Heterocyclic compounds, compositions thereof, and methods of treatment therewith

Abstract

As used herein, the following structure: TIFF2025528126000440.tif80165, wherein the substituents are as defined herein, compositions comprising an effective amount of the compound, and methods for modulating the activity of KRAS G12D and / or G12V are provided.

Description

[Technical Field]

[0001] Provided herein are heterocyclic compounds useful for treating cancer, pharmaceutical compositions containing the compounds, and methods of using the compounds to treat cancer or conditions treatable or preventable by inhibition of KRAS activity, comprising administering an effective amount of the compound to a subject in need thereof. [Background technology]

[0002] Ras is a family of proteins associated with the cell membrane through their C-terminal membrane-targeting domains and is well-known as a molecular switch in intracellular signaling networks (Cox AD, Der CJ. Ras history: The saga continues. Small GTPases. 2010;1(1):2-27). Ras proteins bind either GTP or GDP to switch between "on" and "off" states. When a Ras protein binds to GDP, it is in the off (or inactive) state. When Ras is switched on by certain growth-promoting stimuli, such as growth factors, the Ras protein is induced to exchange its bound GDP for GTP, changing to the on (or active) state (Malumbres M, Barbacid M. RAS oncogenes: the first 30 years. Nat Rev Cancer. 2003;3(6):459-465). Upon switching to an active state, Ras proteins can interact with various downstream proteins and activate associated signaling pathways (Berndt N, Hamilton AD, Sebti SM. Targeting protein prenylation for cancer therapy. Nat Rev Cancer. 2011;11(11):775-791). The Ras superfamily comprises various subfamilies, including Ras, Ral, Rap, Rheb, Rad, Rit, and Miro (Wennerberg K, Rossman KL, Der CJ. The Ras superfamily at a glance. J Cell Sci. 2005;118(Pt 5):843-846). HRas, NRas, and KRas are the best-studied proteins in the Ras family because these proteins are the most common oncogenes in human cancers (O'Bryan JP. Pharmacological targeting of RAS: Recent success with direct inhibitors. Pharmacol Res. 2019;139:503-511).

[0003] KRas is one of the most frequently mutated genes in human cancer. Based on data from the Catalogue of Somatic Mutations (COSMIC) database, KRas mutations can be found in approximately 20% of human cancers, including pancreatic, colorectal, lung, and skin cancers (O'Bryan JP. Pharmacological targeting of RAS: Recent success with direct inhibitors. Pharmacol Res. 2019;139:503-511). The most common KRas mutations are found at G12 and G13 positions, inhibiting the GTP hydrolysis activity of KRas stimulated by GTPase-activating proteins (GAPs) (Wang W, Fang G, Rudolph J. Ras inhibition via direct Ras binding—is there a path forward?. Bioorg Med Chem Lett. 2012;22(18):5766-5776). This leads to overactivation of the KRas protein, ultimately causing uncontrolled cell growth and cancer.

[0004] Among various cancers, pancreatic cancer is considered the most KRas-dependent. KRas mutations are found in 94.1% of pancreatic ductal adenocarcinoma (PDAC). KRas G12D (41%) and G12V (34%) mutations are the two most prevalent mutations in all KRas-mutated PDAC (Waters AM, Der CJ. KRAS: The Critical Driver and Therapeutic Target for Pancreatic Cancer. Cold Spring Harb Perspect Med. 2018;8(9):a031435). In vivo data from mouse models demonstrate that the progression and maintenance of pancreatic cancer are highly dependent on constitutive activation of KRas downstream signaling (Siveke JT, Schmid RM. Chromosomal instability in mouse metastatic pancreatic cancer—it's KRAS and Tp53 after all. Cancer Cell. 2005;7(5):405-407). This indicates that mutant KRas proteins are very attractive drug targets for pancreatic cancer and other cancers that also harbor KRas mutations. Because wild-type KRas proteins also play important roles in normal tissue function and wild-type KRas function has been demonstrated to be essential for adult hematopoiesis (Malumbres M, Barbacid M. RAS oncogenes: the first 30 years. Nat Rev Cancer. 2003;3(6):459-465), it would be highly valuable for drug candidates to selectively inhibit mutant KRas proteins in cancer cells while sparing their wild-type counterparts in normal cells.

[0005] Therefore, the KRas G12D and G12V mutations are very attractive targets for this and other cancers, and small molecule therapeutics that can selectively bind to and inhibit the function of Kras G12D or G12V would be very useful.

[0006] Citation or identification of any reference in this section of this application shall not be construed as an admission that the reference is prior art to the present application. Summary of the Invention

[0007] As used herein, the following formula (I): [ka] and pharmaceutically acceptable salts, tautomers, stereoisomers, enantiomers, atropisomers, isotopically substituted variants, and prodrugs thereof, wherein the substituents are as defined herein.

[0008] In one embodiment, the compound is selected from Tables 1-3.

[0009] In one embodiment, provided herein is a method for inhibiting the activity of a KRAS mutant protein or KRAS amplification in a cell, comprising contacting the cell with an effective amount of a compound provided herein, or a pharmaceutically acceptable salt, tautomer, isotope, stereoisomer, enantiomer, atropisomer, or prodrug thereof, wherein optionally the KRAS mutant protein is a KRAS G12D and / or G12V mutant protein.

[0010] In one embodiment, provided herein is a method for the treatment or prevention of cancer, comprising administering to a subject in need thereof an effective amount of a compound provided herein, or a pharmaceutically acceptable salt, tautomer, isotopic substitute, stereoisomer, enantiomer, atropisomer, or prodrug thereof, wherein optionally the cancer is mediated by a KRAS mutation, preferably a KRAS G12D and / or G12V mutation. DETAILED DESCRIPTION OF THE INVENTION

[0011] definition As used herein, "KRAS gene" refers to a gene selected from the group consisting of DIRAS1; DIRAS2; DIRAS3; ERAS; GEM; HRAS; KRAS; MRAS; NKIRAS1; NKIRAS2; NRAS; RALA; RALB; RAP1A; RAP1B; RAP2A; RAP2B; RAP2C; RASD1; RASD2; RASL10A; RASL10B; RASL11A; RASL11B; RASL12; REM1; REM2; RERG; RERGL; RRAD; RRAS; RRAS2, and mutants thereof.

[0012] As used herein, "KRAS protein" refers to a protein expressed by the KRAS gene or an isoform thereof (Scolnick EM, Papageoege AG, Shih TY (1979), "Guanine nucleotide-binding activity for src protein of rat-derived murine sarcoma viruses," Proc Natl Acad Sci USA. 76(5):5355-5559; Kranenburg O (November 2005) "The KRAS oncogene: past, present, and future," Biochimica et Biophysica Acta (BBA) - Reviews on Cancer, 1756 (2):81-2).

[0013] As used herein, "G12D mutation" refers to a mutation of the 12th amino acid residue, located in the G domain of the KRAS protein, from glycine to aspartic acid.

[0014] As used herein, "KRAS G12D" or "G12D" refers to a KRAS protein having a G12D mutation.

[0015] As used herein, "G12V mutation" refers to a mutation of the 12th amino acid residue, located in the G domain of the KRAS protein, from glycine to valine.

[0016] As used herein, "KRAS G12V" or "G12V" refers to a KRAS protein having a G12V mutation.

[0017] As used herein, "KRAS amplification" or "KRAS gene amplification" refers to a genetic change that increases the copy number of the KRAS gene in some cancer cells. This can lead to higher expression and activity of the KRAS protein, which is involved in cell proliferation and survival. KRAS amplification is found in some types of cancer, such as lung cancer, breast cancer, esophageal cancer, ovarian cancer, and testicular cancer.

[0018] As used herein and in the specification and appended claims, the indefinite articles "a" and "an" and the definite article "the" include plural references and singular referents unless the context clearly dictates otherwise.

[0019] As used herein, and unless otherwise specified, the terms "about" and "approximately," when used in reference to a dose, amount, or weight percent of a component of a composition or dosage form, mean a dose, amount, or weight percent that one of ordinary skill in the art would recognize as providing an equivalent pharmacological effect to that obtained by the specific dose, amount, or weight percent. In certain embodiments, when used in this context, the terms "about" and "approximately" contemplate a dose, amount, or weight percent that is within 30%, within 20%, within 15%, within 10%, or within 5% of the specific dose, amount, or weight percent.

[0020] As used herein, and unless otherwise specified, the terms "about" and "approximately," when used in reference to a numerical value or range of values ​​provided to characterize a particular solid form, e.g., a particular temperature or temperature range, e.g., a particular temperature or temperature range representing a melting temperature, dehydration temperature, desolvation temperature, or glass transition temperature; mass change, e.g., mass change as a function of temperature or humidity; solvent or water content, e.g., in terms of mass or percentage; or peak position, e.g., peak position in analysis by IR spectroscopy or Raman spectroscopy or XRPD, indicates that the value or range of values ​​may deviate to an extent that would be considered reasonable by one of ordinary skill in the art and still describe the solid form. Techniques for characterizing crystalline forms and amorphous solids include, but are not limited to, thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), X-ray powder diffractometry (XRPD), single crystal X-ray diffraction, vibrational spectroscopy such as infrared (IR) spectroscopy and Raman spectroscopy, solid and solution nuclear magnetic resonance (NMR) spectroscopy, optical microscopy, hot-stage optical microscopy, scanning electron microscopy (SEM), electron crystallography and quantitative analysis, particle size analysis (PSA), surface area analysis, solubility testing, and dissolution testing. In certain embodiments, the terms "about" and "approximately" when used in this context indicate that a numerical value or range of values ​​may vary within 30%, 20%, 15%, 10%, 9%, 8%, 7%, 6%, 5%, 4%, 3%, 2%, 1.5%, 1%, 0.5%, or 0.25% of the stated value or range of values. For example, in some embodiments, the values ​​of the XRPD peak positions may vary by ±0.2 degrees 2θ (or ±0.2 degrees 2θ) and still describe a particular XRPD peak.

[0021] An "alkyl" group is a saturated, partially saturated, or unsaturated, straight- or branched-chain acyclic hydrocarbon having 1 to 10 carbon atoms, typically 1 to 8 carbons, or in some embodiments 1 to 6, 1 to 4, or 2 to 6 carbon atoms. Representative alkyl groups include -methyl, -ethyl, -n-propyl, -n-butyl, -n-pentyl, and -n-hexyl, while saturated branched-chain alkyls include -isopropyl, -sec-butyl, -isobutyl, -tert-butyl, -isopentyl, -neopentyl, -tert-pentyl, -2-methylpentyl, -3-methylpentyl, -4-methylpentyl, -2,3-dimethylbutyl, and the like. Examples of unsaturated alkyl groups include, but are not limited to, vinyl, allyl, -CH=CH(CH), -CH=C(CH), -C(CH)=CH, -C(CH)=CH(CH), -C(CHCH)=CH, -C≡CH, -C≡C(CH), -C≡C(CHCH), -CHC≡CH, -CHC≡C(CH), and -CHC≡C(CHCH), among others. Alkyl groups can be substituted or unsubstituted. When alkyl groups described herein are referred to as "substituted," they may be substituted with any substituent or substituents as found in the exemplary compounds and embodiments disclosed herein, as well as halogen (chloro, iodo, bromo, or fluoro); alkyl; hydroxyl; alkoxy; alkoxyalkyl; amino; alkylamino; carboxy; nitro; cyano; thiol; thioether; imine; imide; amidine; guanidine; enamine; aminocarbonyl; acylamino; phosphonate; phosphine; thiocarbonyl; sulfonyl; sulfone; sulfonamide; ketone; aldehyde; ester; urea; urethane; oxime; hydroxylamine; alkoxyamine; aralkoxyamine; N-oxide; hydrazine; hydrazide; hydrazone; azide; isocyanate; isothiocyanate; cyanate; thiocyanate; B(OH)2 or O(alkyl)aminocarbonyl.

[0022] An "alkenyl" group is a straight- or branched-chain acyclic hydrocarbon having 2 to 10 carbon atoms, typically 2 to 8 carbon atoms, and containing at least one carbon-carbon double bond. Representative straight- and branched-chain (C2-C8) alkenyls include -vinyl, -allyl, -1-butenyl, -2-butenyl, -isobutylenyl, -1-pentenyl, -2-pentenyl, -3-methyl-1-butenyl, -2-methyl-2-butenyl, -2,3-dimethyl-2-butenyl, -1-hexenyl, 2-hexenyl, -3-hexenyl, -1-heptenyl, -2-heptenyl, -3-heptenyl, -1-octenyl, -2-octenyl, -3-octenyl, and the like. The double bond of an alkenyl group can be unconjugated or conjugated to another unsaturated group. An alkenyl group can be unsubstituted or substituted.

[0023] An "alkynyl" group refers to a monovalent hydrocarbon radical moiety containing at least two carbon atoms and one or more carbon-carbon triple bonds. Alkynyl is optionally substituted and can be straight, branched, or cyclic. Alkynyl includes, but is not limited to, radicals having 2 to 20 carbon atoms (i.e., C 2~20 alkynyl); radicals having 2 to 12 carbon atoms (i.e., C 2~12 alkynyl); radicals having 2 to 8 carbon atoms (i.e., C 2~8 alkynyl); radicals having 2 to 6 carbon atoms (i.e., C 2~6 alkynyl); and 2 to 4 carbon atoms (i.e., C 2~4 Examples of alkynyl moieties include, but are not limited to, ethynyl, propynyl, and butynyl.

[0024] A "cycloalkyl" group is a saturated, partially saturated, or unsaturated cyclic alkyl group of 3 to 10 carbon atoms having a single ring or multiple fused or bridged rings, optionally substituted with 1 to 3 alkyl groups. In some embodiments, cycloalkyl groups have 3 to 8 ring members, while in other embodiments the number of ring carbon atoms ranges from 3 to 5, 3 to 6, or 3 to 7. Cycloalkyls containing more than one ring can be fused, spiro, or bridged cycloalkyls, or combinations thereof. Such cycloalkyl groups include, by way of example, single ring structures such as cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, cyclooctyl, 1-methylcyclopropyl, 2-methylcyclopentyl, 2-methylcyclooctyl, and the like, or multiple ring or bridged ring structures such as 1-bicyclo[1.1.1]pentyl, bicyclo[2.1.1]hexyl, bicyclo[2.2.1]heptyl, bicyclo[2.2.2]octyl, adamantyl, and the like. Examples of unsaturated cycloalkyl groups include, among others, cyclohexenyl, cyclopentenyl, cyclohexadienyl, butadienyl, pentadienyl, and hexadienyl. The cycloalkyl groups can be substituted or unsubstituted. Such substituted cycloalkyl groups include, by way of example, cyclohexanol, and the like.

[0025] A "bridged" bicyclic ring system contains two rings that share three, four, or five adjacent ring atoms. As used herein, the term "bridge" refers to an atom or chain of atoms that connects two different parts of a molecule. The two atoms (usually, but not necessarily, two tertiary carbon atoms) connected by the bridge are referred to as "bridgeheads." In addition to the bridge, the two bridgeheads are connected by at least two separate atoms or chains of atoms. Examples of bridged bicyclic ring systems include adamantanyl, norbornanyl, bicyclo[3.2.1]octyl, bicyclo[2.2.2]octyl, bicyclo[3.3.1]nonyl, bicyclo[3.2.3]nonyl, 2-oxabicyclo[2.2.2]octyl, 1-azabicyclo[2.2.2]octyl, 3-azabicyclo[3.2.1]octyl, and 2-azabicyclo[3.2.1]octyl. Examples include, but are not limited to, 6-dioxatricyclo[3.3.1.03,7]nonyl. In one embodiment, the bridge is unsubstituted or substituted -(CH2) n -, where n is 1, 2, 3, 4, or 5. In one embodiment, the bridge is -CH2-. In one embodiment, the bridge is -(CH2)2-. In one embodiment, the bridge is -(CH2)3-. In one embodiment, the bridge is -CH2-O-CH2-. "Spiro" bicyclic systems share a single ring atom (usually a quaternary carbon atom) between the two rings.

[0026] An "aryl" group is an aromatic carbocyclic group of 6 to 14 carbon atoms having a single ring (e.g., phenyl) or multiple condensed rings (e.g., naphthyl or anthryl). In some embodiments, aryl groups contain 6 to 14 carbons, and in other examples, 6 to 12 or even 6 to 10 carbon atoms in the ring portion of the group. Specific aryl groups include phenyl, biphenyl, naphthyl, and the like. Aryl groups can be substituted or unsubstituted. The term "aryl group" also encompasses groups containing fused rings, for example, fused aromatic aliphatic ring systems (e.g., indanyl, tetrahydronaphthyl, and the like).

[0027] A "heterocyclyl" is an aromatic (also referred to as heteroaryl) or non-aromatic cycloalkyl in which 1 to 4 of the ring carbon atoms are independently replaced with heteroatoms from the group consisting of O, S, and N. In some embodiments, heterocyclyl groups contain 3 to 10 ring members, while other such groups have 3 to 5, 3 to 6, or 3 to 8 ring members. A heterocyclyl may also be attached to other groups at any ring atom (i.e., at any carbon atom or heteroatom of the heterocycle). A heterocyclyl group may be substituted or unsubstituted. A heterocyclyl group may include multiple fused rings, including, but not limited to, bicyclic, tricyclic, and tetracyclic rings, as well as bridged or spiro ring systems. Heterocyclyl groups include unsaturated, partially saturated, and saturated ring systems, such as imidazolyl, imidazolinyl, and imidazolidinyl (e.g., imidazolidin-4-one or imidazolidine-2,4-dionyl) groups. The term heterocyclyl encompasses fused ring species, including those containing fused aromatic and non-aromatic groups, such as 1- and 2-aminotetralin, benzotriazolyl (e.g., 1H-benzo[d][1,2,3]triazolyl), benzimidazolyl (e.g., 1H-benzo[d]imidazolyl), 2,3-dihydrobenzo[1,4]dioxinyl, and benzo[1,3]dioxylyl. The term also encompasses bridged polycyclic ring systems containing heteroatoms, such as, but not limited to, quinuclidyl. Representative examples of heterocyclyl groups include, but are not limited to, aziridinyl, azetidinyl, azepanyl, oxetanyl, pyrrolidyl, imidazolidinyl (e.g., imidazolidin-4-onyl or imidazolidin-2,4-dionyl), pyrazolidinyl, thiazolidinyl, tetrahydrothiophenyl, tetrahydrofuranyl, dioxylyl, furanyl, thiophenyl, pyrrolyl, pyrrolinyl, imidazolyl, imidazolinyl, pyrazolyl, pyrazolinyl, triazolyl, tetrazolyl, oxazolyl, isoxazolyl, benzisoxazolyl (e.g., benzo[d]isoxazolyl), thiazolyl, thiazolinyl, isothiazolyl, thiadiazolyl, oxadiazolyl, piperidyl, piperazinyl (e.g., piperazin-2-onyl), morpholinyl nyl, thiomorpholinyl, tetrahydropyranyl (e.g., tetrahydro-2H-pyranyl), tetrahydrothiopyranyl, oxathianyl, dioxyl, dithianyl, pyranyl, pyridyl, pyrimidyl, pyridazinyl, pyrazinyl, triazinyl, dihydropyridyl, dihydrodithionyl, dihydrodithionyl, 1,4-dioxaspiro[4.5]decanyl, 2-oxo-1-oxa-3,8-diazaspiro[4.5]decane, 1-oxo-2,8-diazaspiro[4.5]decane, 3-oxo-2,8-diazaspiro[4.5]decane quinuclidyl, 3-oxo-1-oxa-4,9-diazaspiro[5.5]undecane, 2-oxo-1-oxa-3,9-diazaspiro[5.5]undecane, homopiperazinyl, quinuclidyl, indolyl (e.g., indol-2-onyl or isoindolin-1-onyl), indolinyl, isoindolyl, isoindolinyl, azaindolyl (pyrrolopyridyl or 1H-pyrrolo[2,3-b]pyridyl), indazolyl, indolizinyl, benzotriazolyl (e.g., 1H-benzo[d][1,2,3]triazolyl), benzo[d][1,2,3]triazolyl), benzo[d][1,2,3]triazolyl, ... imidazolyl (e.g., 1H-benzo[d]imidazolyl or 1H-benzo[d]imidazol-2(3H)-onyl), benzofuranyl, benzothiophenyl, benzothiazolyl, benzoxadiazolyl, benzoxazinyl, benzodithiinyl, benzoxathiinyl, benzothiazinyl, benzoxazolyl (i.e., benzo[d]oxazolyl), benzothiazolyl, benzothiadiazolyl, benzo[1,3]dioxylyl, pyrazolopyridyl (e.g., 1H-pyrazolo[3,4-b]pyridyl, 1H-pyrazolo[4,3-b]pyridyl), imidazopyridyl (e.g., azabenzimidazolyl or 1H-imidazo[4,5-b]pyridyl), triazolopyridyl, isoxazolopyridyl, purinyl, xanthinyl, adeninyl, guaninyl, quinolinyl, isoquinolinyl (e.g., 3,4-dihydroisoquinolin-1(2H)-onyl), quinolidinyl, quinoxalinyl, quinazolinyl, cinnolinyl, phthalazinyl, naphthyridinyl, pteridinyl, thianaphthalenyl, Included are dihydrobenzothiazinyl, dihydrobenzofuranyl, dihydroindolyl, dihydrobenzodioxinyl, tetrahydroindolyl, tetrahydroindazolyl, tetrahydrobenzimidazolyl, tetrahydrobenzotriazolyl, tetrahydropyrrolopyridyl, tetrahydropyrazolopyridyl, tetrahydroimidazopyridyl, tetrahydrotriazolopyridyl, tetrahydropyrimidin-2(1H)-one, and tetrahydroquinolinyl groups. Representative non-aromatic heterocyclyl groups do not include fused ring species containing fused aromatic groups. Examples of non-aromatic heterocyclyl groups include aziridinyl, azetidinyl, azepanyl, pyrrolidyl, imidazolidinyl (e.g., imidazolidin-4-onyl or imidazolidin-2,4-dionyl), pyrazolidinyl, thiazolidinyl, tetrahydrothiophenyl, tetrahydrofuranyl, piperidyl, piperazinyl (e.g., piperazin-2-onyl), morpholinyl, thiomorpholinyl, tetrahydropyranyl (e.g., tetrahydro-2H-pyranyl), tetrahydrothiopyranyl, oxathianyl, dithianyl, 1,4-dioxaspiro[4.5]decanyl, homopiperazinyl, quinuclidyl, or tetrahydropyrimidin-2(1H)-one. Representative substituted heterocyclyl groups may be mono-substituted or more than twice substituted, for example, pyridyl or morpholinyl groups substituted at the 2-, 3-, 4-, 5-, or 6-position with various substituents, such as, but not limited to, those listed below, or di-substituted.

[0028] A "heteroaryl" group is an aryl ring system having 1 to 4 heteroatoms as ring atoms in a heteroaromatic ring system, with the remaining atoms being carbon atoms. In some embodiments, heteroaryl groups contain 3 to 6 ring atoms, and in other examples, 6 to 9 or even 6 to 10 atoms in the ring portion of the group. Suitable heteroatoms include oxygen, sulfur, and nitrogen. In certain embodiments, heteroaryl ring systems are monocyclic or bicyclic. Non-limiting examples include, but are not limited to, pyrrolyl, pyrazolyl, imidazolyl, triazolyl, tetrazolyl, oxazolyl, isoxazolyl, benzisoxazolyl (e.g., benzo[d]isoxazolyl), thiazolyl, pyrrolyl, pyridazinyl, pyrimidyl, pyrazinyl, thiophenyl, benzothiophenyl, furanyl, benzofuranyl, indolyl (e.g., indolyl-2-onyl or isoindolin-1-onyl), azaindolyl (pyrrolopyridyl or 1H-pyrrolo[2,3-b]pyridyl), indazolyl, benzimidazolyl (e.g., 1H-benzo[d]imidazolyl), isopropyl, ... Included are groups such as midazopyridyl (e.g., azabenzimidazolyl or 1H-imidazo[4,5-b]pyridyl), pyrazolopyridyl, triazolopyridyl, benzotriazolyl (e.g., 1H-benzo[d][1,2,3]triazolyl), benzoxazolyl (e.g., benzo[d]oxazolyl), benzothiazolyl, benzothiadiazolyl, isoxazolopyridyl, thianaphthalenyl, purinyl, xanthinyl, adeninyl, guaninyl, quinolinyl, isoquinolinyl (e.g., 3,4-dihydroisoquinolin-1(2H)-onyl), tetrahydroquinolinyl, quinoxalinyl, and quinazolinyl groups.

[0029] As used herein, a "spirocycle" refers to two or more rings in which adjacent rings are joined through a single atom. The individual rings within a spirocycle may be the same or different. The individual rings within a spirocycle may be substituted or unsubstituted and may have different substituents than the other individual rings within a set of spirocycles.

[0030] A "cycloalkylalkyl" group is a radical of the formula: -alkyl-cycloalkyl, where alkyl and cycloalkyl are as defined above. Substituted cycloalkylalkyl groups can be substituted on the alkyl, cycloalkyl, or both the alkyl and cycloalkyl portions of the group. Representative cycloalkylalkyl groups include, but are not limited to, methylcyclopropyl, methylcyclobutyl, methylcyclopentyl, methylcyclohexyl, ethylcyclopropyl, ethylcyclobutyl, ethylcyclopentyl, ethylcyclohexyl, propylcyclopentyl, propylcyclohexyl, and the like.

[0031] An "aralkyl" group is a radical of the formula: -alkyl-aryl, where alkyl and aryl are as defined above. Substituted aralkyl groups can be substituted on the alkyl, the aryl, or both the alkyl and aryl portions of the group. Representative aralkyl groups include, but are not limited to, benzyl and phenethyl groups and fused (cycloalkylaryl)alkyl groups, such as 4-ethyl-indanyl.

[0032] A "heterocyclylalkyl" group is a radical of the formula: -alkyl-heterocyclyl, where alkyl and heterocyclyl are defined above. Substituted heterocyclylalkyl groups can be substituted on the alkyl, the heterocyclyl, or both the alkyl and the heterocyclyl portions of the group. Representative heterocyclylalkyl groups include, but are not limited to, 4-ethylmorpholinyl, 4-propylmorpholinyl, furan-2-ylmethyl, furan-3-ylmethyl, pyridin-3-ylmethyl, tetrahydrofuran-2-ylethyl, and indol-2-ylpropyl.

[0033] "Halogen" is fluorine, chlorine, bromine, or iodine.

[0034] A "hydroxyalkyl" group is an alkyl group, as defined above, that is substituted with one or more hydroxy groups.

[0035] An "alkoxy" or "alkoxyl" group is an --O-(alkyl), where alkyl is as defined above.

[0036] An "alkoxyalkyl" group is an -(alkyl)-O-(alkyl), where alkyl is as defined above.

[0037] An "amino" group is a radical of the formula: --NH.sub.2.

[0038] An "alkylamino" group is a radical of the formula: --NH-alkyl or --N(alkyl)2, where each alkyl is independently as defined above.

[0039] A "carboxy" group is a radical of the formula: --C(O)OH.

[0040] An “aminocarbonyl” group is a group of the formula: —C(O)N(R # )2, -C(O)NH(R # ), or a group of —C(O)NH, where each R # is independently a substituted or unsubstituted alkyl, cycloalkyl, aryl, aralkyl, heterocyclyl, or heterocyclyl group as described herein.

[0041] An "acylamino" group is a group of the formula: -NHC(O)(R # ) or -N(alkyl)C(O)(R # ) where each alkyl and R # are independently as defined above.

[0042] A "sulfonylamino" group is a group of the formula: -NHSO(R # ) or -N(alkyl)SO2(R # ) where each alkyl and R # is defined above.

[0043] A "urea" group is a group of the formula: -N(alkyl)C(O)N(R #)2, -N(alkyl)C(O)NH(R # ), -N(alkyl)C(O)NH2, -NHC(O)N(R # )2, -NHC(O)NH(R # ), or -NH(CO)NHR # where each alkyl and R # are independently as defined above.

[0044] When groups described herein, except for alkyl groups, are referred to as "substituted," they may be substituted with any suitable substituent or substituents. Illustrative examples of substituents include those found in the exemplary compounds and embodiments disclosed herein, as well as halogen (chloro, iodo, bromo, or fluoro); alkyl; hydroxyl; alkoxy; alkoxyalkyl; amino; alkylamino; carboxy; nitro; cyano; thiol; thioether; imine; imide; amidine; guanidine; enamine; aminocarbonyl; acylamino; phosphonate; phosphine; thiocarbonyl; sulfonyl; sulfone; sulfonamide; ketone; aldehyde; ester; urea; urethane; oxime; hydroxylamine; alkoxyamine; aralkoxyamine; N-oxide; hydrazine; hydrazide; hydrazone; azide; isocyanate; isothiocyanate; cyanate; thiocyanate; oxygen (=O); B(OH), O(alkyl)aminocarbonyl; monocyclic or fused polycyclic rings. cycloalkyl, which may be monocyclic or non-fused polycyclic (e.g., cyclopropyl, cyclobutyl, cyclopentyl, or cyclohexyl), or heterocyclyl, which may be monocyclic, fused polycyclic, or non-fused polycyclic (e.g., pyrrolidyl, piperidyl, piperazinyl, morpholinyl, or thiazinyl); monocyclic, fused polycyclic, or non-fused polycyclic aryl or heteroaryl (e.g., phenyl, naphthyl, pyrrolyl, indolyl, furanyl, thiophenyl, imidazolyl, oxazolyl, isoxazolyl, thiazolyl, triazolyl, tetrazolyl, pyrazolyl, pyridyl, quinolinyl, isoquinolinyl, acridinyl, pyrazinyl, pyridazinyl, pyrimidyl, benzimidazolyl, benzothiophenyl, or benzofuranyl); aryloxy; aralkyloxy; heterocyclyloxy; and heterocyclylalkoxy.

[0045] As used herein, the term "pharmaceutically acceptable salt(s)" refers to salts prepared from pharmaceutically acceptable non-toxic acids or bases, including inorganic acids and inorganic bases, and organic acids and organic bases. Suitable pharmaceutically acceptable base addition salts of compounds of formula (I) include, but are not limited to, those well known in the art. For example, see Remington's Pharmaceutical Sciences, 18 th eds., Mack Publishing, Easton PA (1990), or Remington: The Science and Practice of Pharmacy, 19 th eds., Mack Publishing, Easton PA (1995).

[0046] As used herein, and unless otherwise specified, the term "stereoisomer" or "stereoisomerically pure" refers to one stereoisomer of a compound that is substantially free of other stereoisomers of that compound. For example, a stereoisomerically pure compound having one chiral center is substantially free of the opposite enantiomer of the compound. A stereoisomerically pure compound having two chiral centers is substantially free of other diastereoisomers of the compound. A typical stereoisomerically pure compound contains greater than about 80% by weight of one stereoisomer of the compound and less than about 20% by weight of other stereoisomers of the compound, greater than about 90% by weight of one stereoisomer of the compound and less than about 10% by weight of other stereoisomers of the compound, greater than about 95% by weight of one stereoisomer of the compound and less than about 5% by weight of other stereoisomers of the compound, or greater than about 97% by weight of one stereoisomer of the compound and less than about 3% by weight of other stereoisomers of the compound. The compounds may have chiral centers and may occur as racemates, individual enantiomers or diastereoisomers, and mixtures thereof, and all such isomeric forms, including mixtures thereof, are encompassed within the scope of the embodiments disclosed herein.

[0047] The use of such compounds in stereomerically pure forms, as well as mixtures of these forms, are encompassed by the embodiments disclosed herein. For example, mixtures containing equal or unequal amounts of enantiomers of a particular compound may be used in the methods and compositions disclosed herein. These isomers may be asymmetrically synthesized or resolved using standard techniques, such as chiral columns or chiral resolving agents. See, for example, Jacques, J., et al., Enantiomers, Racemates and Resolutions (Wiley-Interscience, New York, 1981); Wilen, SH, et al., Tetrahedron 33:2725 (1977); Eliel, EL, Stereochemistry of Carbon Compounds (McGraw-Hill, NY, 1962); and Wilen, SH, Tables of Resolving Agents and Optical Resolutions p. 268 (EL Eliel, Ed., University of Notre Dame Press, Notre Dame, IN, 1972).

[0048] It should also be noted that the compounds may include E and Z isomers or mixtures thereof, as well as cis and trans isomers or mixtures thereof. In certain embodiments, the compounds are isolated as either the E or Z isomer. In other embodiments, the compounds are a mixture of E and Z isomers.

[0049] As used herein, and unless otherwise specified, "atropisomer" refers to a stereoisomer resulting from hindrance of rotation about a single bond axis, where the rotational barrier is high enough to permit isolation of individual rotamers.

[0050] "Tautomer" refers to isomeric forms of a compound that are in equilibrium with each other. The concentration of isomeric forms depends on the environment in which the compound is found, and may vary depending on, for example, whether the compound is solid or in an organic or aqueous solution. For example, in aqueous solution, pyrazole may exhibit the following isomeric forms, which are referred to as tautomers with each other: [ka]

[0051] As will be readily understood by one of ordinary skill in the art, a wide variety of functional groups and other structures may exhibit tautomerism, and all tautomers of the compounds of formula (I) are within the scope of the invention.

[0052] It is also noted that the compounds may contain unnatural proportions of atomic isotopes at one or more atoms. For example, the compounds may contain radioactive isotopes, such as tritium ( 3 H), iodine-125( 125 I), sulfur 35( 35 S), or carbon-14 ( 14 C), or may be radiolabeled with, for example, deuterium ( 2 H), carbon-13 ( 13 C), or nitrogen-15( 15N) may be isotopically enriched. As used herein, an "isotopically enriched" compound is an isotopically enriched compound. The term "isotopically enriched" refers to an atom having an isotopic composition other than the natural isotopic composition of that atom. "Isotopically enriched" can also refer to a compound containing at least one atom having an isotopic composition other than the natural isotopic composition of that atom. The term "isotopic composition" refers to the amount of each isotope present for a given atom. Radiolabeled and isotopically enriched compounds are useful as therapeutic agents, e.g., cancer and inflammation therapeutic agents, research reagents, e.g., binding assay reagents, and diagnostic agents, e.g., in vivo imaging agents. All isotopic variations of the compounds described herein, whether radioactive or not, are intended to be encompassed within the scope of the embodiments provided herein. In some embodiments, isotopically enriched compounds are provided, e.g., compounds enriched in deuterium, carbon-13, or nitrogen-15.

[0053] As used herein, "treating" refers to the total or partial alleviation of a disorder, disease, or condition, or one or more symptoms associated with the disorder, disease, or condition, or the slowing or halting of further progression or worsening of those symptoms, or the reduction or eradication of the cause(s) of the disorder, disease, or condition itself. In some embodiments, "treating" refers to the total or partial alleviation of a disorder, disease, or condition, or the slowing or halting of further progression or worsening of those symptoms. In another embodiment, "treating" refers to the total or partial alleviation of a disorder, disease, or condition, or symptoms associated with a condition, wherein the condition is treatable or preventable by inhibition of KRAS, preferably G12D and / or G12V.

[0054] As used herein, "preventing" means a method of delaying and / or preventing, in whole or in part, the onset, recurrence, or spread of a disorder, disease, or condition; a method of preventing a subject from acquiring a disorder, disease, or condition; or a method of reducing a subject's risk of acquiring a disorder, disease, or condition. In one embodiment, the condition is one that is treatable or preventable by inhibition of KRAS, preferably G12D and / or G12V.

[0055] The term "effective amount" in connection with a compound means an amount capable of treating or preventing a disorder, disease, or condition disclosed herein, or a symptom thereof.

[0056] The term "subject" encompasses animals, including, but not limited to, animals such as cows, monkeys, horses, sheep, pigs, chickens, turkeys, quail, cats, dogs, mice, rats, rabbits, or guinea pigs, and in one embodiment, mammals, and in another embodiment, humans.

[0057] compound Aspect 1: Formula (I): [ka] and pharmaceutically acceptable salts, tautomers, stereoisomers, enantiomers, atropisomers, isotopically substituted derivatives, and prodrugs thereof, During the ceremony, Ring A is unsubstituted or substituted aryl, or unsubstituted or substituted heteroaryl; Ring B is unsubstituted or substituted cycloalkyl or unsubstituted or substituted heterocyclyl; X is N or CR 8 and R 0 each independently represents H, halogen, amino, —CN, —OH, unsubstituted or substituted C 1~4 Alkyl, unsubstituted or substituted C 1~4 Alkenyl, unsubstituted or substituted C 1~4Alkynyl, unsubstituted or substituted C 1~4 Alkoxy, unsubstituted or substituted C 3~5 Cycloalkyl, 3- to 5-membered unsubstituted or substituted heterocyclyl, unsubstituted or substituted C 1~4 alkylamino, carboxy, nitro, thiol, or thioether, or R 0 one or more pairs of groups, together with the atoms to which they are attached, form an unsubstituted or substituted cycloalkyl, unsubstituted or substituted heterocyclyl, unsubstituted or substituted aryl, or unsubstituted or substituted heteroaryl; R 3a , R 3b , R 4a , R 4b , R 5 , R 6a , R 6b , R 7a , and R 7b each independently represents H, halogen, unsubstituted or substituted amino, —CN, —OH, unsubstituted or substituted C 1~4 Alkyl, unsubstituted or substituted C 1~4 Alkoxy, unsubstituted or substituted C 3~5 Cycloalkyl, 3- to 5-membered unsubstituted or substituted heterocyclyl, unsubstituted or substituted C 1~4 alkylamino, carboxy, nitro, thiol, or thioether, and optionally R 3a and R 3b together with the atom to which they are attached form an unsubstituted or substituted cycloalkyl or an unsubstituted or substituted heterocyclyl, or R 4a and R 4b together with the atom to which they are attached form an unsubstituted or substituted cycloalkyl or an unsubstituted or substituted heterocyclyl, or R 6a and R 6b together with the atom to which they are attached form an unsubstituted or substituted cycloalkyl or an unsubstituted or substituted heterocyclyl, or R 7a and R 7btogether with the atom to which they are attached form an unsubstituted or substituted cycloalkyl, or an unsubstituted or substituted heterocyclyl, or optionally R 3a and R 4a together with the atom to which they are attached form an unsubstituted or substituted cycloalkyl or an unsubstituted or substituted heterocyclyl, or R 4a and R 5 together with the atom to which they are attached form an unsubstituted or substituted cycloalkyl or an unsubstituted or substituted heterocyclyl, or R 5 and R 6a together with the atom to which they are attached form an unsubstituted or substituted cycloalkyl or an unsubstituted or substituted heterocyclyl, or R 6a and R 7a together with the atom to which they are attached form an unsubstituted or substituted cycloalkyl, or an unsubstituted or substituted heterocyclyl; R 8 is H, halogen, unsubstituted or substituted C 1~4 Alkyl, unsubstituted or substituted C 1~4 Alkenyl, unsubstituted or substituted C 3~5 Cycloalkyl, unsubstituted or substituted C 1~4 Alkoxy, unsubstituted or substituted C 1~4 Alkyl halides, unsubstituted or substituted C 3~5 Halogenated cycloalkyl, unsubstituted or substituted C 1~4 halogenated alkoxyl, CN, OH, or amino; t is 0 or 1, u is 1, 2, 3 or 4, each of m and q is independently an integer between 0 and the maximum number of substituents allowed on rings A and B, respectively; If u is 2, 3, or 4, then each R 6a are independent of each other, and each R 6bare independent of each other, and pharmaceutically acceptable salts, tautomers, stereoisomers, enantiomers, atropisomers, isotopic substitutions, and prodrugs thereof are provided herein.

[0058] In some embodiments, m is an integer from 0 to 5. In some embodiments, m is an integer from 1 to 4. In some embodiments, m is an integer from 2 to 3. In some embodiments, m is an integer of 2 or 3. In some embodiments, q is an integer from 0 to 5. In some embodiments, q is an integer from 1 to 4. In some embodiments, q is an integer from 1 to 3. In some embodiments, q is an integer of 1 or 2.

[0059] In one embodiment, when u is 2, 3, or 4, R 6a and R 6b Two of the groups may form a ring or spiro ring.

[0060] Aspect 2:

[0061] In one embodiment, ring A is an aryl group (eg, phenyl or naphthyl) optionally substituted with one or more substituents. In one embodiment, a substituent is F, Cl, Br, amino, -CN, OH, -CF, -CHF, -CHF, -CFCH, -CFCF, -OCHF, -OCF, vinyl (-CH=CH), -propylenyl (e.g., -C(CH)=CH), -CF=CH, aryl, heteroaryl, ethynyl, propynyl, butynyl, pentynyl, hexynyl, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, cyclooctyl, methyl, ethyl, propyl, butyl, pentyl, hexyl, heptyl, octyl, vinyl, propylenyl, allyl, butenyl, butadienyl, pentenyl, pentadienyl, hexenyl, hexadienyl, methoxy, ethoxy, propoxy, isopropoxy, butoxy, tert-butoxy, pentoxy, or hexoxy. In one embodiment, the substituents are F, Cl, Br, amino, -CN, OH, -CF3, -CHF2, -CH2F, -CF2CH3, -CF2CF3, -OCHF2, -OCF3, vinyl (-CH=CH), -propylenyl (e.g., -C(CH3)=CH), -CF=CH, aryl, heteroaryl, ethynyl, propynyl, butynyl, pentynyl, hexynyl, cyclopropyl, methylcyclopropyl, fluorocyclopropyl, difluorocyclopropyl. cyclopropyl, fluoromethylcyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, cyclooctyl, methyl, ethyl, propyl, butyl, pentyl, hexyl, heptyl, octyl, vinyl, propylenyl, allyl, butenyl, butadienyl, pentenyl, pentadienyl, hexenyl, hexadienyl, methoxy, ethoxy, propoxy, isopropoxy, butoxy, tert-butoxy, pentoxy, or hexoxy.

[0062] In one embodiment, Ring A is a 5- to 7-membered monocyclic heteroaryl or 8- to 12-membered bicyclic heteroaryl group optionally substituted with one or more substituents. In some preferred embodiments, Ring A is pyridyl, benzothiazolyl, quinolinyl, isoquinolinyl, pyrazolopyridinyl, benzimidazolyl, quinazolinyl, or quinazolinyl. In one embodiment, the substituents are F, Cl, Br, C(CH3)=CH, or -CH=CH, -CF=CH, -CN, OH, -NH2-CF3, -CHF2, -CH2F, -CF2CH3, -CF2CF3, -OCHF2, -OCF3, aryl, ethynyl, propynyl, butynyl, pentynyl, hexynyl, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, cyclooctyl, methyl, ethyl, propyl, butyl, pentyl, hexyl, heptyl, octyl, vinyl, propylenyl, allyl, butenyl, butadienyl, pentenyl, pentadienyl, hexenyl, hexadienyl, methoxy, ethoxy, propoxy, isopropoxy, butoxy, tert-butoxy, pentoxy, or hexoxy. In one embodiment, the substituents are F, Cl, Br, C(CH3)=CH, or -CH=CH, -CF=CH, -CN, OH, -NH2, -CF3, -CHF2, -CH2F, -CF2CH3, -CF2CF3, -OCHF2, -OCF3, aryl, ethynyl, propynyl, butynyl, pentynyl, hexynyl, cyclopropyl, methylcyclopropyl, fluorocyclopropyl, difluorocyclopropyl, fluoromethylcyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, cyclooctyl, methyl, ethyl, propyl, butyl, pentyl, hexyl, heptyl, octyl, vinyl, propylenyl, allyl, butenyl, butadienyl, pentenyl, pentadienyl, hexenyl, hexadienyl, methoxy, ethoxy, propoxy, isopropoxy, butoxy, tert-butoxy, pentoxy, or hexoxy. In some preferred embodiments, Ring A is pyridyl, benzothiazolyl, quinolinyl, isoquinolinyl, pyrazolopyridinyl, benzimidazolyl, quinazolinyl, or quinazolinyl.

[0063] In one embodiment, ring A is [ka] is.

[0064] In one embodiment, ring A is [ka] is.

[0065] In one embodiment, ring A is [ka] is.

[0066] In one embodiment, ring A is [ka] is.

[0067] Aspect 3:

[0068] In one embodiment, Ring B is unsubstituted or substituted cycloalkyl or unsubstituted or substituted heterocyclyl. In one embodiment, the heterocyclyl contains at least one oxygen ring member. In one embodiment, the heterocyclyl contains at least one nitrogen ring member.

[0069] In one embodiment, Ring B is cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, cyclooctyl, a 3-9 membered heterocycle containing 1, 2 or 3 nitrogen atoms as ring members. In one embodiment, Ring B is oxazolidinyl, imidazolidinyl, thiazolidinyl, pyrazolidinyl, morpholinyl, thiomorpholinyl, piperidinyl, piperazinyl, oxazinyl, imidazolyl, thiazolyl, oxazolyl, pyridyl, pyrimidinyl, pyrazinyl, pyridazinyl, phenyl, pyrrolyl, imidazolyl, pyrazolyl, thiazolyl, oxazolyl, triazolyl, thiophenyl, furanyl, pyridyl, pyrimidinyl, pyrazinyl, phenyl, tetrahydropyridinyl, azetidinyl, pyrrolidinyl, octahydroindolizinyl, octahydroquinolidinyl, hexahydro-1H-pyrrolidinyl, tetrahydroisoquinolinyl, or tetrahydropyridyl, wherein Ring B is optionally substituted. In one embodiment, Ring B is oxetanyl, tetrahydrofuryl, tetrahydro-2H-pyranyl, dihydro-2H-pyranyl, oxabicyclo[2.1.1]hexyl, oxabicyclo[2.2.1]heptyl, oxaspiro[3.3]heptyl, oxabicyclo[3.2.1]octyl, oxabicyclo[2.2.2]octyl, oxaspiro[3.5]nonyl, or oxaspiro[3.4]octyl, wherein Ring B is optionally substituted. In one embodiment, Ring B is optionally substituted with halogen, cyano, hydroxy, alkoxy, or alkyl optionally substituted with halogen, cyano, hydroxy, alkoxy, heterocyclyl, cycloalkyl, or cycloalkyloxy.

[0070] In one embodiment, Ring B is cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, cyclooctyl, a 3-9 membered heterocycle containing 1, 2, or 3 nitrogen atoms as ring members, oxazolidinyl, imidazolidinyl, thiazolidinyl, pyrazolidinyl, morpholinyl, thiomorpholinyl, piperidinyl, piperazinyl, oxazinyl, imidazolyl, thiazolyl, oxazolyl, pyridyl, pyrimidinyl, pyrazinyl, pyridazinyl, phenyl, pyrrolyl, imidazolyl, pyrazolyl, thiazolyl, oxazolyl, triazolyl, thiophenyl, furanyl, pyridyl, pyrimidinyl, pyrazinyl, phenyl, or tetrahydropyridinyl, optionally substituted with one or more substituents. In one embodiment, the substituents are methyl, ethyl, propyl, butyl, pentyl, hexyl, heptyl, -C 2~8 Alkenyl, -C 2~8 Alkynyl, C 1~8 Alkoxy-C 1~8 alkyl-, octyl, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, cyclooctyl, oxazolidinyl, imidazolidinyl, thiazolidinyl, pyrazolidinyl, morpholinyl, piperidinyl, piperazinyl, oxazinyl, imidazolyl, thiazolyl, oxazolyl, pyridyl, pyrimidinyl, pyrazinyl, pyridazinyl, phenyl, pyrrolyl, imidazolyl, pyrazolyl, thiazolyl, oxazolyl, triazolyl, thiophenyl, furanyl, pyridyl, pyrimidinyl, pyrazinyl, or oxo; or two substituents, together with the carbon atoms to which they are attached, form a 3-8 membered unsaturated or saturated ring containing 0, 1, 2, or 3 heteroatoms independently selected from nitrogen, oxygen, or sulfur.

[0071] In one embodiment, ring B is [ka] is.

[0072] In one embodiment, Ring B is azetidyl, pyridyl, isoxazolyl, oxazolyl, dihydro-2H-pyranyl, tetrahydro-2H-pyranyl, pyrrolidinonyl, azaspiro[3.3]heptyl, azabicyclo[2.1.1]hexyl, pyrrolidyl, 1H-pyrazolyl, wherein Ring B is optionally substituted.

[0073] In one embodiment, Ring B is optionally substituted with halogen, cyano, hydroxy, alkoxy, or alkyl optionally substituted with halogen, cyano, hydroxy, or alkoxy.

[0074] In one embodiment, ring B is [ka] is.

[0075] In one embodiment, Ring B is azetidyl, pyridyl, isoxazolyl, oxazolyl, dihydro-2H-pyranyl, tetrahydro-2H-pyranyl, pyrrolidinonyl, azaspiro[3.3]heptyl, azabicyclo[2.1.1]hexyl, pyrrolidyl, 1H-pyrazolyl, wherein Ring B is optionally substituted.

[0076] In one embodiment, Ring B is cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, or azetidyl, wherein Ring B is optionally substituted.

[0077] In one embodiment, ring B is [ka] is.

[0078] In one embodiment, ring B is [ka] is.

[0079] In one embodiment, ring B is [ka] is.

[0080] Aspect 4:

[0081] In one embodiment, ring A is substituted or unsubstituted phenyl, substituted or unsubstituted naphthyl, substituted or unsubstituted benzo[b]thiophenyl, or substituted or unsubstituted benzo[d]thiazolyl.

[0082] In one embodiment, ring A is [ka] is.

[0083] In one embodiment, ring A is [ka] is.

[0084] Aspect 5:

[0085] In one embodiment, Ring B is substituted or unsubstituted hexahydro-1H-pyrrolidinyl, substituted or unsubstituted pyrrolidinyl, substituted or unsubstituted aminomethylcyclopropyl, substituted or unsubstituted oxetanyl, substituted or unsubstituted tetrahydrofuranyl, substituted or unsubstituted oxabicyclo[2.1.1]hexanyl, substituted or unsubstituted oxabicyclo[2.2.1]heptanyl.

[0086] In one embodiment, ring B is [ka] where R a and R b each independently represents a substituted or unsubstituted C 1~4 Alkyl, substituted or unsubstituted C 3~5 cycloalkyl or R a and R btogether with the N to which they are attached form a substituted or unsubstituted heterocycle containing N, O, or S; R c is H, halogen, CN, substituted or unsubstituted C 1~4 Alkyl, substituted or unsubstituted C 3~5 Cycloalkyl, substituted or unsubstituted C 1~4 It is alkoxyl.

[0087] In one embodiment, ring B is [ka] In one embodiment, ring B is [ka] In one embodiment, ring B is [ka] In one embodiment, ring B is [ka] In one embodiment, ring B is [ka] In one embodiment, ring B is [ka] In one embodiment, ring B is [ka] In one embodiment, ring B is [ka] In one embodiment, ring B is [ka] is.

[0088] Aspect 6:

[0089] In one embodiment, X is N. In one embodiment, X is CR 8 In one embodiment, X is CH, CF, C—Cl, or C—CF. In one embodiment, X is C—Cl. In one embodiment, X is C—CF.

[0090] Aspect 7:

[0091] Group 1: In one embodiment, t is 0.

[0092] Group 1.1: In one embodiment, u is 1. In one embodiment, the compound has formula (II): [ka] It is a compound having the formula:

[0093] In one embodiment, R 3a , R 3b , R 5 , R 7a , and R 7b are each independently H or methyl, and R a1 , R6 b1 are independently selected from H, halogen, methyl, —CN, —OH, substituted or unsubstituted amino.

[0094] In one embodiment, ring B is [ka] where R a and R b each independently represents a substituted or unsubstituted C 1~4 Alkyl, substituted or unsubstituted C 3~5 cycloalkyl or R a and R b together with the N to which they are attached form a substituted or unsubstituted heterocycle containing N, O, or S; R cis H, halogen, CN, substituted or unsubstituted C 1~4 Alkyl, substituted or unsubstituted C 3~5 Cycloalkyl, substituted or unsubstituted C 1~4 is alkoxyl, and R 3a , R 3b , R 5 , R 7a , and R 7b is independently H or methyl, and R 6a1 , R 6b1 are independently selected from H, halogen, methyl, —CN, —OH, substituted or unsubstituted amino.

[0095] Group 1.1.1: In one embodiment, X is N.

[0096] In one embodiment, ring A is [ka] and ring B is [ka] is.

[0097] Group 1.1.1.1: In one embodiment, ring A is [ka] is.

[0098] In one embodiment, ring B is [ka] is.

[0099] Group 1.1.1.1.1: In one embodiment, ring B is [ka] In one embodiment, ring B is [ka] is.

[0100] In one embodiment, R 3a , R 3b , R 5 , R 6a1 , R 6b1 , R 7a , and R 7b each independently represents H, OH, CN, unsubstituted or substituted amino, or unsubstituted or substituted C 1~4 In one embodiment, R 3a , R 3b , R 5 , R 6a1 , R 6b1 , R 7a , and R 7b each independently represents H, or an unsubstituted or substituted C 1~4 In one embodiment, R 3a , R 3b , R 5 , R 6a1 , R 6b1 , R 7a , and R 7b Each of R is independently H, unsubstituted or substituted methyl, or unsubstituted or substituted ethyl. 3a , R 3b , R 5 , R 6a1 , R 6b1 , R 7a , and R 7b Each of R is independently methyl or ethyl optionally substituted with H, OH, CN, amino, or methylamino. 3a , R 3b , R 5 , R 6a1 , R 6b1 , R 7a , and R 7b Each of is independently H or methyl.

[0101] In one embodiment, the compound is [ka] is.

[0102] In one embodiment, R 6a1 and R 6b1 together with the atoms to which they are attached form an unsubstituted or substituted heterocyclyl. In one embodiment, the heterocyclyl is oxetanyl, tetrahydrofuranyl, tetrahydropyranyl, or dioxanyl. In one embodiment, the heterocyclyl is oxetanyl or tetrahydrofuranyl. In one embodiment, the heterocyclyl is oxetanyl. In one embodiment, the heterocyclyl is tetrahydrofuranyl.

[0103] In one embodiment, the compound is [ka] is.

[0104] Group 1.1.1.4: In one embodiment, ring A is [ka] is.

[0105] In one embodiment, R 3a , R 3b , R 5 , R 7a , and R 7b are each independently H or methyl, and R a1 , R6 b1 are independently selected from H, halogen, methyl, —CN, —OH, substituted or unsubstituted amino.

[0106] In one embodiment, ring A is [ka] and ring B is [ka] where R a and R b each independently represents a substituted or unsubstituted C 1~4Alkyl, substituted or unsubstituted C 3~5 cycloalkyl or R a and R b together with the N to which they are attached form a substituted or unsubstituted heterocycle containing N, O, or S; R c is H, halogen, CN, substituted or unsubstituted C 1~4 Alkyl, substituted or unsubstituted C 3~5 Cycloalkyl, substituted or unsubstituted C 1~4 is alkoxyl, and R 3a , R 3b , R 5 , R 7a , and R 7b is independently H or methyl, and R 6a1 , R 6b1 are independently selected from H, halogen, methyl, —CN, —OH, substituted or unsubstituted amino.

[0107] In one embodiment, ring B is [ka] is.

[0108] Group 1.1.1.4.1: In one embodiment, ring B is [ka] In one embodiment, ring B is [ka] is.

[0109] In one embodiment, R 3a , R 3b , R 5 , R 6a1 , R 6b1 , R 7a , and R 7b each independently represents H, OH, CN, amino, or unsubstituted or substituted C 1~4 In one embodiment, R 3a , R3b , R 5 , R 6a1 , R 6b1 , R 7a , and R 7b each independently represents H, or an unsubstituted or substituted C 1~4 In one embodiment, R 3a , R 3b , R 5 , R 6a1 , R 6b1 , R 7a , and R 7b Each of R is independently H, unsubstituted or substituted methyl, or unsubstituted or substituted ethyl. 3a , R 3b , R 5 , R 6a1 , R 6b1 , R 7a , and R 7b Each of R is independently methyl or ethyl optionally substituted with H, OH, CN, amino, or methylamino. 3a , R 3b , R 5 , R 6a1 , R 6b1 , R 7a , and R 7b Each of is independently H or methyl.

[0110] In one embodiment, the compound is [ka] is.

[0111] Group 1.2: In one embodiment, u is 2. In one embodiment, the compound has formula (III): [ka] It is a compound having the formula:

[0112] In one embodiment, R 3a , R 3b , R 5 , R 6a1 , R 6b1, R 6a2 , R 6b2 , R 7a , and R 7b each is independently H, halogen, OH, CN, substituted or unsubstituted amino, or methyl, or optionally, R 3a and R 5 together with the atom to which they are attached form an unsubstituted or substituted cycloalkyl or an unsubstituted or substituted heterocyclyl, or R 6a1 and R 5 together with the atom to which they are attached form an unsubstituted or substituted cycloalkyl or an unsubstituted or substituted heterocyclyl, or R 6a1 and R 6a2 together with the atom to which they are attached form an unsubstituted or substituted cycloalkyl or an unsubstituted or substituted heterocyclyl, or R 6a2 and R 7a together with the atom to which they are attached form an unsubstituted or substituted cycloalkyl, or an unsubstituted or substituted heterocyclyl.

[0113] In one embodiment, ring B is [ka] where R a and R b each independently represents a substituted or unsubstituted C 1~4 Alkyl, substituted or unsubstituted C 3~5 cycloalkyl or R a and R b together with the N to which they are attached form a substituted or unsubstituted heterocycle containing N, O, or S; R c is H, halogen, CN, substituted or unsubstituted C 1~4 Alkyl, substituted or unsubstituted C 3~5 Cycloalkyl, substituted or unsubstituted C 1~4 is alkoxyl, and R 3a , R 3b , R 5 , R 6a1, R 6b1 , R 6a2 , R 6b2 , R 7a , and R 7b Each of is independently H, halogen, OH, CN, substituted or unsubstituted amino, or methyl.

[0114] Group 1.2.1: In one embodiment, X is N.

[0115] In one embodiment, ring A is [ka] and ring B is [ka] is.

[0116] Group 1.2.1.1: In one embodiment, ring A is [ka] is.

[0117] In one embodiment, ring B is [ka] is.

[0118] Group 1.2.1.1.1: In one embodiment, ring B is [ka] In one embodiment, ring B is [ka] is.

[0119] In one embodiment, R 3a , R 3b , R 5 , R 6a1 , R 6b1 , R 6a2, R 6b2 , R 7a , and R 7b each independently represents H, halogen, OH, CN, unsubstituted or substituted amino, or unsubstituted or substituted C 1~4 In one embodiment, R 3a , R 3b , R 5 , R 6a1 , R 6b1 , R 6a2 , R 6b2 , R 7a , and R 7b Each of R is independently H, halogen, OH, CN, methylamino, methyl or ethyl optionally substituted with OH, CN, amino. 3a , R 3b , R 5 , R 6a1 , R 6b1 , R 6a2 , R 6b2 , R 7a , and R 7b is independently H, halogen, OH, CN, methylamino, or methyl.

[0120] In one embodiment, the compound is [ka] is.

[0121] Group 1.2.1.4: In one embodiment, ring A is [ka] is.

[0122] In one embodiment, R 3a , R 3b , R 5 , R 6a1 , R 6b1 , R 6a2 , R 6b2 , R 7a , and R 7b Each of is independently H, halogen, OH, CN, substituted or unsubstituted amino, or methyl.

[0123] In one embodiment, ring A is [ka] and ring B is [ka] where R a and R b each independently represents a substituted or unsubstituted C 1~4 Alkyl, substituted or unsubstituted C 3~5 cycloalkyl or R a and R b together with the N to which they are attached form a substituted or unsubstituted heterocycle containing N, O, or S; R c is H, halogen, CN, substituted or unsubstituted C 1~4 Alkyl, substituted or unsubstituted C 3~5 Cycloalkyl, substituted or unsubstituted C 1~4 is alkoxyl, and R 3a , R 3b , R 5 , R 6a1 , R 6b1 , R 6a2 , R 6b2 , R 7a , and R 7b Each of is independently H, halogen, OH, CN, substituted or unsubstituted amino, or methyl.

[0124] In one embodiment, ring B is [ka] is.

[0125] Group 1.2.1.4.1: In one embodiment, ring B is [ka] In one embodiment, ring B is [ka] is.

[0126] In one embodiment, R 3a , R 3b , R 5 , R 6a1 , R 6b1 , R 6a2 , R 6b2 , R 7a , and R 7b each independently represents H, halogen, OH, CN, unsubstituted or substituted amino, or unsubstituted or substituted C 1~4 In one embodiment, R 3a , R 3b , R 5 , R 6a1 , R 6b1 , R 6a2 , R 6b2 , R 7a , and R 7b Each of R is independently H, halogen, OH, CN, methylamino, methyl or ethyl optionally substituted with OH, CN, amino. 3a , R 3b , R 5 , R 6a1 , R 6b1 , R 6a2 , R 6b2 , R 7a , and R 7b is independently H, halogen, OH, CN, methylamino, or methyl.

[0127] In one embodiment, the compound is [ka] is.

[0128] Group 1.2.2: In one embodiment, X is CH.

[0129] In one embodiment, R 3a , R 3b , R 5 , R 6a1 , R 6b1 , R6a2 , R 6b2 , R 7a , and R 7b Each of is independently H, halogen, OH, CN, substituted or unsubstituted amino, or methyl.

[0130] In one embodiment, ring A is [ka] and ring B is [ka] where R a and R b each independently represents a substituted or unsubstituted C 1~4 Alkyl, substituted or unsubstituted C 3~5 cycloalkyl or R a and R b together with the N to which they are attached form a substituted or unsubstituted heterocycle containing N, O, or S; R c is H, halogen, CN, substituted or unsubstituted C 1~4 Alkyl, substituted or unsubstituted C 3~5 Cycloalkyl, substituted or unsubstituted C 1~4 is alkoxyl, and R 3a , R 3b , R 5 , R 6a1 , R 6b1 , R 6a2 , R 6b2 , R 7a , and R 7b Each of is independently H, halogen, OH, CN, substituted or unsubstituted amino, or methyl.

[0131] In one embodiment, ring A is [ka] and ring B is [ka] is.

[0132] Group 1.2.2.4: In one embodiment, ring A is [ka] is.

[0133] In one embodiment, ring B is [ka] is.

[0134] Group 1.2.2.4.1: In one embodiment, ring B is [ka] In one embodiment, ring B is [ka] is.

[0135] In one embodiment, R 3a , R 3b , R 5 , R 6a1 , R 6b1 , R 6a2 , R 6b2 , R 7a , and R 7b each independently represents H, halogen, OH, CN, unsubstituted or substituted amino, or unsubstituted or substituted C 1~4 In one embodiment, R 3a , R 3b , R 5 , R 6a1 , R 6b1 , R 6a2 , R 6b2 , R 7a , and R 7b Each of R is independently H, halogen, OH, CN, methylamino, methyl or ethyl optionally substituted with OH, CN, amino. 3a , R 3b , R 5 , R6a1 , R 6b1 , R 6a2 , R 6b2 , R 7a , and R 7b is independently H, halogen, OH, CN, methylamino, or methyl.

[0136] In one embodiment, the compound is [ka] is.

[0137] In one embodiment, R 6a2 and R 7a together with the atom to which they are attached form an unsubstituted or substituted cycloalkyl, or an unsubstituted or substituted heterocyclyl. 6a2 and R 7a are unsubstituted or substituted C, along with the atoms to which they are attached. 3~6 In one embodiment, the compound is: [ka] is.

[0138] Group 1.2.2.6: In one embodiment, ring A is [ka] is.

[0139] In one embodiment, R 3a , R 3b , R 5 , R 6a1 , R 6b1 , R 6a2 , R 6b2 , R 7a , and R 7b Each of is independently H, halogen, OH, CN, substituted or unsubstituted amino, or methyl.

[0140] In one embodiment, ring A is [ka] and ring B is [ka] where R a and R b each independently represents a substituted or unsubstituted C 1~4 Alkyl, substituted or unsubstituted C 3~5 cycloalkyl or R a and R b together with the N to which they are attached form a substituted or unsubstituted heterocycle containing N, O, or S; R c is H, halogen, CN, substituted or unsubstituted C 1~4 Alkyl, substituted or unsubstituted C 3~5 Cycloalkyl, substituted or unsubstituted C 1~4 is alkoxyl, and R 3a , R 3b , R 5 , R 6a1 , R 6b1 , R 6a2 , R 6b2 , R 7a , and R 7b Each of is independently H, halogen, OH, CN, substituted or unsubstituted amino, or methyl.

[0141] In one embodiment, ring B is [ka] is.

[0142] Group 1.2.2.6.1: In one embodiment, ring B is [ka] In one embodiment, ring B is [ka] is.

[0143] In one embodiment, R 3a , R 3b , R 5 , R 6a1 , R 6b1 , R 6a2 , R 6b2 , R 7a , and R 7b each independently represents H, halogen, OH, CN, unsubstituted or substituted amino, or unsubstituted or substituted C 1~4 In one embodiment, R 3a , R 3b , R 5 , R 6a1 , R 6b1 , R 6a2 , R 6b2 , R 7a , and R 7b Each of R is independently H, halogen, OH, CN, methylamino, methyl or ethyl optionally substituted with OH, CN, amino. 3a , R 3b , R 5 , R 6a1 , R 6b1 , R 6a2 , R 6b2 , R 7a , and R 7b is independently H, halogen, OH, CN, methylamino, or methyl.

[0144] In one embodiment, the compound is [ka] is.

[0145] Group 1.2.3: In one embodiment, X is CF.

[0146] In one embodiment, ring A is [ka] and ring B is [ka] is.

[0147] Group 1.2.3.4: In one embodiment, ring A is [ka] is.

[0148] In one embodiment, ring B is [ka] is.

[0149] Group 1.2.3.4.3: In one embodiment, ring B is [ka] where R a and R b each independently represents a substituted or unsubstituted C 1~4 Alkyl, substituted or unsubstituted C 3~5 cycloalkyl or R a and R b together with the N to which they are attached form a substituted or unsubstituted heterocycle containing N, O, or S; R c is H, halogen, CN, substituted or unsubstituted C 1~4 Alkyl, substituted or unsubstituted C 3~5 Cycloalkyl, substituted or unsubstituted C 1~4 In one embodiment, Ring B is [ka] is.

[0150] In one embodiment, R 3a , R 3b , R 5 , R 6a1 , R 6b1 , R 6a2 , R 6b2 , R7a , and R 7b each independently represents H, halogen, OH, CN, unsubstituted or substituted amino, or unsubstituted or substituted C 1~4 In one embodiment, R 3a , R 3b , R 5 , R 6a1 , R 6b1 , R 6a2 , R 6b2 , R 7a , and R 7b Each of R is independently H, halogen, OH, CN, methylamino, methyl or ethyl optionally substituted with OH, CN, amino. 3a , R 3b , R 5 , R 6a1 , R 6b1 , R 6a2 , R 6b2 , R 7a , and R 7b is independently H, halogen, OH, CN, methylamino, or methyl.

[0151] In one embodiment, the compound is [ka] is.

[0152] Group 1.2.4: In one embodiment, X is C—Cl.

[0153] In one embodiment, ring A is [ka] and ring B is [ka] is.

[0154] Group 1.2.4.2: In one embodiment, ring A is [ka] is.

[0155] In one embodiment, ring B is [ka] is.

[0156] Group 1.2.4.2.1: In one embodiment, ring B is [ka] In one embodiment, ring B is [ka] is.

[0157] In one embodiment, R 3a , R 3b , R 5 , R 6a1 , R 6b1 , R 6a2 , R 6b2 , R 7a , and R 7b each independently represents H, halogen, OH, CN, unsubstituted or substituted amino, or unsubstituted or substituted C 1~4 In one embodiment, R 3a , R 3b , R 5 , R 6a1 , R 6b1 , R 6a2 , R 6b2 , R 7a , and R 7b Each of R is independently H, halogen, OH, CN, methylamino, methyl or ethyl optionally substituted with OH, CN, amino. 3a , R 3b , R 5 , R 6a1 , R 6b1 , R 6a2 , R 6b2 , R 7a , and R 7bis independently H, halogen, OH, CN, methylamino, or methyl.

[0158] In one embodiment, the compound is [ka] is.

[0159] Group 1.2.4.6: In one embodiment, ring A is [ka] is.

[0160] In one embodiment, ring B is [ka] is.

[0161] Group 1.2.4.6.1: In one embodiment, ring B is [ka] In one embodiment, ring B is [ka] is.

[0162] In one embodiment, R 3a , R 3b , R 5 , R 6a1 , R 6b1 , R 6a2 , R 6b2 , R 7a , and R 7b each independently represents H, halogen, OH, CN, unsubstituted or substituted amino, or unsubstituted or substituted C 1~4 In one embodiment, R 3a , R 3b , R 5 , R 6a1 , R 6b1 , R 6a2 , R6b2 , R 7a , and R 7b Each of R is independently H, halogen, OH, CN, methylamino, methyl or ethyl optionally substituted with OH, CN, amino. 3a , R 3b , R 5 , R 6a1 , R 6b1 , R 6a2 , R 6b2 , R 7a , and R 7b is independently H, halogen, OH, CN, methylamino, or methyl.

[0163] In one embodiment, the compound is [ka] is.

[0164] Group 1.2.5: In one embodiment, X is C-CF3.

[0165] Group 1.2.5.6: In one embodiment, ring A is [ka] is.

[0166] In one embodiment, ring B is [ka] is.

[0167] Group 1.2.5.6.1: In one embodiment, ring B is [ka] In one embodiment, ring B is [ka] is.

[0168] In one embodiment, R 3a , R 3b , R 5 , R 6a1 , R 6b1 , R 6a2 , R 6b2 , R 7a , and R 7b each independently represents H, halogen, OH, CN, unsubstituted or substituted amino, or unsubstituted or substituted C 1~4 In one embodiment, R 3a , R 3b , R 5 , R 6a1 , R 6b1 , R 6a2 , R 6b2 , R 7a , and R 7b Each of R is independently H, halogen, OH, CN, methylamino, methyl or ethyl optionally substituted with OH, CN, amino. 3a , R 3b , R 5 , R 6a1 , R 6b1 , R 6a2 , R 6b2 , R 7a , and R 7b is independently H, halogen, OH, CN, methylamino, or methyl.

[0169] In one embodiment, the compound is [ka] is.

[0170] Group 1.3: In one embodiment, u is 3. In one embodiment, the compound has formula (IV): [ka] It is a compound having the formula:

[0171] In one embodiment, R 3a , R 3b , R 5 , R 6a1 , R6b1 , R 6a2 , R 6b2 , R 6a3 , R 6b3 , R 7a , and R 7b Each of is independently H, halogen, OH, CN, substituted or unsubstituted amino, or methyl.

[0172] In one embodiment, ring B is [ka] where R a and R b each independently represents a substituted or unsubstituted C 1~4 Alkyl, substituted or unsubstituted C 3~5 cycloalkyl or R a and R b together with the N to which they are attached form a substituted or unsubstituted heterocycle containing N, O, or S; R c is H, halogen, CN, substituted or unsubstituted C 1~4 Alkyl, substituted or unsubstituted C 3~5 Cycloalkyl, substituted or unsubstituted C 1~4 is alkoxyl, and R 3a , R 3b , R 5 , R 6a1 , R 6b1 , R 6a2 , R 6b2 , R 6a3 , R 6b3 , R 7a , and R 7b Each of is independently H, halogen, OH, CN, substituted or unsubstituted amino, or methyl.

[0173] Group 1.3.1: In one embodiment, X is N.

[0174] In one embodiment, ring A is [ka] and ring B is [ka] is.

[0175] Group 1.3.1.1: In one embodiment, ring A is [ka] is.

[0176] In one embodiment, ring B is [ka] is.

[0177] Group 1.3.1.1.1: In one embodiment, ring B is [ka] In one embodiment, ring B is [ka] is.

[0178] In one embodiment, R 3a , R 3b , R 5 , R 6a1 , R 6b1 , R 6a2 , R 6b2 , R 6a3 , R 6b3 , R 7a , and R 7b each independently represents H, halogen, OH, CN, unsubstituted or substituted amino, or unsubstituted or substituted C 1~4 In one embodiment, R 3a , R 3b , R 5 , R 6a1 , R 6b1 , R 6a2 , R 6b2 , R 6a3 , R 6b3 , R 7a , and R7b Each of R is independently H, halogen, OH, CN, methylamino, methyl or ethyl optionally substituted with OH, CN, amino. 3a , R 3b , R 5 , R 6a1 , R 6b1 , R 6a2 , R 6b2 , R 6a3 , R 6b3 , R 7a , and R 7b is independently H, halogen, OH, CN, methylamino, hydroxylmethyl, or methyl.

[0179] In one embodiment, the compound is [ka] [ka] is.

[0180] Group 1.3.1.4: In one embodiment, ring A is [ka] is.

[0181] In one embodiment, ring B is [ka] is.

[0182] Group 1.3.1.4.1: In one embodiment, ring B is [ka] In one embodiment, ring B is [ka] is.

[0183] In one embodiment, R 3a , R 3b , R 5 , R 6a1 , R 6b1 , R 6a2 , R 6b2 , R 6a3 , R 6b3 , R 7a , and R 7b each independently represents H, halogen, OH, CN, unsubstituted or substituted amino, or unsubstituted or substituted C 1~4 In one embodiment, R 3a , R 3b , R 5 , R 6a1 , R 6b1 , R 6a2 , R 6b2 , R 6a3 , R 6b3 , R 7a , and R 7b Each of R is independently H, halogen, OH, CN, methylamino, methyl or ethyl optionally substituted with OH, CN, amino. 3a , R 3b , R 5 , R 6a1 , R 6b1 , R 6a2 , R 6b2 , R 6a3 , R 6b3 , R 7a , and R 7b is independently H, halogen, OH, CN, methylamino, or methyl.

[0184] In one embodiment, the compound is [ka] is.

[0185] Group 1.3.4: In one embodiment, X is C—Cl.

[0186] In one embodiment, ring A is [ka] and ring B is [ka] is.

[0187] Group 1.3.4.6: In one embodiment, ring A is [ka] is.

[0188] In one embodiment, ring B is [ka] is.

[0189] Group 1.3.4.6.1: In one embodiment, ring B is [ka] In one embodiment, ring B is [ka] is.

[0190] In one embodiment, R 3a , R 3b , R 5 , R 6a1 , R 6b1 , R 6a2 , R 6b2 , R 6a3 , R 6b3 , R 7a , and R 7b each independently represents H, halogen, OH, CN, unsubstituted or substituted amino, or unsubstituted or substituted C 1~4 In one embodiment, R 3a , R 3b , R 5 , R 6a1 , R 6b1 , R 6a2 , R 6b2 , R 6a3 , R 6b3 , R7a , and R 7b Each of R is independently H, halogen, OH, CN, methylamino, methyl or ethyl optionally substituted with OH, CN, amino. 3a , R 3b , R 5 , R 6a1 , R 6b1 , R 6a2 , R 6b2 , R 6a3 , R 6b3 , R 7a , and R 7b is independently H, halogen, OH, CN, methylamino, or methyl.

[0191] In one embodiment, the compound is [ka] is.

[0192] Group 1.3.4.7: In one embodiment, ring A is [ka] is.

[0193] Group 1.3.4.7.3: In one embodiment, ring B is [ka] is.

[0194] In one embodiment, R 3a , R 3b , R 5 , R 6a1 , R 6b1 , R 6a2 , R 6b2 , R 6a3 , R 6b3 , R 7a , and R 7b each independently represents H, halogen, OH, CN, unsubstituted or substituted amino, or unsubstituted or substituted C 1~4 In one embodiment, R3a , R 3b , R 5 , R 6a1 , R 6b1 , R 6a2 , R 6b2 , R 6a3 , R 6b3 , R 7a , and R 7b Each of R is independently H, halogen, OH, CN, methylamino, methyl or ethyl optionally substituted with OH, CN, amino. 3a , R 3b , R 5 , R 6a1 , R 6b1 , R 6a2 , R 6b2 , R 6a3 , R 6b3 , R 7a , and R 7b is independently H, halogen, OH, CN, methylamino, or methyl.

[0195] In one embodiment, the compound is [ka] is.

[0196] Group 1.3.4.7.6: In one embodiment, ring B is [ka] is.

[0197] In one embodiment, R 3a , R 3b , R 5 , R 6a1 , R 6b1 , R 6a2 , R 6b2 , R 6a3 , R 6b3 , R 7a , and R 7b each independently represents H, halogen, OH, CN, unsubstituted or substituted amino, or unsubstituted or substituted C 1~4 In one embodiment, R3a , R 3b , R 5 , R 6a1 , R 6b1 , R 6a2 , R 6b2 , R 6a3 , R 6b3 , R 7a , and R 7b Each of R is independently H, halogen, OH, CN, methylamino, methyl or ethyl optionally substituted with OH, CN, amino. 3a , R 3b , R 5 , R 6a1 , R 6b1 , R 6a2 , R 6b2 , R 6a3 , R 6b3 , R 7a , and R 7b is independently H, halogen, OH, CN, methylamino, or methyl.

[0198] In one embodiment, the compound is [ka] is.

[0199] Group 1.4: In one embodiment, u is 4. In one embodiment, the compound has the formula (V): [ka] It is a compound having the formula:

[0200] In one embodiment, R 3a , R 3b , R 5 , R 6a1 , R 6b1 , R 6a2 , R 6b2 , R 6a3 , R 6b3 , R 6a4 , R 6b4 , R 7a , and R 7bEach of is independently H, halogen, OH, CN, substituted or unsubstituted amino, or methyl.

[0201] In one embodiment, ring B is [ka] where R a and R b each independently represents a substituted or unsubstituted C 1~4 Alkyl, substituted or unsubstituted C 3~5 cycloalkyl or R a and R b together with the N to which they are attached form a substituted or unsubstituted heterocycle containing N, O, or S; R c is H, halogen, CN, substituted or unsubstituted C 1~4 Alkyl, substituted or unsubstituted C 3~5 Cycloalkyl, substituted or unsubstituted C 1~4 is alkoxyl, and R 3a , R 3b , R 5 , R 6a1 , R 6b1 , R 6a2 , R 6b2 , R 6a3 , R 6b3 , R 6a4 , R 6b4 , R 7a , and R 7b Each of is independently H, halogen, OH, CN, substituted or unsubstituted amino, or methyl.

[0202] Group 1.4.1: In one embodiment, X is N.

[0203] In one embodiment, ring A is [ka] and ring B is [ka] is.

[0204] Group 1.4.1.1: In one embodiment, ring A is [ka] is.

[0205] In one embodiment, ring B is [ka] is.

[0206] Group 1.4.1.1.1: In one embodiment, ring B is [ka] In one embodiment, ring B is [ka] is.

[0207] In one embodiment, R 3a , R 3b , R 5 , R 6a1 , R 6b1 , R 6a2 , R 6b2 , R 6a3 , R 6b3 , R 6a4 , R 6b4 , R 7a , and R 7b each independently represents H, halogen, OH, CN, unsubstituted or substituted amino, or unsubstituted or substituted C 1~4 In one embodiment, R 3a , R 3b , R 5 , R 6a1 , R 6b1 , R 6a2 , R 6b2 , R 6a3 , R 6b3 , R 6a4 , R 6b4 , R 7a , and R 7bEach of R is independently H, halogen, OH, CN, methylamino, methyl or ethyl optionally substituted with OH, CN, amino. 3a , R 3b , R 5 , R 6a1 , R 6b1 , R 6a2 , R 6b2 , R 6a3 , R 6b3 , R 6a4 , R 6b4 , R 7a , and R 7b is independently H, halogen, OH, CN, methylamino, or methyl.

[0208] In one embodiment, the compound is [ka] is.

[0209] Aspect 8:

[0210] Group 2: In one embodiment, t is 1.

[0211] Group 2.1: In one embodiment, u is 1. In one embodiment, the compound has formula (VI): [ka] It is a compound having the formula:

[0212] In one embodiment, R 3a , R 3b , R 4a , R 4b , R 5 , R 6a1 , R 6b1 , R 7a , and R 7b Each of is independently H, halogen, OH, CN, substituted or unsubstituted amino, or methyl.

[0213] In one embodiment, ring B is [ka] where R a and R b each independently represents a substituted or unsubstituted C 1~4 Alkyl, substituted or unsubstituted C 3~5 cycloalkyl or R a and R b together with the N to which they are attached form a substituted or unsubstituted heterocycle containing N, O, or S; R c is H, halogen, CN, substituted or unsubstituted C 1~4 Alkyl, substituted or unsubstituted C 3~5 Cycloalkyl, substituted or unsubstituted C 1~4 is alkoxyl, R 3a , R 3b , R 4a , R 4b , R 5 , R 6a1 , R 6b1 , R 7a , and R 7b Each of is independently H, halogen, OH, CN, substituted or unsubstituted amino, or methyl.

[0214] Group 2.1.1: In one embodiment, X is N.

[0215] In one embodiment, ring B is [ka] where R a and R b each independently represents a substituted or unsubstituted C 1~4 Alkyl, substituted or unsubstituted C 3~5 cycloalkyl or R a and R b together with the N to which they are attached form a substituted or unsubstituted heterocycle containing N, O, or S; R c is H, halogen, CN, substituted or unsubstituted C 1~4Alkyl, substituted or unsubstituted C 3~5 Cycloalkyl, substituted or unsubstituted C 1~4 It is alkoxyl.

[0216] In one embodiment, ring A is [ka] and ring B is [ka] is.

[0217] Group 2.1.1.1: In one embodiment, ring A is [ka] is.

[0218] In one embodiment, ring B is [ka] is.

[0219] Group 2.1.1.1.1: In one embodiment, ring B is [ka] In one embodiment, ring B is [ka] is.

[0220] In one embodiment, R 3a , R 3b , R 4a , R 4b , R 5 , R 6a1 , R 6b1 , R 7a , and R 7b each independently represents H, halogen, OH, CN, unsubstituted or substituted amino, or unsubstituted or substituted C 1~4In one embodiment, R 3a , R 3b , R 4a , R 4b , R 5 , R 6a1 , R 6b1 , R 7a , and R 7b Each of R is independently H, halogen, OH, CN, methylamino, methyl or ethyl optionally substituted with OH, CN, amino. 3a , R 3b , R 4a , R 4b , R 5 , R 6a1 , R 6b1 , R 7a , and R 7b is independently H, halogen, OH, CN, methylamino, or methyl.

[0221] In one embodiment, the compound is [ka] is.

[0222] Group 2.2: In one embodiment, u is 2. In one embodiment, the compound has formula (VII): [ka] It is a compound having the formula:

[0223] In one embodiment, R 3a , R 3b , R 4a , R 4b , R 5 , R 6a1 , R 6b1 , R 6a2 , R 6b2 , R 7a , and R 7b Each of is independently H, halogen, OH, CN, substituted or unsubstituted amino, or methyl.

[0224] In one embodiment, ring B is [ka] where R a and R b each independently represents a substituted or unsubstituted C 1~4 Alkyl, substituted or unsubstituted C 3~5 cycloalkyl or R a and R b together with the N to which they are attached form a substituted or unsubstituted heterocycle containing N, O, or S; R c is H, halogen, CN, substituted or unsubstituted C 1~4 Alkyl, substituted or unsubstituted C 3~5 Cycloalkyl, substituted or unsubstituted C 1~4 is alkoxyl, R 3a , R 3b , R 4a , R 4b , R 5 , R 6a1 , R 6b1 , R 6a2 , R 6b2 , R 7a , and R 7b Each of is independently H, halogen, OH, CN, substituted or unsubstituted amino, or methyl.

[0225] Group 2.2.1: In one embodiment, X is N.

[0226] In one embodiment, ring B is [ka] where R a and R b each independently represents a substituted or unsubstituted C 1~4 Alkyl, substituted or unsubstituted C 3~5 cycloalkyl or R a and R b together with the N to which they are attached form a substituted or unsubstituted heterocycle containing N, O, or S; R c is H, halogen, CN, substituted or unsubstituted C 1~4 Alkyl, substituted or unsubstituted C 3~5 Cycloalkyl, substituted or unsubstituted C 1~4 It is alkoxyl.

[0227] In one embodiment, ring A is [ka] and ring B is [ka] is.

[0228] Group 2.2.1.1: In one embodiment, ring A is [ka] is.

[0229] In one embodiment, ring B is [ka] is.

[0230] Group 2.2.1.1.1: In one embodiment, ring B is [ka] In one embodiment, ring B is [ka] is.

[0231] In one embodiment, R 3a , R 3b , R 4a , R 4b , R 5 , R 6a1 , R 6b1 , R 6a2 , R 6b2 , R 7a, and R 7b each independently represents H, halogen, OH, CN, unsubstituted or substituted amino, or unsubstituted or substituted C 1~4 In one embodiment, R 3a , R 3b , R 4a , R 4b , R 5 , R 6a1 , R 6b1 , R 6a2 , R 6b2 , R 7a , and R 7b Each of R is independently H, halogen, OH, CN, methylamino, methyl or ethyl optionally substituted with OH, CN, amino. 3a , R 3b , R 4a , R 4b , R 5 , R 6a1 , R 6b1 , R 6a2 , R 6b2 , R 7a , and R 7b is independently H, halogen, OH, CN, methylamino, hydroxylmethyl, or methyl.

[0232] In one embodiment, the compound is [ka] is.

[0233] Group 2.2.1.1.3: In one embodiment, ring B is [ka] is.

[0234] In one embodiment, R 3a , R 3b , R 4a , R 4b , R 5 , R 6a1 , R 6b1 , R 6a2 , R 6b2 , R7a , and R 7b each independently represents H, halogen, OH, CN, unsubstituted or substituted amino, or unsubstituted or substituted C 1~4 In one embodiment, R 3a , R 3b , R 4a , R 4b , R 5 , R 6a1 , R 6b1 , R 6a2 , R 6b2 , R 7a , and R 7b Each of R is independently H, halogen, OH, CN, methylamino, methyl or ethyl optionally substituted with OH, CN, amino. 3a , R 3b , R 4a , R 4b , R 5 , R 6a1 , R 6b1 , R 6a2 , R 6b2 , R 7a , and R 7b is independently H, halogen, OH, CN, methylamino, or methyl.

[0235] In one embodiment, the compound is [ka] is.

[0236] Group 2.2.1.2: In one embodiment, ring A is [ka] is.

[0237] In one embodiment, ring B is [ka] is.

[0238] Group 2.2.1.2.1: In one embodiment, ring B is [ka] In one embodiment, ring B is [ka] is.

[0239] In one embodiment, R 3a , R 3b , R 4a , R 4b , R 5 , R 6a1 , R 6b1 , R 6a2 , R 6b2 , R 7a , and R 7b each independently represents H, halogen, OH, CN, unsubstituted or substituted amino, or unsubstituted or substituted C 1~4 In one embodiment, R 3a , R 3b , R 4a , R 4b , R 5 , R 6a1 , R 6b1 , R 6a2 , R 6b2 , R 7a , and R 7b Each of R is independently H, halogen, OH, CN, methylamino, methyl or ethyl optionally substituted with OH, CN, amino. 3a , R 3b , R 4a , R 4b , R 5 , R 6a1 , R 6b1 , R 6a2 , R 6b2 , R 7a , and R 7b is independently H, halogen, OH, CN, methylamino, or methyl.

[0240] In one embodiment, the compound is [ka] is.

[0241] Group 2.2.2: In one embodiment, X is CH.

[0242] In one embodiment, ring B is [ka] where R a and R b each independently represents a substituted or unsubstituted C 1~4 Alkyl, substituted or unsubstituted C 3~5 cycloalkyl or R a and R b together with the N to which they are attached form a substituted or unsubstituted heterocycle containing N, O, or S; R c is H, halogen, CN, substituted or unsubstituted C 1~4 Alkyl, substituted or unsubstituted C 3~5 Cycloalkyl, substituted or unsubstituted C 1~4 It is alkoxyl.

[0243] In one embodiment, ring A is [ka] and ring B is [ka] is.

[0244] Group 2.2.2.4: In one embodiment, ring A is [ka] is.

[0245] In one embodiment, ring B is [ka] is.

[0246] Group 2.2.2.4.1: In one embodiment, ring B is [ka] In one embodiment, ring B is [ka] is.

[0247] In one embodiment, R 3a , R 3b , R 4a , R 4b , R 5 , R 6a1 , R 6b1 , R 6a2 , R 6b2 , R 7a , and R 7b each independently represents H, halogen, OH, CN, unsubstituted or substituted amino, or unsubstituted or substituted C 1~4 In one embodiment, R 3a , R 3b , R 4a , R 4b , R 5 , R 6a1 , R 6b1 , R 6a2 , R 6b2 , R 7a , and R 7b Each of R is independently H, halogen, OH, CN, methylamino, methyl or ethyl optionally substituted with OH, CN, amino. 3a , R 3b , R 4a , R 4b , R 5 , R 6a1 , R 6b1 , R 6a2 , R 6b2 , R 7a , and R 7b is independently H, halogen, OH, CN, methylamino, or methyl.

[0248] In one embodiment, the compound is [ka] is.

[0249] Group 2.2.2.6: In one embodiment, ring A is [ka] is.

[0250] In one embodiment, ring B is [ka] is.

[0251] Group 2.2.2.6.1: In one embodiment, ring B is [ka] In one embodiment, ring B is [ka] is.

[0252] In one embodiment, R 3a , R 3b , R 4a , R 4b , R 5 , R 6a1 , R 6b1 , R 6a2 , R 6b2 , R 7a , and R 7b each independently represents H, halogen, OH, CN, unsubstituted or substituted amino, or unsubstituted or substituted C 1~4 In one embodiment, R 3a , R 3b , R 4a , R 4b , R 5 , R 6a1 , R 6b1 , R 6a2 , R 6b2 , R 7a , and R7b Each of R is independently H, halogen, OH, CN, methylamino, methyl or ethyl optionally substituted with OH, CN, amino. 3a , R 3b , R 4a , R 4b , R 5 , R 6a1 , R 6b1 , R 6a2 , R 6b2 , R 7a , and R 7b is independently H, halogen, OH, CN, methylamino, or methyl.

[0253] In one embodiment, the compound is [ka] is.

[0254] Group 2.2.4: In one embodiment, X is C—Cl.

[0255] In one embodiment, ring B is [ka] where R a and R b each independently represents a substituted or unsubstituted C 1~4 Alkyl, substituted or unsubstituted C 3~5 cycloalkyl or R a and R b together with the N to which they are attached form a substituted or unsubstituted heterocycle containing N, O, or S; R c is H, halogen, CN, substituted or unsubstituted C 1~4 Alkyl, substituted or unsubstituted C 3~5 Cycloalkyl, substituted or unsubstituted C 1~4 It is alkoxyl.

[0256] Group 2.2.4.6: In one embodiment, ring A is [ka] is.

[0257] In one embodiment, ring B is [ka] is.

[0258] Group 2.2.4.6.1: In one embodiment, ring B is [ka] In one embodiment, ring B is [ka] is.

[0259] In one embodiment, R 3a , R 3b , R 4a , R 4b , R 5 , R 6a1 , R 6b1 , R 6a2 , R 6b2 , R 7a , and R 7b each independently represents H, halogen, OH, CN, unsubstituted or substituted amino, or unsubstituted or substituted C 1~4 In one embodiment, R 3a , R 3b , R 4a , R 4b , R 5 , R 6a1 , R 6b1 , R 6a2 , R 6b2 , R 7a , and R 7b Each of R is independently H, halogen, OH, CN, methylamino, methyl or ethyl optionally substituted with OH, CN, amino. 3a , R 3b , R 4a , R 4b , R 5, R 6a1 , R 6b1 , R 6a2 , R 6b2 , R 7a , and R 7b is independently H, halogen, OH, CN, methylamino, or methyl.

[0260] In one embodiment, the compound is [ka] is.

[0261] Group 2.2.4.6.3: In one embodiment, ring B is [ka] is.

[0262] In one embodiment, R 3a , R 3b , R 4a , R 4b , R 5 , R 6a1 , R 6b1 , R 6a2 , R 6b2 , R 7a , and R 7b each independently represents H, halogen, OH, CN, unsubstituted or substituted amino, or unsubstituted or substituted C 1~4 In one embodiment, R 3a , R 3b , R 4a , R 4b , R 5 , R 6a1 , R 6b1 , R 6a2 , R 6b2 , R 7a , and R 7b Each of R is independently H, halogen, OH, CN, methylamino, methyl or ethyl optionally substituted with OH, CN, amino. 3a , R 3b , R 4a , R 4b , R 5 , R6a1 , R 6b1 , R 6a2 , R 6b2 , R 7a , and R 7b is independently H, halogen, OH, CN, methylamino, or methyl.

[0263] In one embodiment, the compound is [ka] is.

[0264] Group 2.2.5: In one embodiment, X is C-CF3.

[0265] In one embodiment, ring B is [ka] where R a and R b each independently represents a substituted or unsubstituted C 1~4 Alkyl, substituted or unsubstituted C 3~5 cycloalkyl or R a and R b together with the N to which they are attached form a substituted or unsubstituted heterocycle containing N, O, or S; R c is H, halogen, CN, substituted or unsubstituted C 1~4 Alkyl, substituted or unsubstituted C 3~5 Cycloalkyl, substituted or unsubstituted C 1~4 It is alkoxyl.

[0266] Group 2.2.5.6: In one embodiment, ring A is [ka] is.

[0267] In one embodiment, ring B is [ka] is.

[0268] Group 2.2.5.6.1: In one embodiment, ring B is [ka] In one embodiment, ring B is [ka] is.

[0269] In one embodiment, R 3a , R 3b , R 4a , R 4b , R 5 , R 6a1 , R 6b1 , R 6a2 , R 6b2 , R 7a , and R 7b each independently represents H, halogen, OH, CN, unsubstituted or substituted amino, or unsubstituted or substituted C 1~4 In one embodiment, R 3a , R 3b , R 4a , R 4b , R 5 , R 6a1 , R 6b1 , R 6a2 , R 6b2 , R 7a , and R 7b Each of R is independently H, halogen, OH, CN, methylamino, methyl or ethyl optionally substituted with OH, CN, amino. 3a , R 3b , R 4a , R 4b , R 5 , R 6a1 , R 6b1 , R 6a2 , R 6b2 , R 7a , and R 7b is independently H, halogen, OH, CN, methylamino, or methyl.

[0270] In one embodiment, the compound is [ka] is.

[0271] Group 2.3: In one embodiment, u is 3. In one embodiment, the compound has formula (VIII): [ka] It is a compound having the formula:

[0272] In one embodiment, R 3a , R 3b , R 4a , R 4b , R 5 , R 6a1 , R 6b1 , R 6a2 , R 6b2 , R 6a3 , R 6b3 , R 7a , and R 7b Each of is independently H, halogen, OH, CN, substituted or unsubstituted amino, or methyl.

[0273] In one embodiment, ring B is [ka] where R a and R b each independently represents a substituted or unsubstituted C 1~4 Alkyl, substituted or unsubstituted C 3~5 cycloalkyl or R a and R b together with the N to which they are attached form a substituted or unsubstituted heterocycle containing N, O, or S; R c is H, halogen, CN, substituted or unsubstituted C 1~4 Alkyl, substituted or unsubstituted C 3~5 Cycloalkyl, substituted or unsubstituted C 1~4is alkoxyl, R 3a , R 3b , R 4a , R 4b , R 5 , R 6a1 , R 6b1 , R 6a2 , R 6b2 , R 6a3 , R 6b3 , R 7a , and R 7b Each of is independently H, halogen, OH, CN, substituted or unsubstituted amino, or methyl.

[0274] Group 2.3.1: In one embodiment, X is N.

[0275] In one embodiment, ring A is [ka] and ring B is [ka] is.

[0276] Group 2.3.1.1: In one embodiment, ring A is [ka] is.

[0277] In one embodiment, ring B is [ka] is.

[0278] Group 2.3.1.1.1: In one embodiment, ring B is [ka] In one embodiment, ring B is [ka] is.

[0279] In one embodiment, R 3a , R 3b , R 4a , R 4b , R 5 , R 6a 1. R 6b1 , R 6a2 , R 6b2 , R 6a3 , R 6b3 , R 7a , and R 7b each independently represents H, halogen, OH, CN, unsubstituted or substituted amino, or unsubstituted or substituted C 1~4 In one embodiment, R 3a , R 3b , R 4a , R 4b , R 5 , R 6a1 , R 6b1 , R 6a2 , R 6b2 , R 6a3 , R 6b3 , R 7a , and R 7b Each of R is independently H, halogen, OH, CN, methylamino, methyl or ethyl optionally substituted with OH, CN, amino. 3a , R 3b , R 4a , R 4b , R 5 , R 6a1 , R 6b1 , R 6a2 , R 6b2 , R 6a3 , R 6b3 , R 7a , and R 7b is independently H, halogen, OH, CN, methylamino, or methyl.

[0280] In one embodiment, the compound is [ka] is.

[0281] Group 2.3.2: In one embodiment, X is CH.

[0282] In one embodiment, ring B is [ka] where R a and R b each independently represents a substituted or unsubstituted C 1~4 Alkyl, substituted or unsubstituted C 3~5 cycloalkyl or R a and R b together with the N to which they are attached form a substituted or unsubstituted heterocycle containing N, O, or S; R c is H, halogen, CN, substituted or unsubstituted C 1~4 Alkyl, substituted or unsubstituted C 3~5 Cycloalkyl, substituted or unsubstituted C 1~4 It is alkoxyl.

[0283] In one embodiment, ring A is [ka] and ring B is [ka] is.

[0284] Group 2.3.2.4: In one embodiment, ring A is [ka] is.

[0285] In one embodiment, ring B is [ka] is.

[0286] Group 2.3.2.4.1: In one embodiment, ring B is [ka] In one embodiment, ring B is [ka] is.

[0287] In one embodiment, R 3a , R 3b , R 4a , R 4b , R 5 , R 6a1 , R 6b1 , R 6a2 , R 6b2 , R 7a , and R 7b each independently represents H, halogen, OH, CN, unsubstituted or substituted amino, or unsubstituted or substituted C 1~4 In one embodiment, R 3a , R 3b , R 4a , R 4b , R 5 , R 6a1 , R 6b1 , R 6a2 , R 6b2 , R 7a , and R 7b Each of R is independently H, halogen, OH, CN, methylamino, methyl or ethyl optionally substituted with OH, CN, amino. 3a , R 3b , R 4a , R 4b , R 5 , R 6a1 , R 6b1 , R 6a2 , R 6b2 , R 7a , and R 7b is independently H, halogen, OH, CN, methylamino, or methyl.

[0288] In one embodiment, the compound is [ka] is.

[0289] Group 2.4: In one embodiment, u is 4. In one embodiment, the compound has formula (IX): [ka] It is a compound having the formula:

[0290] In one embodiment, R 3a , R 3b , R 4a , R 4b , R 5 , R 6a1 , R 6b1 , R 6a2 , R 6b2 , R 6a3 , R 6b3 , R 6a4 , R 6b4 , R 7a , and R 7b Each of is independently H, halogen, OH, CN, substituted or unsubstituted amino, or methyl.

[0291] In one embodiment, ring B is [ka] where R a and R b each independently represents a substituted or unsubstituted C 1~4 Alkyl, substituted or unsubstituted C 3~5 cycloalkyl or R a and R b together with the N to which they are attached form a substituted or unsubstituted heterocycle containing N, O, or S; R c is H, halogen, CN, substituted or unsubstituted C 1~4 Alkyl, substituted or unsubstituted C 3~5 Cycloalkyl, substituted or unsubstituted C 1~4 is alkoxyl, R 3a , R 3b , R 4a , R4b , R 5 , R 6a1 , R 6b1 , R 6a2 , R 6b2 , R 6a3 , R 6b3 , R 6a4 , R 6b4 , R 7a , and R 7 Each b is independently H, halogen, OH, CN, substituted or unsubstituted amino, or methyl.

[0292] Group 2.4.1: In one embodiment, X is N.

[0293] In one embodiment, ring A is [ka] and ring B is [ka] is.

[0294] Group 2.4.1.1: In one embodiment, ring A is [ka] is.

[0295] In one embodiment, ring B is [ka] is.

[0296] Group 2.4.1.1.1: In one embodiment, ring B is [ka] In one embodiment, ring B is [ka] is.

[0297] In one embodiment, R 3a , R 3b , R 4a , R 4b , R 5 , R 6a 1. R 6b1 , R 6a2 , R 6b2 , R 6a3 , R 6b3 , R 6a4 , R 6b4 , R 7a , and R 7b each independently represents H, halogen, OH, CN, unsubstituted or substituted amino, or unsubstituted or substituted C 1~4 In one embodiment, R 3a , R 3b , R 4a , R 4b , R 5 , R 6a1 , R 6b1 , R 6a2 , R 6b2 , R 6a3 , R 6b3 , R 6a4 , R 6b4 , R 7a , and R 7b Each of R is independently H, halogen, OH, CN, methylamino, methyl or ethyl optionally substituted with OH, CN, amino. 3a , R 3b , R 4a , R 4b , R 5 , R 6a1 , R 6b1 , R 6a2 , R 6b2 , R 6a3 , R 6b3 , R 6a4 , R 6b4 , R 7a , and R 7b is independently H, halogen, OH, CN, methylamino, or methyl.

[0298] In one embodiment, the compound is [ka] is.

[0299] Aspect 9: In one embodiment, the compound is selected from Table 2 and Table 3.

[0300] Aspect 10: In some embodiments, compounds provided herein have the following formula: [ka] and pharmaceutically acceptable salts, tautomers, stereoisomers, enantiomers, atropisomers, isotopically substituted derivatives, and prodrugs thereof; During the ceremony, Ring C is unsubstituted or substituted C 3~6 cycloalkyl, or 3- to 6-membered unsubstituted or substituted heterocyclyl; each of u and v is independently an integer; The sum of u and v is 1, 2, 3, or 4.

[0301] Aspect 11: In some embodiments, compounds provided herein have the following formula: [ka] and pharmaceutically acceptable salts, tautomers, stereoisomers, enantiomers, atropisomers, isotopically substituted derivatives, and prodrugs thereof; During the ceremony, Y is CH2, O, NH, NR 9 , NC(=O)-R 10 , or O=S=O, R 9 is a substituted or unsubstituted C 1~4 Alkyl, or unsubstituted or substituted C 3~5 is cycloalkyl, R 10 is a substituted or unsubstituted C 1~4 Alkyl, unsubstituted or substituted C 1~4 Alkoxy, unsubstituted or substituted C 3~5cycloalkyl, or 3- to 5-membered unsubstituted or substituted heterocyclyl; R Y is H, halogen, amino, -CN, -OH, unsubstituted or substituted C 1~4 Alkyl, unsubstituted or substituted C 1~4 Alkoxy, or unsubstituted or substituted C 1~4 It is alkylamino.

[0302] In one embodiment, Y is O. In one embodiment, Y is N. In one embodiment, Y is CH.

[0303] Embodiment 12: Provided herein is a compound selected from the following table:

[0304] [Table 1-1] [Table 1-2] [Table 1-3]

[0305] Aspect 13: In one embodiment, provided herein is a pharmaceutical composition comprising an effective amount of a compound provided herein, or a pharmaceutically acceptable salt, tautomer, isotopic substitution, stereoisomer, enantiomer, atropisomer, or prodrug thereof, and a pharmaceutically acceptable carrier, excipient, or vehicle.

[0306] Aspect 14: In one embodiment, provided herein is a method for inhibiting the activity of a KRAS mutant protein in a cell, comprising contacting the cell with an effective amount of a compound provided herein, or a pharmaceutically acceptable salt, tautomer, isotopic substitution, stereoisomer, enantiomer, atropisomer, or prodrug thereof, wherein optionally the KRAS mutant protein is a KRAS G12D and / or G12V mutant protein. In one embodiment, provided herein is a method for inhibiting the activity of KRAS amplification in a cell, comprising contacting the cell with an effective amount of a compound provided herein, or a pharmaceutically acceptable salt, tautomer, isotopic substitution, stereoisomer, enantiomer, atropisomer, or prodrug thereof, wherein optionally the KRAS mutant protein is a KRAS G12D and / or G12V mutant protein.

[0307] Aspect 15: In one embodiment, provided herein is a method for the treatment or prevention of cancer, comprising administering to a subject in need thereof an effective amount of a compound provided herein, or a pharmaceutically acceptable salt, tautomer, isotopic substitute, stereoisomer, enantiomer, atropisomer, or prodrug thereof, wherein optionally the cancer is mediated by a KRAS mutation, preferably a KRAS G12D and / or G12V mutation. Provided herein is a method for the treatment or prevention of cancer, comprising administering to a subject in need thereof an effective amount of a compound provided herein.

[0308] Aspect 16: Provided herein is a method of modulating the activity of KRAS G12D and / or G12V, the method comprising contacting the cell with an effective amount of a compound provided herein, or a pharmaceutically acceptable salt, tautomer, isotopic substitute, stereoisomer, enantiomer, atropisomer, or prodrug thereof.

[0309] Embodiment 17: Provided herein is a kit for treating cancer, the kit comprising: (a) a pharmaceutical composition comprising a compound provided herein; and (b) instructions for administering an effective amount of the pharmaceutical composition comprising a KRAS G12D and / or G12V inhibitor provided herein to treat cancer in an individual.

[0310] Embodiments of the present invention may be more fully understood by reference to the detailed description and examples, which are intended to exemplify non-limiting embodiments.

[0311] Method for producing the compound The compounds can be prepared using conventional organic synthesis and commercially available starting materials. For example, but not limited to, compounds of formula (I) can be prepared as outlined in Schemes 1-2 shown below and in the Examples described herein. It should be noted that one skilled in the art would know how to modify the procedures described in the illustrative Schemes and Examples to arrive at the desired product. Common protecting groups can be used to prevent certain functional groups from undergoing undesired reactions. Typical protecting groups are described in "Protective Groups in Organic Synthesis," 4 th Edition, PGM Wuts; T.W. Greene, John Wiley, 2007, and the references cited therein.

[0312] Scheme 1 [ka]

[0313] In some embodiments, provided herein are methods for preparing compounds defined as Formula (I), as shown in Scheme 1. Halogen-substituted compound 1-1 (X2 and X4 are halogen, X1 is OH or Cl, and X3 can be methylthiolyl) is converted to compound 1-2 under substitution conditions (e.g., HATU, DIEA when X1 is OH; DIEA, DCM when X1 is Cl), compound 1-2 is then converted to compound 1-3 under substitution conditions (e.g., NaH, THF), compound 1-3 is then converted to compound 1-4 under oxidation conditions (m-CPBA oxidation when LG is methylsulfonyl or methylsulfinyl), compound 1-4 is then converted to compound 1-5, followed by a substitution or coupling reaction (e.g., NaH, THF), or further conversion. Compound 1-5 undergoes a metal-catalyzed cross-coupling reaction such as Suzuki, Negishi, or Stille coupling (e.g., Pd(dtbpf)Cl, KPO, 1,4-dioxane, water for Suzuki coupling) to give compound 1-6 (wherein M can be a boronic acid, a boronic ester, a metal (e.g., Zn), tributyltin, etc.), and then compound 1-6 bearing the protected group is finally deprotected (e.g., TFA and DCM to deprotect the Boc group if PG1 and PG2 contain a Boc group; CsF and DMF to deprotect the TIPS group if PG1 and PG2 contain a TIPS group) to give compound defined as formula (I).

[0314] Scheme 2 [ka]

[0315] In some embodiments, provided herein are methods for preparing compounds defined as Formula (I), as shown in Scheme 2. Halogen-substituted compound 2-1 (X2 and X4 are halogen, X1 is OH or Cl, and X3 can be methylthiolyl) is converted to compound 2-2 under substitution conditions (e.g., NaH, THF), which is then converted to compound 2-3 under substitution conditions (e.g., HATU, DIEA when X1 is OH; DIEA, DCM when X1 is Cl), which is then converted to compound 2-4 under oxidation conditions (e.g., m-CPBA oxidation when LG is methylsulfonyl or methylsulfinyl), which is then converted to compound 2-5, followed by a substitution or coupling reaction (e.g., NaH, THF), which is then further oxidized. Compound 2-5 undergoes a metal-catalyzed cross-coupling reaction such as Suzuki, Negishi, or Stille coupling (e.g., Pd(dtbpf)Cl, KPO, 1,4-dioxane, water for Suzuki coupling) to give compound 2-6 (where M can be a boronic acid, a boronic ester, a metal (e.g., Zn), tributyltin, etc.), and then compound 2-6 bearing the protected group is finally deprotected (e.g., TFA and DCM to deprotect the Boc group if PG1 and PG2 contain Boc groups; CsF and DMF to deprotect the TIPS group if PG1 and PG2 contain TIPS groups) to give compound defined as formula (I).

[0316] Embodiments of the present invention may be more fully understood by reference to the detailed description and examples, which are intended to exemplify non-limiting embodiments. [Example]

[0317] The following examples are intended to be merely illustrative and should not be construed as limiting in any way. Unless otherwise specified, experimental methods in the examples described below are conventional. Unless otherwise specified, all reagents and materials are commercially available. All solvents and chemicals used are of analytical grade or chemical purity. All solvents are redistilled before use. All anhydrous solvents are prepared by standard or reference methods. Silica gel (100-200 mesh) for column chromatography and silica gel (GF254) for thin-layer chromatography (TLC) are commercially available from Tsingdao Haiyang Chemical Co., Ltd. or Yantai Chemical Co., Ltd., China. Unless otherwise specified, all elutions were performed with petroleum ether (60-90°C) / ethyl acetate (v / v) and visualized with a solution of iodine or molybdophosphate in ethanol. Unless otherwise specified, all extraction solvents were dried over anhydrous Na2SO4.

[0318] Unless otherwise stated, reactions described below were carried out under a positive pressure of nitrogen or argon or in anhydrous solvents using drying tubes, reaction flasks were fitted with rubber septa for the introduction of substrates and reagents via syringe, and glassware was oven-dried and / or heat-dried.

[0319] Unless otherwise stated, column chromatographic purifications were performed on a Biotage system (manufacturer: Dyax Corporation) equipped with a silica gel column or a SepPak silica cartridge (Waters), or on a Teledyne Isco Combiflash purification system using prepacked silica gel cartridges.

[0320] 1 H NMR spectra were recorded on a Varian instrument operating at 400 MHz or 500 MHz using TMS (tetramethylsilane) as the internal standard. 1H-NMR spectra were obtained using CDCl3, CD2Cl2, CD3OD, DO, d6-DMSO, d6-acetone, or (CD3)2CO as the solvent and tetramethylsilane (0.00 ppm) or residual solvent as the reference standard (CDCl3: 7.25 ppm; CD3OD: 3.31 ppm; DO: 4.79 ppm; d6-DMSO: 2.50 ppm; d6-acetone: 2.05; (CD3)2CO: 2.05). When peak multiplicities are reported, the following abbreviations are used: s (singlet), d (doublet), t (triplet), q (quartet), qn (quintet), sx (sextet), m (multiplet), br (broad line), dd (doublet of doublets), and dt (doublet of triplets). Coupling constants, when indicated, are reported in Hertz (Hz).

[0321] LC / MS data were recorded using an Agilent 1100, 1200 High Performance Liquid Chromatography-Ion Trap Mass Spectrometer (LC-MSD Trap) equipped with a diode array detector (DAD) detecting at 214 nm and 254 nm and an ion trap (ESI source). All compound names, except for reagent names, were generated by ChemDraw® 19.1.

[0322] The following abbreviations are used in the examples below: [Table A] [Table B] [Table C] [Table D]

[0323] compound synthesis

[0324] Example 1: 2-amino-7-fluoro-4-((S)-4-fluoro-2-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-8a,9,10,11-tetrahydro-8H-pyrrolo[2',1':3,4][1,4]oxazepino[5,6,7-de]quinazolin-5-yl)benzo[b]thiophene-3-carbonitrile [ka]

[0325] Step 1: (S)-Pyrrolidin-2-ylmethanol [ka]

[0326] (S)-tert-Butyl 2-(hydroxymethyl)pyrrolidine-1-carboxylate (4 g, 19.9 mmol) was placed in HCl (4 M in dioxane) and the mixture was stirred at room temperature for 2 hours until LC-MS showed that all starting material was consumed. The mixture was concentrated under reduced pressure, diluted with MTBE, and then filtered to give the title compound (2.2 g) as a solid. MS (ESI, m / e) [M+H] + 102.2.

[0327] Step 2: (S)-(1-(7-bromo-5,8-difluoro-2-(methylthio)quinazolin-4-yl)pyrrolidin-2-yl)methanol [ka]

[0328] A mixture of (S)-pyrrolidin-2-ylmethanol (610 mg, 6.0 mmol) and 7-bromo-5,8-difluoro-2-(methylthio)quinazolin-4-ol (2.0 g, 6.6 mmol), HATU (3.8 g, 9.9 mmol), DIEA (1.3 g, 9.9 mmol), and DCM (10 mL) was stirred at room temperature for 16 hours. The mixture was quenched with NH4Cl solution (10 mL, saturated aqueous solution) and extracted with DCM (20 mL * 3). The combined organic layers were dried over Na2SO4. The mixture was filtered, and the filtrate was concentrated to give a residue, which was then purified on a silica chromatography column (eluted with PE / EtOAc = 2 / 1) to give the title compound (610 mg). MS (ESI, m / e) [M+H] + 389.2.

[0329] Step 3: (S)-5-Bromo-4-fluoro-2-(methylthio)-8a,9,10,11-tetrahydro-8H-pyrrolo[2',1':3,4][1,4]oxazepino[5,6,7-de]quinazoline [ka]

[0330] To a mixture of (S)-(1-(7-bromo-5,8-difluoro-2-(methylthio)quinazolin-4-yl)pyrrolidin-2-yl)methanol (570 mg, 1.5 mmol) in THF, NaH (114 mg, 3.0 mmol) was added portionwise at 0° C. The mixture was stirred at room temperature for 16 hours. The mixture was quenched with NH4Cl (10 mL, saturated aqueous solution), extracted with DCM (20 mL*3), and the combined organic layer was dried over Na2SO4. The filtrate was concentrated and purified on a silica chromatography column (eluted with PE / EtOAc=1 / 1) to give the title compound (480 mg). MS (ESI, m / e) [M+H] + 370.2.

[0331] Step 4: (8aS)-5-Bromo-4-fluoro-2-(methylsulfinyl)-8a,9,10,11-tetrahydro-8H-pyrrolo[2',1':3,4][1,4]oxazepino[5,6,7-de]quinazoline [ka]

[0332] To a mixture of (S)-5-bromo-4-fluoro-2-(methylthio)-8a,9,10,11-tetrahydro-8H-pyrrolo[2',1':3,4][1,4]oxazepino[5,6,7-de]quinazoline (480 mg, 1.3 mmol) in DCM (10 mL) was added m-CPBA (321 mg, 1.3 mmol, 70%) in portions at 0 °C. The mixture was stirred at 0 °C for 2 h. The mixture was diluted with DCM (20 mL) and washed three times with aqueous NaHCO (2 M). The resulting organic layer was dried over NaSO and concentrated to give the crude product (480 mg), which was used in the next step without further purification. MS (ESI, m / e) [M+H] + 386.2.

[0333] Step 5: (S)-5-Bromo-4-fluoro-2-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-8a,9,10,11-tetrahydro-8H-pyrrolo[2',1':3,4][1,4]oxazepino[5,6,7-de]quinazoline [ka]

[0334] To a mixture of ((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methanol (480 mg, 1.3 mmol) in THF (5 mL) was added NaH (99 mg, 2.6 mmol) portionwise at 0° C. The mixture was stirred for 0.5 h, and then added portionwise to a solution of (8aS)-5-bromo-4-fluoro-2-(methylsulfinyl)-8a,9,10,11-tetrahydro-8H-pyrrolo[2′,1′:3,4][1,4]oxazepino[5,6,7-de]quinazoline (480 mg, 1.3 mmol) in THF (10 mL) at 0° C. The mixture was stirred at 50° C. for 2 h. The mixture was quenched with NH4Cl (sat. aq. solution), extracted with DCM (20 mL*3), and the combined organic layer was dried over Na2SO4. The filtrate was concentrated and purified by silica chromatography column (eluted with DCM / MeOH=10 / 1) to give the title compound (480 mg). MS (ESI, m / e) [M+H] + 481.4.

[0335] Step 6: tert-butyl (3-cyano-7-fluoro-4-((S)-4-fluoro-2-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-8a,9,10,11-tetrahydro-8H-pyrrolo[2',1':3,4][1,4]oxazepino[5,6,7-de]quinazolin-5-yl)benzo[b]thiophen-2-yl)carbamate [ka]

[0336] (S)-5-Bromo-4-fluoro-2-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-8a,9,10,11-tetrahydro-8H-pyrrolo[2',1':3,4][1,4]oxazepino[5,6,7-de]quinazoline (100 mg, 0.2 mmol), (3-cyano-7-fluoro-4-(4,4,5,5-tetramethyl- A mixture of tert-butyl 1,3,2-dioxaborolan-2-yl)benzo[b]thiophen-2-yl)carbamate (251 mg, 0.6 mmol), bis(diphenylphosphinophenyl)etherpalladium(II) dichloride (15 mg, 0.02 mmol), CsCO (195 mg, 0.6 mmol), and dioxane / water (5 mL, 9 / 1) was stirred at 100 °C for 4 hours. The mixture was cooled to room temperature and concentrated under reduced pressure. The residue was purified on a silica chromatography column (eluted with DCM / MeOH = 9 / 1) to give the title compound (120 mg). MS (ESI, m / e) [M+H] + 693.4.

[0337] Step 7: 2-amino-7-fluoro-4-((S)-4-fluoro-2-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-8a,9,10,11-tetrahydro-8H-pyrrolo[2',1':3,4][1,4]oxazepino[5,6,7-de]quinazolin-5-yl)benzo[b]thiophene-3-carbonitrile

[0338] To a mixture of tert-butyl (3-cyano-7-fluoro-4-((S)-4-fluoro-2-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-8a,9,10,11-tetrahydro-8H-pyrrolo[2',1':3,4][1,4]oxazepino[5,6,7-de]quinazolin-5-yl)benzo[b]thiophen-2-yl)carbamate (120 mg, 0.17 mmol) in dioxane (2 mL) was added HCl (2 mL, 4 M in dioxane) in one portion. The mixture was stirred at room temperature for 16 hours. The solvent was removed under reduced pressure, and the residue was purified by preparative HPLC to give the title product (54 mg). 1 H NMR (500MHz, DMSO-d6) δ 8.03(s, 2H), 7.25-7.21 (m, 1H),7.10-7.08 (m, 1H), 6.71-6.63(m, 1H), 5.42-5.21 (m, 1H),4.63-4.61 (m, 1H), 4.22-3.96(m, 4H), 3.82-3.80 (m, 2H),3.16-3.13 (m, 2H), 2.90-2.86(m, 1H), 2.28-2.13 (m, 2H),2.08-1.99 (m, 3H), 1.95-1.64(m, 5H). MS (ESI, m / e) [M+H] + 593.4.

[0339] Example 2: 5-ethynyl-6-fluoro-4-((R)-8-fluoro-6-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-2,3,12,12a-tetrahydro-1H-pyrrolo[2',1':3,4][1,4]oxazepino[5,6,7-de]quinazolin-9-yl)naphthalen-2-ol [ka]

[0340] Example 2 was prepared by a similar procedure as described in Example 1 to give the title product (3.17 mg). 1H NMR (500MHz, DMSO-d6) δ 10.11(s, 1H), 8.01-7.87 (m, 1H),7.48-7.44 (m, 1H), 7.35-7.32(m, 1H), 7.10-7.02 (m, 1H),6.68-6.45 (m, 1H), 5.38-5.20(m, 1H), 4.65-4.56 (m, 1H),4.12-4.08 (m, 3H), 3.98-3.94(m, 2H), 3.84-3.82 (m, 2H),3.11-3.00 (m, 3H), 2.84-2.81(m, 1H), 2.36-2.16(m, 2H),2.15-2.07 (m, 2H), 2.06-1.97(m, 3H), 1.91-1.82 (m, 2H),1.80-1.74 (m, 2H). MS (ESI, m / e) [M+H] + 587.6.

[0341] Example 3: 5-ethynyl-6-fluoro-4-((R)-8-fluoro-6-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-1,2,3,12,13,13a-hexahydropyrrolo[1′,2′:5,6][1,5]oxazocino[4,3,2-de]quinazolin-9-yl)naphthalen-2-ol [ka]

[0342] Example 3 was prepared by a similar procedure as described in Example 1 to give the title product (0.78 mg). 1H NMR (500MHz, DMSO-d6) δ 10.13(s, 1H), 7.96 (s,1H), 7.51-7.42 (m, 1H),7.35 (s, 1H), 7.11-7.01(m, 1H), 6.76 (s,1H), 5.63-5.40 (m, 1H),4.65-4.45 (m, 3H), 4.34-4.10(m, 3H), 4.03-3.93 (m, 1H),3.88-3.68 (m, 6H), 2.20-2.10(m, 4H), 2.05-1.95 (m, 4H),1.94-1.85 (m, 3H). MS (ESI, m / e) [M+H] + 601.6.

[0343] Example 4: 5-ethynyl-6-fluoro-4-((S)-2,2,8-trifluoro-6-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-2,3,12,12a-tetrahydro-1H-pyrrolo[2',1':3,4][1,4]oxazepino[5,6,7-de]quinazolin-9-yl)naphthalen-2-ol [ka]

[0344] Example 4 was prepared by a similar procedure as described in Example 1 by replacing 3-(methylamino)propan-1-ol with (S)-(4,4-difluoropyrrolidin-2-yl)methanol to afford the title product (2.25 mg). 1H NMR (500MHz, DMSO-d6) δ 10.13(s, 1H), 7.98-7.92 (m, 1H),7.53-7.40 (m, 1H), 7.37-7.31(m, 1H), 7.09-7.02 (m, 1H),6.83-6.70 (m, 1H), 5.38-5.15(m, 1H), 4.70-3.89 (m, 8H),3.12-3.06 (m, 2H), 3.04-3.01(m, 1H), 2.86-2.69 (m, 2H),2.14-2.07 (m, 1H), 2.05-1.99(m, 2H), 1.87-1.82(m, 1H),1.80-1.72 (m, 2H). MS (ESI, m / e) [M+H] + 623.3.

[0345] Example 5: 2-amino-7-fluoro-4-((R)-8-fluoro-6-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-1,2,3,12,13,13a-hexahydropyrrolo[1′,2′:5,6][1,5]oxazocino[4,3,2-de]quinazolin-9-yl)benzo[b]thiophene-3-carbonitrile [ka]

[0346] Example 5 was prepared by a procedure similar to that described in Example 1 by replacing (S)-5-bromo-4-fluoro-2-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-8a,9,10,11-tetrahydro-8H-pyrrolo[2',1':3,4][1,4]oxazepino[5,6,7-de]quinazoline with (R)-9-bromo-8-fluoro-6-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-1,2,3,12,13,13a-hexahydropyrrolo[1',2':5,6][1,5]oxazocino[4,3,2-de]quinazoline to provide the title product (58 mg). 1H NMR (500MHz, DMSO-d6) δ 8.10-8.03(m, 2H), 7.31-7.15 (m, 1H),7.15-7.05 (m, 1H), 6.70-6.62(m, 1H), 5.41-5.20 (m, 1H),4.48-4.40 (m, 1H), 4.28-3.99(m, 4H), 3.84-3.80 (m, 1H),3.73-3.63 (m, 1H), 3.20-3.10(m, 3H), 2.89 (s,1H), 2.23-1.94 (m, 6H),1.93-1.74 (m, 5H). MS (ESI, m / e) [M+H] + 607.1.

[0347] Example 6: 5-ethynyl-6-fluoro-4-(1-fluoro-13-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-6,6a,7,8,9,10-hexahydro-5H-pyrido[1′,2′:5,6][1,5]oxazocino[4,3,2-de]quinazolin-2-yl)naphthalen-2-ol [ka]

[0348] Step 1: 7-Bromo-8-fluoro-2-(methylthio)-5-(2-(piperidin-2-yl)ethoxy)quinazolin-4(3H)-one [ka]

[0349] To a solution of tert-butyl 2-(2-hydroxyethyl)piperidine-1-carboxylate (2.2 g, 9.8 mmol) in THF (50 mL) was added NaH (60%, 536 mg, 13.4 mol). The mixture was stirred at room temperature for 10 minutes, and then 7-bromo-5,8-difluoro-2-(methylthio)quinazolin-4(3H)-one (2.0 g, 7.5 mol) was added to the mixture. The resulting mixture was stirred at 55 °C overnight. The mixture was cooled to room temperature and quenched with ice. The mixture was extracted with DCM (30 mL * 3) and washed with brine (50 mL). The organic phase was dried over Na2SO4 and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (20 g, eluted with PE / EtOAc = 100%:0% to 0%:100%) to give the title compound (1.2 g). MS (ESI, m / e) [M+H] + 416.2.

[0350] Step 2: 2-Bromo-1-fluoro-13-(methylthio)-6,6a,7,8,9,10-hexahydro-5H-pyrido[1',2':5,6][1,5]oxazocino[4,3,2-de]quinazoline [ka]

[0351] To a suspension of 7-bromo-8-fluoro-2-(methylthio)-5-(2-(piperidin-2-yl)ethoxy)quinazolin-4(3H)-one (1.2 g, 2.9 mmol) in DCM / DMF (20 mL / 20 mL) was added HATU (2.9 g, 7.3 mmol) and DIEA (1.1 g, 8.7 mmol). The mixture was stirred at room temperature overnight. The mixture was then extracted with DCM (30 mL*2) and washed with brine (50 mL). The combined organic layers were dried over Na2SO4 and concentrated under reduced pressure. The mixture was purified by silica chromatography column (20 g, eluted with PE / EtOAc = 100%:0% to 65%:35%) to give the title compound (650 mg). MS (ESI, m / e) [M+H] + 398.2.

[0352] Step 3: 2-Bromo-1-fluoro-13-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-6,6a,7,8,9,10-hexahydro-5H-pyrido[1',2':5,6][1,5]oxazocino[4,3,2-de]quinazoline [ka]

[0353] 2-Bromo-1-fluoro-13-(methylthio)-6,6a,7,8,9,10-hexahydro-5H-pyrido[1',2':5,6][1,5]oxazocino[4,3,2-de]quinazoline (620 mg, 1.56 mmol) was placed in DCM (10 mL). m-CPBA (309 mg, 1.796 mmol) was added portionwise to the solution at 0 °C. The mixture was stirred at 0 °C for 1 h. The mixture was then extracted with DCM (10 mL * 2), washed with Na2CO3 (20 mL, saturated aqueous solution), and washed with brine (20 mL). The combined organic layers were dried over Na2SO4 and concentrated under reduced pressure to give the crude product, which was used directly. ((2R,7aS)-2-Fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methanol (744 mg, 4.7 mmol) was placed in THF (10 mL), and LiHMDS (3.1 mL, 3.1 mmol) was added portionwise to the solution at 0° C. The mixture was stirred at 0° C. for 1 hour, and the above crude product was added. The mixture was stirred from 0° C. to room temperature and stirred at room temperature for 1 hour. The mixture was then quenched with MeOH (5 mL) and concentrated under reduced pressure. The residue was purified by silica chromatography column (eluted with DCM / MeOH=80%:20%) to give the title compound (213 mg). MS (ESI, m / e) [M+H] + 509.3.

[0354] Step 4: 1-fluoro-2-(7-fluoro-3-(methoxymethoxy)-8-((triisopropylsilyl)ethynyl)naphthalen-1-yl)-13-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-6,6a,7,8,9,10-hexahydro-5H-pyrido[1',2':5,6][1,5]oxazocino[4,3,2-de]quinazoline [ka]

[0355] 2-Bromo-1-fluoro-13-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-6,6a,7,8,9,10-hexahydro-5H-pyrido[1',2':5,6][1,5]oxazocino[4,3,2-de]quinazoline (100 mg, 0.2 mmol), ((2-fluoro-6-(methoxymethoxy) A mixture of 8-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)naphthalen-1-yl)ethynyl)triisopropylsilane (302 mg, 0.6 mmol), 1,1'-bis(diphenylphosphino)ferrocene-palladium(II) dichloromethane dichloromethane complex (18 mg, 0.03 mmol), and NaHCO3 (50 mg, 0.6 mmol) was stirred at 100 °C overnight. The cooled reaction mixture was concentrated under reduced pressure, and the residue was purified by silica chromatography column (4 g, eluted with PE / EtOAc = 100%:0% to 0%:100%, DCM / MeOH = 90%:10%) to give the title compound (140 mg). MS (ESI, m / e) [M+H] + 815.7.

[0356] Step 5: 2-(8-ethynyl-7-fluoro-3-(methoxymethoxy)naphthalen-1-yl)-1-fluoro-13-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-6,6a,7,8,9,10-hexahydro-5H-pyrido[1',2':5,6][1,5]oxazocino[4,3,2-de]quinazoline [ka] To a solution of 1-fluoro-2-(7-fluoro-3-(methoxymethoxy)-8-((triisopropylsilyl)ethynyl)naphthalen-1-yl)-13-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-6,6a,7,8,9,10-hexahydro-5H-pyrido[1',2':5,6][1,5]oxazocino[4,3,2-de]quinazoline (70 mg, 0.09 mmol) in DMF (5 mL) was added CsF (65 mg, 0.4 mmol). The mixture was stirred overnight at room temperature. The mixture was then filtered, and the filtrate was concentrated under reduced pressure to give the crude product, which was used in the next step without further purification (80 mg, crude). MS (ESI, m / e) [M+H] + 659.5.

[0357] Step 6: 5-ethynyl-6-fluoro-4-(1-fluoro-13-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-6,6a,7,8,9,10-hexahydro-5H-pyrido[1',2':5,6][1,5]oxazocino[4,3,2-de]quinazolin-2-yl)naphthalen-2-ol

[0358] To a solution of 2-(8-ethynyl-7-fluoro-3-(methoxymethoxy)naphthalen-1-yl)-1-fluoro-13-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-6,6a,7,8,9,10-hexahydro-5H-pyrido[1',2':5,6][1,5]oxazocino[4,3,2-de]quinazoline (80 mg, 0.1 mmol) in dioxane (4 mL) was added HCl (2 mL, 4 M in dioxane). The mixture was stirred at room temperature for 2 hours. The reaction mixture was concentrated under reduced pressure, and the residue was purified by preparative HPLC to give the title product (22 mg). 1 H NMR (500MHz, DMSO-d6) δ 10.14(br, 1H), 7.96-7.92 (m, 1H),7.44-7.40 (m, 1H), 7.36-7.30(m, 1H), 7.13-6.96 (m, 1H),6.69-6.57 (m, 1H), 5.36-5.18(m, 1H), 4.95-4.65 (m, 1H),4.38-4.26 (m, 1H), 4.17-4.01(m, 2H), 3.99-3.87 (m, 3H),3.13-3.05 (m, 2H), 3.04-3.00(m, 1H), 2.84-2.80(m, 1H), 2.34-2.30 (m, 1H), 2.20-1.97 (m, 3H), 1.92-1.49(m, 10H). MS (ESI, m / e) [M+H] + 615.5.

[0359] Example 7: 2-amino-4-((13aR)-10-chloro-8-fluoro-6-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-1,2,3,12,13,13a-hexahydropyrrolo[1′,2′:5,6][1,5]oxazocino[4,3,2-de]quinazolin-9-yl)-7-fluorobenzo[b]thiophene-3-carbonitrile [ka]

[0360] Step 1: 7-Bromo-4,6-dichloro-5,8-difluoro-2-(methylthio)quinazoline [ka]

[0361] To a suspension of 7-bromo-6-chloro-5,8-difluoro-2-(methylthio)quinazolin-4-ol (900 mg, 2.8 mmol) in POCl3 (10 mL) was added dropwise DIPEA (5 mL). The mixture was stirred at 100 °C overnight. The mixture was then cooled to room temperature and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (20 g, eluted with PE / EtOAc = 100%:0% to 70%:30%) to give the title compound (700 mg). MS (ESI, m / e) [M+H] + 359.0.

[0362] Step 2: (R)-2-(1-(7-bromo-6-chloro-5,8-difluoro-2-(methylthio)quinazolin-4-yl)pyrrolidin-2-yl)ethan-1-ol [ka]

[0363] To a solution of 7-bromo-4,6-dichloro-5,8-difluoro-2-(methylthio)quinazoline (700 mg, 2.0 mmol) and (R)-2-(pyrrolidin-2-yl)ethan-1-ol (337 mg, 2.9 mmol) in DCM (20 mL) was added DIPEA (756 mg, 5.9 mmol). The mixture was stirred from 0 °C to room temperature for 1 hour. The mixture was then concentrated under reduced pressure, and the residue was purified by silica gel column chromatography (12 g, eluted with PE / EtOAc = 100%:0% to 0%:100%) to give the title compound (600 mg). MS (ESI, m / e) [M+H] + 438.1.

[0364] Step 3: (R)-9-Bromo-10-chloro-8-fluoro-6-(methylthio)-1,2,3,12,13,13a-hexahydropyrrolo[1',2':5,6][1,5]oxazocino[4,3,2-de]quinazoline [ka]

[0365] To a solution of (R)-2-(1-(7-bromo-6-chloro-5,8-difluoro-2-(methylthio)quinazolin-4-yl)pyrrolidin-2-yl)ethan-1-ol (600 mg, 1.4 mmol) in THF, LiHMDS (2.8 mL, 2.8 mmol, 1 M in THF) was added portionwise at 0° C. The mixture was stirred from 0° C. to room temperature and stirred at room temperature for 1 hour. The mixture was quenched with MeOH (5 mL), and then the mixture was concentrated under reduced pressure. The residue was purified by silica gel column chromatography (12 g, eluted with PE / EtOAc = 100%:0% to 0%:100%) to give the title compound (540 mg). MS (ESI, m / e) [M+H] + 418.7.

[0366] Step 4: (R)-9-Bromo-10-chloro-8-fluoro-6-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-1,2,3,12,13,13a-hexahydropyrrolo[1',2':5,6][1,5]oxazocino[4,3,2-de]quinazoline [ka]

[0367] To a mixture of (R)-9-bromo-10-chloro-8-fluoro-6-(methylthio)-1,2,3,12,13,13a-hexahydropyrrolo[1',2':5,6][1,5]oxazocino[4,3,2-de]quinazoline (540 mg, 1.3 mmol) in DCM (10 mL) was added m-CPBA (256 mg, 1.5 mmol, 70%) in portions at 0 °C. The mixture was stirred at 0 °C for 2 hours to give mixture A. To a mixture of ((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methanol (617 mg, 3.385 mmol) in THF (10 mL) was added LiHMDS (2.6 mL, 2.6 mmol) in portions at 0 °C. The mixture was stirred at room temperature for 30 minutes and added to the above mixture A. The resulting mixture was stirred at room temperature for 2 hours, quenched with MeOH (5 mL), and concentrated under reduced pressure. The residue was purified by silica chromatography column (eluted with DCM / MeOH=90%:10%) to give the title compound (500 mg). MS (ESI, m / e) [M+H] + 529.3.

[0368] Step 5: tert-butyl (4-((13aR)-10-chloro-8-fluoro-6-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-1,2,3,12,13,13a-hexahydropyrrolo[1',2':5,6][1,5]oxazocino[4,3,2-de]quinazolin-9-yl)-3-cyano-7-fluorobenzo[b]thiophen-2-yl)carbamate [ka]

[0369] (R)-9-Bromo-10-chloro-8-fluoro-6-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-1,2,3,12,13,13a-hexahydropyrrolo[1',2':5,6][1,5]oxazocino[4,3,2-de]quinazoline (70 mg, 0.132 mmol), (3-cyano-7- A mixture of tert-butyl fluoro-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)benzo[b]thiophen-2-yl)carbamate (220 mg, 0.53 mmol), bis(diphenylphosphinophenyl)etherpalladium(II) dichloride (19 mg, 0.026 mmol), and CsCO (171 mg, 0.53 mmol) was stirred at 100 °C overnight. The mixture was cooled to room temperature and concentrated under reduced pressure. The residue was purified by silica chromatography column (4 g, eluted with DCM / MeOH = 90%:10%) to give the crude product, which was further purified by preparative TLC (DCM / MeOH = 20 / 1 and EtOAc) to give the title compound (25 mg, crude). MS (ESI, m / e) [M+H] + 741.5.

[0370] Step 6: 2-amino-4-((13aR)-10-chloro-8-fluoro-6-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-1,2,3,12,13,13a-hexahydropyrrolo[1',2':5,6][1,5]oxazocino[4,3,2-de]quinazolin-9-yl)-7-fluorobenzo[b]thiophene-3-carbonitrile

[0371] To a solution of tert-butyl (4-((13aR)-10-chloro-8-fluoro-6-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-1,2,3,12,13,13a-hexahydropyrrolo[1',2':5,6][1,5]oxazocino[4,3,2-de]quinazolin-9-yl)-3-cyano-7-fluorobenzo[b]thiophen-2-yl)carbamate (25 mg, 0.034 mmol) in dioxane (5 mL) was added HCl (4 M) / dioxane (5 mL). The mixture was stirred at room temperature for 3 hours. The mixture was then concentrated under reduced pressure to give the crude product, which was further purified by preparative HPLC to give the desired product (0.99 mg). 1 H NMR (500MHz, DMSO-d6) δ 8.06(s, 2H), 7.26-7.05 (m, 2H),5.36-5.18 (m, 1H), 4.54-5.38(m, 1H), 4.26-4.13 (m, 2H),4.10-3.98 (m, 2H), 3.87-3.67(m, 2H), 3.14-2.98 (m, 3H),2.84-2.80 (m, 1H), 2.18-2.10(m, 6H), 1.80-1.71 (m, 4H). MS(ESI, m / e) [M+H] + 641.5.

[0372] Example 8: 3-chloro-5-(1-fluoro-12-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-6,6a,7,8,9,10-hexahydro-5H-4-oxa-3,10a,11,13-tetraazabenzo[4,5]cycloocta[1,2,3-de]naphthalen-2-yl)-4-(trifluoromethyl)aniline [ka]

[0373] Step 1: 7-chloro-8-fluoro-2-(methylthio)-5-(2-(piperidin-2-yl)ethoxy)pyrido[4,3-d]pyrimidin-4-ol [ka]

[0374] To a solution of 2-(piperidin-2-yl)ethan-1-ol (129 mg, 1 mmol) in THF (10 mL) was added NaH (40 mg, 1 mmol) at room temperature. The resulting mixture was stirred at room temperature for 1 hour. Then, 5,7-dichloro-8-fluoro-2-(methylthio)pyrido[4,3-d]pyrimidin-4-ol (280 mg, 1 mmol) was added to the reaction mixture and stirred at room temperature for 1 hour. After stirring was complete, the reaction mixture was concentrated to give a residue. The residue was purified by flash silica column (DCM:MeOH=10:1) to give the title product (240 mg). MS (ESI, m / e) [M+H] + 373.1.

[0375] Step 2: 2-chloro-1-fluoro-12-(methylthio)-6,6a,7,8,9,10-hexahydro-5H-4-oxa-3,10a,11,13-tetraazabenzo[4,5]cycloocta[1,2,3-de]naphthalene [ka]

[0376] To a solution of 7-chloro-8-fluoro-2-(methylthio)-5-(2-(piperidin-2-yl)ethoxy)pyrido[4,3-d]pyrimidin-4-ol (240 mg, 0.645 mmol) in 20 mL of N,N-dimethylformamide, N,N-diisopropylethylamine (166.4 mg, 1.29 mmol) and 2-(7-azabenzotriazol-1-yl)-N,N,N',N'-tetramethyluronium hexafluorophosphate (490.2 mg, 1.29 mmol) were added at room temperature, and the mixture was stirred at room temperature for 1 hour. Additional N,N-diisopropylethylamine (1.66 g, 12.9 mmol) and 2-(7-azabenzotriazol-1-yl)-N,N,N',N'-tetramethyluronium hexafluorophosphate (4.9 g, 12.9 mmol) were added, and the mixture was stirred at room temperature for 2 hours. The reaction mixture was then diluted with water and extracted with EA. The organic layers were combined, dried over sodium sulfate, and evaporated. The residue was purified by silica chromatography (PE: EtOAc = 1:5) to give the title product (75 mg). MS (ESI, m / e) [M+H] + 355.1.

[0377] Step 3: 2-chloro-1-fluoro-12-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-6,6a,7,8,9,10-hexahydro-5H-4-oxa-3,10a,11,13-tetraazabenzo[4,5]cycloocta[1,2,3-de]naphthalene [ka]

[0378] To a solution of 2-chloro-1-fluoro-12-(methylthio)-6,6a,7,8,9,10-hexahydro-5H-4-oxa-3,10a,11,13-tetraazabenzo[4,5]cycloocta[1,2,3-de]naphthalene (75 mg, 0.212 mmol) in 10 mL of dichloromethane, 3-chloroperbenzoic acid (44 mg, 0.254 mmol) was added at room temperature, and the mixture was stirred at room temperature for 2 hours as mixture 1. Meanwhile, LiHMDS (1N in THF, 0.3 mL) was added to ((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methanol (101 mg, 0.635 mmol) in THF (10 mL) at room temperature, and the mixture was stirred at room temperature for 2 hours as mixture 2. Then, mixture 2 was added to mixture 1 at room temperature, and the mixture was stirred at room temperature for 2 hours. The solvent was then evaporated, and the residue was purified by silica chromatography column (DCM:MeOH=10:1) to give the title product (73 mg). MS (ESI, m / e) [M+H] + 466.2.

[0379] Step 4: 3-chloro-5-(1-fluoro-12-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-6,6a,7,8,9,10-hexahydro-5H-4-oxa-3,10a,11,13-tetraazabenzo[4,5]cycloocta[1,2,3-de]naphthalen-2-yl)-4-(trifluoromethyl)aniline

[0380] 2-Chloro-1-fluoro-12-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-6,6a,7,8,9,10-hexahydro-5H-4-oxa-3,10a,11,13-tetraazabenzo[4,5]cycloocta[1,2,3-de]naphthalene (73m) in dioxane (10 mL) and water (2 mL) A mixture of 3-chloro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-4-(trifluoromethyl)aniline (101 mg, 0.314 mmol), KPO (66.6 mg, 0.314 mmol), and Pd(dtbpf)Cl (20.4 mg, 0.0314 mmol) was stirred at 95° C. for 3 hours. The resulting mixture was cooled and concentrated, and the residue was purified by column chromatography (DCM / MeOH=10 / 1) to give the crude product, which was further purified by preparative HPLC to give the title product (1.75 mg). 1 H NMR (500MHz, CD3OD) δ 6.89- 6.85 (m, 1H),6.60 - 6.36 (m,1H), 5.40 - 5.26(m, 2H), 4.55 -4.48 (m, 1H), 4.39- 4.16 (m, 3H),4.13 - 4.01 (m,1H), 3.18 - 3.03(m, 2H), 2.49 -1.56 (m, 16H). MS (ESI, m / e) [M+H] + 625.2.

[0381] Example 9: 3-chloro-5-((S)-7-fluoro-5-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-2,3,11,11a-tetrahydro-1H-10-oxa-3a,4,6,9-tetraazanaphtho[1,8-ef]azulen-8-yl)-4-(trifluoromethyl)aniline [ka]

[0382] Step 1: 4,5,7-trichloro-8-fluoro-2-(methylthio)pyrido[4,3-d]pyrimidine [ka]

[0383] To a solution of 5,7-dichloro-8-fluoro-2-(methylthio)pyrido[4,3-d]pyrimidin-4-ol (280 mg, 1.0 mmol) in acetonitrile (10 mL) was added DIPEA (194 mg, 1.5 mmol) and POCl (184 mg, 1.2 mmol), and the resulting mixture was stirred at 80° C. for 2 h. The reaction mixture was concentrated under reduced pressure and used in the next step without further purification.

[0384] Step 2: (S)-(1-(5,7-dichloro-8-fluoro-2-(methylthio)pyrido[4,3-d]pyrimidin-4-yl)pyrrolidin-2-yl)methanol [ka]

[0385] To a stirred solution of 4,5,7-trichloro-8-fluoro-2-(methylthio)pyrido[4,3-d]pyrimidine (1.0 mmol) in CHCl (10 mL) was added DIPEA (387 mg, 3.0 mmol) and (S)-pyrrolidin-2-ylmethanol (101 mg, 1.0 mmol) at 0 °C, and the resulting mixture was stirred at room temperature for 30 min. The reaction mixture was concentrated and purified by flash chromatography (PE / EtOAc = 4:1 to 1:2) to give the desired product (210 mg). MS (ESI, m / e) [M+H] + 363.1.

[0386] Step 3: (S)-8-chloro-7-fluoro-5-(methylthio)-2,3,11,11a-tetrahydro-1H-10-oxa-3a,4,6,9-tetraazanaphtho[1,8-ef]azulene [ka]

[0387] To a stirred solution of (S)-(1-(5,7-dichloro-8-fluoro-2-(methylthio)pyrido[4,3-d]pyrimidin-4-yl)pyrrolidin-2-yl)methanol (162 mg, 0.45 mmol) in THF (5 mL) was added LiHMDS (1 mL, 1 mmol, 1 M in THF) at 0 °C, and the resulting mixture was stirred at room temperature for 2 h. The reaction was quenched with HO and then extracted with EtOAc. The combined organic layers were washed with brine, dried over anhydrous NaSO, and concentrated under reduced pressure. The residue was purified by flash chromatography (PE / EtOAc = 10:1 to 2:1) to give the product (104 mg). MS (ESI, m / e) [M+H] + 327.0.

[0388] Step 4: (11aS)-8-chloro-7-fluoro-5-(methylsulfinyl)-2,3,11,11a-tetrahydro-1H-10-oxa-3a,4,6,9-tetraazanaphtho[1,8-ef]azulene [ka]

[0389] To a stirred solution of (S)-8-chloro-7-fluoro-5-(methylthio)-2,3,11,11a-tetrahydro-1H-10-oxa-3a,4,6,9-tetraazanaphtho[1,8-ef]azulene (104 mg, 0.32 mmol) in CHCl (2.5 mL) was added m-CPBA (78 mg, 0.38 mmol, 85%) at 0° C., and the resulting mixture was stirred at 0° C. for 10 min. The reaction mixture was used in the next step without further purification. MS (ESI, m / e) [M+H] + 343.3.

[0390] Step 5: (S)-8-chloro-7-fluoro-5-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-2,3,11,11a-tetrahydro-1H-10-oxa-3a,4,6,9-tetraazanaphtho[1,8-ef]azulene [ka]

[0391] To a stirred solution of (11aS)-8-chloro-7-fluoro-5-(methylsulfinyl)-2,3,11,11a-tetrahydro-1H-10-oxa-3a,4,6,9-tetraazanaphtho[1,8-ef]azulene (0.32 mmol), ((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methanol (102 mg, 0.64 mmol) in THF (2.5 mL) and LiHMDS (0.96 mL, 0.96 mmol, 1 M in THF) was added dropwise at 0 °C, and the resulting mixture was stirred at 0 °C for 20 min. The reaction was quenched with HO and concentrated under reduced pressure. The residue was purified by flash chromatography (CHCl / MeOH = 100:1 to 30:1) to give the product (92 mg). MS (ESI, m / e) [M+H] + 438.5.

[0392] Step 6: 3-chloro-5-((S)-7-fluoro-5-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-2,3,11,11a-tetrahydro-1H-10-oxa-3a,4,6,9-tetraazanaphtho[1,8-ef]azulen-8-yl)-4-(trifluoromethyl)aniline

[0393] A mixture of (S)-8-chloro-7-fluoro-5-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-2,3,11,11a-tetrahydro-1H-10-oxa-3a,4,6,9-tetraazanaphtho[1,8-ef]azulene (30 mg, 0.07 mmol), 3-chloro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-4-(trifluoromethyl)aniline (50 mg, 0.14 mmol, 89%), Pd(dtbpf)Cl (9.0 mg, 0.014 mmol), NaHCO (18 mg, 0.21 mmol), dioxane (5.0 mL), and HO (1.0 mL) was stirred at 90 °C for 3 h. The reaction mixture was concentrated and purified by flash chromatography (CH2Cl2 / MeOH=100:1 to 5:1), and then further purified by preparative HPLC to give the title product (12.2 mg). 1 H NMR (500MHz, CD3OD) δ 6.92- 6.83 (m, 1H),6.58 - 6.40 (m,1H), 5.52 - 5.25(m, 1H), 4.77 -4.70 (m, 2H), 4.50- 3.85 (m, 6H),3.52 - 3.40 (m,1H), 3.20 - 3.03(m, 2H), 2.43 -1.75 (m, 10H). MS (ESI, m / e) [M+H] + 597.4.

[0394] Example 10: 5-ethynyl-6-fluoro-4-((S)-7-fluoro-5-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-2,3,11,11a-tetrahydro-1H-10-oxa-3a,4,6,9-tetraazanaphtho[1,8-ef]azulen-8-yl)naphthalen-2-ol [ka]

[0395] Step 1: (S)-7-fluoro-8-(7-fluoro-3-(methoxymethoxy)-8-((triisopropylsilyl)ethynyl)naphthalen-1-yl)-5-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-2,3,11,11a-tetrahydro-1H-10-oxa-3a,4,6,9-tetraazanaphtho[1,8-ef]azulene [ka]

[0396] (S)-8-chloro-7-fluoro-5-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-2,3,11,11a-tetrahydro-1H-10-oxa-3a,4,6,9-tetraazanaphtho[1,8-ef]azulene (50 mg, 0.11 mmol), ((2-fluoro-6-(methoxymethoxy)-8-(4,4 A mixture of 5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)naphthalen-1-yl)ethynyl)triisopropylsilane (113 mg, 0.22 mmol), Pd(dtbpf)Cl (14 mg, 0.022 mmol), NaHCO (28 mg, 0.33 mmol), dioxane (5.0 mL), and HO (1.0 mL) was stirred at 90 °C for 2 h. The reaction mixture was concentrated and purified by flash chromatography (CHCl / MeOH = 100:1 to 10:1) to give the title product (65 mg). MS (ESI, m / e) [M+H] + 788.8.

[0397] Step 2: (S)-8-(8-ethynyl-7-fluoro-3-(methoxymethoxy)naphthalen-1-yl)-7-fluoro-5-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-2,3,11,11a-tetrahydro-1H-10-oxa-3a,4,6,9-tetraazanaphtho[1,8-ef]azulene [ka]

[0398] To a stirred solution of (S)-7-fluoro-8-(7-fluoro-3-(methoxymethoxy)-8-((triisopropylsilyl)ethynyl)naphthalen-1-yl)-5-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-2,3,11,11a-tetrahydro-1H-10-oxa-3a,4,6,9-tetraazanaphtho[1,8-ef]azulene (65 mg, 0.08 mmol) in DMF (4 mL) was added CsF (122 mg, 0.8 mmol), and the resulting mixture was stirred for 2 h. The reaction mixture was concentrated under reduced pressure, and the crude product was used directly in the next step without further purification. MS (ESI, m / e) [M+H] + 632.5.

[0399] Step 3: 5-ethynyl-6-fluoro-4-((S)-7-fluoro-5-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-2,3,11,11a-tetrahydro-1H-10-oxa-3a,4,6,9-tetraazanaphtho[1,8-ef]azulen-8-yl)naphthalen-2-ol

[0400] To a stirred solution of (S)-8-(8-ethynyl-7-fluoro-3-(methoxymethoxy)naphthalen-1-yl)-7-fluoro-5-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-2,3,11,11a-tetrahydro-1H-10-oxa-3a,4,6,9-tetraazanaphtho[1,8-ef]azulene (0.08 mmol) in CHCl (3.0 mL) was added 4 N HCl in dioxane (0.5 mL) at 0 °C, and the resulting mixture was stirred at room temperature for 1 h. The reaction mixture was concentrated under reduced pressure, and the residue was purified by flash chromatography (CHCl / MeOH = 100:1 to 10:1) and then further purified by preparative HPLC to give the title product (3.5 mg). 1H NMR (500MHz, CD3OD) δ 7.92- 7.72 (m, 1H),7.41 - 7.09 (m,3H), 5.49 - 5.29(m, 1H), 4.79 -4.71 (m, 1H), 4.50- 3.87 (m, 6H),3.62 - 3.33 (m,5H), 2.53 - 1.78(m, 10H). MS (ESI, m / e) [M+H] + 588.4.

[0401] Example 11: 3-chloro-5-((R)-7-fluoro-5-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-1,2,3,11,12,12a-hexahydro-10-oxa-3a,4,6,9-tetraazacyclopenta[4,5]cycloocta[1,2,3-de]naphthalen-8-yl)-4-(trifluoromethyl)aniline [ka]

[0402] Step 1: 4,5,7-trichloro-8-fluoro-2-(methylthio)pyrido[4,3-d]pyrimidine [ka]

[0403] To a solution of 5,7-dichloro-8-fluoro-2-(methylthio)pyrido[4,3-d]pyrimidin-4-ol (280 mg, 1.0 mmol) in acetonitrile (10 mL) was added DIPEA (194 mg, 1.5 mmol) and POCl (184 mg, 1.2 mmol), and the resulting mixture was stirred at 80° C. for 2 h. The reaction mixture was concentrated under reduced pressure and used directly in the next step without further purification.

[0404] Step 2: (R)-2-(1-(5,7-dichloro-8-fluoro-2-(methylthio)pyrido[4,3-d]pyrimidin-4-yl)pyrrolidin-2-yl)ethan-1-ol [ka]

[0405] To a stirred solution of 4,5,7-trichloro-8-fluoro-2-(methylthio)pyrido[4,3-d]pyrimidine (1.0 mmol) in CHCl (5 mL) was added DIPEA (1.94 mg, 1.5 mmol) and (R)-2-(pyrrolidin-2-yl)ethan-1-ol hydrochloride (11 mg, 1.0 mmol) at 0 °C, and the resulting mixture was stirred at room temperature for 30 min. The reaction mixture was concentrated and purified by flash chromatography (PE / EtOAc = 4:1 to 1:2) to give the desired product (262 mg). MS (ESI, m / e) [M+H] + 377.1.

[0406] Step 3: (R)-8-chloro-7-fluoro-5-(methylthio)-1,2,3,11,12,12a-hexahydro-10-oxa-3a,4,6,9-tetraazacyclopenta[4,5]cycloocta[1,2,3-de]naphthalene [ka]

[0407] To a stirred solution of (R)-2-(1-(5,7-dichloro-8-fluoro-2-(methylthio)pyrido[4,3-d]pyrimidin-4-yl)pyrrolidin-2-yl)ethan-1-ol (262 mg, 0.70 mmol) in THF (5 mL) was added LiHMDS (1 mL, 1 mmol, 1 M in THF) at 0 °C, and the resulting mixture was stirred at room temperature for 2 h. The reaction was quenched with HO and concentrated under reduced pressure. The residue was purified by flash chromatography (PE / EtOAc = 10:1 to 2:1) to give the product (210 mg). MS (ESI, m / e) [M+H] + 340.8.

[0408] Step 4: (12aR)-8-chloro-7-fluoro-5-(methylsulfinyl)-1,2,3,11,12,12a-hexahydro-10-oxa-3a,4,6,9-tetraazacyclopenta[4,5]cycloocta[1,2,3-de]naphthalene [ka]

[0409] To a stirred solution of (R)-8-chloro-7-fluoro-5-(methylthio)-1,2,3,11,12,12a-hexahydro-10-oxa-3a,4,6,9-tetraazacyclopenta[4,5]cycloocta[1,2,3-de]naphthalene (210 mg, 0.62 mmol) in CHCl (5 mL) was added m-CPBA (150 mg, 0.74 mmol, 85%) at 0 °C, and the resulting mixture was stirred at 0 °C for 10 min. The reaction mixture was used directly in the next step without further purification. MS (ESI, m / e) [M+H] + 357.1.

[0410] Step 5: (R)-8-chloro-7-fluoro-5-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-1,2,3,11,12,12a-hexahydro-10-oxa-3a,4,6,9-tetraazacyclopenta[4,5]cycloocta[1,2,3-de]naphthalene [ka]

[0411] To a stirred solution of (12aR)-8-chloro-7-fluoro-5-(methylsulfinyl)-1,2,3,11,12,12a-hexahydro-10-oxa-3a,4,6,9-tetraazacyclopenta[4,5]cycloocta[1,2,3-de]naphthalene (0.62 mmol) and ((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methanol (128 mg, 0.80 mmol) in THF (2.5 mL) was added LiHMDS (1.0 mL, 1.0 mmol, 1 M in THF) dropwise at 0 °C, and the resulting mixture was stirred at 0 °C for 20 min. The reaction was quenched with HO and concentrated under reduced pressure. The residue was purified by flash chromatography (CH2Cl2 / MeOH = 100:1 to 30:1) to give the product (223 mg). MS (ESI, m / e) [M+H] + 452.2.

[0412] Step 6: 3-chloro-5-((R)-7-fluoro-5-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-1,2,3,11,12,12a-hexahydro-10-oxa-3a,4,6,9-tetraazacyclopenta[4,5]cycloocta[1,2,3-de]naphthalen-8-yl)-4-(trifluoromethyl)aniline

[0413] (R)-8-chloro-7-fluoro-5-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-1,2,3,11,12,12a-hexahydro-10-oxa-3a,4,6,9-tetraazacyclopenta[4,5]cycloocta[1,2,3-de]naphthalene (25 mg, 0.055 mmol), 3-chloro-5 A mixture of -(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-4-(trifluoromethyl)aniline (40 mg, 0.11 mmol, 89%), Pd(dtbpf)Cl (7.0 mg, 0.011 mmol), NaHCO (14 mg, 0.17 mmol), dioxane (5.0 mL), and HO (1.0 mL) was stirred at 90 °C for 2 h. The reaction mixture was concentrated and purified by flash chromatography (CHCl / MeOH = 100:1 to 5:1) and then further purified by preparative HPLC to give the title product (1.2 mg). 1 H NMR (500MHz, CD3OD) δ 6.93- 6.81 (m, 1H),6.60 - 6.36 (m,1H), 5.50 - 5.27(m, 1H), 4.65 -4.50 (m, 1H), 4.50- 4.24 (m, 4H),4.09 - 3.95 (m,1H), 3.85 - 3.70(m, 1H), 3.19 -3.05 (m, 1H), 2.47- 1.82 (m, 12H). MS(ESI, m / e) [M+H] + 611.3.

[0414] Example 12: 3-((R)-7-fluoro-5-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-1,2,3,11,12,12a-hexahydro-10-oxa-3a,4,6,9-tetraazacyclopenta[4,5]cycloocta[1,2,3-de]naphthalen-8-yl)-5-methyl-4-(trifluoromethyl)aniline [ka]

[0415] (R)-8-chloro-7-fluoro-5-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-1,2,3,11,12,12a-hexahydro-10-oxa-3a,4,6,9-tetraazacyclopenta[4,5]cycloocta[1,2,3-de]naphthalene (25 mg, 0.055 mmol), 3-methyl-5 A mixture of -(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-4-(trifluoromethyl)aniline (34 mg, 0.11 mmol, 89%), Pd(dtbpf)Cl (7.0 mg, 0.011 mmol), NaHCO (14 mg, 0.17 mmol), dioxane (5.0 mL), and HO (1.0 mL) was stirred at 90 °C for 2 h. The reaction mixture was concentrated and purified by flash chromatography (CHCl / MeOH = 100:1 to 5:1), followed by further purification by preparative HPLC to give the title product (1.5 mg, 4%). 1 H NMR (500MHz, CDCl3) δ 6.70- 6.50 (m, 2H),5.44 - 5.25 (m,1H), 4.66 - 4.24(m, 5H), 4.10 -3.22 (m, 6H), 3.13- 3.01 (m, 1H),2.54 - 2.00 (m,14H). MS (ESI, m / e) [M+H] + 591.5.

[0416] Example 13: 3-chloro-5-((R)-7-fluoro-5-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-2,3,11,11a-tetrahydro-1H-10-oxa-3a,4,6,9-tetraazanaphtho[1,8-ef]azulen-8-yl)-4-(trifluoromethyl)aniline [ka]

[0417] Step 1: 4,5,7-trichloro-8-fluoro-2-(methylthio)pyrido[4,3-d]pyrimidine [ka]

[0418] To a solution of 5,7-dichloro-8-fluoro-2-(methylthio)pyrido[4,3-d]pyrimidin-4-ol (280 mg, 1.0 mmol) in ACN (10 mL) was added DIEA (194 mg, 1.5 mmol) and POCl (184 mg, 1.2 mmol), and the resulting mixture was stirred at 80° C. for 2 h. The reaction mixture was concentrated under reduced pressure and used directly in the next step without further purification.

[0419] Step 2: (R)-(1-(5,7-dichloro-8-fluoro-2-(methylthio)pyrido[4,3-d]pyrimidin-4-yl)pyrrolidin-2-yl)methanol [ka]

[0420] To a stirred solution of 4,5,7-trichloro-8-fluoro-2-(methylthio)pyrido[4,3-d]pyrimidine (1.0 mmol) in DCM (10 mL) was added DIEA (387 mg, 3.0 mmol) and (R)-pyrrolidin-2-ylmethanol (101 mg, 1.0 mmol) at 0 °C, and the resulting mixture was stirred at room temperature for 30 min. The reaction mixture was concentrated and purified by flash chromatography (PE / EtOAc = 4:1 to 1:2) to give the desired product (276 mg). MS (ESI, m / e) [M+H] + 363.1.

[0421] Step 3: (R)-8-chloro-7-fluoro-5-(methylthio)-2,3,11,11a-tetrahydro-1H-10-oxa-3a,4,6,9-tetraazanaphtho[1,8-ef]azulene [ka]

[0422] To a stirred solution of (R)-(1-(5,7-dichloro-8-fluoro-2-(methylthio)pyrido[4,3-d]pyrimidin-4-yl)pyrrolidin-2-yl)methanol (178 mg, 0.49 mmol) in THF (6 mL) was added NaH (40 mg, 0.98 mmol, 60%) at 0 °C, and the resulting mixture was stirred at room temperature for 2 h. The reaction was quenched with HO and then extracted with EtOAc. The organic layer was washed with brine, dried over anhydrous NaSO, and concentrated under reduced pressure. The residue was purified by flash chromatography (PE / EtOAc = 10:1 to 2:1) to give the product (154 mg). MS (ESI, m / e) [M+H] + 327.0.

[0423] Step 4: (11aR)-8-chloro-7-fluoro-5-(methylsulfinyl)-2,3,11,11a-tetrahydro-1H-10-oxa-3a,4,6,9-tetraazanaphtho[1,8-ef]azulene [ka]

[0424] To a stirred solution of (R)-8-chloro-7-fluoro-5-(methylthio)-2,3,11,11a-tetrahydro-1H-10-oxa-3a,4,6,9-tetraazanaphtho[1,8-ef]azulene (154 mg, 0.47 mmol) in DCM (8 mL) was added m-CPBA (115 mg, 0.56 mmol, 85%) at 0 °C, and the resulting mixture was stirred at 0 °C for 10 min. The reaction was quenched with aqueous NaSO and then extracted with DCM. The organic layer was washed with saturated aqueous NaHCO and brine, dried over anhydrous NaSO, and concentrated under reduced pressure. The residue was used directly in the next step without further purification. MS (ESI, m / e) [M+H] + 343.3.

[0425] Step 5: (R)-8-chloro-7-fluoro-5-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-2,3,11,11a-tetrahydro-1H-10-oxa-3a,4,6,9-tetraazanaphtho[1,8-ef]azulene [ka]

[0426] To a stirred solution of (11aR)-8-chloro-7-fluoro-5-(methylsulfinyl)-2,3,11,11a-tetrahydro-1H-10-oxa-3a,4,6,9-tetraazanaphtho[1,8-ef]azulene (0.47 mmol) and ((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methanol (149 mg, 0.94 mmol) in THF (8 mL) was added LiHMDS (0.94 mL, 0.94 mmol, 1 M in THF) dropwise at 0 °C, and the resulting mixture was stirred at 0 °C for 20 min. The reaction was quenched with saturated aqueous NH4Cl and then extracted with EtOAc. The organic layer was washed with brine, dried over anhydrous Na2SO4, and concentrated under reduced pressure. The residue was purified by flash chromatography (DCM / MeOH=100:1 to 30:1) to give the product (110 mg, 53%). MS (ESI, m / e) [M+H] + 438.5.

[0427] Step 6: 3-chloro-5-((R)-7-fluoro-5-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-2,3,11,11a-tetrahydro-1H-10-oxa-3a,4,6,9-tetraazanaphtho[1,8-ef]azulen-8-yl)-4-(trifluoromethyl)aniline

[0428] A mixture of (R)-8-chloro-7-fluoro-5-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-2,3,11,11a-tetrahydro-1H-10-oxa-3a,4,6,9-tetraazanaphtho[1,8-ef]azulene (30 mg, 0.07 mmol), 3-chloro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-4-(trifluoromethyl)aniline (50 mg, 0.14 mmol, 89%), Pd(dtbpf)Cl (9.0 mg, 0.014 mmol), NaHCO (18 mg, 0.21 mmol), dioxane (2.0 mL), and HO (0.4 mL) was stirred at 90 °C for 2 h. The reaction mixture was concentrated and purified by flash chromatography (DCM / MeOH=100:1 to 5:1) and then preparative HPLC to give the title product (12.6 mg). 1 H NMR (500MHz, CD3OD) δ 6.90- 6.83 (m, 1H),6.56 - 6.40 (m,1H), 5.43 - 5.21(m, 1H), 4.76 -4.66 (m, 2H), 4.41- 4.09 (m, 4H),4.03 - 3.85 (m,2H), 3.27 - 3.03(m, 2H), 2.43 -1.80 (m, 10H). MS (ESI, m / e) [M+H] + 597.4.

[0429] Example 14: 5-ethynyl-6-fluoro-4-((R)-7-fluoro-5-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-2,3,11,11a-tetrahydro-1H-10-oxa-3a,4,6,9-tetraazanaphtho[1,8-ef]azulen-8-yl)naphthalen-2-ol [ka]

[0430] Step 1: (R)-7-fluoro-8-(7-fluoro-3-(methoxymethoxy)-8-((triisopropylsilyl)ethynyl)naphthalen-1-yl)-5-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-2,3,11,11a-tetrahydro-1H-10-oxa-3a,4,6,9-tetraazanaphtho[1,8-ef]azulene [ka]

[0431] (R)-8-chloro-7-fluoro-5-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-2,3,11,11a-tetrahydro-1H-10-oxa-3a,4,6,9-tetraazanaphtho[1,8-ef]azulene (50 mg, 0.11 mmol), ((2-fluoro-6-(methoxymethoxy)-8-(4,4 A mixture of 5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)naphthalen-1-yl)ethynyl)triisopropylsilane (113 mg, 0.22 mmol), Pd(dtbpf)Cl (14 mg, 0.022 mmol), NaHCO (28 mg, 0.33 mmol), dioxane (3.0 mL), and HO (0.6 mL) was stirred at 90 °C for 2 h. The reaction mixture was concentrated and purified by flash chromatography (DCM / MeOH = 100:1 to 10:1) to give the title product (65 mg). MS (ESI, m / e) [M+H] + 788.8.

[0432] Step 2: (R)-8-(8-ethynyl-7-fluoro-3-(methoxymethoxy)naphthalen-1-yl)-7-fluoro-5-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-2,3,11,11a-tetrahydro-1H-10-oxa-3a,4,6,9-tetraazanaphtho[1,8-ef]azulene [ka]

[0433] To a stirred solution of (R)-7-fluoro-8-(7-fluoro-3-(methoxymethoxy)-8-((triisopropylsilyl)ethynyl)naphthalen-1-yl)-5-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-2,3,11,11a-tetrahydro-1H-10-oxa-3a,4,6,9-tetraazanaphtho[1,8-ef]azulene (65 mg, 0.08 mmol) in DMF (4 mL) was added CsF (122 mg, 0.8 mmol), and the resulting mixture was stirred at room temperature for 2 h. The reaction mixture was diluted with EtOAc and HO and then extracted with EtOAc. The organic layer was washed with brine, dried over anhydrous NaSO, and concentrated under reduced pressure. The crude product was used directly in the next step without further purification. MS (ESI, m / e) [M+H] + 632.5.

[0434] Step 3: 5-ethynyl-6-fluoro-4-((R)-7-fluoro-5-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-2,3,11,11a-tetrahydro-1H-10-oxa-3a,4,6,9-tetraazanaphtho[1,8-ef]azulen-8-yl)naphthalen-2-ol [ka]

[0435] To a stirred solution of (R)-8-(8-ethynyl-7-fluoro-3-(methoxymethoxy)naphthalen-1-yl)-7-fluoro-5-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-2,3,11,11a-tetrahydro-1H-10-oxa-3a,4,6,9-tetraazanaphtho[1,8-ef]azulene (0.08 mmol) in DCM (3.0 mL) was added 4 N HCl (in dioxane) (0.5 mL) at 0 °C, and the resulting mixture was stirred at room temperature for 1 h. The reaction mixture was concentrated and basified with DIEA, and the residue was purified by flash chromatography (DCM / MeOH = 100:1 to 10:1) followed by preparative HPLC to give the product (18.2 mg). 1 H NMR (500MHz, CD3OD) δ 7.93- 7.72 (m, 1H),7.41 - 7.12 (m,3H), 5.49 - 5.31(m, 1H), 4.79 -4.72 (m, 1H), 4.54- 3.88 (m, 6H),3.65 - 3.41 (m,5H), 3.23 - 3.13(m, 1H), 2.53 -1.78 (m, 10H). MS (ESI, m / e) [M+H] + 588.6.

[0436] Example 15: 3-chloro-5-((S)-1-fluoro-11-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-5,5a,6,7,8,9-hexahydro-4-oxa-3,9a,10,12-tetraazabenzo[4,5]cyclohepta[1,2,3-de]naphthalen-2-yl)-4-(trifluoromethyl)aniline [ka]

[0437] Step 1: (S)-7-chloro-8-fluoro-2-(methylthio)-5-(piperidin-2-ylmethoxy)pyrido[4,3-d]pyrimidin-4-ol [ka]

[0438] To a stirred solution of 5,7-dichloro-8-fluoro-2-(methylthio)pyrido[4,3-d]pyrimidin-4-ol (200 mg, 0.72 mmol) and (S)-piperidin-2-ylmethanol (82 mg, 0.72 mmol) in THF (6 mL) was added NaH (100 mg, 2.5 mmol, 60%) at 0° C., and the resulting mixture was stirred at room temperature for 2 hours. The reaction mixture was quenched with HO, and then the pH was adjusted to 6 with 1N aqueous HCl. The mixture was filtered, and the filter cake was used directly in the next step without further purification. MS (ESI, m / e) [M+H] + 359.1.

[0439] Step 2: (S)-2-chloro-1-fluoro-11-(methylthio)-5,5a,6,7,8,9-hexahydro-4-oxa-3,9a,10,12-tetraazabenzo[4,5]cyclohepta[1,2,3-de]naphthalene [ka]

[0440] To a stirred solution of (S)-7-chloro-8-fluoro-2-(methylthio)-5-(piperidin-2-ylmethoxy)pyrido[4,3-d]pyrimidin-4-ol (0.72 mmol) in MeCN (50 mL) was added DIEA (186 mg, 1.4 mmol) and HATU (410 mg, 1.1 mmol) at room temperature, and the resulting mixture was stirred at room temperature for 2 hours. The reaction mixture was concentrated and purified by flash chromatography (PE / EtOAc = 4:1 to 1:3) to give the desired product (184 mg). MS (ESI, m / e) [M+H] + 341.1.

[0441] Step 3: (5aS)-2-chloro-1-fluoro-11-(methylsulfinyl)-5,5a,6,7,8,9-hexahydro-4-oxa-3,9a,10,12-tetraazabenzo[4,5]cyclohepta[1,2,3-de]naphthalene [ka]

[0442] To a stirred solution of (S)-2-chloro-1-fluoro-11-(methylthio)-5,5a,6,7,8,9-hexahydro-4-oxa-3,9a,10,12-tetraazabenzo[4,5]cyclohepta[1,2,3-de]naphthalene (184 mg, 0.54 mmol) in DCM (15 mL) was added m-CPBA (120 mg, 0.59 mmol, 85%) at 0 °C, and the resulting mixture was stirred at 0 °C for 10 min. The reaction was quenched with aqueous NaSO and then extracted with DCM. The organic layer was washed with saturated aqueous NaHCO and brine, dried over anhydrous NaSO, and concentrated under reduced pressure. The residue was used directly in the next step without further purification.

[0443] Step 4: (S)-2-chloro-1-fluoro-11-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-5,5a,6,7,8,9-hexahydro-4-oxa-3,9a,10,12-tetraazabenzo[4,5]cyclohepta[1,2,3-de]naphthalene [ka]

[0444] To a stirred solution of (5aS)-2-chloro-1-fluoro-11-(methylsulfinyl)-5,5a,6,7,8,9-hexahydro-4-oxa-3,9a,10,12-tetraazabenzo[4,5]cyclohepta[1,2,3-de]naphthalene (0.54 mmol) and ((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methanol (172 mg, 1.1 mmol) in THF (15 mL) was added LiHMDS (1.1 mL, 1.1 mmol, 1 M in THF) dropwise at 0 °C, and the resulting mixture was stirred at 0 °C for 20 min. The reaction was quenched with saturated aqueous NH4Cl and then extracted with EtOAc. The organic layer was washed with brine, dried over anhydrous Na2SO4, and concentrated under reduced pressure. The residue was purified by flash chromatography (DCM / MeOH=100:1 to 30:1) to give the product (96 mg). MS (ESI, m / e) [M+H] + 452.3.

[0445] Step 5: 3-chloro-5-((S)-1-fluoro-11-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-5,5a,6,7,8,9-hexahydro-4-oxa-3,9a,10,12-tetraazabenzo[4,5]cyclohepta[1,2,3-de]naphthalen-2-yl)-4-(trifluoromethyl)aniline

[0446] (S)-2-chloro-1-fluoro-11-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-5,5a,6,7,8,9-hexahydro-4-oxa-3,9a,10,12-tetraazabenzo[4,5]cyclohepta[1,2,3-de]naphthalene (32 mg, 0.07 mmol), 3-chloro-5-( A mixture of 4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-4-(trifluoromethyl)aniline (45 mg, 0.14 mmol, 89%), Pd(dtbpf)Cl (9 mg, 0.014 mmol), NaHCO (18 mg, 0.21 mmol), dioxane (3.0 mL), and HO (0.6 mL) was stirred at 90° C. for 2 hours. The reaction mixture was concentrated and purified by flash chromatography (DCM / MeOH=100:1 to 5:1) followed by preparative HPLC to give the title product (10.6 mg). 1 H NMR (500MHz, CD3OD) δ 6.91- 6.84 (m, 1H),6.53 - 6.42 (m,1H), 5.49 - 5.22(m, 2H), 4.57 -4.42 (m, 2H), 4.41- 4.20 (m, 2H),4.02 - 3.85 (m,1H), 3.56 - 3.33(m, 3H), 3.15 -3.00 (m, 2H), 2.46- 1.51 (m, 12H). MS(ESI, m / e) [M+H] + 611.4.

[0447] Example 16: 5-ethynyl-6-fluoro-4-((S)-1-fluoro-11-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-5,5a,6,7,8,9-hexahydro-4-oxa-3,9a,10,12-tetraazabenzo[4,5]cyclohepta[1,2,3-de]naphthalen-2-yl)naphthalen-2-ol [ka]

[0448] Step 1: (S)-1-fluoro-2-(7-fluoro-3-(methoxymethoxy)-8-((triisopropylsilyl)ethynyl)naphthalen-1-yl)-11-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-5,5a,6,7,8,9-hexahydro-4-oxa-3,9a,10,12-tetraazabenzo[4,5]cyclohepta[1,2,3-de]naphthalene [ka]

[0449] (S)-2-chloro-1-fluoro-11-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-5,5a,6,7,8,9-hexahydro-4-oxa-3,9a,10,12-tetraazabenzo[4,5]cyclohepta[1,2,3-de]naphthalene (60 mg, 0.13 mmol), ((2-fluoro-6-(methoxymethoxy) A mixture of 4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)naphthalen-1-yl)ethynyl)triisopropylsilane (133 mg, 0.26 mmol), Pd(dtbpf)Cl (18 mg, 0.03 mmol), NaHCO (33 mg, 0.39 mmol), dioxane (5.0 mL), and HO (1.0 mL) was stirred at 90 °C for 2 h. The reaction mixture was concentrated and purified by flash chromatography (DCM / MeOH = 100:1 to 10:1) to give the title product (95 mg).

[0450] Step 2: (S)-2-(8-ethynyl-7-fluoro-3-(methoxymethoxy)naphthalen-1-yl)-1-fluoro-11-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-5,5a,6,7,8,9-hexahydro-4-oxa-3,9a,10,12-tetraazabenzo[4,5]cyclohepta[1,2,3-de]naphthalene [ka]

[0451] To a stirred solution of (S)-1-fluoro-2-(7-fluoro-3-(methoxymethoxy)-8-((triisopropylsilyl)ethynyl)naphthalen-1-yl)-11-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-5,5a,6,7,8,9-hexahydro-4-oxa-3,9a,10,12-tetraazabenzo[4,5]cyclohepta[1,2,3-de]naphthalene (95 mg, 0.11 mmol) in DMF (4 mL) was added CsF (152 mg, 1.1 mmol), and the resulting mixture was stirred at room temperature for 2 h. The reaction mixture was diluted with EtOAc and HO and then extracted with EtOAc. The organic layer was washed with brine, dried over anhydrous NaSO, and concentrated under reduced pressure. The crude product was used directly in the next step without further purification. MS (ESI, m / e) [M+H] + 646.1.

[0452] Step 3: 5-ethynyl-6-fluoro-4-((S)-1-fluoro-11-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-5,5a,6,7,8,9-hexahydro-4-oxa-3,9a,10,12-tetraazabenzo[4,5]cyclohepta[1,2,3-de]naphthalen-2-yl)naphthalen-2-ol

[0453] To a stirred solution of (S)-2-(8-ethynyl-7-fluoro-3-(methoxymethoxy)naphthalen-1-yl)-1-fluoro-11-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-5,5a,6,7,8,9-hexahydro-4-oxa-3,9a,10,12-tetraazabenzo[4,5]cyclohepta[1,2,3-de]naphthalene (0.11 mmol) in DCM (5.0 mL) was added 4N HCl (in dioxane) (1.0 mL) at 0° C., and the resulting mixture was stirred at room temperature for 1 h. The reaction mixture was concentrated and basified with DIEA, and the residue was purified by flash chromatography (DCM / MeOH=100:1 to 10:1) followed by preparative HPLC to give the product (38.6 mg). 1 H NMR (500MHz, CD3OD) δ 7.90- 7.77 (m, 1H),7.37 - 7.13 (m,3H), 5.48 - 5.27(m, 2H), 4.61 -4.47 (m, 2H), 4.46- 4.26 (m, 2H),4.05 - 3.89 (m,1H), 3.58 - 3.35(m, 4H), 3.20 -3.02 (m, 2H), 2.51- 1.55 (m, 12H). MS(ESI, m / e) [M+H] + 602.5.

[0454] Example 17: 3-chloro-5-(1,7-difluoro-5-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-2,3,11,11a-tetrahydro-1H-10-oxa-3a,4,6,9-tetraazanaphtho[1,8-ef]azulen-8-yl)-4-(trifluoromethyl)aniline [ka]

[0455] Step 1: 1-(5,7-dichloro-8-fluoro-2-(methylthio)pyrido[4,3-d]pyrimidin-4-yl)-2-(hydroxymethyl)pyrrolidin-3-ol [ka]

[0456] To a solution of 4,5,7-trichloro-8-fluoro-2-(methylthio)pyrido[4,3-d]pyrimidine (299 mg, 1.0 mmol) in DCM (30 mL) was added DIPEA (322 mg, 2.5 mmol) and 2-(hydroxymethyl)pyrrolidin-3-ol (118 mg, 1.17 mmol) at 0° C. The resulting mixture was stirred from 0° C. to room temperature for 1 hour. Upon completion of stirring, the reaction mixture was concentrated to give a residue. The residue was purified by flash silica column (PE: EtOAc = 1:1) to give the title product (156 mg). MS (ESI, m / e) [M+H] + 379.0.

[0457] Step 2: 8-chloro-7-fluoro-5-(methylthio)-2,3,11,11a-tetrahydro-1H-10-oxa-3a,4,6,9-tetraazanaphtho[1,8-ef]azulen-1-ol [ka]

[0458] To a solution of 1-(5,7-dichloro-8-fluoro-2-(methylthio)pyrido[4,3-d]pyrimidin-4-yl)-2-(hydroxymethyl)pyrrolidin-3-ol (156 mg, 0.42 mmol) in THF (15 mL) was added NaH (34 mg, 0.85 mmol) at room temperature, and the mixture was stirred at room temperature for 2 hours. After stirring was complete, the mixture was evaporated. The residue was purified by silica chromatography column (PE: EtOAc = 1:1) to give the title product (227 mg, crude). MS (ESI, m / e) [M+H] + 343.0.

[0459] Step 3: 8-chloro-1,7-difluoro-5-(methylthio)-2,3,11,11a-tetrahydro-1H-10-oxa-3a,4,6,9-tetraazanaphtho[1,8-ef]azulene [ka]

[0460] To a solution of 8-chloro-7-fluoro-5-(methylthio)-2,3,11,11a-tetrahydro-1H-10-oxa-3a,4,6,9-tetraazanaphtho[1,8-ef]azulen-1-ol (227 mg, 0.66 mmol) in DCM (10 mL), DAST (159 mg, 0.99 mmol) was added at room temperature, and the mixture was stirred at room temperature for 1 hour. After stirring was complete, the reaction mixture was evaporated. The residue was purified by silica chromatography column (DCM:MeOH=20:1) to give the title product (46 mg). MS (ESI, m / e) [M+H] + 345.0.

[0461] Step 4: 8-chloro-1,7-difluoro-5-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-2,3,11,11a-tetrahydro-1H-10-oxa-3a,4,6,9-tetraazanaphtho[1,8-ef]azulene [ka]

[0462] To a solution of 8-chloro-1,7-difluoro-5-(methylthio)-2,3,11,11a-tetrahydro-1H-10-oxa-3a,4,6,9-tetraazanaphtho[1,8-ef]azulene (46 mg, 0.16 mmol) in DCM (10 mL) was added m-CPBA (28 mg, 0.16 mmol) at room temperature, and the mixture was stirred at room temperature for 1 hour as mixture 1. Meanwhile, to a solution of ((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methanol (78 mg, 0.49 mmol) in 15 mL of THF, LiHMDS (1N in THF, 0.3 mL, 0.3 mmol) was added at room temperature, and the mixture was stirred at room temperature for 1 hour as mixture 2. Then, mixture 2 was added to mixture 1 at room temperature, and the mixture was stirred at room temperature for 2 hours. After stirring was completed, the reaction mixture was evaporated. The residue was purified by silica chromatography column (DCM:MeOH=10:1) to give the title product (23 mg, crude). MS (ESI, m / e) [M+H] + 456.1.

[0463] Step 5: 3-chloro-5-(1,7-difluoro-5-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-2,3,11,11a-tetrahydro-1H-10-oxa-3a,4,6,9-tetraazanaphtho[1,8-ef]azulen-8-yl)-4-(trifluoromethyl)aniline

[0464] 3-chloro-5-(1,7-difluoro-5-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-2,3,11,11a-tetrahydro-1H-10-oxa-3a,4,6,9-tetraazanaphtho[1,8-ef]azulen-8-yl)-4-(trifluoromethyl)- A mixture of 3-chloro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-4-(trifluoromethyl)aniline (23 mg, 0.05 mmol), 3-chloro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-4-(trifluoromethyl)aniline (64 mg, 0.2 mmol), NaHCO (13 mg, 0.15 mmol), and Pd(dtbpf)Cl (3.3 mg, 0.005 mmol) was stirred at 95 °C for 3 hours. The resulting mixture was concentrated and purified by column chromatography (DCM / MeOH = 10 / 1) to give a residue, which was further purified by preparative HPLC to give the title product (1.6 mg). 1 H NMR (500MHz, CD3OD) δ 6.91- 6.85 (m, 1H),6.55 - 6.41 (m,1H), 5.45 - 5.06(m, 2H), 4.91 -4.88 (m, 1H), 4.42- 3.98 (m, 6H),3.49 - 3.33 (m,3H), 3.13 - 3.08(m, 1H), 2.50 -1.89 (m, 8H). MS (ESI, m / e) [M+H] + 615.1.

[0465] Example 18: 5-ethynyl-6-fluoro-4-((R)-6-fluoro-4-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-1,2,10,10a-tetrahydro-9-oxa-2a,3,5,8-tetraazacyclobuta[4,5]cyclohepta[1,2,3-de]naphthalen-7-yl)naphthalen-2-ol [ka]

[0466] Step 1: 4,5,7-trichloro-8-fluoro-2-(methylthio)pyrido[4,3-d]pyrimidine [ka]

[0467] To a solution of 5,7-dichloro-8-fluoro-2-(methylthio)pyrido[4,3-d]pyrimidin-4-ol (280 mg, 1.0 mmol) in ACN (10 mL) was added DIEA (194 mg, 1.5 mmol) and POCl (184 mg, 1.2 mmol), and the resulting mixture was stirred at 80° C. for 2 h. The reaction mixture was concentrated under reduced pressure and used directly in the next step without further purification.

[0468] Step 2: (R)-(1-(5,7-dichloro-8-fluoro-2-(methylthio)pyrido[4,3-d]pyrimidin-4-yl)azetidin-2-yl)methanol [ka]

[0469] To a stirred solution of 4,5,7-trichloro-8-fluoro-2-(methylthio)pyrido[4,3-d]pyrimidine (200 mg crude product) in DCM (5.0 mL) was added DIEA (129 mg, 1.0 mmol) and (R)-azetidin-2-ylmethanol (44 mg, 0.5 mmol) at 0 °C, and the resulting mixture was stirred at room temperature for 30 min. The reaction mixture was concentrated and purified by flash chromatography (PE / EtOAc = 4:1 to 1:2) to give the desired product (90 mg). MS (ESI, m / e) [M+H] + 349.2.

[0470] Step 3: (R)-7-chloro-6-fluoro-4-(methylthio)-1,2,10,10a-tetrahydro-9-oxa-2a,3,5,8-tetraazacyclobuta[4,5]cyclohepta[1,2,3-de]naphthalene [ka]

[0471] To a stirred solution of (R)-(1-(5,7-dichloro-8-fluoro-2-(methylthio)pyrido[4,3-d]pyrimidin-4-yl)azetidin-2-yl)methanol (90 mg, 0.26 mmol) in THF (3.0 mL) was added NaH (31 mg, 0.78 mmol, 60%) at 0° C., and the resulting mixture was stirred at room temperature for 2 h. The reaction was quenched with HO and then extracted with EtOAc. The organic layer was washed with brine, dried over anhydrous NaSO, and concentrated under reduced pressure. The residue was used directly in the next step without further purification. MS (ESI, m / e) [M+H] + 313.1.

[0472] Step 4: (10aR)-7-chloro-6-fluoro-4-(methylsulfinyl)-1,2,10,10a-tetrahydro-9-oxa-2a,3,5,8-tetraazacyclobuta[4,5]cyclohepta[1,2,3-de]naphthalene [ka]

[0473] To a stirred solution of (R)-7-chloro-6-fluoro-4-(methylthio)-1,2,10,10a-tetrahydro-9-oxa-2a,3,5,8-tetraazacyclobuta[4,5]cyclohepta[1,2,3-de]naphthalene (0.26 mmol) in DCM (5.0 mL) was added m-CPBA (63 mg, 0.31 mmol, 85%) at 0 °C, and the resulting mixture was stirred at 0 °C for 10 min. The reaction was quenched with aqueous NaSO and then extracted with DCM. The organic layer was washed with saturated aqueous NaHCO and brine, dried over anhydrous NaSO, and concentrated under reduced pressure. The residue was used directly in the next step without further purification.

[0474] Step 5: (R)-7-chloro-6-fluoro-4-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-1,2,10,10a-tetrahydro-9-oxa-2a,3,5,8-tetraazacyclobuta[4,5]cyclohepta[1,2,3-de]naphthalene [ka]

[0475] To a stirred solution of (10aR)-7-chloro-6-fluoro-4-(methylsulfinyl)-1,2,10,10a-tetrahydro-9-oxa-2a,3,5,8-tetraazacyclobuta[4,5]cyclohepta[1,2,3-de]naphthalene (0.26 mmol) and ((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methanol (83 mg, 0.52 mmol) in THF (5.0 mL), LiHMDS (0.52 mL, 0.52 mmol, 1 M in THF) was added dropwise at 0 °C, and the resulting mixture was stirred at 0 °C for 20 min. The mixture was evaporated and purified by flash chromatography (DCM / MeOH = 100:1 to 30:1) to give the title product (56 mg). MS (ESI, m / e) [M+H] + 424.1.

[0476] Step 6: (R)-6-Fluoro-7-(7-fluoro-3-(methoxymethoxy)-8-((triisopropylsilyl)ethynyl)naphthalen-1-yl)-4-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-1,2,10,10a-tetrahydro-9-oxa-2a,3,5,8-tetraazacyclobuta[4,5]cyclohepta[1,2,3-de]naphthalene [ka]

[0477] (R)-7-chloro-6-fluoro-4-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-1,2,10,10a-tetrahydro-9-oxa-2a,3,5,8-tetraazacyclobuta[4,5]cyclohepta[1,2,3-de]naphthalene (28 mg, 0.07 mmol), ((2-fluoro-6-(methoxymethoxy) A mixture of 8-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)naphthalen-1-yl)ethynyl)triisopropylsilane (72 mg, 0.14 mmol), Pd(dtbpf)Cl (9 mg, 0.014 mmol), NaHCO (18 mg, 0.21 mmol), dioxane (3.0 mL), and HO (0.6 mL) was stirred at 90 °C for 2 h. The reaction mixture was concentrated and purified by flash chromatography (DCM / MeOH = 100:1 to 10:1) to give the title product (32 mg). MS (ESI, m / e) [M+H] + 774.2.

[0478] Step 7: (R)-7-(8-ethynyl-7-fluoro-3-(methoxymethoxy)naphthalen-1-yl)-6-fluoro-4-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-1,2,10,10a-tetrahydro-9-oxa-2a,3,5,8-tetraazacyclobuta[4,5]cyclohepta[1,2,3-de]naphthalene [ka]

[0479] To a stirred solution of (R)-6-fluoro-7-(7-fluoro-3-(methoxymethoxy)-8-((triisopropylsilyl)ethynyl)naphthalen-1-yl)-4-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-1,2,10,10a-tetrahydro-9-oxa-2a,3,5,8-tetraazacyclobuta[4,5]cyclohepta[1,2,3-de]naphthalene (32 mg, 0.043 mmol) in DMF (3.0 mL) was added CsF (65 mg, 0.43 mmol), and the resulting mixture was stirred at room temperature for 2 h. The reaction mixture was diluted with EtOAc and HO and then extracted with EtOAc. The organic layer was washed with brine, dried over anhydrous NaSO, and concentrated under reduced pressure. The crude product was used directly in the next step without further purification. MS (ESI, m / e) [M+H] + 618.3.

[0480] Step 8: 5-ethynyl-6-fluoro-4-((R)-6-fluoro-4-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-1,2,10,10a-tetrahydro-9-oxa-2a,3,5,8-tetraazacyclobuta[4,5]cyclohepta[1,2,3-de]naphthalen-7-yl)naphthalen-2-ol

[0481] To a stirred solution of (R)-7-(8-ethynyl-7-fluoro-3-(methoxymethoxy)naphthalen-1-yl)-6-fluoro-4-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-1,2,10,10a-tetrahydro-9-oxa-2a,3,5,8-tetraazacyclobuta[4,5]cyclohepta[1,2,3-de]naphthalene (0.043 mmol) in DCM (3.0 mL) was added 4 N HCl (in dioxane) (1.0 mL) at 0 °C, and the resulting mixture was stirred at room temperature for 1 h. The reaction mixture was concentrated and basified with DIEA, and the residue was purified by flash chromatography (DCM / MeOH = 100:1 to 10:1) followed by preparative HPLC to give the product (6.5 mg).1 H NMR (500MHz, CD3OD) δ 7.96- 7.73 (m, 1H),7.50 - 7.10 (m,3H), 5.45 - 5.26(m, 1H), 5.23 -5.06 (m, 1H), 4.74- 4.62 (m, 1H),4.57 - 4.26 (m,5H), 3.61 - 3.35(m, 4H), 3.19 -3.06 (m, 1H), 2.70- 1.85 (m, 8H). MS(ESI, m / e) [M+H] + 574.1.

[0482] Example 19: 4-((2R,11aS)-2,7-difluoro-5-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-2,3,11,11a-tetrahydro-1H-10-oxa-3a,4,6,9-tetraazanaphtho[1,8-ef]azulen-8-yl)-5-ethynyl-6-fluoronaphthalen-2-ol [ka]

[0483] Step 1: ((2S,4R)-1-(5,7-dichloro-8-fluoro-2-(methylthio)pyrido[4,3-d]pyrimidin-4-yl)-4-fluoropyrrolidin-2-yl)methanol [ka]

[0484] A solution of 4,5,7-trichloro-8-fluoro-2-(methylthio)pyrido[4,3-d]pyrimidine (200 mg) and ((2S,4R)-4-fluoropyrrolidin-2-yl)methanol hydrochloride (115 mg, 0.74 mmol) in DIEA (0.5 mL) and CHCN (10 mL) was stirred at room temperature for 2 hours. After stirring was complete, the mixture was concentrated under reduced pressure, and the crude product was purified by silica column (eluted with PE / EtOAc = 1 / 1) to give the title product (140 mg). MS (ESI, m / e) [M+H]+ 381.1

[0485] Step 2: (2R,11aS)-8-chloro-2,7-difluoro-5-(methylthio)-2,3,11,11a-tetrahydro-1H-10-oxa-3a,4,6,9-tetraazanaphtho[1,8-ef]azulene [ka]

[0486] To a solution of ((2S,4R)-1-(5,7-dichloro-8-fluoro-2-(methylthio)pyrido[4,3-d]pyrimidin-4-yl)-4-fluoropyrrolidin-2-yl)methanol (140 mg) in THF (10 mL) was added NaH (40 mg). The mixture was stirred at room temperature for 1 hour. Upon completion of stirring, the reaction was quenched with water. The resulting mixture was evaporated, and the crude product was purified on a silica column (eluted with PE / EtOAc = 1 / 9) to give the title product (120 mg). (ESI, m / e) [M+H] + 346.2.

[0487] Step 3: (2R,11aS)-8-chloro-2,7-difluoro-5-(methylsulfinyl)-2,3,11,11a-tetrahydro-1H-10-oxa-3a,4,6,9-tetraazanaphtho[1,8-ef]azulene [ka]

[0488] To a mixture of (2R,11aS)-8-chloro-2,7-difluoro-5-(methylthio)-2,3,11,11a-tetrahydro-1H-10-oxa-3a,4,6,9-tetraazanaphtho[1,8-ef]azulene (120 mg, 0.35 mmol) in DCM (20 mL) was added m-CPBA (60 mg, 0.35 mmol). The mixture was stirred at room temperature for 1 hour. Upon completion of stirring, the resulting mixture was used directly in the next step. MS (ESI, m / e) [M+H] +361.1.

[0489] Step 4: (2R,11aS)-8-chloro-2,7-difluoro-5-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-2,3,11,11a-tetrahydro-1H-10-oxa-3a,4,6,9-tetraazanaphtho[1,8-ef]azulene [ka]

[0490] To a mixture of ((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methanol (100 mg, 0.63 mmol) in THF (20 mL), LiHMDS (0.4 mL) was added, and the mixture was stirred at room temperature for 1 hour. Then, (2R,11aS)-8-chloro-2,7-difluoro-5-(methylsulfinyl)-2,3,11,11a-tetrahydro-1H-10-oxa-3a,4,6,9-tetraazanaphtho[1,8-ef]azulene (20 mL of the mixture from the previous step) was added. After completion, the resulting mixture was concentrated. The crude product was then further purified by silica column (eluted with DCM / MeOH=9 / 1) to give the title product (84 mg). MS (ESI, m / e) [M+H] + 456.1.

[0491] Step 5: (2R,11aS)-2,7-difluoro-8-(7-fluoro-3-(methoxymethoxy)-8-((triisopropylsilyl)ethynyl)naphthalen-1-yl)-5-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-2,3,11,11a-tetrahydro-1H-10-oxa-3a,4,6,9-tetraazanaphtho[1,8-ef]azulene [ka]

[0492] (2R,11aS)-8-chloro-2,7-difluoro-5-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-2,3,11,11a-tetrahydro-1H-10-oxa-3a,4,6,9-tetraazanaphtho[1,8-ef]azulene (40 mg, A mixture of (2-fluoro-6-(methoxymethoxy)-8-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)naphthalen-1-yl)ethynyl)triisopropylsilane (50 mg, 0.097 mmol), Pd(dtbpf)Cl (20 mg), KPO (30 mg), and ((2-fluoro-6-(methoxymethoxy)-8-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)naphthalen-1-yl)ethynyl)triisopropylsilane (50 mg, 0.097 mmol) was stirred at 100 °C for 6 h. After stirring was complete, the solvent was evaporated and the crude product was purified on a silica column (eluted with DCM / MeOH = 5 / 1) to give the title product (10 mg). MS (ESI, m / e) [M+H] + 806.1

[0493] Step 6: (2R,11aS)-8-(8-ethynyl-7-fluoro-3-(methoxymethoxy)naphthalen-1-yl)-2,7-difluoro-5-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-2,3,11,11a-tetrahydro-1H-10-oxa-3a,4,6,9-tetraazanaphtho[1,8-ef]azulene [ka]

[0494] To a solution of (2R,11aS)-2,7-difluoro-8-(7-fluoro-3-(methoxymethoxy)-8-((triisopropylsilyl)ethynyl)naphthalen-1-yl)-5-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-2,3,11,11a-tetrahydro-1H-10-oxa-3a,4,6,9-tetraazanaphtho[1,8-ef]azulene (10 mg, 0.012 mmol) in DMF (5 mL) was added CsF (20 mg). The mixture was stirred at room temperature for 3 hours. Upon completion of stirring, the reaction was poured into water and extracted with DCM. The organic layer was concentrated and used directly in the next step. MS (ESI, m / e) [M+H] + 832.4.

[0495] Step 7: 4-((2R,11aS)-2,7-difluoro-5-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-2,3,11,11a-tetrahydro-1H-10-oxa-3a,4,6,9-tetraazanaphtho[1,8-ef]azulen-8-yl)-5-ethynyl-6-fluoronaphthalen-2-ol

[0496] A mixture of (2R,11aS)-8-(8-ethynyl-7-fluoro-3-(methoxymethoxy)naphthalen-1-yl)-2,7-difluoro-5-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-2,3,11,11a-tetrahydro-1H-10-oxa-3a,4,6,9-tetraazanaphtho[1,8-ef]azulene (10 mg of crude product) in dioxane / HCl (4 M) (2 mL) was stirred at room temperature for 2 hours. After stirring was complete, the resulting mixture was evaporated, and the residue was adjusted to pH=9 with DIPEA. The residue was then further purified by preparative HPLC to give the title product (1.5 mg). 1H NMR (500MHz, DMSO-d6) δ 10.14(s, 1H), 8.00- 7.91 (m, 1H), 7.48 - 7.41(m, 1H), 7.37(s, 1H), 7.20- 7.11 (m, 1H), 5.54 - 5.19(m, 2H), 4.78- 4.68 (m, 1H), 4.30 - 3.95(m, 7H), 3.31- 3.04 (m, 3H), 2.29 - 1.64(m, 7H). MS (ESI, m / e) [M+H] + 606.3.

[0497] Example 20: 5-ethynyl-6-fluoro-4-((S)-6-fluoro-4-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-1,2,10,10a-tetrahydro-9-oxa-2a,3,5,8-tetraazacyclobuta[4,5]cyclohepta[1,2,3-de]naphthalen-7-yl)naphthalen-2-ol [ka]

[0498] Step 1: 4,5,7-trichloro-8-fluoro-2-(methylthio)pyrido[4,3-d]pyrimidine [ka]

[0499] To a solution of 5,7-dichloro-8-fluoro-2-(methylthio)pyrido[4,3-d]pyrimidin-4-ol (200 mg, 0.71 mmol) in MeCN (10 mL) was added DIEA (120 mg, 0.92 mmol) and POCl (130 mg, 0.85 mmol), and the resulting mixture was stirred at 80° C. for 2 h. The reaction mixture was concentrated under reduced pressure and used directly in the next step without further purification.

[0500] Step 2: (S)-(1-(5,7-dichloro-8-fluoro-2-(methylthio)pyrido[4,3-d]pyrimidin-4-yl)azetidin-2-yl)methanol [ka]

[0501] To a stirred solution of 4,5,7-trichloro-8-fluoro-2-(methylthio)pyrido[4,3-d]pyrimidine (0.71 mmol) in DCM (10 mL) was added DIEA (271 mg, 2.1 mmol) and (S)-azetidin-2-ylmethanol (62 mg, 0.71 mmol) at 0 °C, and the resulting mixture was stirred at room temperature for 30 min. The reaction mixture was concentrated and purified by flash chromatography (PE / EtOAc = 4:1 to 1:2) to give the desired product (135 mg). MS (ESI, m / e) [M+H] + 349.0.

[0502] Step 3: (S)-7-chloro-6-fluoro-4-(methylthio)-1,2,10,10a-tetrahydro-9-oxa-2a,3,5,8-tetraazacyclobuta[4,5]cyclohepta[1,2,3-de]naphthalene [ka]

[0503] To a stirred solution of (S)-(1-(5,7-dichloro-8-fluoro-2-(methylthio)pyrido[4,3-d]pyrimidin-4-yl)azetidin-2-yl)methanol (135 mg, 0.39 mmol) in THF (6.0 mL) was added NaH (48 mg, 1.2 mmol, 60%) at 0° C., and the resulting mixture was stirred at room temperature for 2 h. The reaction was quenched with HO and then extracted with EtOAc. The organic layer was washed with brine, dried over anhydrous NaSO, and concentrated under reduced pressure. The residue was used directly in the next step without further purification. MS (ESI, m / e) [M+H] + 313.1.

[0504] Step 4: (10aS)-7-chloro-6-fluoro-4-(methylsulfinyl)-1,2,10,10a-tetrahydro-9-oxa-2a,3,5,8-tetraazacyclobuta[4,5]cyclohepta[1,2,3-de]naphthalene [ka]

[0505] To a stirred solution of (S)-7-chloro-6-fluoro-4-(methylthio)-1,2,10,10a-tetrahydro-9-oxa-2a,3,5,8-tetraazacyclobuta[4,5]cyclohepta[1,2,3-de]naphthalene (0.39 mmol) in DCM (8.0 mL) was added m-CPBA (83 mg, 0.48 mmol, 85%) at 0 °C, and the resulting mixture was stirred at 0 °C for 10 min. The reaction was quenched with aqueous NaSO and then extracted with DCM. The organic layer was washed with saturated aqueous NaHCO and brine, dried over anhydrous NaSO, and concentrated under reduced pressure. The residue was used directly in the next step without further purification.

[0506] Step 5: (S)-7-chloro-6-fluoro-4-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-1,2,10,10a-tetrahydro-9-oxa-2a,3,5,8-tetraazacyclobuta[4,5]cyclohepta[1,2,3-de]naphthalene [ka]

[0507] To a stirred solution of (10aS)-7-chloro-6-fluoro-4-(methylsulfinyl)-1,2,10,10a-tetrahydro-9-oxa-2a,3,5,8-tetraazacyclobuta[4,5]cyclohepta[1,2,3-de]naphthalene (0.39 mmol) and ((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methanol (127 mg, 0.80 mmol) in THF (8.0 mL), LiHMDS (0.80 mL, 0.80 mmol, 1 M in THF) was added dropwise at 0 °C, and the resulting mixture was stirred at 0 °C for 20 min. The residue was purified by flash chromatography (DCM / MeOH = 100:1 to 30:1) to give the title product (56 mg). MS (ESI, m / e) [M+H] + 424.2.

[0508] Step 6: (S)-6-Fluoro-7-(7-fluoro-3-(methoxymethoxy)-8-((triisopropylsilyl)ethynyl)naphthalen-1-yl)-4-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-1,2,10,10a-tetrahydro-9-oxa-2a,3,5,8-tetraazacyclobuta[4,5]cyclohepta[1,2,3-de]naphthalene [ka]

[0509] (S)-7-chloro-6-fluoro-4-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-1,2,10,10a-tetrahydro-9-oxa-2a,3,5,8-tetraazacyclobuta[4,5]cyclohepta[1,2,3-de]naphthalene (28 mg, 0.07 mmol), ((2-fluoro-6-(methoxymethoxy) A mixture of 8-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)naphthalen-1-yl)ethynyl)triisopropylsilane (72 mg, 0.14 mmol), Pd(dtbpf)Cl (9 mg, 0.014 mmol), NaHCO (18 mg, 0.21 mmol), dioxane (3.0 mL), and HO (0.6 mL) was stirred at 90 °C for 2 h. The reaction mixture was concentrated and purified by flash chromatography (DCM / MeOH = 100:1 to 10:1) to give the title product (12 mg). MS (ESI, m / e) [M+H] + 774.6.

[0510] Step 7: (S)-7-(8-ethynyl-7-fluoro-3-(methoxymethoxy)naphthalen-1-yl)-6-fluoro-4-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-1,2,10,10a-tetrahydro-9-oxa-2a,3,5,8-tetraazacyclobuta[4,5]cyclohepta[1,2,3-de]naphthalene [ka]

[0511] To a stirred solution of (S)-6-fluoro-7-(7-fluoro-3-(methoxymethoxy)-8-((triisopropylsilyl)ethynyl)naphthalen-1-yl)-4-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-1,2,10,10a-tetrahydro-9-oxa-2a,3,5,8-tetraazacyclobuta[4,5]cyclohepta[1,2,3-de]naphthalene (12 mg, 0.016 mmol) in DMF (3.0 mL) was added CsF (24 mg, 0.16 mmol), and the resulting mixture was stirred at room temperature for 2 h. The reaction mixture was diluted with EtOAc and HO and then extracted with EtOAc. The organic layer was washed with brine, dried over anhydrous NaSO, and concentrated under reduced pressure. The crude product was used directly in the next step without further purification. MS (ESI, m / e) [M+H] + 618.0.

[0512] Step 8: 5-ethynyl-6-fluoro-4-((S)-6-fluoro-4-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-1,2,10,10a-tetrahydro-9-oxa-2a,3,5,8-tetraazacyclobuta[4,5]cyclohepta[1,2,3-de]naphthalen-7-yl)naphthalen-2-ol

[0513] To a stirred solution of (S)-7-(8-ethynyl-7-fluoro-3-(methoxymethoxy)naphthalen-1-yl)-6-fluoro-4-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-1,2,10,10a-tetrahydro-9-oxa-2a,3,5,8-tetraazacyclobuta[4,5]cyclohepta[1,2,3-de]naphthalene (0.016 mmol) in DCM (2.0 mL) was added 4 N HCl (in dioxane) (0.4 mL) at 0 °C, and the resulting mixture was stirred at room temperature for 1 h. The reaction mixture was concentrated and basified with DIEA, and the residue was purified by flash chromatography (DCM / MeOH = 100:1 to 10:1) followed by preparative HPLC to give the product (0.92 mg).1 H NMR (500MHz, CD3OD) δ 7.92- 7.73 (m, 1H),7.37 - 7.11 (m,3H), 5.44 - 5.26(m, 1H), 5.20 -5.03 (m, 1H), 4.72- 4.25 (m, 6H),3.49 - 3.34 (m,4H), 3.19 - 3.05(m, 1H), 2.69 -1.85 (m, 8H). MS (ESI, m / e) [M+H] + 574.4.

[0514] Example 21: 5-ethynyl-6-fluoro-4-((S)-6-fluoro-4-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-1,10,11,11a-tetrahydro-2H-9-oxa-2a,3,5,8-tetraazacyclobuta[4,5]cycloocta[1,2,3-de]naphthalen-7-yl)naphthalen-2-ol [ka]

[0515] Step 1: 4,5,7-trichloro-8-fluoro-2-(methylthio)pyrido[4,3-d]pyrimidine [ka]

[0516] To a solution of 5,7-dichloro-8-fluoro-2-(methylthio)pyrido[4,3-d]pyrimidin-4-ol (200 mg, 0.71 mmol) in MeCN (10 mL) was added DIEA (120 mg, 0.92 mmol) and POCl (130 mg, 0.85 mmol), and the resulting mixture was stirred at 80° C. for 2 h. The reaction mixture was concentrated under reduced pressure and used directly in the next step without further purification.

[0517] Step 2: (S)-2-(1-(5,7-dichloro-8-fluoro-2-(methylthio)pyrido[4,3-d]pyrimidin-4-yl)azetidin-2-yl)ethan-1-ol [ka]

[0518] To a stirred solution of 4,5,7-trichloro-8-fluoro-2-(methylthio)pyrido[4,3-d]pyrimidine (0.71 mmol) in DCM (10 mL) was added DIEA (271 mg, 2.1 mmol) and (S)-2-(azetidin-2-yl)ethan-1-ol (72 mg, 0.71 mmol) at 0 °C, and the resulting mixture was stirred at 0 °C for 30 min. The reaction mixture was concentrated and purified by flash chromatography (PE / EtOAc = 4:1 to 1:2) to give the desired product (216 mg). MS (ESI, m / e) [M+H] + 363.2.

[0519] Step 3: (S)-7-chloro-6-fluoro-4-(methylthio)-1,10,11,11a-tetrahydro-2H-9-oxa-2a,3,5,8-tetraazacyclobuta[4,5]cycloocta[1,2,3-de]naphthalene [ka]

[0520] To a stirred solution of (S)-2-(1-(5,7-dichloro-8-fluoro-2-(methylthio)pyrido[4,3-d]pyrimidin-4-yl)azetidin-2-yl)ethan-1-ol (216 mg, 0.60 mmol) in THF (6.0 mL) was added NaH (72 mg, 1.8 mmol, 60%) at 0° C., and the resulting mixture was stirred at room temperature for 2 h. The reaction was quenched with HO and then extracted with EtOAc. The organic layer was washed with brine, dried over anhydrous NaSO, and concentrated under reduced pressure. The residue was used directly in the next step without further purification. MS (ESI, m / e) [M+H] + 327.1.

[0521] Step 4: (11aS)-7-chloro-6-fluoro-4-(methylsulfinyl)-1,10,11,11a-tetrahydro-2H-9-oxa-2a,3,5,8-tetraazacyclobuta[4,5]cycloocta[1,2,3-de]naphthalene [ka]

[0522] To a stirred solution of (S)-7-chloro-6-fluoro-4-(methylthio)-1,10,11,11a-tetrahydro-2H-9-oxa-2a,3,5,8-tetraazacyclobuta[4,5]cycloocta[1,2,3-de]naphthalene (0.60 mmol) in DCM (8.0 mL) was added m-CPBA (146 mg, 0.72 mmol, 85%) at 0 °C, and the resulting mixture was stirred at 0 °C for 10 min. The reaction was quenched with aqueous NaSO and then extracted with DCM. The organic layer was washed with saturated aqueous NaHCO and brine, dried over anhydrous NaSO, and concentrated under reduced pressure. The residue was used directly in the next step without further purification.

[0523] Step 5: (S)-7-chloro-6-fluoro-4-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-1,10,11,11a-tetrahydro-2H-9-oxa-2a,3,5,8-tetraazacyclobuta[4,5]cycloocta[1,2,3-de]naphthalene [ka]

[0524] To a stirred solution of (11aS)-7-chloro-6-fluoro-4-(methylsulfinyl)-1,10,11,11a-tetrahydro-2H-9-oxa-2a,3,5,8-tetraazacyclobuta[4,5]cycloocta[1,2,3-de]naphthalene (0.60 mmol) and ((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methanol (190 mg, 1.2 mmol) in THF (8.0 mL) was added LiHMDS (1.2 mL, 1.2 mmol, 1 M in THF) dropwise at 0 °C, and the resulting mixture was stirred at 0 °C for 20 min. The residue was purified by flash chromatography (DCM / MeOH = 100:1 to 30:1) to give the title product (64 mg). MS (ESI, m / e) [M+H] + 438.3.

[0525] Step 6: (S)-6-Fluoro-7-(7-fluoro-3-(methoxymethoxy)-8-((triisopropylsilyl)ethynyl)naphthalen-1-yl)-4-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-1,10,11,11a-tetrahydro-2H-9-oxa-2a,3,5,8-tetraazacyclobuta[4,5]cycloocta[1,2,3-de]naphthalene [ka]

[0526] (S)-7-chloro-6-fluoro-4-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-1,10,11,11a-tetrahydro-2H-9-oxa-2a,3,5,8-tetraazacyclobuta[4,5]cycloocta[1,2,3-de]naphthalene (32 mg, 0.073 mmol), ((2-fluoro-6-(methoxymeth) A mixture of (4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)naphthalen-1-yl)ethynyl)triisopropylsilane (77 mg, 0.15 mmol), Pd(dtbpf)Cl (10 mg, 0.02 mmol), NaHCO (19 mg, 0.22 mmol), dioxane (3.0 mL), and HO (0.6 mL) was stirred at 90 °C for 2 h. The reaction mixture was concentrated and purified by flash chromatography (DCM / MeOH = 100:1 to 10:1) to give the title product (32 mg). MS (ESI, m / e) [M+H] + 788.1.

[0527] Step 7: (S)-7-(8-ethynyl-7-fluoro-3-(methoxymethoxy)naphthalen-1-yl)-6-fluoro-4-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-1,10,11,11a-tetrahydro-2H-9-oxa-2a,3,5,8-tetraazacyclobuta[4,5]cycloocta[1,2,3-de]naphthalene [ka]

[0528] To a stirred solution of (S)-6-fluoro-7-(7-fluoro-3-(methoxymethoxy)-8-((triisopropylsilyl)ethynyl)naphthalen-1-yl)-4-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-1,10,11,11a-tetrahydro-2H-9-oxa-2a,3,5,8-tetraazacyclobuta[4,5]cycloocta[1,2,3-de]naphthalene (32 mg, 0.04 mmol) in DMF (3.0 mL) was added CsF (60 mg, 0.40 mmol), and the resulting mixture was stirred at room temperature for 2 h. The reaction mixture was diluted with EtOAc and HO and then extracted with EtOAc. The organic layer was washed with brine, dried over anhydrous NaSO, and concentrated under reduced pressure. The crude product was used directly in the next step without further purification. MS (ESI, m / e) [M+H] + 632.4.

[0529] Step 8: 5-ethynyl-6-fluoro-4-((S)-6-fluoro-4-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-1,10,11,11a-tetrahydro-2H-9-oxa-2a,3,5,8-tetraazacyclobuta[4,5]cycloocta[1,2,3-de]naphthalen-7-yl)naphthalen-2-ol

[0530] To a stirred solution of (S)-7-(8-ethynyl-7-fluoro-3-(methoxymethoxy)naphthalen-1-yl)-6-fluoro-4-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-1,10,11,11a-tetrahydro-2H-9-oxa-2a,3,5,8-tetraazacyclobuta[4,5]cycloocta[1,2,3-de]naphthalene (0.04 mmol) in DCM (3.0 mL) was added 4 N HCl (in dioxane) (0.4 mL) at 0 °C, and the resulting mixture was stirred at room temperature for 1 h. The reaction mixture was concentrated and basified with DIEA, and the residue was purified by flash chromatography (DCM / MeOH = 100:1 to 10:1) followed by preparative HPLC to give the product (6.5 mg). 1 H NMR (500MHz, CD3OD) 7.91 -7.75 (m, 1H), 7.39- 7.14 (m, 3H),5.45 - 5.27 (m,1H), 4.54 - 4.24(m, 6H), 3.66 -3.33 (m, 4H), 3.19- 3.04 (m, 1H),2.73 - 1.85 (m,10H). MS (ESI, m / e) [M+H] + 588.3.

[0531] Example 22: 3-chloro-5-((S)-6-fluoro-4-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-1,10,11,11a-tetrahydro-2H-9-oxa-2a,3,5,8-tetraazacyclobuta[4,5]cycloocta[1,2,3-de]naphthalen-7-yl)-4-(trifluoromethyl)aniline [ka]

[0532] (S)-7-chloro-6-fluoro-4-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-1,10,11,11a-tetrahydro-2H-9-oxa-2a,3,5,8-tetraazacyclobuta[4,5]cycloocta[1,2,3-de]naphthalene (32 mg, 0.073 mmol), 3-chloro- A mixture of 5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-4-(trifluoromethyl)aniline (48 mg, 0.15 mmol), Pd(dtbpf)Cl (10 mg, 0.02 mmol), NaHCO (19 mg, 0.22 mmol), dioxane (3.0 mL), and HO (0.6 mL) was stirred at 90° C. for 2 hours. The reaction mixture was concentrated and purified by flash chromatography (DCM / MeOH = 100:1 to 20:1) to give the title product (10.8 mg). 1 H NMR (500MHz, CD3OD) δ 6.92- 6.85 (m, 1H),6.58 - 6.39 (m,1H), 5.48 - 5.22(m, 1H), 4.56 -4.23 (m, 6H), 3.50- 3.32 (m, 3H),3.15 - 3.01 (m,1H), 2.75 - 1.84(m, 10H). MS (ESI, m / e) [M+H] + 597.4.

[0533] Example 23: 3-chloro-5-((R)-6-fluoro-4-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-1,10,11,11a-tetrahydro-2H-9-oxa-2a,3,5,8-tetraazacyclobuta[4,5]cycloocta[1,2,3-de]naphthalen-7-yl)-4-(trifluoromethyl)aniline

[0534] [ka]

[0535] Step 1: (R)-tert-butyl 2-(2-hydroxyethyl)azetidine-1-carboxylate

[0536] [ka]

[0537] To a stirred solution of (R)-2-(1-(tert-butoxycarbonyl)azetidin-2-yl)acetic acid (250 mg, 1.16 mmol) in THF (10 mL) was added BH3·THF (3.5 mL, 3.5 mmol, 1 M in THF) dropwise at 0 °C, and the resulting mixture was stirred at room temperature for 2 h. The reaction was quenched with CH3OH at 0 °C and then concentrated under reduced pressure. The crude product was purified by flash chromatography (PE / EtOAc = 5:1 to 1:1) to give the desired product (225 mg).

[0538] Step 2: (R)-2-(azetidin-2-yl)ethan-1-ol

[0539] [ka]

[0540] To a stirred solution of (R)-tert-butyl 2-(2-hydroxyethyl)azetidine-1-carboxylate (225 mg, 1.12 mmol) in DCM (4.0 mL) was added TFA (1.0 mL), and the resulting mixture was stirred at room temperature for 1 hour. The reaction mixture was concentrated and used in the next step without further purification.

[0541] Example 23 was prepared by the same procedure as described in Example 21 / Example 22 by replacing (S)-2-(azetidin-2-yl)ethan-1-ol with (R)-2-(azetidin-2-yl)ethan-1-ol to give the title product (8.7 mg). 1H NMR (500MHz, CD3OD) δ 6.93- 6.85 (m, 1H),6.59 - 6.39 (m,1H), 5.48 - 5.22(m, 1H), 4.56 -4.23 (m, 6H), 3.52- 3.33 (m, 3H),3.20 - 3.05 (m,1H), 2.75 - 1.80(m, 10H). MS (ESI, m / e) [M+H] + 597.5.

[0542] Example 24: 3-chloro-5-((11aS)-7-fluoro-5-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-11-methyl-2,3,11,11a-tetrahydro-1H-10-oxa-3a,4,6,9-tetraazanaphtho[1,8-ef]azulen-8-yl)-4-(trifluoromethyl)aniline

[0543] [ka]

[0544] Step 1: (S)-tert-butyl 2-formylpyrrolidine-1-carboxylate

[0545] [ka]

[0546] To a stirred solution of (COCl) (1.3 g, 10 mmol) in DCM (25 mL) was added DMSO (1.2 g, 15 mmol) dropwise at −78° C., and the resulting mixture was stirred at −78° C. for 30 minutes. To the above mixture was then added a solution of tert-butyl (S)-2-(hydroxymethyl)pyrrolidine-1-carboxylate in DCM (3.0 mL), dropwise, and the reaction mixture was stirred at −78° C. for 1 hour. To the above mixture was then added EtN (2.5 g, 25 mmol), and the mixture was stirred for an additional 30 minutes. The reaction was allowed to warm to room temperature and then extracted with DCM. The organic layer was washed with brine, dried over anhydrous NaSO, and concentrated under reduced pressure. The residue was used directly in the next step without further purification.

[0547] Step 2: tert-Butyl (2S)-2-(1-hydroxyethyl)pyrrolidine-1-carboxylate

[0548] [ka]

[0549] To a stirred solution of (S)-tert-butyl 2-formylpyrrolidine-1-carboxylate (5.0 mmol) in THF (20 mL) was added CHMgBr (2.5 mL, 7.5 mmol, 3 M) at −78° C. The resulting mixture was stirred at −78° C. for 15 min and then warmed to room temperature. The reaction mixture was quenched with saturated aqueous NHCl and extracted with EtOAc. The organic layer was washed with brine, dried over anhydrous NaSO, and concentrated under reduced pressure. The crude product was purified by flash chromatography (PE / EtOAc = 8:1 to 2:1) to give the desired product (940 mg).

[0550] Step 3: 1-((S)-pyrrolidin-2-yl)ethan-1-ol

[0551] [ka]

[0552] To a stirred solution of tert-butyl (2S)-2-(1-hydroxyethyl)pyrrolidine-1-carboxylate (4.4 mmol) in DCM (10 mL) was added 4N HCl (in dioxane) (5.0 mL) at room temperature, and the resulting mixture was stirred at room temperature for 2 hours. The reaction mixture was concentrated under reduced pressure. The crude product was used in the next step without further purification.

[0553] Step 4: 1-((S)-1-(5,7-dichloro-8-fluoro-2-(methylthio)pyrido[4,3-d]pyrimidin-4-yl)pyrrolidin-2-yl)ethan-1-ol

[0554] [ka]

[0555] To a stirred solution of 4,5,7-trichloro-8-fluoro-2-(methylthio)pyrido[4,3-d]pyrimidine (3.2 mmol) in DCM (30 mL) was added DIEA (1.2 g, 9.6 mmol) and 1-((S)-pyrrolidin-2-yl)ethan-1-ol (3.2 mmol) at 0 °C, and the resulting mixture was stirred at 0 °C for 30 min. The reaction mixture was concentrated and purified by flash chromatography (PE / EtOAc = 4:1 to 1:2) to give the desired product (1.1 g). MS (ESI, m / e) [M+1] + 377.1.

[0556] Step 5: (11aS)-8-chloro-7-fluoro-11-methyl-5-(methylthio)-2,3,11,11a-tetrahydro-1H-10-oxa-3a,4,6,9-tetraazanaphtho[1,8-ef]azulene

[0557] [ka]

[0558] To a stirred solution of 1-((S)-1-(5,7-dichloro-8-fluoro-2-(methylthio)pyrido[4,3-d]pyrimidin-4-yl)pyrrolidin-2-yl)ethan-1-ol (630 mg, 1.67 mmol) in THF (25 mL) was added NaH (200 mg, 5.01 mmol, 60%) at 0° C., and the resulting mixture was stirred at room temperature for 1 h. The reaction was quenched with HO and then extracted with EtOAc. The organic layer was washed with brine, dried over anhydrous NaSO, and concentrated under reduced pressure. The residue was used directly in the next step without further purification. MS (ESI, m / e) [M+H] + 341.1.

[0559] Step 6: (11aS)-8-chloro-7-fluoro-11-methyl-5-(methylsulfinyl)-2,3,11,11a-tetrahydro-1H-10-oxa-3a,4,6,9-tetraazanaphtho[1,8-ef]azulene

[0560] [ka]

[0561] To a stirred solution of (11aS)-8-chloro-7-fluoro-11-methyl-5-(methylthio)-2,3,11,11a-tetrahydro-1H-10-oxa-3a,4,6,9-tetraazanaphtho[1,8-ef]azulene (1.67 mmol) in DCM (10 mL) was added m-CPBA (340 mg, 1.67 mmol) at 0 °C, and the resulting mixture was stirred at 0 °C for 10 min. The reaction was quenched with aqueous NaSO and then extracted with DCM. The organic layer was washed with saturated aqueous NaHCO and brine, dried over anhydrous NaSO, and concentrated under reduced pressure. The residue was used directly in the next step without further purification.

[0562] Step 7: (11aS)-8-chloro-7-fluoro-5-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-11-methyl-2,3,11,11a-tetrahydro-1H-10-oxa-3a,4,6,9-tetraazanaphtho[1,8-ef]azulene

[0563] [ka]

[0564] To a stirred solution of (11aS)-8-chloro-7-fluoro-11-methyl-5-(methylsulfinyl)-2,3,11,11a-tetrahydro-1H-10-oxa-3a,4,6,9-tetraazanaphtho[1,8-ef]azulene (1.67 mmol) and ((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methanol (531 mg, 3.34 mmol) in THF (20 mL) was added LiHMDS (3.3 mL, 3.34 mmol, 1 M in THF) dropwise at 0 °C, and the resulting mixture was stirred at 0 °C for 20 min. The reaction was quenched with saturated aqueous NH4Cl and then extracted with EtOAc. The organic layer was washed with brine, dried over anhydrous Na2SO4, and concentrated under reduced pressure. The residue was purified by flash chromatography (DCM / MeOH=100:1 to 30:1) to give the desired product (320 mg). MS (ESI, m / e) [M+H] + 452.2.

[0565] Step 8: 3-chloro-5-((11aS)-7-fluoro-5-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-11-methyl-2,3,11,11a-tetrahydro-1H-10-oxa-3a,4,6,9-tetraazanaphtho[1,8-ef]azulen-8-yl)-4-(trifluoromethyl)aniline

[0566] To a mixture of (11aS)-8-chloro-7-fluoro-5-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-11-methyl-2,3,11,11a-tetrahydro-1H-10-oxa-3a,4,6,9-tetraazanaphtho[1,8-ef]azulene (60 mg, 0.13 mmol), 3-chloro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-4-(trifluoromethyl)aniline (84 mg, 0.26 mmol), Pd(dtbpf)Cl (17 mg, 0.03 mmol), and NaHCO (33 mg, 0.39 mmol), dioxane (4.0 mL) and HO (0.8 mL) were added. The reaction mixture was stirred for 2 hours at 90° C. The reaction mixture was concentrated and purified by flash chromatography (DCM / MeOH=100:1 to 5:1) and then preparative HPLC to give the title product (25.3 mg). 1 H NMR (500MHz, CD3OD) δ 6.90- 6.85 (m, 1H), 6.57- 6.41 (m, 1H),5.47 - 5.26 (m, 1H), 4.47 - 4.23(m, 3H), 4.06 -3.85 (m, 3H), 3.44- 3.24 (m, 3H),3.13 - 3.02 (m,1H), 2.41 - 1.80(m, 10H), 1.60 -1.48 (m, 3H). MS (ESI, m / e) [M+H] + 611.3.

[0567] Example 25: 3-chloro-5-(1-fluoro-12-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-5a,6,7,8,9,10-hexahydro-5H-4-oxa-3,10a,11,13-tetraazanaphtho[1,8-ab]heptalen-2-yl)-4-(trifluoromethyl)aniline

[0568] [ka]

[0569] Step 1: 5-(azepan-2-ylmethoxy)-7-chloro-8-fluoro-2-(methylthio)pyrido[4,3-d]pyrimidin-4-ol

[0570] [ka]

[0571] To a stirred solution of 5,7-dichloro-8-fluoro-2-(methylthio)pyrido[4,3-d]pyrimidin-4-ol (280 mg, 1.0 mmol) and azepan-2-ylmethanol (130 mg, 1.0 mmol) in THF (10 mL) was added NaH (140 mg, 3.5 mmol, 60%) at 0 °C, and the resulting mixture was stirred at room temperature for 1 h. The reaction mixture was quenched with HO, and then the pH was adjusted to 6 with 1 N aqueous HCl. The mixture was filtered, and the filter cake was used directly in the next step without further purification. MS (ESI, m / e) [M+H] + 372.9.

[0572] Step 2: 2-chloro-1-fluoro-12-(methylthio)-5a,6,7,8,9,10-hexahydro-5H-4-oxa-3,10a,11,13-tetraazanaphtho[1,8-ab]heptalene

[0573] [ka]

[0574] To a stirred solution of 5-(azepan-2-ylmethoxy)-7-chloro-8-fluoro-2-(methylthio)pyrido[4,3-d]pyrimidin-4-ol (1.0 mmol) in MeCN (50 mL) was added DIEA (323 mg, 2.5 mmol) and HATU (570 mg, 1.5 mmol) at room temperature, and the resulting mixture was stirred at room temperature for 1 h. The reaction mixture was concentrated and purified by flash chromatography (PE / EtOAc = 4:1 to 1:3) to give the desired product (243 mg). MS (ESI, m / e) [M+H] +355.2.

[0575] Step 3: 2-chloro-1-fluoro-12-(methylsulfinyl)-5a,6,7,8,9,10-hexahydro-5H-4-oxa-3,10a,11,13-tetraazanaphtho[1,8-ab]heptalene

[0576] [ka]

[0577] To a stirred solution of 2-chloro-1-fluoro-12-(methylthio)-5a,6,7,8,9,10-hexahydro-5H-4-oxa-3,10a,11,13-tetraazanaphtho[1,8-ab]heptalene (243 mg, 0.68 mmol) in DCM (10 mL) was added m-CPBA (138 mg, 0.68 mmol, 85%) at 0 °C, and the resulting mixture was stirred at 0 °C for 10 min. The reaction was quenched with aqueous NaSO and then extracted with DCM. The organic layer was washed with saturated aqueous NaHCO and brine, dried over anhydrous NaSO, and concentrated under reduced pressure. The residue was used directly in the next step without further purification.

[0578] Step 4: 2-chloro-1-fluoro-12-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-5a,6,7,8,9,10-hexahydro-5H-4-oxa-3,10a,11,13-tetraazanaphtho[1,8-ab]heptalene

[0579] [ka]

[0580] To a stirred solution of 2-chloro-1-fluoro-12-(methylsulfinyl)-5a,6,7,8,9,10-hexahydro-5H-4-oxa-3,10a,11,13-tetraazanaphtho[1,8-ab]heptalene (0.68 mmol) and ((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methanol (222 mg, 1.4 mmol) in THF (15 mL) was added LiHMDS (1.1 mL, 1.1 mmol, 1 M in THF) dropwise at 0 °C, and the resulting mixture was stirred at 0 °C for 20 min. The reaction was quenched with saturated aqueous NH4Cl and then extracted with EtOAc. The organic layer was washed with brine, dried over anhydrous Na2SO4, and concentrated under reduced pressure. The residue was purified by flash chromatography (DCM / MeOH=100:1 to 30:1) to give the product as a white solid (240 mg). MS (ESI, m / e) [M+H] + 466.3.

[0581] Step 5: 3-chloro-5-(1-fluoro-12-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-5a,6,7,8,9,10-hexahydro-5H-4-oxa-3,10a,11,13-tetraazanaphtho[1,8-ab]heptalen-2-yl)-4-(trifluoromethyl)aniline

[0582] To a mixture of 2-chloro-1-fluoro-12-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-5a,6,7,8,9,10-hexahydro-5H-4-oxa-3,10a,11,13-tetraazanaphtho[1,8-ab]heptalene (70 mg, 0.15 mmol), 3-chloro-5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-4-(trifluoromethyl)aniline (96 mg, 0.30 mmol, 89%), Pd(dtbpf)Cl (20 mg, 0.03 mmol), and NaHCO (38 mg, 0.45 mmol), dioxane (5.0 mL) and HO (1.0 mL) were added. The reaction mixture was stirred for 2 hours at 90° C. The reaction mixture was concentrated and purified by flash chromatography (DCM / MeOH=100:1 to 5:1) and then preparative HPLC to give the product (24.2 mg). 1 H NMR (500MHz, CD3OD) δ 6.90- 6.85 (m, 1H),6.57 - 6.41 (m,1H), 5.41 - 5.21(m, 1H), 5.14 -5.02 (m, 1H), 4.67- 4.55 (m, 1H),4.50 - 4.40 (m,1H), 4.32 - 4.08(m, 3H), 3.28 -3.15 (m, 3H), 3.06- 2.97 (m, 1H),2.40 - 1.56 (m,13H), 1.42 - 1.26(m, 3H). MS (ESI, m / e) [M+H] + 625.5.

[0583] Example 26: 5-ethynyl-6-fluoro-4-(1-fluoro-12-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-5a,6,7,8,9,10-hexahydro-5H-4-oxa-3,10a,11,13-tetraazanaphtho[1,8-ab]heptalen-2-yl)naphthalen-2-ol

[0584] [ka]

[0585] Step 1: 1-fluoro-2-(7-fluoro-3-(methoxymethoxy)-8-((triisopropylsilyl)ethynyl)naphthalen-1-yl)-12-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-5a,6,7,8,9,10-hexahydro-5H-4-oxa-3,10a,11,13-tetraazanaphtho[1,8-ab]heptalene

[0586] [ka]

[0587] 2-Chloro-1-fluoro-12-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-5a,6,7,8,9,10-hexahydro-5H-4-oxa-3,10a,11,13-tetraazanaphtho[1,8-ab]heptalene (70 mg, 0.15 mmol), ((2-fluoro-6-(methoxymethoxy)-8 To a mixture of -(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)naphthalen-1-yl)ethynyl)triisopropylsilane (154 mg, 0.30 mmol), Pd(dtbpf)Cl (20 mg, 0.03 mmol), and NaHCO (38 mg, 0.45 mmol), dioxane (5.0 mL) and HO (1.0 mL) were added. The reaction mixture was stirred at 90 °C for 2 h. The reaction mixture was concentrated and purified by flash chromatography (DCM / MeOH = 100:1 to 10:1) to give the title product (100 mg).

[0588] Step 2: 2-(8-ethynyl-7-fluoro-3-(methoxymethoxy)naphthalen-1-yl)-1-fluoro-12-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-5a,6,7,8,9,10-hexahydro-5H-4-oxa-3,10a,11,13-tetraazanaphtho[1,8-ab]heptalene

[0589] [ka]

[0590] To a solution of 1-fluoro-2-(7-fluoro-3-(methoxymethoxy)-8-((triisopropylsilyl)ethynyl)naphthalen-1-yl)-12-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-5a,6,7,8,9,10-hexahydro-5H-4-oxa-3,10a,11,13-tetraazanaphtho[1,8-ab]heptalene (100 mg, 0.12 mmol) in DMF (4 mL) was added CsF (93 mg, 0.60 mmol), and the resulting mixture was stirred at room temperature for 1 hour. The reaction mixture was diluted with EtOAc and HO and then extracted with EtOAc. The organic layer was washed with brine, dried over anhydrous NaSO, and concentrated under reduced pressure. The crude product was used directly in the next step without further purification. MS (ESI, m / e) [M+H] + 660.5.

[0591] Step 3: 5-ethynyl-6-fluoro-4-(1-fluoro-12-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-5a,6,7,8,9,10-hexahydro-5H-4-oxa-3,10a,11,13-tetraazanaphtho[1,8-ab]heptalen-2-yl)naphthalen-2-ol

[0592] To a solution of 2-(8-ethynyl-7-fluoro-3-(methoxymethoxy)naphthalen-1-yl)-1-fluoro-12-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-5a,6,7,8,9,10-hexahydro-5H-4-oxa-3,10a,11,13-tetraazanaphtho[1,8-ab]heptalene (0.12 mmol) in DCM (4.0 mL) was added 4N HCl (in dioxane) (1.0 mL) at 0° C., and the resulting mixture was stirred at room temperature for 1 hour. The reaction mixture was concentrated and basified with DIEA. The residue was purified by flash chromatography (DCM / MeOH=100:1 to 10:1) and then preparative HPLC to give the product (32.8 mg). 1 H NMR (500MHz, CD3OD) δ 7.86- 7.80 (m, 1H),7.36 - 7.12 (m,3H), 5.41 - 5.22(m, 1H), 5.18 -5.05 (m, 1H), 4.68- 4.60 (m, 1H),4.54 - 4.44 (m,1H), 4.28 - 4.20(m, 2H), 4.20 -4.06 (m, 1H), 3.53- 3.32 (m, 2H),3.29 - 3.19 (m,3H), 3.10 - 2.98(m, 1H), 2.45 -1.32 (m, 14H). MS (ESI, m / e) [M+H] + 616.5.

[0593] Example 27: 2-amino-4-((S)-10-chloro-8-fluoro-6-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-2,3,12,12a-tetrahydro-1H-pyrrolo[2',1':3,4][1,4]oxazepino[5,6,7-de]quinazolin-9-yl)-7-fluorobenzo[b]thiophene-3-carbonitrile

[0594] [ka]

[0595] Step 1: (S)-Pyrrolidin-2-ylmethanol

[0596] [ka]

[0597] A mixture of (S)-tert-butyl 2-(hydroxymethyl)pyrrolidine-1-carboxylate (1 g, 4.98 mmol) in HCl (4 M) / dioxane (10 mL) was stirred at room temperature for 3 hours. The mixture was then concentrated under reduced pressure to give the crude product (1.1 g, crude), which was used directly without further purification. MS (ESI, m / e) [M+H] + 102.2.

[0598] Step 2: (S)-(1-(7-bromo-6-chloro-5,8-difluoro-2-(methylthio)quinazolin-4-yl)pyrrolidin-2-yl)methanol

[0599] [ka]

[0600] To a solution of 7-bromo-4,6-dichloro-5,8-difluoro-2-(methylthio)quinazoline (800 mg, 2.23 mmol) and (S)-pyrrolidin-2-ylmethanol (338 mg, 3.35 mmol) in DCM (15 mL) was added DIEA (863 mg, 6.69 mmol). The mixture was stirred at room temperature overnight. The mixture was then concentrated under reduced pressure, and the residue was purified by silica gel column chromatography (12 g, eluted with PE / EtOAc = 100%:0% to 50%:50%) to give the title compound (735 mg). MS (ESI, m / e) [M+H] + 424.1.

[0601] Step 3: (S)-9-Bromo-10-chloro-8-fluoro-6-(methylthio)-2,3,12,12a-tetrahydro-1H-pyrrolo[2',1':3,4][1,4]oxazepino[5,6,7-de]quinazoline

[0602] [ka]

[0603] To a solution of (S)-(1-(7-bromo-6-chloro-5,8-difluoro-2-(methylthio)quinazolin-4-yl)pyrrolidin-2-yl)methanol (735 mg, 1.737 mmol) in THF (10 mL) was added LiHMDS (4.34 mL, 4.34 mmol, 1 M in hexane) at 0° C. The mixture was then stirred at room temperature overnight. The mixture was concentrated under reduced pressure, and the residue was purified by silica gel column chromatography (12 g, eluted with PE / EtOAc = 100%:0% to 0%:100%) to give the title compound (486 mg). MS (ESI, m / e) [M+H] + 404.1.

[0604] Step 4: (S)-9-Bromo-10-chloro-8-fluoro-6-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-2,3,12,12a-tetrahydro-1H-pyrrolo[2',1':3,4][1,4]oxazepino[5,6,7-de]quinazoline

[0605] [ka]

[0606] (S)-9-Bromo-10-chloro-8-fluoro-6-(methylthio)-2,3,12,12a-tetrahydro-1H-pyrrolo[2',1':3,4][1,4]oxazepino[5,6,7-de]quinazoline (486 mg, 1.21 mmol) was placed in DCM (10 mL). m-CPBA (237 mg, 1.38 mmol) was added portionwise to the solution at 0 °C. The mixture was stirred at 0 °C for 2 hours. ((2R,7aS)-2-Fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methanol (575 mg, 3.60 mmol) was placed in THF (10 mL). LiHMDS (2.4 mL, 2.40 mmol) was added portionwise to the solution at 0 °C. The mixture was stirred at room temperature for 30 minutes, and the mixture was added to the above DCM solution. The mixture was stirred overnight at room temperature, quenched with MeOH (5 mL), and concentrated under reduced pressure. The residue was purified by silica chromatography column (eluted with DCM / MeOH = 90%:10%) to obtain the title compound (450 mg). MS (ESI, m / e) [M+H] + 515.1.

[0607] Step 5: 2-amino-4-((S)-10-chloro-8-fluoro-6-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-2,3,12,12a-tetrahydro-1H-pyrrolo[2',1':3,4][1,4]oxazepino[5,6,7-de]quinazolin-9-yl)-7-fluorobenzo[b]thiophene-3-carbonitrile

[0608] K3PO4 (237 mg, 1.12 mmol), dioxane / water (10 mL / 2 mL), (S)-9-bromo-10-chloro-8-fluoro-6-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-2,3,12,12a-tetrahydro-1H-pyrrolo[2',1':3,4][1,4]oxazepino[5,6,7-de]quinazoline (230 ... A mixture of (3-cyano-7-fluoro-4-(4,4,5,5-tetramethyl-1,3-dioxolan-2-yl)benzo[b]thiophen-2-yl)carbamate (374 mg, 0.895 mmol) and 1,1′-bis(di-t-butylphosphino)ferrocenepalladium dichloride (146 mg, 0.223 mmol) was stirred at 80° C. for 2 hours. The reaction was cooled to room temperature, concentrated under reduced pressure, and purified by silica chromatography column (12 g, eluted with DCM / MeOH=90%:10%) to give the crude product, which was dissolved in HCl (4 M) / dioxane (10 mL) and stirred at room temperature for 3 hours. The mixture was then concentrated under reduced pressure and further purified by preparative HPLC to give the title compound (16.2 mg). 1 H NMR (500MHz, DMSO-d6) δ 8.07(s, 2H), 7.26-7.11 (m, 2H),5.37-5.17 (m, 1H), 4.80-4.65(m, 1H), 4.23-4.17 (m, 1H),4.13-3.98 (m, 3H), 3.91-3.75(m, 2H), 3.13-2.99 (m, 3H), 2.86-2.79 (m, 1H), 2.27-1.66(m, 10H). MS (ESI, m / e) [M+H] + 627.3.

[0609] Example 28: 2-amino-4-((2R,12aS)-10-chloro-8-fluoro-6-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-2-methyl-2,3,12,12a-tetrahydro-1H-pyrrolo[2',1':3,4][1,4]oxazepino[5,6,7-de]quinazolin-9-yl)-7-fluorobenzo[b]thiophene-3-carbonitrile

[0610] [ka]

[0611] Example 28 was prepared by a similar procedure as described in Example 27 by replacing (S)-pyrrolidin-2-ylmethanol with ((2S,4R)-4-methylpyrrolidin-2-yl)methanol to afford the title product (14.5 mg). 1 H NMR (500MHz, DMSO-d6) δ 8.07(s, 2H), 7.28-7.12 (m, 2H),5.33-5.12 (m, 1H), 4.79-4.65(m, 1H), 4.33-4.18 (m, 2H),4.14-3.95 (m, 3H), 3.45-3.40(m, 1H), 3.12-2.97 (m, 3H),2.85-2.79 (m, 1H), 2.15-1.69(m, 8H), 1.13-1.02 (m, 3H). MS(ESI, m / e) [M+H] + 641.3.

[0612] Example 29: 2-amino-4-((2R,12aS)-10-chloro-8-fluoro-6-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-2-hydroxy-2,3,12,12a-tetrahydro-1H-pyrrolo[2',1':3,4][1,4]oxazepino[5,6,7-de]quinazolin-9-yl)-7-fluorobenzo[b]thiophene-3-carbonitrile

[0613] [ka]

[0614] Example 29 was prepared by a similar procedure as described in Example 27 by replacing (S)-pyrrolidin-2-ylmethanol with (3R,5S)-5-(hydroxymethyl)pyrrolidin-3-ol to afford the title product (5.8 mg). 1 H NMR (500MHz, DMSO-d6) δ 8.07(s, 2H), 7.28-7.07 (m, 2H),5.35-5.12 (m, 1H), 4.80-4.72(m, 1H), 4.47-3.95 (m, 6H),3.78-3.63 (m, 1H), 3.13-2.98(m, 3H), 2.90-2.75 (m, 1H),2.45-1.47 (m, 8H). MS (ESI, m / e) [M+H] + 643.3.

[0615] Example 30: (8aS)-5-(2-amino-3-cyano-7-fluorobenzo[b]thiophen-4-yl)-6-chloro-4-fluoro-2-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-8,8a,9,10,11,12-hexahydropyrido[2',1':3,4][1,4]oxazepino[5,6,7-de]quinazoline-10-carbonitrile

[0616] [ka]

[0617] Example 30 was prepared by a similar procedure as described in Example 27 by replacing (S)-pyrrolidin-2-ylmethanol with tert-butyl (2S)-4-cyano-2-(hydroxymethyl)piperidine-1-carboxylate to afford the title product (5 mg). 1H NMR (500MHz, DMSO-d6) 8.09 (s,2H), 7.25 -7.11 (m, 2H),5.35 -5.15 (m, 1H),5.10-4.95 (m, 1H), 4.64-4.39(m, 2H), 4.20-3.93 (m, 3H),3.58-3.48 (m, 1H), 3.23-3.15(m, 1H), 3.11-2.98 (m, 2H),2.90-2.79 (m, 1H), 2.19-1.97(m, 5H), 1.91-1.74 (m, 4H),1.29-1.07 (m, 2H). MS (ESI, m / e) [M+H] + 666.4.

[0618] Example 31: 2-amino-4-((8aS,10R)-6-chloro-4-fluoro-2-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-10-hydroxy-8,8a,9,10,11,12-hexahydropyrido[2',1':3,4][1,4]oxazepino[5,6,7-de]quinazolin-5-yl)-7-fluorobenzo[b]thiophene-3-carbonitrile [ka]

[0619] Step 1: tert-Butyl (2S,4R)-4-hydroxy-2-(hydroxymethyl)piperidine-1-carboxylate [ka]

[0620] To a solution of 1-(tert-butyl)-2-methyl (2S,4R)-4-hydroxypiperidine-1,2-dicarboxylate (2.6 g, 10 mmol) in THF (40 mL) was added LiBH (2 M in THF, 7 mL, 14 mmol) at 0 °C. The mixture was stirred at room temperature overnight. The mixture was quenched with NH Cl (aq) and extracted with EtOAc. The organic phase was then concentrated under reduced pressure to give the crude product. The residue was purified by silica gel column chromatography (20 g, eluting with DCM / MeOH = 100%:0% to 90%:10%) to give the title compound (2.0 g).

[0621] Step 2: (2S,4R)-2-(hydroxymethyl)piperidin-4-ol [ka]

[0622] To a suspension of tert-butyl (2S,4R)-4-hydroxy-2-(hydroxymethyl)piperidine-1-carboxylate (1.5 g, 6.5 mmol) in DCM (10 mL) was added TFA (10 mL) dropwise. The mixture was stirred at room temperature for 1 hour. The mixture was then concentrated under reduced pressure to give the crude product (1.6 g, crude), which was used directly without further purification.

[0623] Example 31 was prepared by a similar procedure as described in Example 27 by replacing (S)-pyrrolidin-2-ylmethanol with (2S,4R)-2-(hydroxymethyl)piperidin-4-ol to afford the title product (5 mg). 1H NMR (500MHz, DMSO-d6) δ 8.08(s, 2H), 7.33-7.09 (m, 2H),5.39-5.18 (m, 1H), 4.98-4.89(m, 1H), 4.48-4.39 (m, 2H),4.16-3.77 (m, 4H), 3.06- 2.96 (m, 3H),2.84 (m, 1H), 2.15-1.95(m, 6H), 1.88-1.74 (m, 3H),1.45-1.35 (m, 1H). MS (ESI, m / e) [M+H] + 657.3.

[0624] Example 32: 5-ethynyl-6-fluoro-4-((5aS,6aS,7aS)-1-fluoro-9-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-5,5a,6,6a,7,7a-hexahydro-4-oxa-3,7b,8,10-tetraazacyclopropa[α]naphtho[1,8-gh]azulen-2-yl)naphthalen-2-ol [ka]

[0625] Example 32 was prepared by a similar procedure as described in Example 21 by replacing (S)-2-(azetidin-2-yl)ethan-1-ol with ((1S,3S,5S)-2-azabicyclo[3.1.0]hexan-3-yl)methanol to afford the title product (21 mg). 1H NMR (500MHz, CD3OD) δ 7.89- 7.78 (m, 1H),7.38 - 7.17 (m,3H), 5.49 - 5.30(m, 1H), 4.81 -4.73 (m, 1H), 4.59- 4.49 (m, 2H),4.46 - 4.34 (m,1H), 4.26 - 4.14(m, 1H), 3.92 -3.82 (m, 1H), 3.69- 3.42 (m, 4H),3.26 - 3.15 (m,1H), 2.87 - 2.73(m, 1H), 2.56 -2.34 (m, 2H), 2.33- 2.21 (m, 1H),2.20 - 2.08 (m,2H), 2.08 - 1.92(m, 1H), 1.92 -1.82 (m, 1H), 1.81- 1.71 (m, 1H),1.39 - 1.26 (m,1H), 0.74 - 0.63(m, 1H). MS (ESI, m / e) [M+1] + 600.8.

[0626] Example 33: 3-chloro-5-((5S,5aS)-1-fluoro-11-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-5-methyl-5,5a,6,7,8,9-hexahydro-4-oxa-3,9a,10,12-tetraazabenzo[4,5]cyclohepta[1,2,3-de]naphthalen-2-yl)-4-(trifluoromethyl)aniline [ka]

[0627] Example 33 was prepared by a similar procedure as described in Example 23 by replacing (R)-2-(azetidin-2-yl)ethan-1-ol with (S)-1-((S)-piperidin-2-yl)ethan-1-ol to afford the title product (4.8 mg). 1H NMR (500MHz, DMSO-d6) δ 6.85(s, 1 H), 6.48-6.30(m, 3H), 5.36-5.20 (m, 1H), 5.01-4.96(m, 1 H),4.54-4.49(m, 1 H), 4.15-4.09(m,1 H), 4.03-3.97(m, 1 H),3.91-3.84(m, 1 H), 3.18-3.01(m,4 H), 2.84-2.76(m, 1 H),2.20-1.95(m, 3 H), 1.89-1.53(m,8 H), 1.48-1.29(m, 4H). MS(ESI, m / e) [M+H] + 625.5.

[0628] Example 34: 5-ethynyl-6-fluoro-4-((5S,5aS)-1-fluoro-11-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-5-methyl-5,5a,6,7,8,9-hexahydro-4-oxa-3,9a,10,12-tetraazabenzo[4,5]cyclohepta[1,2,3-de]naphthalen-2-yl)naphthalen-2-ol [ka]

[0629] Example 34 was prepared by a similar procedure as described in Example 21 by replacing (S)-2-(azetidin-2-yl)ethan-1-ol with (S)-1-((S)-piperidin-2-yl)ethan-1-ol to afford the title product (8.8 mg). 1 H NMR (500MHz, DMSO-d6) δ 10.31-10.01(m,1 H), 8.01-7.94(m, 1 H),7.52-7.46(m, 1 H), 7.23-7.20(m,1 H), 7.16-7.07(m, 1 H),5.37-4.43(m, 3H), 4.15-3.76(m,4H), 3.21-2.98(m, 4H),2.87-2.80(m, 1H), 2.21-1.37(m,15H). MS (ESI, m / e) [M+H]+ 616.6.

[0630] Example 35: (5aS,8S)-2-(8-ethynyl-7-fluoro-3-hydroxynaphthalen-1-yl)-1-fluoro-11-(((2R,7aS)-2-fluorotetrahydro-1H-pyrrolidin-7a(5H)-yl)methoxy)-5,5a,6,7,8,9-hexahydro-4-oxa-3,9a,10,12-tetraazabenzo[4,5]cyclohepta[1,2,3-de]naphthalen-8-ol [ka]

[0631] Example 35 was prepared by a similar procedure as described in Example 15 / 16 by replacing (S)-piperidin-2-ylmethanol with (3S,6S)-6-(hydroxymethyl)piperidin-3-ol to give the title product (3.5 mg). 1 H NMR (500MHz, CD3OD) δ 7.86- 7.81 (m, 1H),7.33 - 7.15 (m,3H), 5.50 - 5.34(m, 2H), 4.62 -4.55 (m, 2H), 4.50- 4.10 (m, 1H),4.39 - 4.27 (m,1H), 4.16 - 4.08(m, 1H), 4.00 -3.92 (m, 1H), 3.61- 3.35 (m, 4H),3.21 - 3.11 (m,1H), 2.53 - 2.18(m, 4H), 2.17 -1.90 (m, 5H) ,1.81 - 1.70 (m,1H). MS (ESI, m / e) [M+H] + 618.5.

[0632] Assay KRAS WT and KRAS G12V probe displacement assay

[0633] This assay was used to identify compounds that could bind to GDP-bound KRAS protein and displace a biotinylated probe that occupies the KRAS binding site. GST-tagged GDP-bound WT KRAS (amino acids 1–169) and GST-tagged GDP-bound KRAS G12V (amino acids 1–169) were expressed in-house in E. coli and purified. All proteins and reactions were prepared in assay buffer containing 50 mM HEPES (pH 7.5), 50 mM NaCl, 1 mM MgCl2, 1 mM TCEP, 0.01% BSA, and 0.008% Brij-35. Purified WT KRAS (3 nM final concentration) or KRAS G12V protein (2 nM final concentration) was incubated with 3-fold serially diluted compounds in an assay plate (384-well black microplate, Corning). The plate was incubated at 24°C for 1 h. After incubation, biotinylated probe 1 for WT KRAS (60 nM final assay concentration) and biotinylated probe 2 for KRAS G12V (2.5 nM final assay concentration) were added to the assay plate, respectively. After 1 h of incubation at 24 °C, anti-GST mAb-Tb Cryptate (Cisbio) and streptavidin-XL665 (Cisbio) were added and incubated for another 1 h at 24 °C. TR-FRET signals (excitation 337 nm, emission 665 nm / 620 nm) were read on a BMG PHERAstar FSX instrument. The percentage inhibition of KRAS protein binding to the biotinylated probe in the presence of increasing concentrations of compound was calculated based on the ratio of fluorescence at 665 nm to fluorescence at 620 nm. The IC of each compound was calculated by fitting the data to a four-parameter logistic model using Dotmatics. 50 The value was calculated.

[0634] KRAS WT and KRAS G12D probe displacement assay

[0635] This assay was used to identify compounds that could bind to GDP-bound KRAS protein and displace a biotinylated probe that occupies the KRAS binding site. GST-tagged GDP-bound WT KRAS (amino acids 1–188) and GST-tagged GDP-bound KRAS G12D (amino acids 1–188) were expressed in-house in E. coli and purified. All proteins and reactions were prepared in assay buffer containing 50 mM HEPES (pH 7.5), 50 mM NaCl, 1 mM MgCl2, 1 mM TCEP, 0.01% BSA, and 0.008% Brij-35. Purified WT KRAS (3 nM final concentration) or KRAS G12D protein (0.5 nM final concentration) was incubated with 3-fold serially diluted compounds in an assay plate (384-well black microplate, Corning). The plate was incubated at 24°C for 1 h. After incubation, biotinylated probe 1 for WT KRAS (60 nM final assay concentration) and biotinylated probe 2 for KRAS G12D (4 nM final assay concentration) were added to the assay plate, respectively. After 1 h of incubation at 24 °C, anti-GST mAb-Tb Cryptate (Cisbio) and streptavidin-XL665 (Cisbio) were added and incubated for another 1 h at 24 °C. TR-FRET signals (excitation 337 nm, emission 665 nm / 620 nm) were read on a BMG PHERAstar FSX instrument. The percentage inhibition of KRAS protein binding to the biotinylated probe in the presence of increasing concentrations of compound was calculated based on the ratio of fluorescence at 665 nm to fluorescence at 620 nm. The IC of each compound was calculated by fitting the data to a four-parameter logistic model using Dotmatics. 50 The value was calculated.

[0636] KRAS G12V pERK assay

[0637] The SW620 cell line was used in this experiment. Cells were maintained in RPMI 1640 supplemented with 10% fetal bovine serum (Thermo Fisher) and 50 units / mL penicillin and streptomycin (Thermo Fisher) and stored at 37°C in a humidified atmosphere of 5% CO2 in air. Cells were recovered from cryopreserved stocks generated within 30 passages of the original cells purchased. 40,000 cells per well were seeded into a 96-well plate and incubated overnight. Cells were treated with a 10-point dilution series. Final compound concentrations ranged from 0 to 10 μM. After 2 hours of compound treatment, cells were lysed, and pERK1 / 2 (THR202 / TYR204) levels in the cell lysates were detected using an HTRF kit (Cisbio). Briefly, a total of 16 μL of cell lysate from each well of the 96-well plate was transferred to a 384-well white assay plate. Lysates from each well were incubated overnight at room temperature in the dark with 2 μL of Eu3+-cryptate (donor)-labeled anti-phospho-ERK1 / 2 and 2 μL of D2 (acceptor)-labeled anti-phospho-ERK1 / 2 antibody (Cisbio). When the donor and acceptor are in close proximity, excitation of the donor by a laser induces fluorescence resonance energy transfer (FRET) to the acceptor, causing the acceptor to fluoresce at a wavelength of 655 nm. FRET signals were measured using a PHERAstar FSX reader (BMG Labtech). IC50 determinations were performed by fitting the curve of percent inhibition versus the logarithm of inhibitor concentration using Dotmatics.

[0638] KRAS G12D pERK assay

[0639] The AsPC-1 cell line was used in this experiment. Cells were maintained in RPMI-1640 supplemented with 10% fetal bovine serum (Thermo Fisher) and 50 units / mL penicillin and streptomycin (Thermo Fisher) and stored at 37°C in a humidified atmosphere of 5% CO2 in air. Cells were recovered from cryopreserved stocks generated within 30 passages of the original cells purchased. Thirty thousand cells per well were seeded into a 96-well plate and incubated overnight. Cells were treated with a 10-point dilution series. Final compound concentrations ranged from 0 to 10 μM. After 2 hours of compound treatment, cells were lysed, and pERK1 / 2 (THR202 / TYR204) levels in the cell lysates were detected using an HTRF kit (Cisbio). Briefly, a total of 16 μL of cell lysate from each well of the 96-well plate was transferred to a 384-well white assay plate. Lysates from each well were incubated overnight at room temperature in the dark with 2 μL of Eu3+-cryptate (donor)-labeled anti-phosphorylated ERK1 / 2 and 2 μL of D2 (acceptor)-labeled anti-phosphorylated ERK1 / 2 antibody (Cisbio). When the donor and acceptor are in close proximity, excitation of the donor by a laser induces fluorescence resonance energy transfer (FRET) to the acceptor, causing the acceptor to fluoresce at a wavelength of 655 nm. FRET signals were measured using a PHERAstar FSX reader (BMG Labtech). IC was calculated by fitting a curve of percent inhibition versus the logarithm of inhibitor concentration using Dotmatics. 50 The decision was carried out.

[0640] Preparation and crystallization of KRAS G12D protein

[0641] Purification of KRAS G12D protein

[0642] KRAS G12D (1-169 aa) was cloned into the pET28a vector. The gene was placed in frame with an N-terminal 6xHis tag and a Sumo tag. The construct was transformed into BL21(DE3) cells. Protein expression was measured when cells reached an OD of 0.6. 600Upon reaching a pH of 10, 1-thio-β-D-galactopyranoside (IPTG) was added to a final concentration of 200 μM, followed by overnight incubation at 16°C for induction. Bacteria were harvested by centrifugation (4000 rpm, 20 min, 4°C), and 1 liter of cell paste was resuspended in 30 ml of 50 mM Tris (pH 8.0), 300 mM NaCl, 20 mM imidazole, and 5 mM MgCl2 supplemented with two EDTA-free protease inhibitor cocktail tablets (Roche Diagnostics). Protein was purified on a His-trap HP column (Cytiva) according to standard protocols. The N-terminal His-Sumo tag was cleaved by overnight digestion with ULP1 protease, and the ULP1 His-Sumo tag was removed by reloading onto a His-trap HP column (Cytiva). The protein was further purified by gel filtration using a HiLoad 16 / 600 Superdex 75 pg (Cytiva) column equilibrated with 20 mM Tris (pH 8.0), 100 mM NaCl, and 5 mM MgCl. The protein solution was concentrated to 30–40 mg / ml for crystallization experiments.

[0643] Crystallization of KRAS G12D

[0644] Co-crystals of KRAS G12D with small molecule inhibitors were grown at 20°C using the sitting drop vapor diffusion method by mixing 1 μL of protein (40 mg / ml) with an equal volume of crystallization buffer. Crystals appeared in drops containing 1.0 M LiCl, 0.1 M citric acid (pH 5.0), and 20% PEG6000. Diffraction data were collected at beamline BL10U2 of the Shanghai Synchrotron Radiation Facility.

[0645] Metabolic stability in liver microsomes from different species

[0646] Liver microsomes were first mixed with NADPH to obtain final concentrations of 0.5 mg / mL and 1 mM microsomes and NADPH, respectively. Test compounds were added to the cultures at a final concentration of 1 μM and incubated at 37°C. The incubation was initiated by the addition of NADPH to the cultures. 20 μL aliquots were taken from the cultures at 0, 15, 30, 45, and 60 minutes after the start of incubation. The reaction was stopped by the addition of cold acetonitrile containing analytical IS. The samples were centrifuged at 4000 rpm for 5 minutes and then analyzed by LC-MS / MS.

[0647] The peak areas of samples at various time points were determined from the extracted ion chromatograms and then plotted to calculate metabolic stability. The slope value (k) was determined by linear regression of the curve of the natural logarithm of the remaining percentage of parent drug versus incubation time. The in vitro half-life (in vitro t 1 / 2 ) was determined from the slope value.

number

number

[0648] A control compound (verapamil) was included in the assay to ensure data consistency. A negative control (identical experimental setup but without NADPH in the culture system) was used to exclude misleading factors caused by the instability of the chemical itself.

[0649] CYP (cytochrome P450) enzyme inhibition assay in human liver microsomes.

[0650] Incubations were performed in a 96-well plate. 1 μL of the test compound working solution or vehicle was added to 179 μL of human liver microsomes fortified with substrates for CYP1A2 (40 μM phenacetin), 2C9 (6 μM diclofenac), 2C19 (50 μM (S)-mephenytoin), 2D6 (10 μM dextromethorphan), and 3A4 (1 μM midazolam or 50 μM testosterone). The incubation plate was preheated in a water bath at 37°C for 5 minutes, after which the reaction was initiated by the addition of 20 μL of 10 mM NADPH solution. The reaction was performed in a 37°C water bath.

[0651] The reaction was stopped at predetermined time points by adding 300 μL of quenching solution (acetonitrile containing an internal standard) to each well. The sample plate was vortexed for 1 minute and centrifuged at 3000 g for 10 minutes. 100 μL of the supernatant was transferred to a new 96-well plate and then mixed with 100 μL of water for analysis by LC-MS / MS and subsequent data processing (i.e., determination of percent inhibition or IC50 at 10 μM).

[0652] Time-dependent cytochrome P450 (CYP) enzyme inhibition assay (TDI) in human liver microsomes

[0653] The TDI assay involves pre-incubation of 0.1 mg mL-1 human liver microsomes with 10 uM test compound and a positive control in the presence or absence of 1 mM NADPH for 30 min at 37°C ("inactivation incubation"). After the preincubation period, residual CYP activity was measured by adding substrate (CYP1A2: 40 μM phenacetin; CYP2B6: 50 μM bupropion; CYP2C8: 5 μM paclitaxel; CYP2C9: 6 μM diclofenac; CYP2C19: 50 μM (S)-mephenytoin; CYP2D6: 10 μM dextromethorphan; CYP3A: 1 μM midazolam or 50 μM testosterone) and NADPH to the preincubation mixture, followed by an "activity incubation" of an additional 20 min for CYP1A2, 2B6, 2C19, and 2D6, an additional 10 min for CYP2C8 and CYP3A (testosterone), an additional 6 min for CYP2C9, and an additional 5 min for CYP3A (midazolam). All reactions are stopped by the addition of ice-cold acetonitrile containing an internal standard and then centrifuged for LC-MS / MS analysis.

[0654] Bidirectional permeability assay in MDCKII-MDR1 cell monolayers

[0655] First, MDCKII-MDR1 cells were prepared in cell seeding medium. 50 μL of the cultured cell suspension was added to each well of a pre-prepared Transwell plate. The plate was incubated for 4–8 days. The medium was changed every other day. Prior to permeability measurements, the integrity of the cell monolayer was assessed by electrical resistance.

[0656] To determine the rate of drug transport from apical to basolateral, 125 μL of the working solution of the test compound was added to the Transwell insert (apical compartment), and 50 μL of the sample (D0 sample) was immediately transferred from the apical compartment to a new 96-well plate. To determine the rate of drug transport from basolateral to apical, 285 μL of the working solution of the compound was added to the receiver plate well (basolateral compartment), and 50 μL of the sample (D0 sample) was immediately transferred from the basolateral compartment to a new 96-well plate. The plate was incubated at 37°C for 2 hours. At the end of the transport period, 50 μL was directly transferred from the apical and basolateral wells and transferred to a new plate. 200 μL of cold acetonitrile containing internal standards (IS: 2 μM ketoprofen, 200 nM labetalol, 200 nM caffeine, and 100 nM alprazolam) was then added. Vortex for 5 minutes. Centrifuge the sample at 3,220 g for 20 minutes. Dilute an aliquot of 100 μL of the supernatant with 100 μL of ultrapure HO and use the mixture for LC / MS / MS analysis. All incubations are performed in duplicate. For the MDCKII-MDR1 drug transport assay, the apparent permeability (Papp) in centimeters per second can be calculated using the following formula:

number

number

number

[0657] In the formula, V A is the volume in the acceptor well (mL) (0.235 mL for Ap → Bl efflux and 0.075 mL for Bl → Ap efflux), and V D is the volume (mL) in the donor well (0.075 mL for Ap→Bl outflow and 0.235 mL for Bl→Ap outflow).

[0658] Intrinsic clearance in hepatocytes of different species

[0659] 10 mM stock solutions of test compounds and positive controls are prepared in the appropriate solvent (DMSO). Incubation medium (Williams E medium supplemented with GlutaMAX) is placed in a 37°C water bath and allowed to warm for at least 15 minutes before use. 10 mM test compounds and positive controls are diluted to 100 μM by mixing 198 μL of 50% acetonitrile / 50% water and 2 μL of the 10 mM stock solution in separate conical tubes. 198 μL of cryopreserved hepatocytes (0.5 x 10 6A total of 100 μM of test compound or positive control (viable cells / mL) is pipetted into each well of an uncoated 96-well plate. 2 μL of 100 μM test compound or positive control is pipetted into each well of the uncoated 96-well plate to initiate the reaction. The final concentration of test compound or control compound is 1 μM. The plate is returned to the incubator and placed on an orbital shaker. The contents of the wells are removed in 25 μL aliquots at 0, 15, 30, 60, 90, and 120 minutes. The aliquots are then mixed with 6 volumes (150 μL) of cold acetonitrile containing IS (2 μM ketoprofen, 200 nM labetalol, 200 nM caffeine, and 100 nM alprazolam) to stop the reaction. Centrifuge at 3,220 g for 30 minutes. A 100 μL aliquot of the supernatant is used for LC / MS / MS analysis. The supernatant may be diluted with ultrapure water depending on the LC-MS signal response and peak shape. All incubations are performed in duplicate.

[0660] All calculations were performed using Microsoft Excel. Peak areas were determined from extracted ion chromatograms. The in vitro half-life (t 1 / 2 ) is determined by regression analysis of the curve of percent parent compound disappearance versus time.

[0661] In vitro half-life (in vitro t 1 / 2 ) is determined from the slope value. In vitro 1 / 2 =0.693 / k In vitro 1 / 2 In vitro intrinsic clearance (in vitro CL) (min) int ,μL / min / 10 6 The conversion to individual cells is performed using the following formula: In vitro CL int =kV / N

[0662] V = incubation volume (0.2 mL); N = number of hepatocytes per well (0.1 x 10 6 individual cells).

[0663] PK studies in mice and rats

[0664] The pharmacokinetics of compounds were evaluated in male CD-1 mice or SD-JVC rats by intravenous and oral administration. For intravenous administration, test compounds were dissolved in DMA:30% Solutol HS 15 (w / v):saline (20:20:60, by volume) and injected via the tail vein at a dose of 1 mg / kg. For oral administration, test compounds were dissolved in 0.5% MC or PEG400 / Phosal 50 PG / EtOH (30 / 60 / 10, by volume) and administered to mice by gavage at 10 mg / kg or 30 mg / kg. Animals were grouped and treated according to body weight. At post-dose time points (5 (IV only), 15, and 30 minutes, and 1, 2, 4, 8, and 24 hours after administration), rat blood samples were collected from the JVC. Mice were anesthetized with isoflurane, and blood samples were collected via retro-orbital bleed. Blood samples were collected in 1.5 mL EP tubes coated with EDTA.K2. Approximately 50 μL of blood (mice) and 150 μL of blood (rats) were collected at each time point, placed on ice, and then centrifuged at 5600 rpm at 4°C for 7 minutes to obtain plasma. The plasma was transferred to a new tube and temporarily stored at -20°C or on dry ice. Samples were stored at -80°C until ex vivo PK assay.

[0665] Plasma concentrations were determined using the following sample processing method and measurement conditions: 10 μL of sample aliquot was added to ACN together with 200 μL of IS (terfenadine, 5 ng / mL). The mixture was vortexed for 1 minute and centrifuged at 4000 rpm for 10 minutes at 4°C. 80 μL of supernatant aliquot was diluted with 80 μL of water, and the mixed sample was injected into a liquid chromatography-tandem mass spectrometer (LC-MS / MS, Triple Quad 5500) for analysis. Sample volume injected: 2 μL. Monitor: MRM; Column: Advanced Materials Technology, HALO AQ-C18 2.7 μm 90 Å, 50*2.1 mm; Column temperature: 40 °C; Mobile phase A: HO-0.1% FA, Mobile phase B: ACN-0.1% FA; Gradient program: 15% B → 15% B (0 min to 0.3 min), 15% B → 90% B (0.3 min to 1.0 min), 90% B → 90% B (1.0 min to 1.8 min), 90% B → 30% B (1.8 min to 2.0 min), 30% B → 30% B (2.0 min to 2.5 min).

[0666] PD research in SW1990:

[0667] 5 x 10 cells per 200 µL of PBS / Matrigel were placed on the right flank of female NCG mice. 6 SW1990 cells were subcutaneously implanted. After inoculation, the tumor size was approximately 350-450 mm. 3 Once the average volume reached 100 mg / kg, mice were randomized into treatment groups. Randomized mice were orally administered a single dose of vehicle consisting of 0.5% MC or various doses (e.g., 30, 50, or 100 mg / kg) of test compound. Plasma was collected at 0.5, 2, 4, and 7 hours, and tumors were harvested 7 hours after administration to determine exposure levels. Tumor fragments were flash-frozen in homogenization tubes using liquid nitrogen and homogenized with T-PER Tissue Protein Extraction Buffer freshly supplemented with protease and phosphatase inhibitors before use. Tumor lysates were then analyzed for ERK1 / 2 phosphorylation.

[0668] Efficacy studies in SW1990:

[0669] 5 x 10 cells per 200 µL of PBS / Matrigel were placed on the right flank of female NCG mice. 6 SW1990 cells were subcutaneously transplanted. After inoculation, the tumor size was approximately 150-250 mm. 3 Once the average tumor volume reached 100 mg / kg, the mice were randomized into treatment groups. Randomized mice were orally administered a vehicle consisting of 0.5% MC or various doses of the test compound (e.g., 30, 50, or 100 mg / kg BID). The animals were monitored daily, and tumor volumes were measured twice weekly in two dimensions using calipers, and the volume was calculated using the formula: V = 0.5(a × b 2 ) to mm 3 (where a and b are the long and short diameters of the tumor, respectively). Partial regression (PR) was defined as a tumor volume less than 50% of the initial tumor volume on day 1 of administration in three consecutive measurements, and complete regression (CR) was defined as a tumor volume less than 14 mm in three consecutive measurements. 3 Tumor growth inhibition (TGI) was defined as a tumor volume less than 100 mg / kg. Data are presented as mean tumor volume ± standard error of the mean (SEM). Tumor growth inhibition (TGI) is calculated using the following formula:

number

[0670] PD Research in SW620:

[0671] 5 x 10 cells per 200 μL of PBS / Matrigel were injected into the right flank of female NOD / SCID mice. 6 SW620 cells were subcutaneously transplanted. After inoculation, the tumor size was approximately 350-450 mm. 3Once the average volume reached 100 mg / kg, mice were randomized into treatment groups. Randomized mice were orally administered a single dose of 0.5% MC vehicle or various doses (e.g., 30, 50, or 100 mg / kg) of test compound. Plasma was collected at 0.5, 2, 4, and 8 hours, and tumors were harvested 4 and 8 hours after administration to determine exposure levels. Tumor fragments were snap-frozen in homogenization tubes using liquid nitrogen and homogenized with T-PER Tissue Protein Extraction Buffer freshly supplemented with protease and phosphatase inhibitors before use. Tumor lysates were then analyzed for ERK1 / 2 phosphorylation.

[0672] Efficacy studies in SW620:

[0673] 5 x 10 cells per 200 μL of PBS / Matrigel were injected into the right flank of female NOD / SCID mice. 6 SW620 cells were subcutaneously transplanted. After inoculation, the tumor size was approximately 150-250 mm. 3 Once the average tumor volume reached 100 mg / kg, the mice were randomized into treatment groups. Randomized mice were orally administered a vehicle consisting of 0.5% MC or various doses of the test compound (e.g., 30, 50, or 100 mg / kg BID). The animals were monitored daily, and tumor volumes were measured twice weekly in two dimensions using calipers, and the volume was calculated using the formula: V = 0.5(a × b 2 ) to mm 3 (where a and b are the long and short diameters of the tumor, respectively). Partial regression (PR) was defined as a tumor volume less than 50% of the initial tumor volume on day 1 of administration in three consecutive measurements, and complete regression (CR) was defined as a tumor volume less than 14 mm in three consecutive measurements. 3 Tumor growth inhibition (TGI) was defined as a tumor volume less than 100 mg / kg. Data are presented as mean tumor volume ± standard error of the mean (SEM). Tumor growth inhibition (TGI) is calculated using the following formula:

number

[0674] PD research at RKN:

[0675] 5 x 10 cells per 200 μL of PBS / Matrigel were injected into the right flank of female NOD / SCID mice. 6 RKN cells were subcutaneously transplanted. After inoculation, the tumor size was approximately 350-450 mm. 3 Once the average volume reached 100 mg / kg, mice were randomized into treatment groups. Randomized mice were orally administered a single dose of 0.5% MC vehicle or various doses (e.g., 30, 50, or 100 mg / kg) of test compound. Plasma was collected at 0.5, 2, 4, and 8 hours, and tumors were harvested 4 and 8 hours after administration to determine exposure levels. Tumor fragments were snap-frozen in homogenization tubes using liquid nitrogen and homogenized with T-PER Tissue Protein Extraction Buffer freshly supplemented with protease and phosphatase inhibitors before use. Tumor lysates were then analyzed for ERK1 / 2 phosphorylation.

[0676] Efficacy studies in SW620:

[0677] 5 x 10 cells per 200 μL of PBS / Matrigel were injected into the right flank of female NOD / SCID mice. 6 RKN cells were subcutaneously transplanted. After inoculation, the tumor size was approximately 150-250 mm. 3 Once the average tumor volume reached 100 mg / kg, the mice were randomized into treatment groups. Randomized mice were orally administered a vehicle consisting of 0.5% MC or various doses of the test compound (e.g., 30, 50, or 100 mg / kg BID). The animals were monitored daily, and tumor volumes were measured twice weekly in two dimensions using calipers, and the volume was calculated using the formula: V = 0.5(a × b 2 ) to mm 3 (where a and b are the long and short diameters of the tumor, respectively). Partial regression (PR) was defined as a tumor volume less than 50% of the initial tumor volume on day 1 of administration in three consecutive measurements, and complete regression (CR) was defined as a tumor volume less than 14 mm in three consecutive measurements. 3Tumor growth inhibition (TGI) was defined as a tumor volume less than 100 mg / kg. Data are presented as mean tumor volume ± standard error of the mean (SEM). Tumor growth inhibition (TGI) is calculated using the following formula:

number

[0678] PD research with AsPC-1:

[0679] 3 x 10 cells per 200 μL of PBS / Matrigel were injected into the right flank of female BALB / c nude mice. 6 AsPC-1 cells were subcutaneously implanted. After inoculation, the tumor size was approximately 350–450 mm. 3 Once the average volume reached 100 mg / kg, mice were randomized into treatment groups. Randomized mice were orally administered a single dose of 0.5% MC vehicle or various doses (e.g., 30, 50, or 100 mg / kg) of test compound. Plasma was collected at 0.5, 2, 4, and 8 hours, and tumors were harvested 4 and 8 hours after administration to determine exposure levels. Tumor fragments were snap-frozen in homogenization tubes using liquid nitrogen and homogenized with T-PER Tissue Protein Extraction Buffer freshly supplemented with protease and phosphatase inhibitors before use. Tumor lysates were then analyzed for ERK1 / 2 phosphorylation.

[0680] Efficacy study in AsC-1:

[0681] 3 x 10 cells per 200 μL of PBS / Matrigel were injected into the right flank of female BALB / c nude mice. 6 AsPC-1 cells were subcutaneously implanted. After inoculation, the tumor size was approximately 150-250 mm. 3 Once the average tumor volume reached 100 mg / kg, the mice were randomized into treatment groups. Randomized mice were orally administered a vehicle consisting of 0.5% MC or various doses of the test compound (e.g., 30, 50, or 100 mg / kg BID). The animals were monitored daily, and tumor volumes were measured twice weekly in two dimensions using calipers, and the volume was calculated using the formula: V = 0.5(a × b2 ) to mm 3 (where a and b are the long and short diameters of the tumor, respectively). Partial regression (PR) was defined as a tumor volume less than 50% of the initial tumor volume on day 1 of administration in three consecutive measurements, and complete regression (CR) was defined as a tumor volume less than 14 mm in three consecutive measurements. 3 Tumor growth inhibition (TGI) was defined as a tumor volume less than 100 mg / kg. Data are presented as mean tumor volume ± standard error of the mean (SEM). Tumor growth inhibition (TGI) is calculated using the following formula:

number

[0682] hERG assay

[0683] hERG (human ether-a-go-go related gene) is a rapidly activating potassium channel (I) involved in the repolarization of cardiac action potentials. Kr Blockade of the hERG channel can cause QT interval prolongation on the electrocardiogram, known as long QT syndrome. Drug-induced delay in ventricular repolarization can sometimes lead to torsional apical ventricular tachycardia, a potentially fatal arrhythmia. Approximately 25-40% of lead drug compounds exhibit varying degrees of hERG-dependent potential risk, and many drugs are withdrawn from the market due to the risk of QT interval prolongation.

[0684] Prior to testing hERG currents, the blank control is diluted with an appropriate volume of extracellular solution to make a working control solution. The positive control and test article stock solutions are removed from -20°C, thawed, and diluted with an appropriate volume of extracellular solution to make a working solution.

[0685] The highest test substance working solution should be diluted from a stock solution into the extracellular solution, or the stock solution should first be diluted with DMSO. For other test concentrations of the test substance, serial dilutions should be made using DMSO and then prepared into working solutions in the extracellular solution. The DMSO concentration in the final working solution is 0.3%. Specific preparation information should be recorded on the Compound Working Solution Preparation Form. Finally, all test substance working solutions should be sonicated for 20 minutes before performing patch clamp experiments.

[0686] The blank control (DMSO) stock solution is maintained at room temperature. The blank control working solution is prepared on the day of the experiment and maintained at room temperature. The positive control stock solution and test article stock solutions are maintained at -20°C. The positive control and test article working solutions are prepared on the day of the experiment and maintained at room temperature.

[0687] The test substance concentrations were automatically set to 30, 10, 3, 1, and 0.3 μM. The blank control was 0.3% DMSO, and the positive control (cisapride) concentrations were 1000, 100, 10, 1, and 0.1 nM.

[0688] An automated patch clamp system, QPatch 48X (Sophion), is used for electrophysiological recordings in this experiment.

[0689] The prepared cells are placed in the centrifuge on the Qpatch work surface, and multiple centrifugations / suspensions are used to wash the cells and exchange the cell culture medium for extracellular solution. The MTP-96 plate is removed and placed in the MTP source position. The QPlate chip is removed and placed in the Qplate source position. A barcode reader scans the barcodes on the MTP-96 board and QPlate chip, and the gripper arm grips them to the measurement position. Intracellular and extracellular solutions are added from the saline reservoir to the intracellular saline well, cell well, and compound well of the QPlate chip. For measurement, all measurement positions on the QPlate undergo an initial quality control. This process involves aspirating the cell suspension from the centrifuge's cell container, placing the cells over the chip hole with the pressure regulator, establishing a high-resistance seal, and configuring the whole-cell recording mode. Once a stable baseline control current is obtained, test substances are applied to the cells in concentration order by sequential aspiration from the MTP-96 plate. hERG currents are recorded using whole-cell patch clamp technique at a holding potential of -80 mV, followed by a 0.5-second depolarization to -50 mV to examine leak currents. The potential is then depolarized to 30 mV for 2.5 seconds. Peak tail currents are elicited by a 4-second repolarization pulse to -50 mV. This protocol is repeated at 10-second intervals to observe the effects of test substances on hERG tail currents. Data are collected by the QPatch screening station and stored in the QPatch database server.

[0690] In the experiment, each drug concentration is applied twice with a recording period of at least 5 minutes. Control and test solutions are applied to the cells sequentially from low to high concentrations. The current of each cell detected with compound-free extracellular solution serves as its own blank control.

[0691] I C 50 Calculate IC values ​​and fit the dose-response curve using the nonlinear regression equation above (where IC 50 IC is the 50% inhibitory concentration.50 Calculations and curve fitting are performed using GraphPad Prism software.

[0692] Activity table Each of the compounds in Tables 2 and 3 was tested in, and found to exhibit activity in, one or more of the biochemical assays provided herein.

[0693] [Table 2]

[0694] [Table 3]

[0695] As demonstrated by the data in Tables 2-3, the inventors have surprisingly and unexpectedly discovered that representative compounds in Tables 2-3 modulate or inhibit the activity of KRAS G12D and / or G12V.

[0696] A number of references have been cited, the disclosures of which are incorporated herein by reference in their entireties.

Claims

1. Formula (I): 【Chemical 1】 or a pharmaceutically acceptable salt, tautomer, isotopic substitution, stereoisomer, enantiomer, atropisomer, or prodrug thereof, having During the ceremony, Ring A is unsubstituted or substituted aryl, or unsubstituted or substituted heteroaryl; Ring B is unsubstituted or substituted cycloalkyl or unsubstituted or substituted heterocyclyl; X is N or C-R ８ and R ０ each independently represents H, halogen, amino, —CN, —OH, unsubstituted or substituted C １～４ Alkyl, unsubstituted or substituted C １～４ Alkenyl, unsubstituted or substituted C １～４ Alkynyl, unsubstituted or substituted C １～４ Alkoxy, unsubstituted or substituted C ３～５ Cycloalkyl, 3- to 5-membered unsubstituted or substituted heterocyclyl, unsubstituted or substituted C １～４ alkylamino, carboxy, nitro, thiol, or thioether, or R ０ one or more pairs of groups, together with the atoms to which they are attached, form an unsubstituted or substituted cycloalkyl, unsubstituted or substituted heterocyclyl, unsubstituted or substituted aryl, or unsubstituted or substituted heteroaryl; R ３ａ , R ３ｂ , R ４ａ , R ４ｂ , R ５ , R ６ａ , R ６ｂ , R ７ａ , and R ７ｂ each independently represents H, halogen, unsubstituted or substituted amino, —CN, —OH, unsubstituted or substituted C １～４ Alkyl, unsubstituted or substituted C １～４ Alkoxy, unsubstituted or substituted C ３～５ Cycloalkyl, 3- to 5-membered unsubstituted or substituted heterocyclyl, unsubstituted or substituted C １～４ alkylamino, carboxy, nitro, thiol, or thioether, and optionally R ３ａ and R ３ｂ together with the atom to which they are attached form an unsubstituted or substituted cycloalkyl or an unsubstituted or substituted heterocyclyl, or R ４ａ and R ４ｂ together with the atom to which they are attached form an unsubstituted or substituted cycloalkyl or an unsubstituted or substituted heterocyclyl, or R ６ａ and R ６ｂ together with the atom to which they are attached form an unsubstituted or substituted cycloalkyl or an unsubstituted or substituted heterocyclyl, or R ７ａ and R ７ｂ together with the atom to which they are attached form an unsubstituted or substituted cycloalkyl, or an unsubstituted or substituted heterocyclyl, or optionally R ３ａ and R ４ａ together with the atom to which they are attached form an unsubstituted or substituted cycloalkyl or an unsubstituted or substituted heterocyclyl, or R ４ａ and R ５ together with the atom to which they are attached form an unsubstituted or substituted cycloalkyl or an unsubstituted or substituted heterocyclyl, or R ５ and R ６ａ together with the atom to which they are attached form an unsubstituted or substituted cycloalkyl or an unsubstituted or substituted heterocyclyl, or R ６ａ and R ７ａ together with the atom to which they are attached form an unsubstituted or substituted cycloalkyl, or an unsubstituted or substituted heterocyclyl; R ８ is H, halogen, unsubstituted or substituted C １～４ Alkyl, unsubstituted or substituted C １～４ Alkenyl, unsubstituted or substituted C ３～５ Cycloalkyl, unsubstituted or substituted C １～４ Alkoxyl, unsubstituted or substituted C １～４ Alkyl halides, unsubstituted or substituted C ３～５ Halogenated cycloalkyl, unsubstituted or substituted C １～４ halogenated alkoxyl, CN, OH, or amino; t is 0 or 1; u is 1, 2, 3 or 4; wherein each of m and q is independently an integer between 0 and the maximum number of substituents allowed on rings A and B, respectively, or a pharmaceutically acceptable salt, tautomer, isotopic substitution, stereoisomer, enantiomer, atropisomer, or prodrug thereof.

2. X is N, C—H, C—Cl, or C—CF ３ 2. The compound of claim 1, wherein:

3. Ring A is substituted or unsubstituted phenyl, substituted or unsubstituted pyridinyl, substituted or unsubstituted naphthyl, substituted or unsubstituted benzo[b]thiophenyl, or substituted or unsubstituted benzo[d]thiazolyl; The compound of claim 2, wherein Ring B is substituted or unsubstituted hexahydro-1H-pyrrolidinyl, substituted or unsubstituted pyrrolidinyl, substituted or unsubstituted aminomethylcyclopropyl, substituted or unsubstituted oxetanyl, substituted or unsubstituted tetrahydrofuranyl, substituted or unsubstituted oxabicyclo[2.1.1]hexanyl, or substituted or unsubstituted oxabicyclo[2.2.1]heptanyl.

4. Ring A is 【Chemistry 2】 4. The compound of claim 3, wherein:

5. Ring B is 【Chemistry 3】 wherein R ａ and R ｂ each independently being a substituted or unsubstituted C １～４ Alkyl, substituted or unsubstituted C ３～５ cycloalkyl or R ａ and R ｂ together with the N to which they are attached form a substituted or unsubstituted heterocycle containing N, O, or S; R ｃ is H, halogen, CN, substituted or unsubstituted C １～４ Alkyl, substituted or unsubstituted C ３～５ Cycloalkyl, substituted or unsubstituted C １～４ The compound of claim 4 which is an alkoxyl.

6. The compound has the formula (II): 【Chemistry 4】 6. The compound of claim 5, wherein the compound has the formula:

7. X is N and ring A is 【Chemistry 5】 and ring B is 【Chemistry 6】 wherein R ａ and R ｂ each independently being a substituted or unsubstituted C １～４ Alkyl, substituted or unsubstituted C ３～５ cycloalkyl or R ａ and R ｂ together with the N to which they are attached form a substituted or unsubstituted heterocycle containing N, O, or S; R ｃ is H, halogen, CN, substituted or unsubstituted C １～４ Alkyl, substituted or unsubstituted C ３～５ Cycloalkyl, substituted or unsubstituted C １～４ is alkoxyl, and R ３ａ , R ３ｂ , R ５ , R ７ａ , and R ７ｂ are each independently H or methyl, and R ６ａ１ , R ６ｂ１ is independently selected from H, halogen, methyl, —CN, —OH, substituted or unsubstituted amino.

8. X is N and ring A is 【Chemistry 7】 and ring B is 【Chemistry 8】 wherein R ａ and R ｂ each independently being a substituted or unsubstituted C １～４ Alkyl, substituted or unsubstituted C ３～５ cycloalkyl or R ａ and R ｂ together with the N to which they are attached form a substituted or unsubstituted heterocycle containing N, O, or S, and R ３ａ , R ３ｂ , R ５ , R ７ａ , and R ７ｂ are each independently H or methyl, and R ６ａ１ and R ６ｂ１ together with the atoms to which they are attached form an unsubstituted or substituted heterocyclyl.

9. X is N and ring A is 【Chemistry 9】 and ring B is 【Chemistry 10】 wherein R ａ and R ｂ each independently being a substituted or unsubstituted C １～４ Alkyl, substituted or unsubstituted C ３～５ cycloalkyl or R ａ and R ｂ together with the N to which they are attached form a substituted or unsubstituted heterocycle containing N, O, or S; R ｃ is H, halogen, CN, substituted or unsubstituted C １～４ Alkyl, substituted or unsubstituted C ３～５ Cycloalkyl, substituted or unsubstituted C １～４ is alkoxyl, and R ３ａ , R ３ｂ , R ５ , R ７ａ , and R ７ｂ are each independently H or methyl, and R ６ａ１ , R ６ｂ１ is independently selected from H, halogen, methyl, —CN, —OH, substituted or unsubstituted amino.

10. The compound has the formula (III): 【Chemistry 11】 6. The compound of claim 5, wherein the compound has the formula:

11. X is N and ring A is 【Chemistry 12】 and ring B is 【Chemistry 13】 wherein R ａ and R ｂ each independently being a substituted or unsubstituted C １～４ Alkyl, substituted or unsubstituted C ３～５ cycloalkyl or R ａ and R ｂ together with the N to which they are attached form a substituted or unsubstituted heterocycle containing N, O, or S; R ｃ is H, halogen, CN, substituted or unsubstituted C １～４ Alkyl, substituted or unsubstituted C ３～５ Cycloalkyl, substituted or unsubstituted C １～４ is alkoxyl, and R ３ａ , R ３ｂ , R ５ , R ６ａ１ , R ６ｂ１ , R ６ａ２ , R ６ｂ２ , R ７ａ , and R ７ｂ 11. The compound of claim 10, wherein each of is independently H, halogen, OH, CN, substituted or unsubstituted amino, or methyl.

12. X is N and ring A is 【Chemistry 14】 and ring B is 【Chemistry 15】 wherein R ａ and R ｂ each independently being a substituted or unsubstituted C １～４ Alkyl, substituted or unsubstituted C ３～５ cycloalkyl or R ａ and R ｂ together with the N to which they are attached form a substituted or unsubstituted heterocycle containing N, O, or S; R ｃ is H, halogen, CN, substituted or unsubstituted C １～４ Alkyl, substituted or unsubstituted C ３～５ Cycloalkyl, substituted or unsubstituted C １～４ is alkoxyl, and R ３ａ , R ３ｂ , R ５ , R ６ａ１ , R ６ｂ１ , R ６ａ２ , R ６ｂ２ , R ７ａ , and R ７ｂ each independently is H, halogen, OH, CN, substituted or unsubstituted amino, or methyl, or optionally, R ３ａ and R ５ together with the atom to which they are attached form an unsubstituted or substituted cycloalkyl or an unsubstituted or substituted heterocyclyl, or R ６ａ１ and R ５ together with the atom to which they are attached form an unsubstituted or substituted cycloalkyl or an unsubstituted or substituted heterocyclyl, or R ６ａ１ and R ６ａ２ together with the atom to which they are attached form an unsubstituted or substituted cycloalkyl or an unsubstituted or substituted heterocyclyl, or R ６ａ２ and R ７ａ together with the atoms to which they are attached form an unsubstituted or substituted cycloalkyl or an unsubstituted or substituted heterocyclyl.

13. X is C—H and ring A is 【Chemistry 16】 and ring B is 【Chemistry 17】 wherein R ａ and R ｂ each independently being a substituted or unsubstituted C １～４ Alkyl, substituted or unsubstituted C ３～５ cycloalkyl or R ａ and R ｂ together with the N to which they are attached form a substituted or unsubstituted heterocycle containing N, O, or S; R ｃ is H, halogen, CN, substituted or unsubstituted C １～４ Alkyl, substituted or unsubstituted C ３～５ Cycloalkyl, substituted or unsubstituted C １～４ is alkoxyl, and R ３ａ , R ３ｂ , R ５ , R ６ａ１ , R ６ｂ１ , R ６ａ２ , R ６ｂ２ , R ７ａ , and R ７ｂ 11. The compound of claim 10, wherein each of is independently H, halogen, OH, CN, substituted or unsubstituted amino, or methyl.

14. X is C—H and ring A is 【Chemistry 18】 and ring B is 【Chemistry 19】 wherein R ａ and R ｂ each independently being a substituted or unsubstituted C １～４ Alkyl, substituted or unsubstituted C ３～５ cycloalkyl or R ａ and R ｂ together with the N to which they are attached form a substituted or unsubstituted heterocycle containing N, O, or S; R ｃ is H, halogen, CN, substituted or unsubstituted C １～４ Alkyl, substituted or unsubstituted C ３～５ Cycloalkyl, substituted or unsubstituted C １～４ is alkoxyl, and R ３ａ , R ３ｂ , R ５ , R ６ａ１ , R ６ｂ１ , R ６ａ２ , R ６ｂ２ , R ７ａ , and R ７ｂ 11. The compound of claim 10, wherein each of is independently H, halogen, OH, CN, substituted or unsubstituted amino, or methyl.

15. X is C—F and ring A is 【Chemistry 20】 and ring B is 【Chemical 21】 wherein R ａ and R ｂ each independently being a substituted or unsubstituted C １～４ Alkyl, substituted or unsubstituted C ３～５ cycloalkyl or R ａ and R ｂ together with the N to which they are attached form a substituted or unsubstituted heterocycle containing N, O, or S; R ３ａ , R ３ｂ , R ５ , R ６ａ１ , R ６ｂ１ , R ６ａ２ , R ６ｂ２ , R ７ａ , and R ７ｂ 11. The compound of claim 10, wherein each of is independently H, halogen, OH, CN, substituted or unsubstituted amino, or methyl.

16. X is C—Cl, and ring A is 【Chemical 22】 and ring B is 【Chemical 23】 wherein R ａ and R ｂ each independently being a substituted or unsubstituted C １～４ Alkyl, substituted or unsubstituted C ３～５ cycloalkyl or R ａ and R ｂ together with the N to which they are attached form a substituted or unsubstituted heterocycle containing N, O, or S; R ３ａ , R ３ｂ , R ５ , R ６ａ１ , R ６ｂ１ , R ６ａ２ , R ６ｂ２ , R ７ａ , and R ７ｂ 11. The compound of claim 10, wherein each of is independently H, halogen, OH, CN, substituted or unsubstituted amino, or methyl.

17. X is C—Cl, and ring A is 【Chemistry 24】 and ring B is 【Chemistry 25】 wherein R ａ and R ｂ each independently being a substituted or unsubstituted C １～４ Alkyl, substituted or unsubstituted C ３～５ cycloalkyl or R ａ and R ｂ together with the N to which they are attached form a substituted or unsubstituted heterocycle containing N, O, or S; R ３ａ , R ３ｂ , R ５ , R ６ａ１ , R ６ｂ１ , R ６ａ２ , R ６ｂ２ , R ７ａ , and R ７ｂ 11. The compound of claim 10, wherein each of is independently H, halogen, OH, CN, substituted or unsubstituted amino, or methyl.

18. X is C-CF ３ and ring A is 【Chemical Formula 26】 and ring B is 【Chemical 27】 wherein R ａ and R ｂ each independently being a substituted or unsubstituted C １～４ Alkyl, substituted or unsubstituted C ３～５ cycloalkyl or R ａ and R ｂ together with the N to which they are attached form a substituted or unsubstituted heterocycle containing N, O, or S; R ｃ is H, halogen, CN, substituted or unsubstituted C １～４ Alkyl, substituted or unsubstituted C ３～５ Cycloalkyl, substituted or unsubstituted C １～４ is alkoxyl, R ３ａ , R ３ｂ , R ５ , R ６ａ１ , R ６ｂ１ , R ６ａ２ , R ６ｂ２ , R ７ａ , and R ７ｂ 11. The compound of claim 10, wherein each of is independently H, halogen, OH, CN, substituted or unsubstituted amino, or methyl.

19. The compound has the formula (IV): 【Chemical Formula 28】 6. The compound of claim 5, wherein the compound has the formula:

20. X is N and ring A is 【Chemical 29】 and ring B is 【Chemistry 30】 wherein R ａ and R ｂ each independently being a substituted or unsubstituted C １～４ Alkyl, substituted or unsubstituted C ３～５ cycloalkyl or R ａ and R ｂ together with the N to which they are attached form a substituted or unsubstituted heterocycle containing N, O, or S; R ｃ is H, halogen, CN, substituted or unsubstituted C １～４ Alkyl, substituted or unsubstituted C ３～５ Cycloalkyl, substituted or unsubstituted C １～４ is alkoxyl, R ３ａ , R ３ｂ , R ５ , R ６ａ１ , R ６ｂ１ , R ６ａ２ , R ６ｂ２ , R ６ａ３ , R ６ｂ３ , R ７ａ , and R ７ｂ 20. The compound of claim 19, wherein each of is independently H, halogen, OH, CN, substituted or unsubstituted amino, hydroxylmethyl, or methyl.

21. X is N and ring A is 【Chemical 31】 and ring B is 【Chemical 32】 wherein R ａ and R ｂ each independently being a substituted or unsubstituted C １～４ Alkyl, substituted or unsubstituted C ３～５ cycloalkyl or R ａ and R ｂ together with the N to which they are attached form a substituted or unsubstituted heterocycle containing N, O, or S; R ｃ is H, halogen, CN, substituted or unsubstituted C １～４ Alkyl, substituted or unsubstituted C ３～５ Cycloalkyl, substituted or unsubstituted C １～４ is alkoxyl, R ３ａ , R ３ｂ , R ５ , R ６ａ１ , R ６ｂ１ , R ６ａ２ , R ６ｂ２ , R ６ａ３ , R ６ｂ３ , R ７ａ , and R ７ｂ 20. The compound of claim 19, wherein each of is independently H, halogen, OH, CN, substituted or unsubstituted amino, or methyl.

22. X is C—Cl, and ring A is 【Chemical 33】 and ring B is 【Chemical 34】 wherein R ａ and R ｂ each independently being a substituted or unsubstituted C １～４ Alkyl, substituted or unsubstituted C ３～５ cycloalkyl or R ａ and R ｂ together with the N to which they are attached form a substituted or unsubstituted heterocycle containing N, O, or S; R ｃ is H, halogen, CN, substituted or unsubstituted C １～４ Alkyl, substituted or unsubstituted C ３～５ Cycloalkyl, substituted or unsubstituted C １～４ is alkoxyl, R ３ａ , R ３ｂ , R ５ , R ６ａ１ , R ６ｂ１ , R ６ａ２ , R ６ｂ２ , R ６ａ３ , R ６ｂ３ , R ７ａ , and R ７ｂ 20. The compound of claim 19, wherein each of is independently H, halogen, OH, CN, substituted or unsubstituted amino, or methyl.

23. X is C—Cl, and ring A is 【Chemical 35】 and ring B is 【Chemical 36】 wherein R ａ and R ｂ each independently being a substituted or unsubstituted C １～４ Alkyl, substituted or unsubstituted C ３～５ cycloalkyl or R ａ and R ｂ together with the N to which they are attached form a substituted or unsubstituted heterocycle containing N, O, or S; R ３ａ , R ３ｂ , R ５ , R ６ａ１ , R ６ｂ１ , R ６ａ２ , R ６ｂ２ , R ６ａ３ , R ６ｂ３ , R ７ａ , and R ７ｂ 20. The compound of claim 19, wherein each of is independently H, halogen, OH, CN, substituted or unsubstituted amino, or methyl.

24. X is C—Cl, and ring A is 【Chemical 37】 and ring B is 【Chemical Formula 38】 wherein R ｃ is H, halogen, CN, substituted or unsubstituted C １～４ Alkyl, substituted or unsubstituted C ３～５ Cycloalkyl, substituted or unsubstituted C １～４ is alkoxyl, R ３ａ , R ３ｂ , R ５ , R ６ａ１ , R ６ｂ１ , R ６ａ２ , R ６ｂ２ , R ６ａ３ , R ６ｂ３ , R ７ａ , and R ７ｂ 20. The compound of claim 19, wherein each of is independently H, halogen, OH, CN, substituted or unsubstituted amino, or methyl.

25. The compound has the formula (V): 【Chemical Formula 39】 6. The compound of claim 5, wherein the compound has the formula:

26. X is N and ring A is 【Chemistry 40】 and ring B is 【Chemistry 41】 wherein R ａ and R ｂ each independently being a substituted or unsubstituted C １～４ Alkyl, substituted or unsubstituted C ３～５ cycloalkyl or R ａ and R ｂ together with the N to which they are attached form a substituted or unsubstituted heterocycle containing N, O, or S; R ｃ is H, halogen, CN, substituted or unsubstituted C １～４ Alkyl, substituted or unsubstituted C ３～５ Cycloalkyl, substituted or unsubstituted C １～４ is alkoxyl, R ３ａ , R ３ｂ , R ５ , R ６ａ１ , R ６ｂ１ , R ６ａ２ , R ６ｂ２ , R ６ａ３ , R ６ｂ３ , R ６ａ４ , R ６ｂ４ , R ７ａ , and R ７ｂ 26. The compound of claim 25, wherein each of is independently H, halogen, OH, CN, substituted or unsubstituted amino, or methyl.

27. The compound has the formula (VI): 【Chemistry 42】 6. The compound of claim 5, wherein the compound has the formula:

28. X is N and ring A is 【Chemistry 43】 and ring B is 【Chemical 44】 wherein R ａ and R ｂ each independently being a substituted or unsubstituted C １～４ Alkyl, substituted or unsubstituted C ３～５ cycloalkyl or R ａ and R ｂ together with the N to which they are attached form a substituted or unsubstituted heterocycle containing N, O, or S; R ｃ is H, halogen, CN, substituted or unsubstituted C １～４ Alkyl, substituted or unsubstituted C ３～５ Cycloalkyl, substituted or unsubstituted C １～４ is alkoxyl, R ３ａ , R ３ｂ , R ４ａ , R ４ｂ , R ５ , R ６ａ１ , R ６ｂ１ , R ７ａ , and R ７ｂ 28. The compound of claim 27, wherein each of is independently H, halogen, OH, CN, substituted or unsubstituted amino, or methyl.

29. The compound has the formula (VII): 【Chemistry 45】 6. The compound of claim 5, wherein the compound has the formula:

30. X is N and ring A is 【Chemistry 46】 and ring B is 【Chemistry 47】 wherein R ｃ is H, halogen, CN, substituted or unsubstituted C １～４ Alkyl, substituted or unsubstituted C ３～５ Cycloalkyl, substituted or unsubstituted C １～４ is alkoxyl, R ３ａ , R ３ｂ , R ４ａ , R ４ｂ , R ５ , R ６ａ１ , R ６ｂ１ , R ６ａ２ , R ６ｂ２ , R ７ａ , and R ７ｂ 30. The compound of claim 29, wherein each of is independently H, halogen, OH, CN, substituted or unsubstituted amino, or methyl.

31. X is N and ring A is 【Chemistry 48】 and ring B is 【Chemistry 49】 wherein R ａ and R ｂ each independently being a substituted or unsubstituted C １～４ Alkyl, substituted or unsubstituted C ３～５ cycloalkyl or R ａ and R ｂ together with the N to which they are attached form a substituted or unsubstituted heterocycle containing N, O, or S; R ３ａ , R ３ｂ , R ４ａ , R ４ｂ , R ５ , R ６ａ１ , R ６ｂ１ , R ６ａ２ , R ６ｂ２ , R ７ａ , and R ７ｂ 30. The compound of claim 29, wherein each of is independently H, halogen, OH, CN, substituted or unsubstituted amino, or methyl.

32. X is N and ring A is 【Chemistry 50】 and ring B is 【Chemistry 51】 wherein R ａ and R ｂ each independently being a substituted or unsubstituted C １～４ Alkyl, substituted or unsubstituted C ３～５ cycloalkyl or R ａ and R ｂ together with the N to which they are attached form a substituted or unsubstituted heterocycle containing N, O, or S; R ３ａ , R ３ｂ , R ４ａ , R ４ｂ , R ５ , R ６ａ１ , R ６ｂ１ , R ６ａ２ , R ６ｂ２ , R ７ａ , and R ７ｂ 30. The compound of claim 29, wherein each of is independently H, halogen, OH, CN, substituted or unsubstituted amino, or methyl.

33. X is C—H and ring A is 【Chemistry 52】 and ring B is 【Chemistry 53】 wherein R ａ and R ｂ each independently being a substituted or unsubstituted C １～４ Alkyl, substituted or unsubstituted C ３～５ cycloalkyl or R ａ and R ｂ together with the N to which they are attached form a substituted or unsubstituted heterocycle containing N, O, or S; R ３ａ , R ３ｂ , R ４ａ , R ４ｂ , R ５ , R ６ａ１ , R ６ｂ１ , R ６ａ２ , R ６ｂ２ , R ７ａ , and R ７ｂ 30. The compound of claim 29, wherein each of is independently H, halogen, OH, CN, substituted or unsubstituted amino, or methyl.

34. X is C—H and ring A is 【Chemical 54】 and ring B is 【Chemistry 55】 wherein R ａ and R ｂ each independently being a substituted or unsubstituted C １～４ Alkyl, substituted or unsubstituted C ３～５ cycloalkyl or R ａ and R ｂ together with the N to which they are attached form a substituted or unsubstituted heterocycle containing N, O, or S; R ３ａ , R ３ｂ , R ４ａ , R ４ b, R ５ , R ６ａ１ , R ６ｂ１ , R ６ａ２ , R ６ｂ２ , R ７ａ , and R ７ｂ 30. The compound of claim 29, wherein each of is independently H, halogen, OH, CN, substituted or unsubstituted amino, or methyl.

35. X is C—Cl, and ring A is 【Chemical 56】 and ring B is 【Chemical Formula 57】 wherein R ｃ is H, halogen, CN, substituted or unsubstituted C １～４ Alkyl, substituted or unsubstituted C ３～５ Cycloalkyl, substituted or unsubstituted C １～４ is alkoxyl, R ３ａ , R ３ｂ , R ４ａ , R ４ｂ , R ５ , R ６ａ１ , R ６ｂ１ , R ６ａ２ , R ６ｂ２ , R ７ａ , and R ７ｂ 30. The compound of claim 29, wherein each of is independently H, halogen, OH, CN, substituted or unsubstituted amino, or methyl.

36. X is C—Cl, and ring A is 【Chemistry 58】 and ring B is 【Chemical Formula 59】 wherein R ａ and R ｂ each independently being a substituted or unsubstituted C １～４ Alkyl, substituted or unsubstituted C ３～５ cycloalkyl or R ａ and R ｂ together with the N to which they are attached form a substituted or unsubstituted heterocycle containing N, O, or S; R ３ａ , R ３ｂ , R ４ａ , R ４ｂ , R ５ , R ６ａ１ , R ６ｂ１ , R ６ａ２ , R ６ｂ２ , R ７ａ , and R ７ｂ 30. The compound of claim 29, wherein each of is independently H, halogen, OH, CN, substituted or unsubstituted amino, or methyl.

37. X is C-CF ３ and ring A is 【Chemistry 60】 and ring B is 【Hua 61】 wherein R ａ and R ｂ each independently being a substituted or unsubstituted C １～４ Alkyl, substituted or unsubstituted C ３～５ cycloalkyl or R ａ and R ｂ together with the N to which they are attached form a substituted or unsubstituted heterocycle containing N, O, or S; R ｃ is H, halogen, CN, substituted or unsubstituted C １～４ Alkyl, substituted or unsubstituted C ３～５ Cycloalkyl, substituted or unsubstituted C １～４ is alkoxyl, R ３ａ , R ３ｂ , R ４ａ , R ４ｂ , R ５ , R ６ａ１ , R ６ｂ１ , R ６ａ２ , R ６ｂ２ , R ７ａ , and R ７ｂ 30. The compound of claim 29, wherein each of is independently H, halogen, OH, CN, substituted or unsubstituted amino, or methyl.

38. The compound has the formula (VIII): 【Hua 62】 6. The compound of claim 5, wherein the compound has the formula:

39. X is N and ring A is 【Chemistry 63】 and ring B is 【Hua 64】 wherein R ａ and R ｂ each independently being a substituted or unsubstituted C １～４ Alkyl, substituted or unsubstituted C ３～５ cycloalkyl or R ａ and R ｂ together with the N to which they are attached form a substituted or unsubstituted heterocycle containing N, O, or S; R ｃ is H, halogen, CN, substituted or unsubstituted C １～４ Alkyl, substituted or unsubstituted C ３～５ Cycloalkyl, substituted or unsubstituted C １～４ is alkoxyl, R ３ａ , R ３ｂ , R ４ａ , R ４ｂ , R ５ , R ６ａ１ , R ６ｂ１ , R ６ａ２ , R ６ｂ２ , R ６ａ３ , R ６ｂ３ , R ７ａ , and R ７ｂ 39. The compound of claim 38, wherein each of is independently H, halogen, OH, CN, substituted or unsubstituted amino, or methyl.

40. X is C—H and ring A is 【Chemistry 65】 and ring B is 【Hua 66】 wherein R ａ and R ｂ each independently being a substituted or unsubstituted C １～４ Alkyl, substituted or unsubstituted C ３～５ cycloalkyl or R ａ and R ｂ together with the N to which they are attached form a substituted or unsubstituted heterocycle containing N, O, or S; R ｃ is H, halogen, CN, substituted or unsubstituted C １～４ Alkyl, substituted or unsubstituted C ３～５ Cycloalkyl, substituted or unsubstituted C １～４ is alkoxyl, R ３ａ , R ３ｂ , R ４ａ , R ４ｂ , R ５ , R ６ａ１ , R ６ｂ１ , R ６ａ２ , R ６ｂ２ , R ６ａ３ , R ６ｂ３ , R ７ａ , and R ７ｂ 39. The compound of claim 38, wherein each of is independently H, halogen, OH, CN, substituted or unsubstituted amino, or methyl.

41. The compound has the formula (IX): 【Chemical Formula 67】 6. The compound of claim 5, wherein the compound has the formula:

42. X is N and ring A is 【Chemistry 68】 and ring B is 【Chemical Formula 69】 wherein R ａ and R ｂ each independently being a substituted or unsubstituted C １～４ Alkyl, substituted or unsubstituted C ３～５ cycloalkyl or R ａ and R ｂ together with the N to which they are attached form a substituted or unsubstituted heterocycle containing N, O, or S; R ｃ is H, halogen, CN, substituted or unsubstituted C １～４ Alkyl, substituted or unsubstituted C ３～５ Cycloalkyl, substituted or unsubstituted C １～４ is alkoxyl, R ３ａ , R ３ｂ , R ４ａ , R ４ｂ , R ５ , R ６ａ１ , R ６ｂ１ , R ６ａ２ , R ６ｂ２ , R ６ａ３ , R ６ｂ３ , R ６ａ４ , R ６ｂ４ , R ７ａ , and R ７ｂ 42. The compound of claim 41, wherein each of is independently H, halogen, OH, CN, substituted or unsubstituted amino, or methyl.

43. The compound has the formula (Ia): 【Chemistry 70】 or a pharmaceutically acceptable salt, tautomer, stereoisomer, enantiomer, atropisomer, isotopically substituted derivative, or prodrug thereof; During the ceremony, Ring C is unsubstituted or substituted C ３～６ cycloalkyl, or 3- to 6-membered unsubstituted or substituted heterocyclyl; each of u and v is independently an integer; The compound of claim 5 , wherein the sum of u and v is 1, 2, 3, or 4.

44. 44. The compound of any one of claims 1 to 43, wherein the compound is selected from Tables 1 to 3.

45. 45. A pharmaceutical composition comprising an effective amount of a compound of any one of claims 1 to 44, or a pharmaceutically acceptable salt, tautomer, isotopic substitution, stereoisomer, enantiomer, atropisomer, or prodrug thereof, and a pharmaceutically acceptable carrier, excipient, or vehicle.

46. 45. A method for inhibiting the activity of a KRAS mutant protein or KRAS amplification in a cell, comprising contacting the cell with an effective amount of a compound of any one of claims 1 to 44, or a pharmaceutically acceptable salt, tautomer, isotopic substitute, stereoisomer, enantiomer, atropisomer, or prodrug thereof, wherein optionally the KRAS mutant protein is a KRAS G12D and / or G12V mutant protein.

47. 45. A method for the treatment or prevention of cancer, comprising administering to a subject in need thereof an effective amount of a compound of any one of claims 1 to 44, or a pharmaceutically acceptable salt, tautomer, isotopic substitute, stereoisomer, enantiomer, atropisomer, or prodrug thereof, optionally wherein the cancer is mediated by a KRAS mutation, preferably a KRAS G12D and / or G12V mutation.

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