Oral composition

The oral composition with collagen, barley grass, dietary fiber, oligosaccharides, and lactic acid bacteria, optionally with ceramide and acerola, addresses the inadequacies of existing formulations by providing enhanced beauty and flavor benefits.

JP2026065703APending Publication Date: 2026-04-15TOYO SHINYAKU KK
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Patent Information

Authority / Receiving Office
JP · JP
Patent Type
Applications
Current Assignee / Owner
TOYO SHINYAKU KK
Filing Date
2026-01-21
Publication Date
2026-04-15

AI Technical Summary

Technical Problem

Existing oral compositions containing collagen and barley grass do not provide sufficient beauty and flavor improvement effects.

Method used

An oral composition comprising collagen, young barley leaves, water-soluble dietary fiber, oligosaccharides, and lactic acid bacteria, optionally with ceramide and acerola, optimized with specific component ratios and amounts to enhance cosmetic effects and taste.

Benefits of technology

The composition exhibits excellent cosmetic effects by enhancing cell activation, promoting collagen absorption, and improving the taste, odor, and texture of collagen-containing beverages.

✦ Generated by Eureka AI based on patent content.

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Abstract

To provide an oral composition that has cosmetic effects and improved taste. [Solution] An oral composition comprising collagen, barley grass, water-soluble dietary fiber, oligosaccharides, and lactic acid bacteria. More preferably, an oral composition further comprising one or more selected from ceramide or acerola.
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Description

Technical Field

[0001] The present invention relates to an oral composition containing collagen, young barley leaves, water-soluble dietary fiber, oligosaccharides, and lactic acid bacteria.

Background Art

[0002] Conventionally, a drinking composition for green juice containing collagen and green leaves of plants such as the stems and leaves of barley is known to have excellent beauty and health effects (see, for example, Patent Document 1). It has also been known that combining young barley leaf powder, pork collagen, and fish collagen in a specific ratio improves the odor and taste of collagen (see, for example, Patent Document 2).

Prior Art Documents

Patent Documents

[0003]

Patent Document 1

Patent Document 2

Summary of the Invention

Problems to be Solved by the Invention

[0004] However, those effects are not sufficient, and further improvement is required.

[0005] The problem of the present invention is to provide an oral composition containing collagen, young barley leaves, etc., which has excellent beauty effects and flavor improvement effects. <0000​​​​​​​​​In other words, the present invention is as follows: [1] An oral composition containing collagen, barley grass, water-soluble dietary fiber, oligosaccharides, and lactic acid bacteria. [2] The oral composition according to [1], further comprising one or more selected from ceramide or acerola. [3] The oral composition according to [1] above, comprising one or more collagens selected from those derived from pigs or fish. [4] The oral composition according to [1] above, characterized in that the amount of barley grass is 10% by mass or more. [5] The oral composition according to [1] above, characterized in that the water-soluble dietary fiber is indigestible dextrin. [6] The oral composition according to [1] above, characterized in that the oligosaccharide is at least one oligosaccharide selected from those with three or more sugars. [7] The oral composition according to [1] above, characterized in that the lactic acid bacteria is at least one lactic acid bacterium selected from the genera Bifidobacterium and Bacillus. [Effects of the Invention]

[0008] According to the present invention, an oral composition with excellent cosmetic effects can be provided by containing collagen and barley grass, etc. Furthermore, according to the present invention, an oral composition can be provided in which the taste, such as the characteristic odor, throat feel, and aftertaste of collagen, has been improved. [Modes for carrying out the invention]

[0009] The oral composition of the present invention is characterized by containing collagen, young barley grass, water-soluble dietary fiber, oligosaccharides, and lactic acid bacteria.

[0010] The oral composition of the present invention exhibits excellent cosmetic effects. Specifically, the oral composition of the present invention enhances the cell activation activity of human dermal fibroblasts and promotes collagen absorption, thereby exhibiting excellent cosmetic effects.

[0011] Therefore, the oral composition of the present invention can be used as a cosmetic composition, a collagen absorption composition, and the like.

[0012] Furthermore, by combining collagen with barley grass and other materials, the oral composition of the present invention improves the taste of collagen-containing oral compositions, providing an oral composition that is easy to drink.

[0013] The following describes each component contained in the oral composition of the present invention (hereinafter sometimes referred to as "components of the present invention"). [collagen] The collagen may be animal-derived or synthetic, and may include collagen protein, collagen peptides obtained by hydrolyzing collagen protein, and atelocollagen obtained by treating collagen molecules with protease and removing the telopeptide portion. Among animal-derived collagen, collagen derived from pigs and fish is particularly preferred. The average molecular weight (weight-average molecular weight) of the collagen is not particularly limited, but is preferably 500 to 100,000, and more preferably 1,000 to 50,000.

[0014] [Barley grass] Barley (Hordeum vulgare L.) is an annual or biennial herbaceous plant belonging to the grass family, native to Central Asia, and is broadly classified into two-row barley and six-row barley depending on the shape of the ear. The barley grass used in the oral composition of the present invention is not particularly limited as long as it is commonly available, and barley grass of any variety, such as two-row barley or six-row barley, may be used. Stems may also be included along with the leaves.

[0015] In the present invention, barley grass can be, for example, pulverized, juiced, or extracted. Examples of pulverized barley grass include dried powder, flaked barley grass and its dried form (dried flaked barley grass), and granules. Juice and extracts may be in liquid form, but can also be used as a paste or dried powder (juice powder, extract powder). Extracts can be obtained by extraction using a suitable solvent, and examples of solvents that can be used include water (warm water, hot water), ethanol, and aqueous ethanol. In the present invention, it is preferable to use dried barley grass powder (barley grass pulverized powder) in order to better enjoy the effects of the present invention.

[0016] [Water-soluble dietary fiber] Water-soluble dietary fiber refers to water-soluble components (dietary fiber) in food that are not digested by human digestive enzymes. The water-soluble dietary fiber used in this invention is not particularly limited and includes, for example, indigestible dextrin, pectin, guar gum, guar gum hydrolysate (guar bean enzyme hydrolysate), agarose, glucomannan, polydextrose, alginic acid and its salts, β-glucan, inulin, carrageenan, fucoidan, and derivatives thereof. These can be used individually or in combination of two or more, and it is preferable to use them in combination of two or more. In this invention, it is preferable to use indigestible dextrin in order to better enjoy the effects of the present invention.

[0017] [Oligosaccharides] In this invention, oligosaccharides refer to short sugars, which are compounds formed by glycosidic bonds between three to twenty sugars. Specifically, examples of oligosaccharides in this invention include, but are not limited to, indigestible oligosaccharides such as lactulose oligosaccharide, fructooligosaccharide, galactooligosaccharide, xylooligosaccharide, and soybean oligosaccharide, and digestible oligosaccharides such as maltooligosaccharide, isomaltoligosaccharide, beet oligosaccharide (raffinose), lactulose, and lactitol. The number of constituent sugars in the oligosaccharide is preferably three to eighteen, more preferably three to fourteen, even more preferably three to ten, and particularly preferably three to seven.

[0018] In the present invention, from the viewpoint of being able to more enjoy the effects of the present invention, it is preferable to use isomaltooligosaccharide or beet oligosaccharide (raffinose). Further, it is preferable to combine two or more kinds of oligosaccharides, and it is more preferable to combine two or more kinds of digestible oligosaccharides. As the physiological functions of indigestible oligosaccharides, there are effects such as not being decomposed by human digestive enzymes, reaching the large intestine, contributing to the activation and growth of intestinal bifidobacteria, and improving the intestinal environment. As the physiological functions of digestible oligosaccharides, there is an effect of being decomposed by human digestive enzymes, absorbed in the stomach and small intestine, and serving as an energy source.

[0019] [Lactic acid bacteria] In this invention, "lactic acid bacteria" refers to a general term for bacteria that produce lactic acid through metabolism, and is a concept that includes Bifidobacteria. Examples of lactic acid bacteria include those belonging to the genera Bifidobacterium, Lactbacillus, Enterococcus, Leuconostoc, Pediococcus, Staphylococcus, Tetragenococcus, Leuconostoc, Pediococcus, Staphylococcus, Tetragenococcus, and Bacillus. Bifidobacterium genus includes Bifidobacterium bifidum, Bifidobacterium breve, Bifidobacterium infantis, Bifidobacterium lactis, Bifidobacterium longum, Bifidobacterium adolescentis, Bifidobacterium mongoliense, Lactbacillus genus includes Lactbacillus brevis, Lactbacillus gasseri, Lactobacillus acidophilus, Lactobacillus buchneri, Lactobacillus bulgaricus, Lactobacillus delburvecki, Lactobacillus casei, Lactobacillus crispatus, Lactobacillus curvatus, Lactobacillus halivaticus, Lactobacillus pentosus, Lactobacillus plantarum, Lactobacillus paracasei, Lactobacillus rhamnosus, Lactobacillus salivarius, Lactobacillus sporogenes, Lactobacillus sakei, Lactobacillus fructivorans, Lactobacillus hilgardii, Lactobacillus reuteri, Lactobacillus fermentum, Enterococcus faecalis(Sometimes referred to as Streptococcus faecalis), Enterococcus faesium (Sometimes referred to as Streptococcus faesium), among the Streptococcus genus, Streptococcus thermophilus, Lactococcus lactis (Sometimes referred to as Streptococcus lactis), among the Leuconostoc genus, Leuconostoc mesenteroides, Leuconostoc oenos, among the Pediococcus genus, Pediococcus acidilactici, Pediococcus pentosaceus, among the Staphylococcus genus, Staphylococcus carnosus, Staphylococcus xylosus, among the Tetragenococcus genus, Tetragenococcus halophilus, among the Bacillus genus, Bacillus coagulans, Bacillus mesentericus, etc. can be mentioned. These lactic acid bacteria can be used alone or in combination of two or more, and it is preferable to use them in combination of two or more. The method for obtaining lactic acid bacteria is not particularly limited, and for example, lactic acid bacteria isolated from foods such as yogurt and vegetables or commercially available products can be used.

[0020] In the present invention, from the point that the effects of the present invention can be more enjoyed, lactic acid bacteria of the Bifidobacterium genus and the Bacillus genus are preferable, and Bifidobacterium longum and Bacillus coagulans (spore-forming lactic acid bacteria) are particularly preferable.

[0021] [Ceramide] In the oral composition of the present invention, it is preferable to include ceramide in addition to the above-mentioned components of the present invention. In the present invention, in order to better enjoy the effects of the present invention, in addition to human-type ceramides such as ceramide 1, ceramide 2, and ceramide 3, animal-derived ceramides extracted from the brains and spinal cords of cattle, horses, pigs, etc., and plant-derived ceramides extracted from wheat, rice, soybeans, spinach, corn, konjac, pineapple, etc. may be used as ceramides. Furthermore, the ceramide used in the present invention may also be a glycoceramide, and specifically, for example, those to which monosaccharides such as galactosylceramide and glucosylceramide are bonded, or those to which oligosaccharides are bonded, can be cited.

[0022] In the composition of the present invention, it is preferable to use plant-derived ceramides, and more preferably to use glucosylceramides derived from grasses such as wheat, corn, and rice. Among these, it is particularly preferable to use glucosylceramide extracted from rice, which is obtained from rice, as this allows for a higher efficacy of the present invention. Examples of solvents used for extraction include water; lower alcohols such as ethanol, methanol, isopropanol, and butanol; lower esters such as ethyl acetate and methyl acetate; acetone; and mixed solvents of these with water. Ethanol or aqueous ethanol is preferred because it allows for efficient extraction of the active ingredient. The temperature of the extraction solvent can be appropriately set between room temperature and below the boiling point, depending on the solvent used.

[0023] [Acerola] In the oral composition of the present invention, it is preferable to include acerola in addition to the above-mentioned components of the present invention. Acerola is a fruit tree belonging to the Malpighiaceae family that inhabits tropical regions, and its scientific name is Malpighia emarginata. The fruit of acerola is rich in vitamin C and polyphenols. In the present invention, the fruit is preferred as the part of the acerola used in order to better enjoy the effects of the present invention. Furthermore, in the present invention, various processed products of acerola can be used, and the preferred processed forms are crushed or crushed powder, juice or juice powder, extract or extract powder, and it is more preferable to use juice or juice powder. In the present invention, it is most preferable to use fruit juice or juice powder in order to better enjoy the effects of the present invention.

[0024] In the oral composition of the present invention, the amount of collagen is preferably 25% by mass or more, more preferably 35% by mass or more, and even more preferably 45-65% by mass, in order to better enjoy the effects of the present invention.

[0025] Furthermore, the amount of barley grass in the oral composition of the present invention is preferably 5% by mass or more, more preferably 10% by mass or more, and even more preferably 15 to 50% by mass, in order to better enjoy the effects of the present invention.

[0026] Furthermore, the optimal mass ratio of the three components of the present invention other than collagen and barley grass is, for example, 1:20 to 0.0001 for water-soluble dietary fiber and oligosaccharides, preferably 1:15 to 0.001, and more preferably 1:10 to 0.01. For example, 1:15 to 0.0005 for water-soluble dietary fiber and lactic acid bacteria, preferably 1:10 to 0.005, and more preferably 1:5 to 0.05. Furthermore, for example, 1:50 to 0.001 for lactic acid bacteria and oligosaccharides, preferably 1:25 to 0.01, and more preferably 1:5 to 0.1.

[0027] Furthermore, when the oral composition of the present invention contains ceramide, the amount blended is preferably 0.00001% by mass or more, more preferably 0.0001% by mass or more, and even more preferably 0.001 to 1% by mass, in order to better enjoy the effects of the present invention.

[0028] Furthermore, when the oral composition of the present invention contains acerola, the amount blended is preferably 0.0001% by mass or more, more preferably 0.001% by mass or more, and even more preferably 0.01 to 1% by mass, in order to better enjoy the effects of the present invention.

[0029] There are no particular restrictions on the amount of the oral composition of the present invention to be taken, but in order to better enjoy the effects of the present invention, it is preferable to take an amount of 2 g / day or more per day, more preferably 3 g / day or more, and even more preferably 4 g / day or more. The oral composition of the present invention can be contained in one container, or divided into, for example, two or three containers, to constitute one day's dose, so that the daily intake amount is as described above.

[0030] As described above, the oral composition of the present invention can be used as a cosmetic composition, a collagen absorption composition, a collagen absorption promoting composition, etc., and can be used as a so-called health food, such as a functional food whose efficacy has been approved by a designated institution, such as a Food for Specified Health Uses, a Food with Nutrient Function Claims, or a Food with Function Claims, or as a pharmaceutical product (including quasi-drugs).

[0031] The cosmetic composition of the present invention is not particularly limited as long as it contains the ingredients of the present invention and is distinguishable from other products as a product in that it is used for cosmetic purposes. For example, any product in which the body, packaging, instructions, or promotional materials (advertising media) of the present invention displays that it has cosmetic effects is included within the scope of the present invention. Furthermore, in the case of functional foods, this includes those in which cosmetic effects are the scientific basis for the functionality.

[0032] Furthermore, the cosmetic compositions of the present invention are not limited to those in which the ingredients of the present invention are indicated as active ingredients on the product packaging, etc. For example, they may not specify an active ingredient. Also, even general food products are included in the scope of the present invention if they are manufactured and sold with indications of their use. For example, food products that are sold with testimonials on websites, etc., mentioning beauty effects as personal impressions of people who have consumed them are also included in the scope of the present invention.

[0033] Examples of the cosmetic compositions of the present invention include so-called health foods that display phrases such as "For those who want to live beautifully and healthily," "Supporting youthful days," "To maintain youthfulness," "For those concerned about moisture," "For those concerned about beauty and health," "For those who want to take in nutrients and beauty ingredients together," and "For those who want to remain beautiful even as they age."

[0034] Examples of the oral composition of the present invention include tablets, capsules, powders, granules, liquids, granules, rods, plates, blocks, solids, rounds, pastes, creams, caplets, gels, jellies, chewables, and sticks. Among these, tablets, capsules, powders, granules, liquids, and jellies are preferred because they allow for greater enjoyment of the effects of the present invention. Specifically, examples include supplements, beverages in containers such as PET bottles, cans, and glass bottles, instant beverages (powdered beverages) to be dissolved in water (hot water), milk, fruit juice, green juice, etc., and jelly-like foods and beverages. These are preferred because they are easy to consume with meals and enhance palatability.

[0035] The oral composition of the present invention can be manufactured by adding other components as needed and by known methods such as mixing.

[0036] The oral composition of the present invention is particularly preferably used as a granule. The granulated composition has improved dispersibility in liquids and ease of drinking, making it suitable for use as a powdered beverage, and can be used as a beverage granule. A beverage granule refers to a granule (a powdered beverage that is a granule) that consumers mix with a liquid such as water, hot water, milk, or soy milk when ingested and consume as a beverage.

[0037] The granulation method is not particularly limited and can be appropriately selected from granulation methods commonly used by those skilled in the art. Examples include fluidized bed granulation, extrusion granulation, rolling granulation, and agitation granulation. Regardless of the granulation method, granulated material can be produced by using any granulation apparatus available on the market and appropriately setting various manufacturing conditions such as drying temperature. From the viewpoint of obtaining granulated material with excellent ease of granulation and dispersibility, fluidized bed granulation is particularly preferred. Fluidized bed granulation is a method in which powder particles to be used as raw materials are fluidized by blowing air onto them and spraying a binding liquid such as water, thereby binding the powder particles together and forming granulated material, which is an aggregate of powder particles. [Examples]

[0038] The present invention will be described in more detail below based on examples, but the present invention is not limited thereto. [Test 1] The cell-activating activity of normal human dermal fibroblasts (NHDF) was confirmed using the composition of the present invention. Normal human dermal fibroblasts are widely used as an excellent model system for studying cell physiology, and by confirming their cell-activating activity, it is possible to confirm cosmetic effects such as improved skin hydration through the maintenance and improvement of skin function.

[0039] The test substances used in this experiment are as follows: For the collagen, we used commercially available fish-derived collagen and pig-derived collagen. Specifically, we used fish-derived collagen peptide, which was obtained by washing fish scales and skin with water, extracting with hot water, breaking it down into low molecular weight molecules with enzymes, purifying it, and then powdering it. We also used pig-derived collagen peptide, which was obtained by washing pigskin with water, acid-treating it, extracting gelatin with hot water, breaking it down into low molecular weight molecules with enzymes, purifying it, and then powdering it.

[0040] For the barley grass, dried and pulverized young barley grass powder (manufactured by Toyo Shinyaku Co., Ltd.) was used. Specifically, fresh young barley grass was cut, blanched, dried, and then pulverized, and the dried and pulverized powder that passed through a sieve with a mesh size of 1 mm or less was used.

[0041] As a water-soluble dietary fiber, commercially available indigestible dextrin made from tapioca starch, with a dietary fiber content of 38-45% as measured by enzyme-liquid chromatography, was used.

[0042] Commercially available beet oligosaccharides (raffinose) and isomaltoligosaccharides were used as oligosaccharides.

[0043] Commercially available Bifidobacterium longum and Bacillus coagulans (spore-forming lactic acid bacteria) were used as lactic acid bacteria. Specifically, Bifidobacterium longum was used as a dried powder of live bacteria with a bacterial count of 150 billion cells / g or more, and Bacillus coagulans (spore-forming lactic acid bacteria) was used as dried bacterial cells with a lactobacillus spore count of 6 billion cells / g or more.

[0044] As the ceramide, commercially available rice-derived ceramide was used. Specifically, an extract containing 10% or more glucosylceramide obtained by extracting from the seeds of rice Oryza sativa Linne (Gramineae) with ethanol was used. For the acerola, we used commercially available acerola concentrate powder. Commercially available powdered dextrin was used as the dextrin.

[0045] The specific experimental procedure is as follows: (1) 75cm in a 37℃, 5% CO2 incubator 2 Commercially available normal human dermal fibroblasts were cultured in flasks. The culture medium used was 10% FBS-DMEM.

[0046] (2) Cells suspended by trypsin treatment, 75 cm 2 Transfer 1.5 × 10⁶ of liquid from the flask to a 96-well plate. 3 Cells were seeded at a cell density of cells / well and pre-cultured for 24 hours in a 37°C, 5% CO2 incubator.

[0047] (3) After removing the culture medium, the test substances were mixed in the ratios shown in Table 1 below, and 100 μL / well of the culture medium containing the test substances was added. The mixture was then incubated at 37°C in a 5% CO2 incubator for 24 hours.

[0048] (4) After 24 hours of incubation, the culture medium was removed and the mixture was washed with PBS. (5) 100 μL / well of a 500 μg / mL MTT (Nacalai Tesque) solution prepared with serum-free DMEM was added to each well, and the cells were allowed to stand in a 37°C, 5% CO2 incubator to stain them appropriately. (6) After culturing, the MTT solution was removed and the mixture was washed with PBS.

[0049] (7) 100 μL / well of isopropanol was added, and it was confirmed that the dye had eluted extracellularly. The absorbance at 570 nm and 650 nm was then measured.

[0050] (8) Based on the obtained data, the percentage of control (relative number of viable cells with the number of viable cells in the control set to 100%) was calculated, and the cell activation activity was evaluated for each example and comparative example. The results are shown in Table 1 as relative values ​​with the value of Comparative Example 1 set to 100.

[0051] % of control = (Data sample - Data blank) / (Data control - Data blank) × 100

[0052] [Table 1]

[0053] As shown in Table 1, the compositions of the examples containing the five components of the present invention showed synergistically improved cell activation activity compared to the compositions of the comparative examples that did not contain some of the components of the present invention. Specifically, comparing Comparative Example 1, which contained two components and did not contain water-soluble dietary fiber, lactic acid bacteria, and oligosaccharides, with Examples 1 and 2, which contained five components including collagen and barley grass in addition to these, Examples 1 and 2 showed improved cell activation activity. Similarly, comparing the results of Examples 1 and 2 and Example 3, the addition of two types of collagen further improved cell activation activity. Similarly, comparing the results of Examples 3 and 4, the addition of two types of lactic acid bacteria and two types of oligosaccharides further improved cell activation activity. Furthermore, comparing the results of Examples 3 and 5 and 6, the addition of ceramide or acerola further improved cell activation activity. Finally, comparing the results of Examples 4-6 and Example 7, the addition of ceramide and acerola particularly improved cell activation activity. The composition of the present invention has been shown to enhance the cell-activating activity of normal human dermal fibroblasts, which are an excellent model system for studying cell physiology, and has been confirmed to exhibit cosmetic effects such as improved skin hydration through the maintenance and improvement of skin function.

[0054] [Exam 2] The present invention's composition was used to confirm the collagen absorption-promoting effect of monolayer permeability in human colorectal cancer-derived cell lines. Monolayer permeability in human colorectal cancer-derived cell lines is a common in vitro model used to evaluate the absorption of compounds through the human gut. By confirming collagen permeability, it is possible to confirm cosmetic effects such as improved skin firmness and elasticity due to enhanced collagen absorption.

[0055] The test substance used in this experiment was the same as that used in Test 1.

[0056] The specific experimental procedure is as follows: (1) 75cm in a 37℃, 5% CO2 incubator 2 Commercially available human colorectal cancer-derived cell lines were cultured in flasks. The culture medium used was 10% FBS-1% NEAA-DMEM.

[0057] (2) Place 4 × 10 cells, suspended by trypsin treatment, into insert wells on a 24-well plate. 4 Cells were seeded at a cell density of cells / well and pre-cultured for 24 hours in a 37°C, 5% CO2 incubator. (3) After 24 hours, the culture medium was changed, and thereafter the culture was continued for 21 days, changing the medium every 3-4 days, until a monolayer was formed. (4) After the monolayer was formed, the electrical resistance was measured and the material was washed with HBSS buffer.

[0058] (5) The test substances were mixed in the ratios shown in Table 2 below, and 50 μL / well of culture medium containing the test substances was added. The mixture was incubated at 37°C in a 5% CO2 incubator for 2 hours. HBSS buffer was used as the culture medium, and the total concentration of the test substances in the medium was set to 1000 μg / mL.

[0059] (6) The amount of collagen in the permeate was measured using a commercially available kit, and the collagen absorption amount is shown in Table 2 as a relative value with the value of Comparative Example 2 set to 100.

[0060] [Table 2]

[0061] As shown in Table 2, the compositions of the examples containing the five components of the present invention synergistically improved collagen absorption compared to the compositions of the comparative examples that did not contain some of the components of the present invention. Specifically, comparing Comparative Example 2, which contained two components and did not contain water-soluble dietary fiber, lactic acid bacteria, and oligosaccharides, with Examples 8 and 9, which contained five components including collagen and barley grass in addition to these, collagen absorption improved in Examples 8 and 9. Similarly, comparing the results of Examples 8 and 9 and Example 10, the addition of two types of collagen further improved collagen absorption. Also similarly, comparing the results of Examples 10 and 11, the addition of two types of lactic acid bacteria and two types of oligosaccharides further improved collagen absorption. Furthermore, comparing the results of Examples 10 and Examples 12 and 13, the addition of ceramide or acerola further improved collagen absorption. Finally, comparing the results of Examples 11-13 and Example 14, the addition of ceramide and acerola particularly improved collagen absorption. The composition of the present invention improved the monolayer permeability of collagen in human colon cancer-derived cell lines, a common in vitro model used to evaluate the absorption of compounds via the human gut. As a result, it was confirmed that the composition exhibits cosmetic effects such as improved skin firmness and elasticity through enhanced collagen absorption.

[0062] [Exam 3] The taste-improving effect was confirmed using the composition of the present invention. The test substance used in this experiment was the same as that used in Test 1.

[0063] Each sample was prepared by mixing 5g of the powder sample with 150ml of water according to the mixing ratios listed in Table 3. Of these samples, the sample obtained from the powder sample of Comparative Example 2 was used as the standard. Sensory evaluation was conducted by panelists who regularly consume green juice and have experience in sensory evaluation. The panelists were asked to evaluate the characteristic odor of collagen, throat feel, texture, and aftertaste.

[0064] <Collagen-specific odor> ◎: It has no unpleasant odor and tastes considerably better than the standard product. ○: It has less of an unpleasant odor and tastes better than the standard product. △: Comparable to the standard product. ×: Below standard quality.

[0065] <Smooth throat feel> ◎: It has a smooth texture and feels much easier to drink than the standard product. ○: It has a smooth texture and feels easier to drink than the standard product. △: Comparable to the standard product. ×: Below standard quality.

[0066] <Texture> ◎: It has a pleasant texture and feels considerably easier to drink than the standard product. ○: It has a pleasant texture and feels easier to drink than the standard product. △: Comparable to the standard product. ×: Below standard quality.

[0067] <Aftertaste> ◎: It has a pleasant aftertaste and leaves the mouth feeling much cleaner than the standard product. ○: It has a pleasant texture and a cleaner taste compared to the standard product. △: Comparable to the standard product. ×: Below standard quality.

[0068] "comprehensive evaluation" The overall evaluation was determined based on the following criteria, considering the characteristic odor of collagen, throat feel, texture, and aftertaste. The results are shown in Table 3 above. ◎: Excellent, with no × or △ marks. ○: There are no × or △ marks, indicating a good result. △: There are △'s but no ×'s, so it is acceptable. ×: There is one or more × marks, so it is not acceptable.

[0069] [Table 3]

[0070] As shown in Table 3, the compositions of the examples containing the five components of the present invention showed improved taste compared to the comparative examples that did not contain some of the components of the present invention. Specifically, when comparing Comparative Example 3, which contained two types of collagen and did not contain other components, with Comparative Example 4, which also contained barley grass, the collagen odor was reduced in Comparative Example 4, but other evaluation items such as throat feel were about the same as in Comparative Example 3. On the other hand, Example 15, which contained the five components of the present invention, was superior in all evaluation items when compared with the results of Comparative Examples 3 and 4. In other words, while the taste improvement effect was insufficient with just two components, collagen and barley grass (Comparative Examples 3 and 4), the addition of water-soluble dietary fiber, lactic acid bacteria, and oligosaccharides to make a total of five components improved the taste in all evaluation items. Furthermore, when comparing the results of Examples 15 and 16, it was found that by using lactic acid bacteria and two types of oligosaccharides, and adding ceramide and acerola, the taste was further improved, resulting in a superior, easy-to-drink composition.

[0071] [Manufacturing Examples 1-3] (Manufacturing of powdered beverages) As shown in Table 4, collagen and other raw materials were mixed and granulated to produce a powdered beverage. When 5g of the resulting powdered beverage was mixed with 150mL of water and consumed, it was found to have excellent effects in maintaining and / or improving skin moisture and firmness, and was easy to drink.

[0072] [Table 4] [Industrial applicability]

[0073] The oral composition of the present invention has high industrial utility because it can be used as a health food or the like.

Claims

1. An oral composition containing 25% or more by mass of collagen, barley grass, water-soluble dietary fiber, at least one oligosaccharide selected from fructooligosaccharides, isomaltoligosaccharides, and beet oligosaccharides, and lactic acid bacteria.

2. The oral composition according to claim 1 further comprises one or more selected from ceramide or acerola.

Citation Information

Patent Citations

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