How to deodorize space
The use of benzyl alcohol in a space deodorizer addresses the limitations of existing deodorizers by effectively eliminating a variety of odors and pathogens in large spaces through diffusion, ensuring safety and stability.
Patent Information
- Application Number
- JP2020217182
- Authority / Receiving Office
- JP · JP
- Patent Type
- Patents
- Current Assignee / Owner
- Filing Date
- 2020-12-25
- Publication Date
- 2025-06-05
- Estimated Expiration
- 2040-12-25
AI Technical Summary
Existing deodorizers are ineffective in addressing a wide range of odors in large spaces, have limitations in odor masking fragrances, and may use hazardous chemicals, making them unsuitable for spraying into air or treating entire rooms effectively.
A space deodorizer using benzyl alcohol as a deodorizing active ingredient, dispersed into the air or impregnated into a holder, which can be diffused through an aerosol container or a device with a fan, effectively eliminating various malodors by diffusing benzyl alcohol at concentrations of 0.05 ppm or more.
Benzyl alcohol effectively eliminates malodors such as ammonia, isovaleric acid, diacetyl, trimethylamine, mercaptan, trichloroanisole, and nonenal, while also inactivating viruses and bacteria, providing a safe and stable deodorizing solution for various spaces.
Smart Images

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Abstract
Description
[Technical field]
[0001] The present invention relates to a space deodorizer and a space deodorizing method for deodorizing, for example, a room. [Background technology]
[0002] Various types of space deodorizers have been known for the past, which are used to deodorize spaces such as rooms, etc. The deodorizing mechanisms are also varied, including those that deodorize by adsorbing malodorous components, those that deodorize by a masking effect, and those that chemically deodorize by utilizing a chemical reaction.
[0003] Furthermore, Patent Document 1 discloses a method for weakening the odor intensity of a solute by directly contacting the solute with a solvent, in which the value obtained by subtracting the solubility parameter value of the solute from the solubility parameter value of the solvent is within the range of −4 to +16. In this method, the odor is weakened by contacting the odor with a solvent having a solubility parameter value close to the solubility parameter value of the odor component. [Prior art documents] [Patent documents]
[0004] [Patent Document 1] JP 2002-238986 A Summary of the Invention [Problem to be solved by the invention]
[0005] However, there are many causes of bad odors in the home, and there is a demand for deodorizers that can deal with all the bad odors in the home at once. However, each of the existing deodorizers has its own advantages and disadvantages, and there is no known deodorizer that can properly deal with all the bad odors in the home at once.
[0006] For example, a deodorizer that eliminates odors by adsorbing malodorous components can be expected to be effective in eliminating odors in a small space, but cannot be expected to be sufficiently effective in eliminating odors in a large space such as an entire room.
[0007] In addition, masking deodorants can be sprayed over the entire space of a room, but because they have their own smell, which users may like or dislike, and the optimal masking fragrance varies for each odor component, so even if the entire room is sprayed, there are only a limited number of cases where the desired effect can be obtained.
[0008] Furthermore, chemical deodorizers often use highly reactive compounds and are therefore not suitable for spraying into air. Also, the malodorous components that can be deodorized are limited to certain chemical species, so they are not designed to treat the entire space of a room.
[0009] On the other hand, Patent Document 1 points out that the odor control agent can be sprayed into space, but the deodorizing effect has only been confirmed when it is sprayed directly onto the source of the malodor, and it is unclear whether it has a deodorizing effect when sprayed into space (when not sprayed directly onto the source of the malodor). Patent Document 1 also confirms the deodorizing effect of spraying benzyl alcohol directly onto the source of the malodor component pyridine, for example, but the deodorizing effect on other malodor components is unclear.
[0010] The present invention has been made in view of the above-mentioned points, and an object of the present invention is to provide a space deodorizer and a space deodorizing method capable of simultaneously deodorizing various malodors in a space. [Means for solving the problem]
[0011] In order to achieve the above object, the first disclosure is a space deodorizer characterized by containing benzyl alcohol as a deodorizing active ingredient.
[0012] The second disclosure is a method for deodorizing space, which is characterized by diffusing benzyl alcohol into space as a deodorizing active ingredient.
[0013] According to this configuration, it is possible to eliminate various malodors in the home by dispersing benzyl alcohol as a deodorizing active ingredient into the air. Examples of malodor components that can be eliminated by benzyl alcohol include ammonia, isovaleric acid, diacetyl, trimethylamine, mercaptan, trichloroanisole, and nonenal.
[0014] Benzyl alcohol is a compound that exists naturally as a precursor to the scent of jasmine and other fragrances, and is also used as a food additive. It is highly safe and stable as a compound, so it can be released into the air at a stable concentration.
[0015] Furthermore, according to this configuration, it is not necessary to directly contact the source of the bad odor with benzyl alcohol, but it is sufficient to simply disperse it into the space, so that even if the source of the bad odor is unknown, the entire space can be deodorized.
[0016] In the third disclosure, the benzyl alcohol is sprayed into space from an aerosol container containing the benzyl alcohol together with a propellant. With this configuration, it is possible to spray the benzyl alcohol into space with force and to diffuse it over a wide range.
[0017] In the fourth disclosure, the benzyl alcohol is impregnated into an impregnated body, and the benzyl alcohol is diffused into space by blowing air onto the impregnated body. According to this configuration, the benzyl alcohol can be continuously diffused into space for a long period of time, and the effect of the benzyl alcohol can be continuously obtained.
[0018] In the fifth disclosure, the benzyl alcohol can be diffused into space so that the concentration of the benzyl alcohol in the air is 0.05 ppm or more, thereby further enhancing the deodorizing effect. Effect of the Invention
[0019] As described above, by dispersing benzyl alcohol as a deodorizing active ingredient into a space, various bad odors in the space can be eliminated all at once. [Brief description of the drawings]
[0020] [Figure 1] FIG. 1 is a perspective view of a spatial deodorizing device according to a first embodiment of the present invention, as viewed from above. [Diagram 2] FIG. 2 is a vertical cross-sectional view of the space deodorizing device. [Diagram 3] FIG. 2 is a perspective view of the cartridge as viewed from above. [Figure 4] FIG. 1 is a perspective view of an aerosol product according to a second embodiment of the present invention. [Diagram 5] 1 is a graph showing the deodorizing effect on ammonia. [Figure 6] 1 is a graph showing the deodorizing effect on isovaleric acid. [Figure 7] 1 is a graph showing the deodorizing effect on diacetyl. [Figure 8] 1 is a graph showing the deodorizing effect against trimethylamine. [Figure 9] 1 is a graph showing the deodorizing effect on mercaptan. [Figure 10] 1 is a graph showing the spatial contact effect against influenza viruses. [Figure 11] 1 is a graph showing the spatial contact effect against feline calicivirus. [Figure 12] 1 is a graph showing the direct contact effect against influenza virus. [Figure 13] 1 is a graph showing the direct contact effect against feline calicivirus. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS
[0021] Hereinafter, the preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings. Note that the following description of the preferred embodiments is merely illustrative in nature and is not intended to limit the present invention, its applications, or its uses.
[0022] (Embodiment 1) 1 is a perspective view of a space deodorizing device 1 according to a first embodiment of the present invention, seen from above. The shape and structure of the space deodorizing device 1 of the first embodiment are merely examples, and are not limited to the shape and structure shown in the drawing.
[0023] The space deodorizing device 1 comprises a cartridge 2 (shown in Figs. 2 and 3) and a device body 5 to which the cartridge 2 is detachably attached. In the explanation of this embodiment 1, for the sake of convenience, one side in the longitudinal direction of the space deodorizing device 1 is referred to as the front side, and the other side in the longitudinal direction is referred to as the rear side, as shown in each drawing. The side that is right when the space deodorizing device 1 is viewed from the front side is referred to as the right side, and the side that is left side is referred to as the left side.
[0024] First, as shown in Fig. 2 and Fig. 3, the configuration of the cartridge 2 will be described. The cartridge 2 includes a case 3 and a liquid agent holder (impregnated body) 4 that holds a liquid agent (space deodorant) containing benzyl alcohol as a deodorizing active ingredient. The case 3 is a container that holds the liquid agent holder 4. As a liquid agent held in the liquid agent holder 4, a space deodorant containing benzyl alcohol as a deodorizing active ingredient that has the property of volatilizing at room temperature can be given. The space deodorant may be held by impregnating the liquid agent holder 4, or may be held by adhering to the surface of the liquid agent holder 4. The space deodorant may contain an ingredient other than benzyl alcohol.
[0025] The liquid agent holder 4 is made of a pleated cloth material that is folded to form a continuous wave shape. The cloth material may be, for example, a nonwoven fabric or a woven fabric, but is not particularly limited as long as it has breathability and can hold the liquid agent (such as a breathable foam material). The liquid agent is held almost uniformly over the entire liquid agent holder 4.
[0026] An air circulation hole 3a is formed in the upper wall of the case 3. In addition, an air circulation hole 3b is also formed in the lower wall of the case 3, as shown in FIG.
[0027] (Configuration of device main body 5) The configuration of the device body 5 will be described with reference to Figures 1 and 2. The device body 5 has a base member 30, a fan 50 as a blower, an electric motor 51 that drives the fan 50, and a battery (not shown) that supplies power to the electric motor 51. The fan 50, the electric motor 51, and the battery are accommodated in the base member 30 of the device body 5.
[0028] That is, the base member 30 is formed by molding, for example, a resin material. An electric motor 51 is fixed to the approximate center of the base member 30. A center portion of a fan 50 is fixed to an output shaft 51a of the electric motor 51 so as to rotate integrally with the output shaft 51a. The fan 50 is a well-known type, and when driven to rotate by the electric motor 51, it draws in air from above and blows it out from the side.
[0029] A cartridge accommodating space R for exchangeably accommodating a cartridge 2 including a liquid agent holder 4 is formed above the fan 50. This cartridge accommodating space R is a space formed by an apparatus main body 5 and a cover 40 attached to the apparatus main body 5.
[0030] 2, liquid agent diffusion holes 55 for discharging air sent to the liquid agent holder 4 by the fan 50 are formed on both the left and right sides of the base member 30 of the device body 5. The upstream end of the liquid agent diffusion hole 55 communicates with the space in the device body 5 in which the fan 50 is disposed. The air that has flowed through the liquid agent diffusion hole 55 tends to flow toward the outside of the space deodorizing device 1 as shown by arrow A.
[0031] 1, the cover 40 is made of, for example, a molded resin material, and is formed so as to cover from above the cartridge accommodating space R. The cover 40 is formed with an air circulation hole 40a.
[0032] (Operation of space deodorizer 1) Next, the operation of the space deodorizing device 1 will be described. When the power supply of the space deodorizing device 1 is turned on, the electric motor 51 is operated. When the electric motor 51 is operated, the fan 50 is rotated and driven, and air is sucked in from above the fan 50 and discharged from the side. Due to this air flow, the air above the cover 40 is sucked into the cartridge storage space R from the air circulation hole 40a of the cover 40 as shown by the arrow B in FIG. 2. Since the cartridge storage space R contains the cartridge 2, the sucked air flows toward the cartridge 2 and flows into the inside of the case 3 from the inflow opening 3a formed in the upper part of the case 3 of the cartridge 2. The air that flows into the inside of the case 3 flows out to the outside of the case 3 from the air circulation hole 3b formed in the lower wall part of the case 3. The air containing the liquid agent that flows out to the outside of the case 3 flows into the left and right liquid agent diffusion holes 55, 55 of the device main body 5, respectively, and then is diffused into the external space from each liquid agent diffusion hole 55 as shown by the arrow A in FIG. 2.
[0033] The configuration of the space deodorizing device is not limited to this. For example, the space deodorizing device may be configured with only the cartridge 2. In this case, the space deodorizing agent can be diffused and spread in the space by natural convection of air, rather than by forcibly sending air. Also, the case 3 of the cartridge 2 may be omitted, and the space deodorizing agent may be diffused and spread in the space by only the holder that holds the space deodorizing agent.
[0034] (Embodiment 2) FIG. 4 is a perspective view of an aerosol product 100 for deodorizing space according to a second embodiment of the present invention. The aerosol product 100 includes a space deodorizer containing benzyl alcohol as a deodorizing active ingredient, a propellant, an aerosol container 101 containing the space deodorizer and the propellant, a spray button 102, and a spray valve 103. The space deodorizer may contain components other than benzyl alcohol. The propellant is for spraying the space deodorizer contained in the aerosol container 101 into space, and is, for example, liquefied petroleum gas (LPG) or dimethyl ether (DME). Only one of liquefied petroleum gas and dimethyl ether may be used, or they may be mixed in any ratio.
[0035] The aerosol container 101 may be a metal container having pressure resistance that has been known in the past. An injection valve 103 is attached to the top of the aerosol container 101. The injection valve 103 is operated by an injection button 102, and has a discharge pipe (not shown) for moving in the vertical direction and discharging the contents contained in the aerosol container 101, a biasing member (not shown) made of a spring or the like for biasing the discharge pipe upward, and a valve body (not shown). The discharge pipe is in a position extending in the vertical direction, and when it is at the uppermost position, the lower end (upstream end) of the discharge pipe is closed by the valve body so that the inside of the aerosol container 101 and the discharge pipe are not in communication, whereas when the discharge pipe is pushed downward from the uppermost position, the lower end of the discharge pipe is opened so that the inside of the aerosol container 101 and the discharge pipe are in communication and the contents are discharged from the upper end of the discharge pipe. This valve mechanism is configured as described above.
[0036] The spray button 102 is formed with a spray nozzle 102a that communicates with the discharge pipe. The spray button 102 is also provided with a locking mechanism (not shown) that fixes the spray button 102 in position when the spray button 102 is pressed down. Therefore, when the spray button 102 is pressed down, the discharge pipe is fixed in the pressed down state, and the lower end of the discharge pipe is held in an open state, so that the entire amount of the content is sprayed from the spray nozzle 102a. In other words, this aerosol container 101 is a total amount spray type.
[0037] In addition, the container may not be a full-amount spray type, but may be a general aerosol container. In other words, the container may be configured so that benzyl alcohol is sprayed only while the spray button is pressed down, and spraying of benzyl alcohol stops when the spray button is released. However, from the viewpoint of preventing excessive spraying of benzyl alcohol, an aerosol container having a fixed amount is preferable, and in this respect, the aerosol container 101 may be a fixed amount spray type that sprays a fixed amount at one time. In this case, for example, a container described in JP 2014-28631 A may be used.
[0038] (Deodorizing method) Next, a method for deodorizing space will be described. The method for deodorizing space can be performed using the space deodorizing device 1 of the first embodiment, and can also be performed using the aerosol product 100 of the second embodiment. Target spaces include, for example, rooms in ordinary homes, offices, stores, classrooms, various event spaces, interiors of vehicles, interiors of aircraft, interiors of ships, etc., but other spaces can also be targeted.
[0039] That is, when the spatial deodorizer 1 of the first embodiment is used, benzyl alcohol can be diffused into the space as a deodorizing active ingredient by operating the spatial deodorizer 1. The amount of spatial deodorizer diffused can be adjusted by the amount of spatial deodorizer held in the liquid agent holder 4. The amount of spatial deodorizer diffused per unit time can be adjusted by the amount of spatial deodorizer held in the liquid agent holder 4, the amount of air blown by the fan 50, the surface area of the liquid agent holder 4, etc.
[0040] In the case of the aerosol product 100 of the second embodiment, benzyl alcohol can be diffused into the space as a deodorizing active ingredient by pressing the spray button 102. The amount of the spatial deodorant diffused can be adjusted by the amount of the spatial deodorant contained in the aerosol container 101. The amount of the spatial deodorant diffused per unit time can be adjusted by the flow rate of the spray valve 103, the nozzle diameter of the spray nozzle 102a, etc.
[0041] In both the first and second embodiments, it is preferable to close the doors and windows of the room before dispersing the spatial deodorant into the space, but the doors and windows of the room may be closed immediately after dispersing the spatial deodorant into the space. In addition, it is preferable to ventilate the space after a predetermined time has elapsed after dispersing the spatial deodorant into the space, but ventilation is not necessary if the airborne concentration of benzyl alcohol is extremely low. In both the first and second embodiments, the amount of the spatial deodorant to be dispersed can be set according to the size of the space to be treated, and the amount of the spatial deodorant to be dispersed per unit time can be set. This allows the airborne concentration of benzyl alcohol in the space to be treated to be adjusted to an appropriate range.
[0042] The upper limit of the airborne concentration of benzyl alcohol in the space to be treated is set in consideration of its effect on the human body. In the case of a formulation that constantly dissipates (embodiment 1), it is necessary to take into consideration chronic toxicity, and in this case, it is preferable to set the amount of benzyl alcohol emitted so that the airborne concentration of benzyl alcohol is 5.565 ppm or less, preferably 5.00 ppm or less. On the other hand, in the case of a formulation that is used once and is assumed to be ventilated after use, such as a total-amount spray aerosol (embodiment 2), single-use toxicity may be sufficient, and in this case, it is preferable to set the amount of benzyl alcohol emitted so that the airborne concentration is 2000 ppm or less, preferably 1500 ppm or less.
[0043] In both the first and second embodiments, it is preferable to set the amount of benzyl alcohol emitted so that the concentration of benzyl alcohol in the air is 0.05 ppm or more. This allows various odors in the home to be deodorized. Examples of various odorous components in the home include ammonia, isovaleric acid, diacetyl, trimethylamine, mercaptan, trichloroanisole, and nonenal. The air deodorizer according to this embodiment and the air deodorizing method using the same have a high deodorizing effect on ammonia, isovaleric acid, diacetyl, trimethylamine, mercaptan, trichloroanisole, and nonenal. The present invention can also be called an odor component reducer and an odor component reduction method that remove or reduce odorous components contained in the air. EXAMPLES
[0044] Next, examples of the present invention will be described, but the present invention is not limited to these examples.
[0045] (Test to confirm deodorizing effect against ammonia) In this test, an air deodorizer containing benzyl alcohol as an active ingredient was evaporated into the air, and the deodorizing effect against the malodorous components in the air was confirmed. In other words, the effectiveness of benzyl alcohol was confirmed when it was diffused into the air in the form of vapor, not in the form of liquid.
[0046] First, a test to confirm the deodorizing effect of the air deodorizer on ammonia, one of the various odors found in the home, will be described. 1. Prepare a glass cylinder with a volume of 10 L and close the top end of the glass cylinder with a glass lid, and also close the bottom end in the same way. 2. A petri dish containing filter paper (ADVANTEC, No5A, 90mm) was placed inside the glass cylinder. Ammonia was applied to the filter paper. Ammonia was prepared as a 0.4% aqueous solution, and 0.25mL of the solution was applied. 3. Wait until the glass cylinder is filled with ammonia, and then remove the filter paper from the glass cylinder. 4. A specified amount of the test substance is applied to another filter paper and placed in the glass cylinder. The test substance is an air deodorant (benzyl alcohol). 0.0022 mg of benzyl alcohol is applied to the filter paper. When the entire amount evaporates, the air concentration of benzyl alcohol in the glass cylinder becomes 0.05 ppm. 5. The filter paper coated with the test substance was placed in the glass cylinder and the time elapsed was measured immediately after the lid was closed, and the concentration of the malodorous components in the air inside the glass cylinder was measured using a detector tube at regular intervals. The detector tube was a Gastec Gas Detector Tube No. 36L Ammonia.
[0047] The measurement results are shown in the graph in Figure 5. The horizontal axis of the graph in Figure 5 is the elapsed time, and the vertical axis is the malodor removal rate (malodor removal rate by the test agent) compared to the blank. The blank is the case where the filter paper coated with the test agent was not placed in the glass cylinder in step 4. The malodor removal rate A was calculated using the following formula.
[0048] A = (Blank measurement value - Measurement value when test substance is used) / Blank measurement value x 100 (%)
[0049] As shown in the graph, the odor removal rate of the test agent was 63% after 30 minutes, 67% after 1 hour, 80% after 2 hours, and 100% after 3 hours. After applying the agent to the space inside the glass cylinder, almost all of the ammonia was removed from inside the glass cylinder after 3 hours. The criteria set by the Aromatic Deodorizer Council state that a deodorizing effect is observed if a similar test (using an airbag instead of a glass cylinder) removes 90% of the odorous components within 10 hours. In this case, 100% was removed after 3 hours, so in light of the above criteria, it can be said that the agent had a deodorizing effect against ammonia.
[0050] (Deodorizing effect confirmation test for isovaleric acid) Next, we will explain the deodorizing effect confirmation test of the air freshener on isovaleric acid, one of the various unpleasant odors found in the home. The test system was the same as the ammonia test. The difference is that in step 2, isovaleric acid was applied to the filter paper. Isovaleric acid was prepared as a 0.4% aqueous solution, and 0.3 mL of that solution was applied. The detector tube used to measure the concentration in step 5 was a "Gastec Gas Detector Tube No. 81L Acetic Acid."
[0051] The measurement results are shown in the graph in Figure 6. The horizontal axis of the graph in Figure 6 is the elapsed time, and the vertical axis is the malodor removal rate (malodor removal rate by the test agent) compared to the blank. The blank was the case where the filter paper coated with the test agent was not placed in the glass cylinder in step 4. The formula for calculating the malodor removal rate is the same as for the ammonia test.
[0052] As shown in the graph, the odor removal rate of the test agent was 13% after 1 hour, 38% after 2 hours, 62% after 3 hours, 87% after 4 hours, and 100% after 5 hours. After treatment into the space inside the glass cylinder, almost all of the isovaleric acid was removed from within the glass cylinder after 5 hours, so it can be said that the agent had a deodorizing effect.
[0053] (Deodorizing effect confirmation test for diacetyl) Next, we will explain the deodorizing effect confirmation test of the air freshener on diacetyl, one of the various unpleasant odors found in the home. The test system was the same as the ammonia test. The difference is that in step 2, diacetyl was applied to the filter paper. A 0.1% solution of diacetyl was diluted with ion-exchanged water, and 300 μL was applied. The detector tube used was a "Gastec Gas Detector Tube for Acetaldehyde."
[0054] The measurement results are shown in the graph in Figure 7. The horizontal axis of the graph in Figure 7 is the elapsed time, and the vertical axis is the malodor removal rate (malodor removal rate by the test agent) compared to the blank. The blank was obtained when the glass cylinder was filled with diacetyl as described above, and then the filter paper coated with the test agent was not placed in the glass cylinder. The formula for calculating the malodor removal rate is the same as that for the ammonia test.
[0055] As shown in the graph, the odor removal rate of the test agent was 50% after 30 minutes and 100% after 1 hour. After treatment into the space inside the glass cylinder, almost all of the diacetyl was removed from within the glass cylinder after 1 hour, so it can be said that the agent had a deodorizing effect.
[0056] (Test to confirm deodorizing effect against trimethylamine) Next, we will explain the deodorizing effect confirmation test of the air freshener on trimethylamine, one of the various unpleasant odors found in the home. The test system was the same as the ammonia test. The difference is that in step 2, trimethylamine was applied to the filter paper. A 0.1% solution of trimethylamine was diluted with ion-exchanged water, and 300 μL was applied. The detector tube used to measure the concentration in step 5 was a "Gastec Gas Detector Tube for Amines."
[0057] The measurement results are shown in the graph in Figure 8. The horizontal axis of the graph in Figure 8 is the elapsed time, and the vertical axis is the malodor removal rate (malodor removal rate by the test agent) compared to the blank. The blank was obtained when the glass cylinder was filled with trimethylamine as described above, and then the filter paper coated with the test agent was not placed in the glass cylinder. The formula for calculating the malodor removal rate is the same as for the ammonia test.
[0058] As shown in the graph, the odor removal rate of the test agent was 50% after 1 hour, 2 hours, and 3 hours, 75% after 4 hours and 5 hours, 85% after 6 hours, and 90% after 7 hours. After applying the agent to the space inside the glass cylinder, most of the trimethylamine was removed from within the glass cylinder after 7 hours, so it can be said that the agent had a deodorizing effect.
[0059] (Deodorizing effect confirmation test against mercaptan) Next, we will explain the deodorizing effect confirmation test of the air freshener on mercaptan, one of the various unpleasant odors in the home. The test system was the same as the ammonia test. The difference is that in step 2, 200μL of mercaptan standard solution was applied to the filter paper. The detector tube used to measure the concentration in step 5 was the "Gastec Gas Detector Tube Mercaptan."
[0060] The measurement results are shown in the graph in Figure 9. The horizontal axis of the graph in Figure 9 is the elapsed time, and the vertical axis is the malodor removal rate (malodor removal rate by the test agent) compared to the blank. The blank was obtained when the glass cylinder was filled with mercaptan as described above, and then the filter paper coated with the test agent was not placed in the glass cylinder. The formula for calculating the malodor removal rate is the same as for the ammonia test.
[0061] As shown in the graph, the odor removal rate of the test agent was 40% after 1 hour, 44% after 2 hours, 50% after 3 and 4 hours, 60% after 5 hours, 65% after 6 hours, and 80% after 7 hours. After 7 hours, most of the mercaptan was removed from the glass cylinder. When other deodorants (commercially available deodorants made of highly absorbent polymers and green tea extract, a known deodorant ingredient) were used in the same deodorant test against mercaptan, the removal rate after 7 hours was 60% or less in both cases. Therefore, it can be said that benzyl alcohol shows an effective deodorant effect against mercaptan compared to other deodorant ingredients.
[0062] (Deodorizing effect confirmation test for trichloroanisole) Next, we will explain the deodorizing effect confirmation test of the air deodorizer against trichloroanisole (2,4,6-trichloroanisole), one of the various odors in the home. Since we could not obtain a suitable detector tube to measure the concentration of trichloroanisole, we confirmed the deodorizing effect by a sensory test. 1. Prepare a glass cylinder with a volume of 10 L and close the top end of the glass cylinder with a glass lid, and also close the bottom end in the same way. 1 mL of 2.4 μg / mL trichloroanisole in 99% ethanol is dropped onto the filter paper, and the filter paper is placed into the glass cylinder. 3. Wait until the glass cylinder is filled with trichloroanisole, and then remove the filter paper from the glass cylinder. 4. A specified amount of the test substance is applied to another filter paper and placed in the glass cylinder. The test substance is an air deodorant (benzyl alcohol). 0.0022 mg of benzyl alcohol is applied to the filter paper. When the entire amount evaporates, the air concentration of benzyl alcohol in the glass cylinder becomes 0.05 ppm. 5. The filter paper coated with the test substance was placed in the glass cylinder, and the odor inside the glass cylinder was checked by sensory analysis one hour after the lid was closed. The concentration of trichloroanisole was set based on the TCA concentration in the off-flavor discrimination ability measurement method of the sensory panel of the Tokyo Kasei University Off-Flavor Research Group.
[0063] A blank was also prepared, in which the filter paper coated with the test agent was not placed in the glass cylinder in step 4.
[0064] The sensory evaluation was carried out on 15 subjects. Each subject was asked to rate the blank and the test agent from 0 to 5, with "0" representing no odor, "1" representing a barely detectable odor, "2" representing a weak odor that was easy to identify, "3" representing a strong odor, and "5" representing an overwhelming odor. As a result, the average score of the 15 subjects was 2.33 for the blank, while it was 1 for the test agent. According to the Deodorizer Efficacy Test Method of the Japan Association of Deodorizers, the odor intensity was reduced by at least one level compared to the blank, so it can be said that the test agent has a high deodorizing effect.
[0065] (Deodorizing effect confirmation test against nonenal) Next, we will explain the deodorizing effect confirmation test of the air deodorizer against nonenal, one of the various odors in the home. The test system is the same as that for trichloroanisole. The difference is that in step 2, 0.4 mL of 0.4% nonenal in 99% ethanol was dropped onto the filter paper.
[0066] The method of sensory evaluation was the same as for nonenal. As a result of the sensory evaluation, the average score of 15 people was 4.33 for the blank, while it was 3.2 for the test agent. According to the Deodorizer Efficacy Test Method of the Japan Association of Deodorizers, the odor intensity was reduced by more than one level compared to the blank, so it can be said that the test agent has a high deodorizing effect.
[0067] (Virus inactivation test by spatial contact) The inventors of the present invention have confirmed through their research that benzyl alcohol, which is the active ingredient of the air deodorizer of the present invention, has a virus inactivation effect in air. This virus inactivation effect will be described below.
[0068] In this test, an air freshener containing benzyl alcohol as its active ingredient was evaporated into the air, and its effectiveness in inactivating viruses on the surfaces of objects within the air was confirmed. 1. A glass cylinder with a volume of 10 L is placed, and the desired amount of benzyl alcohol (air deodorant) in a glass petri dish is placed in the glass cylinder. The amount of benzyl alcohol is 0.002 mg. When the entire amount of benzyl alcohol evaporates, the air concentration of benzyl alcohol in the glass cylinder will be 0.05 ppm. Also, by setting the amount of benzyl alcohol to 0.0009 mg, the air concentration of benzyl alcohol in the glass cylinder will be 0.02 ppm. 2. Pour a saturated aqueous solution of humidity control into the glass cylinder. 3. Apply 50 μL of virus to a 2 cm x 2 cm gold cloth. 4. The gold cloth is placed in the glass cylinder and exposed to air containing evaporated benzyl alcohol for 30 minutes or 1 hour. 5. After contact, remove the gold cloth from the glass cylinder and place it in a microtube. 6. Add 350 μL of PBS (phosphate buffer solution) to the metal cloth so that it penetrates the cloth, and tap the microtube to move the PBS up and down. 7. Use heated tweezers to make a hole in the bottom of the microtube containing the gold cloth and PBS, and place another microtube underneath it. Centrifuge the two stacked microtubes at 25℃ and 10,000 rpm for 1 minute. 8. Prepare 8 microtubes (number of samples) and dispense 450 μL of DMEM into each tube. 9. After centrifugation, take 50 μL of the virus solution that has fallen into the lower microtube and dilute it by adding it to one of the microtubes with DMEM dispensed in. Take 50 μL of the diluted virus solution and dilute it in 8 steps as described above. 10. Culturing: Prepare a culture plate on which each cell has been cultured, and remove the culture medium from each well using an aspirator. 11. Dispense 50 μL of the virus solution diluted in 8 steps into each well. 12.Incubate in a 37°C, 5% carbon dioxide incubator for 60 minutes. 13. After the reaction, remove the virus solution and inject 100 μL of DMEM into each well and culture for 4 days.
[0069] At this time, in the case of influenza virus, pour the mixture into each well so that the mixture becomes DMEM 100 μL / well + 30 μg / mL trypsin. 14. Pour about 100 μL of ethanol:acetic acid = 4:1 (weight ratio) into each well to immobilize the antibodies for about 40 minutes. 15. After removing the fixative, add 30 μL of 0.5% amido black in 45% ethanol and 10% acetic acid to stain the cells. 16. Immerse the plate in a container filled with water to wash off the staining solution without damaging the cells. 17. Virus infectivity TCID 50 asked for.
[0070] The above steps (steps 1 to 17) were performed with influenza virus and feline calicivirus. A blank was also prepared in which benzyl alcohol was not added in step 1, and no spatial contact between the virus and benzyl alcohol was performed in step 4. Test systems were also prepared in which the concentration of benzyl alcohol in the glass cylinder in step 1 was changed to three levels: 0.02 ppm, 0.05 ppm, and 0.1 ppm, and tests were performed with influenza virus and feline calicivirus for each.
[0071] The results of the test using influenza virus are shown in the graph in Figure 10. In step 4 above, the influenza virus was exposed to spatial contact with benzyl alcohol for 30 minutes and for 1 hour, with the left side of the graph showing the result of spatial contact for 30 minutes and the right side of the graph showing the result of spatial contact for 1 hour. Even with a benzyl alcohol concentration of 0.02 ppm, the influenza virus was inactivated, but with a benzyl alcohol concentration of 0.05 ppm or more, the influenza virus could be inactivated by 99% or more after 1 hour of spatial contact.
[0072] The results of the test using feline calicivirus are shown in the graph in Figure 11. Even if the benzyl alcohol concentration is 0.02 ppm, spatial contact for 1 hour is effective in inactivating the feline calicivirus, but if the benzyl alcohol concentration is 0.05 ppm or higher, spatial contact for 1 hour can inactivate 99% or more of the feline calicivirus.
[0073] (Antiviral effect duration test) At least a portion of the deodorant (benzyl alcohol) diffused into the space adheres to the surface of objects in the space. This test was conducted under the assumption that the space was treated with benzyl alcohol, and then the benzyl alcohol adhered to the surface of objects in the space, and confirmed how long the antiviral effect on the surface of the object would last after the benzyl alcohol adhered to it. A specified amount of benzyl alcohol is applied to a 1.2 x 2 cm gold cloth and left to stand in an open space for a specified number of days (3 days, 7 days, 14 days, or 21 days). 2. After the specified number of days have passed, apply 50 μL of influenza virus or feline calicivirus to the gold cloth and allow it to come into direct contact for 2 hours (ISO standard). 3. After contact, place the gold cloth into a microtube. 4. Add 350 μL of PBS (phosphate buffer solution) to the metal cloth so that it penetrates the cloth, and tap the microtube to move the PBS up and down. 5. Use heated tweezers to make a hole in the bottom of the microtube containing the gold cloth and PBS, and place another microtube underneath it. Centrifuge the two stacked microtubes at 25℃ and 10,000 rpm for 1 minute. 6. Prepare 8 microtubes (number of samples) and dispense 450 μL of DMEM into each tube. 7. After centrifugation, take 50 μL of the virus solution that has fallen into the lower microtube and dilute it by adding it to one of the microtubes with DMEM. Take 50 μL of the diluted virus solution and dilute it in 8 steps as described above. 8. Prepare a culture plate on which cultured cells have been cultured, and remove the culture medium from each well using an aspirator. 9. Dispense 50 μL of the virus solution diluted in 8 steps into each well. 10.Incubate in a 37℃, 5% carbon dioxide incubator for 60 minutes. 11. After the reaction, remove the virus solution and inject 100 μL of DMEM into each well and culture for 4 days.
[0074] At this time, in the case of influenza virus, pour the mixture into each well so that the mixture becomes DMEM 100 μL / well + 30 μg / mL trypsin. 12. Pour about 100 μL of ethanol:acetic acid = 4:1 (weight ratio) into each well to immobilize the antibodies for about 40 minutes. 13. After removing the fixative, add 30 μL of 0.5% amido black in 45% ethanol and 10% acetic acid to stain the cells. 14. Immerse the plate in a container filled with water to wash off the staining solution without damaging the cells. 15. Measure the viral infectivity by standard methods. 50 asked for.
[0075] The above steps (steps 1 to 15) were performed for influenza virus and feline calicivirus. A blank was also prepared, in which benzyl alcohol was not applied in step 1.
[0076] The results of the test using influenza virus are shown in the graph in Figure 12. The tests were conducted for 3, 7, 14, and 21 days, assuming that the space was treated with an airborne concentration of 32 ppm benzyl alcohol and 49 ppm benzyl alcohol. If the space is treated with an airborne concentration of 32 ppm benzyl alcohol, for example, in a room of 3.6 m x 3.6 m x 2.4 m, and the entire amount of benzyl alcohol is assumed to adhere to the floor after treatment, approximately 1.4 μg of benzyl alcohol will adhere to the 2 cm x 2 cm area. Therefore, in the test assuming 32 ppm, the specified amount of benzyl alcohol to be applied to the gold cloth in step 1 was set to 1.4 μg. Similarly, if the space is treated with an airborne concentration of 49 ppm benzyl alcohol, 2.1 μg of benzyl alcohol will adhere to the 2 cm x 2 cm area. Therefore, in the test assuming 49 ppm, the specified amount of benzyl alcohol to be applied to the gold cloth in process 1 was set at 2.1 μg.
[0077] As shown in Figure 12, if a space is treated with benzyl alcohol at an airborne concentration of 32 ppm or more, the benzyl alcohol attached to the surfaces of objects in the space can inactivate 99.9% or more of influenza viruses for seven days after the treatment.
[0078] The test results using feline calicivirus are shown in the graph in Figure 13. As shown in Figure 13, if a space is treated with benzyl alcohol at an airborne concentration of 49 ppm or more, the benzyl alcohol attached to the surfaces of objects in the space after the treatment can inactivate 99% or more of the feline calicivirus for seven days.
[0079] (Airborne virus inactivation test) Next, an inactivation test of airborne viruses (airborne viruses) will be described. The test agent in this test is a total-aerosol containing benzyl alcohol. Specifically, the aerosol agent has the composition shown in Table 2 and is contained in an aerosol container, and the entire content is sprayed by pressing the spray button. The test space is a chamber simulating a room of about 2 tatami mats, specifically, 186 cm wide, 186 cm deep, and 186 cm high. When the entire amount of the test agent is sprayed in this test space, the airborne concentration of benzyl alcohol is 26 ppm. 1. Take out the E. coli phage stored at -40℃ in a deep freezer and thaw it at room temperature. Note that in this test, E. coli phage is used instead of influenza viruses, etc., but E. coli phage has a structure similar to that of enveloped viruses and is thought to be a substitute for influenza viruses, etc. 2. Add 10 μL of E. coli phage to 10 mL of physiological saline and mix. 3. Place the phage solution in the nebulizer and spray into the chamber for 15 minutes. 4. At almost the same time as spraying of the phage solution begins, spraying of the test agent begins (it takes several tens of seconds until the entire amount has been sprayed). 5. Collect floating phages using an impinger 15 minutes, 30 minutes, 1 hour, 2 hours, 3 hours, and 4 hours after the start of spraying. 6.1.Add 450 µL of LB liquid medium to a 5 mL sterile tube. 7. Add 50 μL of the collected phage solution to the tube and mix for 10 -5 Serial dilutions are performed up to 100 μl. 8. Transfer the E. coli preculture solution for phage to a 50 mL conical tube and centrifuge at 1500 rpm for 15 minutes. 9. After centrifugation, dilute to 10 mL. 10. Dispense 350 μL of the E. coli solution into a new 1.5 mL sterile tube. 11. Add 350 μL of the diluted phage solution to the E. coli solution dispensed in the tube to create a mixed solution. 12. Remove the LB agar medium (petri dish or LB 6-well plate) from the refrigerator and move it to a 40°C incubator. 13. Add 100 μL (if using a 6-well plate) or 200 μL (if using a petri dish) of the mixture to the LB soft agar that has been adjusted to 40°C to 43°C in a thermostatic bath. 14. Pour the entire amount of the soft agar to which the mixed liquid has been added onto the agar medium and leave it for a certain period of time until it hardens. 15. After the cells have solidified, store the petri dish or 6-well plate in a 40℃ incubator and check for plaques after 24 hours.
[0080] The test results are shown in Table 1.
[0081] [Table 1]
[0082] The blank is a chamber that is not treated with the test agent in step 4. The time in the blank is the time in step 5 above. The examples are examples in which the entire amount of the test agent was sprayed in the chamber immediately after the phage liquid was sprayed in the chamber in step 3 above. The times in the examples in Table 1 are the elapsed time from the completion of spraying of the air deodorant, and are almost the same as the time in step 4 above.
[0083] [Table 2]
[0084] As shown in Table 1, in the embodiment, 99% or more of the E. coli phages are inactivated 15 minutes after the completion of injection, and 99.99% or more of the E. coli phages are inactivated 2 hours after the completion of injection.
[0085] (Efficacy test against Staphylococcus aureus) In addition, the inventors of the present invention have confirmed that benzyl alcohol, which is an active ingredient of the air deodorant of the present invention, has a disinfecting and antibacterial effect. Next, the effectiveness test of the air deodorant against Staphylococcus aureus will be described. The test bacterium is Staphlococcus aureus NBRC12732: a gram-positive coccus. As a preliminary medium, a nutrient liquid medium (agar-free normal agar medium) is prepared and dispensed into Sakaguchi flasks at approximately 300 ml each. A cotton wire is placed in the Sakaguchi flask and sterilized in an autoclave. The liquid medium is ready for use when it has cooled to about body temperature. One platinum loop of the bacteria being subcultured is taken and inoculated into the liquid medium. The bacteria are shaken and cultured for 48 hours in a shaking incubator in the culture room. This results in a concentration of approximately 10^8 CFU / ml. The nutrient liquid medium contains 5.0 g of meat extract, 10.0 g of peptone, 5.0 g of sodium chloride, and 1000 g of ion-exchanged water per 1000 ml.
[0086] In this test, first, 10ml of bacterial suspension adjusted to 10^7CFU / ml is taken and centrifuged. The supernatant of the resulting bacterial precipitate is discarded and 1ml of physiological saline is added to prepare a bacterial solution of 10^6CFU / ml. 200μl of the bacterial solution adjusted to 10^6CFU / ml is taken and applied to a 2cm x 2cm piece of metal cloth. The metal cloth and the test agent in a plastic cup are placed separately in a 10L cylinder and allowed to react for 8 and 24 hours. The test agent is 132μl of an air freshener (benzyl alcohol), which is allowed to evaporate naturally inside the 10L cylinder.
[0087] After the reaction, the bacteria are washed off the metal cloth, diluted to 10^-1 to 10^-5 with SCDLP liquid medium, and 200 μl is plated on SCDLP agar medium. After 48 hours, the number of colonies is counted.
[0088] The test results showed that after 8 hours, the number of live bacteria in the blank was 590,000 CFU / ml, while in the case of the air freshener it was 0 CFU / ml. In other words, the sterilization rate was 99.99% or more. After 24 hours, the number of live bacteria in the blank was 105,000 CFU / ml, while in the case of the air freshener it was 0 CFU / ml. In other words, the sterilization rate was 99.99% or more.
[0089] If we assume that all of the test agent evaporated, the maximum concentration of benzyl alcohol in the air in the 10 L cylinder would be 2.9 ppm. However, since the amount of benzyl alcohol that actually evaporated was about 7.5% of the initial amount, the concentration in the air in the 10 L cylinder in this test was about 0.2 ppm.
[0090] (Effectiveness test against E. coli) Next, we will explain the effectiveness test of the air deodorizer against E. coli. The test bacteria is Escherichia coli. The preculture medium is the same as that used in the "Test against Staphylococcus aureus". However, the shaking culture in the shaking incubator is for 24 hours. This results in a concentration of approximately 10^8 CFU / ml. In this test, first, 10ml of bacterial suspension adjusted to 10^7CFU / ml is taken and centrifuged. The supernatant of the resulting bacterial precipitate is discarded and 1ml of physiological saline is added to prepare a bacterial solution of 10^6CFU / ml. 200μl of the bacterial solution adjusted to 10^6CFU / ml is taken and applied to a 2cm x 2cm metal cloth. The metal cloth and the test agent in a plastic cup are placed separately in a 10L cylinder and allowed to react for 8 and 24 hours. The test agent is 132μl of an air freshener (benzyl alcohol), which is allowed to evaporate naturally inside the 10L cylinder.
[0091] After the reaction, the bacteria are washed off the metal cloth, diluted to 10^-1 to 10^-5 with SCDLP liquid medium, and 200 μl is plated on SCDLP agar medium. After 24 hours, the number of colonies is counted.
[0092] The test results showed that after 8 hours, the number of live bacteria in the blank was 6,000,000 CFU / ml, while the number in the air deodorant was 0 CFU / ml. In other words, the sterilization rate was 99.99% or more. After 24 hours, the number of live bacteria in the blank was 6,000,000 CFU / ml, while the number in the air deodorant was 0 CFU / ml. In other words, the sterilization rate was 99.99% or more.
[0093] If we assume that all of the test agent evaporated, the maximum concentration of benzyl alcohol in the air in the 10 L cylinder would be 2.9 ppm. However, since the amount of benzyl alcohol that actually evaporated was about 7.5% of the initial amount, the concentration in the air in the 10 L cylinder in this test was about 0.2 ppm.
[0094] Under similar test conditions, the results are described below for the cases where benzyl alcohol was reacted with gold cloth for 8 hours at a maximum air concentration of 2.9 ppm (132 mg of benzyl alcohol as test agent), a maximum air concentration of 1 ppm (46.55 mg of benzyl alcohol as test agent), a maximum air concentration of 0.1 ppm (4.65 mg of benzyl alcohol as test agent), a maximum air concentration of 0.01 ppm (0.47 mg of benzyl alcohol as test agent), and a maximum air concentration of 0.001 ppm (0.047 mg of benzyl alcohol as test agent). At any of these air concentrations, the viable bacteria count was 0 CFU / ml, and the sterilization rate was 99.99% or more. In other words, a sufficient sterilization effect can be obtained if the air concentration of benzyl alcohol is at least 0.001 ppm.
[0095] (Test results when using a space deodorizing device) Next, an effectiveness test against E. coli will be explained as an effectiveness test using the spatial deodorizing device 1. The test bacteria is Escherichia coli. The preculture medium is the same as that used in the "Test against Staphylococcus aureus". In this test, first, 10 ml of a bacterial suspension adjusted to 10^7 CFU / ml is taken and centrifuged. The supernatant of the resulting bacterial precipitate is discarded and 1 ml of physiological saline is added to prepare a bacterial solution of 10^6 CFU / ml. 200 μl of the bacterial solution adjusted to 10^6 CFU / ml is taken and applied to a 2 cm x 2 cm metal cloth.
[0096] The space deodorizing device 1 was placed at the center of the bottom of a Pete Grady chamber (1.82 m long x 1.82 m wide x 1.82 m high). The liquid agent holder 4 of this space deodorizing device 1 was impregnated with 2.0 g of benzyl alcohol as a space deodorizing agent.
[0097] Metal cloths are placed at the bottom of the peat Grady chamber at a location 30 cm (location 1), 60 cm (location 2), 90 cm (location 3), and 120 cm (location 4) away from the space deodorizing device 1. In addition, metal cloths are placed on the side of the peat Grady chamber at a location 60 cm (location 5) above the bottom, 120 cm (location 6), and 180 cm (location 7) above the bottom. In this state, the fan 50 of the space deodorizing device 1 is operated for 24 hours.
[0098] The bacteria were then washed out of the gold cloth, diluted to 10^-1 to 10^-5 with SCDLP liquid medium, and 200 μl was seeded on SCDLP agar medium. The number of colonies was counted after 24 hours. In the case of the blank, the number of live bacteria was 20,025,000 CFU / ml. In contrast, the number of live bacteria was 0 CFU / ml in the first, second, third, and fourth locations. In other words, the sterilization rate was 99.99% or more. Meanwhile, the number of live bacteria was 336 CFU / ml in the fifth location, and 156 CFU / ml in the sixth location, but the sterilization rate was 99.99% or more in both cases.
[0099] After the test was completed, the air concentration of benzyl alcohol in the peat-grady chamber was calculated based on the amount of benzyl alcohol evaporated, and was found to be 0.011 ppm.
[0100] As for Staphylococcus aureus, the World Health Organization (WHO) guidelines require handling at Biosafety Level 2, which makes it difficult to test in the above-mentioned space (Pete Grady chamber). However, as shown in the above "Test for Staphylococcus aureus", by evaporating benzyl alcohol, a good sterilization effect can be obtained for Staphylococcus aureus as well as Escherichia coli. Therefore, even in the above-mentioned space (Pete Grady chamber), it can be said that a sufficient sterilization effect can be obtained for Staphylococcus aureus by evaporating benzyl alcohol using the indoor space sterilization device 1.
[0101] (Effectiveness test against E. coli) Next, the results of the efficacy test against E. coli in an open system will be explained. First, a 2cm x 2cm gold cloth is prepared as a test piece, and this gold cloth is sterilized in an autoclave. Then, a benzyl alcohol solution (132μl) is naturally evaporated and attached to the gold cloth. This gold cloth is left in an open system for 3, 7, 10, 14, 23, and 36 days. After leaving it for the specified number of days, 100μl of 1.0×10^6CFU / ml E. coli is applied to each test piece, which is then cultured for 24 hours, washed out with SCDLP liquid medium, diluted in 5 steps, and then applied to SCDLP agar medium and cultured for 24 hours. The number of colonies is then counted.
[0102] As a result, the sterilization rate of the gold cloth left to stand for 3 and 7 days after being exposed to benzyl alcohol in an open system was 99.99% or more. In other words, the antibacterial effect of benzyl alcohol is maintained for at least 7 days in an open system.
[0103] Next, we will explain the results of a similar test performed in a closed system. A 50% benzyl alcohol solution (260μl) was allowed to evaporate naturally onto a sterilized metal cloth in the same manner as above. The metal cloth was left to stand in a closed system (in a Tupperware container with the lid closed) for 3, 7, 10, 14, 23, and 36 days. After leaving the test pieces for the specified number of days, 100μl of 1.0×10^6CFU / ml E. coli was applied to each test piece, which was then cultured for 12 hours, washed out with SCDLP liquid medium, diluted in five stages, and then applied to SCDLP agar medium and cultured for 24 hours. The number of colonies was then counted.
[0104] As a result, the sterilization rate of the gold cloth left in the closed system for 3, 7, 10, 14, 23 and 36 days after the benzyl alcohol was applied was 99.99%. In other words, the antibacterial effect of benzyl alcohol is maintained for at least 36 days in a closed system.
[0105] (Antifungal test) Next, the antifungal test (test against fungi) will be explained. The test fungus is Trichophyton.
[0106] First, prepare a spore suspension. First, add 10 ml of 0.05% polysorbate 80-saline to one mold slant. Mix vigorously and thoroughly disperse the spores by scraping them off the slant with a medicine spoon or similar. Filter the spore suspension through sterile gauze (75 mm x 75 mm), and dilute the filtrate appropriately with 0.05% polysorbate 80-saline to a concentration of 10^7 spores / ml to make a single spore suspension. Repeat this process for the amount of liquid required.
[0107] Next, the contact method will be explained. A 10L cylinder is prepared, and 100μl of the adjusted bacterial suspension is applied to a 2cm x 2cm piece of gold cloth. A petri dish containing the gold cloth and 260mg of 50% benzyl alcohol are placed in the 10L cylinder, and the mixture is allowed to come into contact with the outside air for 24 hours. The test results showed that almost no mold was found in the petri dish. In addition, in the case of the blank ion-exchanged water, mold was found to have grown in about half of the area of the petri dish.
[0108] As described above, according to this embodiment, by dispersing benzyl alcohol as a deodorizing active ingredient into space, it is possible to deodorize malodors such as ammonia, isovaleric acid, diacetyl, trimethylamine, mercaptan, trichloroanisole, nonenal, etc. In addition, since it contains benzyl alcohol as an active ingredient, it is stable and a high level of safety can be ensured.
[0109] The above-described embodiment is merely illustrative in all respects and should not be construed as limiting. Furthermore, all modifications and variations within the scope of the claims are within the scope of the present invention. [Industrial Applicability]
[0110] As described above, the spatial deodorizer and method according to the present invention can be used, for example, in homes, offices, stores, and the like. [Explanation of symbols]
[0111] 1. Air deodorizer 2 Cartridge 4 Liquid agent holder (impregnated body) 50 Fans
Claims
1. A method for deodorizing an air space, comprising dispersing benzyl alcohol into the air as an effective deodorizing ingredient for at least one of isovaleric acid, diacetyl, trimethylamine, and mercaptan, and setting the air concentration of the benzyl alcohol to 0.05 ppm or more.
2. The method for deodorizing a space according to claim 1, A method for deodorizing space, comprising spraying the benzyl alcohol into space from an aerosol container containing the benzyl alcohol together with a propellant.
Citation Information
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