Method for extraction and purification of tetrodotoxin
The method uses organic solvents with limited water content for TTX extraction and silica gel chromatography to improve recovery and stability, addressing inefficiencies in existing TTX extraction and purification processes.
JP2025524501APending Publication Date: 2025-07-30WEX PHARMACEUTICALS INC
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Patent Information
- Application Number
- JP2024576699
- Authority / Receiving Office
- JP · JP
- Patent Type
- Applications
- Current Assignee / Owner
- Priority Date
- 2022-07-06
- Filing Date
- 2023-04-26
- Publication Date
- 2025-07-30
AI Technical Summary
Technical Problem
Existing methods for extracting and purifying tetrodotoxin (TTX) from biological materials face inefficiencies, such as low yields, solvent requirements, and instability of TTX due to high water content, leading to decomposition and difficulty in separation and purification.
Method used
A method involving the use of organic solvents with limited water content for extraction, followed by solvent washing and silica gel chromatography, and optionally preparative HPLC, to enhance TTX recovery and stability.
Benefits of technology
This method achieves higher TTX recovery rates and purity, reducing solvent usage and avoiding resin-based chromatography drawbacks, while maintaining TTX stability and efficiency.
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Smart Images
Figure 2025524501000001_ABST
Abstract
A method for extracting TTX from a biological material containing TTX, the method including a step of extracting with a first solvent, a step of concentrating, a step of washing with a second solvent, a step of concentrating again, and a step of purifying by chromatography.
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