Method of elimination of cryphonectria parasitica fungi with inula viscosa l. plant water extract

Abstract

This invention relates to a method of eliminating the Cryphonectria parasitica fungus with Inula viscosa L. plant water extract (100), which prevents diseases caused by a parasitic fungus called Cryphonectria parasitica, which is encountered in the field of agriculture and food.

Description

[0001] METHOD OF ELIMINATION OF CRYPHONECTRIA PARASITICA FUNGI WITH INULA VISCOSA L. PLANT WATER EXTRACT

[0002] Technical Field

[0003] This invention relates to a method of eliminating diseases caused by a parasitic fungus called Cryphonectria parasitica, used in agriculture and food, with the water extract of the Inula viscosa L. plant.

[0004] Prior Art

[0005] The disease caused by a parasitic fungus called Cryphonectria parasitica, can be carried by rain, wind, birds and insects and spread rapidly in agricultural and farming areas where food products are grown. The tools used during pruning and the sticks used during harvesting, which are used to prevent or prevent the spread of said diseases, also cause contamination. It even accelerates contamination by causing injury in the tree trunk, and for these reasons, the disease cannot be eliminated with a method that can be applied in large areas and give definitive results. Chemical pesticides used in the control leave residues on the plants and therefore the fruits to which they are applied and reduce the quality of the products even if the pathogen is eliminated. For example, the application of blue vitriol results in the Cu (Copper) heavy metal contained in the composition of this chemical contaminating trees and fruits and leaving residues, and also causes a Copper level above natural values to reach the forest ecosystem. Cryphonectria parasitica, which is seen in oak, maple and hornbeam trees as well as chestnut trees, forms a threat to the forests of Turkiye and the Earth in terms of forest existence, wood and non-wood products.

[0006] The disease caused by a parasitic fungus called Cryphonectria parasitica, known abroad as Chestnut Blight, Murrill Barr, and in our country as Chestnut Cancer, causes great damage to oak, maple and hornbeam trees, especially chestnuts. The fungus, which settles on the plant bark through cracks or injured areas on the trunk and branches, destroys the cambium tissue, spreads to the transmission tissues, causes the branches, roots and eventually the entire plant to die.

[0007] The occurrence of the disease in chestnut trees, which are more than 2 million in spread state over an area of 262 thousand hectares in the Black Sea and Aegean Regions of our country and are an important source of income, causes serious economic losses for farmers. Suggestions for solution by the Ministry of Agriculture and Forestry of Turkiye are listed as cutting the diseased branches, scraping the diseased area and applying a mixture of Blue vitriol + Juniper Tar and using hypo virulent races. The fighting techniques applied abroad are similar to those in our country, such as pruning or spraying trees.

[0008] In the method of eliminating Cryphonectria parasitica fungus with Inula viscosa L. plant water extract, which is included in the invention subject to the application, Inola viscosa L. plant is produced by extracting only with water. The method in the invention, which is subject to the application is both extremely natural, has a low environmental footprint and is economical since it does not use chemical solvents, and there is no use of emissions, waste or high-priced consumables that occur during the production of chemical pesticides. The obtained Inola viscosa L. plant water extract is applied to the areas where the Cryphonectria parasitica fungus is found on the tree to control the growth of the fungus. As a result, the reproduction of the Cryphonectria parasitica fungus is prevented by Inola viscosa L. plant water extract.

[0009] In the present art, there is no description related to the technical features included in the invention subject to the application and the technical effects provided by the invention subject to the application. In present embodiments, there is no method that uses natural plant extracts to prevent the reproduction or spread of the Cryphonectria parasitica fungus.

[0010] Objects of the Invention

[0011] The object of this invention is to perform the method of eliminating the Cryphonectria parasitica fungus with the Inula viscosa L. plant water extract produced by extracting only with water.

[0012] Another object of this invention is to be both extremely natural, have a low environmental footprint and economical since it does not use chemical solvents, and to perform a method of eliminating Cryphonectria parasitica fungus with Inula viscosa L. plant water extract, which prevents emissions, wastes and the use of high-priced consumables occurred during the production of chemical pesticides.

[0013] Another object of this invention is to perform the method of eliminating Cryphonectria parasitica fungus with Inula viscosa L. plant water extract, which enables the Inola viscosa L. plant water extract to be produced at very low costs as it is obtained at room temperature and prevents energy consumption for purposes such as heating and boiling during production.

[0014] Another object of this invention is to perform a method of eliminating Cryphonectria parasitica fungus with Inula viscosa L. plant water extract, which does not require any special agricultural activity to obtain the extract and is applied only by collecting the plant from nature.

[0015] Another object of the invention is to perform a method of eliminating the Cryphonectria parasitica fungus with Inula viscosa L. plant water extract preventing the spread and reproduction of the Cryphonectria parasitica fungus, which creates very negative economic effects for chestnut producers by causing the loss of productive branches of trees or their complete death.

[0016] Another object of the invention is to perform a method of eliminating Cryphonectria parasitica fungus with Inula viscosa L. plant water extract, which Inola viscosa L. extract enables the elimination of Cryphonectria parasitica fungus even at low doses.

[0017] Another object of the invention is to perform a method of eliminating Cryphonectria parasitica fungus with Inula viscosa L. plant water extract, which is easier and cheaper than previous applications, as it is possible to apply Inola viscosa L. extract at low doses and easily by spraying.

[0018] Another object of the invention is to perform a method of eliminating Cryphonectria parasitica fungus with Inula viscosa L. plant water extract, which can prevent the spread of the disease by applying Inola viscosa L extract not only to trees and branches infected with the Cryphonectria parasitica fungus, but also to other trees and plants around these trees.

[0019] There is no need to use previous techniques that damage trees, such as cutting down branches or the entire tree infected with the Cryphonectria parasitica fungus or scraping the tree surface with this invention.

[0020] There is no need to spray trees infected with the Cryphonectria parasitica fungus by using harmful chemicals with this invention.

[0021] There is no need to quarantine the area where the Cryphonectria parasitica fungus infects the trees with this invention.

[0022] Inola viscosa L. plant extract can be produced at normal ambient temperature by using only water and without the need for heating with this invention.

[0023] Cryphonectria parasitica fungus can be eliminated from the environment with a single dose of Inola viscosa L. extract having very low concentration with this invention.

[0024] Inola viscosa L. extract can be applied not only to diseased trees, but also to other plants in the environment and to tools used for pruning and harvesting, thus preventing the spread of the disease through contamination with this invention.

[0025] The Inola viscosa L. extract included in this invention is completely natural, cheap and easily applicable.

[0026] Said Inola viscosa L. plant included in this invention grows easily and spontaneously in the Aegean and Mediterranean climate regions, therefore it does not need irrigation and is abundant. Brief Description of the Invention

[0027] In the method of eliminating the Cryphonectria parasitica fungus with an Inula viscosa L. plant water extract defined in the first claim and other claims related to this claim, which is carried out to achieve the object of this invention, first, Inola viscosa L. root stems and leaves were collected, washed, dried and ground into powder, shaken with pure water at room temperature for 24 hours, centrifuged, filtered and concentrated, then frozen at -80°C and evaporated in a lyophilizer. The final dry agent amount of the leaf water extract, which gave the most successful result, was 165 g / L. Cryphonectria parasitica was identified by isolating on PDA (Potato Dextrose Agar) Agar medium. Minimum inhibition concentration (MIC) CP standardization is suitable for the 0.5 McFarland standard and the amount of live cells in the test tube is between 1x107-1x108cfu / ml. In the MIC method, 100 pL of Mueller Hinton Broth was loaded into the microplate wells, after 200 pL from the extract was added to the wells in the first column, 100 pL was taken from the twelfth well (including the 12th well) and diluted by transferring it to the next one. 100 pL of content taken from the 12th well was put out. Each dose was run three times in parallel, and the microplates were incubated at 27°C for 12 hours. Negative and positive controls were also applied. No growth was observed in the first 3 wells of the leaf extract and the MIC value was 0.020725 mg / pL.

[0028] Detailed Description of the Invention

[0029] The method of eliminating Cryphonectria parasitica fungus with Inula viscosa L. plant water extract, which was carried out to achieve the object of this invention, is shown in the attached drawings and these drawings are as follows;

[0030] Figure 1. Schematic view of the method of eliminating Cryphonectria parasitica fungus with Inula viscosa L. plant water extract.

[0031] The parts in the figures are numbered one by one, and the equivalents of these numbers are given below.

[0032] 100. Method of eliminating Cryphonectria parasitica fungus with Inula viscosa L. plant water extract

[0033] 101. Collecting the roots, stems and leaves of the Inola viscosa L. plant, washing, drying and grinding the collected roots, stems and leaves into powder.

[0034] 102. Passing the Inola viscosa L. plant extract into water by shaking the Inola viscosa L. plant powder obtained by grinding with pure water at room temperature for 24 hours.

[0035] 103. After shaking, carrying out centrifugation to separate the water containing Inola viscosa L. plant extract and applying filtration and concentration processes. 104. Obtaining Inola viscosa L plant water extract

[0036] 105. Obtaining a water extract of Inola viscosa L. plant that has the effect of eliminating the Cryphonectria parasitica fungus.

[0037] This invention is a method of eliminating the Cryphonectria parasitica fungus with Inula viscosa L. plant water extract (100), which prevents diseases caused by a parasitic fungus called Cryphonectria parasitica, which is used in the field of agriculture and food, wherein;

[0038] - Collecting the roots, stems and leaves of the Inola viscosa L. plant, washing, drying and grinding the collected roots, stems and leaves into powder (101),

[0039] - Shaking the Inola viscosa L. plant powder obtained by grinding with pure water at room temperature for 24 hours (102),

[0040] - After shaking, carrying out centrifugation to separate the water containing Inola viscosa L. plant extract and applying filtration and concentration processes (103),

[0041] - Obtaining Inola viscosa L plant water extract (104),

[0042] - Comprising the process steps of obtaining the water extract of the Inola viscosa L. plant that has the effect of eliminating the Cryphonectria parasitica fungus (105),

[0043] - Transferring the obtained extract onto the Cryphonectria parasitica fungus on trees and plants and preventing and eliminating the reproduction of the Cryphonectria parasitica fungus with the effect of the transferred Inola viscosa L. plant water extract.

[0044] The method of eliminating the Cryphonectria parasitica fungus with the Inula viscosa L. plant water extract subject to the application (100) enables obtaining the Inula viscosa L. plant water extract produced by extracting only with water. In the method of eliminating Cryphonectria parasitica fungus with Inula viscosa L. plant water extract (100) subject of the application, it is both extremely natural, has a low environmental footprint and is economical since it does not use chemical solvents, and there is no use of emissions, waste or high- priced consumables that occur during the production of chemical pesticides. In the method of eliminating Cryphonectria parasitica fungus with Inula viscosa L. plant water extract (100) subject of the application, it is provided that the Inola viscosa L. plant water extract is produced at very low costs as it is obtained at room temperature and energy consumption is prevented for purposes such as heating and boiling during production. In the method of eliminating Cryphonectria parasitica fungus with Inula viscosa L. plant water extract (100) subject of the application, there is no need for any special agricultural activity to obtain the extract and it is applied only by collecting the plant from nature. In the method of eliminating Cryphonectria parasitica fungus with Inula viscosa L. plant water extract (100) subject of the application, the spread and reproduction of the Cryphonectria parasitica fungus, which creates very negative economic effects for chestnut producers by causing the loss of productive branches of trees or their complete death is naturally prevented. In the method of eliminating Cryphonectria parasitica fungus with Inula viscosa L. plant water extract (100) subject of the application, Inola viscosa L. extract enables the elimination of Cryphonectria parasitica fungus even at low doses. In the method of eliminating Cryphonectria parasitica fungus with Inula viscosa L. plant water extract (100) subject of the application, it is easier and cheaper than previous applications, as it is possible to apply Inola viscosa L. extract at low doses and easily by spraying. In the method of eliminating Cryphonectria parasitica fungus with Inula viscosa L. plant water extract (100) subject of the application, Inula viscosa L. plant water extract can be applied not only to trees and branches infected with the Cryphonectria parasitica fungus, but also to other trees and plants around these trees.

[0045] In the method of eliminating Cryphonectria parasitica fungus with Inula viscosa L. plant water extract (100) included in an embodiment of the invention, first the root, stem and leaves of the Inola viscosa L. plant are collected, the collected roots, stem and leaves are washed, dried and ground into powder (101). 165 grams of dry Inola viscosa L. plant powder obtained by grinding is shaken with 1 Liter of pure water at room temperature for 24 hours (102). After shaking, the water is separated by centrifugation and / or physical separation processes such as filtration and concentration are applied (103). In the process step (103), after the concentration process, it is ensured that the concentrated liquid comprising Inola viscosa L. extract contains at least 0.020725 mg / pL of dry agent of Inola viscosa L. extract (0.020725 mg of dry Inola viscosa L. extract / mL of water). Inola viscosa L. plant water extract is obtained (105). A water extract of the Inola viscosa L. plant that has the effect of eliminating the Cryphonectria parasitica fungus is obtained (106). By transferring the Inola viscosa L extract obtained onto the Cryphonectria parasitica fungus on trees, branches and plants, the reproduction of the Cryphonectria parasitica fungus is prevented and eliminated.

Claims

CLAIMS1. This invention is a method of eliminating the Cryphonectria parasitica fungus with Inula viscosa L. plant water extract (100), which prevents diseases caused by a parasitic fungus called Cryphonectria parasitica, which is used in the field of agriculture and food, wherein;- Collecting the roots, stems and leaves of the Inola viscosa L. plant, washing, drying and grinding the collected roots, stems and leaves into powder (101),- Shaking the Inola viscosa L. plant powder obtained by grinding with pure water at room temperature for 24 hours (102),- After shaking, carrying out centrifugation to separate the water containing Inola viscosa L. plant extract and applying filtration and concentration processes (103),- Obtaining Inola viscosa L plant water extract (104),- Comprising the process steps of obtaining the water extract of the Inola viscosa L. plant that has the effect of eliminating the Cryphonectria parasitica fungus (105),- Applying the obtained extract onto the Cryphonectria parasitica fungus on trees and plants and preventing and eliminating the reproduction of the Cryphonectria parasitica fungus by applying Inola viscosa L. plant water extract.

Images