Deamidation methods for proteins

Abstract

This invention provides a method for promoting deamidation reactions by protein deamidation enzymes. [Solution] The present invention aims to accelerate the deamidation reaction by acting a protein deamidation enzyme on a protein in the presence of salts or polysaccharides. According to the present invention, an improvement in reaction efficiency can be expected. The present invention is particularly useful for the deamidation of plant proteins such as pea protein. Deamidated proteins (especially deamidated plant proteins) obtained by applying the present invention can be used in various foods or beverages.

Claims

1. A method for deamidating proteins, in which protein glutaminase acts on a protein in the presence of polysaccharides.

2. The protein deamide method according to claim 1, wherein protein glutaminase is applied to a protein in the presence of polysaccharides and salts.

3. The protein deamide method according to claim 2, wherein the salt is at least one selected from the group consisting of carbonates, phosphates, hydrogen phosphates, polyphosphates, and citrates.

4. The protein deamide method according to any one of claims 1 to 3, wherein the protein is a plant protein and / or an animal protein.

5. A protein modifier containing polysaccharides and protein glutaminase.

6. Furthermore, the protein modifier according to claim 5, further containing salts.

7. The protein modifier according to claim 6, wherein the salts are carbonates, phosphates, hydrogen phosphates, polyphosphates, or citrates.

8. A method for producing a deamidated protein, comprising the following steps: (1) A step of preparing a solution containing protein and polysaccharides, (2) The process of treating the prepared solution with protein glutaminase.

9. A method for manufacturing food or pharmaceuticals, including the following steps: (1) A step of preparing a food ingredient or pharmaceutical ingredient containing protein, and a solution containing polysaccharides, (2) The process of treating the prepared solution with protein glutaminase.

10. The solution prepared in step (1) The manufacturing method according to claim 8 or 9, further comprising salts.

11. The manufacturing method according to claim 10, wherein the salt is at least one selected from the group consisting of carbonates, phosphates, hydrogen phosphates, polyphosphates, and citrates.

12. The method for producing a protein according to any one of claims 8 to 11, wherein the protein is a plant protein and / or an animal protein.

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