Fermented matter from structured water medium and cosmetic composition containing the same

Abstract

A cosmetic composition is disclosed, which comprises a fermentate from a fermentation mixture comprising at least one prebiotic active ingredient, a structured water component, and at least one probiotic microorganism. The fermentate may further comprise at least one cosmetic active ingredient. A method for increasing collagen production in the skin by applying the cosmetic composition is also disclosed.

Description

[Technical Field]

[0001] Cross-reference to related patent applications This application claims the benefit of priority from U.S. Provisional Patent Application No. 63 / 123,441, filed December 9, 2020, which is incorporated herein by reference in its entirety.

[0002] Technical Field The present disclosure relates to a fermentate obtained from a fermentation mixture comprising at least one prebiotic cosmetic ingredient, a structured water component, and at least one probiotic microorganism. The fermentate may further comprise at least one cosmetic active ingredient. The present disclosure also relates to a cosmetic composition comprising the fermentate. [Background technology]

[0003] Collagen, a component of the extracellular matrix, is the major matrix protein produced by skin fibroblasts. It is an important protein, accounting for approximately 30% of the total weight of biological proteins, and has a strong triple-helical structure. Collagen forms the majority of organic matter in skin, tendons, bones, and teeth, particularly in bone and skin (dermis). Most other sieve structures exist as fibrous inclusion bodies. Collagen's primary function is to contribute to the mechanical stiffness of skin. However, collagen decreases with age and photoaging due to ultraviolet radiation, which is known to be closely related to the formation of wrinkles in the skin. Collagen also plays an important role in wound healing by restoring damaged epithelium, allowing wounds to heal quickly and without leaving scars. Furthermore, collagen has excellent tensile strength and functions in a manner different from many other types of proteins. Collagen is found both inside and outside cells. Collagen fibers are important in contributing to the external structure of cells, providing tension and strength to the skin. However, as people age, collagen degradation occurs, collagen production decreases, and wrinkles occur. Collagen is therefore important for reducing wrinkles and the visible effects of aging on the skin.

[0004] Conventionally, to maximize the effects of collagen on the skin, products containing collagen in external cosmetic compositions have been used. However, when applied to the skin surface, such products are difficult to absorb through the skin, and they cannot achieve their transdermal effects. In addition, such products are limited in use due to problems such as irritation and redness, or have only a small cosmetic effect, and therefore do not achieve the expected effect of improving skin function. Therefore, there is an urgent need to develop a new cosmetic composition that is more effective than existing compositions and can promote collagen production.

[0005] Fermentation is a process of decomposing organic matter using microbial enzymes. Fermentation using microorganisms reduces toxicity levels, lowers molecular weight, or promotes percutaneous absorption, thereby improving bioavailability. Furthermore, the fermented substance itself exhibits enhanced effects through fermentation, thereby exerting effects not seen before fermentation. Summary of the Invention

[0006] The present disclosure is directed to novel ferments that are unexpectedly effective in antioxidant capacity, wrinkle reduction, skin lightening, skin brightening, and collagen production. The present disclosure is also directed to cosmetic compositions comprising the ferments.

[0007] overview A ferment is provided from a fermentation mixture comprising at least one prebiotic cosmetic ingredient, a structured water component, and at least one probiotic microorganism. The ferment may further comprise at least one cosmetic active ingredient.

[0008] The at least one prebiotic active ingredient may be selected from the group consisting of aloe, mango, aronia berry, raspberry, spinach, cherry broccoli, tart cherry, apple, mushroom, grape juice, beet, blueberry, blackberry, acai, coffee, ginger, cranberry, raspberry, aloe gel, coconut water, pomegranate, mango, apricot, retinol, raspberry, lavender, honey, cardamom, truffle, hyaluronic acid, and mixtures thereof.Preferably, the at least one prebiotic active ingredient may include aloe, grape juice, beet, retinol, lavender, honey, and hyaluronic acid.More preferably, the at least one prebiotic active ingredient may include aloe and hyaluronic acid.Most preferably, the at least one prebiotic active ingredient may include aloe.

[0009] A preferred aloe may include Aloe Barbadensis leaf powder, which may be present in an amount of about 0.001% to about 20% by weight, based on the total weight of the fermentation mixture.

[0010] The fermentation mixture may further include hyaluronic acid. The combined amount of Aloe barbadensis leaf powder and hyaluronic acid may be about 0.0001% to about 30% by weight, based on the total weight of the fermentation mixture.

[0011] The structural water component may include I water and S water.

[0012] The at least one probiotic microorganism may be a strain selected from the group consisting of Bifidobacterium, Lactobacillus, Enterococcus, Streptococcus, Staphylococcus, Saccharomyces cerevisiae, Saccharomyces boulardii, and mixtures thereof. Preferably, the at least one probiotic microorganism is a strain selected from the group consisting of Bifidobacterium and Lactobacillus. More preferably, the at least one probiotic microorganism is a Lactobacillus.

[0013] The fermentate may further comprise at least one cosmetic active ingredient. The at least one cosmetic active ingredient may be selected from the group consisting of Aloe barbadensis leaf polysaccharide, mango, aronia berry, raspberry, spinach, cherry broccoli, tart cherry, apple, mushroom, grape juice, beet, blueberry, blackberry, acai, coffee, ginger, cranberry, raspberry, aloe gel, coconut water, pomegranate, mango, apricot, retinol, raspberry, lavender, honey, cardamom, truffle, hyaluronic acid, and mixtures thereof. Preferred at least one cosmetic active ingredient may include Aloe barbadensis leaf polysaccharide, grape juice, beet, retinol, lavender, honey, and hyaluronic acid. More preferred at least one cosmetic active ingredient may include Aloe barbadensis leaf polysaccharide and hyaluronic acid. Most preferably, the at least one cosmetic active ingredient may include Aloe barbadensis leaf polysaccharides.

[0014] The fermentate may contain structured water. The structured water component may be present in the fermentate in an amount of about 1% to about 99.5% by weight based on the total weight of the fermentate. I water may be present in an amount of about 1% to about 60% by weight of the fermentate. S water may be present in an amount of about 1% to about 60% by weight of the fermentate.

[0015] The fermentate may be in the form of an extract, a lysate, or a filtrate.

[0016] Also provided is a cosmetic composition comprising a fermentate from a fermentation mixture comprising at least one prebiotic cosmetic ingredient, a structured water component, and at least one probiotic microorganism, wherein the fermentate may be present in an amount of from about 0.0001% to about 30% by weight, based on the total weight of the cosmetic composition.

[0017] The cosmetic composition is a skin lotion, a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a moisturizing lotion, a nourishing lotion, a massage cream, a facial mask, a facial cleanser, a nourishing cream, a moisturizing cream, a hand cream, a foundation, an essence, a nourishing essence, a cleansing foam, a cleansing lotion, a cleansing cream, a body lotion, a body mask, or a body cleanser.

[0018] A method for increasing collagen production in human skin is provided. The method includes applying a cosmetic composition containing a fermentation extract from a fermentation mixture containing at least one probiotic microorganism, a prebiotic active ingredient, and a structured water component. The most preferred prebiotic active ingredient may include Aloe barbadensis leaf powder. The structured water component may include I water and S water. The composition may be applied topically once, twice, or three times daily, or at night before bedtime. When applied topically, the composition may enhance skin hydration and moisturization for up to 100 hours, providing skin-brightening and skin-lightening benefits. DETAILED DESCRIPTION OF THE INVENTION

[0019] The dimensions and values ​​disclosed herein should not be understood as being strictly limited to the exact numerical values ​​recited. Instead, unless otherwise specified, each such dimension is intended to mean both the recited value and a functionally equivalent range surrounding that value. Except in the Examples and Comparative Examples, or where otherwise expressly indicated, all numbers in this description indicating amounts or ratios of ingredients, or reaction conditions, physical properties of ingredients, and / or uses, should be understood as modified by the word "about." Unless otherwise specified, all amounts are by weight of the final composition.

[0020] The term "active ingredient" or "active agent" or "cosmetic agent" refers to a cosmetic agent utilized to provide a benefit to the skin. An "active ingredient" or "active agent" or "cosmetic agent" will cause a change in the subject's skin or provide the benefit under consideration, thus helping to achieve a desired, expected, or intended result. The term "active ingredient" or "active agent" or "cosmetic agent" according to the present invention includes cosmetically acceptable excipients or carriers that may be present in a composition / formulation.

[0021] The terms "prevent" and "preventing" include preventing the recurrence, spread, or onset of a skin or hair condition. It is not intended that the present invention be limited to complete prevention.

[0022] The term "subject" refers to any mammal, preferably a human.

[0023] The term "topical" refers to the administration of one or more agents (eg, cosmetic agents, vitamins, etc.) to the skin.

[0024] The terms "transdermal" or "topical" refer to the delivery of an agent (e.g., a cosmetic agent, a dermatological agent, a vitamin, etc.) through the skin (e.g., such that at least a portion of the particle population reaches the underlying layers of the skin).

[0025] The terms "inhibiting," "reducing," or "prevention," or any variation of these terms, as used in the claims and / or this specification, include any measurable decrease or complete inhibition to achieve a desired result.

[0026] The term "effective," as used in the specification and / or claims, means sufficient to accomplish a desired, expected, or intended result.

[0027] The term "structured water," as used herein and / or in the claims, means water containing stabilized clusters of ions, as disclosed in U.S. Patent Nos. 6,139,855, 6,1356,30, 6,451,328, 6,905,523, and U.S. patent application Ser. Nos. 02 / 12,326, 11 / 673,119, and 11 / 421,834, the entire disclosures of which are incorporated herein by reference.

[0028] The term "I water," as used herein and / or in the claims, refers to the acidic fraction of structured water, which is disclosed in U.S. Patent Nos. 6,139,855, 6,1356,30, 6,451,328, 6,905,523, and U.S. patent application Ser. Nos. 02 / 12,326, 11 / 673,119, and 11 / 421,834, the entire disclosures of which are incorporated herein by reference.

[0029] The term "S water," as used herein and / or in the claims, refers to the basic fraction of structured water, which is disclosed in U.S. Patent Nos. 6,139,855, 6,1356,30, 6,451,328, 6,905,523, and U.S. patent application Ser. Nos. 02 / 12,326, 11 / 673,119, and 11 / 421,834, the entire disclosures of which are incorporated herein by reference.

[0030] The ferment can be produced from a fermentation mixture containing at least one prebiotic cosmetic ingredient, a structured water component, and at least one probiotic microorganism. Thus, the applicants have been able to achieve a novel ferment by utilizing the structured water component in the fermentation mixture.

[0031] The at least one prebiotic cosmetic ingredient may be selected from the group consisting of aloe, aronia berry, raspberry, spinach, cherry broccoli, tart cherry, apple, mushroom, grape juice, beet, blueberry, blackberry, acai, coffee, ginger, cranberry, raspberry, coconut water, pomegranate, mango, apricot, retinol powder, raspberry powder, lavender, honey, cardamom, truffle, hyaluronic acid, or mixtures thereof. Preferably, the at least one prebiotic cosmetic ingredient is derived from aloe. More preferably, the at least one prebiotic cosmetic ingredient is derived from Aloe barbadensis Miller.

[0032] When at least one prebiotic cosmetic ingredient is derived from a plant, the relevant plant part may be a leaf, stem, flower, root, seed, fruit, tuber, or coleoptile.Preferred plant parts may be leaves, stems, flowers, or fruits.More preferred plant parts may be leaves.

[0033] The at least one prebiotic cosmetic ingredient may be present in the form of a powder, liquid, semi-aqueous liquid, or concentrated form, with the preferred form being powder.

[0034] The most preferred prebiotic cosmetic ingredient is Aloe barbadensis leaf powder.

[0035] The prebiotic cosmetic ingredient may be present in the fermentation mixture in an amount ranging from about 0.00001% to about 20%, preferably from about 0.0001% to about 10%, more preferably from about 0.001% to about 5%, and most preferably from about 0.01% to about 3%, based on the total weight of the fermentation mixture, all percentages referred to herein being by weight.

[0036] Aloe barbadensis leaf powder can be produced by processing Aloe barbadensis Miller leaves within four hours of harvest. The inner fillet of the aloe is extracted and the fibers removed, while the aloin is removed by charcoal absorption, filtered, pasteurized, and concentrated. The concentrate is then spray-dried to a powder form with a moisture content of less than 7%. The powder contains no preservatives or matrices and contains at least 10% polysaccharides (e.g., without limitation, starch, cellulose, glycogen, peptidoglycan, etc.).

[0037] Structural water components are described in U.S. Patent Nos. 6,139,855, 6,135,630, 6,451,328, 6,905,523, and U.S. Patent Application Nos. US02 / 12326, 11 / 673,119, and 11 / 421,834, all of which are incorporated herein in their entireties. The structural water component can include I water and S water.

[0038] Structured water is defined as water containing stabilized clusters of ions. Structured water I (or "I water") is the acid fraction containing stabilized clusters of R+(H)- (Cl-, PO4 3-, SO4 2-) ions. Structured water S (or "S water") is the basic fraction containing stabilized clusters of R+(OH)-n (Ca2+, Mg2+, Na+, K+, etc.) ions. Preferably, I water is characterized by a conductivity of about 500-3000 μS and a pH of about 2.0-3.0, and S water is characterized by a conductivity of about 600-2500 μS and a pH of about 10-12, each resulting from starting water having a conductivity of about 250-450 μS / cm and a pH of about 7-7.5.

[0039] The structured water component may be present in the fermentation mixture in an amount ranging from about 80% to about 99.999%, preferably from about 90% to about 99.99%, more preferably from about 95% to about 99.9%, and most preferably from about 98% to about 99%, based on the total weight of the fermentation mixture, all percentages referred to herein being by weight.

[0040] It is most unexpected that by using structured water in the fermentation medium, surprising beneficial efficiencies can be achieved.

[0041] It was also unexpected that the beneficial effectiveness of the ferment did not result from the structured water that became the final ferment.

[0042] The probiotic microorganism(s) may be included in the form of a fraction of cellular components. The probiotic microorganism(s) or fraction(s) may also be introduced into the fermenter in the form of a lyophilized powder, a culture supernatant, and / or, if appropriate, in a concentrated form.

[0043] The at least one probiotic microorganism may be from the class Ascomycetes, such as Bifidobacterium, Yarrowia, Kluyveromyces, Torulaspora, Schizosaccharomyces pombe, Debaromyces, Candida, Pichia, Aspergillus, and Penicillium, the genus Bifidobacterium, Bacteroides, Fusobacterium, Melissococcus, Propionibacterium, Enterococcus, Lactococcus, or the like. The bacterial strain may be selected from the group consisting of bacteria of the genera Pediococcus, Staphylococcus, Peptostrepococcus, Bacillus, Pediococcus, Micrococcus, Leuconostoc, Weissella, Aerococcus, Oenococcus and Lactobacillus, or a mixture thereof.

[0044] Probiotic microorganisms also include, but are not limited to, genus bacteria and yeasts, such as lactic acid bacteria, species of Lactobacillus, which produce lactic acid by fermentation of sugars, e.g., Lactobacillus acidophilus Lactococcus acidophilus, Amylovorus, casei, Rhamnosus, brevis, crispatus, delbrueckii (subspecies bulgaricus, lactis), fermentum, helveticus, gallinarum, gasseri, johnsonii, paracasei, plantarum, reuteri, salivarius, alimentarius, curvatus, casei (subspecies casei, sake), Gocci species, e.g. Lactococcus lactis lactis (subspecies lactis or cremoris), Leuconstoc mesenteroides (subspecies Dextranicum), Pediococcus acidilactici, Sporolactobacillus inulinus, Streptococcus salvarius (subspecies Thermophilus), Streptococcus thermophilus, Staphylococcus carnosus, Staphylococcus xylosus xylosus, Bifidobacteria or Bifidobacterium species, e.g. Bifidobacterium adressentisadolescentis, animalis, bifidum, breve, lactis, longum, infantis, pseudocatenulatum, yeast such as Saccharomyces (cerevisiae or boulardii), or other spore-forming bacteria such as Bacillus (cereus var toyo or subtilis), Bacillus coagulans, Bacillus licheniformis, Escherichia coli strain nissle, Propionibacterium freudenreichii, or mixtures thereof.

[0045] Preferably, the at least one probiotic microorganism may be a strain selected from the group consisting of the genera Bifidobacterium and Lactobacillus. More preferably, the at least one probiotic microorganism may be from the genus Lactobacillus.

[0046] Examples of Lactobacillus species include, but are not limited to, Lactobacillus, Lactobacillus acidophilus, Lactobacillus amylovorus, Lactobacillus casei, Lactobacillus rhamnosus, Lactobacillus brevis, Lactobacillus crispatus, Lactobacillus delbreckii, Lactobacillus fermentum, Lactobacillus helveticus, Lactobacillus gallinarum, Lactobacillus gasseri, Lactobacillus johnsonii, Lactobacillus paracasei, Lactobacillus plantarum, Lactobacillus reuteri, Lactobacillus salivarius, Lactobacillus alimentarius, Lactobacillus culvertus, Lactobacillus casei, Lactobacillus salmonis, Lactococcus lactis, Leuconostoc mesenteroides, Pediococcus acidilactici, Sporolactobacillus inulinus, Streptococcus salvarius, Streptococcus thermophilus, Staphylococcus carnosus, Staphylococcus xylosus, Saccharomyces (cerevisiae or boulardii), Bacillus (cereus var. toyo or subtilis), Bacillus coagulans, Bacillus licheniformis, Escherichia coli, Propionibacterium früdenreichii, or a mixture thereof. Preferred species are Lactobacillus johnsonii, Lactobacillus paracasei, Bifidobacterium alesentis, Bifidobacterium longum and Bifidobacterium lactis, or mixtures thereof.

[0047] Examples of Bifidobacterium include, but are not limited to, Adolescentis, Longum, and Lactis.

[0048] The probiotic microorganisms may be present in the fermentation mixture in an amount ranging from about 0.001% to about 5%, preferably from about 0.005% to about 1%, more preferably from about 0.01% to about 0.5%, and most preferably from about 0.05% to about 0.15%, based on the total weight of the fermentation mixture, all percentages referred to herein being by weight.

[0049] The fermentate may further comprise at least one cosmetic active ingredient. The at least one cosmetic active ingredient may be selected from the group consisting of Aloe barbadensis leaf polysaccharide, mango, aronia berry, raspberry, spinach, cherry broccoli, tart cherry, apple, mushroom, grape juice, beet, blueberry, blackberry, acai, coffee, ginger, cranberry, raspberry, aloe gel, coconut water, pomegranate, mango, apricot, retinol, raspberry, lavender, honey, cardamom, truffle, hyaluronic acid, and mixtures thereof. Preferred at least one cosmetic active ingredient may include Aloe barbadensis leaf polysaccharide, grape juice, beet, retinol, lavender, honey, and hyaluronic acid. More preferred at least one cosmetic active ingredient may include Aloe barbadensis leaf polysaccharide and hyaluronic acid. Most preferably, the at least one cosmetic active ingredient is Aloe barbadensis leaf polysaccharide.

[0050] Non-limiting examples of suitable Aloe barbadensis leaf polysaccharides include those commercially available from Ashland Inc. under the trade name ALOE VERA IL SD PWD 200X 705AV ORGANIC.

[0051] The cosmetic active ingredient may be present in the ferment in an amount ranging from about 0.000001% to about 10%, preferably from about 0.00001% to about 1%, more preferably from about 0.00005% to about 0.1%, and most preferably from about 0.0001% to about 0.01%, based on the total weight of the ferment, all percentages referred to herein being by weight.

[0052] At least one cosmetic active ingredient may be introduced into the ferment in the form of a solution of the cosmetic active ingredient in structured water. The cosmetic active ingredient may be present in the structured aqueous solution in an amount ranging from about 0.0001% to about 20%, preferably from about 0.001% to about 10%, more preferably from about 0.005% to about 1%, and most preferably from about 0.01% to about 0.1%, based on the total weight of the solution of the cosmetic active ingredient in structured water, all percentages referred to herein being by weight.

[0053] The fermentate may be in the form of a lysate, extract, filtrate, or both. In the case of a lysate, the fermentation product is dissolved. In the case of a filtrate or extract, the fermentation product is filtered. The preferred form of the fermentate is a filtrate.

[0054] The method for producing the ferment may include the following steps: 1) preparing a fermentation mixture by mixing at least one prebiotic cosmetic ingredient, a structured water component, and at least one probiotic microorganism; 2) stirring the fermentation mixture at a temperature between 20°C and 30°C, preferably at about 25°C, for a period of 1 to 10 days, preferably 2 to 8 days, more preferably 3 to 5 days; 3) increasing the temperature to between 40°C and 50°C, preferably about 45°C, and maintaining the temperature for a period of 12 to 36 hours, preferably about 24 hours; 4) 3) filtering the resulting mixture, and 5) optionally adding a structured aqueous solution or suspension of at least one cosmetic active ingredient to the filtrate resulting from step 4), wherein the structured aqueous solution or suspension of at least one cosmetic active ingredient comprises the at least one cosmetic active ingredient in an amount ranging from about 0.0001% to about 20%, preferably from about 0.001% to about 10%, more preferably from about 0.005% to about 1%, and most preferably from about 0.01% to about 0.1% (w / w) based on the total weight of the structured aqueous solution or suspension. Preferably, the method for producing a fermentate includes step 5). More preferably, the structured aqueous solution or suspension of at least one cosmetic active ingredient is a solution.

[0055] In optional step 5) of the method for producing a fermentate, the amount of the aqueous structured solution of at least one cosmetic active ingredient added may range from about 0.01% to about 20%, preferably from about 0.1% to 10%, more preferably from about 0.5% to 8%, and most preferably from about 1% to about 5% (w / w) based on the total weight of the fermentation mixture of step 1).

[0056] Also provided herein is a cosmetic composition comprising a fermentate. The fermentate is produced from a fermentation mixture comprising at least one prebiotic cosmetic ingredient, a structured water component, and at least one probiotic microorganism, the structured water component comprising I water and S water. Preferably, the fermentate may further comprise at least one cosmetic active ingredient.

[0057] Specifically, the cosmetic composition provides improved penetration of cosmetic ingredients into the layers of the skin, enhances skin hydration, provides skin whitening and brightening benefits, improves skin integrity, and improves the bioavailability of cosmetic products.

[0058] The cosmetic composition may contain the fermentant in an amount ranging from about 0.00001% to about 20%, preferably from about 0.0001% to about 15%, more preferably from about 0.001% to about 10%, and most preferably from about 0.001% to about 5% (w / w) based on the total weight of the composition.

[0059] The cosmetic composition may contain at least one cosmetic active ingredient in an amount ranging from about 0.0001% to about 20% by weight, based on the total weight of the fermentate. In yet other compositions, the prebiotic-containing fermentate may be formulated to contain an effective amount of another cosmetic active ingredient, such as hyaluronic acid. In such compositions, the combined amount of the prebiotic cosmetic ingredient and the other cosmetic ingredient, such as, but not limited to, hyaluronic acid, ranges from about 0.00001% to about 30% by weight, based on the total weight of the composition. Furthermore, such compositions may contain a combination of cosmetic ingredients in an amount ranging from about 0.0001% to about 20% by weight, based on the total weight of the composition.

[0060] The cosmetic composition can be used in any topically applied skin care product that has an aqueous medium component. For example, structured water can be used in a completely aqueous culture medium, a hydroalcoholic medium, or as part of the aqueous phase of a water-in-oil or oil-in-water emulsion. The composition may also include a vehicle suitable for topical application to the skin, such as a solution, colloidal dispersion, emulsion, suspension, cream, lotion, gel, foam, mousse, spray, etc. The type of cosmetic active substance and the activity enhanced by the presence of I water and S water can be any that is beneficially used in skin care products. For example, structured water is useful for enhancing the moisturizing properties of a fermentation extract containing a moisturizing active substance. The fermented extract may also be used to enhance the activity of pharmaceutical or cosmetic actives used to treat age spots, keratosis, and wrinkles, as well as analgesics, anesthetics, anti-acne agents, antibacterial agents, anti-yeast agents, anti-fungal agents, antioxidants, anti-viral agents, anti-dandruff agents, anti-dermatitis agents, anti-pruritics, anti-emetic agents, anti-motion sickness agents, anti-irritants, anti-inflammatory agents, anti-hyperkeratolytic agents, anti-dry skin agents, antiperspirants, anti-psoriasis agents, anti-seborrheic agents, hair conditioners and treatments, anti-aging agents, anti-wrinkle agents, sunscreen agents, antihistamines, skin lightening agents, depigmenting agents, wound healing agents, vitamins, corticosteroids, self-tanning agents, or hormones. All cosmetic actives that enhance such benefits are contemplated to be utilized as fermentation media with the structured water described herein and are therefore contemplated to be within the scope of the present disclosure.

[0061] The I / S water medium can be used in amounts of about 1% to about 99.5% by weight of the total ferment, but is more frequently used at levels of about 20% to about 80%, or even about 40% to about 80%. The ferment, including the I / S water, itself can constitute the entire aqueous component of the composition. Alternatively, the I and S combination can be part of the aqueous component of the fermented medium, i.e., it can be further combined with other unstructured aqueous components, such as distilled water or floral water. The enhancing effect of the I / S combination has been observed even at dilutions of 1 / 1000, allowing for the use of unstructured water in combination with structured water in the fermented medium.

[0062] Structured water is the fermentation medium and may additionally be present in the composition. In addition to I water and S water, structured water further comprises salts. Such salts provide salinity, osmolality, or osmolality to the fermentation medium. The combination of I water and S water with salts in specific ratios / amounts creates a unique environment within the fermentation medium for microorganisms to survive and grow. As a result, the microorganisms can effectively metabolize the prebiotic active ingredients present in the medium, thereby creating a unique postbiotic fermentation medium containing multiple effective metabolic products or intermediates. Such metabolic products and any intermediate fermentation products are contemplated as being within the scope of this disclosure.

[0063] The ferment can be used in any type of skin care or makeup product that has an aqueous component. For example, it can be used to enhance the properties of active ingredients used in makeup products such as lipsticks and glosses, foundations, primers, blushers, eyeliners, eyeshadows, etc. It is also useful in treatment products where the effectiveness of the active ingredient is particularly desired.

[0064] Thus, the ferment described herein is produced from a fermentation mixture containing at least one prebiotic cosmetic ingredient, a structured water component, and at least one probiotic microorganism. Optionally, the ferment may contain any cosmetically acceptable excipient. The ferment provides a unique postbiotic mixture that exhibits increased bioavailability, penetration, and efficacy on human skin. Cosmetic compositions containing such ferment are used for external topical administration and may be in the form of aqueous, aqueous-alcoholic, or oily solutions, solution-type dispersions, lotion- or serum-type dispersions, milk-type emulsions with liquid or semi-liquid consistency, cream-type suspensions or emulsions, aqueous or anhydrous gels, microemulsions, microcapsules, microparticles, or ionic and / or nonionic vesicular dispersions.

[0065] Therefore, the present applicants have developed cosmetic compositions or products containing novel fermentates. These compositions have been shown to provide effective collagen-producing activity, non-cytotoxic anti-aging activity, and skin lightening and brightening benefits. Specifically, fermentate-containing compositions overcome issues with active bioavailability, allowing for maximum effectiveness on the skin. The novel fermentates have been shown to increase collagen production by approximately 48% in vitro. Furthermore, fermentate-containing compositions have been shown to provide supercharged hydration, penetrating multiple layers deep into the skin surface. The compositions also activate the skin's own moisture reservoirs for hydration, which lasts for up to 100 hours, maximizing hydration.

[0066] The method of use for the composition depends on the ultimate intended use of the composition. For example, the compositions described herein may be applied to the skin periodically at night and during the day. The compositions may also be applied to the skin periodically once, twice, or three times a day for about 1 to 8 weeks, or for as many weeks as needed or desired.

[0067] Additional ingredients The compositions herein may further contain other additional ingredients, which may be selected by those skilled in the art according to the desired characteristics of the final product, and are suitable for making the compositions more cosmetically or aesthetically acceptable or providing them with additional benefits in use.The ingredients useful herein are conveniently classified according to their specific benefits or their assumed mechanisms of action, but the given categories do not limit their use.Furthermore, it is understood that one ingredient may provide multiple benefits.

[0068] The CTFA Cosmetic Ingredient Handbook, 10th Edition (published by the Cosmetic, Toiletry, and Fragrance Association, Inc., Washington, DC) (2004) (hereinafter "CTFA") describes a wide variety of non-limiting materials that can be added to the compositions herein. Examples of these ingredient classes include, but are not limited to, abrasives, absorbents, aesthetic ingredients such as fragrances, pigments, colorants / colorants, essential oils, skin sensates, astringents, including cosmetic and medicated astringents (e.g., clove oil, menthol, camphor, eucalyptus oil, eugenol, menthyl lactate, witch hazel distillate), anti-acne agents, anti-caking agents, anti-foaming agents, antimicrobial agents (e.g., iodopropynyl butylcarbamate), antibacterial agents, anti-fungal ... Antifungal agents, antioxidants, binders, biological additives, buffers, bulking agents, chelating agents, chemical additives, colorants, cosmetic biocides, denaturants, topical analgesics, film-forming agents or materials to aid in the film-forming properties and durability of the composition, such as polymers (e.g., copolymers of eicosene and vinylpyrrolidone), opacifying agents, pH adjusters, plant derivatives, plant extracts, plant tissue extracts, plant seed extracts, plant oils, preservatives, propellants, reducing agents, sebum control agents, and sequestering agents. Other optional ingredients that can be incorporated into the compositions described herein include, but are not limited to, one or more cosmetic skin care agents. A cosmetic skin care agent is any substance, material, or compound intended to be applied to the skin for the purpose of improving an undesirable skin condition (or its symptoms). Some undesirable skin conditions include not only external visually and tactilely perceptible manifestations, but also any other macro- or micro-effects resulting from skin aging. Such symptoms may be induced or caused by endogenous or exogenous factors, such as aging over time and / or environmental insults.These signs include the development of discontinuities in texture, such as wrinkles, folds, skin lines, fissures, ridges, large pores (e.g., associated with appendage structures such as sweat ducts, sebaceous glands, or hair follicles), scaly, flaky, and / or other forms of unevenness or roughness of the skin, loss of skin elasticity (loss and / or inactivation of functional skin elastin), sagging (including swelling in the eye area and jawline), loss of skin turgor, loss of skin firmness, and skin irritation. Loss of firmness, loss of skin rebound from deformation, discoloration (including dark circles under the eyes), age spots, paleness, hyperpigmented skin areas such as age spots and freckles, can result from processes including, but not limited to, keratosis, abnormal differentiation, hyperkeratinization, elastosis, collagen degradation, and other histological changes in the stratum corneum, dermis, epidermis, dermal vasculature (e.g., telangiectasias or spider vessels), and underlying tissues, particularly those closest to the skin.

[0069] The composition may include a dermatologically or cosmetically acceptable carrier or excipient. Thus, the carrier may function as a diluent, dispersant, solvent, etc. for the peptide and other materials, compounds, and / or drugs. Exemplary acceptable excipients include any solvent, dispersion medium, diluent, or other liquid vehicle, dispersion or suspension aid, surfactant, isotonicity agent, thickener or emulsifier, preservative, solid binder, lubricant, suitable for topical administration and dosing. Except insofar as any conventional carrier medium is incompatible with the substance or its derivatives, for example, by producing any undesirable biological effects or otherwise interacting in a deleterious manner with any other component(s) of the composition, its use is contemplated within the scope of the present invention. The carrier may contain one or more acceptable solid, semi-solid, or liquid fillers, diluents, solvents, extenders, etc. The carrier may be solid, semi-solid, or liquid. The carrier itself may be inert, or it may have its own dermatological or cosmeceutical benefits. The concentration of the carrier can vary with the carrier selected and the intended concentrations of the essential and optional ingredients. In the composition, the carrier is present at a level of about 50% to about 99.99% (e.g., about 60% to about 99.9%, or about 70% to about 98%, or about 80% to about 95%) by weight of the composition. Acceptable carriers can be provided in a wide variety of forms. Non-limiting examples include, but are not limited to, simple solutions (water or oil), emulsions, and solid or semi-solid forms (gels, sticks). For example, emulsion carriers include, but are not limited to, oil-in-water, water-in-oil, water-in-silicone, water-in-oil-in-water, and oil-in-water-in-silicone emulsions. As will be understood by those skilled in the art, a given ingredient will be distributed primarily in either the aqueous or oil phase depending on the water solubility / dispersibility of the ingredient in the composition. In some embodiments, the personal care compositions described herein are formulated into an oil-in-water emulsion.

[0070] Suitable carriers also include oils. The compositions may contain from about 1% to about 95% by weight of one or more oils. The compositions may contain from about 0.1%, 0.5%, 1%, 2%, 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, or 90% to about 90%, 85%, 80%, 75%, 70%, 65%, 60%, 55%, 50%, 45%, 40%, 35%, 30%, 25%, 20%, 15%, 10%, 5%, or 3% of one or more oils. Oils may be used to solubilize, disperse, or retain materials that are incompatible with water or aqueous solvents. Suitable oils include silicones, hydrocarbons, esters, amides, ethers, and mixtures thereof. The oil may be fluid at room temperature. The oil may be volatile or non-volatile. "Non-volatile" means that the material exhibits a vapor pressure of about 0.2 mm Hg or less at 25°C under 1 atmosphere and / or a boiling point of at least about 300°C under 1 atmosphere. "Volatile" means that the material exhibits a vapor pressure of at least about 0.2 mm Hg at 20°C. When a heavy, oily film is not desired, volatile oils can be used to provide a lighter feel. When the skin care composition is in the form of an emulsion, the oil is typically a carrier associated with the oil phase. The composition may also include an emulsifier. An emulsifier is particularly suitable when the composition is in the form of an emulsion or when immiscible materials are combined. The skin care composition may contain from about 0.05%, 0.1%, 0.2%, 0.3%, 0.5%, or 1% to about 20%, 10%, 5%, 3%, 2%, or 1% of an emulsifier. The emulsifier may be nonionic, anionic, or cationic. The compositions described herein may be in the form of a pourable liquid (under ambient conditions). Thus, the composition may include an aqueous carrier, typically present at a level of about 20% to about 95% (or about 60% to about 85%) by weight of the composition. The aqueous carrier may include water or a miscible mixture of water and an organic solvent, but preferably includes water with minimal or no significant concentrations of organic solvent, except when incidentally incorporated into the composition as a minor component of other essential or optional ingredients.

[0071] The compositions of the present invention also include a carrier, present at a level of about 20% to about 99.99%, about 30% to about 90%, or about 40% to about 80% by weight of the composition. The carrier may be in a wide variety of forms. Non-limiting examples include simple solutions (e.g., aqueous, organic solvent, or oil-based), emulsions, suspensions, and solid forms (e.g., gels, sticks, flowable solids, or amorphous materials). In certain embodiments, the dermatologically acceptable carrier is in the form of an emulsion or suspension. Emulsions or suspensions can generally be classified as having a continuous aqueous phase (e.g., oil-in-water and water-in-oil-in-water) or a continuous oil phase (e.g., water-in-oil and oil-in-water-in-oil). The oil phase of the present invention may include silicone oils, non-silicone oils, such as hydrocarbon oils, esters, ethers, and the like, and mixtures thereof.

[0072] The emulsion may further comprise an emulsifier. The composition may contain any suitable percentage of emulsifier to fully emulsify the carrier. Suitable weight ranges include about 0.1% to about 10% or about 0.2% to about 5% emulsifier by weight of the composition. The emulsifier may be nonionic, anionic, or cationic. Suitable emulsifiers are disclosed, for example, in U.S. Pat. Nos. 3,755,560, 4,421,769, and McCutcheon's Detergents and Emulsifiers, North American Edition, pp. 317-324 (1986). Suitable emulsions may have a wide range of viscosities, depending on the desired product form. The carrier may further comprise a thickener, as is known in the art, to provide a composition with appropriate viscosity and rheological properties.

[0073] Transdermal Delivery Vehicles Transdermal delivery vehicles may include physical or chemical mechanisms designed to deliver materials beneath the skin's surface. Examples of such physical mechanisms include known penetration-enhancing cosmetic compositions or ingredients, hypodermic needles, microneedles, transdermal patches, electrospun nanofibers, and the like. Other delivery systems may include, for example, the use of chemical enhancers, non-cavitating ultrasound, iontophoresis, and other energy devices. The compositions herein may further include at least one film-forming polymer. The film-forming polymer may be selected from cellulose polymers, such as nitrocellulose, cellulose acetate, cellulose acetate / butyrate, cellulose acetate / propionate, and ethylcellulose; polyurethanes; acrylic polymers; vinyl polymers; polyvinyl butyral; alkyd resins; resins derived from aldehyde condensation products, such as arylsulfonamide-formaldehyde resins, e.g., toluenesulfonamide-formaldehyde resins, and arylsulfonamide-epoxy resins. Further non-limiting examples of suitable film-forming polymers include nitrocellulose from Hercules, toluenesulfonamide-formaldehyde resin "Ketjentflex MS80" from Akzo, "Santolite MHP," "Santolite MS 80," and "Resimpol 80" from Pan Americana, alkyd resin "Beckosol ODE 230-70-E" from Dainippon, acrylic resin "Acryloid B66" from Rohm & Haas, and polyurethane resin "Trixene PR 4127" from Baxenden. The film-forming polymer may generally be present at from about 1% to about 50%, preferably from about 2% to about 40%, and most preferably from about 2% to about 35% of the composition.

[0074] Antioxidants and radical scavengers Antioxidants and radical scavengers are particularly useful for providing protection against UV radiation, which can cause increased scaling or texture changes in the stratum corneum, and against other environmental factors that can cause skin damage. Such antioxidants / radical scavengers include, for example, tocopherol sorbate and other esters of tocopherol, and tocopherol sorbate.

[0075] anti-inflammatory agents Anti-inflammatory agents enhance skin appearance benefits, for example, by contributing to evenness and acceptable skin tone and / or color. Optionally, anti-inflammatory agents include steroidal and non-steroidal anti-inflammatory agents. The steroidal anti-inflammatory agent may be hydrocortisone. So-called "natural" anti-inflammatory agents are also useful. For example, alpha bisabolol, aloe vera, manjistha (extracted from plants of the Rubia genus, particularly Rubia Cordifolia), guggal (extracted from plants of the Commiphora genus, particularly Commiphora Mukul), kola extract, chamomile, and sagebrush extract may also be used.

[0076] antimicrobial agents As used herein, "antimicrobial agent" refers to a compound capable of destroying microorganisms, preventing the development of microorganisms, or preventing the pathogenic action of microorganisms. Antimicrobial agents are useful, for example, in controlling acne. Preferred antimicrobial agents are benzoyl peroxide, erythromycin, tetracycline, clindamycin, azelaic acid, sulfur resorcinol, phenoxyethanol, and Irgasan™ DP 300 (Ciba Geigy Corp., USA). A safe and effective amount of antimicrobial agent may be added to the emulsions herein, preferably 0.001% to 10%, more preferably 0.01% to 5%, and even more preferably 0.05% to 2%.

[0077] chelating agents As used herein, "chelating agent" refers to a compound that reacts to remove metal ions from a system by forming a complex so that the metal ions cannot readily participate in or catalyze chemical reactions. The inclusion of a chelating agent is particularly useful for providing protection against UV radiation, which can contribute to excessive scaling or changes in skin texture, and against other environmental factors that can cause skin damage. Exemplary chelating agents useful herein are disclosed in U.S. Patent No. 5,487,884, issued January 30, 1996, to Bissett et al., and PCT Application Nos. 91 / 16035 and 91 / 16034, published October 31, 1995, to Bush et al. A preferred chelating agent is furildioxime and its derivatives.

[0078] Silicone elastomer The composition may also contain a non-emulsifying crosslinked organopolysiloxane elastomer. As used herein, the term "non-emulsifying" defines a crosslinked organopolysiloxane elastomer that is free of polyoxyalkylene units. Such elastomers are used to reduce the sticky / greasy feeling associated with skin conditioning agents.

[0079] The elastomer may be dimethicone / vinyl dimethicone crosspolymer, vinyl dimethicone / lauryl dimethicone crosspolymer, C30-C45, alkyl cetearyl dimethicone / polycyclohexane oxide crosspolymer, and mixtures thereof.

[0080] Dimethicone / vinyl dimethicone crosspolymers are supplied by a variety of suppliers, including Dow Corning (DC 9040 and DC 9041), General Electric (SFE 839), Shin Etsu (KSG-15, 16, 18 [dimethicone / phenyl vinyl dimethicone crosspolymer]), and Grant Industries (GRANSIL™ line of elastomers). Crosslinked organopolysiloxane elastomers and methods for their manufacture are further described in U.S. Pat. No. 4,970,252 to Sakuta et al., issued November 13, 1990; U.S. Pat. No. 5,760,116 to Kilgour et al., issued June 2, 1998; and U.S. Pat. No. 5,654,362 to Schulz, Jr. et al., issued August 5, 1997.

[0081] Vinyl dimethicone / lauryl dimethicone crosspolymers include vinyl dimethicone / lauryl dimethicone crosspolymer & mineral oil (trade name KSG-41), vinyl dimethicone / lauryl dimethicone crosspolymer & isododecane (trade name KSG-42), vinyl dimethicone / lauryl dimethicone crosspolymer & triethylhexanoin (trade name KSG-43), and vinyl dimethicone / lauryl dimethicone crosspolymer & squalane (trade name KSG-44). Each of these silicone elastomers designated "KSG" is available from Shinestu Chemical.

[0082] Commercially available cyclomethicone and C30-C45 alkyl cetearyl dimethicone / polycyclohexane oxide crosspolymer is available from GE Silicone under the tradename Velvasil 125.

[0083] Whitening agent The compositions herein may further comprise 0.001% to 10%, or 0.1% to 5%, of a whitening agent. Non-limiting examples of suitable whitening agents are those that are compatible with aqueous compositions. The whitening agent may include active ingredients that not only change the appearance of the skin compared to before treatment, but also improve hyperpigmentation.

[0084] Useful whitening agents include ascorbic acid compounds, azelaic acid, butylhydroxyanisole, gallic acid and its derivatives, glycyrrhizic acid, hydroquinoine, kojic acid, arbutin, mulberry extract, and mixtures thereof. The use of combinations of whitening agents is believed to be advantageous in that they may provide whitening benefits through different mechanisms.

[0085] The ascorbic acid compound may be an ascorbate salt or a derivative thereof. Exemplary water-soluble salt derivatives include, but are not limited to, L-ascorbic acid 2-glucoside, L-ascorbyl phosphate salts, such as sodium L-ascorbyl phosphate, potassium L-ascorbyl phosphate, magnesium L-ascorbyl phosphate, calcium L-ascorbyl phosphate, and aluminum L-ascorbyl phosphate. L-ascorbyl sulfate salts may also be used. Examples include sodium L-ascorbyl sulfate, potassium L-ascorbyl sulfate, magnesium L-ascorbyl sulfate, calcium L-ascorbyl sulfate, and aluminum L-ascorbyl sulfate.

[0086] pH adjuster The composition may further comprise a pH adjuster to control the pH of the composition. In particular, the pH of the composition of the present disclosure is within the range of about 5 to about 8, or about 5.2 to about 7.8, or about 5.4 to about 7.6, for example, about 5.4, about 5.6, about 5.8, about 6.0, about 6.2, about 6.4, about 6.6, about 6.8, about 7.0, about 7.2, about 7.4, about 7.6, and any range therebetween.

[0087] The composition may further comprise about 0.01% to about 5%, or about 0.1% to about 3%, or about 0.3% to about 2%, or about 0.4% to about 1.8%, and / or about 0.5% to about 1.6%, e.g., about 0.4%, about 0.5%, about 0.6%, about 0.7%, about 0.8%, about 0.9%, about 1.0%, about 1.1%, about 1.2%, about 1.3%, about 1.4%, about 1.5%, about 1.6%, and any range therebetween, by weight of the composition, of a pH adjuster selected from the group consisting of potassium hydroxide, sodium hydroxide, ammonium hydroxide, aminomethylpropanol, triethanolamine, tetrahydroxypropylethylenediamine, and any combination thereof.

[0088] When a polymeric emulsifier and a pH adjuster are used, it may be desirable to optimize the ratio of polymeric emulsifier to pH adjuster. For example, the weight ratio of polymeric emulsifier to pH adjuster can be between about 1:5 and about 1:0.5, or between about 1:3 and about 1:1, such as about 1:3, about 1:2.5, about 1:2, about 1:1.5, about 1:1.1, and any range therebetween.

[0089] thickener The composition may further comprise a thickening agent (also called a viscosifying agent), or an additional thickening agent if the emulsifier in the composition also functions as a thickening agent. The composition may comprise from about 0.1% to about 5%, or alternatively from about 0.2% to about 2%, of the thickening agent, or additional thickening agent, if present. Suitable classes of thickening agents include, but are not limited to, carboxylic acid polymers, polyacrylamide polymers, sulfonated polymers, copolymers thereof, hydrophobically modified derivatives thereof, and mixtures thereof.

[0090] The thickening agent may be an acrylate crosslinked silicone copolymer network (sometimes referred to as a "polyacrylate siloxane copolymer network"). Suitable thickening agents may also generally include carboxylic acid polymers, polyacrylamide polymers or copolymers, sulfonated polymers, gums, clays, cellulose or modified cellulose compositions, and the like.

[0091] Other ingredients In addition to the above ingredients, the compositions herein may further include preservatives and preservative enhancers, for example, water-soluble or solubilizable preservatives such as Germall 115, methyl, ethyl, propyl, and butyl esters of hydroxybenzoic acid, benzyl alcohol, imidazolidinyl urea, EDTA and its salts, bronopol (2-bromo-2-nitropropane-1,3-diol), and phenoxypropanol; antifoaming agents; binders; biological additives; bulking agents; colorants; essential oils and their solubilizers; other natural extracts; compounds that stimulate collagen production; yeast fermentation filtrate, and the like.

[0092] Skin activators The compositions of the present invention may contain skin active agents that provide specific skin care benefits characteristic of skin care product use. Herein, skin care benefits may include benefits related to skin appearance or makeup. Skin care actives can provide acute (immediate and short-term) benefits or chronic (long-term and long-lasting) benefits.

[0093] Skin active agents useful herein include skin lightening agents, anti-acne agents, emollients, nonsteroidal anti-inflammatory agents, topical anesthetics, artificial tanning agents, antimicrobial and antifungal actives, skin soothing agents, sunscreening agents, such as lotions or creams with SPF, skin barrier repair agents, anti-wrinkle agents, anti-atrophy actives, lipids, sebum inhibitors, skin sensates, protease inhibitors, anti-itch agents, hair growth inhibitors, desquamation enzyme enhancers, anti-glycation agents, and mixtures thereof. When included, the compositions comprise from about 0.001% to about 20%, preferably from about 0.1% to about 10%, of at least one skin active agent.

[0094] The type and amount of skin active agent is selected so that the inclusion of a particular agent does not affect the stability of the composition. For example, a hydrophilic agent may be incorporated in an amount soluble in the aqueous phase, while a lipophilic agent may be incorporated in an amount soluble in the oil phase.

[0095] Other skin active agents purported to exhibit expression wrinkle reducing benefits for use in the present invention include, but are not limited to, Lavandox available from Barnet Products Corporation, Thallasine 2 available from BiotechMarine, Argireline NP available from Lipotec, Gatuline In-Tense and Gatuline Expression available from Gattefosse, Myoxinol LS 9736 from BASF Chemical Company, Syn-ake available from DSM Nutritional Products, Inc., and Instensyl® available from Silab, Inc., and Sesaflash™ available from Seppic Inc.

[0096] Skin lightening agent useful herein refers to the active ingredient that improves hyperpigmentation compared with before treatment.Skin lightening agent useful herein includes ascorbic acid compound, vitamin B3 compound, azelaic acid, butylhydroxyanisole, gallic acid and its derivatives, glycyrrhizic acid, hydroquinone, kojic acid, arbutin, mulberry extract, and their mixtures.The use of a combination of skin lightening agents is considered to be advantageous because they can provide skin lightening benefits through different mechanisms.

[0097] Ascorbic acid compounds useful herein include ascorbic acid itself in the L-form, ascorbate salts, and their derivatives. Ascorbate salts useful herein include sodium, potassium, lithium, calcium, magnesium, barium, ammonium, and protamine salts. Ascorbic acid derivatives useful herein include, for example, ascorbic acid esters and ascorbic acid ester salts. Particularly preferred ascorbic acid compounds include 2-OD-glucopyranosyl-L-ascorbic acid, which is an ester of ascorbic acid and glucose and is commonly referred to as L-ascorbic acid 2-glucoside or ascorbyl glucoside, and its metal salts, as well as L-ascorbic acid phosphate salts, such as sodium ascorbyl phosphate, potassium ascorbyl phosphate, magnesium ascorbyl phosphate, and calcium ascorbyl phosphate. Commercially available ascorbic acid compounds include magnesium ascorbyl phosphate available from Showa Denko, 2-OD-glucopyranosyl-L-ascorbic acid available from Hayashibara, and sodium L-ascorbyl phosphate having the trade name STAY C available from Roche.

[0098] Vitamin B3 compounds useful herein include, for example, those having the formula:

[0099] [ka] (wherein R is -CONH (e.g., niacinamide) or -CHOH (e.g., nicotinyl alcohol)), derivatives thereof, and salts thereof. Exemplary derivatives of the aforementioned vitamin B3 compounds include nicotinic acid esters, such as non-vasodilatory esters of nicotinic acid, nicotinyl amino acids, nicotinyl alcohol esters of carboxylic acids, nicotinic acid N-oxide, and niacinamide N-oxide. Preferred vitamin B3 compounds are niacinamide and tocopherol nicotinate, more preferably niacinamide. In a preferred embodiment, the vitamin B3 compound contains a limited amount of salt forms, and more preferably is substantially free of salts of the vitamin B3 compound. Preferably, the vitamin B3 compound contains less than about 50% of such salts, and more preferably is essentially free of salt forms. A commercially available vitamin B3 compound that is highly useful herein includes niacinamide USP, available from Reilly.

[0100] Other hydrophobic skin lightening agents useful herein include ascorbic acid derivatives such as ascorbyl tetrahexyldecanoate (e.g., VC-IP available from Nikko Chemical), ascorbyl palmitate (e.g., available from Roche Vitamins), ascorbyl dipalmitate (e.g., NIKKOL CP available from Nikko Chemical), undecylenoyl phenylalanine (e.g., SEPIWHITE MSH available from Seppic), octadecenedioic acid (e.g., ARLATONE DIOIC DCA available from Uniquema), oenothera biennis seed extract, and pyrus malus (apple) fruit extract, water and Myritol 318 and butylene glycol and tocopherol and ascorbyl tetrahexyldecanoate and parabens and Carbopol 980 and DNA / SMARTVECTOR available from COLETICA. UV, magnesium ascorbyl phosphate in hyaluronic filling spheres available from COLETICA, as well as mixtures thereof.

[0101] Other skin active agents useful herein include N-acetyl-D-glucosamine, panthenol (e.g., DL panthenol available from Alps Pharmaceutical Inc.), tocopheryl nicotinate, benzoyl peroxide, 3-hydroxybenzoic acid, flavonoids (e.g., flavanones, chalcones), farnesol, phytantriol, glycolic acid, lactic acid, 4-hydroxybenzoic acid, acetylsalicylic acid, 2-hydroxybutanoic acid, 2-hydroxypentanoic acid, 2-hydroxyhexanoic acid, cis-retinoic acid, trans-retinoic acid, retinol, retinyl esters (e.g., retinyl propionate), phytic acid, N-acetyl-L-cysteine, lipoic acid, tocopherol and its esters (e.g., tocopheryl acetate, DL-α-tocopheryl acetate (available from Eisai), azelaic acid, arachidonic acid, tetracycline, ibuprofen, naproxen, ketoprofen, hydrocortisone, acetaminophen, resorcinol, phenoxyethanol, phenoxypropanol, phenoxyisopropanol, 2,4,4'-trichloro-2'-hydroxydiphenyl ether, 3,4,4'-trichlorocarbanilide, octopirox, lidocaine hydrochloride, clotrimazole, miconazole, ketoconazole, neomycin sulfate, theophylline, and mixtures thereof. In preferred examples, the skin active agent is present at a level of about 0.001% to about 20%, more preferably about 0.1% to about 10%.

[0102] Use of the cosmetic composition Various methods of treatment, application, regulation or improvement can utilize the aforementioned composition.The application of this composition can be carried out on any skin surface of the body.The skin surface that is most concerned tends to be the surface that is not typically covered by clothing, such as the skin surface of the face, the skin surface of the hands and arms, the skin surface of the feet and legs, and the skin surface of the neck and chest (for example, décolleté).In particular, application can be on the skin surface of the face, such as the skin surface of the forehead, around the mouth, chin, around the orbit, nose, and / or cheek.

[0103] There are many regimens for applying the composition to the skin. The composition may be applied at least once a day, twice a day, or more frequently, daily, during the treatment period. If applied twice a day, the first and second applications are separated by at least 1 to about 12 hours. Typically, the composition may be applied in the morning and / or evening.

[0104] The cosmetic composition may be applied as a treatment regimen including other products or formulations. A secondary application of a different composition or different conditions, such as temperature, device, or ingredient-based penetration enhancers, may be applied before or after application of the composition. Preferably, such application may be incorporated as a second, third, fourth, or additional treatment step. Such application may condition the skin prior to treatment application using the cosmetic composition. Any number of products or treatment steps may be included as part of the regimen, in any required order. Examples of the use of a second or different composition include altering pH, ionic strength, temperature, adding or removing chemical or biochemical triggers prior to treatment, etc.

[0105] The step of applying the composition to the skin may be performed by localized application to the area. With respect to applying the composition, the terms "localized," "topical," or "topically" mean that the composition is delivered to the target area (e.g., an area of ​​skin containing wrinkles) while minimizing delivery to the skin surface or subcutaneous layers not requiring treatment. The composition may be applied to the skin and lightly massaged. It is recognized that localized application allows a moderate amount of the composition to be applied to an area adjacent to the target area to be treated (i.e., the composition is unlikely to be applied or remain within the boundaries of the wrinkle without some spreading). The form of the composition or acceptable carrier should be selected to facilitate localized application. While certain embodiments of the present invention contemplate applying the composition locally to the target area, it is understood that the compositions of the present invention may be applied more generally or broadly to one or more facial skin surfaces to reduce the appearance of wrinkles in facial skin areas. Similarly, the composition may be applied as a continuous thin film or in a pattern. Streaks, patterned patches, or random application of the composition may be desired. As described below, an applicator can be useful to assist in patterned deposition.

[0106] According to a specific method, the composition can be applied to the skin area where desired effect is desired.For example, the composition can be applied to the hairline, temples, jawline, and other peripheral areas of the face to apply effect to other facial areas.This method utilizes the effect on the peripheral areas of the face to, for example, reduce the appearance of wrinkles in the eye area, smile lines around the mouth, wrinkles under the eyes, and smooth wrinkles around the cheek area.According to this method, the composition can be applied to the peripheral areas of the face without directly applying the composition to the target area.

[0107] In another aspect, the present disclosure provides a method for improving mammalian skin, comprising administering an effective amount of a composition. In some embodiments, improving the mammalian skin comprises treating a keratinous tissue condition in the mammal. Such treatment of the keratinous tissue condition may comprise topical application and may include improving the cosmetic appearance of the mammalian keratinous tissue. In some embodiments, the methods include, but are not limited to, preventing, delaying, and / or treating uneven skin tone, reducing the size of pores in mammalian skin, regulating the oily / shiny appearance of mammalian skin, thickening the stratum corneum (i.e., building up the stratum corneum of the epidermal and / or dermal and / or subcutaneous tissue layers of the skin, and the nails and hair shafts, where applicable), preventing, delaying, and / or treating uneven skin tone by acting as a lightening agent or hypopigmentation cosmetic agent, preventing, delaying, and / or treating atrophy of mammalian skin, softening and / or smoothing mammalian lips, hair, and nails, preventing, delaying, and / or treating itchy mammalian skin, preventing, delaying, and / or treating the appearance of dark under-eye circles and / or puffy eyes, and / or In some embodiments, the compositions are used to treat signs of aging, including preventing, delaying, and / or treating pale skin in mammals, preventing, delaying, and / or treating sagging skin (i.e., glycation) in mammals, preventing and / or delaying sunburn in mammalian skin, desquamating and peeling mammalian skin and / or increasing skin turnover in mammalian skin, preventing, delaying, and / or treating hyperpigmentation such as post-inflammatory hyperpigmentation, preventing, delaying, and / or treating the appearance of spider veins and / or red spots in mammalian skin, preventing, delaying, and / or treating fine lines and wrinkles in mammalian skin, preventing, delaying, and / or treating dry skin (i.e., roughness, scaling, peeling), and preventing, delaying, and / or treating the appearance of cellulite in mammalian skin. In some embodiments, the compositions are used to treat signs of aging. For example, in some embodiments, the compositions are used to regulate the signs of aging.In some embodiments, the compositions are used to reduce or diminish the signs of aging. In some embodiments, the compositions are used to prevent the signs of aging in keratinous tissue (e.g., skin, hair, or nails). Improving the condition of keratinous tissue can include topically applying a safe and effective amount of a composition of the present disclosure to keratinous tissue.

[0108] Non-limiting examples of skin care compositions include, but are not limited to, sunscreens and sunblocks, mousses, bath and shower gels, lip balms, skin conditioners, cold creams, moisturizers, soaps, body scrubs, body washes, face washes, body sprays, exfoliants, astringents, scrubbing lotions, depilatories, shaving, pre-shave and after-shave products, deodorants and antiperspirants, cleansers, skin gels and rinses, skin lightening and self-tanning compositions. Non-limiting examples of hair care compositions include, but are not limited to, shampoos, conditioners, treatments, styling products, hair sprays, permanent styling products, tonics, cream rinses, hair dyes, hair coloring products, hair bleaching products, hair polishes, hair serums, anti-frizz products, volumizers, split end repair products, anti-dandruff formulations, and mascara. Non-limiting examples of other cosmetic compositions include, but are not limited to, makeup, such as lipstick, rouge, foundation, blush, eyeliner, lip liner, lip gloss, facial or body powder, nail polish, and eye shadow, among others. Additionally, the compositions may be applied topically through the use of a patch or other delivery device. Delivery devices may include, but are not limited to, those that can be heated or cooled, as well as those that utilize iontophoresis or ultrasound. In some embodiments, for example, the compositions described herein are in the form of a skin lotion, clear lotion, milky lotion, cream, gel, foam, ointment, paste, emulsion, spray, conditioner, tonic, makeup, lipstick, foundation, nail polish, aftershave, or the like that is intended to be left on the skin or other keratinous tissue for some aesthetic, preventative, therapeutic, or other benefit (i.e., a "leave-on" or skin care composition).After applying the composition to the keratinous tissue (e.g., skin), it is preferably left on for at least about 2 minutes, 5 minutes, 15 minutes, more preferably at least about 30 minutes, even more preferably at least about 1 hour, and even more preferably at least several hours, for example, up to about 12 hours. Any part of the outer parts of the face, hair, and / or nails can be treated (e.g., face, lips, under-eye area, eyelids, scalp, neck, torso, arms, hands, legs, feet, fingernails, toenails, scalp hair, eyelashes, eyebrows, etc.). The composition may be applied using the palm and / or fingers, or a device or implement (e.g., cotton ball, cotton swab, pad, applicator pen, spray applicator, etc.).

[0109] Another approach to ensuring continuous exposure of the keratinous tissue to at least a minimum level of the composition is to apply the compound, for example, by using a patch applied to the face. Such an approach is particularly useful for problem skin areas requiring more intensive treatment (e.g., facial crow's feet, frown lines, under-eye area, upper lip, etc.). The patch may be occlusive, semi-occlusive, or non-occlusive, and may be adhesive or non-adhesive. The composition may be contained within the patch or may be applied to the skin before application of the patch. The patch may also contain additional active substances, such as chemical initiators for exothermic reactions. The patch may also contain an electrical energy source (e.g., a battery) to increase delivery of the composition and active agent (e.g., iontophoresis). The patch is preferably left on the keratinous tissue for a period of at least about 5 minutes, or at least about 15 minutes, or at least about 30 minutes, or at least about 1 hour, or overnight as a form of overnight therapy.

[0110] Applicator In some embodiments, the composition can be delivered by a variety of applicators suitable for localized and systemic application. For example, suitable applicators may be droppers and bottles containing the composition. Pen-like wands with housings that can contain the composition can also be used. The wands may include a handle, a stem, and an applicator head. The applicator head may include fiber, foam, cotton, a rollerball, or any other suitable material capable of releasably holding the composition. Exemplary applicators include devices, penetration enhancers, and microneedles, among others.

[0111] A simple cotton swab can apply the composition topically to wrinkled areas. The applicator is configured to easily apply the composition to wrinkled areas having an approximate diameter of between about 2 mm and about 20 mm, and provides a dose of about 0.01 to about 2 mg / cm. 2 or between about 0.1 and about 1 mg / cm 2 The thickness of the applied film can be measured or calculated based on the application area and the applied dose given immediately above.

[0112] In another embodiment, the applicator may be in the form of a pre-treated tape. The tape may be treated or impregnated with the compositions herein and then applied to the skin via any suitable tape dispensing mechanism. Thus, the applicator may take the form of a transdermal patch, a microneedle applicator, etc.

[0113] kit As described above and generally herein, the present invention also provides kits containing the compositions. The kits are typically provided in a suitable container (e.g., foil, plastic, or cardboard packaging). The kits of the present invention may also include one or more excipients, additives, etc., as described herein. The kits of the present invention may also include means for proper administration, including an applicator. The kits of the present invention may also include instructions for proper administration and / or preparation for proper administration.

[0114] Although some methods described herein contemplate applying the compositions of the present invention with an applicator, it is understood that an applicator is not required and the compositions of the present invention can also be applied directly by using a finger or in other conventional manner.

[0115] The dimensions and values ​​disclosed herein should not be understood as being strictly limited to the exact numerical values ​​recited. Instead, unless otherwise specified, each such dimension is intended to mean both the recited value and a functionally equivalent range surrounding that value. [Example]

[0116] Example 1: Process for Producing Aloe Fermentate with Structured Water in the Fermentation Mixture (Sample 1) The process under controlled, optimal conditions is described below. A fermentation mixture was prepared by mixing 1% Aloe barbadensis leaf powder, quenched structured water (I / S), and 0.1% Lactobacillus sp. Fermentation was carried out at temperatures below 25°C for three days to allow the microorganisms to grow under optimal conditions. On the fourth day, the temperature was increased to 45°C, and conditions were controlled for 24 hours. On the fifth day, the material was sterilized and filtered. Depending on the batch, the fermentation yield (final ferment filtrate relative to the initial fermentation mixture, w / w) could be 95% to 99%. Then, 0.03% (w / w) Aloe barbadensis leaf polysaccharide in structured water was added to restore the ferment filtrate to the weight of the initial fermentation mixture. Therefore, depending on the batch, a solution of Aloe barbadensis leaf polysaccharide (0.03% w / w) in structured water was added to the ferment filtrate in an amount ranging from approximately 1% to approximately 5% of the initial fermentation mixture. When multiple batches were combined, the combined addition of Aloe barbadensis leaf polysaccharides (0.03% w / w) solution in structured water was approximately 3% (w / w) of the combined weight of the initial fermentation mixture. The filtration step involves an initial filtration to remove large particles from the fermentate, followed by multiple filter steps with progressively smaller pore sizes to achieve the appropriate pore size. The first filter is a D / E filter. The material is then passed through a continuous filtration system of 1.0 micron, 0.45 micron, and then 0.2 micron filters. The material is then stored and finally passed through a 0.22 micron sterilizing filter. The filtration step generally takes anywhere from several days to several weeks, depending on the bulk material.

[0117] Example 2: Comparison Samples (Samples 2, 3, and 4) An aloe fermentate (Sample 2) without structured water in the fermentation mixture was prepared as follows: The fermentation mixture was prepared by combining 1% Aloe barbadensis leaf powder, quenched syrup water, and 0.1% Lactobacillus sp. Fermentation was carried out at temperatures below 25°C for three days to allow the microorganisms to grow under optimal conditions. On day four, the temperature was increased to 45°C and conditions were controlled for 24 hours. On day five, the material was sterilized and filtered. Depending on the batch, the fermentation yield (final ferment filtrate relative to the initial fermentation mixture, w / w) could be 95% to 99%. Then, 0.03% (w / w) Aloe barbadensis leaf polysaccharide in structured water was added to the ferment filtrate to restore its weight to the initial fermentation mixture. Therefore, depending on the batch, a solution of Aloe barbadensis leaf polysaccharide (0.03% w / w) in structured water was added to the ferment filtrate in an amount ranging from approximately 1% to approximately 5% of the initial fermentation mixture. When multiple batches were combined, the combined addition of Aloe barbadensis leaf polysaccharides (0.03% w / w) solution in structured water was approximately 3% (w / w) of the combined weight of the initial fermentation mixture. The filtration step involves an initial filtration to remove large particles from the fermentate, followed by multiple filter steps with progressively smaller pore sizes to achieve the appropriate pore size. The first filter is a D / E filter. The material is then passed through a continuous filtration system of 1.0 micron, 0.45 micron, and then 0.2 micron filters. The material is then stored and finally passed through a 0.22 micron sterilizing filter. The filtration step generally takes anywhere from several days to several weeks, depending on the bulk material.

[0118] A Lactobacillus fermentate (Sample 3) without structured water or aloe in the fermentation mixture was prepared as follows: The fermentation mixture was prepared by combining 1% glucose, QS water, and 0.1% Lactobacillus SP. Fermentation was carried out at temperatures below 25°C for three days to allow the microorganisms to grow under optimal conditions. On day four, the temperature was increased to 45°C and conditions were controlled for 24 hours. On day five, the material was sterilized and filtered. The filtration step involves an initial filtration to remove a large amount of large particles from the fermentate, followed by multiple filter steps in which the fermentate achieves the appropriate pore size with progressively smaller pore sizes. The first filter is a D / E filter. The material is then passed through a continuous filtration system with 1.0 micron, 0.45 micron, and then 0.2 micron filters. The material is then stored and finally passed through a 0.22 micron sterilizing filter. The filtration step generally takes anywhere from several days to several weeks, depending on the bulk material.

[0119] Aloe barbadensis leaf polysaccharide (Sample 4) was purchased from Ashland under the trade name ALOE VERA IL SD PWD 200X 705AV ORGANIC.

[0120] Example 3: Comparison of Efficacy Studies (Samples 1, 2, 3, and 4) Each of Samples 1, 2, 3, and 4 was prepared as a 2% solution in purified water and mixed by vortexing and / or sonication, if necessary. Each sample was assayed using a modified protocol of the Randox TAS kit.

[0121] In a 96-well plate, reagents and samples were added to each well to be assayed (n=3). Due to instrument limitations, a maximum of 36 wells can be used. To determine antioxidant capacity, absorbance (600 nm) was measured over a 2-minute period on a Spectramax Plate Reader. Percentages were calculated, followed by inhibition of oxidation for each sample compared to a water blank. BHT was tested at 0.001% as a control, which is within the expected range.

[0122] [Table 1]

[0123] Therefore, the experimental results in Table 1 clearly showed that Sample 1 (using structured water as the fermentation medium) was significantly better at inhibiting oxidation than Samples 2 (which had nearly the same production process as Sample 1, except that Sample 2 did not use structured water in the fermentation medium), 3, and 4 when tested at the same concentrations in this assay.

[0124] Example 4: Stimulatory activity of aloe fermentate on adult normal human dermal fibroblasts (NHDF) Aloe fermentate (Sample 1) was tested for collagen-enhancing activity in an ELISA assay. Aloe fermentate was found to increase collagen synthesis in a dose-dependent manner. Collagen synthesis increased by 16%, 21%, and 48% at 0.25%, 0.5%, and 1% (v / v), respectively. 20 ng / ml of TGFβ was found to increase collagen synthesis by 91% in the assay.

[0125] Adult NHDF cells (Clonetics) were seeded into 96-well plates (Corning / Costar flat-bottom) and grown to confluence before treatment. Samples were tested with aloe ferment at 0.125%, 0.25%, 0.5%, and 1% (v / v). Plates were incubated at 37°C / 5% CO2 for 3 days, after which the supernatants were collected and stored at -80°C in siliconized tubes until ELISA was performed. PIP ELISA was performed, and results were calculated from a standard curve. For procollagen ELISA, supernatants were diluted 1:200. TGFβ (20 ng / ml) was run as a positive control in the assay. Cell viability was determined by a standard MTT assay. Statistical significance was determined using one-way ANOVA followed by Dunnett's post-hoc test. All results have a p<0.01. All analyses were performed using GraphPad Instat.

[0126] [Table 2]

[0127] Table 2 shows the results of aloe ferment when tested at 0.125%, 0.25%, 0.5%, and 1% (v / v) for collagen-enhancing activity. Collagen synthesis was significantly altered by -9%, +16%, +21%, and +48%, respectively, while cell viability was not significantly affected. The results conclude that aloe ferment enhanced collagen synthesis in a dose-dependent manner at 0.25%, 0.5%, and 1%.

[0128] Example 5: Clinical moisturizing research Two clinical studies were conducted to investigate the effects of a composition containing the inventive aloe ferment (Sample 1) described herein, particularly the moisturizing effect of the composition on human skin.

[0129] Study participants (N=16 and N=110) were enrolled in each study. Study participants included all age groups between 19 and 65 years old, and all skin types, including dry, oily, and combination skin. Participants applied the composition to cleansed skin both morning and evening during the day. The composition was massaged topically onto cleansed skin, and the study protocol did not include the introduction of other skin care products during the study period. The moisturizing effect on participants' skin was measured. All participants (100%) showed immediate improvement and moisturization upon topical application of the composition. Approximately 89% of participants showed improved moisturization 72 hours after topical application. Approximately 68% of participants showed improved moisturization 100 hours after topical application.

[0130] Example 6 In vitro study of the effects of aloe ferment on dark skin An in vitro study was conducted to evaluate the effects of the aloe ferment according to the invention described herein (Sample 1) on dark skin, particularly the skin lightening and skin brightening effects.

[0131] The aloe ferment / extract was diluted to 1% (v / v) in DPBS and then further diluted to 0.5%-0.125% in DPBS. Solutions were prepared prior to study treatment. Study samples, i.e., tissues, were treated daily (except weekends) for 14 days with 3 μl of diluted aloe ferment. The volume of solution was pipetted and applied with the tip of a sterile glass rod, leaving 2 μl on the insert. Before application, the inserts were washed twice with DPBS. Tissue viability and melanin content were measured 7 and 14 days after treatment.

[0132] Melanin Analysis: Tissues were removed from the plastic inserts and placed in 250 μl of Solvable in a 0.6 mL microfuge tube. Tissues were incubated overnight at 60°C. Samples were vortexed and then centrifuged at 13,000 rpm for 5 minutes. 200 μl of sample was pipetted into a 96-well plate and read at 490 nm in a spectrophotometer and measured against melanin standards.

[0133] [Table 3]

[0134] Viability analysis: A 10% Alamar Blue solution was made by combining 13 mL of Alamar Blue and 117 mL of maintenance medium. The maintenance medium was removed from each well and replaced with 5 mL of 10% Alamar Blue in each well. The inserts were incubated in 10% Alamar Blue for 2 hours. After removal of the inserts, the fluorescence of each well was measured using a Spectra Max M2 e Measurements were taken on a plate reader at 530 nm excitation and 590 nm emission.

[0135] [Table 4]

[0136] The study results, shown in Tables 3 and 4, indicate that at 1 week, 1% aloe ferment was effective in reducing normalized melanin by 14% while increasing tissue viability by 6%. At 2 weeks, 1% aloe ferment was effective in reducing melanin by 22% while increasing viability by 23%. Additionally, 0.25% and 0.5% aloe ferment were also effective in reducing melanin by 18% and 19%, respectively.

[0137] All documents cited herein, including any cross-referenced or related patents or applications, are incorporated herein by reference in their entirety unless expressly excluded or otherwise limited. The citation of any document is not an admission that it is prior art with respect to any invention disclosed or claimed herein, or that it alone, or in any combination with any other reference or references, teaches, suggests, or discloses any such invention. Furthermore, in the event that any meaning or definition of a term in this document conflicts with any meaning or definition of the same term in a document incorporated by reference, the meaning or definition assigned to that term in this document shall control.

[0138] While particular embodiments of the present invention have been illustrated and described, it would be obvious to those skilled in the art that various other changes and modifications can be made without departing from the scope of the invention. It is therefore intended to cover in the appended claims all such changes and modifications that are within the scope of this invention. The present invention includes, for example, the following embodiments. [Section 1] A cosmetic composition comprising a fermentate from a fermentation mixture comprising at least one prebiotic cosmetic ingredient, a structured water component, and at least one probiotic microorganism. [Section 2] Item 1. The cosmetic composition according to item 1, wherein the ferment further comprises at least one cosmetic active ingredient. [Section 3] Item 1. The cosmetic composition according to item 1, wherein the structural water component comprises I water and S water. [Section 4] Item 4. The cosmetic composition according to Item 3, wherein the water is present in an amount of about 1% to about 60% by weight of the fermentate. [Section 5] Item 4. The cosmetic composition according to Item 3, wherein the S water is present in an amount of about 1% to about 60% by weight of the fermentate. [Section 6] Item 1. The cosmetic composition according to item 1, wherein the at least one prebiotic active ingredient comprises aloe, aronia berry, raspberry, spinach, cherry broccoli, tart cherry, apple, mushroom, grape juice, beet, blueberry, blackberry, acai, coffee, ginger, cranberry, raspberry, aloe gel, coconut water, pomegranate, mango, apricot, retinol, raspberry, lavender, honey, cardamom, truffle, hyaluronic acid, or a mixture thereof. [Section 7] Item 2. The cosmetic composition according to item 1, wherein the at least one probiotic microorganism is a strain selected from the group consisting of Bifidobacterium, Lactobacillus, Enterococcus, Streptococcus, Staphylococcus, Streptococcus, Saccharomyces cerevisiae, Saccharomyces boulardii, and mixtures thereof. [Section 8] Item 1. The cosmetic composition according to item 1, wherein the fermentate is in the form of an extract, a lysate, or a filtrate. [Section 9] Item 2. The cosmetic composition according to Item 1, wherein the fermentate is present in an amount of about 0.00001% by weight to about 20% by weight based on the total weight of the cosmetic composition. [Section 10] Item 2. The cosmetic composition according to Item 1, wherein the structural water component is present in the fermentate in an amount of about 1% by weight to about 99.5% by weight, based on the total weight of the fermentate. [Section 11] Item 7. The cosmetic composition according to item 6, wherein the at least one prebiotic active ingredient is Aloe barbadensis leaf powder. [Section 12] Item 7. The cosmetic composition according to item 6, wherein the at least one prebiotic active ingredient is present in an amount ranging from about 0.001% by weight to about 20% by weight, based on the total weight of the fermentation mixture. [Section 13] Item 1. The cosmetic composition according to Item 1, wherein the composition is a skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nourishing lotion, massage cream, facial mask, facial cleanser, nourishing cream, moisturizing cream, hand cream, foundation, primer, essence, nourishing essence, cleansing foam, cleansing lotion, cleansing cream, body lotion, cream with SPF, lotion with SPF, body mask, or body cleanser. [Section 14] A method for increasing collagen production in human skin, comprising the step of applying a cosmetic composition containing a fermentate from a fermentation mixture comprising at least one prebiotic active ingredient, a structured water component, and at least one probiotic microorganism. [Section 15] Item 15. The method according to item 14, wherein the cosmetic composition is applied once, twice or three times a day, or at night before going to bed. [Section 16] Item 15. The method of item 14, wherein the cosmetic composition enhances skin hydration and moisturization for up to 100 hours upon topical application. [Section 17] Item 15. The method of item 14, wherein the composition provides skin brightening and skin lightening benefits to the skin upon topical application.

Claims

1. A cosmetic composition comprising a ferment, The fermented body is a structured aqueous solution or suspension comprising at least one cosmetic active ingredient comprising Aloe barbadensis leaf polysaccharides and a first structured water component; and Contains fermentation products, The fermentation product is at least one probiotic microorganism comprising a strain of the genus Lactobacillus; at least one prebiotic active ingredient comprising Aloe barbadensis leaf powder; and Second structural water component Produced by fermentation of a fermentation mixture containing The cosmetic composition, wherein the fermentation product is a filtrate.

2. 10. The cosmetic composition of claim 1, wherein the at least one cosmetic active ingredient further comprises hyaluronic acid.

3. A cosmetic composition as described in claim 1, wherein the first and second structural water components comprise I water and S water.

4. 4. The cosmetic composition of claim 3, wherein the ferment comprises water in an amount of 1% to 60% by weight of the ferment.

5. 4. The cosmetic composition of claim 3, wherein the ferment comprises S water in an amount of 1% to 60% by weight of the ferment.

6. 10. The cosmetic composition of claim 1, wherein the at least one prebiotic active ingredient further comprises aronia berry, spinach, cherry broccoli, tart cherry, apple, mushroom, grape juice, beet, blueberry, blackberry, acai, coffee, ginger, cranberry, aloe gel, coconut water, pomegranate, mango, apricot, retinol, raspberry, lavender, honey, cardamom, truffle, hyaluronic acid, or mixtures thereof.

7. The strain of the genus Lactobacillus is selected from the group consisting of Lactobacillus johnsonii, Lactobacillus paracasei, Lactobacillus acidophilus, Lactobacillus amylovorus, Lactobacillus casei, Lactobacillus rhamnosus, Lactobacillus brevis, Lactobacillus crispatus, Lactobacillus delbrueckii, and Lactobacillus fermentum.

4. The cosmetic composition of claim 1, wherein the active ingredient is selected from the group consisting of Lactobacillus fermentum, Lactobacillus helveticus, Lactobacillus gallinarum, Lactobacillus gasseri, Lactobacillus plantarum, Lactobacillus reuteri, Lactobacillus salivarius, Lactobacillus alimentarius, Lactobacillus curvatus, Lactobacillus sake, and mixtures thereof.

8. 2. The cosmetic composition according to claim 1, wherein the cosmetic composition comprises the ferment in an amount of 0.00001% to 20% by weight, relative to the total weight of the cosmetic composition.

9. 10. The cosmetic composition of claim 1, wherein the ferment comprises the first and second structured water components in an amount of 1% to 99.5% by weight, combined, based on the total weight of the ferment.

10. The cosmetic composition of claim 6 , wherein the at least one prebiotic active ingredient further comprises hyaluronic acid.

11. 2. The cosmetic composition according to claim 1, wherein the fermented mixture comprises at least one prebiotic active ingredient in an amount ranging from 0.001% to 20% by weight, relative to the total weight of the fermented mixture.

12. 10. The cosmetic composition of claim 1, wherein the composition is a skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nourishing lotion, massage cream, facial mask, facial cleanser, nourishing cream, moisturizing cream, hand cream, foundation, primer, essence, nourishing essence, cleansing foam, cleansing lotion, cleansing cream, body lotion, cream with SPF, lotion with SPF, body mask, or body cleanser.

13. 1. A method for increasing collagen production in human skin, comprising: applying a cosmetic composition comprising the ferment, The fermented body is a structured aqueous solution or suspension comprising at least one cosmetic active ingredient comprising Aloe barbadensis leaf polysaccharides and a first structured water component; and fermentation products Contains The fermentation product is at least one probiotic microorganism comprising a strain of the genus Lactobacillus; at least one prebiotic active ingredient comprising Aloe barbadensis leaf powder; and Second structural water component Produced by fermentation of a fermentation mixture containing The method wherein the fermentation product is a sterile filtrate.

14. 14. The method of claim 13, wherein the cosmetic composition is applied once, twice, or three times daily, or at night before going to bed.

15. 14. The method of claim 13, wherein the cosmetic composition enhances skin hydration and moisturization for up to 100 hours upon topical application.

16. 14. The method of claim 13, wherein the cosmetic composition provides skin brightening and skin lightening benefits to the skin upon topical application.

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