minutes. The components can be included in an amount sufficient to provide a hypochlorous acid solution capable of disinfecting a surface area of about 10 square feet to about 20,000 square feet.

Brief Description of the Drawings

[0054]

Figure 1

Modes for Carrying Out the Invention

[0055] The present disclosure relates to methods and compositions for producing a hypochlorous acid solution as needed.

[0056] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this disclosure belongs. In case of conflict, the present application, including definitions, will control. Further, unless the context clearly requires otherwise, singular terms shall include pluralities and plural terms shall include the singular. All publications, patents, and other references mentioned herein are incorporated herein by reference in their entirety for all purposes as if each individual publication or patent application were specifically and individually indicated to be incorporated by reference.

[0057] Although methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present disclosure, suitable methods and materials are described below. The materials, methods, and examples are illustrative only and not intended to be limiting. Other features and advantages of the present disclosure will become apparent from the detailed description and from the claims.

[0058] I. Definitions To further understand the present disclosure, the following terms and definitions are provided.

[0059] Unless the context clearly dictates otherwise, the singular forms “a,” “an,” and “the” include plural referents. The terms “a” (or “an”), “one or more,” and “at least one” are used interchangeably herein. In certain embodiments, the term “a” or “an” means “one.” In other embodiments, the term “a” or “an” includes “two or more” or “plural.”

[0060] The term “about” is used herein to mean approximately, roughly, nearly, or almost. When the term “about” is used in conjunction with a numerical range, this term modifies the range by extending the boundaries to include the numbers recited before and after the given number. In general, the term “about” is used herein to modify a numerical value by a width of plus or minus (±) 10% of the recited value.

[0061] Throughout the present disclosure, various aspects are presented in a range format. A numerical range includes the numerical values that define the range. When a range of values is recited, each integer value intervening between the upper and lower values recited for that range, as well as each fractional part thereof, is to be clearly understood to be disclosed, along with each sub-range between such values. It is to be understood that the upper and lower limits of any range can independently be included in or excluded from the range, and that each range in which either the upper or lower limit is included, each range in which neither is included, or each range in which both are included, is also encompassed by the present disclosure. Accordingly, the ranges recited herein are to be understood to be all shorthand representations of values within the ranges including the recited endpoints. For example, the range of 1 to 10 is understood to include any number, combination of numbers, or sub-range from the group consisting of 1, 2, 3, 4, 5, 6, 7, 8, 9, and 10.

[0062] When values are explicitly recited, it is to be understood that values that are substantially the same in magnitude or amount as the recited values are also within the scope of the present disclosure. When a certain combination is disclosed, each partial combination of the elements of that combination is also specifically disclosed and is included within the scope of the present disclosure. Conversely, when different elements or groups of elements are disclosed individually, their combinations are also disclosed. When any element of the present disclosure is disclosed as having a plurality of alternatives, examples of the disclosure in which each alternative is excluded, either alone or in any combination with other alternatives, are also disclosed herein, two or more elements of the disclosure may have such exclusions, and all combinations of elements having such exclusions are disclosed herein.

[0063] As used herein, the term "and / or" should be regarded as each specific disclosure with or without one of two designated features or components. Thus, the term "and / or" as used in phrases such as "A and / or B" herein is intended to include "A and B", "A or B", "A" (alone), and "B" (alone). Similarly, the term "and / or" as used in statements such as "A, B, and / or C" is intended to include each of the following aspects: A, B, and C; A, B, or C; A or C; A or B; B or C; A and C; A and B; B and C; A (alone); B (alone); and C (alone).

[0064] It is understood that when an aspect is disclosed herein using the language "comprising", similar aspects are also provided with respect to "consisting of" and / or "consisting essentially of" separately disclosed.

[0065] II. Hypochlorites In some aspects of the present disclosure, a stable composition of hypochlorous acid can be produced using a hypochlorite as a starting material. In some aspects, the hypochlorite is sodium hypochlorite, calcium hypochlorite, lithium hypochlorite, or potassium hypochlorite. In some aspects, the hypochlorite is calcium hypochlorite.

[0066] Calcium hypochlorite has the molecular formula of Ca(ClO) 2 and a molecular weight of 142.974 g / mol. Its CAS number is 7778-54-3. Its specific gravity is 1.21 g / cm 3 .

[0067] Calcium hypochlorite is commercially available. Methods for producing calcium hypochlorite are well known in the art. For example, calcium hypochlorite can be produced by reacting calcium hydroxide with chlorine gas as follows. 2Cl 2 +2Ca(OH) 2 →Ca(OCl)2 +CaCl 2 +2H 2 O.

[0068] III. Strongly acidic and weakly acidic cation exchange resins Ion exchange resins are composed of a polymeric matrix to which ionic "functional groups" are permanently attached, either positively charged ions (cations) or negatively charged ions (anions). Some special resins have both types of functionality. These functional groups have a net negative or positive charge, which allows the functional groups to readily attract counterions, or ions of the opposite charge. When a liquid stream flows through an ion exchange resin, the counterions can be replaced by ions of the same charge.

[0069] Broadly speaking, resins are named according to the type of ions they exchange, meaning that cationic resins exchange positively charged ions and anionic resins exchange negatively charged ions. The types of ion exchange resins include strongly acidic cation resins, weakly acidic cation resins, strongly basic anion resins, weakly basic anion resins, and special resins.

[0070] Strongly acidic cation (SAC) exchange resins are composed of a polymeric matrix to which anionic functional groups such as sulfonate (SO 3- ) are attached. The resin typically derives its exchange activity from the sulfonic functional group. Strongly acidic cation resins are commercially available from multiple sources. Mitsubishi offers SAC resins in the DIAION series. Millipore offers SAC resins in the AMBERLITE, DOWEX 50, and AMERLYST series. Purolite and ResinTech also offer SAC resins. In some embodiments, the SAC exchange resin is ResinTech CG10-H.

[0071] Weak acid cation (WAC) exchange resins are composed of a polymer matrix to which carboxylic acid functional groups such as carboxylic acid (COOH) are bonded. Weak acid cation resins are commercially available from multiple sources. Mitsubishi offers SAC resins in the DIAION series. DuPont offers AmberLite MAC-3. Purolite and ResinTech also offer WAC resins. In some embodiments, the WAC exchange resin is ResinTech WACG-HP. In some embodiments, the WAC exchange resin is ResinTech WACMP. In some embodiments, the WAC exchange resin is AmberLite MAC-3.

[0072] IV. Method for producing hypochlorous acid Certain embodiments of the present disclosure relate to a process for producing hypochlorous acid.

[0073] The principle of generation for producing a hypochlorous acid (HOCl) solution from the dry-blended components of the present disclosure is simple - the pH of the calcium hypochlorite bleach solution is lowered from 11.0 to 7.0, and HOCl is generated via ion exchange. Weak acid cation (WAC) exchange resins and strong acid cation (SAC) exchange resins are polymer resins in which hydrogen ions are bonded within the polymer network. The hydrogen ions are released from the polymer network when cations with a higher affinity for the resin reach the ion exchange sites. In the case of the dry-mixed disinfection system of the present disclosure, the chemical formula Ca(OCl) 2Calcium hypochlorite having [it] is mixed with water, and a calcium hypochlorite bleaching solution with a pH of about 11 is produced. The concentration is controlled by the ratio of calcium hypochlorite to the water used. To produce the exchange resins of HOCl, WAC, and SAC, it is mixed with the calcium hypochlorite solution at a carefully set mixing ratio. At this point, ion exchange occurs, and calcium ions are exchanged with the hydrogen ions bound to the resin. This brings about the conversion of the solution from a calcium hypochlorite-based bleaching agent to hypochlorous acid. Since the weakly acidic cation exchange resin has a much higher affinity for calcium ions than for hydrogen ions, this conversion occurs rapidly. By using a combination of the WAC exchange resin and the SAC exchange resin, a reaction solution and a hypochlorous acid solution are surely produced within an appropriate pH range, and a controlled reaction system having sufficient stability and safety for the production and use by end-users is provided. The ion exchange process serves to produce a solution having a pH of 4 to 7.5.

[0074] The production method is essentially scalable. Using this technology, amounts of HOCl from several quarts to several hundred gallons can be produced. With this HOCl production technology, it is possible to safely produce large amounts of HOCl at low cost.

[0075] In certain aspects of the present disclosure, a pre-measured amount of calcium hypochlorite is dissolved in a predetermined amount of water to produce a calcium hypochlorite solution. In some aspects of the present disclosure, the pre-measured amount of calcium hypochlorite is from about 0.05 g to about 10 g. In some aspects, the pre-measured amount of calcium hypochlorite is from about 0.1 g to about 2.3 g. In some aspects, the pre-measured amount of calcium hypochlorite is from about 0.05 g to about 0.5 g. In some aspects, the pre-measured amount of calcium hypochlorite is from about 0.1 g to about 0.2 g. In some aspects, the pre-measured amount of calcium hypochlorite is from about 1.5 g to about 2 g. In some aspects, the pre-measured amount of calcium hypochlorite is from about 1.7 g to about 1.8 g. In some aspects, the pre-measured amount of calcium hypochlorite is from about 0.25 g to about 0.75 g. In some aspects, the pre-measured amount of calcium hypochlorite is from about 0.4 g to about 0.5 g. In some aspects, the pre-measured amount of calcium hypochlorite is from about 6 g to about 8 g. In some aspects, the pre-measured amount of calcium hypochlorite is from about 6.8 g to about 7.2 g.

[0076] In certain aspects of the present disclosure, a pre-measured amount of calcium hypochlorite is dissolved in water to produce a calcium hypochlorite solution having a concentration of from 0.05 g / L to about 10 g / L. In some aspects, a pre-measured amount of calcium hypochlorite is dissolved in water to produce a calcium hypochlorite solution having a concentration of from about 0.1 g / L to about 2.3 g / L. In some aspects, a pre-measured amount of calcium hypochlorite is dissolved in water to produce a calcium hypochlorite solution having a concentration of from about 0.05 g / L to about 0.5 g / L. In some aspects, a pre-measured amount of calcium hypochlorite is dissolved in water to produce a calcium hypochlorite solution having a concentration of from about 0.1 g / L to about 0.2 g / L. In some aspects, a pre-measured amount of calcium hypochlorite is dissolved in water to produce a calcium hypochlorite solution having a concentration of from about 1.5 g / L to about 2 g / L. In some aspects, a pre-measured amount of calcium hypochlorite is dissolved in water to produce a calcium hypochlorite solution having a concentration of from about 1.7 g / L to about 1.8 g / L.

[0077] Next, the calcium hypochlorite solution is acidified to produce hypochlorous acid. The calcium hypochlorite solution is acidified by adding a pre-measured amount of one or more cation exchange resins to the calcium hypochlorite solution. In some embodiments, the calcium hypochlorite solution is acidified by adding a mixture of a strong acid cation exchange resin (SAC exchange resin) and a weak acid cation exchange resin (WAC exchange resin). In some embodiments, the SAC exchange resin is a hydrogen-form SAC exchange resin. In some embodiments, the WAC exchange resin is a hydrogen-form WAC exchange resin. One of ordinary skill in the art will understand that hydrogen SAC exchange resins are manufactured in a hydrated state and can dehydrate over time. As will be further explained below, the weight of the resin added to the calcium hypochlorite solution is calculated prior to its addition. If the hydrogen SAC resin and / or WAC resin dehydrates excessively over time, one of ordinary skill in the art will understand that an excessive amount of resin is added to the calcium hypochlorite solution, resulting in an increased risk of excessive chlorine gas generation. Therefore, unless otherwise specified, the hydrogen SAC resin used in the disclosed process is in a substantially fully hydrated state.

[0078] In certain aspects of the present disclosure, a pre-measured mixture of WAC exchange resin and SAC exchange resin is added to a calcium hypochlorite solution to obtain a WAC exchange resin concentration of about 0.3 g / L to about 20 g / L and an SAC exchange resin concentration of about 0.3 g / L to 12 g / L. In some aspects, a pre-measured mixture of WAC exchange resin and SAC exchange resin is added to a calcium hypochlorite solution to obtain a WAC exchange resin concentration of about 0.3 g / L to about 2.3 g / L and an SAC exchange resin concentration of about 0.3 to about 1.4 g / L. In some aspects, a pre-measured mixture of WAC exchange resin and SAC exchange resin is added to a calcium hypochlorite solution to obtain a WAC exchange resin concentration of about 6 g / L to about 20 g / L and an SAC exchange resin concentration of about 6 g / L to about 12 g / L. In some aspects, a pre-measured mixture of WAC exchange resin and SAC exchange resin is added to a calcium hypochlorite solution to obtain a WAC exchange resin concentration of about 8 g / L to about 12 g / L and an SAC exchange resin concentration of about 8 g / L to about 10 g / L. In some aspects, a pre-measured mixture of WAC exchange resin and SAC exchange resin is added to a calcium hypochlorite solution to obtain a WAC exchange resin concentration of about 10 g / L and an SAC exchange resin concentration of about 8 g / L. In some aspects, a pre-measured mixture of WAC exchange resin and SAC exchange resin is added to a calcium hypochlorite solution to obtain a WAC exchange resin concentration of about 1.1 g / L and an SAC exchange resin concentration of about 0.9 g / L.

[0079] The step of preparing a hypochlorous acid solution of pre-measured strength and amount can be carried out by using the products and kits of the present disclosure. (a) Open the individual packages of calcium hypochlorite, (b) a mixture of WAC exchange resin and SAC exchange resin, and (c) optionally a surfactant, and pour them into a container. Water is also added to the container. Stir the resulting aqueous mixture of WAC exchange resin, SAC exchange resin, optionally a surfactant, and calcium hypochlorite solution mixture for about 2 minutes to 10 minutes. In some embodiments, the mixture of WAC exchange resin, SAC exchange resin, optionally a surfactant, and calcium hypochlorite solution mixture is stirred for about 3 minutes to 5 minutes. In some embodiments, the mixture of WAC exchange resin, SAC exchange resin, optionally a surfactant, and calcium hypochlorite solution mixture is stirred for about 4 minutes.

[0080] In certain embodiments of the present disclosure, the WAC and SAC exchange resins, and optionally a surfactant are added to a calcium hypochlorite solution and stirred in a non-reactive container. In some embodiments, the mixture is stirred with a non-reactive stirring implement such as a wooden paddle or a glass stirring rod. In certain embodiments of the present disclosure, after acidification, the resin is allowed to settle to the bottom of the container and the hypochlorous acid solution is preferably poured out within about 2 to about 3 minutes of hypochlorous acid formation. In certain embodiments of the present disclosure, the hypochlorous acid solution is passed through a filter to ensure that no resin is contained in the hypochlorous acid solution. In some embodiments, the hypochlorous acid is filtered through a 350 micron filter (45 mesh size) or a 250 micron (60 mesh size) filter. Of course, the filter must be of an appropriate size to prevent the passage of the ion exchange resin.

[0081] In certain embodiments of the present disclosure, the hypochlorous acid can be placed in a non-reactive container and capped with a non-reactive cap and liner, or capped with a non-reactive sprayer and liner, or capped with any other application device that is non-reactive with hypochlorous acid. In some embodiments, the hypochlorous acid can be stored in a non-reactive trigger spray bottle. In one embodiment, the spray bottle is opaque.

[0082] V. Surfactant In certain embodiments of the present disclosure, a pre-measured amount of surfactant is added to a mixture of calcium hypochlorite solution and SAC and WAC exchange resins. In some embodiments, the surfactant is dioctyl sulfosuccinate (e.g., Polywet (trademark) manufactured by Polyventive Plus), alkyldiphenyloxide disulfonate (e.g., Dowfax (trademark) manufactured by Dow), polysorbate (e.g., polysorbate 20 or polysorbate 80), ethoxylated polyoxypropylene block copolymer (e.g., Antarox (registered trademark) manufactured by Solvay), alcohol ethoxylate (e.g., Rhodasurf (registered trademark) manufactured by Solvay), sodium polyacrylate polymer (e.g., Flexisperse (trademark) manufactured by Innovative Chemical Technologies, Inc.), sodium lauriminodipropionate (e.g., Flexisurf (trademark) LDP manufactured by Innovative Chemical Technologies, Inc.), sodium octyliminodipropionate (e.g., Flexisurf (trademark) EHDP manufactured by Innovative Chemical Technologies, Inc.), and combinations thereof.

[0083] In certain embodiments of the present disclosure, a pre-measured amount of surfactant is added to a mixture of calcium hypochlorite solution and SAC and WAC exchange resins in an amount that constitutes from about 0.1 wt% to about 5 wt% of the final hypochlorous acid solution. In some embodiments, the surfactant is added in an amount that constitutes from about 0.5 wt% to about 5 wt% of the final hypochlorous acid solution. In some embodiments, the surfactant is added in an amount that constitutes from about 0.5 wt% to about 2.5 wt% of the final hypochlorous acid solution. In some embodiments, the surfactant is added in an amount that constitutes from about 0.5 wt% to about 1 wt% of the final hypochlorous acid solution.

[0084] VI. Kits and Products of the Present Disclosure In certain aspects of the present disclosure, pre-measured calcium hypochlorite, a pre-measured mixture of WAC exchange resin and SAC exchange resin, and optionally a pre-measured surfactant are individually packaged in a kit. In some aspects, a pre-measured amount of calcium hypochlorite and a mixture of weakly acidic cation exchange resin and strongly acidic cation exchange resin are present in an amount effective to produce hypochlorous acid in an amount sufficient to reduce the measured concentration of microorganisms by 99%, for example, 99.9% when in contact with a surface for about 30 seconds to about 2 minutes. In certain aspects, the amount of calcium hypochlorite, and the mixture of weakly acidic cation exchange resin and strongly acidic cation exchange resin are present in an amount effective to produce hypochlorous acid in an amount sufficient to kill 99.99% of the bacteria on the surface. In the products, kits, and processes of the present disclosure, a pre-measured amount of calcium hypochlorite and a mixture of weakly acidic cation exchange resin and strongly acidic cation exchange resin are present in an amount effective to produce hypochlorous acid in an amount sufficient to kill bacteria, viruses, and fungi to a level of 99.9% of the original number of bacteria, viruses, or fungi on a specific surface area of an inanimate, plant, or animal surface when in contact with the surface for about 30 seconds to about 2 minutes. The components may be included in an amount sufficient to provide a hypochlorous acid solution capable of disinfecting a surface area of about 10 square feet to about 20,000 square feet. In some aspects, pre-measured calcium hypochlorite, pre-measured WAC exchange resin, and pre-measured SAC exchange resin, and optionally a pre-measured surfactant are individually packaged to prevent degradation by moisture, heat, and / or light. The WAC and SAC may be pre-packaged together as a mixture. In some aspects, pre-measured calcium hypochlorite, pre-measured WAC exchange resin, pre-measured SAC exchange resin (or a pre-measured mixture of WAC and SAC exchange resins), and optionally a pre-measured surfactant are individually packaged in a three-layer bag including oriented polypropylene, metallized polyethylene terephthalate, and linear low density polyethylene (e.g., a barrier pouch made by Uline).

[0085] In certain aspects of the present disclosure, the kit includes instructions for preparing hypochlorous acid from kit components. In some aspects, the instructions are printed on a separate sheet within the kit, printed on the packaging of the individual components within the kit, or printed on the kit.

[0086] In certain aspects of the present disclosure, a pre-measured amount of calcium hypochlorite is individually packaged to prevent decomposition by moisture, heat, and / or light. In some aspects of the present disclosure, the pre-measured amount of calcium hypochlorite is from about 0.05 g to about 10 g and is individually packaged. In some aspects, the pre-measured amount of calcium hypochlorite is from about 0.1 g to about 2.3 g and is individually packaged. In some aspects, the pre-measured amount of calcium hypochlorite is from about 0.05 g to about 0.5 g and is individually packaged. In some aspects, the pre-measured amount of calcium hypochlorite is from about 0.1 g to about 0.2 g and is individually packaged. In some aspects, the pre-measured amount of calcium hypochlorite is from about 1.5 g to about 2 g and is individually packaged. In some aspects, the pre-measured amount of calcium hypochlorite is from about 1.7 g to about 1.8 g and is individually packaged. In some aspects, the pre-measured amount of calcium hypochlorite is from about 0.25 g to about 0.75 g and is individually packaged. In some aspects, the pre-measured amount of calcium hypochlorite is from about 0.4 g to about 0.5 g and is individually packaged. In some aspects, the pre-measured amount of calcium hypochlorite is from about 6 g to about 8 g and is individually packaged. In some aspects, the pre-measured amount of calcium hypochlorite is from about 6.8 g to about 7.2 g and is individually packaged.

[0087] In certain aspects of the present disclosure, a pre-measured amount of calcium hypochlorite is individually packaged in an amount sufficient to produce a calcium hypochlorite solution having a concentration of from 0.05 g / L to about 10 g / L when dissolved in water. In some aspects, a pre-measured amount of calcium hypochlorite is individually packaged in an amount sufficient to produce a calcium hypochlorite solution having a concentration of from about 0.1 g / L to about 2.3 g / L when dissolved in water. In some aspects, a pre-measured amount of calcium hypochlorite is individually packaged in an amount sufficient to produce a calcium hypochlorite solution having a concentration of from about 0.05 g / L to about 0.5 g / L when dissolved in water. In some aspects, a pre-measured amount of calcium hypochlorite is individually packaged in an amount sufficient to produce a calcium hypochlorite solution having a concentration of from about 0.1 g / L to about 0.2 g / L when dissolved in water. In some aspects, a pre-measured amount of calcium hypochlorite is individually packaged in an amount sufficient to produce a calcium hypochlorite solution having a concentration of from about 1.5 g / L to about 2 g / L when dissolved in water. In some aspects, a pre-measured amount of calcium hypochlorite is individually packaged in an amount sufficient to produce a calcium hypochlorite solution having a concentration of from about 1.7 g / L to about 1.8 g / L when dissolved in water.

[0088] In certain aspects of the present disclosure, a pre-measured amount of WAC exchange resin and SAC exchange resin is individually packaged (or packaged together as a mixture) to prevent degradation by moisture, heat, and / or light. In some aspects, a pre-measured amount of WAC exchange resin and SAC exchange resin is an amount sufficient to provide a WAC exchange resin concentration of about 0.3 g / L to about 20 g / L and a SAC exchange resin concentration of about 0.3 g / L to about 12 g / L when added to a calcium hypochlorite solution. In some aspects, a pre-measured amount of WAC exchange resin and SAC exchange resin is an amount sufficient to provide a WAC exchange resin concentration of about 0.3 g / L to about 2.3 g / L and a SAC exchange resin concentration of about 0.3 g / L to about 1.4 g / L when added to a calcium hypochlorite solution. In some aspects, a pre-measured amount of WAC exchange resin and SAC exchange resin is an amount sufficient to provide a WAC exchange resin concentration of about 6 g / L to about 20 g / L and a SAC exchange resin concentration of about 6 g / L to about 12 g / L when added to a calcium hypochlorite solution. In some aspects, a pre-measured amount of WAC exchange resin and SAC exchange resin is an amount sufficient to provide a WAC exchange resin concentration of about 8 g / L to about 12 g / L and a SAC exchange resin concentration of about 8 g / L to about 10 g / L when added to a calcium hypochlorite solution. In some aspects, a pre-measured amount of WAC exchange resin and SAC exchange resin is an amount sufficient to provide a WAC exchange resin concentration of about 10 g / L and a SAC exchange resin concentration of about 8 g / L when added to a calcium hypochlorite solution. In some aspects, a pre-measured amount of WAC exchange resin and SAC exchange resin is an amount sufficient to provide a WAC exchange resin concentration of about 1.1 g / L and a SAC exchange resin concentration of about 0.9 g / L when added to a calcium hypochlorite solution.

[0089] In certain aspects of the present disclosure, a pre-measured amount of surfactant is individually packaged and added to a mixture of calcium hypochlorite solution and SAC and WAC exchange resins in an amount that constitutes from about 0.1 wt% to about 5 wt% of the final hypochlorous acid solution. In some aspects, the surfactant is added in an amount that constitutes from about 0.5 wt% to about 5 wt% of the final hypochlorous acid solution. In some aspects, the surfactant is added in an amount that constitutes from about 0.5 wt% to about 2.5 wt% of the final hypochlorous acid solution. In some aspects, the surfactant is added in an amount that constitutes from about 0.5 wt% to about 1 wt% of the final hypochlorous acid solution.

[0090] VII. Hypochlorous Acid In certain aspects of the present disclosure, a pre-measured amount of calcium hypochlorite and a mixture of weakly acidic cation exchange resin and strongly acidic cation exchange resin are present in an amount effective to produce hypochlorous acid in an amount sufficient to reduce the measured concentration of microorganisms by 99%, for example, 99.9% when in contact with a surface for about 30 seconds to about 2 minutes. In certain aspects, the amount of calcium hypochlorite and the mixture of weakly acidic cation exchange resin and strongly acidic cation exchange resin are present in an amount effective to produce hypochlorous acid in an amount sufficient to kill 99.99% of the bacteria on the surface. In the products, kits, and processes of the present disclosure, a pre-measured amount of calcium hypochlorite and a mixture of weakly acidic cation exchange resin and strongly acidic cation exchange resin are present in an amount effective to produce hypochlorous acid in an amount sufficient to kill bacteria, viruses, and fungi to a level of 99.9% of the original number of bacteria, viruses, or fungi on a specific surface area of inanimate, plant, or animal surfaces when in contact with the surface for about 30 seconds to about 2 minutes. In some aspects, the hypochlorous acid produced in accordance with the present disclosure has a free available chlorine (FAC) concentration of about 50 to about 7000 ppm. In some aspects, the hypochlorous acid produced in accordance with the present disclosure has an FAC concentration of about 50 to 200 ppm. In some aspects, the hypochlorous acid produced in accordance with the present disclosure has an FAC concentration of about 50 to about 100 ppm. In some aspects, the hypochlorous acid produced in accordance with the present disclosure has an FAC concentration of about 50 to about 1500 ppm, or about 500 ppm to about 1200 ppm. In some aspects, the hypochlorous acid produced in accordance with the present disclosure has an FAC concentration of about 200 to about 1100 ppm, or about 700 to about 1100 ppm. In some aspects, the hypochlorous acid produced in accordance with the present disclosure has an FAC concentration of about 700 to about 1000 ppm.

[0091] In certain aspects of the present disclosure, the hypochlorous acid produced in accordance with the present disclosure has a pH of about 3 to about 8. In some aspects, the hypochlorous acid has a pH of about 4 to 7.

[0092] In certain aspects of the present disclosure, the hypochlorous acid generated in accordance with the present disclosure can be further diluted to a preferred FAC concentration. One of ordinary skill in the art will understand how to determine the required amount of water to be added to the hypochlorous acid solution to dilute it to a given FAC concentration.

[0093] In certain aspects of the present disclosure, the FAC concentration of the hypochlorous acid solution prepared in accordance with the present disclosure does not decrease by more than 50%, more than 40%, more than 30%, more than 20%, or more than 10% when stored at room temperature for 48 hours. In some aspects, the FAC concentration of the hypochlorous acid does not decrease by more than 30%, more than 20%, more than 10%, or more than 5% when stored at room temperature for 24 hours.

[0094] VIII. Use of Hypochlorous Acid Hypochlorous acid solutions have a wide range of uses, including sterilization, disinfection, and contamination removal. Examples of such uses include hospitals, nursing homes, long-term care facilities, emergency medical facilities, ambulances, medical laboratories, clinics, hospitals, dental facilities, and other healthcare settings; daycare facilities and educational institutions, customer service venues (such as restaurants, hotels, and cruise ships); retail food facilities (grocery stores, convenience stores); agricultural environments including livestock barns, veterinary facilities, and plant and seed treatment facilities; gyms and training facilities; architectural offices; private homes, and disinfection and sterilization in bathrooms. Further, hypochlorous acid solutions can be applied directly or sprayed onto plants (leaves, stems, flowers, and fruits), for example, in the floral industry, to extend the shelf life of flowers. Hypochlorous acid solutions can also be applied directly or sprayed onto animals, for example, for skin disinfection, wound care, and burn treatment.

[0095] Hypochlorous acid is known to effectively kill a wide variety of microorganisms at various contact times (i.e., the time the hypochlorous acid is in contact with the microorganisms). When testing the hypochlorous acid solution generated in accordance with the present disclosure, it was found to be effective against the following microorganisms.

Table 1

Example

[0096] Example 1 Steps for generating hypochlorous acid. Hypochlorous acid was generated according to the following steps: 1. Pour 1 L of water into a non-reactive mixing container. 2. Add 1.7 g of calcium hypochlorite to the mixing container and stir with a wooden paddle for about 30 seconds. 3. Add a pre-measured mixture of 10 g of WAC exchange resin and 8 g of SAC exchange resin to the mixing container. Immediately start stirring with a wooden paddle and stir for 4 minutes. 4. After allowing the resin to settle to the bottom of the mixing container, pour the hypochlorous acid solution into a second non-reactive container for use. The hypochlorous acid solution may be filtered through an appropriate filter to remove the resin.

[0097] The hypochlorous acid was stored in a non-reactive container at room temperature, and the FAC concentration was monitored over time. In Figures 1 and Table 2 below, when stored at room temperature for 48 hours, the FAC concentration decreased by approximately 12% from 900 ppm to 791 ppm.

Table 2

[0098] Example 2 Preparation of hypochlorous acid solution from a kit The hypochlorous acid solution is prepared using a method that includes the following steps similar to Example 1, except that the solid components (1. calcium hypochlorite, 2. ion exchange resin, and 3. surfactant) are individually pre-packaged in a three-layer barrier pouch made by Uline. Step 1 involves mixing the pre-packaged components and adding all the components into a container together with a predetermined amount of water. The main purpose of this step is to prepare the hypochlorous acid solution by reacting the WAC exchange resin and SAC exchange resin with calcium hypochlorite, which is achieved by stirring well so that all the components dissolve properly. The surfactant can be optionally added during this step. The surfactant helps to wet the hydrophobic surfaces. Step 2 involves decanting or removing by other means the WAC exchange resin and SAC exchange resin that do not dissolve during the formation of the hypochlorous acid solution.

[0099] Example 3 Testing of Hypochlorous Acid Solution from the Kit A film of bacterial cells or a film of dried virus on the surface of a glass carrier was exposed to the test substance for a specified exposure time or for 30 seconds or for 1 minute. The test substance was the solution of Example 1 sprayed 4 times from a distance of 6 - 8 inches from the carrier surface via a trigger spray bottle. After exposure, the carrier was transferred to a container containing a neutralizing subculture medium. The subculture was incubated and the remaining ones were measured. Appropriate culture purity, viability, organic soil load sterility, sterility of the neutralizing subculture medium, carrier sterility, carrier population, and neutralization confirmation controls were performed. Also, the results of these tests are included in Table 1 above.

[0100] Other Aspects All publications, patents, and patent applications mentioned in this specification are hereby incorporated by reference in their entirety to the same extent as if each individual publication, patent, or patent application was specifically and individually indicated to be incorporated by reference in its entirety. If a term in this application is found to have a different definition in a document incorporated by reference into this specification, the definition provided in this specification shall function as the definition of that term.

[0101] Although the invention has been described in connection with its specific embodiments, it will be understood that further modifications are possible. Also, this application is generally intended to cover any variations, uses, or adaptations of the invention, including departures from the present disclosure that come within the known or customary practice within the technical field to which the invention pertains and fall within the scope of the claims, provided they are made in accordance with the principles of the invention and are within the scope of the essential features shown above.

Claims

1. A kit comprising a) a pre-measured amount of calcium hypochlorite, b) a pre-measured amount of weakly acidic cation exchange resin and a pre-measured amount of strongly acidic cation exchange resin, and c) instructions for preparing hypochlorous acid, wherein component a) is packaged separately from component b), and the weakly acidic cation exchange resin and the strongly acidic cation exchange resin can be packaged together as a mixture or packaged separately from each other.

2. The kit according to claim 1, wherein the solution obtained according to the instructions for preparing a hypochlorous acid solution has a free available chlorine concentration of about 7,000 ppm to about 50 ppm.

3. The kit according to claim 1, wherein the solution obtained according to the instructions for preparing a hypochlorous acid solution has a free available chlorine concentration of about 1000 to 700 ppm.

4. The kit according to claim 1, wherein the solution obtained according to the instructions for preparing a hypochlorous acid solution has a free available chlorine concentration of about 1000 to 200 ppm.

5. The kit according to claim 1, wherein the solution obtained according to the instructions for preparing a hypochlorous acid solution has a free available chlorine concentration of about 7,000 to 4,000 ppm.

6. The kit according to claim 5, wherein the solution obtained according to the instructions for preparing a hypochlorous acid solution has a free available chlorine concentration of about 200 to 50 ppm.

7. The kit according to claim 1, wherein the hypochlorous acid solution has a pH of about 4 to about 7.

8. The kit according to claim 3, comprising the aforementioned pre-measured amount of calcium hypochlorite in an amount that provides approximately 1.7 g / L to approximately 1.8 g / L in a predetermined amount of water.

9. The kit according to claim 8, comprising a mixture of the previously measured weakly acidic cation exchange resin and strongly acidic cation exchange resin in an amount that provides about 6 g / L to about 20 g / L of weakly acidic cation exchange resin and about 6 g / L to about 12 g / L of strongly acidic cation exchange resin per predetermined amount of water.

10. The kit according to claim 9, comprising a mixture of the previously measured weakly acidic cation exchange resin and strongly acidic cation exchange resin in an amount equal to about 10 g of weakly acidic cation exchange resin / 1 L of water, based on a predetermined amount of water, and an amount equal to about 8 g of strongly acidic cation exchange resin / L, based on a predetermined amount of water.

11. The kit according to claim 6, wherein the amount of calcium hypochlorite measured in advance is approximately 0.1 g / L to approximately 0.2 g / L in a predetermined amount of water.

12. The kit according to claim 11, comprising a mixture of the previously measured weakly acidic cation exchange resin and strongly acidic cation exchange resin in an amount that provides 0.3 g / L to about 2.3 g / L of weakly acidic cation exchange resin and about 0.3 g / L to about 1.4 g / L of strongly acidic cation exchange resin per predetermined amount of water.

13. The kit according to claim 12, comprising a mixture of the previously measured weakly acidic cation exchange resin and strongly acidic cation exchange resin in an amount equal to about 1.1 g of weakly acidic cation exchange resin / 1 L of water, and an amount equal to about 0.9 g of strongly acidic cation exchange resin / L, based on a predetermined amount of water.

14. The kit according to claim 1, wherein the weakly acidic cation exchange resin is a hydrogen form of a weakly acidic cation exchange resin and contains a carboxylic acid functional group, and the strongly acidic cation exchange resin is a hydrogen form of a strongly acidic cation exchange resin and contains a sulfonic acid functional group.

15. d) The kit according to claim 1, further comprising a pre-measured amount of surfactant.

16. The kit according to claim 1, wherein a mixture of a weakly acidic cation exchange resin and a strong cation exchange resin of component a) and component b), and optionally a pre-measured amount of surfactant of component d), are each packaged in separate airtight and watertight containers.

17. The kit according to claim 1, wherein the weakly acidic cation exchange resin of component a) and component b), the strong cation exchange resin component of component b), and optionally component d) a pre-measured amount of surfactant are each packaged in separate airtight and watertight containers.

18. The kit according to claim 16, wherein each of the airtight and watertight containers is a three-layer bag comprising oriented polypropylene, metallized polyethylene terephthalate, and linear low-density polyethylene.

19. A step for producing hypochlorous acid, comprising dissolving a pre-measured amount of calcium hypochlorite in water, adding a pre-measured amount of a mixture of a weakly acidic cation exchange resin and a strongly acidic cation exchange resin to the water, and stirring, wherein the pre-measured amounts of calcium hypochlorite, the mixture, and water are effective in producing a hypochlorous acid solution having a pH of about 4 to about 7 when stirring is stopped.

20. The kit according to claim 1, wherein the previously measured amount of calcium hypochlorite and the mixture of a weakly acidic cation exchange resin and a strongly acidic cation exchange resin are present in an amount sufficient to generate hypochlorous acid to reduce the measured microbial concentration by 99% or more when in contact with the surface for approximately 30 seconds to approximately 2 minutes.

21. The step according to claim 19, wherein the previously measured amount of calcium hypochlorite and the mixture of a weakly acidic cation exchange resin and a strongly acidic cation exchange resin are present in an amount sufficient to generate hypochlorous acid to reduce the measured microbial concentration by 99% or more when in contact with the surface for about 30 seconds to about 2 minutes.

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