Methods for treating skin conditions or diseases

Abstract

The present invention provides compositions and methods for orally and / or topically treating skin diseases and conditions, such as acne, atopic dermatitis, psoriasis, facial redness, folliculitis, dry skin, itchy skin, hyperpigmentation, aging, wrinkles, etc., by administering to a subject in need thereof a pharmaceutical composition or dietary supplement comprising an in vitro grown grape cell line culture. Additionally, the present invention provides methods and compositions for treating skin conditions and diseases, such as acne, atopic dermatitis, psoriasis, facial redness, folliculitis, dry skin, itchy skin, aging, wrinkles, etc., by topically administering to a subject in need thereof a pharmaceutical composition or cosmetic composition comprising an in vitro grown grape cell line culture.

Description

[Background technology]

[0001] There are a variety of skin conditions that cause discomfort or embarrassment to individuals, either cosmetically or as a result of disease. Among these conditions are acne, rosacea, aging, skin discoloration and photodamage, psoriasis, dandruff and eczema, facial redness, dermatitis, etc.

[0002] Acne vulgaris is an inflammatory skin disorder that frequently occurs during adolescence. The condition can include skin lesions ranging from comedones in hair follicles to more severe conditions such as pustules, papules, cysts, and nodules. The condition can be uncomfortable and embarrassing, and can lead to scarring. The initial pathology is the formation of comedones (more commonly called whiteheads) and blackheads, which are composed of hard keratinous masses that block hair follicles and are associated with increased sebum production.

[0003] Another skin condition requiring treatment is rosacea, a common chronic skin condition characterized by a range of clinical signs including flushing attacks, erythema, telangiectasias, an acne-like inflammatory papulopustular rash, and ocular manifestations.

[0004] Psoriasis is a chronic, widespread skin disorder that affects millions of humans and even livestock. The disorder is characterized by recurrent, raised, red lesions, plaques, or, rarely, pustules, on the skin. The plaques are the result of excessively rapid proliferation and shedding of epithelial (skin) cells.

[0005] Eczema (including but not limited to atopic, nummular, and hand eczema) often has similar overlapping characteristics as psoriasis. They may occur together, or the disease may begin as eczema and transform over time into psoriasis. Treatment is corticosteroids and similar formulations commonly used for both of these conditions.

[0006] The most common form of seborrheic dermatitis (seborrhea) is simple dandruff. This condition can become more severe and cause scaly, red patches to form on the face, ears, chest, and other widespread areas. It often occurs in conjunction with psoriasis.

[0007] In addition to the above conditions, skin aging is a complex phenomenon that can result in changes to the skin.

[0008] Although there are many treatments described in the art for improving the appearance and condition of skin, there remains a need for compositions with improved effectiveness without undesirable side effects. Summary of the Invention

[0009] In some embodiments, methods are provided for treating a skin disease, condition, or disorder in a subject in need thereof, the methods comprising administering to the subject a composition comprising an effective amount of an in vitro grown red grape cell culture.In some embodiments, methods are provided for treating a skin disease, condition, or disorder in a subject in need thereof, the methods comprising administering to the subject an effective amount of a composition comprising an in vitro grown red grape cell culture.

[0010] In some embodiments, methods are provided for treating a skin disease, condition, or disorder in a subject in need thereof, the method comprising administering to the subject a composition comprising an effective amount of an in vitro grown red grape cell culture and a pharmaceutically or dietary acceptable carrier.

[0011] In some embodiments, the skin disease, condition, or disorder is one or more of acne, atopic dermatitis, psoriasis, facial redness, folliculitis, dry skin, itching skin, and hyperpigmentation. In some embodiments, a method of treating a skin condition or disorder is provided, comprising topically administering to a subject a composition comprising an effective amount of an in vitro grown red grape cell culture and a cosmetically or pharmaceutically acceptable carrier.

[0012] In some embodiments, the composition is in a form for oral administration.

[0013] In some embodiments, the in vitro grown red grape cell culture is grown in a bioreactor.

[0014] In some embodiments, the in vitro grown red grape cell culture is in the form of a powder comprising a cell line culture of grape cells grown in vitro in a large scale up process, the cell line culture of grape cells being derived from one or more of a grape-berry cross section, a grape-berry skin, a grape-berry flesh, a grape seed, a grape embryo of seeded or seedless cultivars, or a grape seed coat, and the cell line culture of grape cells comprises resveratrol in an amount of at least 1000 mg / kg powder. In some embodiments, the composition is or comprises Vinia®. In some embodiments, the composition is in a form for topical administration.

[0015] In some embodiments, the composition is or comprises Vinia®.

[0016] In some embodiments, a cosmetic composition is provided comprising an effective amount of in vitro grown red grape cells. In some embodiments, the composition is an oral composition in the form of a capsule comprising an in vitro grown red grape cell culture.

[0017] In some embodiments, the capsule contains 100-800, 100-700, 100-600, 100-500, 200-400, 200-700, 200-600, 200-500, or 200-400 mg of dried powder of in vitro grown red grape cells.

[0018] In some embodiments, the capsule contains about 100, 200, 200, 400, 500, or 600 mg of dried powder of in vitro grown red grape cells.

[0019] In some embodiments, the capsule contains about 100, 200, 200, 400, 500, or 600 mg of dried powder of in vitro grown red grape cells containing 20-70, 20-60, 20-50, or 20-40 mg of polyphenols.

[0020] In some embodiments, the capsule contains about 100, 200, 200, 400, 500, or 600 mg of dried powder of in vitro grown red grape cells containing 10-60, 10-50, or 10-40 mg of polyphenols.

[0021] In some embodiments, the capsule contains about 100, 200, 200, 400, 500, or 600 mg of dried powder of in vitro grown red grape cells containing 2-12, 3-10, 4-8, or 5-7 mg of polyphenols.

[0022] In some embodiments, the capsule contains about 100, 200, 200, 400, 500, or 600 mg of dried powder of in vitro grown red grape cells containing about 2, 3, 4, 5, 6, 7, 8, or 10 mg of piseidresveratrol.

[0023] In some embodiments, the capsule contains about 100, 200, 200, 400, 500, or 600 mg of dried powder of in vitro grown red grape cells containing about 6 mg of piseidresveratrol.

[0024] In some embodiments, the capsule contains about 400 mg of dried powder of red grape cells containing about 6 mg of piseidresveratrol and 20-40 mg of polyphenols.

[0025] In some embodiments, the in vitro grown red grape cell culture is grown in a bioreactor.

[0026] In some embodiments, the in vitro grown red grape cell culture is in the form of a powder comprising a cell line culture of grape cells grown in vitro in a large-scale scale-up process, the cell line culture of grape cells being derived from one or more of grape berry cross sections, grape berry skins, grape berry pulp, grape seeds, grape embryos of seeded or seedless cultivars, or grape seed skins, and the cell line culture of grape cells comprises resveratrol in an amount of at least 1000 mg / kg powder. In some embodiments, the composition is or comprises Vinia®. In some embodiments, a topical composition for treating skin or scalp is provided, the topical composition comprising an effective amount of in vitro grown red grape cell culture and a cosmetically or pharmaceutically acceptable carrier.

[0027] In some embodiments, the in vitro grown red grape cell culture is grown in a bioreactor.

[0028] In some embodiments, the in vitro grown red grape cell culture is in the form of a powder comprising a cell line culture of grape cells grown in vitro in a large-scale scale-up process, wherein the cell line culture of grape cells is derived from one or more of grape berry cross sections, grape berry skins, grape berry pulp, grape seeds, grape embryos of seeded or seedless cultivars, or grape seed skins, and the cell line culture of grape cells comprises resveratrol in an amount of at least 1000 mg / kg powder. In some embodiments, the composition is or comprises Vinia®. In some embodiments, the topical or cosmetic composition is in the form of a cream, facial cleanser or shampoo, soap, cleanser, antiperspirant, deodorant, sunscreen, astringent, lotion, moisturizer, serum, mask, face or body cosmetic, ointment, lotion, gel, foam, solution, or powder. In some embodiments, a composition is provided for treating a skin disease, condition, or disorder in a subject in need thereof, comprising an in vitro grown red grape cell culture and a pharmaceutically or food acceptable carrier.

[0029] In some embodiments, a composition is provided comprising an in vitro grown red grape cell culture for treating a skin disease, condition, or disorder in a subject in need thereof.

[0030] In some embodiments, the skin disease, condition, or disorder is one or more of acne, atopic dermatitis, psoriasis, facial redness, folliculitis, dry skin, or itchy skin.

[0031] In some embodiments, the composition is in a form suitable for oral administration.

[0032] In some embodiments, the in vitro grown red grape cell culture is grown in a bioreactor. In some embodiments, the in vitro grown red grape cell culture is in the form of a powder comprising a cell line culture of grape cells grown in vitro in a large-scale scale-up process, the cell line culture of grape cells being derived from one or more of grape berry cross sections, grape berry skins, grape berry pulp, grape seeds, grape embryos of seeded or seedless cultivars, or grape seed skins, and the cell line culture of grape cells comprises resveratrol in an amount of at least 1000 mg / kg powder. In some embodiments, the composition is or comprises Vinia®.

[0033] In some embodiments, a cosmetic composition is provided comprising an in vitro grown red grape cell culture and a cosmetically acceptable carrier for treating, reducing, alleviating, mitigating, or eliminating, or for eliminating skin aging, wrinkles, facial redness, hyperpigmentation, and skin blemishes in a subject in need thereof.

[0034] In some embodiments, the oral composition is in the form of a capsule comprised of in vitro grown red grape cell cultures.

[0035] In some embodiments, the in vitro grown red grape cell culture is grown in a bioreactor.

[0036] In some embodiments, the in vitro grown red grape cell culture is in the form of a powder comprising a cell line culture of grape cells grown in vitro in a large scale-up process, the cell line culture of grape cells being derived from one or more of grape berry cross sections, grape berry skins, grape berry pulp, grape seeds, grape embryos of seeded or seedless cultivars, or grape seed skins, and the cell line culture of grape cells comprises resveratrol in an amount of at least 1000 mg / kg powder. In some embodiments, the composition in the capsule is Vinia®. DETAILED DESCRIPTION OF THE INVENTION

[0037] The present invention provides compositions and methods for orally and / or topically treating skin diseases and conditions, such as acne, atopic dermatitis, psoriasis, facial redness, folliculitis, dry skin, itchy skin, and hyperpigmentation, by administering a pharmaceutical composition or dietary supplement comprising an in vitro-grown grape cell line culture to a subject in need thereof. Additionally, the present invention provides methods and compositions for treating skin conditions or diseases, such as acne, atopic dermatitis, psoriasis, facial redness, folliculitis, dry skin, itchy skin, hyperpigmentation, aging, or wrinkles, by topically administering a pharmaceutical or cosmetic composition comprising an in vitro-grown grape cell line culture to a subject in need thereof. In some embodiments, a subject in need thereof can be treated with both a topical and oral composition comprising an in vitro-grown grape cell line culture.

[0038] definition As used herein, the terms "about" and "approximately" are used interchangeably and, when used to refer to modifying a numerical value, encompass a numerical uncertainty range of 0% to 10% of that value.

[0039] As used herein, the singular forms "a," "an," and "the" include plural referents unless the context clearly dictates otherwise.

[0040] The terms "treat," "treating," and "treatment" in the context of treating a disease, disorder, or condition are meant to include alleviating, alleviating, ameliorating, or eliminating a skin disorder, disease, or condition, or one or more symptoms associated with a skin disorder, disease, or condition, or to slow the progression of the disease, disorder, or condition, or one or more symptoms thereof.

[0041] As used herein, the terms "subject," "individual," or "patient" are used interchangeably and refer to any animal, including mammals such as mice, rats, other rodents, rabbits, dogs, cats, pigs, cows, sheep, horses, primates, and humans. In some embodiments, the patient is a human. In some embodiments, the subject is experiencing and / or exhibiting at least one symptom of the skin disease, condition, or disorder to be treated and / or prevented.

[0042] The term "ameliorate" is used to convey that the methods or compositions of the present invention change any of the characteristics and / or physical attributes of the tissue to which it is provided, applied, or administered. The term "alleviate" can also be used in conjunction with disease states, where, when a disease state is "ameliorated," the symptoms or physical characteristics associated with the disease state are reduced or eliminated.

[0043] The term "inhibit" generally refers to preventing the onset of symptoms, alleviating symptoms, or eliminating a disease, condition, or illness.

[0044] "Pharmaceutically acceptable," "physiologically acceptable," and grammatical variations thereof refer to compositions, carriers, diluents, and reagents or other ingredients of a formulation.

[0045] As used herein, the term "cosmetically acceptable" and grammatical variations thereof refer to compositions, carriers, diluents, and reagents or other ingredients of a formulation.

[0046] As used herein, the term "therapeutic agent" refers to an in vitro grown grape cell line used to treat, overcome, alleviate, prevent, or ameliorate an undesirable skin condition or skin disease in a subject.

[0047] In part, embodiments of the present invention relate to rosacea, including, but not limited to, rosacea-associated papules, pustules, mastoid skin (skin thickening), telangiectasias, or erythema, other skin erythemas, telangiectasias, purpura, and the like, and other symptoms associated therewith; including, but not limited to, keratosis, facial disseminated miliary lupus, eczema, dermatitis (contact dermatitis, atopic dermatitis, seborrheic dermatitis, nummular eczema, generalized exfoliative dermatitis, stasis dermatitis, neurodermatitis, lichen simplex chronicus, dry and xerotic dermatitis), impaired sweating and sweating.

[0048] Other inflammatory skin conditions, including dermatitis, asteatotic eczema, etc., or other conditions characterized by hypersensitive skin or epithelial barrier disorders; skin diseases characterized by hyperkeratotic skin, such as rough, dry, cracked or cracked skin characterized by keratosis, ichthyosis, and ichthyosis-like skin diseases; hair follicle and sebaceous gland diseases, such as acne, perioral dermatitis, and pseudochoriocystitis; sweat gland diseases, such as rashes including crystalline, erythematous, deep, and pustular eruptions; sunburn, chronic actinic radiation injury, poikiloderma, radiation dermatitis, photopurpura, etc. For the treatment of various skin diseases, conditions, or disorders, or symptoms thereof, including psoriasis, drug eruptions, erythema multiforme, erythema nodosum, and granuloma annulare; skin reactions and conditions; vascular extravasation of any size or cause of bleeding due to any skin injury which may include any trauma including blistering, ecchymosis, purpura, etc., or any trauma including surgical or procedural trauma including any blood accumulation in the resulting skin diseases and conditions characterized by bruising; infection, inflammatory skin disease, or inflammation of any cause, or a combination thereof.

[0049] Some embodiments may relate to a cream formulation having moisturizing properties, the cream comprising an in vitro grown red grape cell line.

[0050] As used herein, the terms "therapeutically effective" or "effective" may be used interchangeably and include therapeutic compositions of the present invention. For example, a therapeutically effective amount of a composition is the amount of a composition comprising an in vitro grown grape cell line that generally achieves a desired effect.

[0051] "Topical application" refers to applying or coating a composition on the surface of keratinous tissue. "Topical skin compositions" include compositions suitable for topical application to keratinous tissue. Such compositions are typically dermatologically acceptable in that they do not have undue toxicity, incompatibility, instability, allergic reactions, etc. when applied to the skin.

[0052] Further embodiments of the present invention relate to methods for treating erythema, redness, or telangiectasia associated with rosacea, comprising administering a cream comprising a therapeutically effective amount of oxymetazoline. Some embodiments relate to methods for treating papules, pustules, and other inflammatory lesions associated with rosacea, comprising administering a cream comprising a therapeutically effective amount of in vitro-grown red grape cell culture. Some embodiments relate to methods for treating skin erythema, comprising administering a cream comprising a therapeutically effective amount of in vitro-grown red grape cell culture. Some embodiments relate to methods for treating purpura, comprising administering a cream comprising a therapeutically effective amount of in vitro-grown red grape cell culture. Some embodiments relate to the treatment of ciliary keratosis, lupus miliaris disseminatus faciens, and the like, comprising administering a cream comprising a therapeutically effective amount of in vitro-grown red grape cell culture. Some embodiments are directed to the treatment of redness or erythema, skin erythema, telangiectasia, purpura, etc. associated with rosacea, and other related indications; including, but not limited to, keratosis, lupus miliaris disseminatus faciei, eczema, dermatitis (including contact dermatitis, atopic dermatitis, seborrheic dermatitis, nummular eczema, generalized exfoliative dermatitis, stasis dermatitis, neurodermatitis, lichen simplex chronicus, dry skin, etc.); other conditions characterized by irritation of the skin or epithelial barrier; dermatological diseases and other inflammatory skin conditions including xerosis, dyshidrosis and hidrotic dermatitis, asteatotic eczema; diseases characterized by dry, cracked or chapped skin, keratosis and hyperkeratotic skin diseases, such as ichthyosis and ichthyosiform skin diseases; pseudo-chorionic folliculitis hair follicle and sebaceous gland diseases such as erythema multiforme, erythema nodosum and granuloma annulare, poikiloderma, radiation dermatitis, photopurpura ("solar purpura"), skin reactions and conditions; bleeding or bruising such as petechiae, ecchymoses, purpura, etc., including vascular extravasation of any size or cause, any retention of blood in the skin due to surgery or procedures.Diseases and conditions characterized by bleeding or bruising due to any skin injury, which may include any trauma, relate to methods of treating infections, inflammatory skin diseases or inflammation due to any cause, hi a further embodiment, the formulation is cosmetically acceptable.

[0053] In some embodiments, the methods and compositions of the present invention are suitable for use as a therapeutic agent to reduce discomfort such as, for example, irritation, itching, and redness.

[0054] The compositions of the present invention containing in vitro grown red grape cell cultures for topical administration have a cooling, soothing and cooling effect on the skin and, in pathological conditions, provide anti-irritant, anti-pruritic and / or anti-nociceptive effects.

[0055] In certain embodiments described herein, cream formulations can be used to treat various types of skin conditions.For example, cream formulations can be used to treat rosacea, eczema, dermatitis, atopic dermatitis, psoriasis, steroid-responsive skin diseases, pruritus or dryness.In certain embodiments, cream formulations are applied after physical or mechanical skin trauma, such as shaving (as a "repair agent" after shaving) or tweezers, after bathing, showering, or sweating.In some embodiments, the composition can be used for skin conditions after exposure to external factors, such as sunlight, wind, cold, low humidity, high temperature and humidity, radiation, air pollution, smoke or cigarette smoke.

[0056] In some embodiments, compositions comprising in vitro grown red grape cell cultures may be used in topically applied drug formulations, medications, or topical formulations (such as fragrances, insect repellents, exfoliants, skin scrapes, shaving, or hair removal). In some embodiments, the topical composition containing the in vitro grown red grape cell line is in the form of a cream, facial cleanser or shampoo, soap, cleanser, antiperspirant, deodorant, sunscreen, astringent, lotion, moisturizer, serum, mask, face or body cosmetic, ointment, lotion, gel, foam, solution, or powder. The in vitro grown red grape cell line is typically added to the topical composition in an amount of 1-10%, 2-8%, 2-7%, 2-6%, 2-5%, 2-4%, 1-4%, 1-6%, or 1-8% powder, for example, as described in WO 2014 / 068557. In some embodiments, the in vitro grown red grape cell line powder contains 1000 mg / kg or more of resveratrol.

[0057] In some embodiments, the topical composition is used to treat intrinsic or extrinsic skin aging, including skin thinning, sagging skin, fine lines, fine and deep facial wrinkles, raspberry, or skin tone, or to provide smoothness and softness to the skin. In some embodiments, the pharmaceutical or cosmetic topical composition as defined above, comprising an in vitro grown red grape cell culture, is formulated with an extract of glyceryl stearate and PEG-100 stearate, acetyl alcohol, allantoin, petrolatum, steareth-21, tocopheryl acetate, Lavandula angustifolia (lavandula angustifolia), and / or a mixture of glyceryl stearate and PEG-100 stearate. angustifolia oil, xanthan gum, dipotassium glycyrrhizinate, triethanolamine, bisabolol, disodium EDTA, vitamin B3, anti-irritants, antioxidants, terpenes, cannabis terpenes, anti-skin redness agents, anti-adhesives, binders, coating agents, disintegrants, flavorings, colorants, lubricants, glidants, adsorbents, preservatives, fillers, emulsifiers, moisturizers, thickeners, skin nourishing agents, skin moisturizers, occlusive agents, emollients, soothing agents, natural fragrances, suspending agents, analgesics, pH adjusters, purified water, shea butter, lavender oil, and any combination thereof.

[0058] In some embodiments, topical pharmaceutical or cosmetic compositions comprising the in vitro grown red grape cell cultures defined herein can be used to treat a symptom selected from the group consisting of erythema, redness, induration, thickness, scaling, scaling, red patches of skin covered with silvery scale, small scales, dry skin, cracked skin that may bleed, itching, burning, pain, thickened, pitted or raised nails, and any combination thereof.

[0059] A topical composition comprising an in vitro grown red grape cell culture also provides for the use of the aforementioned composition to treat skin, remove wrinkles, rejuvenate skin, improve skin appearance, prevent skin damage, prevent skin deterioration, or provide nutrients to skin, and any other use described herein.

[0060] Non-limiting examples of skin conditions include dry skin, itchy skin, inflamed skin, erythema, sensitive skin, pruritus, spider veins, lentigines, age spots, senile purpura, keratosis, melasma, pigmentation, fine lines or wrinkles, nodules, sun-damaged skin, dermatitis (including but not limited to seborrheic dermatitis, nummular dermatitis, contact dermatitis, atopic dermatitis, exfoliative dermatitis, perioral dermatitis, and stasis dermatitis), psoriasis, folliculitis, rosacea, acne, impetigo, erythroderma, erythema, eczema, sunburn, inflammatory skin conditions, etc. In certain non-limiting embodiments, the skin condition may be caused by exposure to UV light, age, irradiation, chronic sun exposure, environmental pollutants, air pollution, wind, cold, heat, chemicals, disease pathology, smoking, or nutritional deficiency. The skin can be facial skin or non-facial skin (e.g., arms, legs, hands, chest, back, feet, etc.). The method can further include identifying a person in need of skin treatment. The person can be male or female. The age of the person can be at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, or more, or any range derivable therein.

[0061] Also disclosed is a method for treating hyperpigmentation, comprising applying the composition of the present invention to skin.This method can also include identifying a person who needs hyperpigmentation treatment, and applying the composition to the part of the skin that shows hyperpigmentation.The other method that the inventors contemplate includes applying the composition of the present invention to the skin that needs such treatment, to reduce the appearance of age spots, skin discoloration or freckles, to reduce or prevent the appearance of fine lines or wrinkles on the skin, or to increase skin firmness.

[0062] In one embodiment of the present invention, a method is provided for reducing the appearance of a condition associated with erythema (e.g., erythematous skin, irritated skin, irritated skin) comprising topically applying a composition to erythematous, irritated, or irritated skin, wherein the topical composition comprises an in vitro grown red grape cell culture.

[0063] According to some embodiments, the in vitro grown red grape cell culture is grown in a bioreactor.

[0064] In some embodiments, the in vitro grown red grape cell culture is in the form of a powder comprising a cell line culture of grape cells grown in vitro in a large scale scale-up process, wherein the cell line culture of grape cells is derived from one or more of a grape berry cross section, a grape berry skin, a grape berry pulp, a grape seed, a grape embryo of a seeded or seedless cultivar, or a grape seed skin, and wherein the cell line culture of grape cells comprises resveratrol in an amount of at least 1000 mg / kg powder.

[0065] In some embodiments, the in vitro grown red grape cell culture is Vinia®.

[0066] The composition of the present invention can comprise a carrier.Depending on the type of composition of the present invention, the carrier can be a food-appropriate carrier or a pharmaceutically acceptable carrier, as long as it is compatible with the specific type of composition of the present invention.Examples of food-appropriate carriers include, but are not limited to, food-appropriate excipients, diluents and carriers.Examples of pharmaceutically acceptable carriers include, but are not limited to, biocompatible vehicles, adjuvants, additives and diluents to obtain a composition that can be used as a dosage form.

[0067] The composition containing the in vitro grown grape cell line may be used alone or in combination with other biologically active ingredients. The composition of the present invention, alone or in combination with other active ingredients, may be administered to a subject in a single dose or multiple doses over a period of time, generally orally. Various administration patterns will be apparent to those skilled in the art. The dosage range for administration of the composition of the present invention is sufficient to produce the desired effect. The dosage should not be so large as to cause adverse side effects, such as undesirable cross-reactivity. Generally, the dosage will vary depending on the age, weight, sex, condition, and severity of the condition in the subject, as well as the intended purpose. The dosage can be determined by one of skill in the art without undue experimentation. The dosage may be adjusted in the event of any contraindications, tolerance, or similar conditions. Those skilled in the art can easily evaluate such factors and, based on this information, determine the specific effective concentration of the composition of the present invention to be used for the intended purpose.

[0068] In some embodiments, the in vitro grown fruit cell culture is red grapevine cells (RGCs or RGC products). The RGC powder contains a matrix of polyphenols, including resveratrol, piceidresveratrol, quercetin, anthocyanins, and tannins. In some embodiments, the RGC powder is a powder composition comprising a cell line culture of grapevine cells grown in vitro in a large-scale scale-up process, the cell line culture of grapevine cells being derived from one or more of grapevine berry cross-sections, grapevine berry skins, grapevine berry pulp, grape seeds, grapevine embryos of seeded or seedless cultivars, or grapevine seed skins, and the grapevine cell line culture contains resveratrol in an amount of at least 1000 mg / kg powder. In some embodiments, the RGCs are prepared as described in WO 2014 / 068557. In some embodiments, the RGC powder is equivalent to VINIA®.

[0069] VINIA® is therefore a source of the entire matrix of nutrients and polyphenols found in whole red grapes and red wine in their natural (physicochemical) state without any genetic manipulation, containing polyphenols (resveratrol, piceidresveratrol, tannins, quercetin, catechins, anthocyanins), vitamins, proteins, and fiber. Its advantage is the absence of solvent residues and the typical appearance, color, odor, and flavor.

[0070] The CTFA International Cosmetic Ingredient Dictionary and Handbook (2008), 12th Edition, describes a wide variety of non-limiting cosmetic ingredients that can be used in the context of the topical compositions of the present invention. These ingredient categories include fragrances (artificial and natural), pigments and coloring ingredients (e.g., Blue No. 1, Blue No. 1 Lake, Red No. 40, titanium dioxide, D&C Blue No. 4, D&C Green No. 5, D&C Orange No. 4, D&C Red No. 17, D&C Red No. 33, D&C Purple No. 2, D&C Yellow No. 10, and D&C Yellow No. 11), adsorbents, emulsifiers, stabilizers, lubricants, solvents, humectants (e.g., emollients, humectants, film formers, occlusive agents, and agents that enhance the natural skin tone of the skin). including agents affecting moisturizing mechanisms), water repellents, UV absorbers (physical and chemical absorbers such as para-aminobenzoic acid ("PABA") and corresponding PABA derivatives, titanium dioxide, zinc oxide, etc.), essential oils, vitamins (e.g., A, B, C, D, E, and K), trace metals (e.g., zinc, calcium, and selenium), anti-irritants (e.g., steroidal and non-steroidal anti-inflammatory agents), plant extracts (e.g., aloe vera, chamomile, etc.), , cucumber extract, ginkgo biloba, ginseng, and rosemary), antibacterial agents, antioxidants (e.g., BHT and tocopherol), chelating agents (e.g., disodium EDTA and tetrasodium EDTA), preservatives (e.g., methylparaben and propylparaben), pH adjusters (e.g., sodium hydroxide and citric acid), absorbents (e.g., aluminum starch octenylsuccinate, kaolin, corn starch, oat starch, cyclodextrin, talc, and zeolite), skin bleaching and whitening agents (e.g., hydroquinone and niacinamide lactate), humectants (e.g., glycerin, propylene glycol, butylene glycol, pentylene glycol, sorbitol, urea, and mannitol), exfoliants (e.g., alpha-hydroxy acids and beta-hydroxy acids such as lactic acid, glycolic acid, and salicylic acid;and their salts), waterproofing agents (e.g., magnesium / aluminum hydroxide stearate), skin conditioning agents (e.g., aloe extract, allantoin, bisabolol, ceramides, dimethicone, hyaluronic acid, and dipotassium glycyrrhizinate), thickeners (e.g., substances capable of increasing the viscosity of the composition, such as carboxylic acid polymers, crosslinked polyacrylate polymers, polyacrylamide polymers, polysaccharides, and gums), and silicone-containing compounds (e.g., silicone oils and polyorganosiloxanes);

[0071] Example The following examples further illustrate the embodiments disclosed herein. These examples are provided solely for the purpose of illustrating preferred embodiments of the invention, and are not intended to limit the invention in any manner. [Example]

[0072] Skin studies in Korea with Vinia® capsules: In early 2023, 12 participants from South Korea were recruited from an online community of people with various skin complaints (e.g., acne, atopic dermatitis, psoriasis, facial redness, folliculitis, dry skin, itchy skin, etc.) to evaluate the effectiveness of Vinia® for various skin conditions. Participants had to have a long-term skin-related health complaint (e.g., atopic dermatitis, facial redness, acne, or dry skin). It should be noted that many people with atopic dermatitis have very dry skin due to their skin's inability to retain moisture. After a 10-day selection period, candidates were selected and each was mailed two bottles of Vinia® along with instructions for taking Vinia® in the following manner: for the first 14 days, participants were instructed to take one Vinia® capsule. For the next 21 days, participants were instructed to increase their dosage to two Vinia® capsules per day. All participants were instructed to take Vinia® with water before breakfast. Photographs of the skin areas of greatest concern to participants were taken at various time points during the study (before taking Vinia®, at the end of week 1, week 2, week 3, week 4, and week 5). Two participants could not be contacted, and one participant was unable to report results.

[0073] Nine study participants reported beneficial effects from Vinia® treatment. Five reported high satisfaction. One woman (Subject #9), who had been complaining of facial redness, itching, and allergies, reported a significant improvement in her symptoms, as shown in photographs of her cheeks and chin after Vinia treatment. In her own words, she "felt like she had a 'facial treatment.'" One woman (Subject #4) reported that her facial redness disappeared after taking Vinia for several weeks. She reported that the redness only appeared during exercise and disappeared shortly thereafter, whereas before taking Vinia®, the redness persisted for several days. A 10-year-old woman with severe atopic dermatitis reported a dramatic reduction in her itching and redness. Two males (Subjects #1 and #3) who had been complaining of acne also saw improvement. The remaining subjects reported mild satisfaction. One woman did not experience any improvement in her skin condition, but felt that she had more energy while taking Vinia® (Subject #2 in the table below).

[0074] Three participants reported during the study. A male college student with long-standing atopic dermatitis (Subject #1) reported, "It felt like my skin was covered in something special, something I'd never experienced before." One woman reported feeling as if she had received a "facial treatment." The mother of a 10-year-old female with atopic dermatitis (Subject #6) reported that she could literally "see the skin barrier forming before her eyes."

[0075] Dose Effect: Two of the nine participants (Subjects #1 and #9) reported experiencing better results when they increased their Vinia® dosage from one capsule to two capsules.

[0076] Table 1 below provides an overview of the study. [Table 1]

[0077] The purpose of this study was to evaluate the effects of Vinia® on various skin conditions at specific dosages, durations, and timing of Vinia® intake throughout the day.

[0078] conclusion Results demonstrated that participants taking one capsule of Vinia® for the first 14 days, followed by two capsules per day for the next 21 days, significantly improved skin conditions such as allergies, atopic dermatitis, facial redness, breakouts, acne, itching, and dry skin. Data suggest beneficial effects in both women and men. [Example]

[0079] Skin tests with creams containing Vinia®: A cream containing approximately 2% Vinia® was administered to six subjects over the age of 50 with various skin conditions. All subjects reported improvement in their skin disease or disorder, with one subject (#1) reporting that Vinia® helped with facial redness, dry skin, and blisters, while other topical treatments (steroids, antibiotics) did not help at all.

[0080] The study aimed to collect information on the effects of creams containing Vinia® on skin conditions such as facial redness, dry skin, skin blisters, red spots, hyperpigmentation, and wrinkles, and evaluated the duration of treatment. [Table 2]

[0081] Conclusion: The results demonstrate that a cream containing Vinia® significantly improved skin conditions such as facial redness, dry skin, skin blisters, red spots, hyperpigmentation, and wrinkles in participants. The data suggest beneficial effects in both women and men.

[0082] While the present invention has been described in conjunction with its detailed description, it should be understood that the foregoing description is intended to illustrate, but not limit, the scope of the invention, which is defined by the appended claims. Other aspects, advantages, and modifications are within the scope of the following claims.

Claims

1. 1. A method of treating a skin disease, condition, or disorder in a subject in need thereof, comprising administering to the subject an effective amount of a composition comprising an in vitro grown red grape cell culture.

2. 1. A method of treating a skin disease, condition, or disorder in a subject in need thereof, comprising administering to the subject a composition comprising an effective amount of an in vitro grown red grape cell culture and a pharmaceutically or food acceptable carrier.

3. 3. The method of claim 1 or 2, wherein the skin disease, condition, or disorder is one or more of acne, atopic dermatitis, psoriasis, facial redness, folliculitis, dry skin, itchy skin, and hyperpigmentation.

4. 1. A method for treating a skin condition or skin disorder, comprising topically administering to a subject a composition comprising an effective amount of an in vitro grown red grape cell culture and a cosmetically or pharmaceutically acceptable carrier.

5. The method according to any one of claims 1 to 3, wherein the composition is in a form for oral administration.

6. The method of claim 4 , wherein the composition is in a form for topical administration.

7. 7. The method of any one of claims 1 to 6, wherein the in vitro grown red grape cell culture is grown in a bioreactor.

8. 8. The method of any one of claims 1 to 7, wherein the composition is in the form of a powder comprising a cell line culture of grapevine cells grown in vitro in a large scale scale-up process, the cell line culture of grapevine cells being derived from one or more of grape berry cross sections, grape berry skin, grape berry pulp, grape seeds, grape embryos of seeded or seedless cultivars, or grape seed skins, and the cell line culture of grapevine cells comprises resveratrol in an amount of at least 1000 mg / kg powder.

9. The method of any one of claims 1 to 8, wherein the composition is or comprises Vinia®.

10. A topical composition for treating the skin or scalp, comprising an effective amount of an in vitro grown red grape cell culture and a cosmetically or pharmaceutically acceptable carrier.

11. 11. The topical composition of claim 10, wherein the in vitro grown red grape cell culture is grown in a bioreactor.

12. 12. The composition of claim 10 or 11, wherein the in vitro grown red grape cell culture is in the form of a powder comprising a cell line culture of grape cells grown in vitro in a large scale scale-up process, the cell line culture of grape cells being derived from one or more of grape berry cross sections, grape berry skins, grape berry pulp, grape seeds, grape embryos of seeded or seedless cultivars, or grape seed skins, and the cell line culture of grape cells comprises resveratrol in an amount of at least 1000 mg / kg powder.

13. 13. The composition of any one of claims 4 and 6 to 12, wherein the composition is in the form of a cream, a facial wash or shampoo, a soap, a cleanser, an antiperspirant, a deodorant, a sunscreen, an astringent, a lotion, a moisturizer, a serum, a mask, a face or body cosmetic, an ointment, a lotion, a gel, a foam, a solution, or a powder.

14. 1. A composition comprising an in vitro grown red grape cell culture and a pharmaceutically or food acceptable carrier for treating a skin disease, condition or disorder in a subject in need thereof.

15. A composition comprising an in vitro grown red grape cell culture for treating a skin disease, condition or disorder in a subject in need thereof.

16. 16. The composition of claim 14 or 15, wherein the skin disease, condition or disorder is one or more of acne, atopic dermatitis, psoriasis, facial redness, folliculitis, dry skin, or itchy skin.

17. The composition according to any one of claims 14 to 16, wherein the composition is in a dosage form suitable for oral administration.

18. 18. The composition of any one of claims 14 to 17, wherein the in vitro grown red grape cell culture is grown in a bioreactor.

19. 19. The composition of any one of claims 14 to 18, wherein the in vitro grown red grapevine cell culture is in the form of a powder comprising a cell line culture of grapevine cells grown in vitro in a large scale up process, the cell line culture of grapevine cells being derived from one or more of grape berry cross sections, grape berry skin, grape berry pulp, grape seeds, grape embryos of seeded or seedless cultivars, or grape seed skins, and the cell line culture of grapevine cells comprises resveratrol in an amount of at least 1000 mg / kg powder.

20. The composition of any one of claims 14 to 19, wherein the composition is or comprises Vinia®.

21. 1. A cosmetic composition comprising an in vitro grown red grape cell culture and a cosmetically acceptable carrier for treating, reducing, alleviating, mitigating, or eliminating red grape skin in a subject in need thereof.

22. 22. A cosmetic composition according to claim 21 for treating, reducing, alleviating, mitigating or eliminating skin aging, wrinkles, facial redness, hyperpigmentation and skin blemishes.