Glucagon-like peptide-1 (GLP-1) agonist analogs, processes for their preparation and uses - Patent Application 20070122997

Abstract

The present disclosure relates to analogs of glucagon-like peptide-1 (glp-1) receptor agonists. The present disclosure provides analogs of glucagon-like peptide-1 (glp-1) receptor agonists, in which the amino acid at position 2 of the glucagon-like peptide-1 (glp-1) receptor agonist is replaced with D-alanine. The glucagon-like peptide-1 (glp-1) analogs have one or more of the following properties: an extended half-life, a better pharmacokinetic profile, retained biological activity, and the advantage of reducing the frequency and dosage of administration, thereby reducing the burden on patients. The present disclosure also provides a process for preparing synthetic glucagon-like peptide-1 (glp-1) analogs.

Description

[Technical Field]

[0001] The present disclosure relates to analogs of glucagon-like peptide-1 (glp-1). More specifically, the present disclosure relates to analogs of glucagon-like peptide-1 (glp-1) receptor agonists, in which the amino acid at position 2 of a native glucagon-like peptide-1 (glp-1) receptor agonist is replaced with D-alanine. The present invention further relates to analogs of glucagon-like peptide-1 (glp-1) that have one or more of the following properties: an extended half-life, a better pharmacokinetic profile, retained biological activity, and the advantage of reducing the frequency and dosage of administration, thereby reducing patient burden. In particular, the present invention relates to synthetic glucagon-like peptide-1 (glp-1) analogs obtained from different peptide synthesis processes, and to processes for preparing synthetic glucagon-like peptide-1 (glp-1) analogs. [Background technology]

[0002] The Background Description includes information that may be useful in understanding the present invention. This is not an admission that any information provided herein is prior art or related to the present invention, nor is it an admission that any publication mentioned, expressly or implicitly, is prior art.

[0003] As a drug class, long-acting GLP-1 receptor agonists improve glycemic control in patients with type 2 diabetes and, due to their glucose-dependent mechanism of action, have a low risk of hypoglycemia. Glucagon-like peptide-1 (GLP-1) is produced in the intestine and stimulates insulin secretion while inhibiting glucagon secretion in a glucose-dependent manner, reducing appetite and energy intake and slowing gastric emptying. This drug class has also been demonstrated to promote weight loss and lower SBP, which are beneficial for patients with type 2 diabetes and may reduce cardiovascular risk. Furthermore, although nausea is a common side effect associated with long-acting GLP-1 receptor agonists, it is often transient, and overall, long-acting GLP-1 receptor agonists are generally well tolerated. Therefore, long-acting GLP-1 receptor agonists may be an effective treatment option for individuals with type 2 diabetes and are well positioned to meet the standard of care guidelines set by the American Diabetes Association (ADA) for treatment that goes beyond glycemic control alone. GLP-1 is susceptible to cleavage at position 2 (alanine) by the ubiquitous dipeptidyl peptidase (DPP)-4, which occurs almost immediately upon secretion, resulting in a short half-life of GLP-1 of <2 minutes (Gupta V., Indian J Endocr Metab 2013, 17, 413-21).

[0004] Many GLP-1 agonists have been developed by modifying native GLP-1 to overcome the short half-life issue. One approach used was to replace one or more amino acids in the GLP-1 polypeptide to add lipophilic substituents to the peptide. These lipophilic substituted GLP-1 agonists showed prolonged action when injected. US6268343 discloses such fatty acid acylated GLP-1 agonists. One specific example of a GLP-1 analog is liraglutide, an acylated glucagon-like peptide-1 (GLP-1) agonist derived from human GLP-1-(7-37), a relatively minor form of endogenous GLP-1. Liraglutide has a short plasma half-life (9-15 hours), and novel strategies have been developed to increase its half-life and thereby exploit its antihyperglycemic effects. Treatment requires a once-daily injection in diabetic patients.

[0005] Semaglutide is another GLP-1 analogue recently registered for the treatment of type 2 diabetes. It has two amino acid substitutions (Aib(8) and Arg(34)) compared to human GLP-1 and is derivatized at lysine 26.

[0006] Several studies have been conducted to evaluate the pharmacokinetics of semaglutide when administered subcutaneously once weekly. With a 7-day half-life at doses of 0.5 mg or 1 mg, semaglutide would likely reach steady state in 4–5 weeks. However, some drug-drug interactions exist, necessitating dose adjustment. Furthermore, like other GLP-1 RAs, semaglutide can delay gastric emptying, potentially affecting oral absorption. While semaglutide may be useful in subjects with type 2 diabetes, it has been observed to slightly increase retinopathy. Furthermore, it is unknown whether semaglutide improves cardiovascular outcomes in other populations, including those with lower HbA1c levels and similar weights than those included in failed clinical outcome trials using the GLP-1R agonists lixisenatide and exenatide. Another challenge associated with semaglutide is the dosage required for oral formulations. Oral semaglutide is much higher than Ozempic-brand semaglutide injection, which required 14 mg of semaglutide per dose to achieve the described effect in clinical trials, compared with only 0.5 mg for Ozempic, yet achieved slightly better results. This difference is the result of most of the active oral drug being digested in the stomach and small intestine, with only a small portion crossing the intestinal wall on its way to the liver to achieve its therapeutic effect. Summary of the Invention

[0007] Research so far suggests that although semaglutide can improve blood sugar control and promote weight loss, it has several drawbacks, including the need for injected medication or much higher doses of semaglutide in oral formulations, frequent side effects, an increased risk of retinopathy, and potential cost. However, analogs of GLP-1 with improved half-lives and therefore greater bioavailability while retaining clinical efficacy have not been fully explored. Therefore, there is a need to develop GLP-1 analogs that can overcome the deficiencies associated with the prior art. Thus, there remains a need to provide analogues of GLP-1 that can overcome one or more of the aforementioned drawbacks, thereby enabling promising candidates such as GLP-1 analogues such as liraglutide and semaglutide to gain their due in diabetes and other treatments.

[0008] The object of the present disclosure is to provide analogs of glucagon-like peptide-1 (glp-1) receptor agonists that may overcome one or more of the shortcomings of existing glucagon-like peptide-1 (glp-1) analogs. An object of the present disclosure is to provide analogs of glucagon-like peptide-1 (glp-1) receptor agonists, in which the amino acid at position 2 of the naturally occurring glucagon-like peptide-1 (glp-1) receptor agonist is replaced with D-alanine. An object of the present disclosure is to provide a process for preparing analogs of glucagon-like peptide-1 (glp-1) receptor agonists in which the amino acid at position 2 of the native glucagon-like peptide-1 (glp-1) receptor agonist is replaced with D-alanine. The object of the present disclosure is to provide analogs of glucagon-like peptide-1 (glp-1) that retain the biological activity of the peptide, extend the half-life, have a better pharmacokinetic profile, and can be advantageous in reducing patient burden by reducing dosing frequency and dose.

[0009] An object of the present disclosure is to provide analogues of liraglutide and semaglutide that can overcome one or more deficiencies of the existing technology. The object of the present disclosure is to provide analogues of liraglutide and semaglutide that have one or more of the following properties: an extended half-life and an improved pharmacokinetic profile, while retaining their respective specific biological activities, and are advantageous in reducing the burden on patients by reducing the dosing frequency and dose. Another object of the present invention is to provide synthetic analogues of liraglutide and semaglutide that can be easily synthesized.

[0010] In certain aspects, the present disclosure provides analogs of glucagon-like peptide-1 (glp-1) receptor agonists that may overcome one or more of the shortcomings of existing glucagon-like peptide-1 (glp-1) agonists. In one aspect, the present disclosure provides an analog of a glucagon-like peptide-1 (glp-1) receptor agonist, in which the amino acid at position 2 of the native glucagon-like peptide-1 (glp-1) receptor agonist is replaced with D-alanine. In one aspect, the present disclosure provides an analog of a glucagon-like peptide-1 (glp-1) receptor agonist, wherein the glucagon-like peptide-1 (glp-1) receptor agonist is liraglutide or semaglutide.

[0011] In one aspect, the present disclosure provides a process for preparing an analog of a glucagon-like peptide-1 (glp-1) receptor agonist in which the amino acid at position 2 of the native glucagon-like peptide-1 (glp-1) receptor agonist is replaced with D-alanine. In another aspect, the present disclosure provides analogs of liraglutide in which the L-alanine amino acid at position 2 of the natural glucagon-like peptide-1 (glp-1) receptor agonist is replaced with D-alanine. In another aspect, the disclosure provides an analogue of semaglutide in which the Aib (aminoisobutyric acid) amino acid at position 2 of the natural glucagon-like peptide-1 (glp-1) receptor agonist is replaced with D-alanine.

[0012] In another aspect, the present disclosure provides a method of lowering glucose levels in a patient in need thereof, comprising administering a liraglutide analog or a semaglutide analog of the present disclosure. In another embodiment, the present disclosure provides long-acting liraglutide analogs for weekly, biweekly, or monthly administration. In a further aspect, the present invention provides a process for preparing D-liraglutide in which the amino acid at position 2 of natural liraglutide is replaced with D-alanine, comprising the steps of: a) anchoring Fmoc-Gly-OH to a resin and capping it; b) selectively deprotecting the amino groups; c) Fragments Fmoc-Arg(Pbf)OH, Fmoc-Gly-OH, Fmoc-Arg(Pbf)OH, Fmoc-Val-OH, Fmoc-Leu-OH, Fmoc-Trp(Boc)-OH, Fmoc-Ala-OH, Fmoc-Ile-OH, Fmoc-Phe- OH, Fmoc-Glu(OtBu)-OH, Fmoc-Lys(Dde)-OH, Fmoc-Ala-OH, Fmoc-Ala-OH, Fmoc-Gln(Trt)-OH, Fmoc-Glu(OtBu)-OH, Fmoc-Gly-OH, Fmoc-Leu-OH, F sequential coupling of Fmoc-Tyr(tBu)-OH, Fmoc-Ser(tBu)-OH, Fmoc-Ser(tBu)-OH, Fmoc-Val-OH, Fmoc-Asp(OtBu)-OH, Fmoc-Ser(tBu)-OH, Fmoc-Thr(tBu)-OH, Fmoc-Phe-OH, Fmoc-Thr(tBu)-OH, Fmoc-Phe-OH, Fmoc-Thr(tBu)-OH, Glu(OtBu)-OH, Fmoc-Gly-OH, Fmoc-D-Ala-OH and Boc-His(Trt)-OH; d) removal of the lysine side chain protecting group Dde followed by coupling with Fmoc-Glu-OtBu, followed by Fmoc deprotection and coupling with palmitic acid; and e) Cleavage of the peptide from the resin to obtain linear D-liraglutide The present invention relates to a method, comprising: In one embodiment, the process may include purifying D-liraglutide to obtain purified D-liraglutide.

[0013] In one aspect, the disclosure provides a process for preparing a D-semaglutide analogue in which the amino acid at position 2 of native semaglutide is replaced with D-alanine, comprising: a) anchoring Fmoc-Gly-OH to a resin and capping it; b) selectively deprotecting the amino groups; c) Fragments Fmoc-Arg(Pbf)OH, Fmoc-Gly-OH, Fmoc-Arg(Pbf)OH, Fmoc-Val-OH, Fmoc-Leu-OH, Fmoc-Trp(Boc)-OH, Fmoc-Ala-OH, Fmoc-Ile-OH, Fmoc-Phe- OH, Fmoc-Glu(OtBu)-OH, Fmoc-Lys(Dde)-OH, Fmoc-Ala-OH, Fmoc-Ala-OH, Fmoc-Gln(Trt)-OH, Fmoc-Glu(OtBu)-OH, Fmoc-Gly-OH, Fmoc-Leu-OH, F sequential coupling of Fmoc-Tyr(tBu)-OH, Fmoc-Ser(tBu)-OH, Fmoc-Ser(tBu)-OH, Fmoc-Val-OH, Fmoc-Asp(OtBu)-OH, Fmoc-Ser(tBu)-OH, Fmoc-Thr(tBu)-OH, Fmoc-Phe-OH, Fmoc-Thr(tBu)-OH, Fmoc-Phe-OH, Fmoc-Thr(tBu)-OH, Glu(OtBu)-OH, Fmoc-Gly-OH, Fmoc-D-Ala-OH and Boc-His(Trt)-OH; d) removal of the lysine side chain protecting group Dde, followed by coupling with the sequence Fmoc-PEG2-CH2-COOH, Fmoc-Glu-OtBu, followed by Fmoc deprotection and coupling with oxaoctadecanoic acid; and e) Cleavage of the peptide from the resin to obtain linear D-semaglutide The present invention provides a process including:

[0014] In one aspect, the process may include purifying D-semaglutide to obtain purified D-semaglutide. In another aspect, the present disclosure provides suitable dosage forms comprising the GLP-1 analogs of the present disclosure, which may be suitable for administration by oral or parenteral routes. In another aspect, the present disclosure provides suitable dosage forms comprising the liraglutide or semaglutide analogues provided by the present disclosure, which may be suitable for administration by oral or parenteral routes.

[0015] In one aspect, the present disclosure provides a method of lowering glucose levels in a patient in need thereof, comprising administering a therapeutically effective amount of a GLP-1 analog of the present disclosure. In one aspect, the present disclosure provides a method of lowering glucose levels in a patient in need thereof, comprising administering a therapeutically effective amount of a liraglutide or semaglutide analogue of the present disclosure.

[0016] Various objects, features, aspects and advantages of the present subject matter will become more apparent from the following detailed description of preferred embodiments. The following drawings form part of the present specification and are included to further illustrate aspects of the present disclosure, which may be better understood by reference to these drawings in combination with the detailed description of specific embodiments presented herein. [Brief explanation of the drawings]

[0017] [Figure 1] FIG. 1 is a flow chart illustrating a protocol for the preparation of D-liraglutide, including the steps shown in Scheme 1, according to one exemplary embodiment of the present disclosure. [Figure 2]FIG. 2 is a flow chart illustrating a protocol for the preparation of D-semaglutide, including the steps shown in Scheme 2, according to one exemplary embodiment of the present disclosure. [Figure 3] FIG. 3 is the RP-HPLC profile of liraglutide. [Figure 4] FIG. 4 is an RP-HPLC profile of D-liraglutide according to one exemplary embodiment of the present disclosure. [Figure 5] FIG. 5 shows a chromatogram profile during the purification of liraglutide. [Figure 6] FIG. 6 is a chromatogram profile during the purification of D-liraglutide according to one exemplary embodiment of the present disclosure. [Figure 7] FIG. 7 is the RP-HPLC profile of purified liraglutide. [Figure 8] FIG. 8 is an RP-HPLC profile of purified D-liraglutide according to one of the exemplary embodiments of the present disclosure. [Figure 9] 9 is a graph showing a comparison of EC50 values ​​of the reference product Victoza, liraglutide, and D-liraglutide, where SPL1 represents liraglutide and SPL2 represents D-liraglutide according to one of the exemplary embodiments of the present disclosure. [Figure 10] 10 is a graph showing a comparison of the PK profiles of liraglutide and D-liraglutide, where CL represents liraglutide and TL represents D-liraglutide according to one of the exemplary embodiments of the present disclosure. [Figure 11] FIG. 11(a) is a graph showing the PK profile of orally administered D-liraglutide according to one exemplary embodiment of the present disclosure, and FIG. 11(b) is a graph showing the PK profile of the reference product Victoza and D-liraglutide according to one exemplary embodiment of the present disclosure administered subcutaneously. DETAILED DESCRIPTION OF THE INVENTION

[0018] The following is a detailed description of embodiments of the present disclosure. The embodiments have been described in sufficient detail to clearly communicate the present disclosure. However, the many details provided are not intended to limit the anticipated variations of the embodiments. On the contrary, the intention is to cover all modifications, equivalents, and alternatives falling within the spirit and scope of the present disclosure as defined by the appended claims.

[0019] All publications herein are incorporated by reference to the same extent as if each individual publication or patent application was specifically and individually indicated to be incorporated by reference. In the event that a definition or usage of a term in an incorporated reference contradicts or is inconsistent with a definition of that term made herein, the definition of that term made herein shall apply and the definition of that term in the reference shall not apply. Throughout this specification, references to "one embodiment" or "an embodiment" mean that a particular feature, structure, or characteristic described in connection with that embodiment is included in at least one embodiment. Thus, the appearances of the phrases "in one embodiment" or "in an embodiment" in various places throughout this specification are not necessarily all referring to the same embodiment. Furthermore, the particular features, structures, or characteristics may be combined in any suitable manner in one or more embodiments.

[0020] In some embodiments, numbers expressing properties such as amounts of ingredients, concentrations, reaction conditions, and the like, used in describing and claiming particular embodiments of the invention are understood to be optionally modified by the term "about." Accordingly, in some embodiments, the numerical parameters set forth in the specification and attached claims are approximations that may vary depending upon the desired properties sought to be obtained by a particular embodiment. In some embodiments, the numerical parameters should be construed in light of the number of reported significant digits and by applying ordinary rounding techniques. Notwithstanding that the numerical ranges and parameters setting forth the broad scope of some embodiments of the invention are approximations, the numerical values ​​set forth in the specific examples are reported as precisely as possible. The numerical values ​​presented in some embodiments of the invention may contain certain errors necessarily resulting from the standard deviations associated with their respective testing measurements.

[0021] As used herein and throughout the appended claims, the meanings of "a," "an," and "the" include plural references unless the context clearly indicates otherwise. Also, as used herein, the meaning of "in" includes "in" and "on" unless the context clearly indicates otherwise. Unless the context requires otherwise, throughout the remainder of this specification, the word "comprise" and its variations, such as "comprises" and "comprising," should be interpreted in the same open and inclusive sense as "including but not limited to."

[0022] The recitation of ranges of values ​​herein is merely intended to serve as a shorthand method of referring individually to each separate value falling within the range. Unless otherwise indicated herein, each separate value is incorporated herein as if it were individually set forth herein. All methods described herein can be performed in any suitable order unless otherwise indicated herein or a different order is clearly contrary to context. The use of any examples or exemplary language (e.g., "for example," "etc.") in connection with specific embodiments herein is intended to more clearly illustrate the invention and does not limit the scope of the invention as claimed herein. No language in the specification should be construed as indicating any non-claimed element essential to the practice of the invention. Groupings of alternative elements or embodiments of the invention disclosed herein are not to be construed as limiting. Each group member may be referred to and claimed individually or in any combination with other members in the group or other elements described herein. One or more members of a group may be included in or deleted from a group for reasons of convenience and / or patentability. When such inclusion or deletion occurs, the specification shall be deemed to include the modified group as such, and thus satisfy the recitation of all Markush groups used in the appended claims.

[0023] The following description and the embodiments described therein are provided for the purpose of illustrating one or more examples of particular embodiments of the principles and aspects of the present disclosure. These examples are provided for the purpose of illustration and not for the purpose of limitation of the principles and the present disclosure. It should also be understood that the present disclosure can be embodied in various ways, for example, as a system, a method, or a device. These implementations or any other form the present disclosure can take may be referred to herein as a "process." In general, the order of steps in the disclosed processes may be varied within the scope of the present invention. The titles and summaries of the invention provided herein are for convenience only and do not interpret the scope or meaning of the embodiments.

[0024] The following discussion describes many example embodiments of the inventive subject matter. Although each embodiment describes a single combination of inventive elements, it is intended that the inventive subject matter encompass all possible combinations of the disclosed elements. Thus, if one embodiment includes elements A, B, and C and a second embodiment includes elements B and D, it is intended that the inventive subject matter encompass any remaining combination of A, B, C, or D, even if not explicitly disclosed.

[0025] Various terms used in this specification are set forth below. Unless a term used in the claims is defined below, that term should be given the broadest definition that one of ordinary skill in the art would give to that term as it appears in printed publications and issued patents at the time of filing.

[0026] The term "analog," as used herein, refers to a compound that is structurally similar to another compound but differs from that compound with respect to certain components. Such analogs may have significantly different physical, chemical, biochemical, or pharmacological properties.

[0027] The abbreviations used herein refer to the following full forms: Boc: t-butyloxycarbonyl DCM: dichloromethane Dde: 1-(4,4-dimethyl-2,6-dioxocyclohex-1-ylidene)ethyl DIC: N,N'-diisopropylcarbodiimide DIPEA: Diisopropylethylamine DMF: dimethylformamide DODT: 2,2'-(ethylenedioxy)diethanethiol Fmoc: 9-fluorenylmethoxycarbonyl HBTU: Hexafluorophosphate benzotriazole tetramethyluronium HOBt: N-hydroxybenzotriazole HPLC: High-performance liquid chromatography MTBE: Methyl t-butyl ether OtBu: tert-butyl ester tBu: tert-butyl TFA: Trifluoroacetic acid Trt: Trityl 2-CTC: 2-chlorotrityl chloride HCl: Hydrochloric acid mL: milliliter g: grams ℃: Celsius h:hour min:minutes IPA: Isopropanol vol: volume RT: room temperature Mmol: millimolar TIPS: Triisopropylsilane A o :Angstroms HPLC: High-performance liquid chromatography

[0028] The present disclosure relates to synthetic analogs of (glp-1) receptor agonists. In a general embodiment, the disclosure provides analogs of glucagon-like peptide-1 (glp-1) receptor agonists. In certain embodiments, the present disclosure provides analogs of glucagon-like peptide-1 (glp-1) receptor agonists, in which the amino acid at position 2 of the native glucagon-like peptide-1 (glp-1) receptor agonist is replaced with D-alanine. Analogs of glucagon-like peptide-1 (glp-1) receptor agonists, in which the amino acid at position 2 of a natural glucagon-like peptide-1 (glp-1) receptor agonist is replaced with D-alanine, are advantageous over natural glucagon-like peptide-1 (glp-1) receptor agonists, for example, they may have better bioavailability and enhanced efficacy than the respective glucagon-like peptide-1 (glp-1) receptor agonists. In one embodiment, the present disclosure provides an analog of a glucagon-like peptide-1 (glp-1) receptor agonist, wherein the glucagon-like peptide-1 (glp-1) receptor agonist is liraglutide or semaglutide.

[0029] In one embodiment, the present disclosure discloses synthetic analogs of liraglutide that can retain the biological activity of liraglutide. In one embodiment, the present disclosure discloses synthetic analogues of semaglutide that can retain the biological activity of semaglutide. Synthetic analogs of liraglutide are also referred to herein as GLP-1 analogs, GLP-A analogs, liraglutide analogs, liraglutide analogs, or D-liraglutide, analogs of a glucagon-like peptide-1 (glp-1) receptor agonist, and such terms are used interchangeably throughout. Synthetic analogues of semaglutide are also referred to herein as GLP-1 analogues, GLP-A analogues, semaglutide analogues, semaglutide analogues, or D-semaglutide, an analogue of a glucagon-like peptide-1 (glp-1) receptor agonist, and such terms are used interchangeably throughout. In another embodiment, the present disclosure discloses synthetic analogs of liraglutide that can be easily synthesized by solid phase peptide synthesis. In another embodiment, the present disclosure discloses synthetic analogues of semaglutide that can be easily synthesized by solid phase peptide synthesis.

[0030] In one embodiment, the present disclosure provides a process for preparing a synthetic analog of a glucagon-like peptide-1 (glp-1) receptor agonist in which the amino acid at position 2 of the naturally occurring glucagon-like peptide-1 (glp-1) receptor agonist is replaced with D-alanine. In one embodiment, the present disclosure provides a process for preparing a synthetic analog of liraglutide in which the amino acid at position 2 of the natural glucagon-like peptide-1 (glp-1) receptor agonist is replaced with D-alanine. In one embodiment, the present disclosure provides a process for preparing D-liraglutide, in which the amino acid at position 2 of native liraglutide is replaced with D-alanine, comprising: a) anchoring Fmoc-Gly-OH to a resin and capping it; b) selectively deprotecting the amino groups; c) Fragments Fmoc-Arg(Pbf)OH, Fmoc-Gly-OH, Fmoc-Arg(Pbf)OH, Fmoc-Val-OH, Fmoc-Leu-OH, Fmoc-Trp(Boc)-OH, Fmoc-Ala-OH, Fmoc-Ile-OH, Fmoc-Phe- OH, Fmoc-Glu(OtBu)-OH, Fmoc-Lys(Dde)-OH, Fmoc-Ala-OH, Fmoc-Ala-OH, Fmoc-Gln(Trt)-OH, Fmoc-Glu(OtBu)-OH, Fmoc-Gly-OH, Fmoc-Leu-OH, F sequential coupling of Fmoc-Tyr(tBu)-OH, Fmoc-Ser(tBu)-OH, Fmoc-Ser(tBu)-OH, Fmoc-Val-OH, Fmoc-Asp(OtBu)-OH, Fmoc-Ser(tBu)-OH, Fmoc-Thr(tBu)-OH, Fmoc-Phe-OH, Fmoc-Thr(tBu)-OH, Fmoc-Phe-OH, Fmoc-Thr(tBu)-OH, Glu(OtBu)-OH, Fmoc-Gly-OH, Fmoc-D-Ala-OH and Boc-His(Trt)-OH; d) removal of the lysine side chain protecting group Dde followed by coupling with Fmoc-Glu-OtBu, followed by Fmoc deprotection and coupling with palmitic acid; and e) Cleavage of the peptide from the resin to obtain linear D-liraglutide The present invention provides a process including:

[0031] In one embodiment, the method may include purifying D-liraglutide to obtain purified D-liraglutide. In another embodiment, the present disclosure provides a process for preparing D-liraglutide, comprising the steps shown in Scheme 1 (FIG. 1).

[0032] In one embodiment, the disclosure provides a process for preparing a D-semaglutide analogue in which the amino acid at position 2 of native semaglutide is replaced with D-alanine, comprising: a) anchoring Fmoc-Gly-OH to a resin and capping it; b) selectively deprotecting the amino groups; c) Fragments Fmoc-Arg(Pbf)OH, Fmoc-Gly-OH, Fmoc-Arg(Pbf)OH, Fmoc-Val-OH, Fmoc-Leu-OH, Fmoc-Trp(Boc)-OH, Fmoc-Ala-OH, Fmoc-Ile-OH, Fmoc-Phe- OH, Fmoc-Glu(OtBu)-OH, Fmoc-Lys(Dde)-OH, Fmoc-Ala-OH, Fmoc-Ala-OH, Fmoc-Gln(Trt)-OH, Fmoc-Glu(OtBu)-OH, Fmoc-Gly-OH, Fmoc-Leu-OH, F sequential coupling of Fmoc-Tyr(tBu)-OH, Fmoc-Ser(tBu)-OH, Fmoc-Ser(tBu)-OH, Fmoc-Val-OH, Fmoc-Asp(OtBu)-OH, Fmoc-Ser(tBu)-OH, Fmoc-Thr(tBu)-OH, Fmoc-Phe-OH, Fmoc-Thr(tBu)-OH, Fmoc-Phe-OH, Fmoc-Thr(tBu)-OH, Glu(OtBu)-OH, Fmoc-Gly-OH, Fmoc-D-Ala-OH and Boc-His(Trt)-OH; d) removal of the lysine side chain protecting group Dde, followed by coupling with the sequence Fmoc-PEG2-CH2-COOH, Fmoc-Glu-OtBu, followed by Fmoc deprotection and coupling with oxaoctadecanoic acid; and e) Cleavage of the peptide from the resin to obtain linear D-semaglutide The present invention provides a process including:

[0033] In one embodiment, the process may include a step of purifying D-semaglutide to obtain purified D-semaglutide. In one embodiment, the present disclosure provides a process for preparing D-semaglutide, comprising the steps shown in Scheme 2 (Figure 2).

[0034] In one embodiment, the solid phase is a resin. In one embodiment, the resin is selected from, but not limited to, 2-chlorotrityl chloride (2-CTC), Sasrin, TentaGel S, TentaGel TGA, Rink, Wang, AmphiSpheres, and other suitable resins. In one embodiment, the coupling agent is selected from, but not limited to, 1-hydroxybenzotriazole (HOBt), N,N-diisopropylcarbodiimide (DIC), hexafluorophosphate benzotriazole tetramethyluronium (HBTU), N,N-diisopropylethylamine (DIPEA), benzotriazol-1-yl-oxy-tris(dimethyl-amino)-phosphonium hexafluorophosphate (BOP), O-(7-azabenzotriazol-1-yl)-1,1,3,3-tetramethyluronium hexafluorophosphate (HATU), and combinations thereof. In one embodiment, the solvent for the coupling reaction is selected from, but not limited to, DMF, pyridine, acetic anhydride, methanol, ethanol, isopropanol, dichloroethane, 1,4-dioxane, 2-methyltetrahydrofuran, N-methyl-2-pyrrolidinone (NMP), ethyl acetate, acetonitrile, acetone, etc., or combinations thereof.

[0035] In one embodiment, the amino group can be selectively deprotected by methods known in the art, for example, using a mixture of piperidine, DBU and dichloromethane in a suitable solvent such as DMF. In one embodiment, the peptide formed can be cleaved from the resin using chemicals selected from, but not limited to, difluoroacetic acid, trifluoroacetic acid, and the like.

[0036] In one embodiment, the purification process for the GLP-1 analogue selected from D-liraglutide or D-semaglutide can be carried out by processes well known in the art, including but not limited to preparative reverse-phase HPLC, ion exchange chromatography, size exclusion chromatography, affinity chromatography, etc. The synthetic analogues of GLP-1 provided by the present disclosure, namely D-liraglutide and D-semaglutide, may have better pharmacokinetic profiles compared to natural liraglutide and semaglutide, respectively. The GLP-1 analogues provided by the present disclosure, namely D-liraglutide and D-semaglutide, may be advantageous in reducing patient burden, for example by reducing the frequency of administration of dosage forms containing liraglutide or semaglutide analogues. The GLP-1 analogues of the present disclosure, namely D-liraglutide or D-semaglutide, can be used to treat metabolic disorders such as diabetes and obesity. The GLP-1 analogues of the present disclosure, namely D-liraglutide or D-semaglutide, each may offer advantages over conventional GLP-1s in that they can be administered less frequently than conventional GLP-1s, providing convenience to patients and thereby increasing patient compliance, as well as enabling effective glycemic control over a longer period of time.

[0037] The GLP-1 analogues D-liraglutide or D-semaglutide according to the present disclosure may be long-acting analogues suitable for weekly, biweekly or monthly administration.

[0038] The GLP-1 analogues D-liraglutide or D-semaglutide can exist in the form of a base, a salt thereof, or a mixture thereof. Representative examples of salts include salts with suitable inorganic acids such as hydrochloric acid, hydrobromic acid, etc. Representative examples of salts also include salts with organic acids such as formic acid, acetic acid, propionic acid, lactic acid, tartaric acid, ascorbic acid, etc. Representative examples of salts also include salts with bases such as triethanolamine, diethylamine, meglumine, arginine, alanine, leucine, diethylethanolamine, triethylamine, tromethamine, choline, trimethylamine, taurine, benzamine, methylamine, dimethylamine, trimethylamine, methylethanolamine, propylamine, isopropylamine, adenine, guanine, cytosine, thymine, uracil, thymine, xanthine, hypoxanthine, etc. However, one of ordinary skill in the art will understand that any other synthetic moiety that is not degradable by DPP-IV, as known to one of ordinary skill in the art, can be used without departing from the scope and spirit of the present disclosure.

[0039] In another embodiment, the GLP-1 analogues provided by the present disclosure, i.e., D-liraglutide or D-semaglutide, may be provided in the form of a lyophilized mixture comprising D-liraglutide or D-semaglutide together with a parenterally acceptable amine base. This lyophilized mixture may be prepared by mixing the GLP-1 analogues D-liraglutide or D-semaglutide, or a pharmaceutically acceptable salt thereof, with a parenterally acceptable amine base in water for injection to form a solution, and lyophilizing this solution to form a lyophilized mixture. The parenterally acceptable amine base may be selected from triethanolamine, diethylamine, meglumine, ornithine, lysine, arginine, alanine, leucine, diethylethanolamine, olamine, triethylamine, tromethamine, glucosamine, choline, trimethylamine, taurine, benzamine, trimethylammonium hydroxide, epolamine, methylamine, dimethylamine, trimethylamine, methylethanolamine, propylamine, isopropylamine, etc.

[0040] The present disclosure also provides the use of the GLP-1 analogs provided by the present disclosure, i.e., the D-liraglutide and D-semaglutide of the present disclosure, in the treatment of metabolic diseases. In a preferred embodiment, the semaglutide analogs of the present disclosure may be suitable for use in the treatment of diabetes. In another embodiment, the semaglutide analogs of the present disclosure may be suitable for use in the treatment of obesity. In another embodiment, the GLP-1 analogues of the present disclosure, i.e., D-liraglutide or D-semaglutide, may be suitable for use in reducing blood glucose levels over a period of at least one week in a patient in need thereof. In another embodiment, the present disclosure provides dosage forms suitable for administering the GLP-1 analogues of the present disclosure, namely D-liraglutide or D-semaglutide, by oral or parenteral routes.

[0041] The GLP-1 analogues of the present disclosure, i.e., D-liraglutide or D-semaglutide, may be formulated into a suitable parenteral dosage form. The GLP-1 analogues of the present disclosure, D-liraglutide or D-semaglutide, or a composition comprising same, or a dosage form comprising same, may be administered by subcutaneous or intramuscular injection. The GLP-1 analogues D-liraglutide or D-semaglutide of the present disclosure may be formulated into a suitable oral dosage form. The GLP-1 analogues D-liraglutide or D-semaglutide of the present disclosure, or compositions comprising same, or oral dosage forms comprising same, may be orally administered at a frequency according to the needs of the subject in need of GLP-1 administration.

[0042] The GLP-1 analogues D-liraglutide or D-semaglutide of the present disclosure can maintain therapeutic levels for extended periods of time, which may be as long as one week or two weeks or one month, following a single dose. The GLP-1 analogues D-liraglutide or D-semaglutide of the present disclosure can be used to treat diabetes by administering the GLP-1 analogue, or a composition or dosage form comprising same, once weekly, once every two weeks or once monthly. In another embodiment, the present disclosure provides a method of lowering glucose levels in a patient in need thereof, comprising administering a therapeutically effective amount of a GLP-1 analogue of the present disclosure, D-liraglutide or D-semaglutide. According to another embodiment, the present invention provides a pharmaceutical composition comprising as an active ingredient the GLP-1 analogues D-liraglutide or D-semaglutide of the present disclosure, together with one or more pharmaceutically acceptable carriers or excipients.

[0043] According to another embodiment, compositions can be prepared by combining one or more analogs described herein, or pharmaceutically acceptable salts or tautomers thereof, with pharmaceutically acceptable carriers or the like to treat or ameliorate various GLP-1-related conditions. Pharmaceutical compositions of the present disclosure can be prepared by methods well known in the art, such as conventional granulation, mixing, dissolving, encapsulation, lyophilization, emulsification, or wet granulation processes. The compositions may be in the form of, for example, granules, powders, tablets, capsules, syrups, suppositories, injections, emulsions, elixirs, suspensions, or solutions. The compositions can be formulated for various routes of administration, such as oral, transmucosal, rectal, topical, or subcutaneous administration, as well as intrathecal, intravenous, intramuscular, intraperitoneal, intranasal, intraocular, or intracerebroventricular injection. One or more compounds of the present invention can also be administered locally rather than systemically, for example, by injection as a sustained-release formulation.

[0044] According to another embodiment, the GLP-1 analogues D-liraglutide or D-semaglutide of the present disclosure may be used alone or in combination with one or more additional therapeutically active agents. In one embodiment, the present invention provides a method of treating a GLP-1 mediated disease, disorder or syndrome in a subject, comprising administering an effective amount of a GLP-1 analogue of the present disclosure, D-liraglutide or D-semaglutide. In another embodiment, the present invention provides a method for treating a GLP-1 mediated disease, disorder, or syndrome in a subject, comprising the step of administering an effective amount of the GLP-1 analogues D-liraglutide or D-semaglutide, wherein the disease is selected from the group consisting of type 2 diabetes, type 1 diabetes, impaired glucose tolerance, hyperglycemia, metabolic syndrome (syndrome X and / or insulin resistance syndrome), diabetes, metabolic acidosis, arthritis, cataracts, diabetic neuropathy, diabetic nephropathy, The method is provided for treating diabetic retinopathy, diabetic cardiomyopathy, obesity, conditions aggravated by obesity, hypertension, hyperlipidemia, atherosclerosis, osteoporosis, osteopenia, frailty, bone loss, fractures, acute coronary syndrome, short stature due to growth hormone deficiency, infertility due to polycystic ovary syndrome, anxiety, depression, insomnia, chronic fatigue, epilepsy, eating disorders, chronic pain, alcoholism, diseases related to intestinal motility, ulcers, irritable bowel syndrome, inflammatory bowel syndrome, or short bowel syndrome.

[0045] In another embodiment, the invention provides the use of the GLP-1 analogues D-liraglutide or D-semaglutide for the treatment of a disease selected from type 2 diabetes, type 1 diabetes, impaired glucose tolerance, hyperglycemia, metabolic syndrome (syndrome X and / or insulin resistance syndrome), diabetes, metabolic acidosis, arthritis, cataracts, diabetic neuropathy, diabetic nephropathy, diabetic retinopathy, diabetic cardiomyopathy, obesity, conditions exacerbated by obesity, hypertension, hyperlipidemia, atherosclerosis, osteoporosis, osteopenia, frailty, bone loss, fractures, acute coronary syndrome, short stature due to growth hormone deficiency, infertility due to polycystic ovary syndrome, anxiety, depression, insomnia, chronic fatigue, epilepsy, eating disorders, chronic pain, alcoholism, diseases related to intestinal motility, ulcers, irritable bowel syndrome, inflammatory bowel syndrome or short bowel syndrome.

[0046] While the foregoing is a description of various embodiments of the present disclosure, other and further embodiments of the present disclosure may be devised without departing from the basic scope thereof. The scope of the present disclosure is determined by the appended claims. The present disclosure is not limited to the described embodiments, variations, or examples, but includes those which, when combined with information and knowledge available to those skilled in the art, enable one to make and use the invention. [Example]

[0047] The present invention will be further described in the form of the following examples, however, it should be understood that the following examples are illustrative only and should not be construed as imposing limitations on the scope of the invention. Example 1 Synthesis of D-liraglutide Step 1: Anchoring Fmoc-Gly-CTC to the resin Fmoc-Gly-CTC resin with a substitution degree of 0.35 mmol / g was weighed and loaded onto a solid-phase reaction column, followed by washing twice with DMF and allowing it to swell in DMF for 30 minutes. Step 2: Deprotect the amino acid After removing the Fmoc protection with 20% piperidine, the resin was washed four times with DMF and twice with DCM. The resin was tested by the ninhydrin test, and the removal of Fmoc was indicated by the appearance of the color of the resin. Step 3: Successive coupling of other Fmoc-protected amino acids Fmoc-Arg(Pbf)-OH (6.0 mmol), HOBt (7.2 mmol), and DIC (7.2 mmol) were dissolved in a 1:1 volumetric mixture of DCM and DMF and loaded onto a solid-phase reaction column. The reaction was allowed to proceed at room temperature for 2 hours. The end point of the reaction was determined by the ninhydrin test. A colorless, transparent resin indicated complete reaction, whereas a colored resin indicated incomplete reaction, requiring an additional hour of reaction. These criteria were applied to determine the end point using the ninhydrin test. The above step 2 and the corresponding amino acid coupling steps were repeated, and based on the sequence of the peptide backbone of D-liraglutide, Fmoc-Arg(Pbf)OH, Fmoc-Gly-OH, Fmoc-Arg(Pbf)OH, Fmoc-Val-OH, Fmoc-Leu-OH, Fmoc-Trp(Boc)-OH, Fmoc-Ala-OH, Fmoc-Ile-OH, Fmoc-Phe-OH, Fmoc-Glu(OtBu)-OH, Fmoc-Lys(Dde)-OH, Fmoc-Ala-OH, Fmoc-Ala-OH, Fmoc-Gln(Trt)-OH, Fmoc-G lu(OtBu)-OH, Fmoc-Gly-OH, Fmoc-Leu-OH, Fmoc-Tyr(tBu)-OH, Fmoc-Ser(tBu)-OH, Fmoc-Ser(tBu)-OH, Fmoc-Val-OH, Fmoc-Asp(OtBu)-OH, Fmoc-Ser(tBu)-O H, Fmoc-Thr(tBu)-OH, Fmoc-Phe-OH, Fmoc-Thr(tBu)-OH, Fmoc-Phe-OH, Fmoc-Thr(tBu)-OH, Glu(OtBu)-OH, Fmoc-Gly-OH, Fmoc-D-Ala-OH and Boc-His(Trt)-OH were coupled successively.

[0048] Step 4: Preparation of the Dde-deprotected resin fragment The resin fragment obtained after successive couplings in step 3 above was added to a clear mixture of 3% hydrazine hydrate in DMF lot-1 (10 vol). The suspension was gently agitated for 10 min at 25-30°C under nitrogen bubbling and gentle stirring. The solvent was drained, and the resin was added to a clear mixture of 3% hydrazine hydrate in DMF lot-2 (10 vol). The suspension was agitated for 10 min at 25-30°C. The solvent was drained, and the resin was washed with DMF (2 × 10 vol), IPA (1 × 10 vol), and DMF (2 × 10 vol). Completion of the Dde deprotection was confirmed by the Kaiser color test.

[0049] Step 5: Coupling of Fmoc-Glu-OtBu with palmitic acid Step (5a): Coupling of Fmoc-Glu-OtBu A clear mixture of Fmoc-Glu-OtBu (2.0 equiv.), N,N-diisopropylcarbodiimide (DIC) (2.0 equiv.), and 1-hydroxybenzotriazole (HOBt) (2.0 equiv.) in DMF (10 vol.) was added to the resin. The suspension was gently agitated under nitrogen bubbling and gentle stirring at 45–55°C for 30 min. The reaction progress was monitored by the Kaiser color test. After completion of the reaction, the reaction solvent was drained and the resin was washed with DMF (4 × 10 vol.). Step (5b): Fmoc deprotection The resin was added to a clear mixture of 20% piperidine in DMF lot-1 (10 vol). The suspension was gently agitated for 10 minutes at 25-30°C under nitrogen bubbling and gentle stirring. The solvent was drained, and the resin was added to a clear mixture of 20% piperidine in DMF lot-2 (10 vol). The suspension was agitated for 10 minutes at 25-30°C. The solvent was drained, and the resin was washed with DMF (2 × 10 vol), IPA (1 × 10 vol), and DMF (2 × 10 vol). Completion of the Fmoc deprotection was confirmed by a Kaiser color test. Step (5c): Coupling of palmitic acid A clear mixture of palmitic acid (2.0 equiv.), N,N-diisopropylcarbodiimide (DIC) (2.0 equiv.), and 1-hydroxybenzotriazole (HOBt) (2.0 equiv.) in DMF (10 vol.) was added to the resin. The suspension was gently agitated under nitrogen bubbling and gentle stirring at 45–55°C for 30 min. The reaction progress was monitored by the Kaiser color test. After completion of the reaction, the reaction solvent was drained and the resin was washed with DMF (4 × 10 vol.).

[0050] Step 6: Preparation of D-liraglutide The 2-CTC resin-bound protected fragment obtained in Step 5 was placed in a peptide synthesis flask. The resin was suspended in dichloromethane (DCM) (10 vol) for 10 minutes without stirring. The resin was added to a mixture of TFA:TIPS:DODT:water (8.5:0.5:0.5:0.5 vol). The suspension was gently agitated at 25-30°C for 3.0 hours under nitrogen bubbling and gentle stirring. The resin was filtered through a sintered funnel. The filtrate was added to a pre-cooled MTBE mixture at 0-10°C. After the addition was complete, the reaction mixture was stirred at 0-35°C for 1.0 hour, at which time an off-white solid precipitated. The precipitated solid was then filtered through a Buchner funnel and washed with MTBE. The suction-dried solid was then dried to constant weight in a vacuum oven at 35-40°C to obtain D-liraglutide.

[0051] Step 7: Purification of D-liraglutide Step (7a): Purification-1 3.6 g of D-liraglutide obtained after global deprotection was dissolved in 300 mL of buffer A, and the pH was adjusted to 8.5-9.5 with approximately 0.5 mL of ammonium hydroxide solution. The following parameters were followed during purification: Column specifications: 250 x 50 mm SS Packing material (specifications: C-18 (3rd generation), 10μ, 100Å Mobile phase A: 0.01M ammonium bicarbonate, Mobile phase B: acetonitrile, Pooling criteria: fractions with HPLC purity ≥ 85% and a maximum concentration of a single impurity ≤ 3% were pooled for purification 2. Fractions with HPLC purities ≦85% and ≧60% were pooled for repurification.

[0052] Step (7b): Purification-2 The pooled fractions containing 900 mg of peptide obtained in purification-1 were further diluted with an equal volume of purified water and purified according to the following parameters: Column specifications: 250 x 50 mm SS Packing material specifications: C-18 (3rd generation), 10μ, 100Å Mobile phase A: 0.1% TFA in water, Mobile phase B: acetonitrile, Pooling criteria: fractions with HPLC purity ≥ 96% and a maximum concentration of a single impurity ≤ 0.5% were pooled for purification 3. Fractions with HPLC purities ≦96% and ≧85% were pooled for repurification.

[0053] Step (7c): Purification-3 The pooled fractions containing 1200 mg of peptide obtained in purification-2 were further diluted with an equal volume of purified water and purified as follows: Column specifications: 250 x 50 mm SS Packing material specifications: C-18 (3rd generation), 10μ, 100Å Mobile phase A: 0.05% ammonium hydroxide in water, Mobile phase B: acetonitrile, Mobile phase C: 3% ammonium acetate in water, Mobile phase D: purified water Pooling criteria: fractions with HPLC purity ≥ 98% and a maximum concentration of a single impurity ≤ 0.3% were pooled for concentration. Fractions with HPLC purities ≦98% and ≧96% were pooled for repurification. The pooled fractions obtained in purification step 3 were concentrated and subjected to lyophilization to obtain pure D-liraglutide (I) as an off-white to white powder. The HPLC purity of the obtained D-liraglutide was 99.0% or more, and the isolated yield was in the range of 9-12%.

[0054] Example 2 Synthesis of D-semaglutide D-semaglutide was synthesized according to the following process. Step 1 to Step 4: The process described in Example 1 was followed for the synthesis of D-liraglutide preparation according to Steps 1 to 4.

[0055] Step 5: Coupling of Fmoc-PEG2-CH2-COOH, Fmoc-PEG2-CH2-COOH, Fmoc-Glu-OtBu, and 18-tBu-18-oxaoctadecanoic acid The coupling was carried out stepwise according to the stepwise scheme shown below: Step (5a): Coupling of Fmoc-PEG-CH-COOH A clear mixture of Fmoc-PEG2-CH2-COOH (2.0 equiv.), N,N-diisopropylcarbodiimide (DIC) (2.0 equiv.), and 1-hydroxybenzotriazole (HOBt) (2.0 equiv.) in DMF (10 vol.) was added to the resin. The suspension was gently agitated under nitrogen bubbling and gentle stirring at 45–55°C for 30 min. The reaction progress was monitored by the Kaiser color test. After completion of the reaction, the reaction solvent was drained and the resin was washed with DMF (4 × 10 vol.). Step (5b): Fmoc deprotection The resin was added to a clear mixture of 20% piperidine in DMF lot-1 (10 vol). The suspension was gently agitated for 10 minutes at 25-30°C under nitrogen bubbling and gentle stirring. The solvent was drained, and the resin was added to a clear mixture of 20% piperidine in DMF lot-2 (10 vol). The suspension was agitated for 10 minutes at 25-30°C. The solvent was drained, and the resin was washed with DMF (2 × 10 vol), IPA (1 × 10 vol), and DMF (2 × 10 vol). Completion of the Fmoc deprotection was confirmed by a Kaiser color test.

[0056] Step (5c): Coupling of Fmoc-PEG2-CH2-COOH A clear mixture of Fmoc-PEG2-CH2-COOH (2.0 equiv.), N,N-diisopropylcarbodiimide (DIC) (2.0 equiv.), and 1-hydroxybenzotriazole (HOBt) (2.0 equiv.) in DMF (10 vol.) was added to the resin. The suspension was gently agitated under nitrogen bubbling and gentle stirring at 45–55°C for 30 min. The reaction progress was monitored by the Kaiser color test. After completion of the reaction, the reaction solvent was drained and the resin was washed with DMF (4 × 10 vol.). Step (5d): Fmoc deprotection The resin was added to a clear mixture of 20% piperidine in DMF lot-1 (10 vol). The suspension was gently agitated for 10 minutes at 25-30°C under nitrogen bubbling and gentle stirring. The solvent was drained, and the resin was added to a clear mixture of 20% piperidine in DMF lot-2 (10 vol). The suspension was agitated for 10 minutes at 25-30°C. The solvent was drained, and the resin was washed with DMF (2 × 10 vol), IPA (1 × 10 vol), and DMF (2 × 10 vol). Completion of the Fmoc deprotection was confirmed by a Kaiser color test.

[0057] Step (5e): Coupling of Fmoc-Glu-OtBu A clear mixture of Fmoc-Glu-OtBu (2.0 equiv.), N,N-diisopropylcarbodiimide (DIC) (2.0 equiv.), and 1-hydroxybenzotriazole (HOBt) (2.0 equiv.) in DMF (10 vol.) was added to the resin. The suspension was gently agitated under nitrogen bubbling and gentle stirring at 45–55°C for 30 min. The reaction progress was monitored by the Kaiser color test. After completion of the reaction, the reaction solvent was drained and the resin was washed with DMF (4 × 10 vol.). Step (5f): Fmoc deprotection The resin was added to a clear mixture of 20% piperidine in DMF lot-1 (10 vol). The suspension was gently agitated for 10 minutes at 25-30°C under nitrogen bubbling and gentle stirring. The solvent was drained, and the resin was added to a clear mixture of 20% piperidine in DMF lot-2 (10 vol). The suspension was agitated for 10 minutes at 25-30°C. The solvent was drained, and the resin was washed with DMF (2 × 10 vol), IPA (1 × 10 vol), and DMF (2 × 10 vol). Completion of the Fmoc deprotection was confirmed by a Kaiser color test.

[0058] Step (5g): Coupling of 18-tBu-18-oxaoctadecanoic acid A clear mixture of 18-tBu-18-oxaoctadecanoic acid (2.0 equiv.), N,N-diisopropylcarbodiimide (DIC) (2.0 equiv.), and 1-hydroxybenzotriazole (HOBt) (2.0 equiv.) in DMF (10 vol.) was added to the resin. The suspension was gently agitated under nitrogen bubbling and gentle stirring at 45–55°C for 30 min. The reaction progress was monitored by the Kaiser color test. After completion of the reaction, the reaction solvent was drained and the resin was washed with DMF (4 × 10 vol.).

[0059] Step 6: Preparation of D-semaglutide The 2-CTC resin-bound protected fragment obtained in Step 5 was placed in a peptide synthesis flask. The resin was suspended in dichloromethane (DCM) (10 vol) and left without stirring for 10 minutes. The resin was added to a mixture of TFA:TIPS:DODT:water (8.5:0.5:0.5:0.5 vol). The suspension was gently agitated at 25-30°C for 3.0 hours under nitrogen bubbling and gentle stirring. The resin was filtered through a sintered funnel. The filtrate was added to a pre-cooled MTBE mixture at 0-10°C. After the addition was complete, the reaction mixture was stirred at 0-35°C for 1.0 hour, at which time an off-white solid precipitated. The precipitated solid was then filtered through a Buchner funnel and washed with MTBE. The suction-dried solid was then dried to constant weight in a vacuum oven at 35-40°C to obtain D-semaglutide.

[0060] Example 3 Purification of (L-Ala)-liraglutide and (D-Ala)-liraglutide The method for purifying both crude (L-Ala)-liraglutide (natural product) and crude (D-Ala)-liraglutide obtained by solid phase synthesis is characterized by comprising the following steps: Step 1: 100 mg of crude liraglutide obtained by solid phase synthesis was dissolved in 0.01 M ammonium bicarbonate in 25% ammonia solution to obtain a solution of crude liraglutide, which was then filtered through a 0.2 μm filter. Step 2: The solution of both crude liraglutide, (L-Ala)-liraglutide and (D-Ala)-liraglutide, is subjected to a first HPLC purification using a 10*250mm Phenominex C18 (3rd generation) 100A, 10μm column and 0.01M ammonium bicarbonate as mobile phase A and acetonitrile as mobile phase B eluting with a gradient as described in Table 1, and the target peak is collected and analyzed by RP-HPLC for purity and content.

[0061] [Table 1] The RP-HPLC profiles for crude liraglutide and D-liraglutide with purities of 50.4% and 15.1%, respectively, are shown in Figures 3 and 4.

[0062] Chromatogram profiles showing the peaks of interest for both liraglutide and D-liraglutide are shown in Figures 5 and 6. Pooled fractions for liraglutide and D-liraglutide were purified by lyophilization, showing RP-HPLC purities of 93.1% and 90.0%, respectively (Figures 7 and 8, respectively). Details are shown in Table 2 below.

[0063] [Table 2] Example 4 Biological characterization of liraglutide and D-liraglutide The in-vitro efficacy of our products is determined based on the stimulation of adenylate cyclase activity in the rat thyroid c-cell line 6-23 (Clone 6) (ATCC® CRL-1607™). Activation of the GLP-1 receptor initiates a cascade of events that leads to a rapid increase in intracellular cAMP levels. cAMP levels were determined using a cAMP ELISA kit and compared with RMP (Victoza). Statistical analysis was performed using Graph pad Prism software. As shown in Figure 9, the EC50 value observed for the reference (Victoza) was 1.99 ng / mL, and the EC50 values ​​observed for synthetic liraglutide and D-liraglutide were 1.82 ng / ml and 1.43 ng / mL, respectively.

[0064] Example 5 Pharmacokinetic (PK) analysis of liraglutide and D-liraglutide in diabetic (DM-2) Wistar rats Streptozotocin (STZ) is a selective toxicant for pancreatic β cells and is commonly used to model type 2 diabetes mellitus (DM) in multiple species, including Wistar rats. Male Wistar rats aged 12–14 weeks (weight: 300–380 g) were selected for the experiment. Rats were randomly assigned to different groups based on their ad libitum-fed blood glucose and body weight. DM was induced in both control and test groups of Wistar rats by a single intraperitoneal injection of STZ at 60 mg / kg (n=6) over a 2-week period.

[0065] Before STZ injection, basal glucose levels were recorded for all animals. 15 days after STZ treatment, all animals were reassessed for elevated blood levels. After confirming elevated glucose levels, animals were treated with a single dose (5 mg / kg) of liraglutide and D-liraglutide via the subcutaneous route. Blood samples were collected at 0 hours (before dosing), 1, 2, 4, 8, 12, 24, 48, 72, 96, 120, and 144 hours after dosing. At each time point, approximately 0.3 mL of blood was collected from the retroorbital plexus under light isoflurane anesthesia into labeled microfuge tubes. All blood samples were centrifuged at 7000 rpm for 5 minutes at 4°C. After centrifugation, serum was separated and stored at -80°C for further analysis. Quantitative measurement of liraglutide in serum samples was performed using an ELISA kit from Cloud-Clone Corp. (CEV769Ge 96 Tests), which is available for competitive enzyme inhibition assays. All serum samples from both the liraglutide and D-liraglutide groups were diluted 1:100 with sample dilution buffer and tested by ELISA for the presence of liraglutide at various time points using a standard graph.

[0066] As shown in Table 3 and Figure 10, the data obtained after ELISA completion and statistical analysis were analyzed to evaluate the PK parameters (T 1 / 2 , C max , t max , AUC 0-t and MRT) determination values ​​were run using PK solver software using a non-compartmental model.

[0067] [Table 3]

[0068] D-liraglutide was tested to investigate whether slower absorption could extend the dosing interval without affecting expected clinical efficacy, thereby reducing treatment costs, increasing compliance, and benefiting animal welfare. Results show that a significant difference was detected between liraglutide and D-liraglutide (SC half-life (T1 / 2) of 24.77 vs. 54.44 hours, respectively). The AUC value of D-liraglutide was found to be three times higher than that of liraglutide, indicating significantly higher absolute bioavailability for D-liraglutide. The higher Cmax value for D-liraglutide also supports these findings.

[0069] Example 6 Oral Bioavailability of D-Liraglutide Versus Subcutaneous Route A phase 2 pharmacokinetic study was conducted to understand the oral bioavailability of D-liraglutide compared to the subcutaneous route. Proteins and peptides typically exhibit poor oral bioavailability due to extensive enzymatic degradation in the gastrointestinal tract and limited permeation across the gastrointestinal mucosa. The oral bioavailability of proteins and peptides is less than 1%.

[0070] Healthy adult male rats (7-9 weeks old) were randomly assigned to two groups. The control group received 6 mg / kg of Victoza subcutaneously, and the test group received 15 mg / kg of the test molecule, D-liraglutide, orally. Blood samples were collected according to Table 4 to estimate the liraglutide content in the blood for PK comparison.

[0071] [Table 4]

[0072] Estimation of liraglutide in plasma samples: A 50-fold dilution of liraglutide in SD rat plasma was prepared and diluted in assay buffer (HBSS supplemented with IMBX, MgCl2, and Ro). Cells overexpressing GLP-1R [(CHOK1 / GLP1 / Gα15), catalog number M00451, lot number R10081093-12)] were then removed by trypsinization and centrifuged. These cells were then washed with assay buffer. Cells were seeded at 15kJ / well / 15uL, 15μL of double dilution was added, and the plate was incubated at 37°C for 30 minutes. cAMP production was then estimated using a cAMP estimation kit (Promega cAMP-Glo™ Max Assay, catalog number V1682). After 30 minutes of incubation, 20μL of protein kinase A solution was added to the plate and incubated at room temperature for 20 minutes. 50μL of substrate was then added to the plate and incubated at room temperature for 10 minutes. Luminescence was read on a plate reader. Liraglutide content in plasma was back-calculated using the calibration curve generated from each run as shown in Table 5. Gen 5 software was used for back-calculation. Preclinical PK parameters of liraglutide are shown in Table 6.

[0073] [Table 5]

[0074] [Table 6]

[0075] Peptide drugs always pose challenges for oral delivery due to their hydrophobicity and degradation by digestive enzymes. It takes 5–10 minutes for GLP-1 to become physiologically active, and the GLP-1 concentration required for glucose transport is in the single or low double digit pM range. Even after the use of DPP-IV inhibitors, circulating GLP-1 concentrations can reach 50–60 pM, which has been proven to be clinically significant.

[0076] The PK profiles of oral D-liraglutide and subcutaneous Victoza are shown in Figure 11. Oral D-liraglutide demonstrated a cmax of 1.5 ng / mL, corresponding to 400 pM. Given that physiological postprandial activation of GLP-1 R requires 90–150 minutes, and that circulating GLP-1 levels of 60 pM are sufficient to improve glucose tolerance, the 400 pM D-liraglutide levels observed in three animals by the inventors may represent a promising therapeutic option. Although lower potency (2–5-fold) liraglutide was investigated in comparison with D-liraglutide, the circulating concentrations in this study may be sufficient to produce significant improvements in glucose tolerance.

Claims

1. 1. A process for preparing an analogue selected from D-liraglutide and D-semaglutide, comprising: a) anchoring Fmoc-Gly-OH to a resin and capping it; b) selectively deprotecting the amino groups; c) Fragments Fmoc-Arg(Pbf)OH, Fmoc-Gly-OH, Fmoc-Arg(Pbf)OH, Fmoc-Val-OH, Fmoc-Leu-OH, Fmoc-Trp(Boc) -OH, Fmoc-Ala-OH, Fmoc-Ile-OH, Fmoc-Phe-OH, Fmoc-Glu(OtBu)-OH, Fmoc-Lys(Dde)-OH, Fmoc-Ala-OH , Fmoc-Ala-OH, Fmoc-Gln(Trt)-OH, Fmoc-Gly-OH, Fmoc-Glu(OtBu)-OH, Fmoc-Leu-OH, Fmoc-Tyr(tBu) -OH, Fmoc-Ser(tBu)-OH, Fmoc-Ser(tBu)-OH, Fmoc-Val-OH, Fmoc-Asp(OtBu)-OH, Fmoc-Ser(tBu)-OH, F Sequential coupling of Fmoc-Thr(tBu)-OH, Fmoc-Phe-OH, Fmoc-Thr(tBu)-OH, Fmoc-Gly-OH, Glu(OtBu)-OH, Fmoc-D-Ala-OH and Boc-His(Trt)-OH gave Boc-His(Trt)-D-Ala-Glu(OtBu)-Gly-Thr(tBu)-Phe-Thr(tBu)-Se r(tBu)-Asp(OtBu)-Val-Ser(tBu)-Ser(tBu)-Tyr(tBu)-Leu-Glu(OtBu)-Gly-Gln(Trt)-Ala-Ala-Lys Obtaining (Dde)-Glu(OtBu)-Phe-Ile-Ala-Trp(Boc)-Leu-Val-Arg(Pbf)-Gly-Arg(Pbf)-Gly-O-2-CTC, d) for the preparation of D-liraglutide, removal of the lysine side chain protecting group Dde, followed by coupling with Fmoc-Glu-OtBu, followed by Fmoc deprotection and coupling with palmitic acid; or for the preparation of D-semaglutide, removal of the lysine side chain protecting group Dde, followed by Fmoc-PEG2-CH 2 -COOH followed by Fmoc deprotection and coupling with oxaoctadecanoic acid; and e) Cleavage of the peptide from the resin to obtain linear D-liraglutide or D-semaglutide The process includes:

2. 2. The process of claim 1, optionally comprising purifying D-liraglutide or D-semaglutide to obtain purified D-liraglutide or D-semaglutide, respectively.

3. 2. The process of claim 1, wherein the coupling agent is selected from 1-hydroxybenzotriazole (HOBt), N,N-diisopropylcarbodiimide (DIC), hexafluorophosphate benzotriazole tetramethyluronium (HBTU), N,N-diisopropylethylamine (DIPEA), benzotriazol-1-yl-oxy-tris(dimethyl-amino)-phosphonium hexafluorophosphate (BOP), and O-(7-azabenzotriazol-1-yl)-1,1,3,3-tetramethyluronium hexafluorophosphate (HATU).

4. 2. The process of claim 1, wherein the solvent for the coupling reaction is selected from dimethylformamide (DMF), pyridine, acetic anhydride, methanol, ethanol, isopropanol, dichloroethane, 1,4-dioxane, 2-methyltetrahydrofuran, N-methyl-2-pyrrolidinone (NMP), ethyl acetate, acetonitrile, and acetone.

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