Composition for growing bacteria of the genus Faecalis, composition for increasing butyric acid production, and composition for improving intestinal environment

Abstract

To provide: a composition for proliferating faecalibacterium genus bacterium exhibiting an excellent proliferating action of the faecalibacterium genus bacterium; a composition for increasing butyric acid production exhibiting an excellent butyric acid production increasing action; and a composition for improving an intestinal environment exhibiting an excellent intestinal environment improving action.SOLUTION: The composition for proliferating faecalibacterium genus bacterium includes at least one selected from a Brassicaceae genus vegetable and a pumpkin genus fruit vegetable; it may be constituted as a beverage composition containing at least one extract, squeezing liquid, crushed product or ground product selected from the Brassicaceae genus vegetable and the pumpkin genus fruit vegetable; the composition for increasing butyric acid production contains the composition for proliferating faecalibacterium genus bacterium; and the composition for improving an intestinal environment contains the composition for proliferating faecalibacterium genus bacterium.SELECTED DRAWING: None

Description

[Technical Field]

[0001] The present invention relates to a composition for growing bacteria of the genus Faecalibacterium, a composition for increasing butyric acid production, and a composition for improving the intestinal environment. [Background technology]

[0002] It is becoming clear that the balance of bacterial species in the intestinal flora (bacterial flora) plays an important role in maintaining health. Although the intestinal flora is a relatively stable ecosystem, its composition and activity can change due to various factors. If the balance of bacterial species in the intestinal flora is disrupted due to stress, aging, etc., it may affect health.

[0003] The enterobacterial genus Faecalibacterium is known to be an obligate anaerobic bacillus that resides primarily in the mucus layer secreted on the colonic epithelial mucosa and produces short-chain fatty acids. For example, Faecalibacterium prausnitzii is said to account for approximately 5-15% of the intestinal flora. It supplies short-chain fatty acids such as butyrate, which is the main energy source for colonic epithelial cells, and has beneficial effects on the body, such as anti-inflammatory properties, and is therefore attracting attention as a next-generation probiotic.

[0004] Conventionally, efforts have been made to grow bacteria of the genus Faecalibacterium in the intestines. For example, Patent Document 1 discloses an agent for growing bacteria of the genus Faecalibacterium, which contains 1-kestose as an active ingredient. [Prior art documents] [Patent documents]

[0005] [Patent Document 1] International Publication No. 2017 / 159647 Summary of the Invention [Problem to be solved by the invention]

[0006] An object of the present invention is to provide a composition for growing bacteria of the genus Faecalibacterium, which exhibits an excellent effect of growing bacteria of the genus Faecalibacterium. Another object of the present invention is to provide a composition for increasing butyric acid production, which exhibits an excellent effect of increasing butyric acid production. Another object of the present invention is to provide a composition for improving the intestinal environment, which exhibits an excellent effect of improving the intestinal environment. [Means for solving the problem]

[0007] The present invention is based on the discovery that Brassica vegetables or Cucurbit fruit vegetables can promote the growth of Faecalis bacterium. To achieve the above object, the composition for growing bacteria of the genus Faecalis of the present invention is characterized by containing at least one selected from Brassica vegetables and Cucurbit fruit vegetables.

[0008] The composition for growing Faecalibacterium bacteria may be configured as a beverage composition containing an extract, squeezed liquid, crushed product, or ground product of at least one kind selected from Brassica vegetables and Cucurbita fruit vegetables.

[0009] The composition for growing bacteria of the genus Faecalibacterium may also promote the production of short-chain fatty acids. The Faecalibacterium bacterium may be Faecalibacterium prausnitzii.

[0010] In the composition for growing Faecalibacterium bacteria, the at least one selected from Brassica vegetables and Cucurbita fruit vegetables may be at least one selected from cabbage, broccoli, and pumpkin.

[0011] In the composition for growing Faecalibacterium bacteria, the at least one selected from Brassica vegetables and Cucurbita fruit vegetables may be cabbage, broccoli, and pumpkin.

[0012] In the composition for growing Faecalibacterium bacteria, the at least one selected from Brassica vegetables and Cucurbita fruit vegetables may be broccoli and pumpkin.

[0013] The composition for growing Faecalibacterium bacteria may contain the Brassica vegetables and at least one selected from Cucurbita fruit vegetables, Malus fruit, Amaranthaceae vegetables, Umbelliferae root vegetables, Lamiaceae vegetables, Solanaceae fruit vegetables, Amaryllidaceae vegetables, and dietary fiber.

[0014] To achieve the above object, the composition for increasing butyric acid production of the present invention is characterized by including the composition for growing bacteria of the genus Faecalis. In order to achieve the above object, the composition for improving the intestinal environment of the present invention is characterized by containing the composition for growing bacteria of the genus Faecalis. [Effects of the Invention]

[0015] According to the present invention, an excellent effect of increasing the proliferation of bacteria of the genus Faecalibacterium can be exhibited.Furthermore, according to the present invention, an excellent effect of increasing butyric acid production can be exhibited.Furthermore, according to the present invention, an excellent effect of improving the intestinal environment can be exhibited. DETAILED DESCRIPTION OF THE INVENTION

[0016] First Embodiment A first embodiment of the composition for growing Faecalibacterium bacteria (hereinafter referred to as the "growing composition") of the present invention will be described. The component contained in the growing composition of this embodiment is at least one selected from Brassica vegetables and Cucurbita fruit vegetables. Specific examples of Brassica vegetables include cabbage, broccoli, cauliflower, rape, turnip, kale, Chinese cabbage, bok choy, and komatsuna. Among these, Brassica oleracea species such as cabbage, broccoli, cauliflower, and kale are preferred, with cabbage and broccoli being more preferred, due to their excellent ability to grow Faecalibacterium bacteria. The part of the Brassica vegetable is not particularly limited, and may be any of the flowers, flower buds, stems, leaves, and roots. The type of Cucurbita is not particularly limited, as long as it is an edible fruit vegetable. These plants may be used alone or in combination of two or more. From the viewpoint of excellent proliferation activity of Faecalibacterium bacteria, a combination of cabbage, broccoli, and pumpkin, or a combination of broccoli and pumpkin is preferable. When a combination of cabbage, broccoli, and pumpkin is used, the blending ratio thereof is appropriately set, for example, the mass ratio of the ingredients is 1:0.5-5:0.1-1. When a combination of broccoli and pumpkin is used, the blending ratio thereof is appropriately set, for example, the mass ratio of the ingredients is 1:0.1-5.

[0017] The propagation composition preferably contains Brassica vegetables and at least one selected from Cucurbita fruit vegetables, Malus fruit, Amaranthaceae vegetables, Umbelliferae root vegetables, Lamiaceae vegetables, Solanaceae fruit vegetables, Amaryllidaceae vegetables, and dietary fiber. The synergistic effect of Brassica vegetables and Cucurbita fruit vegetables further enhances the proliferation of Faecalibacterium bacteria. Specific examples of Amaranthaceae vegetables include spinach and okahijiki. Specific examples of Amaranthaceae root vegetables include carrots. Specific examples of Lamiaceae vegetables include perilla, mint, and herbs. Specific examples of Solanaceae fruit vegetables include tomatoes, eggplants, chili peppers, bell peppers, paprika, habanero peppers, and shishito peppers. Specific examples of Amaryllidaceae vegetables include onions, chives, garlic, leeks, and rakkyo. These plants may be used alone or in combination of two or more. The blending ratio of Brassica vegetables to the above-mentioned Cucurbit fruit vegetables and the like is set appropriately, and may be, for example, 1:0.1-5 in terms of the mass ratio of the ingredients.

[0018] The dietary fiber may be either water-soluble or insoluble. Specific examples of water-soluble dietary fiber include pectin, guar gum, glucomannan, β-glucan, polydextrose, inulin, gum arabic, indigestible dextrin, indigestible oligosaccharides, agarose, sodium alginate, carrageenan, and fucoidan. Specific examples of indigestible oligosaccharides include fructooligosaccharides and galactooligosaccharides. Specific examples of insoluble dietary fiber include cellulose, chitin, and chitosan. These dietary fibers may be used alone or in combination of two or more.

[0019] The form of the above-mentioned vegetables, fruit vegetables, root vegetables, or fruits is not particularly limited and is appropriately selected depending on the form of ingestion. Examples include liquid, solid, diced, crushed, ground, etc. Examples of liquid forms include extracts and squeezed liquids, which may be subjected to treatments such as dilution, concentration, and separation of insoluble components as necessary. As the solvent for the extract, preferably water or a hydrophilic organic solvent such as ethanol is used. Examples of solid forms include powders and granules. Examples of crushed or crushed products include pastes and purees. Among these, taking into consideration ease of ingestion, digestibility, etc., it is preferable to prepare the above-mentioned material as an extract, squeezed liquid, crushed, or ground product. The above-mentioned material may be raw or may be cooked, for example, by heating.

[0020] The application form of the growth composition is not particularly limited, and it can be applied as, for example, a food composition, a pharmaceutical composition, a quasi-drug composition, etc. The growth composition may be made by using the above-mentioned processed material as it is as a material for a food composition, a pharmaceutical composition, etc., or by appropriately blending it with a base, carrier, additive, etc. that is pharmacologically or food hygienically acceptable, to form a product.

[0021] When applied to food compositions such as foods and beverages, the proliferation composition can be used in various beverages, food additive compositions, various confectioneries (baked goods such as wafers and biscuits, candies, tablets, etc.), tablets, capsules (soft capsules and hard capsules), breads, various processed foods, jelly foods (jelly, agar, jelly drinks, etc.), soups, tablets to be dissolved in water and consumed, etc. The beverages can be applied to soft drinks as well as alcoholic and non-alcoholic beverages. Specific examples of beverages include vegetable juice drinks, vegetable and fruit juice drinks, sports drinks, vinegar drinks, energy drinks, wine, plum wine, various carbonated beverages, and powdered drinks (powdered vegetable drinks, etc.). The type of fruit juice to be combined is not particularly limited, and examples include juices derived from citrus fruits such as lemon, grapefruit, mandarin orange, and lime, as well as apple, pineapple, prune, grape, blueberry, peach, acai, kiwi, strawberry, mango, and plum. Among these, vegetable juice drinks and vegetable and fruit juice drinks are preferred from the viewpoint of efficiently ingesting the above-mentioned ingredients. The container in which the drink is filled may be any commonly used container, such as a can, a bottle, a plastic container such as a PET (polyethylene terephthalate) bottle, or a paper container. By storing the drink in this manner, it is possible to obtain a drink that is prevented from denaturing or deteriorating over the long term.

[0022] The uses of food compositions are not particularly limited and include so-called (1) general foods, (2) health foods such as nutritional supplements, health supplements, nutritionally balanced foods, and supplements, (3) health functional foods such as foods for specified health uses, nutrient-functional foods, foods with functional claims, and functional foods, and (4) foods for special dietary uses such as foods for sick people and infant foods. When a use indication is to be attached to a food or beverage, examples include indications stipulated by various laws, enforcement regulations, guidelines, etc. In the case of a food or beverage, use indications include indications on packaging such as wrapping and containers, as well as indications on advertising media such as pamphlets.

[0023] The contents of the labeling of the uses of the proliferation composition include the proliferation, increase, maintenance, and inhibition of the decline of intestinal Faecalibacterium bacteria, as well as suggestions thereof. Examples of such suggestions include the production or increase of short-chain fatty acids such as butyric acid produced in association with the proliferation of intestinal Faecalibacterium bacteria, and the improvement of the intestinal environment due to the production or increase of short-chain fatty acids. Examples of short-chain fatty acids include acetic acid, propionic acid, butyric acid, isobutyric acid, valeric acid, isovaleric acid, and caproic acid.

[0024] When the proliferation composition is used as a pharmaceutical composition or quasi-drug, it can be administered by oral ingestion or enteral administration. The dosage form is not particularly limited, and may be, for example, a tablet, pill, powder, liquid, suppository, suspension, emulsion, granule, powder, capsule, etc. When used as a pharmaceutical composition or quasi-drug, pharmaceutically acceptable bases, carriers, and additives include, for example, solvents, dispersants, emulsifiers, buffers, stabilizers, excipients, binders, disintegrants, lubricants, etc.

[0025] Next, the action of the proliferation composition of this embodiment will be described. Ingestion of the growth composition promotes the growth of Faecalibacterium bacteria in the intestine. The genus Faecalibacterium is primarily present in the mucus layer secreted on the large intestine epithelial mucosa and is known as a butyrate-producing bacterium or short-chain fatty acid-producing bacterium that produces mainly butyric acid as a short-chain fatty acid. The type of Faecalibacterium is not particularly limited as long as it is an intestinal bacterium, and examples include Faecalibacterium prausnitzii. As Faecalibacterium bacteria grow in the intestine, the production of short-chain fatty acids is also promoted.

[0026] The effects of the proliferation composition of this embodiment will be described. (1-1) The propagation composition of this embodiment contains at least one selected from Brassica vegetables and Cucurbita fruit vegetables. Therefore, ingestion of the propagation composition exerts an excellent effect of promoting the proliferation of Faecalibacterium bacteria, such as Faecalibacterium prausnitzii. Faecalibacterium bacteria, as so-called beneficial bacteria, contribute to maintaining good health by maintaining an acidic intestinal environment and suppressing the proliferation of harmful bacteria.

[0027] (1-2) When the composition for propagation is configured as a beverage composition containing an extract, squeezed liquid, crushed product, or ground product of at least one selected from Brassica vegetables and Cucurbita fruit vegetables, it can be easily ingested and is highly digestible, so that a more effective proliferation-promoting effect of Faecalibacterium bacteria can be expected.

[0028] (1-3) Ingestion of the proliferation composition promotes the proliferation of Faecalibacterium bacteria in the intestine. As proliferation in the intestine occurs, the production of short-chain fatty acids is also promoted. Short-chain fatty acids, such as butyric acid, epigenetically induce the expression of the Foxp3 gene in naive T cells in the lamina propria of the colon, inducing their differentiation into regulatory T cells (Treg). Tregs act to suppress excessive inflammatory responses and suppress the onset of allergies, atopy, and other conditions, and are therefore expected to regulate intestinal immunity. Furthermore, butyric acid is also a major energy source for colonic epithelial cells, and is expected to play a role in maintaining colonic function.

[0029] (1-4) When the propagation composition contains Brassica vegetables and at least one selected from Cucurbitaceae fruit vegetables, Malus fruit vegetables, Amaranthaceae vegetables, Umbelliferae root vegetables, Lamiaceae vegetables, Solanaceae fruit vegetables, Amaryllidaceae vegetables, and dietary fiber, the synergistic effect of these vegetables etc. further improves the proliferation of Faecalibacterium bacteria, thereby further improving the efficacy brought about by the proliferation of Faecalibacterium bacteria.

[0030] The above embodiment can be modified as follows: This embodiment and the following modifications can be combined with each other within the scope of technical compatibility. The growth composition of the above embodiment exhibits an excellent effect of increasing butyric acid production by promoting the growth or metabolism of bacteria of the genus Faecalibacterium. Therefore, the growth composition may be configured as a composition for increasing butyric acid production, which includes the growth composition.

[0031] The daily intake amount and intake period of the growth composition of the above embodiment can be appropriately set depending on the purpose, condition, etc. From the viewpoint of achieving excellent growth activity of Faecalibacterium bacteria, an example of an intake amount based on the content of Brassica vegetables and Cucurbit fruit vegetables per day is 0.1 to 500 g, of which 1 to 350 g is preferred, and 1 to 160 g is more preferred.

[0032] The proliferation composition of the above embodiment may be taken once or multiple times a day. From the viewpoint of improving the intestinal environment, it is preferable to take it continuously for, for example, one week or more.

[0033] It may also be used to improve the intestinal environment of pets, livestock, and other domestic animals other than humans. Second Embodiment A second embodiment of the intestinal environment-improving composition of the present invention will be described. The second embodiment has the same configuration as the proliferation composition of the first embodiment, except as described below. This intestinal environment-improving composition contains the proliferation composition of the genus Faecalis bacteria of the first embodiment.

[0034] The composition for improving the intestinal environment exerts an excellent effect of improving the intestinal environment when taken orally. In addition to improving the intestinal environment, it may also be used to maintain or prevent the deterioration of the intestinal environment. Therefore, similar to the first embodiment, it is applied to the use of food compositions, pharmaceutical compositions, and quasi-drugs. Furthermore, when labeling the use of food and drink compositions, examples include labeling stipulated by various laws, enforcement regulations, guidelines, etc. In the case of foods and drinks, labeling the use includes labeling on packaging such as wrapping and containers, as well as labeling on advertising media such as pamphlets.

[0035] The content of the indication of use includes indications of improvement of the intestinal environment, as well as indications of maintaining the intestinal environment or preventing its deterioration as described above. It also includes indications suggesting these improvements, maintenance, or prevention of deterioration. Examples include indications of improving the intestinal flora, improving bowel movements, improving constipation, and improving stool hardness. More specifically, examples of indications include "improving the intestinal environment and improving bowel movements," "improving intestinal flora and improving bowel movements," "food suitable for people prone to constipation," "regulating the stomach," "softening or smoothing hard stool," "increasing beneficial bacteria such as butyric acid-producing bacteria in the intestines, improving the intestinal environment or improving bowel movements," and "increasing the frequency or amount of bowel movements."

[0036] According to the composition for improving intestinal environment of this embodiment, the following effects can be obtained in addition to the effects of the first embodiment. (2-1) The intestinal environment-improving composition of this embodiment contains at least one selected from Brassica vegetables and Cucurbita fruit vegetables. Therefore, oral ingestion of the intestinal environment-improving composition exerts an excellent effect of improving the intestinal environment. Specifically, it exerts effects such as improving intestinal flora, bowel movements, constipation, and stool hardness. [Example]

[0037] Next, the above-described embodiment will be described in more detail with reference to examples and comparative examples. Note that the present invention is not limited to the configurations described in the examples section. <Test Example 1: Bacterial Growth Test 1> Various beverage ingredients were prepared and tested for the growth of Faecalibacterium bacteria.

[0038] (Material preparation) The materials evaluated for each example are shown in Table 1 below. Concentrated juice or puree was used as the material, and in the case of concentrated juice, it was adjusted to have the same Brix as straight juice. Both puree and concentrated juice were centrifuged, and the supernatant was sterilized with a 0.22 μm filter before use. For each formulation in Examples 4 and 5, the raw materials (concentrated juice or puree) were mixed and sterilized, and then filled into a paper container (Cartocan: manufactured by Toppan Packaging Services Co., Ltd.). The form of each material in Examples 4 and 5 was the same as that used in Examples 1 to 3 and Comparative Examples 1 and 2.

[0039] [Table 1]

[0040] [Table 2] (Bacterial culture method, growth test, and butyric acid concentration measurement) The Faecalibacterium bacterium used was Faecalibacterium prausnitzii ATCC (American Type Culture Collection) 27768. ATCC medium (2107 Modified Reinforced Clostridial Medium) was used as the culture medium. After adding each of the above samples and culturing, the growth of F. prausnitzii and the amount of butyric acid produced in the medium were measured (n = 3).

[0041] (1) First, the above bacteria were cultured in ATCC medium until the turbidity (660 nm) reached 1 or more. (2) Test media were prepared by adding the supernatant of each material to ATCC medium to a concentration of 37.5% by mass.

[0042] (3) The test medium was inoculated with the bacterial solution prepared in (1) at 5% by mass. The test medium was then anaerobically cultured at 37°C for 28 hours, and the turbidity of the culture solution was measured. As a control, purified water was added instead of the sample solution, and the turbidity of the culture solution was measured.

[0043] The results are shown in Table 3 (*: P<0.05 versus control by one-way ANOVA with Dunnett T3). (4) The culture medium was centrifuged, and the butyric acid concentration was measured using the supernatant. The sample was diluted 50 times, and the butyric acid concentration was measured by ion chromatography.

[0044] The results are shown in Table 3 (*: P<0.05 versus control by one-way ANOVA with Dunnett T3).

[0045] [Table 3] As shown in Table 3, it was confirmed that the OD660 of each example after 28 hours of culture was significantly increased compared to the control.

[0046] As shown in Table 3, it was confirmed that the butyric acid concentration after 28 hours of culture in each example was significantly increased compared to the control. The results in Table 3 show that there is a strong correlation between the proliferation of Faecalibacterium bacteria and the amount of butyric acid produced, and it is thought that the butyric acid concentration increases as the number of bacteria increases.

[0047] Drinking beverages containing these vegetables or fruits promotes the proliferation of Faecalibacterium bacteria and the production of short-chain fatty acids, maintaining an acidic intestinal environment. This is expected to suppress the proliferation of harmful bacteria and improve intestinal immunity by promoting Treg differentiation.

[0048] (Consideration) Using the results of the growth and butyric acid production of Faecalibacterium bacteria in each ingredient (Examples 1 to 3 and Comparative Examples 1 and 2) except for the fruit juice for adjusting acidity, the theoretical bacterial growth and butyric acid production were calculated from the amount of each ingredient used in the formulated beverages of Examples 4 and 5. The results were compared with the actual measured values ​​of Examples 4 and 5. The results are shown in Table 4 (*: P<0.05 two-tailed test).

[0049] [Table 4] As shown in Table 4, Example 4 showed significant bacterial growth of about 1.8 times the theoretical value. Furthermore, a significant increase in butyric acid production of about 2.3 times the theoretical value was observed. Example 5 showed significant bacterial growth of about 3.4 times the theoretical value. Furthermore, a significant increase in butyric acid production of about 2.5 times the theoretical value was observed. Therefore, combining the ingredients demonstrated a synergistic effect on bacterial growth and the associated promotion of short-chain fatty acid production.

[0050] <Test Example 2: Bacterial Growth Test 2> Various beverage ingredients were prepared using the ingredients in each example shown in Table 5 below, and the growth of Faecalibacterium bacteria in each ingredient alone was tested. The preparation method for each ingredient was the same as in Test Example 1. Dietary fiber was prepared as a 200 mg / mL aqueous solution of each ingredient.

[0051] The growth test for Faecalibacterium bacteria was performed in the same manner as in Test Example 1, except that the total of the ATCC medium and bacterial solution was 25% by mass, the concentration of the supernatant of each material was 18.75% by mass or 37.5% by mass, and the remainder was water. Dietary fiber was added to the ATCC medium as each prepared aqueous solution at 18.75% by mass or 37.5% by mass. As a control, purified water was added instead of each material, and the turbidity of the culture solution was measured. The turbidity results for each example are shown in Table 5. "-" in the table indicates that no evaluation was performed.

[0052] [Table 5] As shown in Table 5, it was confirmed that the OD660 of each Example after 28 hours of culture was significantly increased compared to the control. On the other hand, it was confirmed that the OD660 of each Comparative Example material was significantly lower than that of each Example.

[0053] <Test Example 3: Bacterial Growth Test 3> Broccoli was used as a raw material in combination with each of the ingredients shown in Tables 6 and 7 below, and the proliferation of bacteria of the genus Faecalibacterium was tested. The preparation method for each ingredient was the same as in Test Example 2.

[0054] The growth test for Faecalibacterium bacteria was carried out in the same manner as in Test Example 2, except that the ATCC medium contained broccoli supernatant at a concentration of 9.375% by mass, and the supernatant of each material shown in Table 6 at a concentration of 9.375% by mass. The turbidity results for each example are shown in the "Co-culture" column of Table 6.

[0055] Furthermore, a growth test of Faecalibacterium bacteria was performed in the same manner as in Test Example 2, except that the broccoli supernatant was added to the ATCC medium at a concentration of 18.75% by mass, and the supernatant of each material shown in Table 7 was added at a concentration of 18.75% by mass. The turbidity results for each example are shown in the "Co-culture" column of Table 7.

[0056] In addition, the theoretical bacterial growth amount (turbidity) was calculated from the monoculture bacterial growth values ​​of each material shown in Table 5 and the amount of material used in each example in Tables 6 and 7, and is shown in the ``Theoretical Value'' column of Tables 6 and 7.

[0057] [Table 6]

[0058] [Table 7] As shown in Tables 6 and 7, a synergistic effect was observed in the growth of bacteria when broccoli was cultured in a medium containing Cucurbita genus fruit vegetables, Malus genus fruit vegetables, Amaranthaceae vegetables, Umbelliferae root vegetables, Lamiaceae vegetables, Solanaceae fruit vegetables, Amaryllidaceae vegetables, or dietary fiber.

[0059] Similar co-culture tests were also conducted using broccoli and watercress or komatsuna, which are Brassica vegetables, but no synergistic effect on bacterial growth was observed (data not attached). Also, similar co-culture tests were conducted using broccoli and parsley, a leafy vegetable in the Apiaceae family, but no synergistic effect on bacterial growth was observed (data not attached).

[0060] <Test Example 4: Human intestinal environment improvement test> The beverage prepared in Example 4 was used to test whether continuous consumption would improve the intestinal environment.

[0061] 381 children under the age of three were asked to take one bottle (125g) per day for three days to two weeks. After the intake period, they were asked to answer the questions shown in Table 8 below, and the percentage of people who saw an improvement was calculated.

[0062] [Table 8] As shown in Table 8, improvements were observed especially in bowel movements and softness of stools. The percentage of people who answered that they met any of these questions was 45% of the total.

[0063] Furthermore, of the 381 children who took the supplement (79 in total) who had been suffering from constipation before taking it, 49% reported that their bowel movements had improved. Similarly, 32% reported that their stools had become softer.

[0064] <Example of prescription> Vegetable and fruit juice drinks and powdered drinks were prepared by mixing the ingredients shown in Tables 9 and 10 below.

[0065] [Table 9]

[0066] Table 10

Claims

1. At least one selected from Brassica vegetables and Cucurbit fruit vegetables, The Brassica vegetable is at least one selected from cabbage and broccoli, At least one selected from the Brassica vegetables and Cucurbita fruit vegetables is blended as at least one selected from a squeezed liquid, a crushed product, a ground product, a concentrated juice, a concentrated reconstituted liquid, and a puree; A composition for growing bacteria of the genus Faecalis in the human intestine, characterized in that the daily intake amount for humans is 1 to 500 g based on the content of the Brassica vegetables and Cucurbit fruit vegetables.

2. The composition for growing bacteria of the genus Faecalis according to claim 1, wherein the composition is configured as a beverage composition.

3. A composition for growing Faecalibacterium bacteria as described in claim 1 or 2, which promotes the production of short-chain fatty acids.

4. A composition for growing Faecalibacterium bacteria described in any one of claims 1 to 3, wherein the Faecalibacterium bacteria is Faecalibacterium prausnitzii.

5. A composition for growing bacteria of the genus Faecalis according to any one of claims 1 to 4, which contains cabbage, broccoli, and pumpkin.

6. A composition for growing bacteria of the genus Faecalis according to any one of claims 1 to 4, which contains broccoli and pumpkin.

7. A composition for growing Faecalis bacteria described in any one of claims 1 to 6, which contains the Brassica vegetable and at least one selected from Cucurbita fruit vegetables, Malus fruit, Amaranthaceae vegetables, Umbelliferae root vegetables, Lamiaceae vegetables, Solanaceae fruit vegetables, Amaryllidaceae vegetables, and dietary fiber.

8. A composition for increasing human intestinal butyric acid production, characterized by comprising a composition for growing bacteria of the genus Faecalis described in any one of claims 1 to 7.

9. A composition for improving the human intestinal environment, characterized by comprising a composition for growing bacteria of the genus Bacillus subtilis described in any one of claims 1 to 7.

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