Device for collecting a biological sample

Abstract

To efficiently collect a biological sample.SOLUTION: A device for collecting a biological sample from a living body, in which a first adhesive layer, a paper base material, and a second adhesive layer are laminated from the side of a surface to be attached to the living body, the paper base material has a hydrophobic portion and a non-hydrophobic portion, the hydrophobic portion surrounds the non-hydrophobic portion, and the first adhesive layer has a collection section for collecting the biological sample at a position overlapping a part of the non-hydrophobic portion of the paper base material in a plan view.SELECTED DRAWING: Figure 2A

Description

[Technical Field]

[0001] The present invention relates to a device for collecting biological samples. [Background technology]

[0002] Conventionally, a liquid collection device has been known that has a capillary-shaped micro-channel with a first opening at one end of the micro-channel and a second opening at the other end, and that collects a biological sample. [Prior art documents] [Patent documents]

[0003] [Patent Document 1] Patent Publication No. 2021-1762 Summary of the Invention [Problem to be solved by the invention]

[0004] In the conventional liquid sampling device described above, openings are formed at both ends of the flow path, which may cause the liquid stored in the flow path to leak out. For this reason, when the amount of liquid to be sampled from a living body is small, the conventional liquid sampling device has difficulty in sampling the liquid, and is unable to efficiently sample a living body sample.

[0005] The disclosed technology aims to efficiently collect biological samples. [Means for solving the problem]

[0006] The disclosed technology is a device for collecting biological samples from a living organism, which comprises a first adhesive layer, a paper base material, and a second adhesive layer laminated from the side that is attached to the living organism, the paper base material having a hydrophobic portion and a non-hydrophobic portion, the hydrophobic portion surrounding the non-hydrophobic portion, and the first adhesive layer having a collection portion for collecting the biological sample at a position that overlaps with part of the non-hydrophobic portion of the paper base material in a planar view. [Effects of the Invention]

[0007] Biological samples can be collected efficiently. [Brief explanation of the drawings]

[0008] [Figure 1] FIG. 1 is a top view of the device. [Figure 2A] FIG. 2 is a diagram illustrating a configuration of a device according to the first embodiment. [Figure 2B] FIG. 2 is a cross-sectional view taken along line AA of the device according to the first embodiment. [Figure 3A] FIG. 10 is a diagram illustrating another configuration of the device according to the first embodiment. [Figure 3B] FIG. 2 is another cross-sectional view taken along line AA of the device of the first embodiment. [Figure 4A] FIG. 10 is a diagram illustrating a configuration of a device according to a second embodiment. [Figure 4B] FIG. 2 is a cross-sectional view taken along line AA of the device according to the second embodiment. [Figure 5A] FIG. 10 is a diagram illustrating another configuration of the device according to the second embodiment. [Figure 5B] FIG. 10 is another cross-sectional view taken along line AA of the device of the second embodiment. [Figure 6A] FIG. 10 is a diagram illustrating a configuration of a device according to a second embodiment. [Figure 6B] FIG. 2 is a cross-sectional view taken along line AA of the device according to the second embodiment. [Figure 7A] FIG. 10 is a diagram illustrating another configuration of the device according to the third embodiment. [Figure 7B] FIG. 10 is another cross-sectional view of the device of the third embodiment. [Figure 8A] FIG. 11 is an example of a cross-sectional view taken along line BB of the device according to the third embodiment. [Figure 8B] 10 is another example of a BB cross-sectional view of the device according to the third embodiment. [Figure 9] FIG. 1 illustrates an example of a system configuration of an analysis system. [Figure 10] FIG. 2 illustrates an example of a hardware configuration of an analysis apparatus. [Figure 11]FIG. 2 is a diagram illustrating the functional configuration of the analysis device. [Figure 12] 10 is a flowchart illustrating processing by the analysis device. [Figure 13] FIG. 1 is a first diagram illustrating the structure of a colorimetric sensor. [Figure 14] FIG. 2 is a second diagram illustrating the structure of the colorimetric sensor. [Figure 15] FIG. 3 is a third diagram illustrating the structure of a colorimetric sensor. [Figure 16] FIG. 10 is a first diagram illustrating the shape of a second end portion. [Figure 17] FIG. 10 is a diagram (part 1) showing an example of the shape of the second end portion. [Figure 18] FIG. 10 is a diagram (part 2) showing an example of the shape of the second end portion. [Figure 19] FIG. 10 is a diagram (part 3) showing an example of the shape of the second end portion. [Figure 20] FIG. 10 is a diagram (part 4) showing an example of the shape of the second end portion. DETAILED DESCRIPTION OF THE INVENTION

[0009] (First embodiment) The device of this embodiment will be described below with reference to Figures 1 to 3. Figure 1 is a top view of the device.

[0010] The device 100 of this embodiment collects a biological sample from a human body while attached to the human body. In the following description, sweat exuded from the human body will be described as an example of a biological sample collected by the device 100.

[0011] Here, a description will be given of a usage scenario of the device 100 of this embodiment. In the following description, a person who attaches the device 100 to a body surface may be referred to as a user of the device 100.

[0012] The device 100 of this embodiment may be attached to the user's body surface while the user is sweating a small amount. Specifically, the device 100 may be attached to the user's body surface while the user is showering, bathing, walking, or the like, or after any of these. Note that activities that cause a small amount of sweat are not limited to these, and may include any activity in daily life.

[0013] Device 100 of this embodiment is formed with inlet section 110 and flow path 120. Inlet section 110 is connected to flow path 120, and causes sweat collected from the user's body surface to flow into flow path 120. Flow path 120 retains the sweat that has flowed in from inlet section 110 within device 100. The sweat that has flowed in from inlet section 110 fills toward terminal end 120e of flow path 120 and is retained within flow path 120.

[0014] The device 100 also has a detection unit 130 for detecting sweat. The detection unit 130 of this embodiment is a colorimetric reagent held on a paper substrate, which will be described later, and is provided between the inlet 110 and the terminal end 120e of the flow path 120. The detection unit 130 detects sweat when the sweat that has flowed in from the inlet 110 reaches the detection unit 130. In other words, when the sweat reaches the detection unit 130, the reagent reacts with the sweat and develops a color. Therefore, the sweat that flows from the inlet 110 into the flow path 120 and passes through the detection unit 130 is colored by the reagent.

[0015] Specifically, in this embodiment, sweat filling the flow path 120 from the detection unit 130 to the terminal end 120e is colored by the reagent held by the detection unit 130. In the following description, the part of the flow path 120 from the detection unit 130 to the terminal end 120e will be referred to as a colored unit 140.

[0016] The reagent included in the detection unit 130 of this embodiment may be one that changes color in response to the pH value of the biological sample, one that changes color in response to the concentration of electrolytes (sodium, potassium, chloride ions, etc.) in the biological sample, one that changes color in response to the detection of an amino acid, or cortisol. Examples of amino acids include valine, leucine, isoleucine, etc.

[0017] In this embodiment, an image of the colored portion 140 is taken in the flow channel 120, and the color of the colored portion 140 is analyzed, thereby making it possible to obtain the concentration of the sweat component detected by the detection portion 130, etc.

[0018] Although the device 100 of this embodiment has the detection unit 130, the present invention is not limited to this. The device 100 of this embodiment does not need to have the detection unit 130, and may be used only for collecting biological samples.

[0019] FIG. 2A is a diagram illustrating the configuration of the device of the first embodiment, and FIG. 2B is a cross-sectional view of the device of the first embodiment taken along line AA.

[0020] The device 100 of this embodiment includes a first adhesive layer 101 , a paper substrate 102 , a second adhesive layer 103 , and a cover film 104 .

[0021] The first adhesive layer 101 is formed of an adhesive for the skin to attach the device 100 to the human body. One surface 101a of the first adhesive layer 101 is the surface that adheres to the human body, and the other surface 101b is adhered to one surface 102a of the paper substrate 102.

[0022] The paper substrate 102 is a substrate made of a porous material. The other surface 102b of the paper substrate 102, opposite to the one surface 102a, is adhered to one surface 104a of the cover film 104 via a second adhesive layer 103.

[0023] The second adhesive layer 103 is formed of an adhesive that bonds the paper substrate 102 and the cover film 104. The second adhesive layer 103 may be formed of the same adhesive as the first adhesive layer 101.

[0024] The cover film 104 may be formed of a waterproof base material. In addition, it is preferable that the second adhesive layer 103 and the cover film 104 have high transparency. In this way, when the device 100 is viewed from above, the change in color of the colored portion 140 can be visually observed.

[0025] In the device 100 of this embodiment, the first adhesive layer 101 and the second adhesive layer 103 have hydrophobic properties (hydrophobic function). A general hydrophobic adhesive can be used for the first adhesive layer 101 and the second adhesive layer 103. Furthermore, the first adhesive layer 101 of this embodiment is formed with a collection section 150 for collecting sweat from the body surface into the inlet section 110 when the device 100 is attached to the human body. The collection section 150 is a portion of the first adhesive layer 101 where no adhesive is present, and is formed at a position that overlaps with the inlet section 110 when the device 100 is viewed from above. The collection section 150 may overlap with the inlet section 110 partially or entirely.

[0026] The paper substrate 102 of this embodiment includes a hydrophobic portion 102A having hydrophobic properties and a non-hydrophobic portion 102B, and the hydrophobic portion 102A is formed so as to surround the non-hydrophobic portion 102B. In other words, when viewed in plan, the paper substrate 102 is configured so that the non-hydrophobic region, which is one side or the other of the non-hydrophobic portion 102B, is surrounded by the hydrophobic region, which is one side or the other of the hydrophobic portion 102A.

[0027] Furthermore, the paper substrate 102 is formed so that the hydrophobic portion 102A and the non-hydrophobic portion 102B do not overlap in the stacking direction of the layers of the device 100.

[0028] The non-hydrophobic portion 102B is formed by the inlet portion 110 and the channel 120. In other words, the inlet portion 110 and the channel 120 that form the non-hydrophobic portion 102B are surrounded by the hydrophobic portion 102A.

[0029] In this embodiment, when a user sweats while wearing device 100 on their body surface, the sweat is collected into device 100 through collection unit 150. At this time, because the adhesive surrounding collection unit 150 in first adhesive layer 101 has a hydrophobic function, the sweat collected from collection unit 150 does not soak into first adhesive layer 101, but fills toward inlet portion 110 of paper substrate 102.

[0030] In the paper substrate 102, the inlet section 110 and the flow path 120 are surrounded by the hydrophobic section 102A, and further, the inlet section 110 and the flow path 120 formed in the paper substrate 102 are sandwiched between the first adhesive layer 101 and the second adhesive layer 103, which have hydrophobic properties.

[0031] Therefore, the sweat that flows from the collection section 150 into the inlet section 110 and fills the flow path 120 does not flow out of the flow path 120, but fills towards the terminal end 120e of the flow path 120. Furthermore, because the paper substrate 102 is a porous material, the air present in the flow path 120 is pushed out of the paper substrate 102 as sweat flows in, and the flow is not inhibited by the air present in the flow path 120, and the sweat fills and is retained up to the terminal end 120e of the flow path 120. Furthermore, in this embodiment, the sweat that has filled the flow path 120 is prevented from flowing out of the flow path 120.

[0032] Therefore, according to the device 100 of this embodiment, even if the amount of sweat produced on the user's body surface is small, the sweat can be filled and retained in the flow path 120, and sweat (biological sample) can be collected efficiently.

[0033] Furthermore, in this embodiment, when device 100 is attached to the human body and collection unit 150 is blocked, no liquid other than sweat flows into device 100, and liquid other than sweat does not affect the color development of detection unit 130. Therefore, the accuracy of analysis using an image of coloring unit 140 can be improved compared to, for example, when a sweat outlet or the like is formed in the flow path.

[0034] In this embodiment, the detection unit 130 is provided at a position that is a predetermined distance L away from the inlet 110. The predetermined distance L may be, for example, the distance from the center of the inlet 110 to the end of the detection unit 130. However, the predetermined distance L is not limited to this and may be set arbitrarily.

[0035] This prevents the reagent held in the detection unit 130 from flowing out through the inflow unit 110 and the collection unit 150 and adhering to the human body, thereby improving safety for the user who wears the device 100. Furthermore, this allows a sufficient amount of sweat to be transported to the detection unit 130 when coloring occurs, thereby improving the accuracy of analysis using an image of the coloring unit 140.

[0036] Furthermore, the first adhesive layer 101, the paper substrate 102, the second adhesive layer 103, and the cover film 104 in the device 100 of this embodiment are flexible. As a result, according to this embodiment, the user is less likely to experience pain or itchiness when attaching the device 100 to the skin.

[0037] In this embodiment, sweat has been described as an example of a biological sample, but the biological sample collected by device 100 may be, for example, saliva, tears, blood, interstitial fluid, or the like.

[0038] In addition, in this embodiment, a colorimetric reagent is used as the method for detecting sweat by the detection unit 130, but the method for detecting sweat may be a method other than the method using a colorimetric reagent. For example, the detection unit 130 may be an electrode, and sweat may be detected electrochemically.

[0039] In the device 100 of this embodiment, a cutout may be provided in the second adhesive layer 103 to further improve the visibility of the colored portion 140.

[0040] FIG. 3A is a diagram illustrating another configuration of the device of the first embodiment, and FIG. 3B is another cross-sectional view of the device of the first embodiment taken along line AA.

[0041] 3A and 3B, a cutout portion 160 is formed in the second adhesive layer 103. The cutout portion 160 in this embodiment may be formed so that the second adhesive layer 103 does not overlap with the colored portion 140 in the flow channel 120.

[0042] In device 100A, by providing cutout portion 160 in this manner, the color of colored portion 140 can be seen only through cover film 104, thereby improving visibility.

[0043] Furthermore, by providing the cutout portion 160 in this manner, the color of the colored portion 140 in the image of the colored portion 140 can be made closer to the actual color, and the accuracy of analysis of the color of the colored portion 140 can be improved.

[0044] (Second embodiment) The second embodiment will be described below with reference to the drawings. The second embodiment differs from the first embodiment in that the adhesive layers sandwiching the paper substrate 102 do not have a hydrophobic function. In the following description of the second embodiment, differences from the first embodiment will be described, and components having the same configuration as those in the first embodiment will be assigned the same reference numerals as those used in the description of the first embodiment, and their description will be omitted.

[0045] FIG. 4A is a diagram illustrating the configuration of the device of the second embodiment, and FIG. 4B is a cross-sectional view of the device of the second embodiment taken along line AA.

[0046] In device 100B of this embodiment, first adhesive layer 111 is formed of a skin adhesive for attaching device 100 to the human body. One surface 111a of first adhesive layer 111 is the surface that adheres to the human body, and the other surface 111b is adhered to one surface 112a of paper substrate 112.

[0047] The paper substrate 112 is a substrate made of a porous material. The other surface 112b of the paper substrate 112, opposite to the one surface 112a, is adhered to one surface 104a of the cover film 104 via a second adhesive layer 113.

[0048] The second adhesive layer 113 is formed of an adhesive that bonds the paper substrate 112 and the cover film 104. The second adhesive layer 113 may be formed of the same adhesive as the first adhesive layer 111.

[0049] The cover film 104 may be formed from a waterproof substrate. It is preferable that the second adhesive layer 113 and the cover film 104 have high transparency.

[0050] In device 100B of this embodiment, first adhesive layer 111 and second adhesive layer 113 do not have hydrophobicity (hydrophobic function). Similar to device 100, first adhesive layer 111 of this embodiment is formed with collection section 150 for collecting sweat on the body surface into inlet section 110A.

[0051] In this embodiment, the paper substrate 112 includes hydrophobic portions 112A1 and 112A2, which have hydrophobic properties, and non-hydrophobic portions 112B1 and 112B2, which do not have hydrophobic properties. In this embodiment, the non-hydrophobic portion 112B1 forms the inlet portion 110A, and the non-hydrophobic portion 112B2 forms the flow path 120A.

[0052] In this embodiment, the hydrophobic portion 112A1 is formed so as to partially overlap the non-hydrophobic portion 112B1 in the stacking direction of the layers of the device 100B. More specifically, of the one surface 110a and the other surface 110b of the non-hydrophobic portion 112B1 that forms the inlet portion 110A, an opening 150 is formed on the one surface 110a side, and the hydrophobic portion 112A1 is formed on the other surface 110b. A portion of the hydrophobic portion 112A1 forms a hydrophobic portion between the non-hydrophobic portion 112B1 and the second adhesive layer 113. Furthermore, in this embodiment, the hydrophobic portion 112A2 is formed so as to partially overlap the non-hydrophobic portion 112B2 in the stacking direction of the layers of the device 100B. More specifically, of one surface 120a and the other surface 120b of the non-hydrophobic portion 112B2 that form the flow path 120A, the one surface 120a is adhered to the second adhesive layer 113, and the hydrophobic portion 112A2 is formed on the other surface 120b side. In other words, a portion of the hydrophobic portion 112A2 forms a hydrophobic portion between the non-hydrophobic portion 112B2 and the first adhesive layer 111. Furthermore, a portion of the hydrophobic portion 112A2 is a hydrophobic portion (hydrophobic area) formed on the surface of one side 112a of the paper base material 112, and a portion of the hydrophobic portion 112A1 is a hydrophobic portion (hydrophobic area) formed on the surface of the other side 112b of the paper base material 112. A general hydrophobic adhesive can be used for the hydrophobic portions 112A1 and 112A2.

[0053] That is, in the paper base material 112, when the device 100B is viewed in plan from the cover film 104 side, one surface 120a (non-hydrophobic region) of the non-hydrophobic section 112B2 that forms the flow path 120A is surrounded by the adhesion surfaces (hydrophobic regions) of the hydrophobic sections 112A1 and 112A2 that are bonded to the second adhesive layer 113. In addition, in the paper base material 112, when the device 100B is viewed in plan from the first adhesive layer 111 side, one surface 110a (non-hydrophobic region) of the non-hydrophobic section 112B1 that forms the inlet section 110A is surrounded by the adhesion surfaces (hydrophobic regions) of the hydrophobic sections 112A1 and 112A2 that are bonded to the first adhesive layer 111.

[0054] In this embodiment, by forming hydrophobic portions 112A1 and 112A2 and non-hydrophobic portions 112B1 and 112B2 in this manner, when device 100B is attached to a human body, sweat held in colored portion 140 of flow channel 120A can be prevented from coming into contact with the human body, thereby improving safety. Furthermore, the sweat held in colored portion 140 of flow channel 120A is held within flow channel 120A by cover film 104 and hydrophobic portions 112A1 and 112A2, and therefore the sweat held in flow channel 120A can be prevented from evaporating or leaking to the outside.

[0055] As described above, according to this embodiment, even if first adhesive layer 111 and second adhesive layer 113 do not have a hydrophobic function, it is possible to prevent the reagent from coming into contact with the human body and to prevent sweat filled in flow channel 120A from leaking out of flow channel 120A. Therefore, according to this embodiment, a biological sample can be collected efficiently.

[0056] In addition, in device 100B of this embodiment, cutouts may be provided in second adhesive layer 113 to further improve the visibility of colored portion 140.

[0057] FIG. 5A is a diagram illustrating another configuration of the device of the second embodiment, and FIG. 5B is another cross-sectional view of the device of the second embodiment taken along line AA.

[0058] 5A and 5B, a cutout portion 160 is formed in the second adhesive layer 113. By providing the cutout portion 160 in this manner, the color of the colored portion 140 in the device 100C can be visually recognized only through the cover film 104, and further, the accuracy of analyzing the color of the colored portion 140 can be improved.

[0059] (Third embodiment) A third embodiment will be described below with reference to the drawings. The third embodiment differs from the first embodiment in that the paper substrate does not have a hydrophobic portion. In the following description of the third embodiment, differences from the first embodiment will be described, and components having the same configuration as those in the first embodiment will be assigned the same reference numerals as those used in the description of the first embodiment, and their description will be omitted.

[0060] FIG. 6A is a diagram illustrating the configuration of the device of the second embodiment, and FIG. 6B is a cross-sectional view of the device of the second embodiment taken along line AA.

[0061] In device 100D of this embodiment, first adhesive layer 101 is formed of an adhesive for skin, which allows device 100 to be attached to the human body.

[0062] The paper substrate 122 of this embodiment is a non-hydrophobic substrate made of a porous material. The first adhesive layer 101 and the second adhesive layer 103 are adhesive layers having hydrophobic properties.

[0063] When device 100D is viewed from above, paper base material 122 has a shape that is smaller in area than first adhesive layer 101 and second adhesive layer 103, and is disposed so that its periphery is surrounded by the adhesive surfaces of first adhesive layer 101 and second adhesive layer 103. Therefore, in device 100D of the present embodiment, first adhesive layer 101 and second adhesive layer 103 partially overlap with paper base material 122 in the stacking direction of each layer.

[0064] One surface 122 a of the paper substrate 122 is adhered to the other surface 101 b of the first adhesive layer 101 , and the other surface 122 b of the paper substrate 122 is adhered to one surface 103 a of the second adhesive layer 103 .

[0065] In addition, the other surface 101b of the first adhesive layer 101 is adhered to one surface 103a of the second adhesive layer 103 in an area where the first adhesive layer 101 and the paper substrate 102 do not overlap. The other surface 103b of the second adhesive layer 103 is adhered to one surface of the cover film 104.

[0066] In this embodiment, by laminating the first adhesive layer 101, the paper substrate 122, the second adhesive layer 103, and the cover film 104 in this manner, the paper substrate 122, which forms the inlet section 110 and the flow path 120, is surrounded by the first adhesive layer 101 and the second adhesive layer 103, which have hydrophobic properties.

[0067] Therefore, device 100D of this embodiment can prevent the reagent from coming into contact with the human body, while suppressing sweat filled in flow channel 120 from leaking out of flow channel 120. Therefore, according to this embodiment, a biological sample can be collected efficiently.

[0068] In addition, in the device 100D of this embodiment, a cutout may be provided in the second adhesive layer 103 to further improve the visibility of the colored portion 140.

[0069] FIG. 7A is a diagram illustrating another configuration of the device of the third embodiment, and FIG. 7B is another cross-sectional view of the device of the third embodiment.

[0070] 7A and 7B, a cutout portion 160 is formed in the second adhesive layer 103. In this embodiment, the cutout portion 160 may be formed such that the paper substrate 122 is located inside the cutout portion 160 when the device 100E is viewed in plan.

[0071] Therefore, in device 100E, the other surface 122b of paper substrate 122 is adhered to one surface 104a of cover film 104. Because cover film 104 is waterproof, sweat filled in flow path 120 in paper substrate 102 is retained in flow path 120 without leaking out.

[0072] In this embodiment, by providing a cutout portion 160 in the second adhesive layer 103, it is possible to reduce the swelling of the second adhesive layer 103 that occurs when the paper substrate 122 is sandwiched between the first adhesive layer 101 and the second adhesive layer 103.

[0073] Furthermore, in device 100E, by providing cutout portion 160 in this manner, the color of colored portion 140 can be seen only through cover film 104, and the accuracy of analyzing the color of colored portion 140 can be further improved.

[0074] FIG. 8A is an example of a BB cross-sectional view of the device according to the third embodiment, and FIG. 8B is another example of a BB cross-sectional view of the device according to the third embodiment.

[0075] 8A, no cutout portion 160 is formed. Therefore, in device 100D, a difference in thickness occurs between the portion where paper substrate 122 overlaps with first adhesive layer 101 and second adhesive layer 103 and the portion where paper substrate 122 does not overlap. More specifically, in device 100D, when paper substrate 122 is sandwiched between first adhesive layer 101 and second adhesive layer 103, a bulge occurs by the thickness of paper substrate 122.

[0076] In contrast, in device 100E shown in Figure 8B, paper substrate 122 is placed inside cutout portion 160 formed in second adhesive layer 103, and the other surface 122b of paper substrate 122 is adhered to one surface 104a of cover film 104, thereby preventing bulging of paper substrate 122 by the thickness thereof.

[0077] (Fourth embodiment) The fourth embodiment will be described below with reference to the drawings. In the fourth embodiment, the devices described in the first to third embodiments are included in an analysis system that analyzes an image of the coloring unit 140.

[0078] 9 is a diagram showing an example of the system configuration of an analysis system 10. The analysis system 10 of this embodiment includes a device 100, a detector 300, and an analysis apparatus 400.

[0079] In the analysis system 10 of this embodiment, one of the substances contained in the biological sample is set as a target substance, and the concentration of the target substance is measured. In the following description, the target substance, the concentration of which is to be measured, among the substances contained in the biological sample, is referred to as the measurement target.

[0080] In this embodiment, the biological sample may be, for example, sweat collected from a human body. In addition, in this embodiment, the measurement object is a sodium ion (Na + ), and the concentration of the object to be measured is sodium ion (Na + The electrolyte was sodium ion (Na + ), as well as chloride ions (Cl - The measurement object may be pH, an amino acid (for example, valine, leucine, isoleucine, etc.), or cortisol.

[0081] The detector 300 of this embodiment extracts color information indicating the color of the colored portion 140 of the device 100. In other words, the detector 300 extracts color information indicating the color of the reagent that has permeated the substrate placed on the device 100.

[0082] The color information in this embodiment may be, for example, RGB values ​​(R value, G value, B value). Specifically, the detector 300 in this embodiment may be, for example, an imaging device or a spectroscope. The detector 300 in this embodiment may be any device as long as it can detect color information indicating the color of the reagent.

[0083] The analyzer 400 of this embodiment acquires color information of the colored portion 140 of the device 100 from the detector 300, calculates the concentration of the measurement object from the color information, and outputs the result of the measurement.

[0084] The analysis device 400 of this embodiment will be described below. Fig. 10 is a diagram showing an example of the hardware configuration of the analysis device.

[0085] The analysis device 400 of this embodiment includes a processor 41, a memory 42, an auxiliary storage device 43, an I / F (Interface) device 44, a communication device 45, and a drive device 46. The hardware components of the analysis device 400 are connected to each other via a bus 47.

[0086] The processor 41 has various arithmetic devices such as a CPU (Central Processing Unit), a GPU (Graphics Processing Unit), etc. The processor 41 reads various programs (for example, a learning program, etc.) into the memory 42 and executes them.

[0087] The memory 42 has a main storage device such as a ROM (Read Only Memory), a RAM (Random Access Memory), etc. The processor 41 and the memory 42 form a so-called computer, and the processor 41 executes various programs read onto the memory 42, causing the computer to realize the functions of the analysis device 400, which will be described later.

[0088] The auxiliary storage device 43 stores various programs and various data used when the processor 41 executes the various programs.

[0089] I / F device 44 is a connection device that connects analysis device 400 with an operation device 48 and a display device 49, which are examples of external devices. I / F device 44 accepts operations for analysis device 400 via operation device 48. I / F device 44 may also output results of processing by analysis device 400 and display them to an administrator of analysis device 400 via display device 49.

[0090] The communication device 45 is a communication device for communicating with other devices (in this embodiment, the detector 300).

[0091] The drive device 46 is a device for loading a recording medium 50. The recording medium 50 here includes media that record information optically, electrically, or magnetically, such as CD-ROMs, flexible disks, and magneto-optical disks. The recording medium 50 may also include semiconductor memories that record information electrically, such as ROMs and flash memories.

[0092] The various programs to be installed in the auxiliary storage device 43 are installed, for example, by setting the distributed recording medium 50 in the drive device 46 and reading the various programs recorded on the recording medium 50 by the drive device 46. Alternatively, the various programs to be installed in the auxiliary storage device 43 may be installed by being downloaded from a network via the communication device 45.

[0093] Next, the functions of the analysis device 400 of this embodiment will be described with reference to Fig. 11. Fig. 11 is a diagram illustrating the functional configuration of the analysis device.

[0094] The analysis device 400 of this embodiment includes a color information acquisition unit 410 , a concentration calculation unit 420 , and an output unit 430 .

[0095] The color information acquisition unit 410 of this embodiment acquires the color information extracted by the detector 300 from the coloring unit 140 of the device 100 .

[0096] In addition, the color information of this embodiment may be, for example, the RGB values ​​of the image of the coloring section 140 extracted from image data showing an image of the device 100 captured after the reagent contained in the coloring section 140 reacts with the biological sample, if the detector 300 is an imaging device.

[0097] Furthermore, if the detector 300 is an imaging device, the color information may include luminance indicating the brightness of the image of the coloring unit 140, color difference, hue, saturation, brightness, and gray value.

[0098] The concentration calculation section 420 calculates the concentration of the object to be measured using, for example, the color information acquired by the color information acquisition section 410.

[0099] Specifically, the concentration calculation section 420 of this embodiment analyzes the color information acquired by the color information acquisition section 410, and acquires the concentration of the object to be measured as the measurement result.

[0100] The concentration calculation unit 420 of this embodiment may be realized by, for example, a trained model generated by machine learning using pre-created training data. In this case, the concentration calculation unit 420 may input the color information acquired by the color information acquisition unit 410 to the trained model, and acquire the concentration of the object to be measured output from the trained model as the measurement result.

[0101] Furthermore, when the relationship between the color and concentration of the reagent is expressed by a simple regression equation, the concentration calculation unit 420 can use this regression equation and does not need to use a trained model.

[0102] The output section 430 outputs the concentration of the measurement object calculated by the concentration calculation section 420.

[0103] Next, the processing of the analysis device 400 of this embodiment will be described with reference to Fig. 12. Fig. 12 is a flowchart illustrating the processing of the analysis device.

[0104] The analysis device 400 of this embodiment acquires color information of the colored portion 140 of the device 100 extracted by the detector 300 using the color information acquisition unit 410 (step S1201). The analysis device 400 and the detector 300 may be connected by, for example, wireless communication, and the color information may be acquired by the analysis device 400 via communication.

[0105] Next, the analysis device 400 calculates the concentration of the measurement object using the color information acquired in step S1201 by the concentration calculation unit 420 (step S1202).

[0106] Next, the analyzer 400 outputs the concentration of the measurement object acquired by the concentration calculation section 420 via the output section 430 (step S1203).

[0107] The process of Fig. 12 will be specifically described below. In the following description, the detector 300 will be described as an imaging device.

[0108] In device 100, when sweat reaches detection unit 130, the reagent contained in detection unit 130 develops color, and the sweat is colored and fills colored unit 140. Detector 300 captures image data of device 100 after color of colored unit 140 has changed.

[0109] The color information acquisition unit 410 of the analysis device 400 acquires image data from the detector 300, and identifies an image of the coloring unit 140 from among the images represented by the acquired image data. Specifically, for example, the color information acquisition unit 410 may identify a rectangular image included in the image of the device 100, the long side of which is sufficiently longer than the short side, as the image of the coloring unit 140.

[0110] Next, the color information acquisition unit 410 extracts color information from the identified image. Specifically, the color information acquisition unit 410 may extract RGB values, brightness, color difference, hue, saturation, lightness, gray value, etc. of the identified image as color information.

[0111] When the color information acquisition unit 410 acquires the color information of the coloring unit 140, the analysis device 400 causes the concentration calculation unit 420 to analyze the color information and acquire the pH value, sodium ion concentration, and the like as the results.

[0112] In the above description, image data is acquired from the detector 300, and the color information of the colored portion 140 is extracted in the analysis device 400, but this is not limiting. The extraction of the color information may be performed in the detector 300.

[0113] Furthermore, in the above description, color information of the coloring unit 140 is extracted based on image data of the device 100, and the extracted color information is input into the trained model, but this is not limited to this.

[0114] In this embodiment, image data captured by the detector 300 may be input directly to the trained model. In this case, the trained model may extract color information from the input image data.

[0115] In this way, in this embodiment, when measuring the concentration of the target substance contained in a biological sample, the concentration of the target substance can be obtained using the color information of the reagent after it has reacted with the target substance contained in the biological sample.

[0116] (Fifth embodiment) The fifth embodiment will be described below with reference to the drawings. In the fifth embodiment, a colorimetric sensor 200 is used in place of the device 100 in the analysis system 10.

[0117] The colorimetric sensor of this embodiment will be described below with reference to Fig. 13 to Fig. 15. Fig. 13 is a first diagram illustrating the structure of the colorimetric sensor, Fig. 14 is a second diagram illustrating the structure of the colorimetric sensor, and Fig. 15 is a third diagram illustrating the structure of the colorimetric sensor.

[0118] The colorimetric sensor 200 detects substances and properties contained in the biological sample based on the color change of each colorimetric reagent. In the following description, sweat will be used as an example of the biological sample.

[0119] The colorimetric sensor 200 of this embodiment has a detection unit 230. The detection unit 230 is formed by impregnating a reagent into a substrate and sealing it therein.

[0120] The number of detection units included in the colorimetric sensor 200 is not limited to one as shown in FIG. 1, and may be any number.

[0121] Furthermore, the colorimetric sensor 200 may include a detection unit (reagent) that changes color depending on, for example, lactic acid, uric acid, protein, lipid, ketone, hormone, mRNA, iron, etc. FIG. 14 is an AA cross-sectional view of the colorimetric sensor 200, and FIG. 15 is a schematic exploded view of the AA cross-section.

[0122] The colorimetric sensor 200 of this embodiment includes a cover film 206, an adhesive layer 205, a first layer substrate 204, an adhesive layer 203, a second layer substrate 202, and an adhesive layer 201. The colorimetric sensor 200 of this embodiment also includes a sweat flow path 207.

[0123] The adhesive layer 201 is formed of a skin adhesive for attaching the colorimetric sensor 200 to the human body. One surface 201a of the adhesive layer 201 is the surface that adheres to the human body, and the other surface 201b is adhered to one surface 202a of the second-layer substrate 202.

[0124] Second-layer base material 202 is formed of, for example, filter paper, nonwoven fabric, etc. Second-layer base material 202 has surface 202b opposite surface 202a, which is bonded to one surface 203a of adhesive layer 203.

[0125] The adhesive layer 203 is formed of an adhesive that bonds the second layer substrate 202 and the first layer substrate 204, and the other surface 203b is adhered to one surface 204a of the first layer substrate 204.

[0126] First layer substrate 204 includes detection unit 230 that is impregnated with a reagent and sealed therein, and the other surface 204b is bonded to one surface 205a of adhesive layer 205. In first layer substrate 204, detection unit 230 is a non-hydrophobic portion having non-hydrophobic properties, and region 204A surrounding detection unit 230 is a hydrophobic portion having hydrophobic properties. In this embodiment, the first layer substrate 204 may be formed of the same material as the second layer substrate 202. By forming the second layer substrate 202 and the first layer substrate 204 from the same material, the cost and man-hours required for manufacturing the colorimetric sensor 200 can be reduced.

[0127] The adhesive layer 205 is formed of an adhesive that bonds the first layer substrate 204 and the cover film 206 together, and the other surface 205 b is bonded to one surface 206 a of the cover film 206 .

[0128] 15, the second layer substrate 202 is formed with a first end 207a, a second end 207b, and a sweat channel 207. In the second layer substrate 202, the first end 207a, the second end 207b, and the sweat channel 207 are non-hydrophobic portions having non-hydrophobic properties, and the region 202A surrounding the first end 207a, the second end 207b, and the sweat channel 207 is a hydrophobic portion having hydrophobic properties. In other words, the sweat channel 207 of this embodiment is formed by surrounding the non-hydrophobic region of the second layer substrate 202 with a hydrophobic region. In addition, the adhesive layer 201 has a first opening 208 formed therein that is connected to the first end 207a of the sweat flow path 207, and the adhesive layer 203 has a second opening 209 formed therein that is connected to the second end 207b of the sweat flow path 207.

[0129] That is, the first end 207a of the sweat channel 207 is an inlet portion through which sweat flows into the sweat channel 207, and the second end 207b is an outlet portion through which the sweat that has flowed into the sweat channel 207 flows out to the second opening 209. In this embodiment, the shape of the first end 207a may be the same as the first opening 208. The shape of the second end 207b may be different from the shape of the second opening 209.

[0130] In other words, the shape of second end 207b may be different from the shape of contact surface 230a of detection unit 230. Specifically, second end 207b (outlet portion) may be shaped such that the area of ​​second end 207b is smaller than the area of ​​contact surface 230a of detection unit 230. Details of the shape of second end 207b (outlet portion) will be described later.

[0131] When the colorimetric sensor 200 of this embodiment is attached to the human body, sweat exuded from the human body is collected through the first opening 208. In other words, the first opening 208 is a sweat collection unit that collects sweat exuded from the human body.

[0132] The sweat collected from the first opening 208 is filled from the first end 207a of the sweat flow path 207 toward the second end 207b, and when the sweat reaches the second end 207b, the sweat is filled from the second end 207b toward the second opening 209.

[0133] When the sweat filled in second opening 209 comes into contact with detection unit 230, the color of the reagent that has permeated detection unit 230 changes in response to a substance contained in the sweat. In other words, second opening 209 is a sweat contact unit that brings sweat into contact with detection unit 230. Furthermore, contact surface 230a of detection unit 230 is a contact surface that comes into contact with the sweat filled through second opening 209.

[0134] As described above, in the colorimetric sensor 200 of this embodiment, the detection unit 230 is formed in the first layer substrate 204, and the sweat channel 207 is formed in the second layer substrate 202, which is separate from the first layer substrate 204. Both ends of the sweat channel 207 are connected to the first opening 208, which is the sweat collection unit, and the second opening 209, which is the sweat contact unit. In other words, in this embodiment, the substrate is made into two layers, and the detection unit that holds the colorimetric reagent and the thickener is provided in one layer, and the inlet portion (first end 207a) for allowing the biological sample to flow in is formed in the other substrate.

[0135] In this embodiment, the first opening 208 (sweat collection portion) and the second opening 209 (sweat contact portion) are arranged so as not to overlap in the stacking direction of the adhesive layer 201, the second layer base material 202, the adhesive layer 203, and the first layer base material 204.

[0136] In other words, in this embodiment, the first opening 208 (sweat collection section) and the detection section 230 are arranged at positions where they do not overlap in the stacking direction of the layers.

[0137] In this embodiment, by forming the sweat flow path 207 in this manner, the sweat collected from the first opening 208 gradually fills the second opening 209 while spreading from one side 203a of the adhesive layer 203 into the second opening 209, and as the sweat fills the second opening 209, it reaches the contact surface 230a of the detection unit 230.

[0138] Therefore, in this embodiment, when sweat comes into contact with the contact surface 230a of the detection unit 230, the sweat comes into contact with the entire contact surface 230a, and the reagent can develop a substantially uniform color.

[0139] In this embodiment, the detection unit 230 holds a reagent and a thickener. Specifically, in this embodiment, the thickener is held so that its content relative to the total amount (100% by mass) is in the range of 1% to 25%. The thickener in this embodiment may be, for example, polyethylene glycol. The thickener may also be an aqueous thickener such as polyethylene glycol, polyvinyl alcohol, polyvinylpyrrolidone, carboxyvinyl polymer, sodium polyacrylate, methyl cellulose, ethyl cellulose, hydroxyethyl cellulose, hydroxyethyl cellulose, soluble starch, alginate, gelatin, casein, collagen, xanthan gum, dextran, hyaluronic acid, guar gum, locust bean gum, quince seed, carrageenan, or hydroxyethyl methacrylate.

[0140] In this embodiment, by retaining the reagent and thickener in the detection unit 230 in this way, it is possible to prevent the reagent retained in the detection unit 230 from being washed away due to the inflow of sweat into the detection unit 230. Furthermore, in this embodiment, the sweat that reaches the contact surface 230a spreads gently within the detection unit 230 due to the viscosity of the thickener, so that the reagent can develop a substantially uniform color and it is possible to prevent uneven distribution of the reagent due to solution flow.

[0141] In this way, the colorimetric sensor 200 of this embodiment can suppress variations in color development in the detection unit 230. In other words, the colorimetric sensor 200 of this embodiment can suppress color unevenness in the detection unit 230. Therefore, this embodiment can detect with high accuracy a change in color of a reagent that has reacted with a substance contained in a biological sample.

[0142] Next, the shape of the second end 207b (outlet portion) of this embodiment will be described with reference to Figures 16 to 20. In the following description of Figures 4 to 8, the shape of the second opening 209 is assumed to be the same as that of the second end 207b.

[0143] Fig. 16 is a first diagram illustrating the shape of the second end portion, in which Fig. 16(A) shows an example of the shape of the second end portion, and Fig. 16(B) is a diagram illustrating the flow of sweat.

[0144] 16(A) is formed as a ring-shaped flow channel. The outer diameter of the second end 207b1 is preferably equal to or smaller than the diameter of the contact surface 230a of the detection unit 230 when the contact surface 230a is circular.

[0145] Furthermore, at the second end 207b1, the width W1 of the ring-shaped flow channel may be narrower than the width W2 of the sweat flow channel 207. By making the width W2 greater than the width W1, the flow rate of sweat in the y direction in the sweat flow channel 207 is faster than at the circular second end 207b shown in Fig. 15. Therefore, the second end 207b1 is filled with sweat in a shorter time than at the circular second end 207b shown in Fig. 15, and the sweat flows upward.

[0146] Also, in the example of Figure 16, when sweat fills the second end 207b1, the filled sweat flows out to the inside and outside of the flow path formed by the second end 207b1 on the contact surface 230a, as shown by arrows Y1 and Y2 in Figure 16(A).

[0147] At this time, since the outer diameter of the second end 207b1 is equal to or smaller than the diameter of the contact surface 230a of the detection unit 230 when it is circular, the sweat filled in the second end 207b1 flows out in multiple directions and toward the upper layer, as shown by the arrows Y3 in Figure 16(B).

[0148] Therefore, according to this embodiment, sweat that has flowed from the first end 207a (inlet) to the second end 207b1 quickly flows out to the upper layer, and can be absorbed into the entire detection unit 230 before color unevenness occurs in the detection unit 230. Furthermore, according to this embodiment, sweat flows out from multiple directions onto the contact surface 230a in the upper layer, so that the reagent on the contact surface 230a can be prevented from being washed away by the sweat flowing onto the contact surface 230a.

[0149] Therefore, according to this embodiment, the occurrence of color unevenness in the detection unit 230 can be suppressed.

[0150] Furthermore, the shape of second end 207b in this embodiment is not limited to the ring shape shown in Fig. 16. In this embodiment, second end 207b may have any shape as long as it allows sweat to flow in multiple directions relative to upper contact surface 230a. Examples of the shape of second end 207b are shown below.

[0151] 17A and 17B are diagrams (part 1) showing examples of the shape of the second end portion. The second end portion 207b2 shown in Fig. 17A has a fan-like shape, and the area of ​​the second end portion 207b2 is smaller than the area of ​​the contact surface 230a of the detection unit 230. In this way, sweat stored in the second end portion 207b2 flows out in multiple directions relative to the contact surface 230a, as shown by arrows Y4.

[0152] 17(B) has a rod-like shape that is an extension of the sweat flow path 207. In this case, the sweat filled in the second end 207b3 flows out in multiple directions relative to the contact surface 230a, as shown by arrows Y5.

[0153] 17(C) is formed as a spiral-shaped flow path, and the area of ​​the flow path forming the second end 207b4 is set smaller than the area of ​​the contact surface 230a of the detection unit 230. Furthermore, it is preferable that the width of the flow path forming the second end 207b4 is narrower than the width of the sweat flow path 207. In this way, sweat quickly fills the entire second end 207b4.

[0154] Moreover, the sweat that has filled the second end 207b4 flows out in multiple directions relative to the contact surface 230a, as indicated by the arrows Y6.

[0155] 18A and 18B are diagrams showing examples of the shape of the second end portion (part 2). In the examples shown in Fig. 18A and 18B, the second end portion 207b is formed as a polygonal flow channel.

[0156] 18(A) is formed as a rectangular flow path, and the area of ​​the flow path forming the second end 207b5 is set smaller than the area of ​​the contact surface 230a of the detection unit 230. In addition, it is preferable that the width of the flow path forming the second end 207b5 is narrower than the width of the sweat flow path 207. In this way, sweat quickly fills the entire second end 207b6.

[0157] The sweat that has filled the second end 207b5 flows out to the inside and outside of the flow path formed by the second end 207b5, as indicated by arrows Y7 and Y8, and heads toward the upper layer.

[0158] 18(B) is formed as a hexagonal flow path. In this way, when the second end 207b is formed as a polygonal flow path, the polygon may be any polygonal shape.

[0159] 19A to 19E are diagrams showing examples of the shape of the second end portion 207b. In the examples shown in Fig. 19A to Fig. 19E, the second end portion 207b is formed as a flow path that branches the sweat flowing in from the sweat flow path 207 at the entrance.

[0160] In the second end 207b7 of FIG. 19(A), when sweat flows from the sweat flow channel 207 to the second end 207b7, the flow of sweat is branched into two channels.

[0161] 19(B), the second end 207b8 branches the flow of sweat into five paths when the sweat flows from the sweat flow path 207 to the second end 207b8. In addition, at the second end 207b8, the paths that branch the sweat flowing from the sweat flow path 207 into five paths are connected to each other at the end of each path.

[0162] 19(C), the second end 207b9 branches the sweat flow into four paths when the sweat flows from the sweat flow path 207 to the second end 207b9. In addition, at the second end 207b9, the paths that branch the sweat flowing from the sweat flow path 207 into four paths are not connected to each other at the end of each path.

[0163] 19(D), when sweat flows from the sweat flow channel 207 to the second end 207b10, the flow of sweat is branched into four channels. Also, at the second end 207b10, each of the four channels is formed in a lattice shape, with each channel connected at multiple points.

[0164] 19(E), the second end 207b11 branches the sweat flow into seven paths when the sweat flows from the sweat flow channel 207 to the second end 207b11. In addition, at the second end 207b11, the paths that branch the sweat flow into seven paths are formed radially from the connecting portion between the sweat flow channel 207 and the second end 207b11.

[0165] It is preferable that the area of ​​each second end 207b shown in Figures 19(A) to 19(E) is smaller than the area of ​​the contact surface 230a, and the width of the flow path of each second end 207b is narrower than the width of the sweat flow path 207.

[0166] Fig. 20 is a diagram (part 4) showing an example of the shape of the second end portion. In the second end portion 207b12 shown in Fig. 20, the flow path forming the second end portion 207b12 is meandering. In the second end portion 207b12 of Fig. 20, it is also preferable that the area of ​​the second end portion 207b12 is smaller than the area of ​​the contact surface 230a, and the width of the flow path of the second end portion 207b12 is narrower than the width of the sweat flow path 207.

[0167] In this manner, in this embodiment, by making the area of ​​second end 207b smaller than the area of ​​contact surface 230a of detection unit 230, sweat can be made to flow in multiple directions from second end 207b to upper contact surface 230a. Furthermore, in this embodiment, when a flow path is formed in second end 207b, the width of the flow path is made narrower than the width of sweat flow path 207, thereby shortening the time it takes for sweat to fill second end 207b.

[0168] Therefore, sweat can be permeated into the entire detection unit 230 before color unevenness occurs, and the occurrence of color unevenness can be suppressed.

[0169] The present invention is not limited to the configurations described in the above embodiments, but may be combined with other elements, etc. These aspects can be changed without departing from the spirit of the present invention, and can be appropriately determined depending on the application form. [Explanation of symbols]

[0170] 10 Analysis System 100, 100A~100D devices 101 1st adhesive layer 102 Paper base material 103 Second adhesive layer 104 Cover Film 110 Inlet 120 flow path 130 Detector 140 Coloring section 150 Collection Department

Claims

1. A device for collecting a biological sample from a living body, comprising: A first adhesive layer, a paper substrate, and a second adhesive layer are laminated from the side of the surface to be attached to the living body, the paper substrate has a hydrophobic portion and a non-hydrophobic portion, the hydrophobic portion surrounding the non-hydrophobic portion; A device, wherein the first adhesive layer has a collection portion for collecting the biological sample at a position that overlaps with a portion of the non-hydrophobic portion of the paper base material in a planar view.

2. A device for collecting a biological sample from a living body, comprising: A first adhesive layer, a paper substrate, and a second adhesive layer are laminated from the side of the surface to be attached to the living body, the paper base material has a shape that is smaller in area in a plan view than the first adhesive layer and the second adhesive layer, the first adhesive layer has a collection portion for collecting the biological sample at a position overlapping a part of the paper base material in a plan view, The device, wherein the first adhesive layer and the second adhesive layer are hydrophobic.

3. The paper base material has an inlet portion formed at a position overlapping a part of the collection portion in the stacking direction of the first adhesive layer, the paper base material, and the second adhesive layer; a flow path connected to the inflow portion, through which the biological sample collected from the collection portion flows in via the inflow portion; The device according to claim 1 or 2, wherein a detection unit for detecting the biological sample is disposed on the flow channel.

4. The device according to claim 3 , wherein the detection portion is disposed at a predetermined distance from the inlet portion.

5. a waterproof cover film is adhered to a surface of the second adhesive layer opposite to the surface adhered to the paper base material; The device of claim 3, wherein a cutout portion is formed in the flow path from the position where the detection unit is arranged to the end of the flow path so that it does not overlap with the second adhesive layer in the stacking direction.

6. The paper base material has an inlet portion formed at a position overlapping a part of the collection portion in the stacking direction of the first adhesive layer, the paper base material, and the second adhesive layer; a flow path connected to the inflow portion, through which the biological sample collected from the collection portion flows in via the inflow portion; the non-hydrophobic portion is formed by the inlet portion and the flow path, The device of claim 1 , wherein the first adhesive layer and the second adhesive layer are hydrophobic.

7. a waterproof cover film is adhered to the second adhesive layer on a surface opposite to the adhesive surface to be adhered to the paper substrate; The paper base material has an inlet portion formed at a position overlapping a part of the collection portion in the stacking direction of the first adhesive layer, the paper base material, and the second adhesive layer; a flow path connected to the inflow portion, through which the biological sample collected from the collection portion flows in via the inflow portion; the non-hydrophobic portion is formed by the inlet portion and the flow path, The device of claim 1, further comprising a hydrophobic portion formed on the surface of the inlet portion opposite the adhesive surface with the first adhesive layer and on the surface of the flow path opposite the adhesive surface with the second adhesive layer.

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