Bottled beverage containing vitamin B12 and carnosine

Abstract

To provide a novel food and drink containing at least one cobalamin selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitecobalamin.SOLUTION: According to one aspect of the present invention, a bottled beverage is provided containing carnosine and at least one cobalamin selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitecobalamin, wherein the content of carnosine is 0.70 to 15 mg / mL, and the content of the at least one cobalamin is 0.005 to 1 μg / mL.SELECTED DRAWING: None

Description

[Technical Field]

[0001] This invention relates to vitamin B 12 This invention relates to a packaged beverage containing cobalamins, such as at least one cobalamin selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin, and carnosine, as well as a method for producing the same. The invention further relates to a method for stabilizing cobalamins and a method for improving the aftertaste of carnosine. The invention also relates to an inhibitor of cobalamin degradation. [Background technology]

[0002] In recent years, due to the growing health consciousness, various foods and beverages containing a wide range of nutrients are being manufactured and sold. Examples include foods and beverages containing various vitamins and minerals. A variety of vitamins are added to foods and beverages, with vitamin A, B vitamins, vitamin C, vitamin E, and vitamin K being common examples. B vitamins include vitamin B1, vitamin B2, vitamin B6, and vitamin B1. 12 Niacin, pantothenic acid, folic acid, and biotin are commonly used.

[0003] Each vitamin has its own known nutritional function. According to the website of the Consumer Affairs Agency of Japan, vitamin A is listed as a nutrient that helps maintain the health of the skin and mucous membranes, vitamin B1 is listed as a nutrient that helps produce energy from carbohydrates and maintain the health of the skin and mucous membranes, and vitamin B 12 It is listed as a nutrient that helps in the formation of red blood cells.

[0004] On the other hand, some vitamins are easily broken down, and much research is being conducted on how to stabilize these vitamins in food and beverages. For example, vitamin B 12 Heat and other components in food and beverages can destabilize it and accelerate its decomposition.

[0005] Japanese Patent Application Laid-Open No. 2019-110827 (Patent Document 1) describes that in an acidic composition, the decomposition rate of vitamin B 12 increases and / or its stability decreases due to the presence of ethanol, and an acidic composition containing vitamin B 12 characterized by a reduced ethanol content has been proposed. Japanese Patent Application Laid-Open No. 2022-162843 (Patent Document 2) describes that vitamin B 12 decomposes in an acidic aqueous solution, and a stock solution for a water-soluble vitamin drink has been proposed, which contains (1) a water-soluble vitamin, (2) one or more selected from polyoxyethylene hydrogenated castor oil and polyoxyethylene castor oil, (3) one or more selected from the group consisting of polyglycerol fatty acid esters, polyoxyethylene sorbitan fatty acid esters, polyoxyethylene glycerol fatty acid esters, sucrose fatty acid esters, and polyoxyethylene alkyl ethers, and (4) a polyhydric alcohol. Japanese Patent Application Laid-Open No. 2023-542821 (Patent Document 3) describes that vitamin B 12 suffers significant losses upon heating, and a preparation of vitamin B 12 essentially free of glucose, amino acids, vitamin B1, ascorbyl palmitate, and / or vitamin C has been proposed.

Prior Art Documents

Patent Documents

[0006]

Patent Document 1

Patent Document 2

Patent Document 3

Summary of the Invention

Problems to be Solved by the Invention

[0007] Under the above circumstances, there is a need to develop a new food or drink containing at least one cobalamin selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin.

Means for Solving the Problems

[0008] As a result of investigations by the present inventors, it was found that decomposition of cobalamin can be suppressed by including a predetermined amount of carnosine in a container-packed beverage containing a predetermined amount of cobalamin. Further, as a result of investigations by the present inventors, it was also found that cobalamin can improve the undesirable taste properties of carnosine. The present invention is based on such findings.

[0009] The present invention includes, for example, inventions of the following aspects. [1] A container-packed beverage containing carnosine and at least one cobalamin selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin, wherein the content of carnosine is 0.70 to 15 mg / mL, and the content of the at least one cobalamin is 0.005 to 1 μg / mL. [2] The container-packed beverage according to [1], which contains chicken extract. [3] The container-packed beverage according to [1] or [2], wherein the at least one cobalamin includes cyanocobalamin. [4] The container-packed beverage according to any one of [1] to [3], wherein the content of carnosine is 0.75 to 7 mg / mL. [5] The container-packed beverage according to any one of [1] to [4], wherein the content of carnosine is 0.80 to 4 mg / mL. [6] A packaged beverage according to any one of [1] to [5], wherein the content of at least one of the aforementioned cobalamins is 0.04 to 0.4 μg / mL. [7] A packaged beverage according to any one of [1] to [6], wherein the weight ratio of the carnosine content to the content of at least one of the cobalamins is 500:1 to 50000:1. [8] A packaged beverage according to any one of [1] to [7], wherein, after a retort test at 120°C for 8 minutes, the amount of at least one cobalamin is maintained at 80% by weight or more compared to the amount of at least one cobalamin before the retort test. [9] A packaged beverage as described in any of [1] to [8], which is a ready-to-drink (RTD) beverage, a soft drink, chicken soup, a jelly beverage, or a functional beverage.

[10] A method for producing a packaged beverage as described in any of [1] to [9], A method comprising adding at least one of the aforementioned cobalamin and at least one of carnosine to a beverage base.

[11] A method for stabilizing at least one cobalamin selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin, A method comprising adding carnosine to a solution containing at least one of the aforementioned cobalamins at a concentration of 0.01 to 15 mg / mL.

[12] A method to improve the aftertaste of carnosine, In a solution containing 0.01 to 15 mg / mL of carnosine, A method comprising containing at least one cobalamin selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin, to a concentration of 0.005 to 1 μg / mL. [3] Carnosine and, At least one cobalamin selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin A packaged beverage containing, The carnosine content is 0.50-15 mg / mL. The content of at least one of the aforementioned cobalamins is 0.005 to 1 μg / mL, and A bottled beverage containing less than 0.3 mg / mL of propylene glycol.

[14] A packaged beverage as described in any one of items [1] to [9] and

[13] , having an ethanol content of less than 0.001 mg / mL.

[15] A cobalamin degradation inhibitor, comprising carnosine, selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin. [Effects of the Invention]

[0010] According to one aspect of the present invention, it is possible to provide a packaged beverage in which the stability of at least one cobalamin selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin is improved. According to another aspect of the present invention, it is possible to provide a packaged beverage containing at least one cobalamin selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin in which the undesirable taste quality of carnosine is improved. [Brief explanation of the drawing]

[0011] [Figure 1] Figure 1 is a graph showing the retention rate (%) of cyanocobalamin in each experimental sample, with the amount of cyanocobalamin in the control sample (without retort heat treatment) set to 100. [Figure 2]Figure 2 shows the average retention rate (%) for different carnosine concentrations, based on data obtained from experimental samples containing cyanocobalamin at concentrations of 0.005 μg / mL, 0.04 μg / mL, 0.05 μg / mL, 0.1 μg / mL, and 0.4 μg / mL. [Modes for carrying out the invention]

[0012] The present invention will now be described in detail. The following embodiments are illustrative for illustrating the present invention and are not intended to limit the invention to these embodiments only. The present invention can be carried out in various forms without departing from its spirit. Any paragraph headings used herein are for structural purposes only and should not be construed as limiting the subject matter described. Furthermore, all documents cited in this specification, as well as published gazettes, patent gazettes, and other patent documents, shall be incorporated herein by reference.

[0013] 1. Beverages containing cobalamin and carnosine In one aspect, the present invention provides a beverage containing cobalamin and carnosine (hereinafter also referred to as "the beverage of the present invention"). According to a first aspect of the present invention, Carnosine and, A packaged beverage comprising at least one cobalamin selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin, The carnosine content is 0.70-15 mg / mL. A packaged beverage is provided, having a cobalamin content of 0.005 to 1 μg / mL.

[0014] According to a second aspect of the present invention, Carnosine and, A packaged beverage comprising at least one cobalamin selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin, The carnosine content is 0.50-15 mg / mL. The content of at least one of the aforementioned cobalamins is 0.005 to 1 μg / mL. A bottled beverage containing less than 0.3 mg / mL of propylene glycol is offered.

[0015] The following describes specific embodiments of the first and second embodiments of the present invention. Unless otherwise specified, the embodiments described below fall under either the first or second embodiment.

[0016] Surprisingly, the inventors have found that the stability of cobalamin is improved by keeping the cobalamin content and the carnosine content within a predetermined range. Although we do not wish to be bound by theory, in the basic structure of cobalamin, the cobalt ion is coordinated to the dimethylbenzimidazole moiety, and this dimethylbenzimidazole is normally substituted with histidine which has an imidazole moiety. Similarly, it is presumed that carnosine, which also contains an imidazole moiety, chelates the cobalt ion, forming a stable carnosine-cobalt complex. As confirmed in the examples described later, the stability of cyanocobalamin (a type of cobalamin) tends to improve as the concentration of carnosine increases, but conversely, it has been observed that the stability of cyanocobalamin tends to decrease as the concentration of carnosine increases. While I don't want to be constrained by theory on this point either, it is presumed that if the concentration of carnosine becomes too high, the coordination of dimethylbenzimidazole will be lost, resulting in an unstable structure for cobalamin.

[0017] A beverage according to a preferred embodiment of the present invention can suppress the decomposition of the above-mentioned at least one cobalamin by heat sterilization treatment at high temperatures, such as in a retort test. In one embodiment of the present invention, after a retort test at 120°C for 8 minutes, the beverage maintains more than 70% by weight of the above-mentioned at least one cobalamin relative to the cobalamin content before the retort test. In a preferred embodiment of the present invention, after a retort test at 120°C for 8 minutes, the beverage maintains 75% or more by weight, 80% or more by weight, 85% or more by weight, 90% or more by weight, 92% or more by weight, 94% or more by weight, 96% or more by weight, 98% or more by weight, or about 100% by weight of the above-mentioned at least one cobalamin relative to the cobalamin content before the retort test.

[0018] The inventors also unexpectedly discovered that the undesirable taste characteristics of carnosine could be suppressed by keeping the content of carnosine and at least one of the above-mentioned cobalamins within a predetermined range. The mechanism of human taste is complex, and it was completely unexpected that the taste characteristics of carnosine were improved by the presence of at least one of the above-mentioned cobalamins in a very small amount.

[0019] <Cobalamin> Cobalamin is vitamin B 12 It is a general term for a group of vitamins and is classified as a water-soluble vitamin. Cobalamin is a cobalt complex. Among cobalamins, the most commonly used is cyanocobalamin, and its molecular formula is C 63 H 88 CoN 14 O 14 It is P. Its CAS number is 68-19-9. Other cobalamins besides cyanocobalamin include hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin. Hydroxocobalamin is a cobalamin that has a hydroxyl group instead of the cyano group of cyanocobalamin, and its molecular formula is C 62 H 89 CoN 13 O 15It is P and its CAS number is 13422-51-0. Adenosylcobalamin is a cobalamin that has an adenosyl group instead of a cyano group in cyanocobalamin, and its molecular formula is C 72 H 100 CoN 18 O 17 It is P and its CAS number is 13870-90-1. Methylcobalamin is a cobalamin that has a methyl group instead of a cyano group in cyanocobalamin, and its molecular formula is C 63 H 91 CoN 13 O 14 It is P and its CAS number is 13422-55-4. Sulfitocobalamin is a cobalamin that has a sulfito group instead of a cyano group in cyanocobalamin, and its molecular formula is C 62 H 89 CoN 13 O 17 It is PS and its CAS number is 15671-27-9. A beverage according to one aspect of the present invention contains at least one cobalamin selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin. In one aspect of the present invention, the above at least one cobalamin preferably includes cyanocobalamin and / or hydroxocobalamin, and more preferably includes cyanocobalamin.

[0020] The origin of the at least one cobalamin used in the present invention is not particularly limited. Therefore, in the present invention, chemically synthesized cobalamin may be used, biosynthesized from bacterial culture medium may be used, or cobalamin may be isolated from natural raw materials containing cobalamin. Alternatively, cobalamin may be added by adding cobalamin-containing food ingredients to a beverage. Food ingredients rich in cobalamin include seafood (especially clams and mussels) and animal meat (for example, chicken liver).

[0021] According to one aspect of the present invention, the content of at least one of the above-mentioned cobalamins in the beverage is 0.005 to 1 μg / mL. The content of at least one of the above-mentioned cobalamins is, for example, 0.01 to 1 μg / mL, 0.02 to 1 μg / mL, 0.03 to 1 μg / mL, 0.04 to 1 μg / mL, 0.05 to 1 μg / mL, 0.06 to 1 μg / mL, 0.07 to 1 μg / mL, 0.08 to 1 μg / mL, 0.09 to 1 μg / mL, 0.1 to 1 μg / mL, 0.15 to 1 μg / mL, 0.2 to 1 μg / mL, 0.25 to 1 μg / mL, 0.3 to 1 μg / mL, 0.35 to 1 μg / mL, 0.4 to 1 μg / mL, 0.005 to 0.8 μg / mL, 0.01 to 0.8 μg / mL, 0.02 to 0.8 μg / mL, 0.03 to 0.8 μg / mL, 0.04 to 0.8 μg / mL, 0.05 to 0.8 μg / mL, 0.06 to 0.8 μg / mL, 0.07 to 0.8 μg / mL, 0.08 to 0.8 μg / mL, 0.09 to 0.8 μg / mL, 0.1 to 0.8 μg / mL, 0.15 to 0.8 μg / mL, 0.2 to 0.8 μg / mL, 0.25 to 0.8 μg / mL, 0.3 to 0.8 μg / mL, 0.35 to 0.8 μg / mL, 0.4 to 0.8 μg / mL, 0.005 to 0.6 μg / mL, 0.01 to 0.6 μg / mL, 0.02 to 0.6 μg / mL, 0.03 to 0.6 μg / mL, 0.04 to 0.6 μg / mL, 0.05 to 0.6 μg / mL, 0.06 to 0.6 μg / mL, 0.07 to 0.6 μg / mL, 0.08 to 0.6 μg / mL, 0.09 to 0.6 μg / mL, 0.1 to 0.6 μg / mL, 0.15 to 0.6 μg / mL, 0.2 to 0.6 μg / mL, .....................4μg / mL, 0.02~0.4μg / mL, 0.03~0.4μg / mL, 0.04~0.4μg / mL, 0.05~0.4μg / mL, 0.06~0.4μg / mL, 0.07 ~0.4μg / mL, 0.08~0.4μg / mL, 0.09~0.4μg / mL, 0.1~0.4μg / mL, 0.15~0.4μg / mL, 0.2~0.4μg / mL, 0.25 ~0.4μg / mL, 0.3~0.4μg / mL, 0.35~0.4μg / mL, 0.005~0.2μg / mL, 0.01~0.2μg / mL, 0.02~0.2μg / mL, 0. 03~0.2μg / mL, 0.04~0.2μg / mL, 0.05~0.2μg / mL, 0.06~0.2μg / mL, 0.07~0.2μg / mL, 0.08~0.2μg / mL, 0.09~0.2μg / mL, 0.1~0.2μg / mL, 0.15~0.2μg / mL, 0.005~0.1μg / mL, 0.01~0.1μg / mL, 0.02~0.1μg / mL, 0.03~0.1μg / mL, 0.04~0.1μg / mL, 0.05~0.1μg / mL, 0.06~0.1μg / mL, 0.07~0.1μg / mL, 0.08~0.1μ The cobalamin content may be g / mL, 0.09-0.1 μg / mL, 0.005-0.09 μg / mL, 0.01-0.09 μg / mL, 0.02-0.09 μg / mL, 0.03-0.09 μg / mL, 0.04-0.09 μg / mL, 0.05-0.09 μg / mL, 0.06-0.09 μg / mL, 0.07-0.09 μg / mL, or 0.08-0.09 μg / mL. The content of at least one of the above cobalamins is preferably 0.02-0.4 μg / mL, more preferably 0.04-0.4 μg / mL. This content refers to the cobalamin content if the beverage contains only one type of cobalamin, and the total content if the beverage contains two or more types of cobalamins.

[0022] The content of cyanocobalamin in the beverage according to one aspect of the present invention may be 0.005 to 1 μg / mL, for example, 0.01 to 1 μg / mL, 0.02 to 1 μg / mL, 0.03 to 1 μg / mL, 0.04 to 1 μg / mL, 0.05 to 1 μg / mL, 0.06 to 1 μg / mL, 0.07 to 1 μg / mL, 0.08 to 1 μg / mL, 0.09 to 1 μg / mL, 0.1 to 1 μg / mL, 0.15 to 1 μg / mL, 0.2 to 1 μg / mL, 0.25 to 1 μg / mL, 0.3 to 1 μg / mL, 0.35 to 1 μg / mL, 0.4 to 1 μg / mL, 0.005 to 0.8 μg / mL, 0.01 to 0.8 μg / mL, 0.02 to 0.8 μg / mL, 0.03 to 0.8 μg / mL, 0.04 to 0.8 μg / mL, 0.05 to 0.8 μg / mL, 0.06 to 0.8 μg / mL, 0.07 to 0.8 μg / mL, 0.08 to 0.8 μg / mL, 0.09 to 0.8 μg / mL, 0.1 to 0.8 μg / mL, 0.15 to 0.8 μg / mL, 0.2 to 0.8 μg / mL, 0.25 to 0.8 μg / mL, 0.3 to 0.8 μg / mL, 0.35 to 0.8 μg / mL, 0.4 to 0.8 μg / mL, 0.005 to 0.6 μg / mL, 0.01 to 0.6 μg / mL, 0.02 to 0.6 μg / mL, 0.03 to 0.6 μg / mL, 0.04 to 0.6 μg / mL, 0.05 to 0.6 μg / mL, 0.06 to 0.6 μg / mL, 0.07 to 0.6 μg / mL, 0.08 to 0.6 μg / mL, 0.09 to 0.6 μg / mL, 0.1 to 0.6 μg / mL, 0.15 to 0.6 μg / mL, 0.2 to 0.6 μg / mL, 0.25 to 0.6 μg / mL, 0.3 to 0.6 μg / mL, 0.35 to 0.6 μg / mL, 0.4 to 0.6 μg / mL, 0.005 to 0.5 μg / mL, 0.01 to 0.5 μg / mL, 0.02 to 0.5 μg / mL, 0.03 to 0.5 μg / mL, 0.04 to 0.5 μg / mL, 0.05 to 0.5 μg / mL, 0.06 to 0.5 μg / mL, 0.07 to 0.5 μg / mL, 0.08 to 0.5 μg / mL, 0.09 to 0.5 μg / mL, 0.1 to 0.5 μg / mL, 0.15 to 0.5 μg / mL, 0.2 to 0.5 μg / mL, 0.25 to 0.5 μg / mL, 0.3 to 0.5 μg / mL, 0.35 to 0.5 μg / mL, 0.4 to 0.5 μg / mL, 0.005 to 0.4 μg / mL, 0.01 to 0.4 μg / mL, 0.02 to 0.4 μg / mL, 0.03 to 0.4μg / mL, 0.04~0.4μg / mL, 0.05~0.4μg / mL, 0.06~0.4μg / mL, 0.07~0.4μg / mL, 0.08~0.4μg / mL, 0 .09~0.4μg / mL, 0.1~0.4μg / mL, 0.15~0.4μg / mL, 0.2~0.4μg / mL, 0.25~0.4μg / mL, 0.3~0.4μg / m L, 0.35~0.4μg / mL, 0.005~0.2μg / mL, 0.01~0.2μg / mL, 0.02~0.2μg / mL, 0.03~0.2μg / mL, 0.04~ 0.2μg / mL, 0.05~0.2μg / mL, 0.06~0.2μg / mL, 0.07~0.2μg / mL, 0.08~0.2μg / mL, 0.09~0.2μg / mL, 0.1~0.2μg / mL, 0.15~0.2μg / mL, 0.005~0.1μg / mL, 0.01~0.1μg / mL, 0.02~0.1μg / mL, 0.03~0.1 μg / mL, 0.04~0.1μg / mL, 0.05~0.1μg / mL, 0.06~0.1μg / mL, 0.07~0.1μg / mL, 0.08~0.1μg / mL, 0. The cyanocobalamin content may be 0.09-0.1 μg / mL, 0.005-0.09 μg / mL, 0.01-0.09 μg / mL, 0.02-0.09 μg / mL, 0.03-0.09 μg / mL, 0.04-0.09 μg / mL, 0.05-0.09 μg / mL, 0.06-0.09 μg / mL, 0.07-0.09 μg / mL, or 0.08-0.09 μg / mL. The cyanocobalamin content is preferably 0.02-0.4 μg / mL, more preferably 0.04-0.4 μg / mL.

[0023] The content of at least one of the above-mentioned cobalamins can be determined by measuring with a liquid chromatography-mass spectrometer (LC-MS / MS), but if the content can be calculated from the amount used, it may be determined from the amount used. When measuring with LC-MS / MS, the measurement conditions can be measured using the standard measurement method of the analyzer. When measuring with LC-MS / MS, an AB Sciex 5600 and an Agilent 1290 binary LC equipped with a TripleTOF system can be used, and for example, a CORTECS T3 Column, 20 Å, 1.6 μm, 2.1 mm × 100 mm (manufactured by Waters Co., Ltd.) can be used as the LC column.

[0024] A packaged beverage according to one aspect of the present invention contains cyanocobalamin and at least one selected from the group consisting of hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin (hereinafter referred to as "other vitamin B"). 12 It includes other vitamin B (also called "types"). 12 If it contains other vitamins, check the amount of cyanocobalamin and other vitamin B 12 The composition ratio of the total amount of the class may be 99:1-90:10, 99:1-91:9, 99:1-92:8, 99:1-93:7, 99:1-94:6, 99:1-95:5, 99:1-96:4, 99:1-97:3, or 99:1-98:2 by mass ratio. A packaged beverage according to another aspect of the present invention contains vitamin B 12 It includes the vitamin B 12 The group consists substantially of cyanocobalamin. In this specification, "consisting essentially of" refers to the vitamin B used in the present invention. 12 Other types of vitamin B besides cyanocobalamin. 12 This means that it may contain small amounts of certain substances. For example, vitamin B 12 If the group consists substantially of cyanocobalamin, the cyanocobalamin content is vitamin B 12 This means that the total weight of the group is preferably 85-100% by weight, more preferably 90-100% by weight, and even more preferably 95-100% by weight. The cyanocobalamin content is the same as that of vitamin B12 100% by weight based on the total weight of the same type means that Vitamin B 12 The group consists of cyanocobalamin and any other vitamin B 12 This means that the content of the substance is less than 1% by weight (less than the amount of impurities).

[0025] Vitamin B 12 The health benefits of cobalamin include its hematopoietic and nerve repair effects. Therefore, the inclusion of cobalamin can be expected to improve eye strain, stiff shoulders, and neuralgia.

[0026] <Carnosine> A beverage according to one aspect of the present invention contains carnosine. Carnosine is a type of imidazole peptide, and imidazole dipeptides are a general term for dipeptides having histidine with an imidazole ring as a constituent component (Nobuya Yanai, Journal of the Japan Society for Food Chemistry and Technology, 2014, Vol. 61, No. 1, p. 45). Here, imidazole is a type of five-membered cyclic aromatic heterocyclic compound, and is a nitrogen-containing aromatic heterocyclic compound having nitrogen atoms at the 1st and 3rd positions.

[0027] The carnosine used in the beverage in one aspect of the present invention may be obtained by extraction from a carnosine-containing material (e.g., fish meat) with an aqueous solvent, or by chemical synthesis, and its origin and raw materials are not particularly limited. Alternatively, carnosine may be added to the beverage by adding a carnosine-containing food ingredient. For example, carnosine is abundant in fish meat such as tuna, bonito, and salmon, as well as beef, pork, and chicken. Mammals such as pigs and cattle contain a lot of carnosine, aquatic mammals have a carnosine to balenine ratio of about 1:2, birds have a carnosine to anserine ratio of 1:3, and large fish are said to contain almost only anserine (Nobuya Yanai, Journal of the Japan Society for Food Chemistry and Technology, 2014, Vol. 61, No. 1, p. 45). In a preferred aspect of the present invention, chicken extract, described later, can be used as a carnosine-containing food ingredient.

[0028] The carnosine content in a beverage in one embodiment of the first aspect of the present invention is 0.70 to 15 mg / mL. The carnosine content can be, for example, 0.75 to 15 mg / mL, 0.80 to 15 mg / mL, 0.90 to 15 mg / mL, 1 to 15 mg / mL, 1.5 to 15 mg / mL, 2 to 15 mg / mL, 2.5 to 15 mg / mL, 3 to 15 mg / mL, 3.5 to 15 mg / mL, 4 to 15 mg / mL, 4.5 to 15 mg / mL, 5 to 15 mg / mL, 5.5 to 15 mg / mL, 6 to 15 mg / mL, 6.5 to 15 mg / mL, 7 to 15 mg / mL, 7.5 to 15 mg / mL, 8 to 15 mg / mL, 0.70 to 12 mg / mL, 0.75 mg / mL. ~12mg / mL, 0.80~12mg / mL, 0.90~12mg / mL, 1~12mg / mL, 1.5~12mg / mL, 2~12mg / mL, 2.5~12mg / mL, 3~12mg / mL, 3.5~12mg / mL, 4~12mg / mL, 4.5~12 mg / mL, 5~12mg / mL, 5.5~12mg / mL, 6~12mg / mL, 6.5~12mg / mL, 7~12mg / mL, 7.5~12mg / mL, 8~12mg / mL, 0.70~10mg / mL, 0.75~10mg / mL, 0.80~10mg / mL, 0.90-10mg / mL, 1-10mg / mL, 1.5-10mg / mL, 2-10mg / mL, 2.5-10mg / mL, 3-10mg / mL, 3.5-10mg / mL, 4-10mg / mL, 4.5-10mg / mL, 5-10mg / mL, 5.5-10mg / mL, 6-10mg / mL, 6.5-10mg / mL, 7-10mg / mL, 7.5-10mg / mL, 8-10mg / mL, 0.70-8mg / mL, 0.75-8mg / mL, 0.80-8mg / mL, 0.90-8mg / mL, 1-8mg / mL, 1.5~8mg / mL, 2~8mg / mL, 2.5~8mg / mL, 3~8mg / mL, 3.5~8mg / mL, 4~8mg / mL, 4.5~8mg / mL, 5~8mg / mL, 5.5~8mg / mL, 6~8mg / mL, 6.5~8mg / mL, 7~8mg / mL, 7.5~8mg / mL, 0.70~7mg / mL, 0.75~7mg / mL, 0.80~7mg / mL, 0.90~7mg / mL, 1~7mg / mL, 1.5~7mg / mL, 2~7mg / mL, 2.5~7mg / mL, 3~7mg / mL, 3.5~7mg / mL, 4~7mg / mL, 4.5~7mg / mL, 5~7mg / mL, 5.5~7mg / mL, 6~7mg / mL, 6.5~7mg / mL, 0.70~6mg / mL, 0.75~6mg / mL, 0.80~6mg / mL, 0.90~6mg / mL, 1~6mg / mL, 1.5~6mg / mL, 2~6mg / mL, 2.5~6mg / mL, 3~6mg / mL, 3.5~6mg / mL, 4~6mg / mL, 4.5~6mg / mL, 5~6mg / mL, 5.5~6mg / mL, 0.70~4mg / mL, 0.75~4mg / mL, 0.80 The carnosine content may be ~4 mg / mL, 0.90~4 mg / mL, 1~4 mg / mL, 1.5~4 mg / mL, 2~4 mg / mL, 2.5~4 mg / mL, 3~4 mg / mL, 3.5~4 mg / mL, 0.70~3 mg / mL, 0.75~3 mg / mL, 0.80~3 mg / mL, 0.90~3 mg / mL, 1~3 mg / mL, 1.5~3 mg / mL, 2~3 mg / mL, 2.5~3 mg / mL, 0.70~2 mg / mL, 0.75~2 mg / mL, 0.80~2 mg / mL, 0.90~2 mg / mL, 1~2 mg / mL, or 1.5~2 mg / mL. The carnosine content is preferably 0.75~7 mg / mL, more preferably 0.80~4 mg / mL.

[0029] According to one aspect of a second aspect of the present invention, the carnosine content is 0.50 to 15 mg / mL. The carnosine content is, for example, 0.60 to 15 mg / mL, 0.70 to 15 mg / mL, 0.75 to 15 mg / mL, 0.80 to 15 mg / mL, 0.90 to 15 mg / mL, 1 to 15 mg / mL, 1.5 to 15 mg / mL, 2 to 15 mg / mL, 2.5 to 15 mg / mL, 3 to 15 mg / mL, 3.5 to 15 mg / mL, 4 to 15 mg / mL, 4.5 to 15 mg / mL, 5 to 15 mg / mL, 5.5 to 15 mg / mL, 6 to 15 mg / mL, 6.5 to 15 mg / mL, 7 to 15 mg / mL, 7.5 to 15 mg / mL, 8 to 15 mg / mL. g / mL, 0.50~12mg / mL, 0.60~12mg / mL, 0.70~12mg / mL, 0.75~12mg / mL, 0.80~12mg / mL, 0.90~12mg / mL, 1~12mg / mL, 1.5~12mg / mL, 2~12mg / mL, 2.5~ 12mg / mL, 3~12mg / mL, 3.5~12mg / mL, 4~12mg / mL, 4.5~12mg / mL, 5~12mg / mL, 5.5~12mg / mL, 6~12mg / mL, 6.5~12mg / mL, 7~12mg / mL, 7.5~12mg / mL, 8 ~12mg / mL, 0.50~10mg / mL, 0.60~10mg / mL, 0.70~10mg / mL, 0.75~10mg / mL, 0.80~10mg / mL, 0.90~10mg / mL, 1~10mg / mL, 1.5~10mg / mL, 2~10mg / mL, 2.5~10mg / mL, 3~10mg / mL, 3.5~10mg / mL, 4~10mg / mL, 4.5~10mg / mL, 5~10mg / mL, 5.5~10mg / mL, 6~10mg / mL, 6.5~10mg / mL, 7~10mg / mL, 7.5~10mg / mL, 8~10mg / mL, 0.50~8mg / mL, 0.60~8mg / mL, 0.70~8mg / mL, 0.75~8mg / mL, 0.80~8mg / mL, 0.90~8mg / mL, 1~8mg / mL, 1.5~8mg / mL, 2~8mg / mL, 2.5~8 mg / mL, 3~8mg / mL, 3.5~8mg / mL, 4~8mg / mL, 4.5~8mg / mL, 5~8mg / mL, 5.5~8mg / mL, 6~8mg / mL, 6.5~8mg / mL, 7~8mg / mL, 7.5~8mg / mL, 0.50~7mg / mL, 0.60~7mg / mL, 0.70~7mg / mL, 0.75~7mg / mL, 0.80~7mg / mL, 0.90~7mg / mL, 1~7mg / mL, 1.5~7mg / mL, 2~7mg / mL , 2.5~7mg / mL, 3~7mg / mL, 3.5~7mg / mL, 4~7mg / mL, 4.5~7mg / mL, 5~7mg / mL, 5.5~7mg / mL, 6~7mg / mL, 6.5~7m g / mL, 0.50~6mg / mL, 0.60~6mg / mL, 0.70~6mg / mL, 0.75~6mg / mL, 0.80~6mg / mL, 0.90~6mg / mL, 1~6mg / mL, 1 .5~6mg / mL, 2~6mg / mL, 2.5~6mg / mL, 3~6mg / mL, 3.5~6mg / mL, 4~6mg / mL, 4.5~6mg / mL, 5~6mg / mL, 5.5~6mg / mL, 0.50~4mg / mL, 0.60~4mg / mL, 0.70~4mg / mL, 0.75~4mg / mL, 0.80~4mg / mL, 0.90~4mg / mL, 1~4mg / mL, 1.5 ~4mg / mL, 2~4mg / mL, 2.5~4mg / mL, 3~4mg / mL, 3.5~4mg / mL, 0.50~3mg / mL, 0.60~3mg / mL, 0.70~3mg / mL, 0.7 The carnosine content may be 5-3 mg / mL, 0.80-3 mg / mL, 0.90-3 mg / mL, 1-3 mg / mL, 1.5-3 mg / mL, 2-3 mg / mL, 2.5-3 mg / mL, 0.50-2 mg / mL, 0.60-2 mg / mL, 0.70-2 mg / mL, 0.75-2 mg / mL, 0.80-2 mg / mL, 0.90-2 mg / mL, 1-2 mg / mL, or 1.5-2 mg / mL. The carnosine content is preferably 0.75-7 mg / mL, more preferably 0.80-4 mg / mL.

[0030] The carnosine content can be determined by measuring it using high-performance liquid chromatography (HPLC), but if the content can be calculated from the formulation amount, it can also be determined from that. When measuring with HPLC, the measurement conditions can be determined using the standard measurement method of the analyzer. When measuring with HPLC, an ion exchange column (for example, 300SCX (dimensions: 4.6 × 250 mm, particle size: 5 μm)) can be used.

[0031] The ratio of carnosine to the above-mentioned at least one cobalamin is not particularly limited, but in a preferred embodiment of the present invention, the weight ratio of the carnosine content to the content of the above-mentioned at least one cobalamin is 500:1 to 50000:1. In another embodiment of the present invention, the weight ratio of the carnosine content to the content of the above-mentioned at least one cobalamin may be 1000:1 to 50000:1, 2000:1 to 50000:1, 4000:1 to 50000:1, 5000:1 to 50000:1, 8000:1 to 50000:1, 8000:1 to 40000:1, 8000:1 to 35000:1, or 8000:1 to 30000:1. Furthermore, from the viewpoint of improving the undesirable taste quality of carnosine, the weight ratio of carnosine content to the content of at least one of the above-mentioned cobalamins is preferably 4000:1 to 15000:1, more preferably 6000:1 to 12000:1, and even more preferably 6500:1 to 10000:1.

[0032] <Chicken extract> A beverage according to one aspect of the present invention contains chicken extract. Chicken extract is an ingredient obtained by hot water extraction or the like using parts of a chicken, such as meat, bones, and skin, as raw materials. Chicken extract generally contains carnosine.

[0033] In one embodiment of the present invention, when the chicken extract used in a beverage contains a salt of carnosine, the carnosine content in the chicken extract refers to the total amount of carnosine and its salts.

[0034] Examples of carnosine salts are, but are not limited to, inorganic salts such as hydroxides, carbonates and bicarbonates of sodium, lithium, calcium, magnesium and aluminum and ammonia; as well as organic salts such as mono-, di- or tri-alkylamine salts, mono-, di- or tri-hydroxyalkylamine salts, guanidine salts and N-methylglucosamine salts, such as salts of methylamine, dimethylamine and trimethylamine.

[0035] In one embodiment of the present invention, when using chicken extract in a beverage, the extract obtained by heating chicken meat or other raw materials in a liquid may be used, or a commercially available chicken extract may be used. The raw materials for the chicken extract may include chicken bones, cartilage, feet (also called 'momiji'), etc.

[0036] Commercially available chicken extracts include "Brand's Essence of Chicken (BEC) (manufactured by Suntory Beverage & Food Asia)", "Scotch® Essence of Chicken (manufactured by Scotch Industrial, Thailand)", "Quaker Essence of Chicken (manufactured by Standard Foods Corporation, Taiwan)", "SWANSON® Chicken stock and broth (manufactured by Campbell Soup Company)", "Drip Chicken Essence (manufactured by Eu Yan Sang International, Singapore)", "Boned Chicken Tonic (manufactured by Eu Yan Sang International, Singapore)", and "Boiled Essence of Chicken (manufactured by Lao Xie Zhen, Taiwan)". Any of these commercially available products can be used, but Brand's Essence of Chicken (BEC) is preferred.

[0037] When producing chicken extract used in a beverage according to one aspect of the present invention by hot water extraction of chicken meat, chicken bones, cartilage, feet, etc. (hereinafter referred to as "chicken meat, etc."), it can be produced by a generally used method. For example, chicken extract can be produced by treating chicken meat, etc. in a solvent at high temperature under atmospheric pressure or under pressure. When producing chicken extract, the concentration may be increased by removing fat and cholesterol after crude extraction in a solvent, and then removing excess solvent.

[0038] In this specification, chicken extract includes liquid extracts, diluted solutions, concentrates, or dried powders obtained by the above method, and their purified products. Purified products can be obtained, for example, by subjecting chicken extract to diatomaceous earth filtration, ultrafiltration, membrane treatment, liquid separation, or fractionation using resins to achieve high purity.

[0039] In one embodiment of the present invention, the amount of chicken extract in the beverage (on a solids basis) is not particularly limited, but is preferably 5% to 20% by weight. In a preferred embodiment of the present invention, the amount of chicken extract (on a solids basis) may be 6% to 14% by weight, 8% to 12% by weight, or 9% to 11% by weight.

[0040] <Form of beverage> The beverage of the present invention is a packaged beverage. The form of the beverage container is not limited, and the beverage may be packaged in containers such as cans, bottles, PET bottles, pouches, paper cartons, and plastic containers. According to a preferred embodiment of the present invention, the beverage container is a small pouch from the viewpoint of easy and regular consumption. When heat sterilization is performed after packaging, the type is not particularly limited, and can be carried out using conventional methods such as inversion sterilization, UHT sterilization, and retort sterilization. The temperature of the heat sterilization process is not particularly limited, but for example, 65 to 130°C, preferably 80 to 120°C, for 1 to 40 minutes. However, if a sterilization value equivalent to the above conditions can be obtained, sterilization at an appropriate temperature for a few seconds, for example, 5 to 30 seconds, is also acceptable.

[0041] In one embodiment of the present invention, beverages include ready-to-drink (RTD) beverages, soft drinks, soups containing meat extracts (extracts obtained from chicken, beef, or pork, etc.), such as chicken soup, jelly-like beverages, powdered beverages, and functional beverages. In this specification, powdered beverages mean powdered beverages intended to be diluted with water or hot water to a predetermined concentration for consumption. Furthermore, beverages containing chicken extract also include compositions intended to be heated and consumed in a soup-like state, such as pouch-packaged soups (Chicken Essence or DEOC (Drip Essence of Chicken)). In a preferred embodiment of the present invention, the packaged beverage is a ready-to-drink (RTD) beverage.

[0042] <Propylene glycol> A beverage according to one aspect of the present invention contains propylene glycol, while a beverage according to another aspect of the present invention does not contain propylene glycol. If propylene glycol is included, its content may be, for example, less than 1 mg / mL, less than 0.8 mg / mL, less than 0.6 mg / mL, less than 0.4 mg / mL, less than 0.3 mg / mL, or less than 0.2 mg / mL. A beverage according to a second aspect of the present invention contains carnosine and at least one of the above-mentioned cobalamins, and has a propylene glycol content of less than 0.3 mg / mL. The propylene glycol content is not particularly limited as long as it is less than 0.3 mg / mL, and may be 0 mg / mL (or less than the lower limit of detection), but may be, for example, 0.15 mg / mL or less, 0.12 mg / mL or less, 0.10 mg / mL or less, or 0.08 mg / mL or less. Preferably, the propylene glycol content is 0 to 0.08 mg / mL. The amount of propylene glycol can be adjusted by selecting the raw materials to be added. For example, by not adding fragrances, the amount of propylene glycol can be reduced. The propylene glycol content can be measured by HS-SPME-GC-MS (headspace solid-phase microextraction gas chromatography-mass spectrometry).

[0043] <Other ingredients and properties> As described in Patent Document 1, since ethanol promotes the breakdown of cobalamin, it is preferable to limit its content. The ethanol content is preferably less than 0.001 mg / mL, and may be less than 0.0008 mg / mL or less than 0.0005 mg / mL. The amount of ethanol can be adjusted by selecting the raw materials to be added. For example, the amount of ethanol can be reduced by not adding fragrance. The ethanol content can be measured by HS-SPME-GC-MS.

[0044] A beverage according to one aspect of the present invention is substantially free of copper and / or vitamin C. In this specification, "substantially free of copper" means that the iron content is less than 1 mM, and "substantially free of vitamin C" means that the vitamin C content is less than 10 mM. According to one aspect of the present invention, the copper content in the beverage is less than 0.1 mM, and the vitamin C content is less than 1 mM.

[0045] A beverage according to one aspect of the present invention contains caramel. A beverage according to another aspect of the present invention does not contain melon juice. A beverage according to yet another aspect of the present invention does not contain niacinamide, or the niacinamide content is less than 26 mg / 100 ml, less than 20 mg / 100 ml, or less than 15 mg / 100 ml. A beverage according to yet another aspect of the present invention does not contain vitamin B2 (riboflavin), or the vitamin B2 content is less than 3.0 mg / 100 ml, less than 2.0 mg / 100 ml, or less than 1.5 mg / 100 ml. A beverage according to yet another aspect of the present invention does not contain pyridoxine hydrochloride, or the pyridoxine hydrochloride content is less than 5.0 mg / 100 ml, less than 4.0 mg / 100 ml, or less than 2.0 mg / 100 ml.

[0046] A beverage according to one aspect of the present invention is a low-calorie beverage. Here, a low-calorie beverage means, for example, a beverage with a calorie content of 20 kcal / bottle or less, preferably 18 kcal / bottle or less, and more preferably 16 kcal / bottle or less. The calorie content of a beverage can be calculated by calculating the content of each ingredient contained in the beverage and multiplying it by an energy conversion factor, or by measuring the physical heat of combustion using a calorimeter (e.g., a bomb calorimeter) and correcting it with the digestion absorption rate and the amount of heat excreted.

[0047] A beverage according to one aspect of the present invention comprises water. The water is not particularly limited, and any water can be used as long as it does not adversely affect the flavor, but examples include tap water, ion-exchanged water, soft water, distilled water, carbonated water, reverse osmosis water (RO water), activated carbon treated water, purified water, and desalinated water.

[0048] The pH of the beverage according to one embodiment of the present invention may preferably be 4-7, 4.5-7, 5-7, 5.5-7, 6-7, 4-6.8, 4.5-6.8, 5-6.8, 5.5-6.8, 6-6.8, 4-6.6, 4.5-6.6, 5-6.6, 5.5-6.6, or 6-6.6. By adjusting the pH within this range, a desirable taste with less acidity can be achieved.

[0049] The Brix (soluble solids content) of a beverage according to one aspect of the present invention is preferably 5 to 20, more preferably 6 to 14, and even more preferably 8 to 12. The Brix (soluble solids content) of a beverage can be measured using a sugar refractometer.

[0050] A beverage according to one aspect of the present invention may contain a sweetener. The sweetener may include a low-intensity sweetener, a high-intensity sweetener, or a combination thereof. Examples of low-intensity sweeteners include glucose, sucrose (such as granulated sugar), fructose, maltose, oligosaccharides, fructose-glucose syrup, lactose, psicose, allose, tagatose, xylose, or ribose. Examples of high-intensity sweeteners include aspartame, neotame, advantame, sucralose, acesulfame potassium (acesulfame K), saccharin, sodium saccharin, sodium cyclamate, dulcin, disodium glycyrrhizinate, trisodium glycyrrhizinate, neohesperidin dihydrochalcone, thaumatin, monellin, curculin, mavinrin, blazein, pentadine, hernandulcin, 4β-hydroxyhernandulcin, miraculin, glycyrrhizin, rubusoside, phyllodulcin, mogroside IV, mogroside V, steviol glycosides (rebaudioside A, rebaudioside D, rebaudioside M), thaumatin, monellin, blazein, monatin, monatin, monk fruit extract, or stevia extract.

[0051] A beverage according to one aspect of the present invention may contain ingredients other than those mentioned above that can be added to ordinary beverages, as long as the effects of the present invention are not impaired. Examples of such ingredients include acidulants, flavorings, vitamins, colorants, antioxidants, emulsifiers, preservatives, seasonings, extracts, pH adjusters, and quality stabilizers.

[0052] [Exemplary embodiments of the beverage of the present invention] The following are exemplary embodiments of the beverage of the present invention, but the present invention is not limited to these embodiments. According to one aspect of the present invention, carnosine and vitamin B 12 A packaged beverage containing, The carnosine content is 0.70 to 15 mg / mL, preferably 0.75 to 7 mg / mL, and more preferably 0.80 to 4 mg / mL. A packaged beverage is provided, containing at least one cobalamin selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin, at a concentration of 0.005 to 1 μg / mL. According to one aspect of the present invention, carnosine and vitamin B 12 A packaged beverage containing, The carnosine content is 0.70 to 15 mg / mL, preferably 0.75 to 7 mg / mL, and more preferably 0.80 to 4 mg / mL. A packaged beverage containing cyanocobalamin is provided, with a cyanocobalamin content of 0.005 to 1 μg / mL.

[0053] According to one aspect of the present invention, a packaged beverage comprising carnosine and at least one cobalamin selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin, The carnosine content is 0.70 to 15 mg / mL, preferably 0.75 to 7 mg / mL, and more preferably 0.80 to 4 mg / mL. A packaged beverage is provided, wherein the content of at least one of the aforementioned cobalamins is 0.04 to 0.4 μg / mL. According to one aspect of the present invention, a packaged beverage containing carnosine and cyanocobalamin, The carnosine content is 0.70 to 15 mg / mL, preferably 0.75 to 7 mg / mL, and more preferably 0.80 to 4 mg / mL. A packaged beverage containing cyanocobalamin at a concentration of 0.04 to 0.4 μg / mL is provided.

[0054] According to one aspect of the present invention, a packaged beverage comprising carnosine and at least one cobalamin selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin, Contains chicken extract, The carnosine content is 0.70 to 15 mg / mL, preferably 0.75 to 7 mg / mL, and more preferably 0.80 to 4 mg / mL. A packaged beverage is provided, having a cobalamin content of 0.005 to 1 μg / mL. According to one aspect of the present invention, a packaged beverage containing carnosine and cyanocobalamin, Contains chicken extract, The carnosine content is 0.70 to 15 mg / mL, preferably 0.75 to 7 mg / mL, and more preferably 0.80 to 4 mg / mL. A packaged beverage containing cyanocobalamin is provided, with a cyanocobalamin content of 0.005 to 1 μg / mL.

[0055] According to one aspect of the present invention, a packaged beverage comprising carnosine and at least one cobalamin selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin, Contains chicken extract, The carnosine content is 0.75-7 mg / mL. A packaged beverage is provided, wherein the content of at least one of the cobalamins is 0.005 to 1 μg / mL, preferably 0.02 to 0.4 μg / mL, more preferably 0.04 to 0.4 μg / mL, or 0.005 to 0.09 μg / mL. According to one aspect of the present invention, a packaged beverage containing carnosine and cyanocobalamin, Contains chicken extract, The carnosine content is 0.75-7 mg / mL. A packaged beverage is provided, having a cyanocobalamin content of 0.005 to 1 μg / mL, preferably 0.02 to 0.4 μg / mL, more preferably 0.04 to 0.4 μg / mL, or 0.005 to 0.09 μg / mL.

[0056] According to one aspect of the present invention, a packaged beverage comprising carnosine and at least one cobalamin selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin, The carnosine content is 0.50-15 mg / mL. The content of at least one of the aforementioned cobalamins is 0.005 to 1 μg / mL, and A packaged beverage is provided, having a propylene glycol content of less than 0.3 mg / mL, preferably 0.15 mg / mL or less, or 0.08 mg / mL or less. According to one aspect of the present invention, a packaged beverage containing carnosine and cyanocobalamin, The carnosine content is 0.50-15 mg / mL. The cyanocobalamin content is 0.005 to 1 μg / mL, and A packaged beverage is provided, having a propylene glycol content of less than 0.3 mg / mL, preferably 0.15 mg / mL or less, or 0.08 mg / mL or less.

[0057] According to one aspect of the present invention, a packaged beverage comprising carnosine and at least one cobalamin selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin, The carnosine content is 0.50-15 mg / mL. The content of at least one of the aforementioned cobalamins is 0.005 to 1 μg / mL, and The propylene glycol content is 0.15 mg / mL or less. The following requirements: (i) Does not contain melon juice; (ii) Does not contain niacinamide, or contains less than 26 mg / 100 ml of niacinamide; (iii) Does not contain vitamin B2 or contains less than 3.0 mg / 100 ml of vitamin B2; and (iv) Does not contain pyridoxine hydrochloride or contains less than 5.0 mg / 100 ml of pyridoxine hydrochloride. A packaged beverage that satisfies one or more of the following conditions will be provided. According to one aspect of the present invention, a packaged beverage containing carnosine and cyanocobalamin, The carnosine content is 0.50-15 mg / mL. The cyanocobalamin content is 0.005 to 1 μg / mL, and The propylene glycol content is 0.15 mg / mL or less. The following requirements: (i) Does not contain melon juice; (ii) Does not contain niacinamide, or contains less than 26 mg / 100 ml of niacinamide; (iii) Does not contain vitamin B2 or contains less than 3.0 mg / 100 ml of vitamin B2; and (iv) Does not contain pyridoxine hydrochloride or contains less than 5.0 mg / 100 ml of pyridoxine hydrochloride. A packaged beverage that satisfies one or more of the following conditions will be provided.

[0058] 2. Method of manufacturing beverages As one aspect of the present invention, the present invention provides a method for producing a packaged beverage (hereinafter also referred to as "the method for producing the beverage of the present invention"), which comprises carnosine and at least one cobalamin selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin, wherein the carnosine content is 0.70 to 15 mg / mL and the content of the at least one cobalamin is 0.005 to 1 μg / mL.

[0059] A method for producing a beverage according to one aspect of the present invention includes adding at least one of the above-mentioned cobalamins and at least one of the above-mentioned carnosine to a beverage base. A preferred method for producing a beverage according to the present invention includes adding water to the carnosine of the present invention and at least one of the above-mentioned cobalamins to obtain a packaged beverage having a carnosine content of 0.70 to 15 mg / mL and a cobalamin content of 0.005 to 1 μg / mL.

[0060] In one embodiment of the present invention, a method for producing a beverage allows for the addition of a predetermined amount of carnosine to the beverage by adding a food ingredient rich in carnosine. The food ingredients rich in carnosine can be those described in section "1. Beverages containing cobalamin and carnosine" as appropriate.

[0061] In this specification, "beverage base" means "a liquid that serves as a precursor to a beverage," and a beverage is produced by adding predetermined ingredients to the beverage base and, if necessary, undergoing predetermined processing, heat sterilization, etc.

[0062] A manufacturing method according to one aspect of the present invention may include a packaging step and a subsequent sterilization step. Packaging can be carried out by any known method. When heat sterilization is performed after packaging, the type is not particularly limited and can be carried out using conventional methods such as UHT sterilization and retort sterilization. The temperature of the heat sterilization step is not particularly limited, but is for example 50 to 130°C, preferably 50 to 120°C, for 10 to 40 minutes. However, if a sterilization value equivalent to the above conditions can be obtained, sterilization at an appropriate temperature for a few seconds, for example 5 to 30 seconds, is also acceptable.

[0063] In the method for producing the beverage of the present invention, the "cobalamin," "carnosine," "chicken extract," "propylene glycol," and "other components" can be those described in section "1. Beverage containing cobalamin and carnosine." Furthermore, the form and characteristics of the beverage can also be determined based on those described in section "1. Beverage containing cobalamin and carnosine."

[0064] 3. Methods for stabilizing cobalamin As one aspect of the present invention, the present invention provides the following method for stabilizing cobalamin (hereinafter also referred to as "the stabilization method of the present invention"). According to one aspect of the present invention, a method is provided for stabilizing at least one cobalamin selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin, wherein carnosine is contained in a solution containing the at least one cobalamin at a concentration of 0.01 to 15 mg / mL.

[0065] To our surprise, the inventors have discovered that the stability of cobalamin is improved by adding carnosine to a solution containing cobalamin at a predetermined concentration. For example, by using the stabilization method according to one aspect of the present invention, the decomposition of cobalamin during heat treatment such as retort sterilization can be suppressed.

[0066] In one embodiment of the present invention, the stabilization method is such that the concentration of carnosine is 0.01 to 15 mg / mL in a solution containing at least one of the above-mentioned cobalamins. In another embodiment, the concentration of carnosine is such that it is 0.05 to 15 mg / mL, 0.10 to 15 mg / mL, 0.20 to 15 mg / mL, 0.30 to 15 mg / mL, 0.40 to 15 mg / mL, 0.50 to 15 mg / mL, 0.60 to 15 mg / mL, 0.70 to 15 mg / mL, 0.75 to 15 mg / mL, 0.80 to 15 mg / mL, 0.90 to 15 mg / mL, 1 to 15 mg / mL, 1.5 to 15 mg / mL, 2 to 15 mg / mL, 2.5 to 15 mg / mL, 3 to 15 mg / mL, 3.5 to 15 mg / mL, 4 to 15mg / mL, 4.5~15mg / mL, 5~15mg / mL, 5.5~15mg / mL, 6~15mg / mL, 6.5~15mg / mL, 7~15mg / mL, 7.5~15mg / mL, 8~15mg / mL, 0.01~12mg / mL, 0.05~1 2mg / mL, 0.10~12mg / mL, 0.20~12mg / mL, 0.30~12mg / mL, 0.40~12mg / mL, 0.50~12mg / mL, 0.60~12mg / mL, 0.70~12mg / mL, 0.75~12mg / mL, 0.80~ 12mg / mL, 0.90~12mg / mL, 1~12mg / mL, 1.5~12mg / mL, 2~12mg / mL, 2.5~12mg / mL, 3~12mg / mL, 3.5~12mg / mL, 4~12mg / mL, 4.5~12mg / mL, 5~12mg / mL, 5.5~12mg / mL, 6~12mg / mL, 6.5~12mg / mL, 7~12mg / mL, 7.5~12mg / mL, 8~12mg / mL, 0.01~10mg / mL, 0.05~10mg / mL, 0.10~10mg / mL, 0.20~10 mg / mL, 0.30~10mg / mL, 0.40~10mg / mL, 0.50~10mg / mL, 0.60~10mg / mL, 0.70~10mg / mL, 0.75~10mg / mL, 0.80~10mg / mL, 0.90~10mg / mL, 1~10mg / mL, 1.5~10mg / mL, 2~10mg / mL, 2.5~10mg / mL, 3~10mg / mL, 3.5~10mg / mL, 4~10mg / mL, 4.5~10mg / mL, 5~10mg / mL, 5.5~10mg / mL, 6~10mg / mL, 6.5~10mg / mL、7~10mg / mL、7.5~10mg / mL、8~10mg / mL、0.01~8mg / mL、0.05~8mg / mL、0.10~8mg / mL、0.20~8mg / mL、0.30~8mg / mL、0.40~8mg / mL、0.50~8mg / mL、0.60~8mg / mL、0.70~8mg / mL、0.75~8mg / mL、0.80~8mg / mL、0.90~8mg / mL、1~8mg / mL、1.5~8mg / mL、2~8mg / mL、2.5~8mg / mL、3~8mg / mL、3.5~8mg / mL、4~8mg / mL、4.5~8mg / mL、5~8mg / mL、5.5~8mg / mL、6~8mg / mL、6.5~8mg / mL、7~8mg / mL、7.5~8mg / mL、0.01~7mg / mL、0.05~7mg / mL、0.10~7mg / mL、0.20~7mg / mL、0.30~7mg / mL、0.40~7mg / mL、0.50~7mg / mL、0.60~7mg / mL、0.70~7mg / mL、0.75~7mg / mL、0.80~7mg / mL、0.90~7mg / mL、1~7mg / mL、1.5~7mg / mL、2~7mg / mL、2.5~7mg / mL、3~7mg / mL、3.5~7mg / mL、4~7mg / mL、4.5~7mg / mL、5~7mg / mL、5.5~7mg / mL、6~7mg / mL、6.5~7mg / mL、0.01~6mg / mL、0.05~6mg / mL、0.10~6mg / mL、0.20~6mg / mL、0.30~6mg / mL、0.40~6mg / mL、0.50~6mg / mL、0.60~6mg / mL、0.70~6mg / mL、0.75~6mg / mL、0.80~6mg / mL、0.90~6mg / mL、1~6mg / mL、1.5~6mg / mL、2~6mg / mL、2.5~6mg / mL、3~6mg / mL、3.5~6mg / mL、4~6mg / mL、4.5~6mg / mL、5~6mg / mL、5.5~6mg / mL、6~6mg / mL、6.5~6mg / mL、7~6mg / mL、7.5~6mg / mL、8~6mg / mL、0.01~4mg / mL、0.05~4mg / mL、0.10~4mg / mL、0.20~4mg / mL、0.30~4mg / mL、0.40~4mg / mL、0.50~4mg / mL、0.60~4mg / mL、0.70~4mg / mL、0.75~4mg / mL, 0.80~4mg / mL, 0.90~4mg / mL, 1~4mg / mL, 1.5~4mg / mL, 2~4mg / mL, 2.5~4mg / mL, 3~4mg / mL, 3.5~4mg / mL, 0.01~3mg / mL, 0.05~3 mg / mL, 0.10~3mg / mL, 0.20~3mg / mL, 0.30~3mg / mL, 0.40~3mg / mL, 0.50~3mg / mL, 0.60~3mg / mL, 0.70~3mg / mL, 0.75~3mg / mL, 0.80~3mg / mL The concentration may be 0.90-3 mg / mL, 1-3 mg / mL, 1.5-3 mg / mL, 2-3 mg / mL, 2.5-3 mg / mL, 0.01-2 mg / mL, 0.05-2 mg / mL, 0.10-2 mg / mL, 0.20-2 mg / mL, 0.30-2 mg / mL, 0.40-2 mg / mL, 0.50-2 mg / mL, 0.60-2 mg / mL, 0.70-2 mg / mL, 0.75-2 mg / mL, 0.80-2 mg / mL, 0.90-2 mg / mL, 1-2 mg / mL, or 1.5-2 mg / mL. Preferably, the carnosine concentration may be 0.75-7 mg / mL or 0.80-4 mg / mL. One embodiment of the present invention includes adding carnosine to achieve the above concentrations.

[0067] When adding carnosine to a solution containing at least one of the above-mentioned cobalamins, it may be added by extracting it from a carnosine-containing material (e.g., fish or chicken) with an aqueous solvent, by adding it by chemical synthesis, or by adding a carnosine-containing food ingredient to the beverage.

[0068] In a stabilization method according to one aspect of the present invention, the concentration of the at least one cobalamin in the solution containing the above-mentioned at least one cobalamin is not particularly limited, but may be, for example, 0.005 to 1 μg / mL, 0.01 to 1 μg / mL, 0.02 to 1 μg / mL, 0.03 to 1 μg / mL, 0.04 to 1 μg / mL, 0.05 to 1 μg / mL, 0.06 to 1 μg / mL, 0.07 to 1 μg / mL, 0.08 to 1 μg / mL, 0.09 to 1 μg / mL, 0.1 to 1 μg / mL, 0.15 to 1 μg / mL, 0.2 to 1 μg / mL, 0.25 to 1 μg / mL, 0.3~1μg / mL, 0.35~1μg / mL, 0.4~1μg / mL, 0.005~0.8μg / mL, 0.01~0.8μg / mL, 0.02~0.8μg / mL, 0.03~0.8μg / mL, 0.04~0.8μg / mL, 0.05~0.8μg / mL, 0.06~0.8μg / mL, 0.07~0.8μg / mL, 0.08~0.8μg / mL, 0.09~0.8μg / mL, 0.1~0.8μg / mL, 0.15~0.8μg / mL, 0.2~0.8μg / mL, 0.25~0.8μg / mL, 0.3~0.8μg / mL, 0.35 ~0.8μg / mL, 0.4~0.8μg / mL, 0.005~0.6μg / mL, 0.01~0.6μg / mL, 0.02~0.6μg / mL, 0.03~0.6μg / mL, 0.04~0.6μg / mL, 0.05~0.6μg / mL, 0.06~0.6μg / mL, 0.0 7~0.6μg / mL, 0.08~0.6μg / mL, 0.09~0.6μg / mL, 0.1~0.6μg / mL, 0.15~0.6μg / mL, 0.2~0.6μg / mL, 0.25~0.6μg / mL, 0.3~0.6μg / mL, 0.35~0.6μg / mL, 0.4~0 .6μg / mL, 0.005~0.5μg / mL, 0.01~0.5μg / mL, 0.02~0.5μg / mL, 0.03~0.5μg / mL, 0.04~0.5μg / mL, 0.05~0.5μg / mL, 0.06~0.5μg / mL, 0.07~0.5μg / mL, 0.08 ~0.5μg / mL, 0.09~0.5μg / mL, 0.1~0.5μg / mL, 0.15~0.5μg / mL, 0.2~0.5μg / mL , 0.25~0.5μg / mL, 0.3~0.5μg / mL, 0.35~0.5μg / mL, 0.4~0.5μg / mL, 0.005~0.4μg / mL, 0.01~0.4μg / mL, 0.02~0.4μg / mL, 0.03~0.4μg / mL, 0.04~0.4μg / mL, 0.05~0.4μg / mL, 0.06~0 .4μg / mL, 0.07~0.4μg / mL, 0.08~0.4μg / mL, 0.09~0.4μg / mL, 0.1~0.4μg / mL, 0.15~0.4μg / mL, 0.2~0.4 μg / mL, 0.25~0.4μg / mL, 0.3~0.4μg / mL, 0.35~0.4μg / mL, 0.005~0.2μg / mL, 0.01~0.2μg / mL, 0.02~0. 2μg / mL, 0.03~0.2μg / mL, 0.04~0.2μg / mL, 0.05~0.2μg / mL, 0.06~0.2μg / mL, 0.07~0.2μg / mL, 0.08~0. 2μg / mL, 0.09~0.2μg / mL, 0.1~0.2μg / mL, 0.15~0.2μg / mL, 0.005~0.1μg / mL, 0.01~0.1μg / mL, 0.02~0 .1μg / mL, 0.03~0.1μg / mL, 0.04~0.1μg / mL, 0.05~0.1μg / mL, 0.06~0.1μg / mL, 0.07~0.1μg / mL, 0.08~0 The concentration may be 0.1 μg / mL, 0.09-0.1 μg / mL, 0.005-0.09 μg / mL, 0.01-0.09 μg / mL, 0.02-0.09 μg / mL, 0.03-0.09 μg / mL, 0.04-0.09 μg / mL, 0.05-0.09 μg / mL, 0.06-0.09 μg / mL, 0.07-0.09 μg / mL, or 0.08-0.09 μg / mL. The concentration of at least one of the above cobalamins is preferably 0.02-0.4 μg / mL, more preferably 0.04-0.4 μg / mL. This concentration refers to the concentration of the cobalamin contained if the beverage contains only one type of cobalamin, or the total concentration if the beverage contains two or more types of cobalamins.

[0069] In a stabilization method according to one aspect of the present invention, the concentration of cyanocobalamin in the solution containing cyanocobalamin is not particularly limited, but may be, for example, 0.005 to 1 μg / mL, 0.01 to 1 μg / mL, 0.02 to 1 μg / mL, 0.03 to 1 μg / mL, 0.04 to 1 μg / mL, 0.05 to 1 μg / mL, 0.06 to 1 μg / mL, 0.07 to 1 μg / mL, 0.08 to 1 μg / mL, 0.09 to 1 μg / mL, 0.1 to 1 μg / mL, 0.15 to 1 μg / mL, 0.2 to 1 μg / mL, 0.25 to 1 μg / mL, 0.3 to 1 μg / mL, 0.35~1μg / mL, 0.4~1μg / mL, 0.005~0.8μg / mL, 0.01~0.8μg / mL, 0.02~0.8μg / mL, 0.03~0.8μg / mL, 0.04~0.8μg / mL, 0.05~0.8μg / mL, 0.06~0.8μg / mL, 0.0 7~0.8μg / mL, 0.08~0.8μg / mL, 0.09~0.8μg / mL, 0.1~0.8μg / mL, 0.15~0.8μg / mL, 0.2~0.8μg / mL, 0.25~0.8μg / mL, 0.3~0.8μg / mL, 0.35~0.8μg / mL, 0.4~0 .8μg / mL, 0.005~0.6μg / mL, 0.01~0.6μg / mL, 0.02~0.6μg / mL, 0.03~0.6μg / mL, 0.04~0.6μg / mL, 0.05~0.6μg / mL, 0.06~0.6μg / mL, 0.07~0.6μg / mL, 0.08 ~0.6μg / mL, 0.09~0.6μg / mL, 0.1~0.6μg / mL, 0.15~0.6μg / mL, 0.2~0.6μg / mL, 0.25~0.6μg / mL, 0.3~0.6μg / mL, 0.35~0.6μg / mL, 0.4~0.6μg / mL, 0.005~0 .5μg / mL, 0.01~0.5μg / mL, 0.02~0.5μg / mL, 0.03~0.5μg / mL, 0.04~0.5μg / m L, 0.05~0.5μg / mL, 0.06~0.5μg / mL, 0.07~0.5μg / mL, 0.08~0.5μg / mL, 0.09~ 0.5μg / mL, 0.1~0.5μg / mL, 0.15~0.5μg / mL, 0.2~0.5μg / mL, 0.25~0.5μg / mL , 0.3~0.5μg / mL, 0.35~0.5μg / mL, 0.4~0.5μg / mL, 0.005~0.4μg / mL, 0.01~0.4μg / mL, 0.02~0.4μg / mL, 0.03~0.4μg / mL, 0.04~0.4μg / mL, 0.05~0.4μg / mL, 0.06~0.4μg / mL, 0.07 ~0.4μg / mL, 0.08~0.4μg / mL, 0.09~0.4μg / mL, 0.1~0.4μg / mL, 0.15~0.4μg / mL, 0.2~0.4μg / mL, 0.25 ~0.4μg / mL, 0.3~0.4μg / mL, 0.35~0.4μg / mL, 0.005~0.2μg / mL, 0.01~0.2μg / mL, 0.02~0.2μg / mL, 0. 03~0.2μg / mL, 0.04~0.2μg / mL, 0.05~0.2μg / mL, 0.06~0.2μg / mL, 0.07~0.2μg / mL, 0.08~0.2μg / mL, 0.09~0.2μg / mL, 0.1~0.2μg / mL, 0.15~0.2μg / mL, 0.005~0.1μg / mL, 0.01~0.1μg / mL, 0.02~0.1μg / mL, 0.03~0.1μg / mL, 0.04~0.1μg / mL, 0.05~0.1μg / mL, 0.06~0.1μg / mL, 0.07~0.1μg / mL, 0.08~0.1μ The concentration may be g / mL, 0.09-0.1 μg / mL, 0.005-0.09 μg / mL, 0.01-0.09 μg / mL, 0.02-0.09 μg / mL, 0.03-0.09 μg / mL, 0.04-0.09 μg / mL, 0.05-0.09 μg / mL, 0.06-0.09 μg / mL, 0.07-0.09 μg / mL, or 0.08-0.09 μg / mL. The cyanocobalamin concentration is preferably 0.02-0.4 μg / mL, more preferably 0.04-0.4 μg / mL.

[0070] In the stabilization method of the present invention, "cobalamin," "carnosine," "chicken extract," "propylene glycol," and "other components" can be those described in section "1. Beverages containing cobalamin and carnosine."

[0071] 4. How to improve the aftertaste of carnosine As one aspect of the present invention, the present invention provides the following method for improving the aftertaste of carnosine (hereinafter also referred to as "the aftertaste improvement method of the present invention"). According to one aspect of the present invention, a method for improving the aftertaste of carnosine is provided, comprising adding at least one cobalamin selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin to a solution containing 0.01 to 15 mg / mL of carnosine at a concentration of 0.005 to 1 μg / mL.

[0072] To our surprise, the inventors discovered that the undesirable taste characteristics of carnosine can be suppressed by keeping the content of carnosine and at least one of the above-mentioned cobalamins within a predetermined range.

[0073] In one embodiment of the present invention, in a method for improving aftertaste, the concentration of carnosine is 0.01 to 15 mg / mL. In another embodiment, the concentration of carnosine is 0.05 to 15 mg / mL, 0.10 to 15 mg / mL, 0.20 to 15 mg / mL, 0.30 to 15 mg / mL, 0.40 to 15 mg / mL, 0.50 to 15 mg / mL, 0.60 to 15 mg / mL, 0.70 to 15 mg / mL, 0.75 to 15 mg / mL, 0.80 to 15 mg / mL, 0.90 to 15 mg / mL, 1 to 15 mg / mL, 1.5 to 15 mg / mL, 2 to 15 mg / mL, 2.5 to 15 mg / mL, 3 to 15 mg / mL, 3.5 to 15 mg / mL, 4 to 15 mg / mL mL, 4.5~15mg / mL, 5~15mg / mL, 5.5~15mg / mL, 6~15mg / mL, 6.5~15mg / mL, 7~15mg / mL, 7.5~15mg / mL, 8~15mg / mL, 0.01~12mg / mL, 0.05~12mg / mL, 0 .10~12mg / mL, 0.20~12mg / mL, 0.30~12mg / mL, 0.40~12mg / mL, 0.50~12mg / mL, 0.60~12mg / mL, 0.70~12mg / mL, 0.75~12mg / mL, 0.80~12mg / mL, 0.9 0~12mg / mL, 1~12mg / mL, 1.5~12mg / mL, 2~12mg / mL, 2.5~12mg / mL, 3~12mg / mL, 3.5~12mg / mL, 4~12mg / mL, 4.5~12mg / mL, 5~12mg / mL, 5.5~12mg / m L, 6~12mg / mL, 6.5~12mg / mL, 7~12mg / mL, 7.5~12mg / mL, 8~12mg / mL, 0.01~10mg / mL, 0.05~10mg / mL, 0.10~10mg / mL, 0.20~10mg / mL, 0.30~10mg / m L, 0.40~10mg / mL, 0.50~10mg / mL, 0.60~10mg / mL, 0.70~10mg / mL, 0.75~10mg / mL, 0.80~10mg / mL, 0.90~10mg / mL, 1~10mg / mL, 1.5~10mg / mL, 2~1 0mg / mL, 2.5~10mg / mL, 3~10mg / mL, 3.5~10mg / mL, 4~10mg / mL, 4.5~10mg / mL, 5~10mg / mL, 5.5~10mg / mL, 6~10mg / mL, 6.5~10mg / mL, 7~10mg / mL, 7.5~10mg / mL、8~10mg / mL、0.01~8mg / mL、0.05~8mg / mL、0.10~8mg / mL、0.20~8mg / mL、0.30~8mg / mL、0.40~8mg / mL、0.50~8mg / mL、0.60~8mg / mL、0.70~8mg / mL、0.75~8mg / mL、0.80~8mg / mL、0.90~8mg / mL、1~8mg / mL、1.5~8mg / mL、2~8mg / mL、2.5~8mg / mL、3~8mg / mL、3.5~8mg / mL、4~8mg / mL、4.5~8mg / mL、5~8mg / mL、5.5~8mg / mL、6~8mg / mL、6.5~8mg / mL、7~8mg / mL、7.5~8mg / mL、0.01~7mg / mL、0.05~7mg / mL、0.10~7mg / mL、0.20~7mg / mL、0.30~7mg / mL、0.40~7mg / mL、0.50~7mg / mL、0.60~7mg / mL、0.70~7mg / mL、0.75~7mg / mL、0.80~7mg / mL、0.90~7mg / mL、1~7mg / mL、1.5~7mg / mL、2~7mg / mL、2.5~7mg / mL、3~7mg / mL、3.5~7mg / mL、4~7mg / mL、4.5~7mg / mL、5~7mg / mL、5.5~7mg / mL、6~7mg / mL、6.5~7mg / mL、0.01~6mg / mL、0.05~6mg / mL、0.10~6mg / mL、0.20~6mg / mL、0.30~6mg / mL、0.40~6mg / mL、0.50~6mg / mL、0.60~6mg / mL、0.70~6mg / mL、0.75~6mg / mL、0.80~6mg / mL、0.90~6mg / mL、1~6mg / mL、1.5~6mg / mL、2~6mg / mL、2.5~6mg / mL、3~6mg / mL、3.5~6mg / mL、4~6mg / mL、4.5~6mg / mL、5~6mg / mL、5.5~6mg / mL、6~6mg / mL、6.5~6mg / mL、7~6mg / mL、7.5~6mg / mL、8~6mg / mL、0.01~4mg / mL、0.05~4mg / mL、0.10~4mg / mL、0.20~4mg / mL、0.30~4mg / mL、0.40~4mg / mL、0.50~4mg / mL、0.60~4mg / mL、0.70~4mg / mL、0.75~4mg / mL、0.80~4mg / mL、0.90~4mg / mL, 1~4mg / mL, 1.5~4mg / mL, 2~4mg / mL, 2.5~4mg / mL, 3~4mg / mL, 3.5~4mg / mL, 0.01~3mg / mL, 0.05~3mg / mL, 0.10~3mg / mL, 0.20~3mg / mL, 0.30~3mg / mL, 0.40~3mg / mL, 0.50~3mg / mL, 0.60~3mg / mL, 0.70~3mg / mL, 0.75~3mg / mL, 0.80~3mg / mL, 0.90~3mg / mL The concentration may be 1-3 mg / mL, 1.5-3 mg / mL, 2-3 mg / mL, 2.5-3 mg / mL, 0.01-2 mg / mL, 0.05-2 mg / mL, 0.10-2 mg / mL, 0.20-2 mg / mL, 0.30-2 mg / mL, 0.40-2 mg / mL, 0.50-2 mg / mL, 0.60-2 mg / mL, 0.70-2 mg / mL, 0.75-2 mg / mL, 0.80-2 mg / mL, 0.90-2 mg / mL, 1-2 mg / mL, or 1.5-2 mg / mL. Preferably, the carnosine concentration may be 0.40-7 mg / mL or 0.50-4 mg / mL. One embodiment of the present invention includes a stabilization method in which carnosine is added to achieve the above concentrations.

[0074] When adding at least one of the above cobalamins to a carnosine-containing solution, the cobalamin may be added by extracting it from a material containing at least one of the above cobalamins (for example, seafood (especially clams and mussels) or animal meat (e.g., chicken liver)) using an aqueous solvent, or by chemical synthesis, or by adding an ingredient containing at least one of the above cobalamins to a beverage.

[0075] In one embodiment of the present invention, in a method for improving aftertaste, the concentration of at least one of the above-mentioned cobalamins is 0.005 to 1 μg / mL. In another embodiment, the concentration of at least one of the above-mentioned cobalamins is, for example, 0.01 to 1 μg / mL, 0.02 to 1 μg / mL, 0.03 to 1 μg / mL, 0.04 to 1 μg / mL, 0.05 to 1 μg / mL, 0.06 to 1 μg / mL, 0.07 to 1 μg / mL, 0.08 to 1 μg / mL, 0.09 to 1 μg / mL, 0.1 to 1 μg / mL, 0.15 to 1 μg / mL, 0.2 to 1 μg / mL, 0.25 to 1 μg / mL, 0.3 to 1 μg / mL, 0.35 to 1 μg / mL, 0.4 to 1 μg / mL, 0.005 ~0.8μg / mL, 0.01~0.8μg / mL, 0.02~0.8μg / mL, 0.03~0.8μg / mL, 0.04~0.8μg / mL, 0.05~0.8μg / mL, 0.06~0.8μg / mL, 0.07~0.8μg / mL, 0.08~0.8μ g / mL, 0.09~0.8μg / mL, 0.1~0.8μg / mL, 0.15~0.8μg / mL, 0.2~0.8μg / mL, 0.25~0.8μg / mL, 0.3~0.8μg / mL, 0.35~0.8μg / mL, 0.4~0.8μg / mL, 0.00 5~0.6μg / mL, 0.01~0.6μg / mL, 0.02~0.6μg / mL, 0.03~0.6μg / mL, 0.04~0.6μg / mL, 0.05~0.6μg / mL, 0.06~0.6μg / mL, 0.07~0.6μg / mL, 0.08~0.6 μg / mL, 0.09~0.6μg / mL, 0.1~0.6μg / mL, 0.15~0.6μg / mL, 0.2~0.6μg / mL, 0.25~0.6μg / mL, 0.3~0.6μg / mL, 0.35~0.6μg / mL, 0.4~0.6μg / mL, 0.0 05~0.5μg / mL, 0.01~0.5μg / mL, 0.02~0.5μg / mL, 0.03~0.5μg / mL, 0.04~0.5μg / mL, 0.05~0.5μg / mL, 0.06~0.5μg / mL, 0.07~0.5μg / mL, 0.08~0. 5μg / mL, 0.09~0.5μg / mL, 0.1~0.5μg / mL, 0.15~0.5μg / mL, 0.2~0.5μg / mL, 0.25~0.5μg / mL, 0.3~0.5μg / mL, 0.35~0.5μg / mL, 0.4~0.5μg / mL, 0.005~0.4μg / mL, 0.01~0.4μg / mL, 0.02~0.4μg / mL, 0.03~0.4μg / mL, 0.04~0.4μg / mL, 0.05~0.4μg / mL, 0.06~0.4μg / mL, 0. 07~0.4μg / mL, 0.08~0.4μg / mL, 0.09~0.4μg / mL, 0.1~0.4μg / mL, 0.15~0.4μg / mL, 0.2~0.4μg / mL, 0.25~0.4μg / mL, 0.3~0 The concentration may be 0.4 μg / mL, 0.35-0.4 μg / mL, 0.005-0.2 μg / mL, 0.01-0.2 μg / mL, 0.02-0.2 μg / mL, 0.03-0.2 μg / mL, 0.04-0.2 μg / mL, 0.05-0.2 μg / mL, 0.06-0.2 μg / mL, 0.07-0.2 μg / mL, 0.08-0.2 μg / mL, 0.09-0.2 μg / mL, 0.1-0.2 μg / mL, or 0.15-0.2 μg / mL. The concentration of at least one of the above cobalamins is preferably 0.02-0.8 μg / mL, more preferably 0.05-0.6 μg / mL. This concentration refers to the concentration of the cobalamin contained in the beverage if it contains only one type of cobalamin, or the total concentration if the beverage contains two or more types of cobalamin.

[0076] In one aspect of the present invention, the concentration of シノノコココレラMIN containing シアノココレラMIN is not limited, for example, 0.005~1μg / mL, 0.01~1μg / mL, 0.02~1μg / mL, 0.03~1μg / mL, 0.04~1μg / mL, 0.005~1μg / mL. 05~1μg / mL、0.06~1μg / mL、0.07~1μg / mL、0.08~1μg / mL、0.09~1μg / mL、0.1~1μg / mL、0.15~1μg / mL、0.2~1μg / mL、0.25~1μg / mL、0.3~1μg / mL、0.35~1μg / mL L、0.4~1μg / mL、0.005~0.8μg / mL、0.01~0.8μg / mL、0.02~0.8μg / mL、0.03~0.8μg / mL、0.04~0.8μg / mL、0.05~0.8μg / mL、0.06~0.8μg / mL、0.07~0.8μg / mL 、0.08~0.8μg / mL、0.09~0.8μg / mL、0.1~0.8μg / mL、0.15~0.8μg / mL、0.2~0. 8μg / mL、0.25~0.8μg / mL、0.3~0.8μg / mL、0.35~0.8μg / mL、0.4~0.8μg / mL、0. 005~0.6μg / mL、0.01~0.6μg / mL、0.02~0.6μg / mL、0.03~0.6μg / mL、0.04~0.6μg / mL、0.05~0.6μg / mL、0.06~0.6μg / mL、0.07~0.6μg / mL、0.08~0.6μg / mL 、0.09~0.6μg / mL、0.1~0.6μg / mL、0.15~0.6μg / mL、0.2~0.6μg / mL、0.25~0. 6μg / mL、0.3~0.6μg / mL、0.35~0.6μg / mL、0.4~0.6μg / mL、0.005~0.5μg / mL、0 .01~0.5μg / mL、0.02~0.5μg / mL、0.03~0.5μg / mL、0.04~0.5μg / mL、0.05~0. 5μg / mL、0.06~0.5μg / mL、0.07~0.5μg / mL、0.08~0.5μg / mL、0.09~0.5μg / mL、 0.1~0.5μg / mL、0.15~0.5μg / mL、0.2~0.5μg / mL、0.25~0.5μg / mL、0.3~0.5μ g / mL、0.35~0.5μg / mL、0.4~0.5μg / mL、0.005~0.4μg / mL、0.01~0.4μg / mL、0.02~0.4μg / mL, 0.03~0.4μg / mL, 0.04~0.4μg / mL, 0.05~0.4μg / mL, 0.06~0.4μg / mL, 0.07~0.4μg / mL, 0.08~0.4μg / mL, 0.09~0.4μg / mL, 0.1~0.4μg / mL, 0.15~0.4μg / mL, 0.2~0.4μg / mL, 0.25~0.4μg / mL, 0.3~0.4μg / mL, 0.35~0. The concentration may be 4 μg / mL, 0.005-0.2 μg / mL, 0.01-0.2 μg / mL, 0.02-0.2 μg / mL, 0.03-0.2 μg / mL, 0.04-0.2 μg / mL, 0.05-0.2 μg / mL, 0.06-0.2 μg / mL, 0.07-0.2 μg / mL, 0.08-0.2 μg / mL, 0.09-0.2 μg / mL, 0.1-0.2 μg / mL, or 0.15-0.2 μg / mL. The cyanocobalamin concentration is preferably 0.02-0.8 μg / mL, more preferably 0.05-0.6 μg / mL.

[0077] In the aftertaste improvement method of the present invention, the "cobalamin," "carnosine," "chicken extract," "propylene glycol," and "other ingredients" can be those described in section "1. Beverages containing cobalamin and carnosine."

[0078] 5. Inhibitors of cobalamin degradation In one aspect, the present invention provides a cobalamin degradation inhibitor (hereinafter also referred to as "the degradation inhibitor of the present invention"). According to one aspect of the present invention, at least one cobalamin degradation inhibitor is provided, which includes carnosine and is selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin.

[0079] A decomposition inhibitor according to one aspect of the present invention is substantially composed of carnosine. This means that a decomposition inhibitor according to one aspect of the present invention may contain small amounts of components other than carnosine. For example, if the decomposition inhibitor is substantially composed of carnosine, it means that the carnosine content is preferably 85 to 100% by weight, more preferably 90 to 100% by weight, and even more preferably 95 to 100% by weight, based on the total weight of the decomposition inhibitor. A carnosine content of 100% by weight based on the total weight of the decomposition inhibitor means that the decomposition inhibitor is composed of carnosine and the content of any other components is in the form of impurities (less than 1% by weight).

[0080] A decomposition inhibitor according to one aspect of the present invention may include various components that are orally ingestible by humans as other components. For example, it may include a solvent such as water, or a bulking agent or filler.

[0081] The form of the decomposition inhibitor according to one aspect of the present invention is not particularly limited and may be a liquid or a solid.

[0082] In this specification, the phrase "at least" means that the number of a particular item may be greater than or equal to the number listed. Furthermore, in this application, the phrase "about" means that the subject lies within ±25%, ±10%, ±5%, ±3%, ±2%, or ±1% of the number following "about". For example, "about 10" means the range from 7.5 to 12.5. [Examples]

[0083] The present invention will be specifically described below with reference to examples, but the present invention is not limited to the following examples.

[0084] Example 1-1: Stability evaluation of cyanocobalamin Using carnosine (catalog No. 9625, Sigma-Aldrich USA, purity 99% or higher) and cyanocobalamin (catalog No. 0429155, DSM Netherlands, purity 96% or higher), 80 mg / mL carnosine standard solution and 2 mg / mL cyanocobalamin standard solution were prepared, respectively. These standard solutions were diluted to prepare 10-fold stock solutions for experimental sample preparation.

[0085] For each experimental sample, 45 carnosine-cyanocobalamin-containing samples listed in Table 1 were prepared by mixing a 10-fold carnosine stock solution with a 10-fold cyanocobalamin stock solution and diluting it with tap water as appropriate. Similarly, samples with the same composition as samples 1, 10, 19, 28, and 37 were prepared as control samples (without retort sterilization). Since the carnosine used in the test had a purity of 99% or higher, if we assume that all impurities in a sample containing 8 mg / mL of carnosine are propylene glycol, the maximum propylene glycol content is approximately 0.08 mg / mL (approximately 80 ppm). Similarly, since the cyanocobalamin had a purity of 96% or higher, if we assume that all impurities in a sample containing 0.4 μg / mL of cyanocobalamin are propylene glycol, the maximum propylene glycol content is approximately 0.016 μg / mL (approximately 0.016 ppm). [Table 1]

[0086] All experimental samples were subjected to autoclaving at 120°C for 8 minutes. This condition simulated a retort sterilization process. The samples were then cooled to ambient temperature for 2 hours before analysis.

[0087] The amount of cyanocobalamin in each sample after heat treatment was determined by LC-MSMS under the following conditions. For the measurement, a known method was used, and the instrument used was an Agilent 1290 binary LC equipped with an AB Sciex5600 and a triple TOF system. A CORTECS T3 column (120 Å, 1.6 μm, 2.1 mm × 100 mm (Waters)) was used as the liquid chromatography column. Other conditions are as follows: Injected sample volume: 5 μL Mobile phase: Solution A: Water containing 0.1% formic acid Solution B: Acetonitrile containing 0.1% formic acid Column temperature: 40℃ Flow rate: 0.4mL / min Gradient (concentration of solution B) 0-1 minute: Solution B concentration 1% 1-7.5 minutes: Concentration of solution B increases from 1% to 40%. 7.5-10 minutes: Concentration of solution B increases from 40% to 100% 10-12.5 minutes: Solution B concentration 100% → 100% 12.5-13 minutes: Solution B concentration reduced from 100% to 1% 13-15 minutes: Solution B concentration 1% [Table 2]

[0088] The measurement results are shown in Figure 1 and Tables 3 and 4. Table 3 summarizes the measured cyanocobalamin content after the retort test in the presence of carnosine. Table 4 shows the percentage of cyanocobalamin contained in each experimental sample, with the amount of cyanocobalamin in the control sample (unheated) set to 100. Since the control sample was not subjected to heat treatment, the cyanocobalamin contained in the control sample is considered not to have decreased due to thermal decomposition. Therefore, the percentage of cyanocobalamin contained in each experimental sample shown in Table 4 can be considered equivalent to the retention rate of cyanocobalamin after heat treatment. [Table 3]

[0089] [Table 4]

[0090] Furthermore, to verify the stabilizing effect of carnosine on cyanocobalamin, the obtained data is summarized in Figure 2. Figure 2 shows the average data for experimental samples containing cyanocobalamin at concentrations of 0.005 μg / mL, 0.04 μg / mL, 0.05 μg / mL, 0.1 μg / mL, and 0.4 μg / mL, categorized by carnosine concentration.

[0091] Examples 1-2: Stability evaluation of cyanocobalamin in chicken extract-containing beverages Samples containing cyanocobalamin at a concentration of 0.110 μg / mL (11.0 μg / 100 mL) and carnosine at a concentration of 0.77 mg / mL were prepared using cyanocobalamin (catalog No. 0429155, manufactured by DSM Netherlands, purity 96% or higher) and chicken extract (manufactured by Suntory Beverage and Food Thailand, carnosine content: 0.77 g / L). The prepared chicken soup beverage was heat-treated using the same method as in Example 1-1. The cyanocobalamin concentration in the chicken soup beverage before and after heat treatment was measured using the same method as in Example 1-1. As a result, the cyanocobalamin concentration in the chicken soup beverage before heat treatment was 10.9 μg / 100 mL, and the cyanocobalamin concentration after heat treatment was 11.02 μg / 100 mL. From this, it can be understood that almost 100% of the cyanocobalamin was retained before and after heat treatment.

[0092] Example 2: Sensory evaluation of carnosine Samples A-J were prepared by adding carnosine (catalog No. 9625, Sigma-Aldrich USA, purity 99% or higher) and cyanocobalamin (catalog No. 0429155, DSM Netherlands, purity 96% or higher) to water to achieve the compositions shown in Table 5 below. Control samples of samples A-J without cyanocobalamin were also prepared.

[0093] The taste quality of the sample beverages obtained using the above procedure was evaluated. Each sample was evaluated by four expert panelists based on the following criteria. [Sensory evaluation criteria] Rating Criteria 6. The aftertaste is greatly improved. 5. The aftertaste is greatly improved. 4. The aftertaste is improved. 3. The aftertaste is slightly improved. 2. The aftertaste is slightly improved. 1. The aftertaste is slightly improved. 0 No difference from the control sample.

[0094] Before conducting the test, the panelists thoroughly discussed and agreed on the evaluation criteria outlined above. The results are summarized in Table 5. The sensory evaluation values ​​in the table below represent the average scores given by the four panelists. [Table 5]

Claims

1. Carnosine and, At least one cobalamin selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin A packaged beverage containing, The carnosine content is 0.70 to 15 mg / mL. A packaged beverage having a cobalamin content of 0.005 to 1 μg / mL.

2. A packaged beverage according to claim 1, comprising chicken extract.

3. The containerized beverage according to claim 1 or 2, wherein the at least one cobalamin comprises cyanocobalamin.

4. A packaged beverage according to claim 1 or 2, wherein the carnosine content is 0.75 to 7 mg / mL.

5. A packaged beverage according to claim 1 or 2, wherein the carnosine content is 0.80 to 4 mg / mL.

6. The packaged beverage according to claim 1 or 2, wherein the content of at least one of the cobalamins is 0.04 to 0.4 μg / mL.

7. The packaged beverage according to claim 1 or 2, wherein the weight ratio of the carnosine content to the content of at least one cobalamin is 500:1 to 50000:

1.

8. The packaged beverage according to claim 1 or 2, wherein after a retort test at 120°C for 8 minutes, the amount of at least one cobalamin is maintained at 80% by weight or more compared to the amount of at least one cobalamin before the retort test.

9. A packaged beverage according to claim 1 or 2, which is a ready-to-drink (RTD) beverage, a soft drink, chicken soup, a jelly-like beverage, or a functional beverage.

10. A method for producing a packaged beverage according to claim 1 or 2, A method comprising adding at least one of the aforementioned cobalamin and at least one of carnosine to a beverage base.

11. A method to improve the aftertaste of carnosine, In a solution containing 0.01 to 15 mg / mL of carnosine, A method comprising containing at least one cobalamin selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin, such that the concentration is 0.005 to 1 μg / mL.

12. Carnosine and, At least one cobalamin selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin A packaged beverage containing, The carnosine content is 0.50 to 15 mg / mL. The content of at least one of the cobalamins is 0.005 to 1 μg / mL, and A bottled beverage containing less than 0.3 mg / mL of propylene glycol.

13. A packaged beverage according to claim 1 or 12, wherein the ethanol content is less than 0.001 mg / mL.

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