Cell culture device

Abstract

This invention provides a cell culture device that can accurately evaluate the effects of air quality on cultured cells. [Solution] The cell culture apparatus 1 comprises a sprayer 11 that sprays a suspension containing fine particles to generate a mist, a diffusion dryer 12 that dries the mist to generate an aerosol, a cylindrical chamber 20 for culturing cells to evaluate the test air containing the aerosol, and a suction pump 13 for introducing the test air into the chamber 20. The chamber 20 has an annular mounting section 221 on which a plurality of culture containers 31 for culturing cells are placed, an air supply section 211 that introduces the test air from a plurality of air inlets 211a arranged at approximately equal intervals in the circumferential direction at the top of the chamber 20 and exposes it to the culture containers 31, and an exhaust section 222 that discharges the test air from the bottom of the chamber 20. The exhaust section 222 also includes a plurality of first exhaust sections 222a arranged at approximately equal intervals in the circumferential direction at the bottom of the mounting section 221.

Claims

1. A sprayer that generates mist by spraying a suspension containing fine particles, A diffusion dryer that dries the mist to generate an aerosol, A cylindrical chamber for culturing cells to evaluate the test air containing the aerosol, The system includes a suction pump for introducing the air to be tested into the chamber, The aforementioned chamber is The chamber includes an annular mounting section on which multiple culture vessels for culturing cells are placed, An air supply unit is provided at the top of the chamber, which introduces the air to be tested from a plurality of air supply ports arranged at approximately equal intervals in the circumferential direction and exposes it to the culture vessel. The chamber has an exhaust section at the bottom for drawing in the air to be tested using the suction pump and discharging it, The exhaust section includes a first exhaust section in which a plurality of exhaust ports are arranged at approximately equal intervals in the circumferential direction at the lower part of the mounting section described above. A cell culture apparatus characterized by the following features.

2. The aforementioned exhaust section is The central part of the mounting section includes a second exhaust section, the exhaust port of which is located below the upper surface of the mounting section described above. The cell culture apparatus according to feature 1.

3. A light source is positioned outside the chamber and irradiates the culture vessel with light, The system includes an anti-fog unit positioned on the irradiation surface of the chamber, which is irradiated with light from the light source, and which suppresses fogging by heating the irradiation surface. A cell culture apparatus according to claim 1 or 2.

4. The anti-fogging unit includes a Peltier element that heats the irradiation surface. The cell culture apparatus according to feature 3.

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