topical skin preparations

Abstract

To provide a new application for fermented rice, which can be blended in external preparations for skin, and has high efficacy.SOLUTION: The present invention discloses a skin permeability promoter containing the rice fermented with lactic acid bacteria as an active ingredient and an external skin preparation for improving wrinkles containing the rice fermented with lactic acid bacteria as an active ingredient.SELECTED DRAWING: Figure 1

Description

[Technical Field]

[0001] The present invention aims to provide a new use for a highly effective fermented rice product that can be incorporated into external skin preparations. [Background technology]

[0002] Fermented rice products have been known to be used as emulsifiers for external skin preparations and the like.

[0003] [Patent Document 1] Japanese Patent Application Laid-Open No. 2002-348207 DISCLOSURE OF THE INVENTION [Problem to be solved by the invention]

[0004] Fermented rice products have been known to be used as emulsifiers, but new uses are desired. Therefore, the present inventors aimed to solve the above-mentioned problems and provide new uses for fermented rice products. [Means for solving the problem]

[0005] The present invention is a skin permeation enhancer containing a lactic acid bacteria fermentation product of rice as an active ingredient. The present invention also provides an external skin preparation for wrinkle reduction, which contains a lactic acid bacteria fermentation product of rice as an active ingredient. [Effects of the Invention]

[0006] The present invention relates to a skin permeation promoter or topical skin preparation for wrinkle improvement, which contains a lactic acid bacteria fermentation product of rice as an active ingredient. According to the present invention, it is possible to provide an agent for promoting the skin permeation of active ingredients and to provide a highly effective topical skin preparation for wrinkle improvement. [Brief explanation of the drawings]

[0007] [Figure 1] FIG. 1 is a diagram showing the results of a skin permeability evaluation test for a composition containing a lactic acid bacteria fermentation product of rice according to the present invention. [Figure 2] FIG. 1 is a diagram showing the results of a skin permeability evaluation test for a composition containing a lactic acid bacteria fermentation product of rice according to the present invention. [Figure 3] FIG. 1 is a diagram showing the results of an effectiveness evaluation test of a composition containing a lactic acid bacteria fermentation product of rice according to the present invention. DETAILED DESCRIPTION OF THE INVENTION

[0008] The present invention will be described in detail below. The rice used to obtain the lactic acid bacteria fermentation product of the present invention is not particularly limited, and any of brown rice, germinated brown rice, polished rice, etc. can be used. As for the type of rice, any type of rice can be used, such as non-glutinous rice. In addition, pigmented rice such as black rice, purple rice, red rice, and green rice can also be used.

[0009] Furthermore, the rice to be used for fermentation may be subjected to a low-protein treatment before the fermentation treatment. Methods for treating low-protein rice include washing, acid or alkali treatment, and enzyme treatment.

[0010] Washing methods include washing with water or alkaline water (preferably pH 8) during or after fermentation.

[0011] When treating with an enzyme, the treatment is carried out using the following proteases: For example, actinases such as actinase, pepsins such as pepsin, trypsins such as trypsin and chymotrypsin, papains such as papain and chymopapain, peptidases such as glycylglycine peptidase, carboxypeptidase and aminopeptidase, bromelain, etc.

[0012] In the present invention, the protein content of the processed product obtained by the above method is preferably 1.5% or less, more preferably 1.0% or less.

[0013] In the present invention, examples of microorganisms used to ferment rice include, but are not limited to, lactic acid bacteria or bifidobacteria, or a combination of these microorganisms with one or more species selected from the group consisting of koji mold, yeast, and Bacillus subtilis.

[0014] Examples of lactic acid bacteria include lactic acid bacteria of the genus Lactobacillus, such as Lactobacillus plantarum, Lactobacillus brevis, Lactobacillus casei, and Lactobacillus delbruekii; lactic acid bacteria of the genus Carnobacterium, such as Carnobacterium divergens and Carnobacterium piscicola; lactic acid bacteria of the genus Leuconostoc, such as Leuconostoc mesenteroides and Leuconostoc citreum; and Streptococcus. Lactic acid bacteria of the genus Streptococcus, such as Streptococcus faecalis and Streptococcus pyogenes; lactic acid bacteria of the genus Enterococcus, such as Enterococcus caseliflavus and Enterococcus sulfreus; lactic acid bacteria of the genus Lactococcus, such as Lactococcus plantarum and Lactococcus rafinolactis; lactic acid bacteria of the genus Veissella, such as Weissella confusa and Weissella kandleri; Atopobium minutum and Atopobium Lactic acid bacteria of the genus Atopobium, such as Atopobium parvulus; lactic acid bacteria of the genus Vagococcus, such as Vagococcus fluvialis and Vagococcus salmoninarum;Examples of such lactic acid bacteria include lactic acid bacteria of the genus Pediococcus, such as Pediococcus damnosus and Pediococcus pentosaceus, but any lactic acid bacteria can be used as long as they are classified as lactic acid bacteria.

[0015] Examples of bifidobacteria include Bifidobacterium bifidum and Bifidobacterium breve, and any of them can be used as long as they are classified as bifidobacteria.

[0016] Examples of koji mold include yellow koji molds such as Aspergillus oryzae, Aspergillus flavus, Aspergillus polyoxogenes, and Aspergillus sojae; black koji molds such as Aspergillus awamori, Aspergillus kawauchii, Aspergillus usami, and Aspergillus niger; and red koji molds such as Monascus anka and Monascus pilosus. Any koji mold can be used as long as it is classified as koji mold.

[0017] Examples of yeast include yeasts of the genus Saccharomyces such as Saccharomyces cerevisiae, Saccharomyces awamori, Saccharomyces chevalieri, Saccharomyces carlsbergensis, and Saccharomyces bayonus; yeasts of the genus Torulaspora such as Torulaspora delbruekii, Torulaspora fermentati, and Torulaspora rosei; yeasts of the genus Zygosaccharomyces rouxii and Zygosaccharomyces sojae; Yeasts of the genus Zygosaccharomyces, such as Zygosaccharomyces soybean, Zygosaccharomyces sake, Zygosaccharomyces miso, and Zygosaccharomyces lactis; yeasts of the genus Candida, such as Candida versatilis, Candida etchellsii, Candida kefyr, Candida sake, and Candida scottii; yeasts of the genus Candida, such as Aureobasidium pullulans, Aureobasidium mansonii, and Aureobasidium microstictum. Examples of yeasts that can be used include yeasts of the genus Aureobasidium, such as Aureobasidium microstictum, but any yeast that can be classified as yeast can be used.Of the above-mentioned yeasts, Saccharomyces cerevisiae is preferred from the viewpoints of safety and effectiveness, but Saccharomyces cerevisiae of any origin can be used, including those derived from plants such as sake and cherry blossoms, and those of marine origin.

[0018] Examples of Bacillus subtilis include bacteria of the genus Bacillus, such as Bacillus natto, Bacillus subtilis, and Bacillus circulans.

[0019] The fermented product according to the present invention may be gelatinized (gelatinized) by heat treatment or heat treatment under pressure. When gelatinization is carried out by heat treatment, the temperature is generally 80°C to 220°C, preferably 90°C to 160°C, and the degree of gelatinization (gelatinization) is preferably 90% or more.

[0020] When the fermented product obtained as described above is subjected to low-protein rice treatment, it is preferable that the nitrogen content be measured by, for example, the macro-Kjeldahl method or the Kjeldahl method, which is a nitrogen determination method, and that in the present invention, the content be 1.5 (g / 100) or less.

[0021] Examples of topical skin preparations (cosmetics, quasi-drugs, topical pharmaceuticals) that can be formulated with the fermented product of the present invention include basic cosmetics such as emulsions, creams, lotions, essences, packs, and facial cleansers; makeup cosmetics such as lipstick, foundation, liquid foundation, and makeup press powder; hair cosmetics such as hair shampoos, hair rinses, hair treatments, conditioners, hair growth products, hair dyes, and hair styling products; and bath additives, but are of course not limited to these.

[0022] When the fermented product of the present invention is incorporated into topical skin preparations (cosmetics, quasi-drugs, topical pharmaceuticals), ingredients commonly used in topical skin preparations, such as oily ingredients, moisturizers, thickeners, emulsifiers, anti-inflammatory agents, antiseptics / bactericides, anti-acne agents, cell activators, powder ingredients, UV absorbers, surfactants, pigments, fragrances, and other physiologically active ingredients, can be appropriately incorporated as needed. Furthermore, the antioxidant of the present invention can be incorporated into cosmetics (including quasi-drugs) in combination with other physiologically active ingredients, as long as the efficacy and characteristics of the antioxidant are not impaired.

[0023] Here, examples of oily components include plant-derived oils such as olive oil, jojoba oil, castor oil, soybean oil, rice oil, rice germ oil, coconut oil, palm oil, cacao oil, meadowfoam oil, shea butter, tea tree oil, avocado oil, macadamia nut oil, rapeseed oil, oils derived from citrus fruits (such as yuzu, lime, and mandarin orange), ginseng oil, and plant-derived squalane; animal-derived oils such as mink oil and turtle oil; waxes such as beeswax, carnauba wax, rice wax, and lanolin; liquid paraffin, petrolatum, and paraffin oil. Examples of suitable glycerols include hydrocarbons such as corn wax and squalane; fatty acids such as myristic acid, palmitic acid, stearic acid, oleic acid, isostearic acid, and cis-11-eicosenoic acid; higher alcohols such as lauryl alcohol, cetanol, and stearyl alcohol; synthetic esters and synthetic triglycerides such as isopropyl myristate, isopropyl palmitate, butyl oleate, 2-ethylhexyl glyceride, and higher fatty acid octyldodecyl (e.g., octyldodecyl stearate).

[0024] Examples of surfactants include nonionic surfactants such as polyoxyethylene alkyl ethers, polyoxyethylene fatty acid esters, polyoxyethylene sorbitan fatty acid esters, glycerin fatty acid esters, polyglycerin fatty acid esters, polyoxyethylene glycerin fatty acid esters, polyoxyethylene hydrogenated castor oil, and polyoxyethylene sorbitol fatty acid esters; fatty acid salts, alkyl sulfates, alkylbenzene sulfonates, polyoxyethylene alkyl ether sulfates, polyoxyethylene fatty amine sulfates, polyoxyethylene alkylphenyl ether sulfates, polyoxyethylene alkyl ether phosphates, α-sulfonated fatty acid alkyl ester salts, polyoxyethylene Examples of surfactants that can be used include anionic surfactants such as phenyl alkyl phenyl ether phosphates; cationic surfactants such as quaternary ammonium salts, primary to tertiary fatty amine salts, trialkylbenzylammonium salts, alkylpyridinium salts, 2-alkyl-1-alkyl-1-hydroxyethylimidazolinium salts, N,N-dialkylmorpholinium salts, and polyethylene polyamine fatty acid amide salts; and amphoteric surfactants such as N,N-dimethyl-N-alkyl-N-carboxymethylammoniobetaine, N,N,N-trialkyl-N-alkyleneammoniocarboxybetaine, and N-acylamidopropyl-N',N'-dimethyl-N'-β-hydroxypropylammoniosulfobetaine.

[0025] Examples of emulsifiers or emulsifying aids include lecithin (soybean lecithin, egg yolk lecithin, sunflower lecithin, etc.) and derivatives thereof (hydrogenated lecithin, hydroxide lecithin, etc.), saponin or derivatives thereof, casein or its salts (sodium, etc.), sugar and protein complexes, sucrose or esters thereof, lactose, soybean-derived water-soluble polysaccharides, soybean-derived protein and polysaccharide complexes, lanolin or derivatives thereof, cholesterol, stevia derivatives (enzyme-treated stevia, etc.), silicates (aluminum, magnesium, etc.), carbonates (calcium, sodium, etc.), etc.

[0026] Examples of moisturizing agents include glycerin, propylene glycol, dipropylene glycol, 1,3-butylene glycol, polyethylene glycol, sorbitol, xylitol, sodium pyrrolidone carboxylate, and the like, as well as sugars such as trehalose and raffinose, mucopolysaccharides (e.g., hyaluronic acid and its derivatives, hyaluronic acid fermentation liquid, chondroitin and its derivatives, heparin and its derivatives, etc.), elastin and its derivatives, collagen and its derivatives, collagen peptides, NMF-related substances, lactic acid, urea, higher fatty acid octyldodecyl, seaweed extract, Bletilla striata root (white extract), and various amino acids and their derivatives.

[0027] Examples of thickeners include ingredients derived from brown algae, green algae, or red algae, such as alginic acid, agar, carrageenan, and fucoidan; extracts of Bletilla striata (white algae); polysaccharides such as pectin and aloe polysaccharides; gums such as tragacanth gum, locust bean gum, xanthan gum, and guar gum; cellulose derivatives such as carboxymethyl cellulose, hydroxyethyl cellulose, and hydroxypropyl cellulose; synthetic polymers such as carboxyvinyl polymers, alkyl-modified carboxyvinyl polymers, polyvinyl alcohol, polyvinylpyrrolidone, and acrylic acid-methacrylic acid copolymers; hyaluronic acid and its derivatives; and polyglutamic acid and its derivatives.

[0028] Anti-inflammatory agents include allantoin, dipotassium glycyrrhizinate, monoammonium glycyrrhizinate, β-glycyrrhetinic acid, stearyl glycyrrhetinate, ε-aminocaproic acid, d-camphor, dl-camphor, zinc oxide, D-pantothenyl alcohol, pyridoxine hydrochloride, tranexamic acid, and riboflavin or a derivative thereof.

[0029] Examples of antiseptics and disinfectants include urea; benzoic acid or its salts, parahydroxybenzoic acid esters such as methyl parahydroxybenzoate, ethyl parahydroxybenzoate, propyl parahydroxybenzoate, and butyl parahydroxybenzoate; phenoxyethanol, dichlorophene, hexachlorophene, chlorhexidine hydrochloride, benzalkonium chloride, salicylic acid, sodium salicylate, zinc pyrithione, benzalkonium chloride, ethanol, undecylenic acid, phenols, and alkane bromide. Examples include chrysoquinolinium, resorcinol, jamal (imidazolidinyl urea), isopropyl methylphenol, triclosan, trichlorocarbanide, trichlorohydroxydiphenol ether, hinokitiol, 1,2-pentanediol, propanediol, concentrated benzalkonium chloride solution 50, essential oils such as peppermint oil and eucalyptus oil, bark distillate, radish fermented liquid, ethanol derived from plants such as sugar cane and corn, or 1,3-butylene glycol.

[0030] Examples of cell activators include D-pantothenyl alcohol, menthol, dl-menthol, and γ-oryzanol.

[0031] Anti-acne agents include sulfur, salicylic acid or its salts, photosensitizer No. 201, pyridoxine dicaprylate, and the like.

[0032] Examples of powder components include sericite, titanium oxide, talc, kaolin, bentonite, zinc oxide, magnesium carbonate, magnesium oxide, zirconium oxide, barium sulfate, silicic anhydride, mica, nylon powder, polyethylene powder, silk powder, cellulose-based powder, powder of grains (rice, wheat, corn, millet, etc.), powder of beans (soybean, adzuki bean, etc.), etc.

[0033] Examples of ultraviolet absorbers include ethyl paraaminobenzoate, ethylhexyl paradimethylaminobenzoate, amyl salicylate and its derivatives, 2-ethylhexyl paramethoxycinnamate, octyl cinnamate, oxybenzone, 2,4-dihydroxybenzophenone, 2-hydroxy-4-methoxybenzophenone-5-sulfonate, 4-tert-butyl-4-methoxybenzoylmethane, 2-(2-hydroxy-5-methylphenyl)benzotriazole, urocanic acid, and ethyl urocanate.

[0034] Examples of whitening agents include t-cycloamino acid derivatives, kojic acid and its derivatives, ascorbic acid and its derivatives, hydroquinone or its derivatives, ellagic acid and its derivatives, nicotinic acid and its derivatives, resorcinol derivatives, tranexamic acid and its derivatives, 4-methoxysalicylic acid potassium salt, magnolignan (5,5'-dipropyl-biphenyl-2,2'-diol), hydroxybenzoic acid and its derivatives, vitamin E and its derivatives, α-hydroxy acid, AMP (adenosine monophosphate, adenosine monophosphate), etc., and these may be blended alone or in combination.

[0035] Examples of the kojic acid derivatives include kojic acid esters such as kojic acid monobutyrate, kojic acid monocaprate, kojic acid monopalmitate, and kojic acid dibutyrate, kojic acid ethers, and kojic acid sugar derivatives such as kojic acid glucoside. Examples of the ascorbic acid derivatives include ascorbic acid esters such as sodium L-ascorbic acid 2-phosphate, magnesium L-ascorbic acid 2-phosphate, sodium L-ascorbic acid 2-sulfate, and magnesium L-ascorbic acid 2-sulfate. Ascorbic acid sugar derivatives such as L-ascorbic acid ester salts, L-ascorbic acid-2-glucoside, L-ascorbic acid-5-glucoside, ascorbyl tocopheryl maleate, ascorbyl tocopheryl phosphate K, myristyl 3-glyceryl ascorbate, caprylyl 2-glyceryl ascorbate, and the like; 6-acylated products of these ascorbic acid sugar derivatives (the acyl group is a hexanoyl group, an octanoyl group, a decanoyl group, etc.); L-ascorbic acid such as L-ascorbic acid tetraisopalmitate and L-ascorbic acid tetralaurate. Examples of hydroquinone derivatives include tetra-fatty acid esters, 3-O-ethyl ascorbic acid, L-ascorbic acid-2-phosphate-6-O-sodium palmitate, glyceryl ascorbic acid or its acylated derivatives, ascorbic acid glycerin derivatives such as bisglyceryl ascorbic acid, aminopropyl L-ascorbic acid phosphate, hyaluronic acid derivatives of L-ascorbic acid, 3-OD lactose-L-ascorbic acid, and isostearyl ascorbyl phosphate. Examples of hydroquinone derivatives include arbutin (hydroquinone-β-D-glucopyranoside). amide), α-arbutin (hydroquinone-α-D-glucopyranoside), etc.; tranexamic acid derivatives include tranexamic acid esters (e.g., tranexamic acid lauryl ester, tranexamic acid hexadecyl ester, tranexamic acid cetyl ester or a salt thereof), tranexamic acid amides (e.g., tranexamic acid methylamide), etc.; resorcinol derivatives include, for example, 4-n-butylresorcinol, 4-isoamylresorcinol, etc.; 2,5-dihydroxybenzoic acid derivatives include, for example, 2,Examples of nicotinic acid derivatives include nicotinamide and benzyl nicotinate, and examples of α-hydroxy acids include lactic acid, malic acid, succinic acid, citric acid, and α-hydroxyoctanoic acid.

[0036] Examples of physiologically active ingredients include placenta extract, Morus alba extract, Saxifraga extract, Perilla extract, rice bran extract or hydrolysate thereof, white mustard extract or hydrolysate thereof, fermented white mustard, peony extract or hydrolysate thereof, Barberry extract, lotus seed extract or hydrolysate thereof, fermented lotus seed product, Codonopsis ginseng extract or hydrolysate thereof, Job's tears hydrolysate, Job's tears seed fermented product, royal jelly fermented product, sake lees extract or ceramide contained therein, fermented sake lees product, Pandanus amaryllifolius (Pandanus amaryllifolius Roxb.) extract, Arcangelicia flava Merrilli extract, and chamomile extract.Also, coral grass extract, eggplant (water eggplant, long eggplant, Kamo eggplant, rice eggplant, etc.) extract or its hydrolysate, apricot fruit extract, seaweed extract such as Katamenkirinsai, extract of marine flowering plants such as Zostera marina, soybean extract or its hydrolysate, fermented soy milk, jellyfish water, rice extract or its hydrolysate, fermented rice extract, germinated rice extract or its hydrolysate, black soybean extract or its hydrolysate, Damask rose flower extract, bamboo shoot peel extract, linoleic acid and its derivatives or processed products (for example, liposomal linoleic acid, etc.), animal or fish-derived collagen and its derivatives, elastin and its derivatives, glycyrrhizic acid and its derivatives (dipotassium salt, etc.), t-cycloamino acid derivatives, vitamin A and its derivatives, allantoin, diisopropylamine dichloroacetate, gamma-amino-beta-hydroxybutyric acid, gentian extract, licorice extract, ginseng extract, Korean ginseng extract or its fermented product, red ginseng extract, aloe extract, kelp extract, loofah extract, Ulva pertusa extract, peach extract, peach kernel extract, kiwi extract, sunflower extract, Zizyphus joazeiro extract, pau d'arco bark extract, daylily extract or fermented product, wormwood extract, cherimoya extract, mango extract, mangosteen extract, funori extract, oolong tea extract, red fuki extract, purple orchid extract, extract or hydrolysate of Japanese pepper peel or seed coat, safflower flower extract, Casablanca extract, sweet potato extract or fermented product, grapefruit extract, guava leaf extract, Houttuynia cordata extract, Banpeiyu extract, apple extract, strawberry flower extract, etc.

[0037] Furthermore, examples of active agents that are preferably used in combination with the lactic acid bacteria fermentation product of rice according to the present invention include nicotinic acid or its derivatives, ascorbic acid or its derivatives, tranexamic acid, glycyrrhizinic acid or its derivatives, vitamin E and its derivatives (e.g., vitamin E nicotinate, vitamin E linoleate, etc.), etc.

[0038] The present invention will be explained in more detail below with reference to Production Examples, Examples, Test Examples and Formulation Examples, but the present invention is not limited thereto. In the following explanation, all % means % by weight.

[0039] Production Example 1. Lactic acid bacteria fermented rice (1) 10 kg of polished rice was washed with water and soaked in 2% fructose and 10% lactic acid bacteria (Lactobacillus plantarum). 8 The rice was placed in a fermentation tank together with 40 kg of a water dispersion of lactic acid bacteria (1000 kJ / mL) and subjected to lactic acid bacteria fermentation for 3 days at 37°C under a nitrogen atmosphere. After fermentation was completed, the fermented rice was washed with water at 40°C to remove lactic acid and protein. After washing, water equivalent to 50% of the fermented rice was added, and the rice was steamed at 90°C and dried. The resulting dried product was pulverized to obtain 8 kg of gelatinized lactic acid bacteria fermented rice powder.

[0040] Production Example 2. Lactic acid bacteria fermented rice (2) 10 kg of polished rice was washed with water and soaked in 2% fructose and 10% lactic acid bacteria (Lactobacillus plantarum). 8 The rice was placed in a fermentation tank together with 40 kg of a water dispersion of lactic acid bacteria (1000 kJ / mL) and fermented at 37°C for 3 days under a nitrogen atmosphere. After fermentation, water equivalent to 50% of the fermented rice was added, and the rice was steamed at 90°C and dried. The resulting dried product was pulverized to obtain 8 kg of gelatinized lactic acid bacteria-fermented rice powder.

[0041] Test example 1. Skin permeability test In this Test Example 1, the skin permeation-promoting effect of the rice lactic acid bacteria fermentation product of Production Example 1 on niacinamide and ascorbic acid derivatives (ascorbyl tetrahexyldecanoate, sodium ascorbyl phosphate, and ascorbic acid 2-glucoside), which are active ingredients for improving skin, was evaluated. First, an emulsion composition containing niacinamide or an ascorbic acid derivative (1) [ascorbyl tetrahexyldecanoate] shown in Table 1 was prepared. Next, a three-dimensional skin model TEST SKIN LSE-high (Roman Skin Lab Co., Ltd.) was placed on a medium, and 0.2 g of the emulsion composition was added to the stratum corneum of the three-dimensional skin model. After culturing for 2, 4, and 6 hours in this state, the medium on the bottom side of the three-dimensional skin model was collected, and the amount of niacinamide or ascorbic acid derivative (1) contained in the medium was analyzed using liquid chromatography (HPLC), and the cumulative amount (mg / cm) was calculated. 2) was graphed. [Table 1] JPEG0007759640000001.jpg84131 Also, an aqueous solution containing the ascorbic acid derivative (2) [sodium ascorbyl phosphate] or the ascorbic acid derivative (3) [ascorbic acid 2-glucoside] shown in Table 2 was prepared. Next, a three-dimensional skin model TEST SKIN LSE-high (Roman Skin Lab Co., Ltd.) was placed on the medium, and 0.2 g of the aqueous solution was added to the stratum corneum of the three-dimensional skin model. After culturing in this state for 2, 4, and 6 hours, the medium on the bottom side of the three-dimensional skin model was collected, and the amount of ascorbic acid derivative (2) or ascorbic acid derivative (2) contained in the medium was analyzed using liquid chromatography (HPLC), and the cumulative amount (mg / cm) was calculated. 2 ) was graphed. [Table 2] JPEG0007759640000002.jpg44129

[0042] The skin permeability evaluation results for Examples 1 and 2 and Comparative Examples 1 and 2 are shown in Figure 1. As shown in Figure 1, it was confirmed that the emulsion compositions of Examples 1 and 2, which contain the lactic acid bacteria fermentation product of rice from Production Example 1, significantly enhance the skin permeability of each of niacinamide and the ascorbic acid derivative (1), compared to the emulsion compositions of Comparative Examples 1 and 2, which do not contain the lactic acid bacteria fermentation product of rice from Production Example 1.

[0043] The permeability evaluation results for Examples 3-4 and Comparative Examples 3-4 are shown in Figure 2. As shown in Figure 2, it was confirmed that the aqueous solutions of Examples 3 and 4 containing the lactic acid bacteria fermentation product of rice of Production Example 1 significantly promoted the skin permeability of each of the ascorbic acid derivatives (2) and (3), compared to the aqueous solutions of Comparative Examples 3 and 4 not containing the lactic acid bacteria fermentation product of rice of Production Example 1. Similar results are also suggested for the lactic acid bacteria fermentation product of rice of Production Example 2. Furthermore, it is suggested that the lactic acid bacteria fermentation product of rice according to the present invention also has a skin permeability effect on active skin ingredients other than niacinamide or ascorbic acid derivatives (1)-(3), such as 3-O-ethyl ascorbic acid, tranexamic acid, glycyrrhizinic acid or a derivative thereof, D-pantothenyl alcohol, vitamin E and its derivatives (e.g., vitamin E nicotinate, vitamin E linoleate, etc.).

[0044] Test Example 2: Gene Expression Analysis Evaluation A three-dimensional skin model, TEST SKIN LSE-high (Roman Skin Lab, Inc.), was cultured according to standard methods. After 24 hours, 0.2 g of each of the emulsion composition of Example 1 and Comparative Example 1 was added to the tissue, followed by further culture for 1 day. A control model without any addition was also cultured in the same manner. After culture was completed, tissue from each test group was recovered with 1 mL of Trizol reagent (Invitrogen). 200 μL of chloroform (Wako Pure Chemical Industries, Ltd.) was added to the recovered tissue, mixed by stirring, and centrifuged at 15,000 rpm and 4°C for 15 minutes in a centrifuge (TOMY MX-160). 400 μL of the aqueous layer alone was then separated. 500 μL of isopropanol (Wako Pure Chemical Industries, Ltd.) was added to the recovered aqueous layer, mixed by stirring, and centrifuged at 15,000 rpm and 4°C for 15 minutes to obtain a total RNA precipitate. The total RNA was washed by adding 1 mL of 75% ethanol and vortexing. The mixture was then centrifuged at 15,000 rpm at 4°C for 15 minutes to recover the precipitate. The recovered total RNA was reverse-transcribed using a designated kit [PrimeScript RT reagent Kit with gDNA Eraser (Perfect Real Time) (Takara Bio)] to synthesize cDNA. Using this synthesized cDNA as a sample, expression of various target genes and the internal standard GAPDH gene were detected using Thermal Cycler Dice® Real Time System Single (Takara Bio) and SYBR® Premix ExTaq™ II (Perfect Real Time) (Takara Bio). GAPDH is a housekeeping gene (a gene expressed at a constant level in many tissues and cells, which is constantly expressed and essential for cell maintenance and proliferation). Because its expression level is consistently maintained, it is used as an internal standard in PCR experiments. The test results were obtained by comparing the expression levels of various target genes in each test group, with the expression level of the GAPDH gene held constant.In this test system, the expression level of each gene in the control group was set to 100, and the relative expression level of the gene in each group to which the emulsified composition of Example 1 and the emulsified composition of Comparative Example 1 were added was calculated.

[0045] The results of Test Example 2 are shown in Figure 3. As shown in Figure 3, compared to the emulsion composition of Comparative Example 1, the emulsion composition containing the lactic acid bacteria fermentation product of rice of Example 1 was confirmed to enhance the expression of filaggrin (FLG) and involucrin (IVL), which are involved in the barrier function of the epidermis; laminin α-chain (LAMA1), which is involved in the binding of the basement membrane and epidermis and is involved in epidermal turnover; hyaluronic acid synthase (HAS3); sphingomyelinase 1 (SMPD1), which is involved in ceramide synthesis; occludin (OCLN) and claudin (CLDN), which are involved in the synthesis of tight junctions; type I collagen α1 chain (COL1A1), which is involved in dermal firmness; type IV collagen α1 chain (COL4A1), a protein that constitutes the basement membrane and is involved in epidermal turnover; and the synthesis of type VII collagen, a protein that adheres the basement membrane to the dermis. This suggests that the lactic acid bacteria fermentation of rice in Production Example 1 exerts a synergistic effect of improving barrier function, hyaluronic acid synthesis, promoting ceramide synthesis, promoting type I collagen synthesis, and basement membrane care, due to its own effect and the effect of promoting the penetration of the skin active ingredient (niacinamide) used in combination, making it useful as an ingredient in topical skin preparations for improving wrinkles.

[0046] Prescription example 1. Emulsion [Ingredients] Part Jojoba oil 1.5 Squalane 2.0 Behenyl Alcohol 2.0 Hydrogenated Palm Oil 1.0 Fermented product of Production Example 1 2.0 Niacinamide 5.0 Carboxyvinyl polymer 0.15 Seaweed extract 0.2 Methylparaben 0.15 Ethylparaben 0.03 Purified water (enough to make the total volume 100 parts)

[0047] Prescription example 2: Emulsion [Ingredients] Part Olive S-decyl Esters 5.0 Squalene 5.0 Squalane 10.0 Glycerin 4.0 Xanthan gum 0.1 Fermented product of Production Example 2 2.0 Niacinamide 5.0 1,3-butylene glycol 3.0 Pentylene Glycol 3.0 Propanediol 2.0 Crystalline cellulose and cellulose gum 0.6 Phenoxyethanol 0.9 Purified water (enough to make the total volume 100 parts)

[0048] Prescription example 3. Emulsion [Ingredients] Part Liquid Paraffin 5.0 Olive oil 4.0 Squalane 5.0 Palmitic acid 2.5 Butylparaben 0.1 Fermented product of Production Example 1 2.0 1,3-butylene glycol 10.0 Ascorbic acid 2-glucoside 2.0 Potassium hydroxide 0.5 Locust bean gum 0.2 Methylparaben 0.1 Purified water (enough to make the total volume 100 parts)

[0049] Prescription example 4: Emulsion An emulsion was obtained in the same manner as in Formulation Example 3, except that 2.0 parts of sodium ascorbyl phosphate was used in place of 2.0 parts of L-ascorbic acid-2-glucoside contained in Formulation Example 3.

[0050] Prescription example 5. Emulsion An emulsion was obtained in the same manner as in Formulation Example 3, except that 2.0 parts of 3-O-ethyl ascorbic acid was used in place of 2.0 parts of L-ascorbic acid-2-glucoside contained in Formulation Example 3.

[0051] Prescription example 6. Emulsion An emulsion was obtained in the same manner as in Formulation Example 3, except that 2.0 parts of magnesium ascorbyl phosphate was used in place of 2.0 parts of L-ascorbic acid-2-glucoside contained in Formulation Example 3.

[0052] Prescription example 7. Cream [Ingredients] Part Olive oil 5.0 Squalane 5.0 Jojoba oil 5.0 Jojoba Wax 3.0 Behenyl Alcohol 1.0 Stearyl alcohol 1.5 Candelilla Wax 0.5 Fermented product of Production Example 1 2.0 Niacinamide 5.0 Hydrogenated lecithin 0.5 Carboxyvinyl polymer 0.3 Sodium alginate 0.2 Methylparaben 0.15 Ethylparaben 0.03 Purified water (enough to make the total volume 100 parts)

[0053] Prescription example 7. Cream [Ingredients] Part Squalane 3.0 Jojoba oil 5.0 Jojoba Wax 3.0 Shea Butter 5.0 Myristyl alcohol 1.0 Fermented product of Production Example 1 2.0 Niacinamide 5.0 Hydrogenated lecithin 0.5 Sodium hyaluronate 0.1 Xanthan gum 0.2 Glycerin 0.15 Pentanediol 4.0 Purified water (enough to make the total volume 100 parts)

[0054] Prescription example 8. Cream A cream was obtained in the same manner as in Formulation Example 7, except that 5.0 parts of niacinamide contained in Formulation Example 7 was replaced with 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 parts of potassium hydroxide.

[0055] Prescription example 9. Cream A cream was obtained in the same manner as in Formulation Example 7, except that 5.0 parts of niacinamide contained in Formulation Example 7 was replaced with 2.0 parts of ascorbyl tetrahexyldecanoate and 0.5 parts of potassium hydroxide.

[0056] Prescription example 10. Lotion [Ingredients] Part 0.5 tablespoons olive oil Squalane 0.5 Fermented product of Production Example 1 2.0 Ascorbic acid 2-glucoside 2.0 Potassium hydroxide 0.5 Xanthan gum 0.2 Ethanol 8.0 1,3-butylene glycol 5.0 Purified water (enough to make the total volume 100 parts)

[0057] Prescription example 11. Essence [Ingredients] Part Jojoba oil 0.5 Squalane 0.2 Fermented product of Production Example 2 2.0 Niacinamide 3.0 Sodium hyaluronate 0.1 Water-soluble collagen hydrolysate 0.1 Water-soluble collagen 1.0 Sodium alginate 0.2 Ethanol 5.0 1,3-butylene glycol 5.0 Methylparaben 0.2 Phenoxyethanol 0.5 Purified water (enough to make the total volume 100 parts)

[0058] Formulation example 12. Liquid foundation [Ingredients] Part Olive S-decyl Esters 5.0 Squalene 5.0 Squalane 10.0 Glycerin 4.0 Xanthan gum 0.1 Fermented product of Production Example 1 2.0 Niacinamide 3.0 1,3-butylene glycol 3.0 Pentylene Glycol 3.0 Propanediol 2.0 Crystalline cellulose and cellulose gum 0.6 Phenoxyethanol 0.9 Mica, Titanium Dioxide and Iron Oxide 2.5 Purified water (enough to make the total volume 100 parts)

[0059] Prescription example 13. Hair treatment [Ingredients] Part Stearyltrimethylammonium chloride 5.0 Glyceryl Monostearate 1.0 Cetyl alcohol 3.0 Octyldodecanol 2.0 Fermented product of Production Example 1 2.0 D-Pantothenyl alcohol 0.3 1,3-butylene glycol 5.0 Hydrolyzed collagen powder 5.0 Methylparaben 0.1 Purified water (enough to make the total volume 100 parts)

[0060] Prescription example 14. Hair cream [Ingredients] Part Squalane 15.0 Vaseline 15.0 Beeswax 2.0 Glycerin 3.0 1,3-butylene glycol 3.0 Xanthan gum 0.1 Fermented product of Production Example 2 0.5 Niacinamide 5.0 Polyoxyethylene hydrogenated castor oil 3.0 Methylparaben 0.2 Purified water (enough to make the total volume 100 parts)

[0061] Formulation example 15. Hair dye / dye base [A component] part Oxidative dyes 3.5 Oleic acid 20.0 Stearic acid diethanolamide 3.0 Polyoxyethylene (50) oleyl ether 1.0 Fermented product of Production Example 1 1.0 Niacinamide 5.0 1,3-butylene glycol 10.0 Isopropanol 10.0 Ammonia water (28%) 10.0 Sodium sulfite 0.5 Purified water (enough to make the total volume 100 parts)

[0062] Prescription example 16. Cream rinse [Ingredients] Part Polyoxyethylene (10) hydrogenated castor oil 1.0 Distearyldimethylammonium chloride 1.5 Stearyltrimethylammonium chloride 2.0 Glyceryl 2-ethylhexanoate 1.0 Cetyl alcohol 3.2 Stearyl Alcohol 1.0 Methylparaben 0.1 Fermented product of Production Example 2 1.0 D-Pantothenyl alcohol 0.3 1,3-butylene glycol 5.0 Purified water (enough to make the total volume 100 parts)

Claims

[Claim 1] A skin permeation enhancer for one or more of ascorbic acid, water-soluble ascorbic acid derivatives, and niacinamide, which contains rice fermentation product as an active ingredient.

Images