Compositions Comprising Spores and Pro-Flavoring Materials

Abstract

A surface treatment composition comprising about 1×10 2 ~Approx. 1×10 9 A surface treatment composition comprising: CFU / g of Bacillus spores; about 0.01% to about 15% by weight of the composition of a pro-fragrance material selected from the group consisting of glycosides, phosphate esters, amino acid derivatives, carboxylic acid derivatives, and mixtures thereof; and a fragrance.

Description

[Technical Field]

[0001] The present invention is in the field of treatment compositions. Specifically, the present invention is directed to a treatment composition comprising a bacterial spore, a pro-perfume material, and a fragrance. The present invention also relates to a method of treating a surface with the composition. The compositions and methods of the present invention provide sustained reduction and / or prevention of malodors while simultaneously providing long-lasting fragrance release from the surface. [Background technology]

[0002] In many consumer products, it is desirable for fragrance to be released slowly over time. Although many long-lasting (long-lasting) odorants are known, many odorants are too volatile to be perceived for more than a few hours after their application. This is why there is a strong need for more efficient and effective fragrance delivery systems, especially for fabric care and home care products. To help reduce volatility, improve stability, and enable sustained release, delivery systems such as microcapsules have been developed and are already in use.

[0003] Another approach consists in using fragrance precursors or "pro-perfumes." Fragrance precursors for scenting fabrics washed in the presence of lipase-containing detergents are described in WO 95 / 04809. The fragrance precursors are cleaved by lipase to produce either a single odorous compound, an odorous alcohol, an aldehyde, or a ketone. However, these compositions may exhibit in-product stability. EP 1077251 discloses a fabric softening composition comprising a surfactant as a fabric softener active, a fragrance precursor, and an enzyme suitable for cleaving the fragrance precursor upon application in the fabric rinsing step. WO 2016142329 discloses glucoside fragrances that can be activated by glucosidase. [Prior art documents] [Patent documents]

[0004] [Patent Document 1] International Publication No. 95 / 04809 [Patent Document 2] European Patent No. 1077251 [Patent Document 3] International Publication No. 2016142329 Summary of the Invention [Problem to be solved by the invention]

[0005] There remains a need to find alternatives to pro-perfume activation to provide long-lasting fragrance on surfaces.

[0006] There is a need for methods and compositions that provide long-lasting malodor elimination and / or prevention, as well as long-lasting perfume delivery. [Means for solving the problem]

[0007] According to a first aspect of the present invention, there is provided a surface treatment composition comprising Bacillus spores, a pro-perfume material, and a fragrance. The pro-perfume material is selected from the group consisting of glycosides, phosphate esters, amino acid derivatives, carboxylic acid derivatives, and mixtures thereof. Preferably, the pro-perfume material comprises a glycoside. Preferably, the pro-perfume material comprises an alkyl monoglucoside and / or alkyl polyglucoside.

[0008] The surface treatment composition of the present invention comprises: a) about 1 × 10 of the composition 2 ~Approx. 1×10 9 CFU / g, preferably 1 x 10 3 ~Approx. 1×10 7 CFU / g, more preferably 1 x 10 4 ~Approx. 1×10 7 CFU / g of Bacillus spores and b) from about 0.01% to about 15%, preferably from about 0.01% to about 10%, by weight of the composition, of a pro-perfume material selected from the group consisting of glycosides, phosphate esters, amino acid derivatives, carboxylic acid derivatives, and mixtures thereof; c) The fragrance is preferably contained in an amount of about 0.001% to about 10%, more preferably about 0.001% to about 5%, based on the weight of the fragrance composition.

[0009] According to a second aspect of the present invention, there is provided a method of providing lasting freshness to a surface, comprising treating the surface with a composition of the present invention. Preferably, the surface is a fabric or hard surface. Preferably, the method is a cleaning process. Preferably, the method is a laundry process.

[0010] The compositions and methods of the present invention provide not only freshness during use, but also lasting freshness to surfaces, preferably fabrics or hard surfaces.

[0011] Finally, there is provided a use of the composition of the present invention to provide sustained freshness on a surface, preferably on fabrics, wherein the spores are stable in the composition and do not germinate until the treated surface is exposed to the appropriate environment (e.g., the presence of nutrients, a specific temperature, and a specific humidity).

[0012] The elements of the composition of the invention described in relation to the first aspect of the invention apply mutatis mutandis to the other aspects of the invention. DETAILED DESCRIPTION OF THE INVENTION

[0013] The present invention encompasses compositions comprising Bacillus spores, pro-perfume materials, and fragrance. The present invention also encompasses methods of providing sustained freshness to a surface using the compositions of the present invention. The present invention also encompasses the use of the methods and compositions of the present invention to provide deposition of bacterial spores and pro-perfume materials on a surface, preferably a fabric or hard surface, followed by sustained malodor removal and / or malodor prevention and fragrance release from the surface. By "sustained freshness" is meant that malodor removal and / or prevention and fragrance release occur for at least 24 hours, preferably at least 48 hours, after the surface has been treated.

[0014] As used herein, the articles "a" and "an," when used in a claim, are understood to mean one or more of what is claimed or described. As used herein, the terms "include," "includes," and "including" are meant to be open-ended. The compositions of the present disclosure may comprise, consist essentially of, or consist of the components of the present disclosure.

[0015] All percentages, ratios, and proportions used herein are by weight of the composition unless otherwise specified. All average values ​​are calculated "by weight" of the composition unless otherwise expressly indicated. All ratios are calculated as weight / weight levels unless otherwise specified.

[0016] Unless otherwise specified, all measurements are performed at 25°C.

[0017] Unless otherwise noted, all ingredient or composition concentrations are in terms of the active portion of that ingredient or composition and are exclusive of impurities, e.g., residual solvents or by-products, that may be present in commercial sources of such ingredient or composition.

[0018] composition The present disclosure relates to a composition for treating surfaces. The composition is suitable for use on hard and soft surfaces. Preferably, the composition of the present invention also comprises a surfactant system. The composition may be a cleaning composition. It may be a hard surface cleaning or laundry cleaning composition. In the case of hard surface cleaning, it is preferably an aqueous composition, which may be acidic or alkaline, and it may be in the form of a concentrated or ready-to-use composition. Alternatively, the hard surface cleaning composition may be in the form of beads. In the case of hard surface cleaning beads, the composition may comprise a plurality of particles, which may be: 1. A polyalkylene glycol having a weight average molecular weight of 2,000 to 40,000, which accounts for 20% to 70% of the total weight of the particles; 2. 10% to 70% of a foaming system based on the total weight of the particles; 3. Contains 0.1% to 50% of a fragrance based on the total weight of the particles.

[0019] The composition may be a laundry additive such as beads or dryer sheets. The composition may be a fabric enhancer composition.

[0020] "Hard surface cleaning composition" as used herein refers to a liquid base composition for cleaning hard surfaces, particularly those found in homes. Surfaces to be cleaned include kitchen and bathroom surfaces, such as floors, walls, tiles, windows, cabinets, sinks, showers, plasticized shower curtains, washbasins, toilets, fixtures, and accessories, made of different materials such as ceramic, vinyl, wax-free vinyl, linoleum, melamine, glass, steel, kitchen countertops, any plastic, plasticized wood, metal, or any painted, varnished, or sealed surface. Household hard surfaces also include household appliances, such as, but not limited to, refrigerators, freezers, washing machines, automatic dryers, ovens, microwave ovens, dishwashers, and the like. Such hard surfaces can be found in private homes as well as in commercial, corporate, and industrial environments. Preferably, the hard surface cleaning composition is an aqueous composition.

[0021] Soft surface treatment compositions may include, but are not limited to, laundry cleaning compositions, fabric freshening compositions, laundry prewash detergents, laundry pre-treatments, laundry additives, spray products, dry cleaning agents or compositions, laundry rinse additives, wash additives, post-rinse fabric treatments, ironing aids, unit dose formulations, delayed delivery formulations, detergents contained on or in porous substrates or nonwoven sheets, and other suitable forms that may be apparent to those skilled in the art in view of the teachings herein. Such compositions may be used as laundry pre-treatments, laundry post-treatments, or may be added during the wash and / or rinse cycles of the laundry process.

[0022] The composition can be in any suitable form. The product may be in the form of a liquid composition, a granular composition, a single-compartment pouch, a multi-compartment pouch, a sheet, a lozenge or bead, a fibrous article, a tablet, a bar, a flake, or a mixture thereof. The product can be selected from a liquid, a solid, or a combination thereof.

[0023] The composition may be in liquid form. The composition may contain about 30% to about 90%, or about 50% to about 80% water by weight of the composition. The pH of the composition may be optimized to promote bacterial spore stability.

[0024] The composition may be a cleaning or additive composition and may be in the form of a unitized dose article, such as a tablet, pouch, sheet, or fibrous article. Such pouches typically include a water-soluble film, e.g., a polyvinyl alcohol water-soluble film, that at least partially encapsulates the composition. Suitable films are available from MonoSol, LLC (Indiana, USA). The composition may be enclosed in a single-compartment pouch or a multi-compartment pouch. A multi-compartment pouch may have at least two, at least three, or at least four compartments. A multi-compartment pouch may include compartments arranged side-by-side and / or stacked. The composition contained in the pouch or its compartments may be liquid, solid (e.g., powder), or a combination thereof. The pouch composition may have a relatively low amount of water, e.g., less than about 20%, or less than about 15%, or less than about 12%, or less than about 10%, or less than about 8% by weight of the detergent composition.

[0025] The composition may be in the form of a tablet or beads. The tablet may contain polyethylene glycol as a carrier. The polyethylene glycol may have a weight-average molecular weight of about 2,000 to about 20,000 daltons, preferably about 5,000 to about 15,000 daltons, and even more preferably about 6,000 to about 12,000 daltons.

[0026] The composition may contain a non-aqueous solvent that can act as a carrier and / or promote stability. Non-aqueous solvents may include organic solvents such as methanol, ethanol, propanol, isopropanol, 1,3-propanediol, 1,2-propanediol, ethylene glycol, glycerin, glycol ethers, hydrocarbons, or mixtures thereof. Other non-aqueous solvents may include lipophilic fluids such as siloxanes or other silicones, hydrocarbons, perfluorinated amines, perfluorinated and hydrofluoroether solvents, or mixtures thereof. Amine-containing solvents such as monoethanolamine, diethanolamine, and triethanolamine may be suitable.

[0027] Bacterial spores The compositions of the present invention comprise about 1×10 2 ~Approx. 1×10 9 CFU / g, preferably 1 x 10 3 ~Approx. 1×10 7 CFU / g, more preferably 1 x 10 4 ~Approx. 1×10 7 CFU / g of Bacillus spores. While bacterial spores may be present on surfaces, the methods of the present invention involve intentionally adding bacterial spores to surfaces in an amount that can provide a significant consumer benefit, particularly sustained flavor release. Preferably, the methods of the present invention include at least 1 x 10 2 CFU / g of surface, preferably 1 x 10 7 It requires intentional addition of less than 1×10 CFU / g of surface. Preferably, when the surface is a fabric and the fabric is treated in a laundry process, the level of bacterial spores is less than about 1×10 2 ~1×10 4 CFU / g of surface. Methods involving direct application, such as spraying, can result in bacterial spore levels of approximately 1 x 10 4 ~1×10 6 CFU / g surface. By "intentional addition of bacterial spores" herein is meant that the spores are added in addition to any microorganisms that may be present on the fabric.

[0028] The compositions of the present invention can be in the form of fabric treatment compositions and may be added to the wash, rinse, or dry cycle; preferably, the compositions are added to the wash or rinse cycle. The spores are not inactivated by heat at temperatures found in washing machines or dryers. The spores are fabric-persistent and provide fragrance release benefits.

[0029] The bacterial spores of the methods and compositions of the present invention can germinate on surfaces. The spores can be activated by heat, such as heat generated during fabric use or heat provided in a washing machine. The spores can germinate when the fabric is stored and / or used. The enzymes secreted by the bacteria trigger the release of perfume molecules from the pro-perfume.

[0030] The fabrics can be treated in a wet laundering process or can be treated wet after washing, for example in a dryer, or sprayed. Alternatively, the fabrics can be treated with the composition in the form of a spray to refresh the fabrics.

[0031] Microbial spores for use herein are i) capable of surviving temperatures encountered in the laundry process, ii) fabric-persistent, and iii) capable of secreting enzymes and releasing perfume from the pro-perfume material. The spores have the ability to germinate and form cells during use of the surface. The spores can be provided in liquid or solid form. Preferably, the spores are in solid form.

[0032] Some Gram-positive bacteria have a two-stage life cycle. During this life cycle, bacteria growing under certain conditions, such as in response to nutrient deficiency, can execute an elaborate developmental program leading to spore or endospore formation. Bacterial spores are protected by a coat composed of approximately 60 different proteins assembled into a biochemically complex structure with interesting morphological and mechanical properties. The protein coat is considered a static structure that provides rigidity and primarily acts as a sieve to filter out large, exogenous, toxic molecules, such as lytic enzymes. Spores are highly resistant to extreme environmental conditions and therefore play an important role in the long-term survival of a species. Spores can also remain metabolically dormant for many years. Methods for obtaining bacterial spores from vegetative cells are well known in the art. In some instances, vegetative bacterial cells are grown in liquid culture. From late logarithmic or early stationary phase, bacteria can initiate spore formation. Once the bacteria have completed sporulation, the spores can be harvested from the culture medium, for example, by centrifugation. Various methods can be used to kill or remove any remaining vegetative cells. Various methods can be used to purify spores from cellular debris and / or other materials or substances. Bacterial spores can be differentiated from vegetative cells using various techniques, such as phase contrast microscopy, automated scanning microscopy, high-resolution atomic force microscopy, or thermostability methods. Bacterial spores are generally metabolically inactive or dormant, environmentally resistant structures, making them easily selected for use in commercial microbial products. Despite their hardiness and extremely long lifespan, spores can rapidly respond to the presence of specific small molecules known as germination, which signals favorable conditions for interrupting dormancy by germination, the initial step in the process of completing their life cycle by reverting to vegetative bacteria. For example, commercial microbial products can be designed so that spores are dispersed into an environment where they encounter germs present in the environment, germinate within vegetative cells, and perform their intended function. A variety of different bacteria can form spores. Bacteria from any of these groups can be used in the compositions, methods, and kits disclosed herein.For example, the following genera: Acetonema, Alcalibacillus, Ammoniphilus, Ampibacillus, Anaerobacter, Anaerospora, Aneuribacillus, Anoxybacillus, Bacillus, Brevibacillus, Cardanaerobacter, Caloramater, Caminicella, Serrasibacillus, Clostridium, Clostridium disalibacter, Cornella, Dendrosporobacter, Desulfotomaculum, Desulfosporomusa, Desulfosporosinus S., Desulfovirgra, Desulfnispora, Desulfrispora, Filifactor, Filobacillus, Gerria, Geobacillus, Geosporobacter, Gracilibacillus, Halonatronum, Heliobacterium, Heliophyllum, Raceella, Lentinibacillus, Raisinibacillus, Mahela, Metabacterium, Moorella, Natroniella, Oceanobacillus, Olenia, Ornithinebacillus, Oxalophagus, Oxobacillus Tar, Paenibacillus, Paraliobacillus, Perospora, Perotomaculum, Piscibacillus, Planiphyllum, Pontibacillus, Propionispora, Salinibacillus, Sarsuginibacillus, Seinonella, Simazuela, Sporacetigenium, Sporoanaerobacter, Sporobacter, Sporobacterium, Sporohalobacter, Sporolactobacillus, Sporomusa, Sporosalcia, Sporotalea, Sporotomaculum, Si Some bacteria, including Enterobacter, Syntrophospora, Tenuibacillus, Tepidibacter, Teribacillus, Thalassobacillus, Thermoacetogenium, Thermoactinomyces, Thermoalkalibacillus, Thermoanaerobacter, Thermoanaeromonas, Thermobacillus, Thermoflavimicrobium, Thermovenablum, Tuberibacillus, Bulgibacillus, and / or Vulcanobacillus, can form spores.

[0033] Preferably, the bacterium capable of forming spores is a bacterium of the Bacillaceae family, for example, Aeribacillus, Allibacillus, Alkalibacillus, Alkalicoccus, Alkalihalobacillus, Alkalicactibacillus, Allobacillus, Arteribacillus, Arteribacter, Ampibacillus, Anaerobacillus, Anoxybacillus, Aquibacillus, Aquisaribacillus, Aureibacillus, Bacillus, Caldarcalibacillus, Caldibacillus, Calditericola, Caldifontisbacillus, Cameliibacillus, Serrasibacillus, Compostibacillus, Rus, Cytobacillus, Desertibacillus, Domibacillus, Ectobacillus, Evansella, Farcibacillus, Ferdinandcohina, Fermentibacillus, Fictibacillus, Filbacillus, Geobacillus, Geomicrobium, Gottfriedia, Gracilibacillus, Hallalkalibacillus, Halobacillus, Haloractibacillus, Heindrixia, Hydrogenibacillus, Lederbergia, Lentibacillus, Richfieldia, Rottidebacillus, Margaritia, Marinococcus, Mergilibacillus, Mesobacillus, Metabacillus, Microaerobacter, Natoribacillus, Natronobacillus, Neobacillus, Niaria, Oceanobacillus, Ornithinibacillus, Parageobacillus, Paraliobacillus, Paralcalibacillus, Paucisalibacillus, Pelagirhabdos, Peribacillus, Piscibacillus, Polygonibacillus, Pontibacillus, Pradosia, Prieszia, Pseudogracilibacillus, Pueribacillus, Radiobacillus, Robertomuraya, Rosellomorea, Saccharococcus, Salibacterium, Salimicrobium, The bacteria are from species of the genus Salinibacillus, Salipaldibacillus, Salirhabdus, Salisediminibacterium, Saliteribacillus, Salsiuginibacillus, Sediminibacillus, Siminovichia, Sinibacillus, Sinobacillus, Streptohalobacillus, Sacrifiella, Swionibacillus, Tenuibacillus, Tepidibacillus, Terribacillus, Terrilacticbacillus, Texcoconibacillus, Thalassobacillus, Thalassorhabdus, Thermolongibacillus, Bardibacillus, Bardibacillus, Vulcanibacillus, and Weizmania. In various examples, the bacteria are from species of Bacillus axydicola (Bacillusacidicola, Bacillus aeolius, Bacillus aerius, Bacillus aerophilus, Bacillus albus, Bacillus altitudinis, Bacillus alveayuensis, Bacillus amyloliquefaciensex, Bacillus anthracis, Bacillus aquiflavi, Bacillus atrophaeus, Bacillus australimaris, Bacillus badius, Bacillus benzoevorans, Bacillus cabrialesii, Bacillus canaveralius, Bacillus capparidis, Bacillus carboniphilus, Bacillus cereus, Bacillus chungangensis, Bacillus coahuilensis, Bacillus cytotoxicus, Bacillus decisifrondis, Bacillus ectoiniformans, Bacillus enculensis enclensis, Bacillus fengqiuensis, Bacillus fungorum, Bacillus glycinifermentans, Bacillus gobiensisgobiensis, Bacillus halotolerans, Bacillus haynesii, Bacillus horti, Bacillus inaquosorum, Bacillus infantis, Bacillus infernus, Bacillus isabeliae, Bacillus kexueae, Bacillus licheniformis, Bacillus luti, Bacillus manusensis, Bacillus marinisedimentorum, Bacillus mesophilus mesophilus, Bacillus methanolicus, Bacillus mobilis, Bacillus mojavensis, Bacillus mycoides, Bacillus nakamurai, Bacillus ndiopicus, Bacillus nitratireducens, Bacillus oleivorans, Bacillus pacificus, Bacillus pakistanensis, Bacillus paralicheniformis, Bacillus paramycoides paramycoides, Bacillus paranthracis, Bacillus pervagus, Bacillus piscicola, Bacillus proteolyticus, Bacillus pseudomycoidespseudomycoides, Bacillus pumilus, Bacillus safensis, Bacillus salacetis, Bacillus salinus, Bacillus salitolerans, Bacillus seohaeanensis, Bacillus shivajii, Bacillus siamensis, Bacillus smithii, Bacillus solimangrovi, Bacillus songklensis, Bacillus sonorensis sonorensis, Bacillus spizizenii, Bacillus spongiae, Bacillus stercoris, Bacillus stratosphericus, Bacillus subtilis, Bacillus swezeyi, Bacillus taeanensis, Bacillus tamaricis, Bacillus tequilensis, Bacillus thermocloacae, Bacillus thermotolerans, Bacillus thuringiensis thuringiensis, Bacillus tianshenii, Bacillus toyonensis, Bacillus tropicus, Bacillus vallismortis, Bacillus verzuensisThe strain may be a strain of Bacillus velezensis, Bacillus wiedmannii, Bacillus wudalianchiensis, Bacillus xiamenensis, Bacillus xiapuensis, Bacillus zhangzhouensis, or a combination thereof.

[0034] In some examples, the spore-forming bacterial strain may be a Bacillus strain, such as Bacillus sp. strain SD-6991, Bacillus sp. strain SD-6992, Bacillus sp. strain NRRL B-50606, Bacillus sp. strain NRRL B-50887, Bacillus pumilus strain NRRL B-50016, Bacillus amyloliquefaciens strain NRRL B-50017, Bacillus amyloliquefaciens strain PTA-7792 (formerly classified as Bacillus atrophaeus), Bacillus amyloliquefaciens strain PTA-7543 (formerly classified as Bacillus atrophaeus), Bacillus amyloliquefaciens strain NRRL B-50018, Bacillus amyloliquefaciens strain NRRL B-50019, Bacillus amyloliquefaciens strain NRRL B-50020, Bacillus amyloliquefaciens strain NRRL B-50021, Bacillus amyloliquefaciens strain NRRL B-50022, Bacillus amyloliquefaciens strain NRRL B-50023, Bacillus amyloliquefaciens strain NRRL B-50024, Bacillus amyloliquefaciens strain NRRL B-50025, Bacillus amyloliquefaciens strain NRRL B-50026, Bacillus amyloliquefaciens strain NRRL B-50027, Bacillus amyloliquefaciens strain NRRL B-50028, Bacillus amyloliquefaciens strain NRRL B-50029, Bacillus amyloliquefaciens strain NRRL B-50029, Bacillus amyloliquefaciens strain NRRL B-5 B-50018, Bacillus amyloliquefaciens strain PTA-7541, Bacillus amyloliquefaciens strain PTA-7544, Bacillus amyloliquefaciens strain PTA-7545, Bacillus amyloliquefaciens strain PTA-7546, Bacillus subtilis strain PTA-7547, Bacillus amyloliquefaciens strain PTA-7549, Bacillus amyloliquefaciens strain PTA-7793, Bacillus amyloliquefaciens strain PTA-7790, Bacillus amyloliquefaciens strain PTA-7791, Bacillus subtilis strain NRRL B-50136 (also known as DA-33R, ATCC accession number 55406), Bacillus amyloliquefaciens strain NRRL B-50141, Bacillus amyloliquefaciens strain NRRL B-50399, Bacillus licheniformis strain NRRL B-50014, Bacillus licheniformis strain NRRL B-50015, Bacillus amyloliquefaciens strain NRRL B-50607, Bacillus subtilis strain NRRL B-50147 (also known as 300R), Bacillus amyloliquefaciens strain NRRL B-50150, Bacillus amyloliquefaciens strain NRRL B-50154, Bacillus megaterium PTA-3142, Bacillus amyloliquefaciens strain ATCC accession number 55405 (also known as 300), Bacillus amyloliquefaciens strain ATCC accession number 55407 (also known as PMX), Bacillus pumilus NRRL B-50398 (ATCCBacillus cereus ATCC accession number 700385, PMX-1, and NRRL B-50255), Bacillus cereus ATCC accession number 700386, Bacillus thuringiensis ATCC accession number 700387 (all of the above strains are available from Novozymes, Inc., USA), Bacillus amyloliquefaciens FZB24 (e.g., isolates NRRL B-50304 and NRRL B-50349 TAEGRO® from Novozymes), Bacillus pumilus (e.g., isolate NRRL B-50349 from Bayer CropScience), Bacillus amyloliquefaciens TrigoCor (also known as "TrigoCor 1448"; e.g., Embrapa available from Cornell University, USA), ...6, Bacillus thuringiensis ATCC accession number 700387 (all of the above strains are available from Novozymes, Inc., USA), Bacillus amyloliquefaciens FZB24 (e.g., isolates NRRL isolates having Trigo accession number 144 / 88.4Lev, Cornell accession number Pma007BR-97, and ATCC accession number 202152), and combinations thereof.

[0035] In some examples, the spore-forming bacterial strain can be a Bacillus amyloliquefaciens strain. For example, the strain can be Bacillus amyloliquefaciens strain PTA-7543 (formerly classified as Bacillus atrophaeus) and / or Bacillus amyloliquefaciens strain NRRL B-50154, Bacillus amyloliquefaciens strain PTA-7543 (formerly classified as Bacillus atrophaeus), Bacillus amyloliquefaciens strain NRRL B-50154, or from other Bacillus amyloliquefaciens microorganisms.

[0036] In some examples, the spore-forming bacterial strain may be a Brevibacillus species, such as Brevibacillus brevis, Brevibacillus formosus, Brevibacillus laterosporus, or Brevibacillus parabrevis, or a combination thereof.

[0037] In some examples, the spore-forming bacterial strain may be a Paenibacillus species, such as Paenibacillus alvei, Paenibacillus amylolyticus, Paenibacillus azotofixans, Paenibacillus cookii, Paenibacillus macerans, Paenibacillus polymyxa, or Paenibacillus validus, or a combination thereof.

[0038] The bacterial spores may have an average particle size of about 2-50 microns, preferably about 10-45 microns. Bacillus spores are commercially available blended in an aqueous carrier, in which they are insoluble. Other commercially available bacillus spore blends include, but are not limited to, Freshen Free™ CAN (10X), available from Novozymes Biologicals, Inc.; Evogen® Renew Plus (10X), available from Genesis Biosciences, Inc.; and Evogen® GT (10X, 20X, and 110X), all available from Genesis Biosciences, Inc. In the foregoing list, the designations in parentheses (10X, 20X, and 110X) indicate the relative concentrations of bacillus spores.

[0039] The bacterial spores used in the compositions, methods, and products disclosed herein may or may not be heat activated. In some instances, the bacterial spores are heat activated. In some instances, the bacterial spores are not heat inactivated. Preferably, the spores used herein are heat activated. Heat activation may involve heating the bacterial spores from room temperature (15-25°C) to an optimum temperature of 25-120°C, preferably 40-100°C, and holding the optimum temperature for up to 2 hours, preferably 70-80°C for 30 minutes.

[0040] For the methods and compositions disclosed herein, a population of bacterial spores is generally used. In some examples, a population of bacterial spores may include bacterial spores from a single strain of bacteria. Preferably, a population of bacterial spores may include bacterial spores from two, three, four, five, or more strains of bacteria. Generally, a population of bacterial spores contains a majority of spores and a small number of vegetative cells. In some examples, a population of bacterial spores does not contain vegetative cells. In some examples, a population of bacterial spores may contain less than about 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 15%, 20%, 25%, 30%, 40%, or 50% vegetative cells, where the percentage of bacterial spores is calculated as ((number of vegetative cells / (number of spores in the population + number of vegetative cells in the population)) × 100). Generally, the populations of bacterial spores used in the disclosed methods, compositions, and products are stable (i.e., not germinating) and at least some individual spores in the population are capable of germination.

[0041] Professional Fragrance Materials The compositions of the present disclosure include pro-perfume materials, sometimes referred to as pro-perfumes or fragrance precursors. Pro-perfume materials typically include a covalent bond between a carrier and one or more perfume raw materials (PRMs). When the spores germinate, one or more PRMs are released upon exposure to enzymes secreted by the bacteria. The pro-perfume materials can provide a prolonged PRM release profile, resulting in a long-lasting freshness benefit. Furthermore, because the entire amount of PRM is not released at once or otherwise available, the olfactory impact of the PRM is mitigated. In the compositions of the present disclosure, such a release profile can reduce what might otherwise be experienced as an overpowering odor due to a relatively high level of fragrance.

[0042] The pro-perfume material of the compositions of the present invention comprises a PRM, which is capable of releasing the PRM when exposed to an enzyme released by bacteria.

[0043] The pro-fragrance material may gradually release PRM when the spores germinate and the bacteria contained in the spores secrete enzymes. Spore germination is not triggered during product storage, but only during and after use of the product. For example, favorable conditions for spore germination are found when the treated fabric is worn, particularly when the user's body is sweating.

[0044] Pro-perfume materials for use herein are selected from the group consisting of glycosides, phosphate esters, amino acid derivatives, and carboxylic acid derivatives, and mixtures thereof. Particularly preferred pro-perfumes for use in the compositions and methods of the present invention include glycoside pro-perfumes.

[0045] The compositions of the present invention comprise from about 0.01% to about 10%, preferably from about 0.05% to about 5%, by weight of the composition, of a pro-perfume.

[0046] glycoside Glycoside pro-perfume materials comprise at least one sugar moiety covalently attached to an aglycone moiety that is capable of releasing a "fragrance alcohol" upon hydrolysis.

[0047] As used herein, a "glycoside" is a molecule in which a sugar (the "glycone" portion or "glycone component" of the glycoside) is linked to a non-sugar (the "aglycone" portion or "aglycone component") via a glycosidic bond. Thus, a glycoside can consist of a sugar as the glycone component linked through its anomeric carbon atom to the hydroxy group of, for example, an alcohol (chemical structure R-OH) as the aglycone component.

[0048] The sugar moiety of the glycoside can include monosaccharides such as glucose, galactose, mannose, rhamnose, isose, ribose, arabinose, glucosamine, and galactosamine, and disaccharides such as lactose, maltose, sucrose, cellobiose, isomaltose, and epilactose.

[0049] The aglycone corresponding to the "fragrant alcohol" component in the glycoside can be any "fragrant alcohol." Although not an exhaustive list, a list of alcohols capable of imparting a pleasant odor is provided herein. "Fragrant alcohols" include anise alcohol, cinnamic alcohol, fenchylic alcohol, 9-decen-1-ol, phenethylol, citronellol, 3-methyl-5-phenyl-1-pentanol (manufactured by Firmenich SA., Geneva, Switzerland), Mayol® (4-isopropylcyclohexyl)methanol; manufactured by Firmenich SA., Geneva, Switzerland), dihydromyrcenol (2.6-dimethyl-7-octen-2-ol), geraniol (3.7-dimethyl-2.6-octadien-1-ol), (Z)-3-hexen-1-ol, 1-hexanol, 2-hexanol, 5-ethyl-2-nonanol, 2,6-nonadien-1-ol, borneol, 1-octen-3-ol, 4-cyclohexyl-2-methyl-2-butanol (manufacturer: Firmenich SA., Geneva, Switzerland), 2-methyl-4-phenyl-2-butanol, 2-methyl-1-phenyl-2-propanol, cyclomethylcitronellol, decanol, dihydroeugenol, 8-p-menthanol, 3,7-dimethyl-1-octanol, 2,6-dimethyl-2-heptanol, 1-dodecanol, eugenol, Florol® (tetrahydro-2-iso-butyl-4-methyl-4(2H)-pyranol; manufactured by Firmenich SA., Geneva, Switzerland), isoeugenol, linalool, Tarragol® (2-methoxy-4-propyl-1-cyclohexanol; manufactured by Firmenich SA., Geneva, Switzerland), α-terpineol, tetrahydromuglyulol, 3,7-dimethyl-3-octanol, Lyral® (4-(4-hydroxy-4-methylpentyl)-cyclohex-3-ene-l-carbaldehyde; manufactured by International Flavors and Fragrances., USA), Furaneol® (manufactured by Firmenich SA., Geneva, Switzerland), 5,6-dimethyl-l-methylethenylbicyclo[2.2.1]hept-5-ene-2-methanol (Arbozol), 2-phenylethanol, 1-phenylpropanol, 2-phenylpropanol, Lilyflore® ((2,5-dimethyl-2,3-dihydro-lH-inden-2-yl)methanol; manufactured by Firmenich SA., Geneva.Switzerland), 2,2-dimethyl-3-(3-methylphenyl)-propan-1-ol (Majantol), 2-pentylcyclopentanol, 7-hydroxy-3,7-dimethyloctanal (hydroxycitronellol), 1,1-dimethyl-2-phenylethanol, 4-cyclohexyl-2-methylbutan-2-ol, menthol, 2,6-dimethylheptan-2-ol, 2-tert-butylcyclohexanol, 4-tert-butylcyclohexanol, 2,6-dimethyl-3,5-octadien-2-ol (Muguol), 2-methyl-6-methylene-7-octen-2-ol (Myrcenol), 3,7,9-trimethyl-1,6-decadien-3-ol (isobutyllinalool), methyl salicylate, cis-3-hexenyl salicylate, 3,6-dimethyloctane The hydroxybenzoate may be selected from the group consisting of, but not limited to, 1,2-dimethyl-3-prop-1-en-2-ylcyclopentan-1-ol (Purinol), 2-methyl-4-phenylpentanol (Pamplefleur), 3-methyl-5-phenylpentanol, 3-methyl-5-(2,2,3-trimethyl-1-cyclopent-3-enyl)pentan-2-ol (Sandalore®), (E)-3,3-dimethyl-5-(2,2,3-trimethyl-3-cyclopenten-1-yl)-4-penten-2-ol (Polysantol®), 1-(2,2,6-trimethylcyclohexyl)hexan-3-ol (Norlimbanol™), (E)-4-methyldec-3-en-5-ol, and 4-(4-hydroxyphenyl)butan-2-one.

[0050] The glycoside pro-perfumes used in the present invention may be in either the α- or β-form, or a mixture of the α- and β-forms. That is, the bond between the aglycone and the sugar may be either an α- or β-bond. The β-form may be preferred because it is easily degraded by β-glucosidase enzymes produced by microorganisms.

[0051] Many of these glycosides are readily available commercially. They can also be easily synthesized by known methods. For example, they can be easily synthesized by reacting a sugar with the alcohol in the presence of an acid. It is possible to synthesize only the β-form using the well-known Konigs-Knorr reaction. Glycoside pro-fragrance may also be produced via biocatalysis, for example, using glucosyltransferases or whole cell systems. Non-limiting examples of specific suitable beta-glucoside pro-fragrance, their released "fragrance alcohols," and fragrances, all commercially available from Goldmann (Bielefeld, Germany), are listed in the following table:

[0052] Preferably, the composition of the present invention contains an alkyl polyglucoside. The alkyl polyglucoside can be selected from C6 to C18 alkyl polyglucosides. The alkyl polyglucoside can have a number average degree of polymerization of 0.1 to 3.0, preferably 1.0 to 2.0, and more preferably 1.2 to 1.6. The alkyl polyglucoside can include a blend of short-chain alkyl polyglucosides having alkyl chains containing 10 or fewer carbon atoms and medium- to long-chain alkyl polyglucosides having alkyl chains containing more than 10 carbon atoms to 18 carbon atoms, preferably 12 to 14 carbon atoms.

[0053] Short-chain alkyl polyglucosides have a unimodal chain length distribution of C8 to C10, medium- to long-chain alkyl polyglucosides have a unimodal chain length distribution of C10 to C18, while medium-chain alkyl polyglucosides have a unimodal chain length distribution of C12 to C14. In contrast, C8 to C18 alkyl polyglucosides typically have a unimodal distribution of C8 to C18 alkyl chains, such as C8 to C16. Thus, a combination of a short-chain alkyl polyglucoside surfactant with a medium- to long-chain or medium-chain alkyl polyglucoside has a broader chain length distribution, or even a bimodal distribution, than the unblended C8 to C18 alkyl polyglucoside. Preferably, the weight ratio of short-chain alkyl polyglucoside to long-chain alkyl polyglucoside is 1:1 to 10:1, preferably 1.5:1 to 5:1, and more preferably 2:1 to 4:1. It has been found that such a blend of short chain alkyl polyglucosides can result in faster dissolution of the composition in water.

[0054] C8-C16 alkyl polyglycosides are commercially available from several sources (e.g., Simusol® surfactants from Seppic Corporation; and Glucopon® 600 CSUP, Glucopon® 650 EC, Glucopon® 600 CSUP / MB, and Glucopon® 650 EC / MB from BASF Corporation). Glucopon® 215UP is a preferred short-chain alkyl polyglucoside. Glucopon® 600CSUP is a preferred medium- to long-chain alkyl polyglucoside.

[0055] Preferably, the compositions of the present invention comprise monoglucosides.

[0056] [Table 1]

[0057] phosphate ester The perfume phosphate esters used in the present invention include phosphates and pyrophosphates. The alkyl moieties corresponding to the perfume ingredients include alkyl, alkenyl, alkynyl, and aralkyl groups having 5 to 15 carbon atoms, which may be branched and may have functional groups such as amyl, nonyl, geranyl, neryl, linalyl, hexenyl, nonadienyl, phenethyl, and cinnamyl groups. The perfume ingredients that can be used are the same as those described above for glycosides, and some of them are also included in the definition provided herein.

[0058] Many of these phosphate ester derivatives are readily available commercially, and can be easily synthesized by known methods. For example, they can be easily synthesized from alkyl alcohols or alkyl halides and phosphorus oxychloride or diphosphate esters according to the known methods described in J. Org. Chem. 1989, 54, 1338-1342; or Methods. Enzymol., 110, 130 (1985).

[0059] Amino acid derivatives The amino acid derivatives used in the fragrance of the present invention include amino acid esters, amino acid carbamates, N-alkylamino acids, S-alkylamino acids, and S-oxide alkylamino acids. The amino acids constituting the amino acid derivatives include cysteine, alanine, glutamic acid, glutamine, glycine, and phenylalanine. The alkyl moiety corresponding to the fragrance component includes alkyl, alkenyl, alkynyl, and aralkyl groups having 5 to 15 carbon atoms, which may be branched and may have functional groups such as amyl, nonyl, geranyl, neryl, linalyl, hexenyl, nonadienyl, phenethyl, and cinnamyl groups. The same fragrance components as those described above for glycosides can be used, and some of them are also included in the definition provided herein.

[0060] One preferred example of an amino acid derivative is the amino acid carbamate formed between the alpha amino group of glutamine and Phenoxanol®, shown below: Similar amino acid carbamate derivatives can be formed using the same glutamine carbamate moiety and other "fragrant alcohols" (e.g., those listed in the glycosides section above).

[0061] [ka]

[0062] Carboxylic Acid Derivatives Carboxylic acid derivatives of perfumes include monocarboxylic acid esters and polycarboxylic acid esters. These include esters of "fragrance alcohols" (as defined in the examples in the glycoside section above) with monocarboxylic or polycarboxylic acids. Suitable esters may be selected from esters of monocarboxylic acids, including caprylic acid, pelargonic acid, capric acid, undecylic acid, lauric acid, tridecylic acid, myristic acid, pentadecylic acid, palmitic acid, margaric acid, stearic acid, nonadecylic acid, and arachidic acid. Suitable esters may be selected from esters of polycarboxylic acids, including succinic acid, tartaric acid, and citric acid. Suitable esters may be produced by well-established chemical processes, such as the reaction of a carboxylic acid with a "fragrance alcohol" using an acid catalyst, or the reaction of an acid halide of a carboxylic acid with a "fragrance alcohol." They may also be produced by transesterification of a lower alkyl ester, such as triethyl citrate, with a "fragrance alcohol" component.

[0063] The polycarboxylic acid derivatives used in the present invention are esters of a fragrance component and a polycarboxylic acid. Examples of polycarboxylic acids include succinic acid, tartaric acid, and citric acid. The polycarboxylic acid group may be substituted with an ethyl group or other groups. The fragrance component may be the same as those described above for glycosides. The polycarboxylic acid derivatives used in the present invention are readily available. They can also be easily synthesized by reacting a polycarboxylic acid with an alcohol corresponding to the fragrance component in the presence of an acid. They may also be synthesized by transesterification of a lower alkyl ester, such as triethyl citrate, with the fragrance component. If necessary, these polycarboxylic acid derivatives may be purified, for example, by distillation or column chromatography, before use.

[0064] Non-limiting examples of specific suitable carboxylic acid derivatives include Hexarose® supplied by Firmenich (Geneva, Switzerland). This is a monocarboxylic acid ester of palmitic acid (hexadecanoic acid) and geraniol. Another example is digeranyl succinate, which is a polycarboxylic acid ester of succinic acid and geraniol containing two moles of geraniol per mole of succinic acid. Other examples are the mixed esters of succinic acid with nerol and geraniol, and the mixed esters of succinic acid with geraniol and Hedione® (methyl 3-oxo-2-pentyl-1-cyclopentane acetate).

[0065] fragrance The composition of the present invention preferably contains about 0.001% to about 10%, more preferably about 0.001% to about 5%, of the fragrance based on the weight of the composition.

[0066] The perfume may comprise a perfume raw material selected from the group consisting of alcohols, ketones, aldehydes, esters, ethers, nitrile alkenes, and mixtures thereof. The perfume may comprise a perfume raw material selected from the group consisting of a perfume raw material having a boiling point (BP) below about 250° C. and a ClogP below about 3, a perfume raw material having a BP above about 250° C. and a ClogP above about 3, a perfume raw material having a BP above about 250° C. and a ClogP below about 3, a mixture having a BP below about 250° C. and a ClogP above about 3, and mixtures thereof. Perfume raw materials having a boiling point BP below about 250°C and a ClogP below about 3 are known as Quadrant I perfume raw materials, perfume raw materials having a BP above about 250°C and a ClogP above about 3 are known as Quadrant IV perfume raw materials, perfume raw materials having a BP above about 250°C and a ClogP below about 3 are known as Quadrant II perfume raw materials, and perfume raw materials having a BP below about 250°C and a ClogP above about 3 are known as Quadrant III perfume raw materials. In one embodiment, the perfume comprises a perfume raw material having a BP below about 250°C. In one embodiment, the perfume comprises a perfume raw material selected from the group consisting of Quadrant I, II, III perfume raw materials and mixtures thereof. In one embodiment, the perfume comprises a Quadrant III perfume raw material. Suitable Quadrant I, II, III, and IV perfume raw materials are disclosed in U.S. Patent No. 6,869,923 B1.

[0067] In one embodiment, the perfume comprises a Quadrant IV perfume raw material. Without being bound by theory, it is believed that such Quadrant IV perfume raw materials can improve the odor "balance" of the perfume. The perfume may comprise less than about 30%, less than about 20%, or even less than about 15% of such Quadrant IV perfume raw materials, based on the total weight of the perfume.

[0068] Fragrance raw materials and accords may be obtained from one or more of the following companies: Firmenich (Geneva, Switzerland), Givaudan (Argenteuil, France), IFF (Hazlet, NJ), Quest (Mount Olive, NJ), Bedoukian (Danbury, CT), Sigma Aldrich (St. Louis, MO), Millennium Specialty Chemicals (Olympia Fields, IL), Polarone International (Jersey City, NJ), Fragrance Resources (Keyport, NJ), and Aroma & Flavor Specialties (Danbury, CT).

[0069] Suitable cleaning ingredients include at least one of a surfactant system, an enzyme, an enzyme stabilizing system, a detergent builder, a chelating agent, a complexing agent, a clay soil removal / anti-redeposition agent, a polymeric soil release agent, a polymeric dispersing agent, a polymeric grease cleaner, a dye transfer inhibitor, a bleaching agent, a bleach activator, a bleach catalyst, a fabric conditioner, a clay, a suds booster, a defoamer, a suds suppressor, a corrosion inhibitor, a soil suspending agent, a dye, a hueing dye, a disinfectant, an anti-haze agent, an optical brightener, a fragrance, a saturated or unsaturated fatty acid, a calcium cation, a magnesium cation, a visual signal component, a structurant, a thickener, an anti-caking agent, a starch, a sand, a gelling agent, or any combination thereof.

[0070] Surfactant System: The composition may contain a surfactant system in an amount sufficient to impart the desired cleaning properties. In some embodiments, the composition comprises from about 0.1% to about 70% of a surfactant system, preferably from about 0.5% to about 60% by weight of the composition. Preferably, liquid compositions comprise from about 1% to about 30% of a surfactant system, by weight of the composition. For ready-to-use hard cleaning surface compositions, the composition may comprise from 0.01% to 5%, preferably from 0.1% to 4%, by weight of the composition. The surfactant system may comprise a detersive surfactant selected from anionic surfactants, nonionic surfactants, cationic surfactants, zwitterionic surfactants, amphoteric surfactants, ampholytic surfactants, and mixtures thereof. Those skilled in the art will appreciate that a detersive surfactant encompasses any surfactant or surfactant mixture that provides cleaning, stain removal, or laundry benefits to soiled materials. Preferably, the surfactant system, when present in the compositions of the present invention, comprises an anionic surfactant and a nonionic surfactant.

[0071] Anionic Surfactants. Non-limiting examples of suitable anionic surfactants include any conventional anionic surfactant, including linear alkylbenzenesulfonate (LAS), alpha-olefinsulfonate (AOS), alkyl sulfate (fatty alcohol sulfate) (AS), alcohol ethoxy sulfate (AEOS or AES), secondary alkanesulfonate (SAS), alpha-sulfofatty acid methyl ester, alkyl- or alkenyl succinic acid, or soap.

[0072] Nonionic surfactants. Suitable nonionic surfactants useful herein can include any conventional nonionic surfactant. These can include, for example, alkoxylated fatty alcohols and amine oxide surfactants. Other non-limiting examples of nonionic surfactants useful herein include C8-C18 Alkyl ethoxylates (NEODOL® nonionic surfactants (Shell), etc.); C6-C 12 Alkylphenol alkoxylate (the alkoxylate units can be ethyleneoxy units, propyleneoxy units, or combinations thereof); C 12 ~C 18 C6-C with alcohol and ethylene oxide / propylene oxide block polymer 12 Alkylphenol condensates (such as Pluronic® (BASF)); C 14 ~C 22 Medium-chain branched alcohol (BA); C 14 ~C 22 Medium-chain branched MEA (BAE x) , where x is 1 to 30); polyhydroxy fatty acid amides, as well as ether-endcapped poly(oxyalkylated) alcohol surfactants. Suitable nonionic detersive surfactants also include alkyl alkoxylated alcohols. Suitable nonionic surfactants also include those sold by BASF under the trade name Lutensol®.

[0073] Cationic surfactant. The surfactant system may comprise a cationic surfactant. When the composition of the present invention is a cleaning composition, the composition preferably does not comprise a cationic surfactant. When the composition is a fabric strengthening agent, the composition preferably comprises a cationic surfactant. Non-limiting examples of cationic surfactants include quaternary ammonium surfactants, which may have 26 or less carbon atoms, including alkoxylate quaternary ammonium (AQA) surfactants; dimethylhydroxyethyl quaternary ammonium; dimethylhydroxyethyl lauryl ammonium chloride; polyamine cationic surfactants; cationic ester surfactants; and amino surfactants, such as amidopropyldimethylamine (APA).

[0074] Zwitterionic surfactants. Examples of zwitterionic surfactants include secondary and tertiary amine derivatives, heterocyclic secondary and tertiary amine derivatives, or derivatives of quaternary ammonium compounds, quaternary phosphonium compounds, or tertiary sulfonium compounds. Betaines, including alkyl dimethyl betaines and cocodimethylamidopropyl betaines, C8-C 18 (For example, C 12 ~C 18 ) amine oxides, and sulfo- and hydroxybetaines such as N-alkyl-N,N-dimethylamino-1-propanesulfonates (wherein the alkyl group is C8-C 18 In particular embodiments, C 10 ~C 14 (It can be said that).

[0075] Examples of amphoteric surfactants include aliphatic derivatives of secondary or tertiary amines, and heterocyclic aliphatic derivatives of secondary and tertiary amines, in which the aliphatic group may be straight or branched, and one of the aliphatic substituents contains at least about 8 carbon atoms, typically about 8 to about 18 carbon atoms, and at least one of the aliphatic substituents contains an anionic water-solubilizing group, such as carboxy, sulfonate, or sulfate. Examples of compounds falling within this definition are sodium 3-(dodecylamino)propionate, sodium 3-(dodecylamino)propane-1-sulfonate, sodium 2-(dodecylamino)ethyl sulfate, sodium 2-(dimethylamino)octadecanoate, disodium 3-(N-carboxymethyldodecylamino)propane-1-sulfonate, disodium octadecyl-iminodiacetate, sodium 1-carboxymethyl-2-undecylimidazole, and sodium N,N-bis(2-hydroxyethyl)-2-sulfato-3-dodecoxypropylamine. Suitable amphoteric surfactants also include sarcosinates, glycinates, taurinates, and mixtures thereof.

[0076] Enzymes. Preferably, the composition comprises one or more enzymes. Preferred enzymes provide cleaning performance and / or fabric care benefits. Examples of suitable enzymes include, but are not limited to, hemicellulase, peroxidase, protease, cellulase, xylanase, lipase, phospholipase, esterase, cutinase, pectinase, mannanase, galactanase, pectate lyase, keratinase, reductase, oxidase, phenoloxidase, lipoxygenase, ligninase, pullulanase, tannase, pentosanase, malanase, β-glucanase, arabinosidase, hyaluronidase, chondroitinase, laccase, and amylase, or mixtures thereof. Preferably, when the composition of the present invention is a laundry composition, it comprises amylase and protease, and optionally lipase. Preferably, the composition of the present invention does not comprise glucanase.

[0077] Protease. Preferably, the composition comprises one or more proteases. Suitable proteases include metalloproteases and serine proteases, including, for example, neutral or alkaline microbial serine proteases such as subtilisin (EC 3.4.21.62). Suitable proteases include those of animal, plant, or microbial origin. In one aspect, such suitable proteases may be of microbial origin. Suitable proteases include chemically or genetically modified variants of the aforementioned suitable proteases. In one aspect, suitable proteases may be serine proteases, such as alkaline microbial proteases and / or trypsin-type proteases. Examples of suitable neutral or alkaline proteases include: (a) Subtilisin (EC3.4.21.62), in particular WO 2004067737, WO 2015091989, WO 2015091990, WO 2015024739, WO 2015143360, U.S. Pat. Nos. 6,312,936(B1), 5,679,630, 4,760,025, German Patent Publication No. 102006 Bacillus species B. lentus (B.) as described in International Publication Nos. 022216(A1), 102006022224(A1), WO 2015089447, 2015089441, 2016066756, 2016066757, 2016069557, 2016069563, and 2016069569. those derived from Bacillus such as B. lentus, B. alkalophilus, B. subtilis, B. amyloliquefaciens, B. pumilus, B. gibsonii, and B. akibaii; (b) Trypsin- or chymotrypsin-type proteases, such as trypsin (e.g., of porcine or bovine origin), including the Fusarium protease described in WO 89 / 06270 and the chymotrypsin protease derived from Cellulomonas described in WO 05 / 052161 and WO 05 / 052146. (c) Metalloproteases, particularly those derived from Bacillus amyloliquefaciens, as described in WO 07 / 044993(A2). those derived from Bacillus, Brevibacillus, Thermoactinomyces, Geobacillus, Paenibacillus, Lysinibacillus or Streptomyces species described in WO 2014194032, WO 2014194054 and WO 2014194117, Kribella alluminosa described in WO 2015193488, and Streptomyces and Lysobacter described in WO 2016075078; (d) Proteases having at least 90% identity to the subtilase from Bacillus sp. TY145, NCIMB 40339, described in WO 92 / 17577 (Novozymes A / S), including variants of this Bacillus sp. TY145 subtilase described in WO 2015024739 and WO 2016066757.

[0078] Suitable commercially available protease enzymes include those sold under the trade names Alcalase®, Savinase®, Primase®, Durazym®, Polarzyme®, Kannase®, Liquanase®, Liquanase Ultra®, Savinase Ultra®, Ovozyme®, Neutrase®, Everlase® and Esperase® by Novozymes A / S (Denmark); those sold under the trade names Maxatase®, Maxacal®, Maxapem®, Properase®, Purafect®, Purafect Prime®, Purafect Ox®, FN3®, FN4®, Excellase® and Purafect OXP® by DuPont; and those sold under the trade names Opticlean® and Optimase® by Solvay. those sold by Enzymes, available from Henkel / Kemira, namely BLAP (sequence shown in Figure 29 of U.S. Patent No. 5,352,604), and KAP available from Kao (Bacillus alkalophilus subtilisin with mutations A230V+S256G+S259N).

[0079] Amylase. Preferably, the composition may contain amylase. Suitable α-amylases include those of bacterial or fungal origin, including chemically or genetically modified variants. Preferred alkaline α-amylases are those derived from Bacillus species, such as Bacillus licheniformis, Bacillus amyloliquefaciens, Bacillus stearothermophilus, Bacillus subtilis, or other Bacillus species, such as Bacillus NCIB 12289, NCIB 12512, NCIB 12513, DSM 9375 (U.S. Pat. No. 7,153,818), DSM 12368, DSM Z12649, KSM AP1378 (WO 97 / 00324), KSM K36, or KSM K38 (EP 1,022,334). Preferred amylases include: (a) Variants described in WO 94 / 02597, WO 94 / 18314, WO 96 / 23874 and WO 97 / 43424, in particular variants in which one or more of the following positions have been substituted relative to the enzyme listed as SEQ ID NO:2 in WO 96 / 23874: 15, 23, 105, 106, 124, 128, 133, 154, 156, 181, 188, 190, 197, 202, 208, 209, 243, 264, 304, 305, 391, 408 and 444. (b) variants described in U.S. Pat. No. 5,856,164 and WO 99 / 23211, WO 96 / 23873, WO 00 / 60060 and WO 06 / 002643, in particular the AA560 enzyme listed as SEQ ID NO: 12 in WO 06 / 002643, at the following positions: 26, 30, 33, 82, 37, 106, 118, 128, 133, 149, 150, 160, 178, 182, 186, 193, 203, 214, 231, 256, 257, 258, 269, 270, 272, 283, 295, 296, 298, 299, 303, 304, 305, 311, 314, 315, 318, 319, 339, 345, 361, 378, 383, 419, 421, 437, 441, 444, 445, 446, 447, 450, 461, 471, 482, 484, preferably D183 * and G184 * Variants that also contain deletions of (c) SEQ ID NO: 4 in WO 06 / 002643, variants exhibiting at least 90% identity to the wild-type enzyme from Bacillus sp. SP722, in particular variants with deletions at positions 183 and 184, and variants described in WO 00 / 60060, which are incorporated herein by reference. (d) Variants exhibiting at least 95% identity with the wild-type enzyme of Bacillus sp. 707 (SEQ ID NO: 7 of U.S. Pat. No. 6,093,562), in particular those containing one or more of the following mutations: M202, M208, S255, R172, and / or M261. Preferably, the amylase contains one or more of the following mutations: M202L, M202V, M202S, M202T, M202I, M202Q, M202W, S255N, and / or R172Q. Particularly preferred are those containing the M202L or M202T mutations. (e) a variant described in WO 09 / 149130, preferably one showing at least 90% identity to SEQ ID NO: 1 or SEQ ID NO: 2 in WO 09 / 149130, the wild-type enzyme derived from Geobacillus Stearophermophilus or a truncated version thereof. (f) Variants exhibiting at least 89% identity to SEQ ID NO: 1 in WO2016091688, in particular those containing a deletion at positions H183+G184 and further containing one or more mutations at positions 405, 421, 422, and / or 428. (g) A variant showing at least 60% amino acid sequence identity with "PcuAmyl α-amylase" derived from Paenibacillus curdlanolyticus YK9 (SEQ ID NO: 3 in WO 2014099523). (h) A variant showing at least 60% amino acid sequence identity with "CspAmy2 amylase" from Cytophaga species (SEQ ID NO: 1 in WO 2014164777). (i) A variant showing at least 85% identity with AmyE from Bacillus subtilis (SEQ ID NO: 1 of WO 2009149271). (j) A variant exhibiting at least 90% identity with the wild-type amylase derived from Bacillus sp. KSM-K38 under accession number AB051102.

[0080] Suitable commercially available α-amylases include DURAMYL®, LIQUEZYME®, TERMAMYL®, TERMAMYL ULTRA®, NATALASE®, SUPRAMYL®, STAINZYME®, STAINZYME PLUS®, FUNGAMYL®, and BAN® (Novozymes A / S, Bagsvaerd, Denmark), KEMZYM® AT 9000 (Biozym Biotech Trading GmbH, Wehlistrasse 27b A-1200 Wien, Austria), RAPIDASE®, PURASTAR®, ENZYSIZE®, OPTISIZE HT PLUS®, POWERASE®, and PURASTAR OXAM® (Genencor International Inc., Palo Alto, CA). Alto, California), and KAM® (Kao, 14-10 Nihonbashi Kayabacho, 1-chome, Chuo-ku, Tokyo 103-8210, Japan). In one aspect, suitable amylases include NATALASE®, STAINZYME®, and STAINZYME PLUS®, and mixtures thereof.

[0081] Lipase. Preferably, the composition comprises one or more lipases, including "first cycle lipases," such as those described in U.S. Patent No. 6,939,702 (B1) and U.S. Patent Application Publication No. 2009 / 0217464. Preferred lipases are first wash lipases. The composition may comprise a first wash lipase.

[0082] Enzyme Stabilizing System. The composition may optionally comprise from about 0.001% to about 10% by weight of the composition of an enzyme stabilizing system. The enzyme stabilizing system may be any stabilizing system compatible with detersive enzymes. In the case of aqueous detergent compositions containing proteases, reversible protease inhibitors such as boron compounds, including borate, 4-formylphenylboronic acid, phenylboronic acid, and derivatives thereof, or compounds such as calcium formate, sodium formate, and 1,2-propanediol may be added to further improve stability.

[0083] Builder. The composition may optionally include a builder or builder system. Built cleaning compositions typically include at least about 1% by weight of builder, based on the total weight of the composition. Liquid cleaning compositions may include up to about 10%, and in some instances up to 8%, of builder, based on the total weight of the composition. Granular cleaning compositions may include up to about 30%, and in some instances up to 5%, of builder, based on the weight of the composition.

[0084] Builders selected from aluminosilicates (e.g., zeolite builders such as zeolite A, zeolite P, and zeolite MAP) and silicates help control mineral hardness, especially calcium and / or magnesium, in wash water or remove particulate soils from surfaces. Suitable builders can be selected from the group consisting of phosphates such as polyphosphates (e.g., sodium tripolyphosphate), especially its sodium salt; carbonates, bicarbonates, sesquicarbonates, and carbonate minerals other than sodium carbonate or sesquicarbonates; organic mono-, di-, tri-, and tetracarboxylates, especially water-soluble non-surfactant carboxylates in the form of acid, sodium, potassium, or alkanolammonium salts, and oligomeric or water-soluble low-molecular-weight polymeric carboxylates, including aliphatic and aromatic species, and phytic acid. These may be complemented, for example, by borates for pH buffering purposes, or by sulfates, especially sodium sulfate, and any other fillers or carriers that may be important in engineering stable surfactant- and / or builder-containing cleaning compositions. Additional suitable builders may be selected from citric acid, lactic acid, fatty acids, polycarboxylate builders, such as copolymers of acrylic acid, copolymers of acrylic acid and maleic acid, and copolymers of acrylic acid and / or maleic acid and other suitable ethylenic monomers with various types of additional functional groups. Also suitable for use as builders herein are those having a chain structure and in the following general anhydride form: x(MO)ySiO 2· A synthesized crystalline ion exchange material or a hydrate thereof having a composition represented by zM'O, where M is Na and / or K, M' is Ca and / or Mg, y / x is 0.5 to 2.0, and z / x is 0.005 to 1.0.

[0085] Alternatively, the composition may be substantially free of builders.

[0086] Chelating Agents. The compositions may also include one or more metal ion chelating agents. Suitable molecules include copper, iron, and / or manganese chelating agents and mixtures thereof. Such chelating agents may be selected from the group consisting of phosphonates, aminocarboxylates, aminophosphonates, succinates, polyfunctionally substituted aromatic chelating agents, 2-pyridinol-N-oxide compounds, hydroxamic acids, carboxymethyl inulin, and mixtures thereof. The chelating agents may be present in acid form or in salt form, including alkali metal salts, ammonium salts, and substituted ammonium salts thereof, and mixtures thereof.

[0087] Additional Amines: Various additional amines may be used in the compositions to enhance the removal of grease and particles from soiled materials. The compositions may contain from about 0.1% to about 10%, in some instances from about 0.1% to about 4%, and in other instances from about 0.1% to about 2%, of an additional amine, by weight of the cleaning composition. Non-limiting examples of additional amines may include, but are not limited to, polyamines, oligoamines, triamines, diamines, pentamines, tetraamines, or combinations thereof. Specific examples of suitable additional amines include tetraethylenepentamine, triethylenetetraamine, diethylenetriamine, or mixtures thereof.

[0088] Dye Transfer Inhibitors. The composition may further comprise one or more dye transfer inhibitors. Suitable dye transfer inhibitors include, for example, polyvinylpyrrolidone polymers, polyamine N-oxide polymers, copolymers of N-vinylpyrrolidone and N-vinylimidazole, polyvinyloxazolidone, polyvinylimidazole, manganese phthalocyanine, peroxidase, polyvinylpyrrolidone polymers, ethylenediaminetetraacetic acid (EDTA); diethylenetriaminepentamethylenephosphonic acid (DTPMP); hydroxyethanediphosphonic acid (HEDP); ethylenediamine N,N'-disuccinic acid (EDDS); methylglycine diacetic acid (MGDA); diethylenetriaminepentaacetic acid (DTPA); propylenediaminetetraacetic acid (PDT A); 2-hydroxypyridine-N-oxide (HPNO); or methylglycine diacetate (MGDA); glutamic acid N,N-diacetate (N,N-dicarboxymethylglutamic acid tetrasodium salt (GLDA); nitrilotriacetic acid (NTA); 4,5-dihydroxy-m-benzenedisulfonic acid; citric acid and any salt thereof; N-hydroxyethylethylenediaminetriacetic acid (HEDTA), triethylenetetraaminehexaacetic acid (TTHA), N-hydroxyethyliminodiacetic acid (HEIDA), dihydroxyethylglycine (DHEG), ethylenediaminetetrapropionic acid (EDTP), and derivatives thereof, or combinations thereof.

[0089] Bleaching Compounds, Bleaching Agents, Bleach Activators, and Bleach Catalysts. The compositions described herein may contain bleaching agents, bleach activators, and / or bleach catalysts. The bleaching components may be present in concentrations of about 1% to about 30% by weight, and in some examples, about 5% to about 20% by weight, based on the total weight of the composition. When present, the amount of bleach activator may be about 0.1% to about 60% by weight, and in some examples, about 0.5% to about 40% by weight of the composition. When the composition is a powdered laundry composition, the composition preferably contains a percarbonate bleach and a bleach activator, preferably TAED. When the composition is a liquid laundry composition, the liquid composition is preferably substantially free of bleaching compounds.

[0090] Examples of bleaching agents include oxygen bleaches, perborate bleaches, percarboxylic acid bleaches and salts thereof, peroxygen bleaches, persulfate bleaches, percarbonate bleaches, and mixtures thereof.

[0091] In some examples, the composition may also include a transition metal bleach catalyst.

[0092] Bleaching agents other than oxygen bleaches are also known in the art and can be used in the compositions, including, for example, photoactivated bleaches or preformed organic peracids such as peroxycarboxylic acids or salts thereof, or peroxysulfonic acids or salts thereof.

[0093] Brightening Agents: Optical brighteners or other brightening or whitening agents may be incorporated at a level of from about 0.01% to about 1.2% by weight of the composition.

[0094] Commercially available whitening agents that may be used herein can be divided into subgroups that include, but are not necessarily limited to, derivatives of stilbenes, pyrazolines, coumarins, benzoxazoles, carboxylic acids, methine cyanines, dibenzothiphene-5,5-dioxide, azoles, 5- and 6-membered heterocycles, and various other agents.

[0095] In some examples, the fluorescent whitening agent is disodium 4,4'-bis{[4-anilino-6-morpholino-s-triazin-2-yl]-amino}-2,2'-stilbenedisulfonate (whitening agent 15, commercially available under the trade name Tinopal AMS-GX by Ciba-Geigy Corporation), disodium 4,4'-bis{[4-anilino-6-(N-2-bis-hydroxyethyl)-s-triazin-2-yl]-amino}-2,2'-stilbenedisulfonate (commercially available under the trade name Tinopal UNPA-GX by Ciba-Geigy Corporation), disodium 4,4'-bis{[4-anilino-6-(N-2-hydroxyethyl-N-methylamino)-s-triazin-2-yl]-amino}-2,2'-stilbenedisulfonate (commercially available under the trade name Tinopal 5BM-GX by Ciba-Geigy Corporation), or disodium 4,4'-bis{[4-anilino-6-(N-2-hydroxyethyl-N-methylamino)-s-triazin-2-yl]-amino}-2,2'-stilbenedisulfonate (commercially available under the trade name Tinopal 5BM-GX by Ciba-Geigy Corporation). More preferably, the fluorescent whitening agent is disodium 4,4'-bis{[4-anilino-6-morpholino-s-triazin-2-yl]-amino}-2,2'-stilbenedisulfonate.

[0096] The whitening agent may be added in particulate form or as a premix with a suitable solvent, such as a non-ionic surfactant, monoethanolamine, propanediol.

[0097] Fabric Hueing Agents. The composition may include a fabric hueing agent (sometimes referred to as a tinting agent, bluing agent, or whitening agent). Typically, the hueing agent imparts a blue or blue-purple hue to the fabric. Hueing agents can be used either alone or in combination to create a particular hue and / or tint different types of fabric. This can be achieved, for example, by mixing a red and a green-blue dye to produce a blue or purple hue. The hueing agent may be selected from any known chemical class of dyes, including, but not limited to, acridines, anthraquinones (including polycyclic quinones), azines, azos including premetallized azos (e.g., monoazos, diazos, trisazos, tetrakisazos, polyazos), benzodifurans and benzodifuranones, carotenoids, coumarins, cyanines, diazahemicyanines, diphenylmethanes, formasans, hemicyanines, indigoids, methanes, naphthalimides, naphthoquinones, nitro and nitroso, oxazines, phthalocyanines, pyrazoles, stilbenes, styryls, triarylmethanes, triphenylmethanes, xanthenes, and mixtures thereof.

[0098] Encapsulation. The composition may include an encapsulant. The encapsulant may include a core and a shell having an inner surface and an outer surface, the shell encapsulating the core.

[0099] Other Ingredients. The composition may further comprise a silicate. Suitable silicates may include, for example, sodium silicate, sodium disilicate, sodium metasilicate, crystalline phyllosilicate, or combinations thereof. In some embodiments, the silicate may be present at a concentration of about 1% to about 20% by weight, based on the total weight of the composition.

[0100] The compositions may further include conventional detergent ingredients such as suds boosters, suds suppressors, corrosion inhibitors, soil suspending agents, soil anti-redeposition agents, dyes, disinfectants, anti-tarnish agents, and / or optical brighteners.

[0101] The composition optionally comprises a saturated or unsaturated fatty acid, preferably a saturated or unsaturated C 12 ~C 24 The composition may further include fatty acids; deposition aids such as polysaccharides, cellulose polymers, polydiallyldimethylammonium halide (DADMAC) and random or block copolymers of DADMAC with vinylpyrrolidone, acrylamide, imidazole, imidazolinium halide, and mixtures thereof, cationic guar gum, cationic cellulose, cationic starch, cationic polyacrylamide, or combinations thereof. When present, the fatty acids and / or deposition aids may each be present in an amount of 0.1% to 10% by weight, based on the total weight of the composition.

[0102] The composition may optionally include silicone or fatty acid based suds suppressors; hueing dyes, calcium and magnesium cations, visual signaling components, anti-foaming agents (0.001% to about 4.0% by weight, based on the total weight of the composition), and / or structurants / thickeners (0.01% to 5% by weight, based on the total weight of the composition) selected from the group consisting of di- and triglycerides, ethylene glycol distearate, microcrystalline cellulose, microfiber cellulose, biopolymers, xanthan gum, gellan gum, and mixtures thereof.

[0103] additive composition The additive compositions of the present disclosure may include additional adjunct ingredients. Such adjuncts may provide additional processing benefits to the target fabric and / or they may act as stabilizing or processing aids to the composition. Suitable adjuncts may include chelating agents, chlorine scavengers, malodor-reducing materials, organic solvents, or mixtures thereof.

[0104] Fabric strengthener The composition of the present invention may be in the form of a fabric enhancer.The fabric enhancer for use herein comprises a fabric softening active.Suitable fabric softening actives include, but are not limited to, materials selected from the group consisting of quaternary ammonium compounds, amines, fatty esters, sucrose esters, silicones, dispersible polyolefins, clays, polysaccharides, fatty oils, polymer latexes, and mixtures thereof.Preferably, the fabric softening active is a quaternary ammonium compound, more preferably an ester quaternary ammonium compound, and even more preferably a diester quaternary ammonium compound.

[0105] Typical minimum levels of incorporation of fabric softening active into fabric strengthening agents are at least about 1%, alternatively at least about 2%, alternatively at least about 3%, alternatively at least about 5%, alternatively at least about 10%, alternatively at least about 12% by weight of the fabric strengthening agent. Fabric strengthening agents may typically comprise maximum levels of fabric softening active of less than about 90%, alternatively less than about 40%, alternatively less than about 30%, alternatively less than about 20% by weight of the fabric strengthening agent.

[0106] How to treat the surface The present disclosure relates to a method of treating a surface, which may be a hard or soft surface, preferably the surface is a soft surface, and more preferably the surface is a fabric.

[0107] For example, the method of the present disclosure may include contacting a fabric with a product according to the present disclosure. The contacting may occur, in whole or in part, in the presence of water. The product, or a portion thereof, may be diluted and / or dissolved in water to form a treatment solution.

[0108] The method of the present disclosure may include contacting a surface, preferably a fabric, with an aqueous treatment solution. The aqueous treatment solution may comprise about 1×10 per liter of wash solution. 2 Colony forming unit (CFU) ~ approx. 1 x 10 8 CFU, preferably about 1 × 10 per liter of irrigation fluid4 CFU ~ approx. 1 x 10 7 It may include CFU of total bacterial spores, preferably Bacillus spores.

[0109] The method for treating fabrics can be carried out in whole or in part in any suitable container, for example, in an automatic washing machine. Such a machine can be a top-loading machine or a front-loading machine. The entire process can be carried out in the washing machine. Alternatively, part of the process can be carried out in the washing machine and part of the process can be carried out in the dryer. The method of the present invention is also suitable for hand washing applications.

[0110] The treatment step may be part of the wash or rinse cycle of an automatic washing machine. The aqueous treatment solution may be an aqueous rinse solution. The product according to the present disclosure may be added to the drawer or drum of the automatic washing machine during the wash or rinse cycle.

[0111] The treatment step of the disclosed method may include contacting fabrics with an aqueous wash liquor. The step of contacting fabrics with the aqueous wash liquor may occur before contacting the fabrics with an aqueous rinse liquor. Such a step may occur during a single treatment cycle. The aqueous wash liquor may include a cleaning composition, such as a granular or liquid laundry detergent composition, dissolved or diluted in water. The detergent composition may include an anionic surfactant. The aqueous wash liquor may include from about 50 ppm to about 5000 ppm, or from about 100 ppm to about 1000 ppm, of anionic surfactant.

[0112] The bacterial spores, preferably Bacillus spores, may be added from an additive composition at a concentration of about 0.01% to about 5% by weight of the fabric. Preferably, the bacterial spores are provided as part of a bead or as part of a dryer sheet.

[0113] The treated fabric may be a synthetic fabric. Suitable synthetic fabrics include polyester, acrylic, nylon, rayon, acetate, spandex, latex, and / or Orlon fabrics. The compositions and methods of the present invention provide excellent malodor removal and / or prevention on synthetic fabrics.

[0114] The treated fabric may comprise synthetic fibers. Suitable synthetic fibers may include polyester, acrylic, nylon, rayon, acetate, spandex, latex, and / or Orlon fibers. The fibers may be elastic and / or contain elastane. The fabric may comprise a blend of synthetic and natural fibers (e.g., a polycotton blend). The fabric may comprise fibers that are relatively hydrophobic (e.g., compared to cotton fibers). [Example]

[0115] To determine the effect of Bacillus spores on the release of the fragrance alcohol thymol from its glucosides, the following experiment was performed. Knitted cotton swatches with thymol glucoside were treated with and without Bacillus spores. Thymol release was quantified using GC-MS.

[0116] Prior to testing, knitted cotton swatches (GMT desized knitted cotton, Warwick Equest Ltd.) were autoclaved and sterilized at 121°C for 20 minutes. Twelve swatches were placed in twelve 20 mL GC-MS vials, injected with 0.5 mL of thymol-bD-glucopyranoside (product code: MT06890, Carbosynth) in ethanol (27 wt%), and allowed to dry overnight in a fume hood. The swatches were then injected with 1 mL of tryptic soy broth (product code: 22092, Sigma Aldrich) in water (3 wt%). Half of the sample was dissolved in sterile water to give a 4.18 x 10 solution. 6One half was treated with 100 mL of a blended suspension of bacillus spores at 100 cfu / mL, and the other half was treated with 100 μL of sterile water. The bacillus spores used were Evozyme® P500 BS7, Genesis Biosciences, Cardiff. Swatches were incubated in sealed GC-MS vials in a 35°C oven for 72 hours and then left at room temperature for 96 hours before analysis.

[0117] The headspace above the fabric was sampled and analyzed using GC-MS. This involved incubating the sample at 65°C for 10 minutes before sampling the headspace with a DVB / CAR / PDMS-SPME fiber (divinylbenzene / carboxene / polydimethylsiloxane-solid-phase microextraction). The fiber was then desorbed at the hot inlet of an Agilent 7890B gas chromatograph. Thymol present in the headspace was separated from other molecules on a DB-5 column and identified using an Agilent 5977B mass spectrometer.

[0118] The table below shows the average thymol mass spectrometry peak abundance in the headspace for both treatments, along with the standard error.

[0119] [Table 2]

[0120] The results show that the presence of Bacillus spores increases the release of thymol from thymol glucoside by more than 7-fold compared to the control, a result that is statistically significant at the 99% confidence level.

[0121] The dimensions and values ​​disclosed herein should not be understood as being strictly limited to the exact numerical values ​​recited. Instead, unless otherwise specified, each such dimension is intended to mean both the recited value and a functionally equivalent range surrounding that value. For example, a dimension disclosed as "40 mm" is intended to mean "about 40 mm." This specification discloses the following inventions. [1] A surface treatment composition comprising: a) about 1×10 of said composition 2 ~Approx. 1×10 9 CFU / g of Bacillus spores and b) from about 0.01% to about 15%, by weight of the composition, of a pro-perfume material selected from the group consisting of glycosides, phosphate esters, amino acid derivatives, carboxylic acid derivatives, and mixtures thereof; c) a fragrance. [2] The composition according to [1], wherein the Bacillus is selected from the group consisting of Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus licheniformis, Bacillus megaterium, Bacillus pumilus, Bacillus cereus, Bacillus thuringiensis, Bacillus mycoides, Bacillus tequilensis, Bacillus vallismortis, Bacillus mojavensis, and mixtures thereof. [3] The composition according to [2], wherein the Bacillus is selected from the group consisting of Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus licheniformis, Bacillus megaterium, Bacillus pumilus, and mixtures thereof. [4] The composition according to any one of [1] to [3], wherein the pro-flavoring material comprises a glycoside. [5] The composition described in [4], wherein the glycoside comprises a β-D-glycoside. [6] [4] The composition described in [4], wherein the pro-fragrance material comprises an alkyl glucoside. [7] The composition according to any one of [1] to [6], wherein the composition is a cleaning composition containing a surfactant system. [8] 8. The composition according to [7], wherein the composition is a hard surface cleaning composition, preferably an aqueous cleaning composition in the form of a concentrated or ready-to-use composition. [9] [7] The composition according to [7], wherein the cleaning composition is a laundry cleaning composition containing an enzyme.

[10] [9] The composition of claim 9, wherein the composition comprises an adjuvant comprising one or more of a peroxy compound, a bleach activator, an anti-redeposition agent, a neutralizing agent, an optical brightener, a suds suppressor, a chelating agent, a bittering agent, a dye transfer inhibitor, a stain release agent, a water softener, an electrolyte, a pH adjuster, a graying inhibitor, an anti-wrinkle ingredient, a bleaching agent, a colorant, a fragrance, a processing aid, and mixtures thereof.

[11] The composition according to any one of [1] to [6], wherein the composition is a laundry additive in the form of beads or dryer sheets.

[12] The composition according to any one of [1] to [6], wherein the composition is a fabric enhancer containing a conditioning agent, and preferably the conditioning agent contains a quaternary ammonium compound, more preferably an ester quaternary ammonium compound, and even more preferably a diester quaternary ammonium compound.

[13] A method for providing lasting freshness to a surface, the method comprising the step of treating the surface with the composition according to any one of [1] to

[12] .

[14] Use of the composition according to any one of [1] to

[12] for providing lasting freshness to a surface.

Claims

1. A composition for application to fabrics, comprising: a) 1 x 10 of the composition ２ ~1 x 10 ９ CFU / g of Bacillus spores; b) 0.01% to 15%, by weight of the composition, of a pro-perfume material; and c) a perfume, wherein the pro-perfume material comprises a β-D-glycoside.

2. 2. The composition of claim 1, wherein the Bacillus is selected from the group consisting of Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus licheniformis, Bacillus megaterium, Bacillus pumilus, Bacillus cereus, Bacillus thuringiensis, Bacillus mycoides, Bacillus tequilensis, Bacillus vallismortis, Bacillus mojavensis, and mixtures thereof.

3. 3. The composition of claim 2, wherein the Bacillus is selected from the group consisting of Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus licheniformis, Bacillus megaterium, Bacillus pumilus, and mixtures thereof.

4. The composition of claim 1 , wherein the composition is a cleaning composition comprising a surfactant system.

5. The composition of claim 4, wherein the cleaning composition is an enzyme-containing laundry cleaning composition.

6. 6. The composition of claim 5, wherein the composition comprises adjuvants comprising one or more of peroxy compounds, bleach activators, anti-redeposition agents, neutralizing agents, optical brighteners, suds suppressors, chelating agents, bittering agents, dye transfer inhibitors, stain release agents, water softeners, electrolytes, pH adjusters, graying inhibitors, anti-wrinkle ingredients, bleaching agents, colorants, processing aids, and mixtures thereof.

7. The composition of claim 1 , wherein the composition is a laundry additive in the form of beads or dryer sheets.

8. The composition of claim 1 , wherein the composition is a fabric enhancer that includes a conditioning agent.

9. 9. A method for providing lasting freshness to a fabric surface, comprising applying to said fabric surface a composition according to any one of claims 1 to 8.

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