Cocoa and / or malt beverage products

Abstract

The present invention relates to cocoa and / or malt beverage products containing isomaltooligosaccharides and methods for making such products, which may have reduced sugar compared to conventional cocoa and / or malt beverage products while providing similar sweetness, texture, and taste to conventional products.

Description

[Technical Field]

[0001] The present invention relates to cocoa and / or malt beverage products containing isomaltooligosaccharides and methods for making such products.

[0002] [Background technology] Many food products traditionally contain a large amount of sugar, which is important for achieving the sweetness and texture that consumers expect from the product. For health reasons, it is desirable to reduce the amount of sugar in many food products and replace it with healthy ingredients such as fiber, but it is desirable to achieve this without sacrificing taste and texture in order to ensure consumer preference for the product. One solution is to use artificial sweeteners that provide sweetness without providing the energy of sugar, but artificial sweeteners do not contribute to the texture that sugar does, and many consumers want to avoid artificial ingredients such as artificial sweeteners. For example, many food products, such as cocoa and / or malt beverages, traditionally contain sucrose to achieve the desired taste and texture. For the above reasons, it is desirable to reduce the amount of sugar while maintaining the taste and texture of the original product, and preferably replace sugar with healthy ingredients.

[0003] Isomaltooligosaccharides are a class of short-chain carbohydrates, some of which are resistant to digestion in the human intestine, and may have prebiotic effects and a low glycemic index.

[0004] [Summary of the Invention] The inventors have discovered that a cocoa and / or malt beverage product in which a portion of barley-derived starch has been converted to isomaltooligosaccharides can be produced with a reduced sugar content while maintaining the same taste, sweetness, and texture as a conventional all-sugar product. Accordingly, the present invention relates to a cocoa and / or malt beverage product comprising 10-35% by weight of non-fat milk solids, 0-15% by weight of cocoa solids, 6-20% by weight of vegetable oil and / or milk fat, 0-25% by weight of sucrose, and 10-65% by weight of hydrolyzed barley solids, wherein the total amount of maltose is 1% to 8% by weight, and the total amount of isomaltose, isomaltotriose, and panose is 2% to 10% by weight.

[0005] In another aspect, the present invention relates to a liquid cocoa and / or malt beverage product comprising 3-10% by weight of non-fat milk solids, 0-5% by weight of cocoa solids, 0.3-5% by weight of vegetable oil and / or milk fat, 0-5% by weight of sucrose, and 1-6% by weight of hydrolyzed barley solids, wherein the total amount of maltose is 0.05% to 0.7% by weight, and the total amount of isomaltose, isomaltotriose, and panose is 0.1% to 1% by weight.

[0006] In a further aspect, the present invention relates to a method for producing the cocoa and / or malt beverage product of the present invention.

[0007] [Mode for Carrying Out the Invention] Transglucosidase, also known as alpha-glucosidase, refers to one or more enzymes having the activity of enzyme classification EC 3.2.1.20, which catalyzes the hydrolysis of (1->4)-linked alpha-D-glucose residues at the non-reducing end, resulting in the release of alpha-D-glucose. The transglucosidase of the present invention may have only transglucosidase activity or may further have one or more secondary activities. In particular, the transglucosidase of the present invention has the activity of enzyme classification EC 2.4.1.24, which catalyzes the transfer of alpha-D-glucosyl residues in (1->4)-alpha-D-glucan to the primary hydroxy group of free or incorporated glucose, e.g., to produce isomaltose from D-glucose and panose from maltose. In a preferred embodiment of the present invention, the transglucosidase has the activity of both enzyme classification EC 3.2.1.20 and enzyme classification EC 2.4.1.24.

[0008] Alpha-amylase is understood to mean one or more enzymes having the activity of an enzyme of the enzyme classification EC 3.2.1.1, which catalyzes the endohydrolysis of (1->4)-alpha-D-glucosidic bonds in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units. The alpha-amylase according to the invention may have only alpha-amylase activity or may additionally have one or more secondary activities.

[0009] Pullulanase is understood to mean one or more enzymes having the enzymatic activity of enzyme classification EC 3.2.1.41, which catalyzes the hydrolysis of (1->6)-alpha-D-glucosidic bonds in pullulan, amylopectin, and glycogen, and in the alpha- and beta-limit dextrins of amylopectin and glycogen. The pullulanase according to the present invention may have only pullulanase activity or may additionally have one or more secondary activities.

[0010] The EC (Enzyme Commission) number refers to the definition of enzyme activity and nomenclature established by the Nomenclature Commission of the International Union of Biochemistry and Molecular Biology, effective August 26, 2019.

[0011] Isomaltooligosaccharides are glucose oligomers with alpha-D-(1,6) linkages. In the context of the present invention, the term isomaltooligosaccharides is used to describe oligosaccharides selected from the group consisting of isomaltose, isomaltotriose, panose, and combinations thereof.

[0012] Cocoa and / or malt beverage products In one embodiment, the present invention relates to a cocoa and / or malt beverage product. By cocoa and / or malt beverage product, we mean a cocoa and / or malt beverage in liquid form that can be consumed as is, or a cocoa and / or malt beverage powder that can be used to prepare a cocoa and / or malt beverage by suspending it in an aqueous liquid, such as water or milk. The cocoa and / or malt beverage product of the present invention comprises non-fat milk solids, cocoa solids, vegetable oil and / or milk fat, and hydrolyzed barley solids.

[0013] The non-fat milk solids may be derived from any suitable dairy source, such as liquid milk, e.g., skim milk and / or whole milk, and / or milk powder, e.g., skim milk powder and / or whole milk powder. If liquid milk is used, it may be concentrated, for example, by evaporation or filtration.

[0014] Hydrolyzed barley refers to barley that has been enzymatically hydrolyzed with carbohydrate-degrading enzymes, for example, with alpha-amylase. The hydrolysis may be carried out by the use of purified enzymes and / or enzyme preparations, or may be carried out using endogenous enzymes of the barley malt, for example, as in conventional mashing processes, or may be a combination of the use of endogenous and added enzymes. The enzyme preparation used may contain, for example, alpha-amylase, cellulase, xylanase, beta-glucanase, pullulanase, proteinase and / or lipase. An example of a suitable commercially available enzyme preparation is Ondea® ProA (Novozymes A / S, Denmark).

[0015] The cocoa solids may be derived from any suitable cocoa source, for example, in the form of cocoa powder, cocoa mass, and / or cocoa butter. The vegetable oil may be any suitable vegetable oil, preferably palm oil. The milk fat may be derived from any suitable dairy source, such as liquid milk or cream, e.g., skim milk, cream, and / or whole milk, and / or milk powder, e.g., skim milk powder, cream powder, and / or whole milk powder, and / or may be in the form of butter, butter oil, and / or anhydrous milk fat. If liquid milk or cream is used, it may be concentrated, for example, by evaporation or filtration.

[0016] The cocoa and / or malt beverage powders of the present invention may contain sucrose. Due to the amount of glucose and isomaltooligosaccharides present in the product, the amount of sucrose can be reduced compared to conventional cocoa and / or malt beverage powders while retaining acceptable taste, sweetness, and texture. In a preferred embodiment, the cocoa and / or malt beverage product of the present invention is sucrose-free. In another preferred embodiment, the cocoa and / or malt beverage powder of the present invention contains 1 to 25% by weight of sucrose, more preferably 5 to 20% by weight of sucrose.

[0017] The cocoa and / or malt beverage powders of the present invention may include any other suitable ingredients known in the art, such as, for example, vitamins, minerals, buffer salts, emulsifiers, and stabilizers.

[0018] The cocoa and / or malt beverage powders of the present invention may be produced by any suitable method known in the art, for example, the ingredients may be in powder form and dry mixed, some or all of the ingredients may be mixed in an aqueous solution / suspension and then dried to a powder, or some or all of the powdered ingredients may be co-agglomerated, for example to produce a powder with improved solubility.

[0019] Cocoa and / or malt beverage powder In one embodiment, the cocoa and / or malt beverage product of the present invention is a cocoa and / or malt beverage powder comprising 10-35% by weight of non-fat milk solids, 0-15% by weight of cocoa solids, 6-20% by weight of vegetable oil and / or milk fat, 0-25% by weight of sucrose, and 10-65% by weight of hydrolyzed barley solids, with a total amount of maltose of 1% to 8% by weight and a total amount of isomaltose, isomaltotriose, and panose of 2% to 10% by weight.

[0020] In a preferred embodiment, the cocoa and / or malt beverage powder of the present invention comprises 10 to 50% by weight of hydrolyzed barley solids, more preferably 10 to 40% by weight of hydrolyzed barley solids, even more preferably 15 to 30% by weight of hydrolyzed barley solids.

[0021] The cocoa and / or malt beverage powders of the present invention may contain sucrose. Due to the amount of glucose and isomaltooligosaccharides present in the product, the amount of sucrose can be reduced compared to conventional cocoa and / or malt beverage powders while retaining acceptable taste, sweetness, and texture. In a preferred embodiment, the cocoa and / or malt beverage product of the present invention is sucrose-free. In another preferred embodiment, the cocoa and / or malt beverage powder of the present invention contains 1 to 25% by weight of sucrose, more preferably 5 to 20% by weight of sucrose.

[0022] In a preferred embodiment, the cocoa and / or malt beverage powder of the present invention comprises 3% to 7% by weight of maltose. In another preferred embodiment, the cocoa and / or malt beverage powder of the present invention comprises 2% to 8% by weight of isomaltose, isomaltotriose, and panose.

[0023] In a further preferred embodiment, the present invention relates to a cocoa and / or malt beverage powder comprising 10-35% by weight of non-fat milk solids, 0-15% by weight of cocoa solids, 6-20% by weight of vegetable oil and / or milk fat, 5-20% by weight of sucrose, and 10-40% by weight of hydrolyzed barley solids, wherein the total amount of maltose is 3% to 7% by weight, and the total amount of isomaltose, isomaltotriose, and panose is 2% to 8% by weight.

[0024] Liquid cocoa and / or malt beverage products In one embodiment, the cocoa and / or malt beverage product of the present invention is a liquid cocoa and / or malt beverage product comprising 3-10% by weight of non-fat milk solids, 0-5% by weight of cocoa solids, 0.3-5% by weight of vegetable oil and / or milk fat, 0-5% by weight of sucrose, and 1-6% by weight of hydrolyzed barley solids, with a total maltose content of 0.05% to 0.7% by weight and a total of isomaltose, isomaltotriose, and panose of 0.1% to 1% by weight.

[0025] In a preferred embodiment, the liquid cocoa and / or malt beverage product of the present invention comprises 1 to 5% by weight hydrolyzed barley solids, more preferably 1 to 4% by weight hydrolyzed barley solids, and even more preferably 1.5 to 3% by weight hydrolyzed barley solids.

[0026] The liquid cocoa and / or malt beverage products of the present invention may contain sucrose. Due to the amount of glucose and isomaltooligosaccharides present in the product, the amount of sucrose can be reduced compared to conventional liquid cocoa and / or malt beverage products while retaining acceptable taste, sweetness, and texture. In a preferred embodiment, the liquid cocoa and / or malt beverage products of the present invention are sucrose-free. In another preferred embodiment, the liquid cocoa and / or malt beverage products of the present invention contain 1-5% by weight of sucrose, more preferably 2-4% by weight of sucrose.

[0027] In a preferred embodiment, the liquid cocoa and / or malt beverage product of the present invention contains 0.1% to 0.5% by weight of maltose. In another preferred embodiment, the liquid cocoa and / or malt beverage product of the present invention contains 0.1% to 0.8% by weight of isomaltose, isomaltotriose and panose.

[0028] In a further preferred embodiment, the present invention relates to a liquid cocoa and / or malt beverage product comprising 3-10% by weight of non-fat milk solids, 0-5% by weight of cocoa solids, 0.3-5% by weight of vegetable oil and / or milk fat, 0-5% by weight of sucrose, and 1-4% by weight of hydrolyzed barley solids, wherein the total amount of maltose is 0.1% to 0.5% by weight, and the total amount of isomaltose, isomaltotriose, and panose is 0.1% to 0.8% by weight.

[0029] method In one embodiment, the present invention relates to a method for producing a cocoa and / or malt beverage product of the present invention, the method comprising the steps of: The barley used in the methods of the present invention may be in the form of, for example, barley grass and / or barley malt. The barley grass or barley malt may be treated in any suitable manner to facilitate hydrolysis; typically, the barley is milled or ground to increase surface area and facilitate access of the alpha-amylase substrate. The barley to be hydrolyzed may be combined with one or more additives, such as, for example, tapioca, cassava, corn, or rice starch; the slurry to be hydrolyzed by the alpha-amylase may contain, for example, 30-70% by weight of the additive, e.g., 30-70% by weight of tapioca starch.

[0030] The barley slurry is hydrolyzed with alpha-amylase. Hydrolysis of the barley slurry can be carried out by adding alpha-amylase to the slurry, for example, in the form of pure alpha-amylase or in the form of an enzyme preparation containing alpha-amylase. Hydrolysis of the barley slurry can also be carried out by using barley malt for the slurry. Barley malt contains endogenous alpha-amylase as well as other carbohydrate-degrading enzymes, which react with the carbohydrates of the barley malt in the slurry. The purpose of hydrolysis is to convert the starch contained in the barley into low-molecular-weight carbohydrates and sugars, such as glucose, fructose, maltose, and maltotriose.

[0031] If the alpha-amylase is added as an enzyme preparation, the enzyme preparation may contain further enzyme activities, such as cellulase, xylanase, beta-glucanase, pullulanase, proteinase and / or lipase. The pH of the barley may be adjusted prior to contact with the enzyme preparation containing alpha-amylase, e.g., the pH may be lowered, e.g., to a pH in the range of 4.8 to 5.1. pH adjustment may be carried out in any suitable manner, e.g., by the addition of a base, e.g., sodium hydroxide and / or calcium chloride.

[0032] Barley malt is barley grain that has undergone a germination step, resulting in the formation of endogenous enzymes, including alpha-amylase. These enzymes are capable of breaking down polymeric substances, such as starch, protein, and fat, into lower molecular weight substances, such as sugars, including primarily glucose, fructose, maltose, and maltotriose, amino acids, and fatty acids. When barley malt is used, it can be treated in any suitable manner to induce hydrolysis. Hydrolysis of barley malt, also known as "mashing," is a well-known process used, for example, in the extraction and production of malt extracts.

[0033] Transglucosidase is added to a slurry of barley in an aqueous liquid to produce isomaltooligosaccharides and glucose. Any suitable transglucosidase may be used, such as a transglucosidase from Aspergillus niger, such as Transglucosidase L "Amano" (Amano Enzyme Co., Ltd.). The addition may be carried out at any suitable temperature and for any suitable time, taking into account the characteristics of the transglucosidase. The transglucosidase may be added to the slurry before, during, and / or after hydrolysis of the slurry with alpha-amylase. In a preferred embodiment, the transglucosidase is added to the slurry after the initiation of hydrolysis with alpha-amylase. In a preferred embodiment, the transglucosidase is added 10 to 60 minutes after the initiation of hydrolysis with alpha-amylase.

[0034] The temperature of the slurry can be varied between the initiation of hydrolysis with alpha-amylase and the addition of transglucosidase. In a preferred embodiment, the temperature of the slurry is increased by 5-30°C after the initiation of hydrolysis with alpha-amylase and before the addition of transglucosidase.

[0035] After the alpha-amylase hydrolysis and transglucosidase reaction have proceeded to the desired extent, the enzymatic reaction may be stopped, for example, by inactivating the enzymes. In a preferred embodiment, after adding transglucosidase to inactivate the enzymes, the temperature of the slurry is increased to between 70°C and 90°C.

[0036] In one embodiment, the hydrolysis with alpha-amylase is initiated before the addition of transglucosidase, and the temperature of the slurry is between 45 and 60°C during the hydrolysis with alpha-amylase before the addition of transglucosidase.

[0037] The spent grain is removed from the slurry to produce wort. The spent grain can be removed by any suitable method, for example, by filtration or centrifugation. The spent grain can be removed before or after the addition of transglucosidase; i.e., the transglucosidase reaction can be carried out while the reacted grounds are present and / or after they have been removed. The wort can be concentrated, for example, by evaporation and / or filtration, and further dried, for example, by spray drying or roller drying. The transglucosidase can be added before or after concentration. The transglucosidase can also be added after drying, in which case the dried wort is suspended in an aqueous liquid under conditions suitable for making the transglucosidase active.

[0038] The wort may be combined with other ingredients before further processing, for example, the wort may be combined with hydrolyzed starch, such as hydrolyzed tapioca starch.

[0039] The wort is combined with non-fat milk solids, cocoa solids, vegetable oil and / or milk fat, and optionally sucrose and other ingredients to obtain a cocoa and / or malt beverage product. This can be done by any method known in the art. The ingredients can be combined in dry or liquid form, or some ingredients can be in dry form and some ingredients can be in liquid form. In one embodiment, the wort comprises 20-65% by weight of the dry solids of the final cocoa and / or malt beverage product.

[0040] If the final product is a liquid cocoa and / or malt beverage product, the moisture content of the final mixture can be adjusted in any suitable manner, for example by adding water, to obtain the final desired solids content. The product can be heat treated to improve shelf life, for example by pasteurization, UHT treatment, or sterilization, and packaged in a suitable container.

[0041] If the final product is a cocoa and / or malt beverage powder, the ingredients may, for example, be in powder form, may be dry mixed, some or all of the raw materials may be mixed in an aqueous solution / suspension and then dried to a powder, or some or all of the raw material powders may be, for example, co-agglomerated to produce a powder with improved solubility.

[0042] [Example] Example 1 A slurry of ground barley grist in water was prepared and heated to 45-60°C. An enzyme preparation (Ondea® ProA, Novozymes A / S, Denmark) containing alpha-amylase, pullulanase, cellulase, xylanase, protease, and lipase was added and allowed to react. Transglucosidase (Transglucosidase L "Amano", Amano Enzyme Co., Ltd.) was added, and the temperature was raised to 60-80°C. After the transglucosidase reaction, the temperature was raised above 80°C to inactivate the enzyme, and the slurry was filtered through a filter press to remove spent grain and produce wort. The wort was mixed with an equal weight of hydrolyzed tapioca starch. The wort was used to produce cocoa and malt beverage powders with the compositions shown in Table 1. Reference cocoa and malt beverage powders were produced with the same composition and in the same manner, except that malt extract was used instead of the wort from hydrolyzed barley and transglucosidase-treated barley. The sugar composition of the products and the reference is shown in Table 2. It can be seen that the sugar (monosaccharides and disaccharides) content is reduced by more than 14% by weight compared to the reference product. The products and the reference were reconstituted and tested by a sensory panel and found to have similar sweetness, taste, and texture.

[0043] [Table 1]

[0044] [Table 2]

Claims

1. 1. A cocoa and / or malt beverage powder comprising: 10 to 35% by weight of non-fat milk solids; 0-15% by weight of cocoa solids; 6 to 20% by weight of vegetable oil and / or milk fat; 1 to 25% by weight of sucrose; 10-50% by weight of hydrolyzed barley solids; The total amount of maltose is 3% to 7% by weight, and the total amount of isomaltose, isomaltotriose, and panose is 2% to 8% by weight, The cocoa and / or malt beverage powder is produced by a method comprising the steps of: b) hydrolyzing a slurry containing barley with an enzyme preparation containing alpha-amylase; and c) adding transglucosidase to react with the transglucosidase, wherein the temperature of the slurry is increased by 5 to 30°C after the start of step b) and before the start of step c), and the enzyme preparation in step b) further comprises cellulase, xylanase, beta-glucanase, pullulanase, proteinase and / or lipase.

2. 2. The powdered cocoa and / or malt beverage powder of claim 1, wherein the total amount of glucose, sucrose, fructose, maltose, and isomaltose is between 20% and 40% by weight.

3. 1. A liquid cocoa and / or malt beverage product comprising: 3 to 10% by weight of non-fat milk solids; 0-5% by weight of cocoa solids; 0.3 to 5% by weight of vegetable oil and / or milk fat; 1 to 5% by weight of sucrose; 1-5% by weight of hydrolyzed barley solids; The total amount of maltose is 0.1% by weight to 0.5% by weight, and the total amount of isomaltose, isomaltotriose, and panose is 0.1% by weight to 0.8% by weight, The liquid cocoa and / or malt beverage product is produced by a method comprising the steps of: b) hydrolyzing a slurry containing barley with an enzyme preparation containing alpha-amylase; and c) adding transglucosidase to react with the transglucosidase, wherein the temperature of the slurry is increased by 5 to 30°C after the start of step b) and before the start of step c), and the enzyme preparation in step b) further comprises cellulase, xylanase, beta-glucanase, pullulanase, proteinase and / or lipase.

4. 4. The liquid cocoa and / or malt beverage product of claim 3, wherein the total amount of glucose, sucrose, fructose, maltose, and isomaltose is from 1% to 3.5% by weight.

5. 1. A method of producing a cocoa and / or malt beverage powder comprising the steps of: a) suspending barley in an aqueous liquid to produce a slurry; b) hydrolyzing the slurry of step a) with an enzyme preparation comprising alpha-amylase; c) adding transglucosidase and reacting the transglucosidase; d) removing spent grain from the slurry after step b) to obtain wort; e) combining the wort obtained in step d) with non-fat milk solids, cocoa solids, sucrose, vegetable oil and / or milk fat to obtain a cocoa and / or malt beverage powder, step c) is performed during and / or after step b) and before, during and / or after step d); increasing the temperature of the slurry by 5-30°C after the start of step b) and before the start of step c); The method, wherein the enzyme preparation of step b) further comprises cellulase, xylanase, beta-glucanase, pullulanase, proteinase and / or lipase.

6. 6. The method of claim 5, wherein step c) is initiated 10 to 60 minutes after the initiation of step b).

7. 7. The method of claim 5 or 6, wherein the temperature of the slurry is increased to 70-90°C after step c) to inactivate the enzyme.

8. 8. The method of any one of claims 5 to 7, wherein step b) is initiated before step c), and the temperature of the slurry during step b) before the start of step c) is 45 to 60°C.

9. 9. The method according to any one of claims 5 to 8, wherein the wort obtained in step d) constitutes 20 to 65% by weight of the dry solids of the final cocoa and / or malt beverage powder obtained in step e).

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